Biaxial double-swing plastic bag cell bioreactor

Abstract

The present invention relates to the technical field of cell bioreactors, and particularly relates to a double-axis double-swing plastic bag cell bioreactor. The present invention includes: a swing box, an elastic swing rod, a swing frame, a swing basket, a multi-functional heat preservation tank, a plastic culture bag, a load-bearing shaft, a swing shaft, a support frame, a toothed belt drive system and a simple pendulum bob system; the swing box is installed on the support frame; the swing box is connected to the swing frame through the elastic swing rod; the swing frame is connected to the upper part of the swing basket; the lower part of the swing basket is clamped and installed on the load-bearing shaft through a swing basket shaft hoop; the multi-functional heat preservation tank is arranged in the swing basket; the plastic culture bag is arranged in the multi-functional heat preservation tank; two sets of the toothed belt drive systems are respectively located on both sides of the swing frame; two sets of the simple pendulum bob systems are respectively installed on both sides of the swing shaft. The present invention has remarkable effects and is conducive to popularization and application.

Description

Technical Field

[0001] The present invention relates to the technical field of cell bioreactors, and particularly to a biaxial double-swing plastic bag cell bioreactor. Background Art

[0002] At present, for the research and development and capacity expansion in the fields of COVID-19 vaccines, diagnostic reagents, antibody drugs, and cell therapy, a large amount of capital, manpower, and technology have been invested globally in the industrialization process of biopharmaceutical research and development and capacity expansion. Therefore, due to the disposable cell bioreactor for large-scale and high-density culture of animal cells, which is the most important upstream bioprocess equipment in the biopharmaceutical industry, it has become the key link and bottleneck problem for capacity expansion. The disposable cell bioreactor largely determines the production capacity, product variety, and product cost of biopharmaceutical enterprises. The currently used swing-type cell bioreactor in biopharmaceutical enterprises has many defects and deficiencies. For example, after the volume of the swing-type cell bioreactor becomes larger, the impact force received when swinging to the two end points in the left and right directions is very large, resulting in severe vibration and noise. Therefore, it can only reach a volume of 50 liters, and it is not possible to make it larger. For this reason, the manufacturer of this reactor stopped producing swing-type cell bioreactors with a volume of more than 50 liters two years ago. If the swing angle is reduced and the swing frequency is decreased to reduce the impact force at the two end points on the left and right, it will cause a decrease in the dissolved oxygen level and flow velocity of the mixed culture solution in the culture bag, thereby seriously affecting cell growth and amplification. It will also cause cell sedimentation due to poor fluidity of the culture solution, and then the cells will adhere to each other, resulting in the rupture of the cell membrane and cell death.

[0003] The information disclosed in this background art section is only intended to deepen the understanding of the overall background art of the present invention, and should not be regarded as an admission or any form of implication that this information constitutes prior art known to those skilled in the art. Summary of the Invention

[0004] The purpose of the present invention is to provide a biaxial double-swing plastic bag cell bioreactor to solve the technical problems existing in the existing swing-type cell bioreactor.

[0005] To achieve the above object, the present invention adopts the following technical solutions: The present invention provides a biaxial double-swing plastic bag cell bioreactor, which includes: a swing box, an elastic swing rod, a swing frame, a swing basket, a multi-functional heat preservation tank, a plastic culture bag, a load-bearing shaft, a swing shaft, a support frame, a toothed belt drive system and a simple pendulum bob system; the swing box is installed on the support frame; the swing box is connected to the swing frame through the elastic swing rod; the swing frame is connected to the upper part of the swing basket; the lower part of the swing basket is clamped and installed on the load-bearing shaft through a swing basket shaft hoop; the multi-functional heat preservation tank is arranged in the swing basket; the plastic culture bag is arranged in the multi-functional heat preservation tank; two sets of the toothed belt drive systems are respectively located on both sides of the swing frame; the upper end of the toothed belt drive system is connected to the swing shaft; the lower end of the toothed belt drive system is connected to the load-bearing shaft; both ends of the swing shaft are installed on two support seats of the support frame; two sets of the simple pendulum bob systems are respectively installed on both sides of the swing shaft.

