A microfluidic detection chip

Abstract

The present invention provides a microfluidic detection chip, comprising a flow channel plate, a micropump, a front cover layer, a back cover layer and a plurality of microvalves, wherein the flow channel plate is provided with a detection cavity, a waste liquid cavity and a plurality of sample addition cavities on the front, and a micropump cavity is provided on the back, the bottom of the sample addition cavity is provided with a liquid inlet hole that passes through the flow channel plate from top to bottom, the micropump is placed in the micropump cavity, the microvalve and the liquid inlet hole have at least one section of complementary shape, when the microvalve is inserted into the liquid inlet hole, the liquid in the sample addition cavity cannot flow through the liquid inlet hole to the flow channel on the back of the flow channel plate. The microfluidic detection chip of the present invention has a stable and reliable microfluidic flow channel, and is equipped with a micropump and a simple and operable microvalve, which can conveniently realize the switching of multi-channel liquid circuits. Through the coordinated work of the microvalve and the micropump, the volume of the sample and other reagents flowing through the detection cavity can be conveniently controlled, and the concentration of the substance to be tested in the sample can be quantitatively measured in a relatively short time.

Description

Technical Field

[0001] The invention belongs to the fields of biochemical detection and microfluidics, and relates to a microfluidics detection chip. Background Art

[0002] Biochemical testing refers to the use of biological or chemical methods to detect target solutions. Microfluidics, also known as a lab on a chip, integrates basic operational units involved in biology, chemistry, and medicine, such as sample preparation, reaction, separation, and detection, onto a single chip with micron-scale microchannels, automating the entire reaction and analysis process.

[0003] How to provide a simple-to-operate microvalve structure to achieve convenient switching between multiple liquid paths in a microfluidic detection chip has become an important technical problem that needs to be solved urgently by those skilled in the art. Summary of the Invention

[0004] The object of the present invention is to provide a microfluidic detection chip for solving the problem of inconvenient switching of multiple liquid paths in microfluidic detection chips in the prior art.

[0005] To achieve the above-mentioned and other related objectives, the present invention provides a microfluidic detection chip, comprising:

[0006] A flow channel plate, wherein a detection cavity, a waste liquid cavity and a plurality of sample addition cavities are provided on the front of the flow channel plate, and a micro pump cavity is provided on the back of the flow channel plate, wherein a liquid inlet hole is provided at the bottom of the sample addition cavity and passes through the flow channel plate vertically, a flow channel connecting hole is provided between the detection cavity and the sample addition cavity and passes through the flow channel plate vertically, the detection cavity and the flow channel connecting hole are communicated with each other through a flow channel provided on the front of the flow channel plate, the flow channel connecting hole and the liquid inlet are communicated with each other through a flow channel provided on the back of the flow channel plate, a waste liquid output hole and a waste liquid input hole are provided on the bottom surface of the micro pump cavity and pass through the flow channel plate vertically, the waste liquid output hole is communicated with the detection cavity, and the waste liquid input hole is communicated with the waste liquid cavity;

[0007] A micropump is placed in the micropump cavity, and a fluid inlet and a fluid outlet are provided on the front of the micropump, wherein the fluid inlet is connected to the waste liquid output hole, and the fluid outlet is connected to the waste liquid input hole;

[0008] a front covering layer, located on the front of the flow channel plate, and covering the detection cavity, the flow channel connection hole, the waste liquid output hole, the waste liquid input hole, and the flow channel on the front of the flow channel plate;

[0009] a back covering layer, located on the back of the flow channel plate and covering the liquid inlet hole, the flow channel connecting hole and the flow channel on the back of the flow channel plate;

[0010] A plurality of microvalves are provided, wherein the microvalves and the liquid inlet holes have at least one section of complementary shape. When the microvalves are inserted into the liquid inlet holes, the liquid in the sample adding cavity cannot flow through the liquid inlet holes to the flow channel on the back of the flow channel plate.

[0011] Optionally, the liquid inlet hole and the microvalve both have a section of inclined sidewalls that are inclined inward from top to bottom. When the microvalve is inserted into the liquid inlet hole, the inclined sidewalls of the liquid inlet hole are in close contact with the inclined sidewalls of the microvalve.

[0012] Optionally, the cross-sections of the liquid inlet and the section of the microvalve having the inclined sidewall are both circular or polygonal.

