Transfer valve and PCR chip

Abstract

The present invention discloses a rotary valve and a PCR chip, including a sealing member and a supporting member. The sealing member can undergo elastic deformation, and a connecting hole and a valve channel are provided on the sealing member. The supporting member is made of a hard material, and the supporting member includes a connecting portion and a supporting portion, the connecting portion is fixed to the supporting portion, and one end of the supporting portion away from the connecting portion is inserted into the connecting hole. The sealing member with a certain elasticity can effectively improve the sealing and improve the accuracy of the experiment when applied to the PCR chip. At the same time, the supporting member cooperates with the sealing member by plugging, and can provide effective support for the sealing member, ensuring that the rotary valve can be used normally. Therefore, in actual use, the rotary valve does not need to cooperate with other components, so that the rotary valve simplifies its own structure while ensuring good sealing.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic detection, and in particular to a rotary valve and a PCR chip. Background Art

[0002] In the related art, the rotary valve in the PCR chip is usually made of hard materials, and in actual use, the sealing performance is often poor. In order to improve the sealing performance, additional sealing rings are added, resulting in a complicated internal structure of the PCR chip. Summary of the invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a rotary valve that can ensure a compact structure while ensuring sealing performance.

[0004] The present invention also provides a PCR chip having the rotary valve.

[0005] A first aspect of the present invention provides a rotary valve, comprising:

[0006] A sealing member, the sealing member can be elastically deformed and is provided with a connecting hole and a valve channel;

[0007] The support member comprises a connecting portion and a supporting portion, wherein the connecting portion is fixed to the supporting portion, and an end of the supporting portion away from the connecting portion is inserted into the connecting hole.

[0008] The rotary valve according to the embodiment of the present invention has at least the following technical effects:

[0009] The sealing member with a certain elasticity can effectively improve the sealing performance and the accuracy of the experiment when applied to the PCR chip. At the same time, the support member cooperates with the sealing member by plugging, which can provide effective support for the sealing member and ensure the normal use of the rotary valve. Therefore, in actual use, the rotary valve does not need to cooperate with other components, so that the rotary valve can simplify its own structure while ensuring good sealing performance.

[0010] According to the rotary valve of some embodiments of the present invention, the support member further includes a torsion portion, the torsion portion is fixed to the connection portion, and a torsion groove is provided on the torsion portion.

[0011] According to the rotary valve of some embodiments of the present invention, an anti-foolproof groove is provided on the connecting portion, and the anti-foolproof groove is used to indicate the position of the connecting portion.

[0012] A second aspect of the present invention provides a PCR chip, comprising:

[0013] A chip body, wherein a processing chamber and an amplification chamber are arranged in the chip body, and a first containing groove, a second containing groove and an air outlet are arranged on the chip body, wherein the first containing groove is located between the processing chamber and the amplification chamber and is connected to the processing chamber and the amplification chamber, and the second containing groove is located between the amplification chamber and the air outlet and is connected to the amplification chamber and the air outlet;

[0014] Two rotary valves according to the first embodiment of the present invention, one rotary valve is arranged in the first receiving groove, and the other rotary valve is arranged in the second receiving groove, the rotary valve at the first receiving groove is the first rotary valve, and the rotary valve at the second receiving groove is the second rotary valve, the valve channel of the first rotary valve rotated to the first preset position connects the processing chamber with the amplification chamber, the valve channel of the second rotary valve rotated to the second preset position connects the amplification chamber with the gas outlet, the first rotary valve rotated away from the first preset position and the second rotary valve rotated away from the second preset position keep the amplification chamber sealed.

[0015] The PCR chip according to the embodiment of the present invention has at least the following technical effects:

[0016] By adopting the rotary valve, the sealing performance of the amplification chamber during the detection process is effectively optimized, and at the same time the structure of the PCR chip is simplified.

[0017] According to some embodiments of the PCR chip of the present invention, three rotary valves are provided, and the chip body is also provided with a first air channel, a second air channel and a plurality of independent feeding chambers, and the chip body is also provided with a third accommodating groove, and the third accommodating groove is located between the feeding chamber and the processing chamber, and the third accommodating groove is connected to the processing chamber and each of the feeding chambers, and a rotary valve is provided at the third accommodating groove, and the rotary valve at the third accommodating groove is the third rotary valve, and the valve channel of the third rotary valve rotated to a third preset position connects the feeding chamber with the processing chamber, the first air channel connects the processing chamber and the first accommodating groove, the second air channel connects the first accommodating groove and the air outlet, and the valve channel of the first rotary valve rotated to a fourth preset position connects the first air channel with the second air channel.