[0006] As a further technical solution, the swing box includes: a swing box cover, a swing box body, a top short shaft, a thrust joint bearing combined spherical mechanism, a housing, a linear guide rail, a ball screw, a stepping motor, a box bottom plate and a box flange; the swing box cover and the swing box body are connected through the box flange; the stepping motor is arranged on the box bottom plate; the stepping motor is connected to the ball screw; the nut of the ball screw is connected to the housing of the thrust joint bearing combined spherical mechanism; the thrust joint bearing combined spherical mechanism is installed on a sliding table on the linear guide rail; the linear guide rail is installed on the box bottom plate; the thrust joint bearing combined spherical mechanism is penetrated with the top short shaft; the box bottom plate is provided with a hollow part along the direction of the linear guide rail; the lower end of the top short shaft extends out of the hollow part and is connected to the elastic swing rod.

[0007] As a further technical solution, the thrust joint bearing combined spherical mechanism includes: a thrust joint bearing, a housing, a bushing and a top short shaft; thrust joint bearings are respectively arranged at the upper and lower ends of the housing; the bushing is located between the thrust joint bearings at the upper and lower ends; the top short shaft is sequentially penetrated through the thrust joint bearing at the upper end, the bushing and the thrust joint bearing at the lower end. The inner hole of the bushing is matched with the surface of the top short shaft, so that the top short shaft can move up and down; the outer shaft surface of the bushing is in transitional fit with the inner hole of the thrust joint bearing, the inner rings of the two thrust joint bearings are symmetrically assembled, and the inner ring and the outer ring of the thrust joint bearing are in spherical fit and can rotate three-dimensionally.

[0008] As a further technical solution, the elastic swing rod comprises: a swing rod, an upper disc, four tension springs, a swing rod cross shaft, swing rod cross shaft bearings, tension spring fixing rings and a lower disc; the upper part of the swing rod passes through the central hole of the upper disc and is connected to the top short shaft of the swing box; the lower part of the swing rod is connected to the swing rod cross shaft; the swing rod cross shaft is installed on the lower disc through the swing rod cross shaft bearings; the upper ends of the four tension springs are respectively connected to the four corners of the upper disc; the lower ends of the four tension springs are respectively connected to the periphery of the lower disc through the tension spring fixing rings; the lower disc is connected to the swing frame square plate of the swing frame.

[0009] As a further technical solution, the swing frame comprises: a swing frame square plate, swing frame beams, fastening bolt holes, a swing frame bottom plate; the swing frame square plate and the four swing frame beams are bolted together; the swing frame bottom plate is welded to the swing frame beams; the swing frame bottom plate is provided with fastening bolt holes for bolt connection with the upper frame of the swing basket; the swing frame square plate is provided with fastening bolt holes for bolt connection with the lower disc of the elastic swing rod.

[0010] As a further technical solution, the swing basket is provided with a swing basket shaft hoop; the upper part of the swing basket shaft hoop is connected to the bottom of the swing basket by bolts; the swing basket shaft hoop is clamped and fixed on the load-bearing shaft and swings integrally with the load-bearing shaft.

[0011] As a further technical solution, the multi-functional heat preservation tank is connected to the swing basket through fixed platforms at both ends; the inner surface of the multi-functional heat preservation tank is set as an arc surface adapted to the plastic culture bag; the upper part of the multi-functional heat preservation tank has an inwardly contracting arc structure; the multi-functional heat preservation tank is fixed in the swing basket; the multi-functional heat preservation tank is heated by an electric heating rubber plate or a hot water jacket; the cross section of the plastic culture bag is set as an ellipse; the plastic film seam of the plastic culture bag is set at the position of its upper and lower diagonals; the upper part of the plastic culture bag is provided with a plurality of liquid inlets, liquid outlets, air inlets, air outlets, and interfaces for installing dissolved oxygen, pH value and temperature sensors, etc.; the plastic culture bag is made by welding a five-layer co-extruded or seven-layer co-extruded plastic film.