[0013] Optionally, the side wall of the liquid inlet hole is vertical, and the side wall of the portion of the microvalve inserted into the liquid inlet hole is vertical.

[0014] Optionally, the cross section of the liquid inlet hole and the cross section of the portion of the microvalve inserted into the liquid inlet hole are both circular or polygonal.

[0015] Optionally, the material of the flow channel plate includes acrylic, and the material of the microvalve includes any one of acrylic and polydimethylsiloxane.

[0016] Optionally, the micropump includes any one of a thermal bubble micropump, a syringe pump, a peristaltic pump and a piezoelectric pump.

[0017] Optionally, the front cover layer includes a pressure film, and the back cover layer includes a pressure film.

[0018] As described above, the microfluidic detection chip of the present invention has a stable and reliable microfluidic flow channel and is equipped with a micropump and a simple and easy-to-operate microvalve, which can easily realize the switching of multi-channel liquid circuits. Through the coordinated work of the microvalve and the micropump, the volume of the sample and other reagents flowing through the detection cavity can be conveniently controlled, and the concentration of the substance to be tested in the sample can be quantitatively measured in a relatively short time. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Shown is a top view of the flow channel plate.

[0020] Figure 2 Shown is a bottom view of the flow channel plate.

[0021] Figure 3 Shown is a schematic diagram of the silicon chip used to detect signals.

[0022] Figure 4 Shown is a three-dimensional structural diagram of the micropump.

[0023] Figure 5Shown is a schematic structural diagram of the front cover layer.

[0024] Figure 6 Shown is a schematic structural diagram of the back cover layer.

[0025] Figure 7 Shown is a schematic diagram of the three-dimensional structure of the microvalve.

[0026] Figure 8 Shown is the assembly alignment method of the microfluidic detection chip.

[0027] Figure 9 Shown is a schematic diagram of the structure of the microfluidic detection chip after assembly.

[0028] Figure 10 and Figure 11 Two cross-sectional views of the assembled microfluidic detection chip are shown respectively.

[0029] Figure 12 The cross-sectional view shows the portion of the flow channel plate having the sample adding cavity and the liquid inlet.

[0030] Figure 13 The cross-sectional view shows the microvalve being located above the liquid inlet hole but not inserted into the liquid inlet hole.

[0031] Figure 14 The figure shows a cross-sectional view of the microvalve when inserted into the liquid inlet hole.

[0032] Figure 15 and Figure 16 The schematic diagram shows that the side wall of the liquid inlet hole is in a vertical state, and the side wall of the portion of the microvalve inserted into the liquid inlet hole is also vertical.

[0033] Component number description

[0034] 1 runner plate;

[0035] 101 Detection of cavity;

[0036] 102 waste liquid cavity;

[0037] 103 sample loading cavity;

[0038] 104 micro pump cavity;

[0039] 105 liquid inlet;

[0040] 106 flow channel connection hole;

[0041] 107 flow channel;

[0042] 108 flow channel;

[0043] 109 waste liquid output hole;

[0044] 110 waste liquid input port;

[0045] 111 flow channel;

[0046] 2 micro pumps;

[0047] 201 fluid inlet;

[0048] 202 fluid outlet;

[0049] 3. Front cover layer;

[0050] 301 opening;

[0051] 4. Back cover layer;

[0052] 5 micro valve;

[0053] 6 silicon wafers; DETAILED DESCRIPTION

[0054] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention.

[0055] See also Figures 1 to 16 It should be noted that the diagrams provided in this embodiment are merely schematic illustrations of the basic concept of the present invention. Therefore, the diagrams only show components related to the present invention and are not drawn according to the number, shape, and size of components in actual implementation. In actual implementation, the type, quantity, and proportion of each component may be changed arbitrarily, and the component layout may also be more complex.

[0056] Example 1

[0057] This embodiment provides a microfluidic detection chip, which includes a flow channel plate 1 , a micropump 2 , a front cover layer 3 , a back cover layer 4 and a plurality of microvalves 5 .