[0018] According to some embodiments of the present invention, the PCR chip further includes a negative pressure air pump, which is disposed at the air outlet.

[0019] According to some embodiments of the PCR chip of the present invention, a buffer cavity is further provided in the chip body, and the buffer cavity is located between the gas outlet and the second containing groove, and is connected to the gas outlet and the second containing groove.

[0020] According to some embodiments of the present invention, the PCR chip further includes a positive pressure air pump, and the chip body is also provided with an air blowing port, the air blowing port is connected to the second air channel, and the positive pressure air pump is arranged at the air blowing port.

[0021] According to some embodiments of the PCR chip of the present invention, a plurality of collection holes connected to the amplification chamber are further provided in the chip body, and the collection holes are independent of each other.

[0022] In the PCR chip according to some embodiments of the present invention, the diameter of the collection hole gradually decreases in a direction away from the amplification chamber.

[0023] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0025] Figure 1 It is a three-dimensional schematic diagram of a rotary valve in an embodiment of the present invention;

[0026] Figure 2 This is an exploded schematic diagram of a rotary valve in an embodiment of the present invention;

[0027] Figure 3 A three-dimensional schematic diagram of a PCR chip in an embodiment of the present invention;

[0028] Figure 4 It is a three-dimensional schematic diagram of the PCR chip in an embodiment of the present invention.

[0029] Figure markings: first rotary valve 101, second rotary valve 102, third rotary valve 103, sealing member 110, connecting hole 111, valve channel 112, supporting member 120, connecting portion 121, supporting portion 122, torsion portion 123, torsion groove 124, fool-proof groove 125, chip body 200, first accommodating groove 201, second accommodating groove 202, third accommodating groove 203, processing chamber 210, amplification chamber 220, collection hole 221, gas outlet 230, feeding chamber 240, liquid inlet 241, first air channel 250, second air channel 260, buffer chamber 270, EP tube 300. DETAILED DESCRIPTION

[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0031] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0032] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0033] Reference below Figure 1 and Figure 2 A rotary valve according to an embodiment of the present invention is described.

[0034] The rotary valve according to the first embodiment of the present invention includes a sealing member 110 and a supporting member 120 .

[0035] The seal 110 can be elastically deformed, and is provided with a connection hole 111 and a valve channel 112. The support 120 includes a connection portion 121 and a support portion 122, wherein the connection portion 121 is fixed to the support portion 122, and one end of the support portion 122 away from the connection portion 121 is inserted into the connection hole 111.

[0036] Specifically, the support member 120 includes a connecting portion 121 and at least one supporting portion 122. The connecting portion 121 and the supporting portion 122 are both cylindrical, one end surface of the supporting portion 122 is fixed to the bottom surface of the connecting portion 121, and the axis of the connecting portion 121 is parallel to the axis of the supporting portion 122. The support member 120 can be made of hard materials such as aluminum alloy and stainless steel by machining, integral injection molding or 3D printing, so it has a certain rigidity and strength.

[0037] The seal 110 is made of flexible materials such as PDMS or silicone, and is made by integral injection molding or 3D printing, so it has a certain elasticity. The seal 110 is in the shape of a truncated cone, a cylindrical shape, or a truncated cone and a cylindrical shape, and at least one connecting hole 111 is provided on the bottom surface of one side thereof. The number of connecting holes is the same as the number of supporting parts 122, and the positions of the two correspond one to one. The aperture of the connecting hole is slightly smaller than the diameter of the supporting part 122, so that the supporting part 122 can be inserted into the connecting hole with interference. Thus, the support 120 is fixed to the seal 110 by the mutual cooperation between the supporting part 122 and the connecting hole, and at this time, the axis of the connecting part 121 is colinear with the axis of the seal 110. When a torque in the axial direction is applied to the support 120, the support 120 can drive the seal 110 to rotate synchronously with the fixed axis.

[0038] Preferably, multiple support portions 122 and connecting holes 111 are provided, thereby ensuring that the support member 120 will not rotate relative to the sealing member 110, avoiding slippage between the two, and ensuring that the support member 120 can effectively drive the sealing member 110 to rotate synchronously.