[0012] As a further technical solution, the toothed belt drive system includes: a load-bearing shaft, a load-bearing shaft sliding bearing, a support base, a load-bearing shaft key, a load-bearing shaft toothed belt pulley, a toothed belt, a swing shaft toothed belt pulley, a swing shaft sliding bearing, a swing shaft key, and a swing shaft; the simple pendulum bob system includes: a pendulum rod and a pendulum bob; the load-bearing shaft is installed on the two support bases through the load-bearing shaft sliding bearing; all loads on the load-bearing shaft act on the ground through the bottom surfaces of the two support bases; the load-bearing shaft toothed belt pulley is connected to the load-bearing shaft through the load-bearing shaft key; the two shaft ends of the swing shaft are installed on the two support seats of the support frame through the swing shaft sliding bearing; the swing shaft toothed belt pulley is installed on the swing shaft through the swing shaft key; the swing shaft toothed belt pulley is connected to the load-bearing shaft toothed belt pulley through the toothed belt; the upper end of the pendulum rod is connected to the swing shaft; the lower end of the pendulum rod is connected to the pendulum bob.

[0013] As a further technical solution, the support frame includes: a top frame beam, two end support frames, two end support plates, a top frame cross beam, and a support frame bottom plate; the top frame beam is connected to the support frame bottom plate through the two end support frames; the top frame cross beam is connected to the top frame beam; the two side surfaces of the two end support frames are respectively provided with the two end support plates; the two ends of the swing shaft are connected to the two end support frames through the swing shaft sliding bearing and the support seats.

[0014] As a further technical solution, shock-absorbing rubber pads are provided at the bottom of the support frame bottom plate.

[0015] Adopting the above technical solutions, the present invention has the following beneficial effects:

[0016] The kinetic energy of the pendulum of the present invention is converted into potential energy, and then the potential energy is converted back into kinetic energy of the pendulum, in a cyclic structure and motion principle. A simple pendulum mechanism is added to the existing swinging mechanism to convert the kinetic energy of the pendulum into potential energy, and when swinging back, the potential energy is released and converted into part of the kinetic energy of the pendulum to assist the next reverse swing. Thus, a double-shaft double-swing combination mechanism is formed, which can bring many beneficial effects:

[0017] Firstly, since the kinetic energy of the pendulum is converted into potential energy at the left and right end points, the impact force of the kinetic energy of the ordinary swinging mechanism on the ground, the resulting vibration and noise, and the damage of the impact force to the building structure of the workshop are completely eliminated at the left and right end points. The reactor can stably and quietly complete the whole process of culturing cells and can meet the requirements of various environments.

[0018] Second, the conversion between such energies can save most of the energy from being consumed. Just like the pendulum mechanism in a clock can swing in a cycle for a long time. A stepping motor with a relatively small power can be used to drive a reactor with a larger volume to work, saving energy. At the same time, the structural size and weight of the components on the cell reactor can also be reduced, lowering the manufacturing cost of the cell reactor.

[0019] Third, since the impact force, vibration, and noise are eliminated. This completely solves the practical problem that existing swing-type cell bioreactors in the world cannot work and stop production when the volume is above 50 liters, because when swinging to the left and right endpoints, a large impact force, vibration, shaking, and noise are generated. It is estimated that this double-axis double-swing plastic bag cell bioreactor will not generate an impact force even when the working volume reaches 10,000 liters. And the requirements of this reactor for the plant civil structure and sound insulation and shock absorption devices will be reduced a lot. In this way, the input costs of the plant and various shock absorption and noise reduction related devices can also be reduced, thus reducing the total cost of pharmaceutical production. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required to be used in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0021] Figure 1 The front view of the double-axis double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0022] Figure 2 The side view of the double-axis double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0023] Figure 3 The top view of the double-axis double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0024] Figure 4 The side view of the swing basket provided by the embodiment of the present invention;

[0025] Figure 5 The top view of the swing basket provided by the embodiment of the present invention;

[0026] Figure 6 The front view of the swing frame provided by the embodiment of the present invention;