[0058] See also Figure 1 and Figure 2 ,in, Figure 1 Shown is a top view of the flow channel plate 1, Figure 2The bottom view of the flow channel plate 1 is shown. The front of the flow channel plate 1 is provided with a detection cavity 101, a waste liquid cavity 102 and a plurality of sample addition cavities 103. The back of the flow channel plate 1 is provided with a micro pump cavity 104. The bottom of the sample addition cavity 103 is provided with a liquid inlet hole 105 that passes through the flow channel plate 1 from top to bottom. A flow channel connection hole 106 that passes through the flow channel plate 1 from top to bottom is provided between the detection cavity 101 and the sample addition cavity 103. The micro-pump cavity 104 is connected through a flow channel 107 provided on the front side of the flow channel plate 1, and the flow channel connecting hole 106 and the liquid inlet hole 105 are connected through a flow channel 108 provided on the back side of the flow channel plate 1. The bottom surface of the micro-pump cavity 104 is provided with a waste liquid output hole 109 and a waste liquid input hole 110 that pass through the flow channel plate 1 from top to bottom. The waste liquid output hole 109 is connected to the detection cavity 101, and the waste liquid input hole 110 is connected to the waste liquid cavity 102.

[0059] Specifically, the detection cavity 101 of the flow channel plate 1 can be used to detect the concentration of the substance to be detected in the unknown solution, for example, the silicon chip 6 (such as Figure 3 As shown in FIG. 1 , the detection device 100 can be placed in the detection cavity 101 of the flow channel plate 1 to complete the required detection.

[0060] As an example, the waste liquid outlet 109 is connected to the detection cavity 101 via a flow channel 111 provided on the front surface of the flow channel plate 1. Each of the flow channel connection holes 106 can be connected to the detection cavity 101 via an independent flow channel or via a flow channel that eventually merges with the detection cavity 101. Figure 1 The figure shows the connection between each channel connection hole 106 and the detection cavity 101 through the final confluent channel 107.

[0061] As an example, the material of the flow channel plate 1 includes but is not limited to acrylic.

[0062] As an example, the size of the flow channel plate 1 is 80mm*60mm*10mm, the size of the sample adding cavity 103 is 10mm*10mm*3mm, the width and height of each flow channel is 1mm*0.2mm, the size of the waste liquid cavity 102 is 15mm*15mm*3mm, and the size of the silicon wafer 6 placed in the detection cavity 101 is 5.2mm*5.2mm*0.5mm.

[0063] It should be pointed out that for different reaction systems, the number of sample loading cavities is not limited to 3. The specific sizes of the flow channel plate and the cavities and flow channels therein can be adjusted according to the number of sample loading cavities and the volume of the solution to be tested. This should not unduly limit the scope of protection of the present invention.

[0064] See also Figure 4, which shows a three-dimensional structural diagram of the micropump 2. The micropump 2 is placed in the micropump cavity 104 on the back of the flow channel plate 1 to drive the fluid. The front of the micropump 2 is provided with a fluid inlet 201 and a fluid outlet 202. The fluid inlet 201 is connected to the waste liquid output hole 109 of the flow channel plate 1 to receive waste liquid flowing out of the detection cavity 101. The fluid outlet 202 is connected to the waste liquid input hole 110 of the flow channel plate 1 to pump waste liquid into the waste liquid cavity 102.

[0065] By way of example, the micropump 2 includes, but is not limited to, any one of a thermal bubble micropump, a syringe pump, a peristaltic pump, and a piezoelectric pump. In this embodiment, a thermal bubble micropump is used as an example of the micropump 2. The fluid inlet and outlet of the thermal bubble micropump have a radius of 0.5 mm and a center-to-center distance of 4.58 mm. In other embodiments, the size and distance of the fluid inlet and outlet can be adjusted as needed, and this should not unduly limit the scope of the present invention.

[0066] See also Figure 5 , which is a schematic structural diagram of the front covering layer 3. The front covering layer 3 is located on the front of the flow channel plate 1 and covers the detection cavity 101, the flow channel connecting hole 106, the waste liquid output hole 109, the waste liquid input hole 110 and the flow channel on the front of the flow channel plate 1.

[0067] As an example, the front cover layer 3 includes but is not limited to a pressure film.

[0068] As an example, the front cover layer 3 is a whole piece, and an opening 301 is provided at a position of the front cover layer 3 opposite to the waste liquid cavity 102 and the sample adding cavity 103 .

[0069] In other embodiments, the front covering layer 3 may also be multiple pieces. For example, the portion covering the detection cavity 101 may be a single piece, so that the other parts of the microfluidic detection chip can be pre-assembled, and a silicon wafer or other carrier for detecting signals can be placed in the detection cavity 101 at a specific moment. The scope of protection of the present invention should not be excessively limited here.