[0039] Meanwhile, a valve channel 112 is provided inside the sealing member 110. The valve channel 112 has at least two openings on the side of the sealing member 110, that is, when the rotary valve is a two-way valve, the valve channel 112 has two openings on the side of the sealing member 110; when the rotary valve is a three-way valve, the valve channel 112 has three or more openings on the side of the sealing member 110, and the number of openings will change adaptively with the change of the rotary valve type.

[0040] In the specific use process, the rotary valve installed on the chip body 200 of the PCR chip will contact the chip body 200 of the PCR chip through the sealing member 110. It is not difficult to understand that the sealing member 110 with a certain elasticity will fit tightly on the groove wall of the accommodating groove set on the chip body 200, thereby ensuring the sealing of the rotary valve. When the sealing member 110 needs to rotate to change the object connected to the valve channel 112, a torque is applied to the support member 120, and the support member 120 is used to drive the sealing member 110 to rotate. The plug-in cooperation between the support member 120 and the sealing member 110 also effectively simplifies the structure of the rotary valve.

[0041] In summary, such a design simplifies the specific structure of the rotary valve while ensuring good sealing performance of the rotary valve.

[0042] In some specific embodiments of the present invention, the support member 120 further includes a torsion portion 123 , which is fixed to the connection portion 121 , and a torsion groove 124 is disposed on the torsion portion 123 .

[0043] Specifically, the support member 120 also includes a torsion portion 123, and the function of the torsion portion 123 is to cooperate with the output end of the driving structure so that the driving member can drive the connection portion 121 to drive the support portion 122 to rotate. The torsion portion 123 is fixed on the bottom surface of the connection portion 121 away from the support portion 122, and is also cylindrical. A torsion groove 124 is provided on the bottom surface of the torsion portion 123 on one side away from the connection portion 121. The torsion groove 124 can be in a "cross" shape or a "one" shape, and its depth, length and width are all compatible with the output end of the driving structure, so that the output end of the driving structure can be embedded in the torsion groove 124, and the torque output to the connection portion 121 is achieved by applying a force to the groove wall of the torsion groove 124. The driving structure can be a rotary cylinder or a rotary motor, etc.

[0044] It can be understood that the setting of the torsion portion 123 and the torsion groove 124 arranged thereon enables the driving structure to indirectly apply torque to the supporting portion 122, and the working state of the driving structure can be preset in advance, so that the rotation angle of the rotary valve can be automatically controlled, thereby improving the degree of automation.

[0045] In some specific embodiments of the present invention, an anti-mistake groove 125 is provided on the connecting portion 121 , and the anti-mistake groove 125 is used to indicate the position of the connecting portion 121 .

[0046] Specifically, because the opening of the valve channel 112 disposed on the side of the sealing member 110 needs to be connected to the corresponding cavity, the installation direction of the rotary valve needs to be confirmed when the rotary valve is installed on the chip body 200 of the PCR chip. To improve assembly positioning, an anti-mistake groove 125 is provided on the connecting portion 121.

[0047] The foolproof groove 125 is arranged on the side of the connecting portion 121 away from the supporting portion 122 and is located at the edge of the connecting portion 121 so that the installer can easily find it. The foolproof groove 125 is used to assist in positioning. By providing the foolproof groove 125, the staff can quickly identify the correct installation direction when installing the rotary valve at the corresponding position on the chip body 200, thereby improving the installation efficiency.

[0048] Reference below Figure 3 and Figure 4 A PCR chip according to an embodiment of the present invention is described.

[0049] The PCR chip according to the second embodiment of the present invention comprises a chip body 200 and two rotary valves according to the first embodiment of the present invention.

[0050] Among them, a processing chamber 210 and an amplification chamber 220 are arranged in the chip body 200, and a first accommodating groove 201, a second accommodating groove 202 and an air outlet 230 are arranged on the chip body. The first accommodating groove 201 is located between the processing chamber 210 and the amplification chamber 220, and is connected to the processing chamber 210 and the amplification chamber 220. The second accommodating groove 202 is located between the amplification chamber 220 and the air outlet 230, and is connected to the amplification chamber 220 and the air outlet 230. One rotary valve is arranged in the first receiving groove 201, and the other rotary valve is arranged in the second receiving groove 202. The rotary valve at the first receiving groove 201 is the first rotary valve 101, and the rotary valve at the second receiving groove 202 is the second rotary valve 102. The valve channel 112 of the first rotary valve 101 rotated to the first preset position connects the processing chamber 210 with the amplification chamber 220. The valve channel 112 of the second rotary valve 102 rotated to the second preset position connects the amplification chamber 220 with the gas outlet 230. The first rotary valve 101 rotated away from the first preset position and the second rotary valve 102 rotated away from the second preset position keep the amplification chamber 220 sealed.

