Fat extraction cartridge, fat extractor and method of use thereof

Abstract

The present invention relates to a fat extraction cylinder, a fat extractor and a method for using the same. The fat extraction cylinder comprises a fat extraction cylinder body with an opening at the upper end, a water jacket layer is arranged on the outer ring of the fat extraction cylinder body, and a water inlet pipe and a water outlet pipe are connected to the water jacket; an extraction bottom bottle connecting end is arranged at the bottom end of the bottom of the fat extraction cylinder body; a siphon device is connected to the bottom of the fat extraction cylinder body, which is used to continuously reflux the extraction solvent in the fat extraction cylinder body to the extraction bottom bottle connected to the extraction bottom bottle connecting end; a connecting pipe is arranged on the fat extraction cylinder body, one end of the connecting pipe is connected to the extraction bottom bottle connecting end, and the other end is connected to the upper end of the fat extraction cylinder body. It is used for detecting the crude fat content in oil samples, is safe and simple to operate, and can realize rapid detection of batch samples.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural product detection, and in particular relates to a fat extraction for detecting crude fat content in oil and a use method thereof. Background Art

[0002] Soxhlet crude fat extractor is a device commonly used to measure the crude fat content in agricultural products and foods such as oils and grains. It extracts fat according to the principle of Soxhlet method and uses a balance to weigh the mass to determine the crude fat content. Soxhlet fat extraction method is a national standard (GB5009.6-2016) determination method due to its stable performance, high accuracy and easy operation. It has been widely used in the determination of crude fat in food, oil, feed and other industries.

[0003] In the actual sample fat extraction process, the Soxhlet crude fat extractor needs to use ether or petroleum ether as the extraction solvent. The extraction is carried out for a long time in a 60-70℃ water bath. The method of weighing the crude fat mass obtained by extraction is adopted. It can only be used for the test of a single sample. In addition, the ether used to extract the sample is inconvenient to heat during use, the extraction time is long, the energy consumption is high, the reagent consumption and the detection cost are high. In order to adapt to the detection of large quantities of samples in my country's oil industry in breeding, production and circulation links, how to improve the extraction efficiency of the Soxhlet crude fat extractor is a problem that detection and inspection workers are concerned about. Summary of the invention

[0004] The main technical problem solved by the present invention is to provide a fat extraction cylinder, a fat extractor and a method of using the same, which can realize efficient extraction of crude fat in batch oil samples and solve the problem that the existing detection method is only applicable to a small amount of samples and has low extraction efficiency.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] Provided is a fat extraction cylinder, comprising a fat extraction cylinder body with an opening at the upper end, a water jacket layer being arranged on the outer ring of the fat extraction cylinder body, a water inlet pipe and a water outlet pipe being connected to the water jacket layer; an extraction bottom bottle connecting end being arranged at the bottom lower end of the bottom of the fat extraction cylinder body; a siphon device being connected at the bottom of the fat extraction cylinder body, for continuously refluxing the extraction solvent in the fat extraction cylinder body into the extraction bottom bottle connected to the extraction bottom bottle connecting end; a connecting pipe being arranged on the fat extraction cylinder body, one end of the connecting pipe being connected to the extraction bottom bottle connecting end, and the other end being connected to the upper end of the fat extraction cylinder body.

[0007] According to the above scheme, the siphon device includes a first siphon tube connected to the bottom of the fat extraction cylinder body, a spherical cavity structure connected to the first siphon tube, and a second siphon tube arranged on the spherical cavity structure, and the second siphon tube is connected to the extraction bottom bottle connection end.

[0008] According to the above scheme, the spherical cavity structure is close to the upper end of the fat extraction cylinder, a first siphon tube extends from the bottom of the fat extraction cylinder body and is connected upward to the spherical cavity structure, and a second siphon tube extends from the spherical cavity structure and is connected to the extraction bottom bottle connection end.

[0009] According to the above scheme, a straight pressure relief pipe is provided on the spherical cavity structure and is connected to the upper part of the fat extraction cylinder body.

[0010] According to the above scheme, the upper opening of the fat extraction cylinder is a ground mouth, which is convenient for connecting to the condensation device; the extraction bottom bottle connecting end of the fat extraction cylinder at the lower end is a ground mouth, which is convenient for connecting to the extraction bottom bottle.