[0027] Figure 7 The top view of the swing frame provided by the embodiment of the present invention;

[0028] Figure 8 The front view of the elastic rocker provided by the embodiment of the present invention;

[0029] Figure 9 The top view of the elastic rocker provided by the embodiment of the present invention;

[0030] Figure 10 The front view of the heat preservation tank body provided by the embodiment of the present invention;

[0031] Figure 11 The top view of the heat preservation tank body provided by the embodiment of the present invention;

[0032] Figure 12 The structural schematic diagram of the thrust joint bearing combined spherical mechanism provided by the embodiment of the present invention;

[0033] Figure 13 The front view of the support base provided by the embodiment of the present invention;

[0034] Figure 14 The top view of the support base provided by the embodiment of the present invention;

[0035] Figure 15 The front view of the toothed belt drive system and the single pendulum bob system provided by the embodiment of the present invention;

[0036] Figure 16 The side view of the toothed belt drive system and the single pendulum bob system provided by the embodiment of the present invention. Detailed implementation manners

[0037] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0038] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0039] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installed", "connected", and "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] The following further describes the specific embodiments of the present invention in detail with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only for the purpose of illustration and explanation of the present invention, and are not intended to limit the present invention.

[0041] As Figures 1 to 16 shown, this embodiment provides a biaxial double-swing plastic bag cell bioreactor, which includes: a swing box 100, an elastic swing rod 200, a swing frame 300, a swing basket 400, a multi-functional heat preservation tank 500, a plastic culture bag 520, a load-bearing shaft 600, a swing shaft 700, a support frame 900, a toothed belt drive system and a simple pendulum bob system 800; the swing box 100 is installed on the support frame 900; the swing box 100 is connected to the swing frame 300 through the elastic swing rod 200; the swing frame 300 is connected to the upper part of the swing basket 400; the lower part of the swing basket 400 is clamped and installed on the load-bearing shaft 600 through a swing basket shaft hoop 420; the multi-functional heat preservation tank 500 is arranged in the swing basket 400; the plastic culture bag 520 is arranged in the multi-functional heat preservation tank 500; two sets of the toothed belt drive systems are respectively located on both sides of the swing frame 300; the upper end of the toothed belt drive system is connected to the swing shaft 700; the lower end of the toothed belt drive system is connected to the load-bearing shaft 600; both ends of the swing shaft 700 are installed on two support seats of the support frame 900; two sets of the simple pendulum bob systems 800 are respectively installed on both sides of the swing shaft 700.

[0042] Preferably, the swing box 100 includes: a swing box cover 110, a swing box body 112, a top short shaft 120, a thrust joint bearing combined spherical mechanism 130, a housing 136, a linear guide rail 150, a ball screw 160, a stepper motor 170, a box bottom plate 118, and a box flange 116; the swing box cover 110 and the swing box body 112 are connected through the box flange 116; the stepper motor 170 is arranged on the box bottom plate 118; the stepper motor 170 is connected to the ball screw 160; the nut of the ball screw 160 is connected to the housing 136 of the thrust joint bearing combined spherical mechanism 130; the thrust joint bearing combined spherical mechanism 130 is installed on a slide on the linear guide rail 150; the linear guide rail 150 is installed on the box bottom plate 118; the thrust joint bearing combined spherical mechanism 130 is penetrated with the top short shaft 120; the box bottom plate 118 is provided with a hollowed-out part 190 along the direction of the linear guide rail 150; the lower end of the top short shaft 120 extends out of the hollowed-out part 190 and is connected to an elastic swing rod 200.

[0043] Preferably, the thrust joint bearing combined spherical mechanism 130 includes: a thrust joint bearing 132, a housing 136, a bushing 134, and a top short shaft 120; thrust joint bearings 132 are respectively arranged at the upper and lower ends of the housing 136; the bushing 134 is located between the thrust joint bearings 132 at the upper and lower ends; the top short shaft 120 is sequentially penetrated through the thrust joint bearing 132 at the upper end, the bushing 134, and the thrust joint bearing 132 at the lower end.