[0051] Specifically, the chip body 200 is in the shape of a strip plate, and is made of hard plastic materials such as PP and PC, and is provided with a processing chamber 210 and an amplification chamber 220, and a first receiving groove 201, a second receiving groove 202 and an air outlet 230 are sequentially provided on a side with a larger area, wherein the first receiving groove 201 is located between the processing chamber 210 and the amplification chamber 220, and the second receiving groove 202 is located between the air outlet 230 and the amplification chamber 220. In addition, many micro-channels are also provided in the chip body 200, and the processing chamber 210, the first receiving groove 201, the amplification chamber 220, the second receiving groove 202 and the air outlet 230 are sequentially connected through the micro-channels.

[0052] A rotary valve is disposed in each of the first receiving groove 201 and the second receiving groove 202. For the convenience of distinction, the rotary valve installed in the first receiving groove 201 is named the first rotary valve 101, and the rotary valve installed in the second receiving groove 202 is named the second rotary valve 102. The sealing member 110 in the first rotary valve 101 is completely accommodated in the first receiving groove 201. By applying torque to the supporting member 120 in the first rotary valve 101, the sealing member 110 in the first receiving groove 201 can be driven to rotate synchronously. During the rotation process, the position of the opening of the valve channel 112 disposed on the sealing member 110 will change, and the position of the first rotary valve 101 when the processing chamber 210 and the amplification chamber 220 can be connected through the valve channel 112 is set to the first preset position.

[0053] Correspondingly, the sealing member 110 in the second rotary valve 102 will also be completely accommodated in the second accommodating groove 202. By applying torque to the supporting member 120 in the second rotary valve 102, the sealing member 110 in the second accommodating groove 202 can be driven to rotate synchronously. During the rotation process, the position of the opening of the valve channel 112 provided on the sealing member 110 will change, and the position of the second rotary valve 102 when the air outlet 230 and the amplification chamber 220 can be connected through the valve channel 112 is set to the second preset position.

[0054] A collection hole 221 is provided on the inner wall of the amplification chamber 220 , and a microcentrifuge tube (EP tube 300 ) is provided below the collection hole 221 . When the liquid to be tested in the processing chamber 210 flows to the collection hole 221 , the liquid to be tested will flow into the EP tube 300 .

[0055] In the specific use process, first, the test liquid to be tested is input into the processing chamber 210, and then the first rotary valve 101 is rotated to the first preset position, and the second rotary valve 102 is rotated to the second preset position. At this time, the processing chamber 210, the amplification chamber 220 and the gas outlet 230 are connected through the valve channel 112 of the first rotary valve 101 and the valve channel 112 of the second rotary valve 102. Afterwards, the test liquid in the processing chamber 210 flows to the amplification chamber 220 through the valve channel 112 of the first rotary valve 101 under the action of its own gravity or the action of the negative pressure valve set at the gas outlet 230. In this process, the air in the amplification chamber 220 is squeezed out from the gas outlet 230. When the test liquid flowing into the amplification chamber 220 flows to the collection hole 221, it will fall into the EP tube 300. When there is enough liquid to be tested in the EP tube 300 , the first rotary valve 101 and the second rotary valve 102 are rotated respectively, so that the amplification chamber 220 is no longer connected to the gas outlet 230 and the processing chamber 210 , thereby achieving sealing of the amplification chamber 220 .

[0056] It can be understood that, by adopting the above rotary valve, the sealing performance of the amplification chamber 220 during the detection process is effectively optimized, and at the same time the structure of the PCR chip is simplified.