[0011] According to the above scheme, a solvent recovery nozzle is provided at the bottom of the fat extraction cylinder body.

[0012] According to the above scheme, a rubber hose and a spring clip matching the rubber hose are installed on the solvent recovery nozzle.

[0013] The fat extractor comprises a fat extraction cylinder, an extraction bottom bottle connecting end at the lower end of the fat extraction cylinder is connected to the extraction bottom bottle, and the upper end of the fat extraction cylinder is connected to a condensation device.

[0014] According to the above scheme, the ground mouth of the extraction bottom bottle connecting end at the lower end of the fat extraction cylinder is connected to the extraction bottom bottle, and the upper end opening of the fat extraction cylinder is connected to the condensation tube of the condensation device.

[0015] A method for using the fat extractor is provided, wherein a sample package to be tested is placed in the fat extraction tube body, and an extraction solvent is added to submerge the sample to be extracted;

[0016] Add extraction solvent to the extraction bottom bottle; connect the fat extraction cylinder, extraction bottom bottle and condensation device, and connect the condensation device to running tap water for condensation;

[0017] Connect the water jacket arranged outside the fat extraction cylinder body to the water inlet and outlet of the circulating water heater respectively, adjust the water temperature of the circulating water heater, and keep the hot water circulating in the water jacket of the fat extraction cylinder;

[0018] Place the extraction bottom bottle on a water bath and heat it in a water bath for extraction.

[0019] According to the above scheme, add a few glass balls or pumice stones to the extraction bottom bottle and then add the extraction solvent.

[0020] According to the above scheme, the extraction solvent is added to the fat extraction cylinder so that it exceeds the height of the sample package to be tested by 2cm.

[0021] According to the above scheme, the sample package to be tested is an oil sample package; the extraction solvent is anhydrous ether, the water bath temperature is 60-70°C, so that the condensed ether dripping is in the shape of beads; the water jacket circulating water temperature of the fat extraction cylinder is 50-60°C.

[0022] According to the above scheme, after the extraction is completed, turn off the power supply of the circulating water heater and the water bath, and release the ether in the extraction cylinder device through the solvent recovery nozzle for recovery; take out the sample package to be tested, let the extraction solvent evaporate in a ventilated place, dry it in a drying oven and then weigh it, calculate the weight difference of the oil sample package before and after extraction, and calculate the crude fat content.

[0023] The fat extraction cylinder provided by the present invention is provided with a water jacket layer, which can realize hot water circulation and realize hot extraction extraction by heating the extraction solvent, which well solves the problem that the extraction solvent of the extraction sample is inconvenient to heat. The fat extraction cylinder is provided with a siphon device, which can realize the continuous reflux of the extraction solvent. Specifically, the extraction solvent of the lower layer of the fat extraction cylinder can be continuously refluxed into the extraction bottom bottle through the first siphon tube 7 and then to the second siphon tube 9. The device can continuously replace the fresh extraction solvent in the fat extraction cylinder device, so that the sample package can be continuously immersed and extracted, and can realize continuous hot extraction extraction. It is not only simple in structure, but also can improve the extraction efficiency.

[0024] Beneficial effects of the present invention:

[0025] The fat extraction cylinder of the present invention has a reasonable and ingenious structural design and can simultaneously perform cyclic thermal extraction on oil-containing samples under normal pressure conditions, thereby achieving efficient extraction of crude fat in batch oil samples, and solving the problem that the existing method is only applicable to a small amount of samples and has low extraction efficiency.

[0026] The solvent recovery nozzle arranged at the bottom of the fat extraction cylinder body of the present invention can conveniently realize the safe recovery of the extraction solvent in the extraction process, reduce consumption, and lower the detection cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the structure of the fat extraction cylinder of the present invention;

[0028] Figure 2 The schematic diagram of the overall structure of the fat extractor of the present invention;

[0029] Wherein: 1. Ground mouth on extraction cylinder; 2. Water outlet pipe; 3. Water inlet pipe; 4. Connecting pipe; 5. Solvent recovery nozzle; 6. Fat extraction cylinder body; 7. First siphon; 8. Spherical cavity; 9. Second siphon; 10. Straight pressure relief pipe; 11. Water jacket; 12. Extraction bottom bottle connecting end; 13 Condensation device; 14 Extraction bottom bottle. DETAILED DESCRIPTION

[0030] The present invention is further described below with specific implementations in combination with drawings and methods of use, but the present invention is not limited to these embodiments.