[0044] Preferably, the elastic swing rod 200 includes: a swing rod 210, an upper disc 220, four tension springs 230, a swing rod cross shaft 240, a swing rod cross shaft bearing 242, a tension spring fixing ring 236, and a lower disc 250; the upper part of the swing rod 210 passes through the central hole of the upper disc 220 and is connected to the top short shaft 120 of the swing box 100; the lower part of the swing rod 210 is connected to the swing rod cross shaft 240; the swing rod cross shaft 240 is installed on the lower disc 250 through the swing rod cross shaft bearing 242; the upper ends of the four tension springs 230 are respectively connected to the four corners of the upper disc 220; the lower ends of the four tension springs 230 are respectively connected to the periphery of the lower disc 250 through the tension spring fixing rings 236; the lower disc 250 is connected to the swing frame square plate of the swing frame 300.

[0045] Preferably, the swing frame 300 includes: a swing frame square plate 310, swing frame beams 320, fastening bolt holes 350, and a swing frame bottom plate 360; the swing frame square plate 310 is bolted to the four swing frame beams 320 to form an integral body; the swing frame bottom plate 360 is welded to the swing frame beams 320 to form an integral body; fastening bolt holes are provided on the swing frame bottom plate 360 for bolt connection with the upper frame of the swing basket 400; fastening bolt holes are provided on the swing frame square plate 310 for bolt connection with the lower plate 250 of the elastic swing rod 200.

[0046] Preferably, the swing basket 400 is provided with a swing basket shaft hoop 420; the upper part of the swing basket shaft hoop 420 is connected to the bottom of the swing basket 400 by bolts; the swing basket shaft hoop 420 is clamped and fixed on the load-bearing shaft 600 and swings integrally with the load-bearing shaft 600.

[0047] Preferably, the multi-functional heat preservation tank 500 is connected to the swing basket 400 through fixed platforms at both ends; the inner surface of the multi-functional heat preservation tank 500 is set to an arc surface that matches the plastic culture bag 520; the upper part of the multi-functional heat preservation tank 500 has an inwardly contracting arc structure; the multi-functional heat preservation tank 500 is fixed in the swing basket 400; the multi-functional heat preservation tank 500 is heated by an electric heating rubber plate or a hot water jacket; the cross-section of the plastic culture bag is set to an ellipse; the plastic film seam of the plastic culture bag 520 is set at the position of its upper and lower diagonals; multiple liquid inlets, liquid outlets, air inlets, air outlets, and interfaces for installing dissolved oxygen, pH value, and temperature sensors, etc. are provided on the upper part of the plastic culture bag 520; the plastic culture bag 520 is made by welding five-layer co-extruded or seven-layer co-extruded plastic films.

[0048] Preferably, the toothed belt drive system includes: a load-bearing shaft 600, load-bearing shaft sliding bearings 610, a support base 650, a load-bearing shaft key 620, a load-bearing shaft toothed belt pulley 780, a toothed belt 720, a swing shaft toothed belt pulley 710, swing shaft sliding bearings 760, a swing shaft key 770, and a swing shaft 700; the simple pendulum bob system 800 includes: a pendulum rod and a pendulum bob; the load-bearing shaft 600 is installed on the two support bases 650 through the load-bearing shaft sliding bearings 610; all loads on the load-bearing shaft 600 act on the ground through the bottom surfaces of the two support bases 650; the load-bearing shaft toothed belt pulley 780 is connected to the load-bearing shaft 600 through the load-bearing shaft key 620; both ends of the swing shaft 700 are installed on two support seats of the support frame 900 through swing shaft sliding bearings 760; the swing shaft toothed belt pulley 710 is installed on the swing shaft 700 through a swing shaft key 770; the swing shaft toothed belt pulley 710 is connected to the load-bearing shaft toothed belt pulley 780 through the toothed belt 720; the upper end of the pendulum rod is connected to the swing shaft 700; the lower end of the pendulum rod is connected to the pendulum bob.