[0057] In some specific embodiments of the present invention, three rotary valves are provided, a first gas channel 250, a second gas channel 260 and a plurality of mutually independent feeding chambers 240 are further provided in the chip body 200, a third accommodating groove 203 is further provided on the chip body 200, the third accommodating groove 203 is located between the feeding chamber 240 and the processing chamber 210, the third accommodating groove 203 is connected to the processing chamber 210 and each feeding chamber 240, a rotary valve is provided at the third accommodating groove 203, and the third gas channel 250, the second gas channel 260 and the plurality of mutually independent feeding chambers 240 are further provided in the chip body 200, and the third accommodating groove 203 is located between the feeding chamber 240 and the processing chamber 210, and the third accommodating groove 203 is connected to the processing chamber 210 and each feeding chamber 240, and a rotary valve is provided at the third accommodating groove 203. The rotary valve at the accommodating groove 203 is the third rotary valve 103. The valve channel 112 of the third rotary valve 103 rotated to the third preset position connects the feeding chamber 240 with the processing chamber 210, the first air channel 250 connects the processing chamber 210 and the first accommodating groove 201, the second air channel 260 connects the first accommodating groove 201 with the air outlet 230, and the valve channel 112 of the first rotary valve 101 rotated to the fourth preset position connects the first air channel 250 with the second air channel 260.

[0058] Specifically, a plurality of feeding chambers 240 are further provided in the chip body 200, and the plurality of feeding chambers 240 are independent of each other, that is, the feeding chambers 240 are not directly connected. Buffer, magnetic bead solution, ethanol solution, nucleic acid eluent or nucleic acid amplification premix, etc. are respectively provided in each feeding chamber 240. At this time, the feeding chamber 240, the processing chamber 210 and the amplification chamber 220 are arranged in sequence. The chip body 200 is further provided with a third containing groove 203 between each feeding chamber 240 and the processing chamber 210, and the third containing groove 203 is respectively connected with each feeding chamber 240 and the processing chamber 210 through micro-channels.

[0059] At the same time, a liquid inlet 241 corresponding to the feeding cavity 240 is also provided on the chip body 200 , and the corresponding liquid in the feeding cavity 240 is input through the liquid inlet 241 .

[0060] The PCR chip is also provided with a third rotary valve, which is installed in the third receiving groove 203. For the convenience of distinction, the rotary valve in the third receiving groove 203 is named as the third rotary valve 103. At this time, the sealing member 110 of the third rotary valve 103 is completely accommodated in the third receiving groove 203. By applying torque to the support member 120 in the third rotary valve 103, the sealing member 110 in the third receiving groove 203 can be driven to rotate synchronously. During the rotation process, the position of the opening of the valve channel 112 provided on the sealing member 110 will change, and the position of the third rotary valve 103 when the feeding chamber 240 and the amplification chamber 220 can be connected through the valve channel 112 is set as the third preset position. It can be understood that, since a plurality of independent feeding chambers 240 are provided, and there is only one feeding chamber 240 connected to the processing chamber 210 through the valve channel 112 each time, the third preset position is a general reference, that is, in the actual operation process, the third preset position includes sub-positions equal in number to the number of feeding chambers 240.

[0061] In addition, the chip body 200 is also provided with a first air channel 250 and a second air channel 260, and the function of the first air channel 250 and the second air channel 260 is to discharge the air in the processing chamber 210 directly from the air outlet 230 bypassing the amplification chamber 220. Therefore, the first air channel 250 connects the processing chamber 210 with the first accommodating groove 201, and the second air channel 260 connects the first accommodating groove 201 with the air outlet 230. At this time, when the valve channel 112 in the first rotary valve 101 connects the first air channel 250 with the second air channel 260, the position of the first rotary valve 101 is set to the fourth preset position. It is not difficult to understand that the first rotary valve 101 at the fourth preset position will block the connection between the processing chamber 210 and the amplification chamber 220, and the first rotary valve 101 at the first preset position will also block the connection between the first air channel 250 and the second air channel 260.

[0062] In the specific use process, first, the corresponding liquid is input into each feeding chamber 240 through the liquid inlet 241, and then the feeding chamber 240 where the liquid to be added to the processing chamber 210 in advance is located is selected, and then the third rotary valve 103 is rotated to the sub-position corresponding to the feeding chamber 240 in the third preset position, so that the feeding chamber 240 is connected with the processing chamber 210. At this time, the first rotary valve 101 is rotated to the fourth preset position, so that the first air channel 250 is connected with the second air channel 260, and then, under the self-gravity of the liquid in the feeding chamber 240 or the negative pressure air valve set at the air outlet 230, the liquid flows into the processing chamber 210. In this process, the air in the processing chamber 210 is discharged from the air outlet 230 through the first air channel 250, the valve channel 112 of the first rotary valve 101 and the second air channel 260.