[0070] See also Figure 6 , which is a schematic structural diagram of the back cover layer 4 , wherein the back cover layer 4 is located on the back of the flow channel plate 1 and covers the liquid inlet 105 , the flow channel connection hole 106 and the flow channel on the back of the flow channel plate 1 .

[0071] As an example, the back cover layer 4 includes but is not limited to a pressure film.

[0072] As an example, the back cover layer 4 is a whole piece.

[0073] See also Figure 7 , which shows a schematic diagram of the three-dimensional structure of the microvalve 5. The microvalve 5 and the liquid inlet 105 have at least one complementary shape. When the microvalve 5 is inserted into the liquid inlet 105, the liquid in the sample loading cavity 103 cannot flow through the liquid inlet 105 to the flow channel on the back of the flow channel plate 1, thereby achieving multi-channel fluid path switching. The microvalve 5 can be operated manually or by a robotic arm, and this should not unduly limit the scope of protection of the present invention.

[0074] As an example, the material of the microvalve 5 includes but is not limited to any one of acrylic and polydimethylsiloxane (PDMS).

[0075] See also Figure 8 , showing the assembly alignment method of the microfluidic detection chip.

[0076] As an example, during the assembly of the microfluidic detection chip, the micropump 2 can first be bonded to the micropump cavity 104 through AB glue or other adhesives (such as double-sided tape, heat-curing glue, UV glue, etc.) (wherein, the fluid inlet 201 of the micropump 2 is connected to the waste liquid output hole 109 at the bottom of the micropump cavity 104, and the fluid outlet 202 of the micropump 2 is connected to the waste liquid input hole 110 at the bottom of the micropump cavity 104), and the antibody-modified silicon wafer 6 is placed in the detection cavity 101 (the silicon wafer can also be bonded to the bottom of the detection cavity through an adhesive), and then the pressure membrane (front cover layer 3 and back cover layer 4) is connected to the front and back of the flow channel plate 1 by pressure extrusion.

[0077] See also Figure 9 , which is a schematic diagram of the structure of the microfluidic detection chip after assembly, wherein one microvalve 5 is in a released state to open the corresponding liquid path, and the other microvalves are placed in the corresponding liquid inlet holes to cut off the corresponding liquid paths.

[0078] See also Figure 10 and Figure 11 , which respectively show two cross-sectional views of the assembled microfluidic detection chip.

[0079] See also Figures 12 to 14 ,in, Figure 12 The cross-sectional view of the flow channel plate 1 having the sample adding cavity 103 and the liquid inlet 105 is shown. Figure 13 It is a cross-sectional view showing the microvalve 5 being located above the liquid inlet hole 105 but not inserted into the liquid inlet hole 105. Figure 14 It shows a cross-sectional view of the microvalve 5 when inserted into the liquid inlet hole 105 .

[0080] As an example, the liquid inlet hole 105 and the microvalve 5 both have an inclined side wall that tilts inward from top to bottom, that is, the contact portion between the liquid inlet hole 105 and the microvalve 5 is conical. When the microvalve 5 is inserted into the liquid inlet hole 105, the inclined side wall of the liquid inlet hole 105 is in close contact with the inclined side wall of the microvalve 5.

[0081] As an example, when the microvalve 5 is inserted into the liquid inlet 105, the bottom surface of the microvalve 5 may reach the plane where the bottom surface of the flow channel plate 1 is located, or may not reach the plane where the bottom surface of the flow channel plate 1 is located. Figure 14 As shown, when the microvalve 5 is inserted into the liquid inlet hole 105, the bottom surface of the microvalve 5 does not reach the plane where the bottom surface of the flow channel plate 1 is located, which is more conducive to applying downward pressure to make the microvalve 5 in close contact with the side wall of the liquid inlet hole.

[0082] As an example, the cross-sections of the liquid inlet 105 and the section of the microvalve 5 with the inclined side wall are both circular or polygonal, and correspondingly, the corresponding portion of the microvalve 5 is truncated cone or prism-shaped.

[0083] See also Figure 15 and Figure 16 In another embodiment, the side wall of the liquid inlet hole 105 may also be vertical, and the side wall of the portion of the microvalve 5 inserted into the liquid inlet hole 105 is also vertical.