[0049] Preferably, the support frame 900 includes: a top frame beam 910, two end support frames 930, two end support plates 920, a top frame cross beam 912, and a support frame bottom plate 940; the top frame beam 910 is connected to the support frame bottom plate 940 through the two end support frames 930; the top frame cross beam 912 is connected to the top frame beam 910; the two end support plates 920 are respectively arranged on the sides of the two end support frames 930; both ends of the swing shaft 700 are connected to the two end support frames 930 through swing shaft sliding bearings 760 and support seats 790.

[0050] Preferably, shock-absorbing rubber pads are provided at the bottom of the support frame bottom plate 940.

[0051] In the double-shaft double-swing plastic bag cell bioreactor provided by the present invention, first, as can be seen from the movement principle of the reactor in the figure, the power source is the stepping motor and the ball screw in the swing box, which push the spherical mechanism of the thrust joint bearing combination installed on the slide table to perform reciprocating linear motion on the linear guide rail. The short shaft in the spherical mechanism drives the upper part of the elastic pendulum rod to swing left and right. Then, the swing basket and the multifunctional heat preservation tank and the plastic culture bag and the mixed culture solution in the bag are driven by the swing frame to swing left and right around the axis of the load-bearing shaft, causing the mixed culture solution in the culture bag to flow reciprocally in the left and right directions, thus completing the main movement of culturing cells in the culture solution.

[0052] Secondly, the swinging basket assembly is clamped and installed on the load-bearing shaft through the swinging basket shaft hoop. This makes the swinging basket assembly and the load-bearing shaft integrated and swing left and right around the axis of the load-bearing shaft. The load-bearing shaft is installed on two support bases through two semi-circular disc-shaped sliding bearings, so the load-bearing shaft is rotatable on the support bases. At the same time, all the loads on the load-bearing shaft also act on the two support bases and are transmitted to the ground through the two support bases;

[0053] Next, the movement of the double-shaft double-swing mechanism is composed of the combination of a swinging mechanism and a single-pendulum mechanism. First: The swinging mechanism is composed of an elastic pendulum rod swinging the swinging basket assembly (the load-bearing shaft and the swinging basket swing together) left and right around the axis of the load-bearing shaft; Second: The single-pendulum mechanism is composed of a pendulum rod and a pendulum swinging left and right around the axis of the swinging shaft (the pendulum rod, the pendulum and the swinging shaft swing together); Third: The toothed belt pulley on the load-bearing shaft is connected to the toothed belt pulley on the swinging shaft through a toothed belt, transmitting the swinging kinetic energy on the load-bearing shaft to the swinging shaft. Then, the pendulum rod and the pendulum connected to the swinging shaft convert the swinging kinetic energy into potential energy through their own reverse swinging. Such cyclic conversion of kinetic energy and potential energy is completed when swinging to the left and right end points. Such a cycle is repeated to complete the whole process of the double-shaft double-swing movement;

[0054] Summary: The above is the structure and motion principle of the present invention in which the swinging kinetic energy is converted into potential energy, and then the potential energy is converted back into swinging kinetic energy, repeating the cycle. That is, a single-pendulum mechanism is added to the existing swinging mechanism to convert the swinging kinetic energy into potential energy, and when swinging back, the potential energy is released and converted into part of the swinging kinetic energy to assist the next reverse swing. Thus, such a double-shaft double-swing combined mechanism is formed, which can bring many beneficial effects:

[0055] First, since the swinging kinetic energy is converted into potential energy at the left and right end points, this completely eliminates the impact force of the swinging kinetic energy on the ground, the resulting vibration and noise, and the damage to the factory building structure caused by the impact force when the ordinary swinging mechanism reaches the left and right end points. This enables the reactor to stably and quietly complete the whole process of culturing cells and meet the requirements of various environments.

[0056] Second, this conversion between energies can save most of the energy from being consumed. Just like the single-pendulum mechanism in a clock that can swing cyclically for a long time. A stepping motor with a smaller power can be used to drive a reactor with a larger volume to work, saving energy. At the same time, it can also reduce the structural size and weight of the components on the cell reactor, reducing the manufacturing cost of the cell reactor.