[0063] Similarly, when the liquid in the other feeding chamber 240 needs to be added to the processing chamber 210, the third rotary valve 103 only needs to be rotated to the corresponding sub-position in the third preset position. The liquid in the processing chamber 210 becomes the liquid to be tested after pretreatment such as mixing or heating.

[0064] Finally, the first rotary valve 101 is rotated to the first preset position, and as mentioned above, the liquid to be tested in the processing chamber 210 is transferred to the amplification chamber 220 and finally falls into the EP tube 300 .

[0065] It can be understood that such a design allows multiple different types of liquids to be added at one time, and these liquids can mix themselves in the processing chamber 210, and finally combine into the liquid to be tested, and then enter the amplification chamber 220 and complete the collection by the corresponding EP tube 300, which effectively improves the detection efficiency.

[0066] In some specific embodiments of the present invention, a negative pressure air pump is further included, and the negative pressure air pump is disposed at the air outlet 230 .

[0067] Specifically, a negative pressure air pump is provided at the air outlet 230. The function of the negative pressure air pump is to extract the air in the processing chamber 210 and / or the amplification chamber 220, so as to form a negative pressure in the processing chamber 210 and / or the amplification chamber 220, thereby accelerating the flow speed of the liquid in the processing chamber 210 and / or the amplification chamber 220, thereby improving the detection efficiency.

[0068] In some specific embodiments of the present invention, a buffer cavity 270 is further disposed in the chip body 200 . The buffer cavity 270 is located between the gas outlet 230 and the second receiving groove 202 , and is communicated with the gas outlet 230 and the second receiving groove 202 .

[0069] Specifically, the chip body 200 is further provided with a buffer chamber 270 between the gas outlet 230 and the second containing groove 202, and the buffer chamber 270 is respectively connected with the gas outlet 230 and the second containing groove 202 through a microchannel. In actual use, when the second rotary valve 102 is located at the second preset position, the negative pressure air pump will extract the air in the amplification chamber 220, so that a negative pressure is formed in the amplification chamber 220, thereby inducing the liquid to be tested to flow in the amplification chamber 220.

[0070] It is understandable that in this process, it is inevitable that some of the liquid to be tested will pass through the valve channel 112 in the second rotary valve 102, flow out of the amplification chamber 220 and flow toward the direction of the gas outlet 230. The setting of the buffer chamber 270 enables this part of the liquid to be tested to be effectively buffered, thereby preventing the liquid to be tested from flowing into the gas outlet 230 and causing the gas outlet 230 to be blocked or flowing to the outside of the chip body 200.

[0071] In some specific embodiments of the present invention, a positive pressure air pump is further included, and an air blowing port is also provided on the chip body 200, the air blowing port is connected to the second air channel 260, and the positive pressure air pump is provided at the air blowing port.

[0072] Specifically, the chip body 200 is also provided with an air port connected to the second air channel 260, and a positive pressure air pump is provided at the air port. When a sufficient amount of the liquid to be tested has been collected in the EP tube 300, it is not necessary to continue to maintain the sealing of the amplification chamber 220. At this time, the first rotary valve 101 is rotated to the fourth preset position, so that the first air channel 250 is connected to the second air channel 260. At this time, the positive pressure air pump blows air toward the second air channel 260 through the air port, and the airflow is finally blown into the processing chamber 210, and then the residual liquid in the processing chamber 210 is blown back to the feeding chamber 240 connected to the valve channel 112 in the third rotary valve 103 rotated to the third preset position.

[0073] It is understandable that such a design eliminates the need to clean the processing chamber 210 at a later stage, thereby reducing the number of cleaning steps and improving cleaning efficiency.

[0074] As an improvement of the above solution, the positive pressure air pump and the negative pressure air pump are the same air pump, which can perform the functions of suction and blowing at different times, and the air outlet 230 and the air blowing port are the same opening. It can be understood that such a design simplifies the structure of the PCR chip.

[0075] In some specific embodiments of the present invention, a plurality of collection holes 221 communicating with the amplification chamber 220 are further disposed in the chip body 200 , and each collection hole 221 is independent of each other.