[0031] Fat extraction tube, such as Figure 1As shown, it comprises a fat extraction cylinder body 6 provided with a grinding mouth 1 on the extraction cylinder, a water jacket layer 11 is provided on the outer ring of the fat extraction cylinder body, and a water inlet pipe 3 and a water outlet pipe 2 are connected to the water jacket; a solvent recovery nozzle 5 is provided at the bottom of the fat extraction cylinder body, and a rubber hose and a spring clip matched with the rubber hose are installed on the solvent recovery nozzle. An extraction bottom bottle connecting end 12 is provided at the bottom end of the bottom of the fat extraction cylinder body; a first siphon tube 7 is connected to the bottom of the fat extraction cylinder body, and the first siphon tube is connected to the spherical cavity structure 8, a straight pressure relief tube 10 is provided on the spherical cavity structure, a second siphon tube 9 is provided on the spherical cavity structure and connected to the upper part of the fat extraction cylinder body, and a connecting tube 4 is provided on the fat extraction cylinder body, one end of the connecting tube is connected to the extraction bottom bottle connecting end, and the other end is connected to the upper end of the fat extraction cylinder body.

[0032] The fat extractor comprises the above-mentioned fat extraction cylinder, the extraction bottom bottle connecting end arranged at the lower end of the fat extraction cylinder is connected to the round bottom flask 14, and the ground mouth on the fat extraction cylinder is connected to the condensation device 13.

[0033] Specific usage of the above fat extractor:

[0034] Embodiment 1:

[0035] Step 1. Prepare filter paper packages: Cut the filter paper into 7.5×7.5cm size and fold them into paper packages with one side not sealed. Use pencil to number them and arrange them in order in the culture dish, with no more than 20 packages per dish. Move the culture dish and the filter paper packages into a (105±2)℃ oven and dry for 2h. Take them out and put them into a desiccator to cool to room temperature (about 45-60min, the same below), and put each package into the same weighing bottle to weigh the paper weight (a).

[0036] Step 2: Prepare samples: Use a corner key to carefully pack the prepared rapeseed samples into paper bags, about 1.5g per bag, seal the bags, and arrange them in a petri dish according to the original serial number. Dry them in a (105±2)℃ oven for 3 hours, then cool them in a desiccator to room temperature (about 45-60 minutes). Weigh each bag in a weighing bottle (b).

[0037] Step 3: Sample loading: First install a high-quality rubber tube and a spring clip on the solvent recovery nozzle 5 of the fat extraction cylinder device; put the rapeseed sample package into the inner tube body of the extraction cylinder device, 10 sample packages can be loaded at a time, and then pour 400mL of anhydrous ether to make it 2cm higher than the rapeseed sample package. Add 5-7 pumice stones and 500mL of anhydrous ether to the round bottom bottle, and then, according to the structure diagram Figure 2 Connect the various parts of the fat extractor.

[0038] Step 4, extraction: connect the condensed water flow, install it on the water bath for extraction, and maintain the temperature of the water bath at 65°C; connect the water jacket arranged on the outer ring of the fat extraction cylinder body 6 to the water inlet pipe 3 of the outer tube body of the fat extraction cylinder device and the water outlet pipe 2 of the outer tube body to the water inlet and outlet of the circulating heating water bath, adjust the water temperature of the circulating heating water bath, and circulate the hot water in 11 of the fat extraction cylinder device, and maintain the circulating water temperature at 50°C, so that the ether dripping from the condensation is in the shape of beads; extract for 4 hours, and the extraction can be carried out at room temperature of 10°C to 25°C.

[0039] Step 5: After the extraction is completed, turn off the circulating heating water bath and the water bath pot, and release the remaining ether in the fat extraction cylinder device through the solvent recovery nozzle 5 for recovery. Take out the rapeseed sample bag and evaporate the ether to near dryness in a ventilated place.

[0040] Step 6: Place the preliminarily dried rapeseed sample package in a constant temperature drying oven and adjust the temperature to (105±2)℃ for 2 hours, then place in a dryer to cool to room temperature (approximately 45-60 minutes). Place the rapeseed sample package in a weighing bottle and weigh the sample package (c) to calculate the crude fat content.