[0084] As an example, Figure 16 As shown, when the side wall of the liquid inlet hole 105 is vertical, when the microvalve 5 is inserted into the liquid inlet hole 105 and the axes coincide, the distance between the outer wall of the microvalve 5 and the inner wall of the liquid inlet hole 105 is less than 0.02 mm, that is, the size of the microvalve 5 is slightly smaller than the size of the liquid inlet 105, so that the microvalve 5 can be placed in the liquid inlet 105 while achieving the effect of a microvalve by relying on the difference in flow resistance at the liquid inlet 105.

[0085] It should be pointed out that Figure 16 What is presented is a situation where the bottom surface of the microvalve 5 is in contact with the back cover layer 4 when the microvalve 5 is inserted into the liquid inlet hole 105. In other embodiments, when the microvalve 5 is inserted into the liquid inlet hole 105, the bottom surface of the microvalve 5 may not be in contact with the back cover layer 4.

[0086] As an example, the cross section of the liquid inlet hole 105 and the cross section of the portion of the microvalve 5 inserted into the liquid inlet hole 105 are both circular or polygonal, and correspondingly, the corresponding portion of the microvalve 5 is cylindrical or prismatic.

[0087] It should be pointed out that in other embodiments, the contours of the microvalve 5 and the liquid inlet hole 105 may also be other shapes, as long as the shapes are complementary so that the liquid path can be cut off when the microvalve 5 is inserted into the liquid inlet hole 105. The scope of protection of the present invention should not be excessively limited here.

[0088] The microfluidic detection chip of this embodiment has a stable and reliable microfluidic flow channel and is equipped with a micropump and a simple and easy-to-operate microvalve, which can easily realize the switching of multi-channel liquid circuits. Through the coordinated work of the microvalve and the micropump, the volume of the sample and other reagents flowing through the detection cavity can be conveniently controlled, and the concentration of the substance to be tested in the sample can be quantitatively measured in a relatively short time.

[0089] Example 2

[0090] This embodiment uses the microfluidic detection chip described in Example 1 to detect small molecule samples.

[0091] See Figure 9 and Figure 10 The microfluidic detection chip has three liquid inlet cavities, three conical microvalves for switching liquid paths, a flow channel, a reaction cavity where a modified primary antibody silicon wafer is placed, a thermal bubble micropump, and a waste liquid cavity from right to left.

[0092] Add excess sample, labeled secondary antibody, and rinse solution to each of the three inlet chambers. First, use a tapered acrylic microvalve to block the inlet of the inlet chamber containing the secondary antibody and rinse solution. Open the hot bubble micropump until the calculated volume of sample has been dispensed, then close the pump. Second, use the same acrylic microvalve to block the inlet of the inlet chamber containing the sample and labeled secondary antibody. Open the hot bubble micropump to rinse out excess sample, then close the pump. Third, continue using acrylic microvalves to block the inlet of the inlet chamber containing the sample and rinse solution. Open the hot bubble micropump until the calculated volume of secondary antibody has been dispensed, then close the pump. Fourth, repeat the steps in step 2, except this time, rinse out excess secondary antibody. Finally, in step 5, place the microfluidic detection chip in a dedicated fluorescence intensity instrument to read the fluorescence intensity. Each different fluorescence value corresponds to a specific sample concentration.

[0093] Example 3

[0094] In this example, the microfluidic detection chip described in Example 1 was used to perform sandwich enzyme-linked immunosorbent assay (ELISA) sample detection.

[0095] The microfluidic detection chip required in this embodiment is Figure 9 The microfluidic detection chip shown is substantially the same, except that the number of required liquid inlet cavities is changed from three to four.

[0096] The specific detection process is as follows: Antigens, namely, sample reagent, antibody B reagent paired with the primary antibody, secondary antibody reagent, and cleaning solution, are added to each of the four liquid inlet cavities. Following the procedures of Example 2, the sample reagent, cleaning solution, antibody B reagent, cleaning solution, secondary antibody reagent, and cleaning solution are sequentially passed through. The microfluidic detection chip is then placed in a dedicated fluorescence intensity instrument to read the fluorescence value and determine the corresponding sample concentration.

[0097] Example 4

[0098] This example uses the microfluidic detection chip described in Example 1 to perform chemiluminescence immunoassay sample detection.