[0076] Specifically, a plurality of collection holes 221 are provided on the inner wall of the amplification chamber 220, and each collection hole 221 is independent of each other and is respectively connected to the first accommodating tank 201 and the second accommodating tank 202 through microchannels that are not directly connected. It can be understood that at this time, the first preset position also includes a plurality of sub-positions, and the valve channels 112 of the first rotary valve 101 at different sub-positions are connected to different collection holes 221. According to the different liquids added to the processing chamber 210, the mixed liquids can be successively made to flow into the amplification chamber 220 through the valve channels 112 of the first rotary valve 101 at the respective corresponding sub-positions, and flow into the desired EP tube 300 through the desired collection holes 221. Such a design enables the PCR chip to perform "single sample multi-index" detection.

[0077] In some specific embodiments of the present invention, the diameter of the collection hole 221 gradually decreases in a direction away from the amplification chamber 220 .

[0078] Specifically, the collection hole 221 is in a truncated cone shape, and its aperture gradually decreases in the direction away from the amplification chamber 220. It can be understood that under such a design, the liquid flowing into the EP tube 300 through the collection hole 221 will be guided by the side wall of the collection hole 221 with a certain inclination during the flow process, and the large diameter of the contact between the collection hole 221 and the amplification chamber 220 also accelerates the inflow speed of the liquid to be tested, thereby improving the detection efficiency.

[0079] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. PCR chip, characterized in that, include: A chip body, wherein a processing chamber and an amplification chamber are arranged in the chip body, and a first containing groove, a second containing groove and an air outlet are arranged on the chip body, wherein the first containing groove is located between the processing chamber and the amplification chamber and is connected to the processing chamber and the amplification chamber, and the second containing groove is located between the amplification chamber and the air outlet and is connected to the amplification chamber and the air outlet; At least two rotary valves, the rotary valves include a sealing member and a supporting member, the sealing member can be elastically deformed, a connecting hole and a valve channel are arranged on the sealing member, the supporting member includes a connecting portion and a supporting portion, the connecting portion is fixed to the supporting portion, and one end of the supporting portion away from the connecting portion is inserted into the connecting hole, the supporting member also includes a twisting portion, the twisting portion is fixed to the connecting portion, a twisting groove is arranged on the twisting portion, and an anti-idiot groove is arranged on the connecting portion, the anti-idiot groove is used to indicate the position of the connecting portion, one rotary valve is arranged in the first receiving groove, and the other rotary valve is arranged in the second receiving groove, the rotary valve at the first receiving groove is the first rotary valve, and the rotary valve at the second receiving groove is the second rotary valve, the valve channel of the first rotary valve rotated to the first preset position connects the processing chamber with the amplification chamber, and the valve channel of the second rotary valve rotated to the second preset position connects the amplification chamber with the gas outlet The first rotary valve rotated away from the first preset position and the second rotary valve rotated away from the second preset position keep the amplification chamber sealed, the PCR chip includes three rotary valves, the chip body is also provided with a first air channel, a second air channel and a plurality of independent feeding chambers, the chip body is also provided with a third accommodating groove, the third accommodating groove is located between the feeding chamber and the processing chamber, the third accommodating groove is connected to the processing chamber and each of the feeding chambers, a rotary valve is arranged at the third accommodating groove, the rotary valve at the third accommodating groove is the third rotary valve, the valve channel of the third rotary valve rotated to the third preset position makes the feeding chamber connected with the processing chamber, the first air channel is connected to the processing chamber and the first accommodating groove, the second air channel is connected to the first accommodating groove and the air outlet, the valve channel of the first rotary valve rotated to the fourth preset position makes the first air channel connected with the second air channel.

2. The PCR chip according to claim 1, characterized in that: It also includes a negative pressure air pump, which is arranged at the air outlet.

3. The PCR chip according to claim 2, characterized in that: A buffer cavity is also provided in the chip body, and the buffer cavity is located between the air outlet and the second containing groove, and is communicated with the air outlet and the second containing groove.

4. The PCR chip according to claim 1, characterized in that: It also includes a positive pressure air pump. The chip body is also provided with an air blowing port, the air blowing port is connected to the second air channel, and the positive pressure air pump is arranged at the air blowing port.

5. The PCR chip according to claim 1, characterized in that: The chip body is also provided with a plurality of collection holes connected with the amplification chamber, and each of the collection holes is independent of each other.

6. The PCR chip according to claim 5, characterized in that: The diameter of the collection hole gradually decreases in a direction away from the amplification chamber.

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