[0041] Embodiment 2:

[0042] Step 1. Prepare filter paper packages: Cut the filter paper into 7.5×7.5cm size and fold them into paper packages with one side not sealed. Use pencil to number them and arrange them in order in the culture dish, with no more than 20 packages per dish. Move the culture dish and the filter paper packages into a (105±2)℃ oven and dry for 2h. Take them out and put them into a desiccator to cool to room temperature (about 45-60min, the same below), and put each package into the same weighing bottle to weigh the paper weight (a).

[0043] Step 2: Prepare samples: Use a corner key to carefully pack the prepared peanut samples into paper bags, about 1.5g per bag, seal the bags, and arrange them in a petri dish according to the original serial number. Dry them in a (105±2)℃ oven for 3 hours, then cool them in a desiccator to room temperature (about 45-60 minutes). Weigh each bag in a weighing bottle (b).

[0044] Step 3: Sample loading: Pack the peanut sample into the inner tube of the fat extraction tube. 30 sample packs can be loaded at a time. Pour 400 mL of anhydrous ether into the tube to make it 2 cm higher than the peanut sample pack. Add 7-9 pumice stones and 500 mL of anhydrous ether into the round bottom bottle. Then, follow the structure diagram. Figure 2 Connect the various parts of the fat extractor.

[0045] Step 4, extraction: connect the condensed water flow, install it on the water bath for extraction, and maintain the temperature of the water bath at 67°C; connect the water jacket arranged on the outer ring of the fat extraction cylinder body 6 to the water inlet pipe 3 of the outer tube body of the fat extraction cylinder device and the water outlet pipe 2 of the outer tube body to the water inlet and outlet of the circulating heating water bath, and adjust the water temperature of the circulating heating water bath. Hot water circulates in 11 of the fat extraction cylinder device, and the circulating water temperature is maintained at 55°C, so that the ether dripping from the condensation is in the shape of beads; extract at room temperature for 3.5 hours.

[0046] Step 5: After the extraction is completed, turn off the circulating heating water bath and the water bath pot, and release the remaining ether in the fat extraction cylinder device through the solvent recovery nozzle 5 for recovery. Take out the peanut sample bag and allow the ether to evaporate to near dryness in a ventilated place.

[0047] Step 6. Place the preliminarily dried sample package in a constant temperature drying oven and adjust the temperature to (105±2)℃ for 2 hours, then place in a dryer and cool to room temperature (approximately 45-60 minutes). Place the peanut sample package in a weighing bottle and weigh the sample package (c) to calculate the crude fat content.

[0048] Embodiment 3:

[0049] Step 1. Prepare filter paper packages: Cut the filter paper into 7.5×7.5cm size and fold them into paper packages with one side not sealed. Use pencil to number them and arrange them in order in the culture dish, with no more than 20 packages per dish. Move the culture dish and the filter paper packages into a (105±2)℃ oven and dry for 2h. Take them out and put them into a desiccator to cool to room temperature (about 45-60min, the same below), and put each package into the same weighing bottle to weigh the paper weight (a).

[0050] Step 2: Prepare samples: Use a corner key to carefully put the prepared feed samples into paper bags, about 1.5g per bag, seal the bags, and arrange them in a petri dish according to the original serial number. Dry them in a (105±2)℃ oven for 3 hours, then cool them in a desiccator to room temperature (about 45-60 minutes). Weigh each bag in a weighing bottle (b).

[0051] Step 3: Sample loading: First install a high-quality rubber tube and a spring clip on the solvent recovery nozzle 5 at the bottom of the inner tube of the fat extraction tube device; put the above feed sample packages into the inner tube body of the extraction tube, 20 sample packages can be loaded at a time, and then pour 400mL of anhydrous ether to make it 2cm higher than the feed sample package. Add 5-9 pumice stones and 500mL of anhydrous ether to the round bottom bottle, and then, according to the structure diagram Figure 2 Connect the various parts of the fat extractor.

[0052] Step 4, extraction: connect the condensed water flow, install it on the water bath for extraction, and keep the water bath temperature at 70°C; connect the water jacket arranged on the outer ring of the fat extraction cylinder body 6 to the water inlet pipe 3 and the water outlet pipe 2 of the outer tube body of the fat extraction cylinder device to the water inlet and outlet of the circulating heating water bath, and adjust the water temperature of the circulating heating water bath. Hot water circulates in 11 of the fat extraction cylinder device, and the circulating water temperature is maintained at 55°C, so that the ether dripping from the condensation is in the shape of beads; extract at room temperature for 3 hours.