[0057] Thirdly, since the impact force, vibration and noise are eliminated, the practical problem that the existing swing-type cell bioreactor in the world cannot work and stop production when the volume is more than 50 liters, due to the large impact force, vibration, shaking and noise generated when swinging to the left and right end points, is completely solved. It is estimated that there will be no impact force even when the working volume reaches 10,000 liters. And the requirements of this reactor for the civil engineering structure of the plant and the sound insulation and shock absorption devices will be reduced a lot. In this way, the investment costs of the plant and various shock absorption and noise reduction related devices can also be reduced, thereby reducing the total cost of pharmaceutical production.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A biaxial double-swing plastic bag cell bioreactor, characterized in that, Including: Swing box (100), elastic pendulum rod (200), swing frame (300), swing basket (400), multi-functional heat preservation tank (500), plastic culture bag (520), load-bearing shaft (600), swing shaft (700), support frame (900), toothed belt drive system and simple pendulum bob system (800); The swing box (100) is installed on the support frame (900); the swing box (100) is connected to the swing frame (300) through the elastic pendulum rod (200); the swing frame (300) is connected to the upper part of the swing basket (400); the lower part of the swing basket (400) is clamped and installed on the load-bearing shaft (600) through the swing basket shaft hoop (420); the multi-functional heat preservation tank (500) is arranged in the swing basket (400); the plastic culture bag (520) is arranged in the multi-functional heat preservation tank (500); two sets of the toothed belt drive systems are respectively located on both sides of the swing frame (300); the upper end of the toothed belt drive system is connected to the swing shaft (700); the lower end of the toothed belt drive system is connected to the load-bearing shaft (600); both ends of the swing shaft (700) are installed on two support seats of the support frame (900); two sets of the simple pendulum bob systems (800) are respectively installed on both sides of the swing shaft (700); the swing basket (400) is provided with a swing basket shaft hoop (420); the upper part of the swing basket shaft hoop (420) is connected to the bottom of the swing basket (400) through bolts; the swing basket shaft hoop (420) is clamped and fixed on the load-bearing shaft (600) and swings integrally with the load-bearing shaft (600); the simple pendulum bob system (800) includes: a pendulum rod and a pendulum bob; the upper end of the pendulum rod is connected to the swing shaft (700); the lower end of the pendulum rod is connected to the pendulum bob.

2. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The swing box (100) includes: a swing box cover (110), a swing box body (112), a top short shaft (120), a thrust joint bearing combined spherical mechanism (130), a housing (136), a linear guide rail (150), a ball screw (160), a stepping motor (170), a box body bottom plate (118), and a box body flange (116); the swing box cover (110) is connected to the swing box body (112) through the box body flange (116); the stepping motor (170) is arranged on the box body bottom plate (118); the stepping motor (170) is connected to the ball screw (160); the nut of the ball screw (160) is connected to the housing (136) of the thrust joint bearing combined spherical mechanism (130); the thrust joint bearing combined spherical mechanism (130) is installed on the slide table of the linear guide rail (150); the linear guide rail (150) is installed on the box body bottom plate (118); the thrust joint bearing combined spherical mechanism (130) is penetrated with the top short shaft (120); the box body bottom plate (118) is provided with a hollow part (190) along the direction of the linear guide rail (150); the lower end of the top short shaft (120) extends out of the hollow part (190) and is connected to an elastic swing rod (200).

3. The biaxial double-swing plastic bag cell bioreactor according to claim 2, characterized in that, The thrust joint bearing combined spherical mechanism (130) includes: a thrust joint bearing (132), a housing (136), a bushing (134), and a top short shaft (120); Thrust joint bearings (132) are respectively arranged at the upper and lower ends of the housing (136); The bushing (134) is located between the thrust joint bearings (132) at the upper and lower ends; The top short shaft (120) is sequentially penetrated through the thrust joint bearing (132) at the upper end, the bushing (134), and the thrust joint bearing (132) at the lower end.

4. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The elastic swing rod (200) includes: a swing rod (210), an upper disc (220), four tension springs (230), a swing rod cross shaft (240), swing rod cross shaft bearings (242), tension spring fixing rings (236), and a lower disc (250); The upper part of the swing rod (210) passes through the central hole of the upper disc (220) and is connected to the top short shaft (120) of the swing box (100); the lower part of the swing rod (210) is connected to the swing rod cross shaft (240); the swing rod cross shaft (240) is installed on the lower disc (250) through the swing rod cross shaft bearings (242); the upper ends of the four tension springs (230) are respectively connected to the four corners of the upper disc (220); the lower ends of the four tension springs (230) are respectively connected to the periphery of the lower disc (250) through the tension spring fixing rings (236); the lower disc (250) is connected to the swing frame square plate of the swing frame (300).

5. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The swing frame (300) includes: a swing frame square plate (310), swing frame beams (320), fastening bolt holes (350), and a swing frame bottom plate (360); The swing frame square plate (310) is bolted to the four swing frame beams (320) to form an integral body; the swing frame bottom plate (360) is welded to the swing frame beams (320); the swing frame bottom plate (360) is provided with fastening bolt holes for bolt connection with the upper frame of the swing basket (400); the swing frame square plate (310) is provided with fastening bolt holes for bolt connection with the lower plate (250) of the elastic swing rod (200).

6. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The multifunctional heat preservation tank (500) is connected to the swing basket (400) through fixed platforms at both ends; the inner surface of the multifunctional heat preservation tank (500) is set to an arc surface adapted to the plastic culture bag (520); the upper part of the multifunctional heat preservation tank (500) has an inwardly contracting arc structure; the multifunctional heat preservation tank (500) is fixed in the swing basket (400); the multifunctional heat preservation tank (500) is heated by an electric heating rubber plate or a hot water jacket; the cross-section of the plastic culture bag is set to an ellipse; the plastic film seam of the plastic culture bag (520) is set at the position of its upper and lower diagonals; the upper part of the plastic culture bag (520) is provided with a plurality of liquid inlets, liquid outlets, air inlets, air outlets, and interfaces for installing dissolved oxygen, pH value and temperature sensors; the plastic culture bag (520) is made by welding five-layer co-extruded or seven-layer co-extruded plastic films.

7. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The toothed belt drive system includes: a load-bearing shaft (600), a load-bearing shaft sliding bearing (610), a support base (650), a load-bearing shaft key (620), a load-bearing shaft toothed belt pulley (780), a toothed belt (720), a swing shaft toothed belt pulley (710), a swing shaft sliding bearing (760), a swing shaft key (770), and a swing shaft (700); the load-bearing shaft (600) is installed on the two support bases (650) through the load-bearing shaft sliding bearing (610); all loads on the load-bearing shaft (600) act on the ground through the bottom surfaces of the two support bases (650); the load-bearing shaft toothed belt pulley (780) is connected to the load-bearing shaft (600) through the load-bearing shaft key (620); the two ends of the swing shaft (700) are installed on the two support seats of the support frame (900) through swing shaft sliding bearings (760); the swing shaft toothed belt pulley (710) is installed on the swing shaft (700) through a swing shaft key (770); the swing shaft toothed belt pulley (710) is connected to the load-bearing shaft toothed belt pulley (780) through the toothed belt (720).

8. The biaxial double-swing plastic bag cell bioreactor according to claim 1, characterized in that, The support frame (900) includes: a top frame beam (910), two end support frames (930), two end support plates (920), a top frame cross beam (912), and a support frame bottom plate (940); the top frame beam (910) is connected to the support frame bottom plate (940) through the two end support frames (930); the top frame cross beam (912) is connected to the top frame beam (910); the two end support plates (920) are respectively arranged on the sides of the two end support frames (930); both ends of the swing shaft (700) are connected to the two end support frames (930) through swing shaft sliding bearings (760) and support seats (790).

9. The biaxial double-swing plastic bag cell bioreactor according to claim 8, characterized in that, A shock-absorbing rubber pad is arranged at the bottom of the support frame bottom plate (940).

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