[0099] The microfluidic detection chip required in this embodiment is Figure 9 The microfluidic detection chip shown is substantially the same, except that the number of required liquid inlet cavities is changed from three to five.

[0100] The specific detection process is as follows: Antigens, namely, sample reagent, antibody B reagent paired with the primary antibody, secondary antibody reagent, luminescent substrate reagent, and cleaning solution, are added to each of the five liquid inlet cavities. Following the procedures of Example 2, the sample reagent, cleaning solution, antibody B reagent, cleaning solution, secondary antibody reagent, cleaning solution, and luminescent substrate reagent are sequentially passed through the microfluidic detection chip, and the reaction is allowed to proceed for a predetermined period of time. The microfluidic detection chip is then placed in a dedicated fluorescence intensity instrument to read the fluorescence value and determine the corresponding sample concentration.

[0101] In summary, the microfluidic detection chip of the present invention features stable and reliable microfluidic channels, equipped with a micropump and easily operable microvalves, enabling convenient switching of multi-channel fluid paths. The coordinated operation of the microvalves and micropumps facilitates control of the volume of sample and other reagents flowing through the detection cavity, enabling quantitative determination of the concentration of the analyte in the sample in a relatively short period of time. Therefore, the present invention effectively overcomes the shortcomings of the prior art and possesses high industrial applicability.

[0102] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A microfluidic detection chip, characterized in that: include: A flow channel plate, wherein a detection cavity, a waste liquid cavity and a plurality of sample addition cavities are provided on the front of the flow channel plate, and a micro pump cavity is provided on the back of the flow channel plate, wherein a liquid inlet hole is provided at the bottom of the sample addition cavity and passes through the flow channel plate vertically, a flow channel connecting hole is provided between the detection cavity and the sample addition cavity and passes through the flow channel plate vertically, the detection cavity and the flow channel connecting hole are communicated with each other through a flow channel provided on the front of the flow channel plate, the flow channel connecting hole and the liquid inlet are communicated with each other through a flow channel provided on the back of the flow channel plate, a waste liquid output hole and a waste liquid input hole are provided on the bottom surface of the micro pump cavity and pass through the flow channel plate vertically, the waste liquid output hole is communicated with the detection cavity, and the waste liquid input hole is communicated with the waste liquid cavity; A micropump is placed in the micropump cavity, and a fluid inlet and a fluid outlet are provided on the front of the micropump, wherein the fluid inlet is connected to the waste liquid output hole, and the fluid outlet is connected to the waste liquid input hole; The micropump includes any one of a thermal bubble micropump, a syringe pump, a peristaltic pump and a piezoelectric pump; a front covering layer, located on the front of the flow channel plate, and covering the detection cavity, the flow channel connection hole, the waste liquid output hole, the waste liquid input hole, and the flow channel on the front of the flow channel plate; a back covering layer, located on the back of the flow channel plate and covering the liquid inlet hole, the flow channel connecting hole and the flow channel on the back of the flow channel plate; a plurality of microvalves, wherein the microvalves and the liquid inlet holes have at least one section of complementary shape, and when the microvalves are inserted into the liquid inlet holes, the liquid in the sample loading cavity cannot flow through the liquid inlet holes to the flow channel on the back of the flow channel plate; The front cover layer includes a pressure film, and the back cover layer includes a pressure film.

2. The microfluidic detection chip according to claim 1, characterized in that: The liquid inlet hole and the microvalve both have a section of inclined sidewalls that are inclined inward from top to bottom. When the microvalve is inserted into the liquid inlet hole, the inclined sidewalls of the liquid inlet hole are in close contact with the inclined sidewalls of the microvalve.

3. The microfluidic detection chip according to claim 2, characterized in that: The cross sections of the liquid inlet hole and the section of the microvalve having the inclined side wall are both circular or polygonal.

4. The microfluidic detection chip according to claim 1, characterized in that: The side wall of the liquid inlet hole is vertical, and the side wall of the portion of the microvalve inserted into the liquid inlet hole is vertical.

5. The microfluidic detection chip according to claim 4, characterized in that: The cross section of the liquid inlet hole and the cross section of the portion of the microvalve inserted into the liquid inlet hole are both circular or polygonal.

6. The microfluidic detection chip according to claim 1, characterized in that: The material of the flow channel plate includes acrylic, and the material of the microvalve includes any one of acrylic and polydimethylsiloxane.

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