[0053] Step 5: After the extraction is completed, turn off the circulating heating water bath and the water bath pot, and release the remaining ether in the fat extraction cylinder device through the solvent recovery nozzle 5 for recovery. Take out the feed sample bag and allow the ether to evaporate to near dryness in a ventilated place.

[0054] Step 6: Place the preliminarily dried sample package in a constant temperature drying oven and adjust the temperature to (105±2)℃ for 2 hours, then place in a dryer to cool to room temperature (approximately 45-60 minutes). Place the feed sample package in a weighing bottle and weigh the sample package (c) to calculate the crude fat content.

[0055] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all these improvements and changes should fall within the scope of protection of the appended claims of the present invention.

Claims

1. A fat extraction tube, characterized in that: The invention comprises a fat extraction cylinder body with an upper opening, a water jacket layer is arranged on the outer ring of the fat extraction cylinder body, and a water inlet pipe and a water outlet pipe are connected to the water jacket layer; an extraction bottom bottle connecting end is arranged at the bottom end of the bottom of the fat extraction cylinder body; a siphon device is connected to the bottom of the fat extraction cylinder body, and is used for continuously returning the extraction solvent in the fat extraction cylinder body to the extraction bottom bottle connected to the extraction bottom bottle connecting end, wherein the siphon device comprises a first siphon tube connected to the bottom of the fat extraction cylinder body, and a spherical cavity structure connected to the first siphon tube. A second siphon is arranged on the spherical cavity structure, and the second siphon is connected to the extraction bottom bottle connecting end. A straight pressure relief pipe is arranged on the spherical cavity structure, and is connected to the upper part of the fat extraction cylinder body; a connecting pipe is arranged on the fat extraction cylinder body, one end of the connecting pipe is connected to the extraction bottom bottle connecting end, and the other end is connected to the upper end of the fat extraction cylinder body. The upper end opening of the fat extraction cylinder is a ground mouth, which is convenient for connecting to the condensation device; the extraction bottom bottle connecting end at the lower end of the fat extraction cylinder is a ground mouth, which is convenient for connecting to the extraction bottom bottle.

2. The fat extraction cartridge according to claim 1, characterized in that: A solvent recovery nozzle is arranged at the bottom of the fat extraction cylinder body, and a rubber tube and a spring clip matched with the rubber tube are installed on the solvent recovery nozzle.

3. Fat extractor, characterized in that: The invention comprises the fat extraction cylinder as claimed in claim 1, wherein the lower end of the fat extraction cylinder is connected to the extraction bottom bottle connecting end, and the upper end of the fat extraction cylinder is connected to the condensation device.

4. The method for using the fat extractor according to claim 3, characterized in that: The steps are as follows: placing the sample package to be tested into the body of the fat extraction cylinder, adding extraction solvent to submerge the sample to be extracted; Add extraction solvent to the extraction bottom bottle; connect the fat extraction cylinder, extraction bottom bottle and condensation device, and connect the condensation device to running tap water for condensation; Connect the water jacket layer arranged outside the fat extraction cylinder body to the water inlet and outlet of the circulating water heater respectively, adjust the water temperature of the circulating water heater, and keep the hot water circulating in the water jacket of the fat extraction cylinder; Place the extraction bottom bottle on a water bath and heat the water bath for extraction.

5. The method of use according to claim 4, characterized in that: Add glass balls or pumice stones to the extraction bottom bottle and then add the extraction solvent; add the extraction solvent to the fat extraction cylinder so that it exceeds the height of the sample package to be tested by 2cm.

6. The method of use according to claim 4, characterized in that: The sample package to be tested is an oil sample package; the extraction solvent is anhydrous ether, the water bath temperature is 60-70°C, so that the condensed ether drops in the form of beads; the circulating water temperature of the water jacket of the fat extraction cylinder is 50-60°C.

7. The method of use according to claim 4, characterized in that: After the extraction is completed, turn off the circulating water heater and the water bath power supply, and release the ether in the extraction cylinder device through the solvent recovery nozzle for recovery; take out the sample package to be tested, let the extraction solvent evaporate in a ventilated place, dry it in a drying oven and then weigh it, calculate the weight difference before and after the extraction of the sample package to be tested, and calculate the crude fat content.

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