Bioreactor exhaust pipe, bioreactor and exhaust pipe control method

Abstract

The embodiment of the present invention provides a bioreactor exhaust pipeline, a bioreactor and an exhaust pipeline control method, wherein the bioreactor exhaust pipeline includes a controller, a first pipeline and a second pipeline; the first pipeline and the second pipeline both include a sterilization pipeline and a filter pipeline; one end of the filter pipeline is used to connect to the reactor tank body, and the other end is used to connect to the exhaust port; one end of the sterilization pipeline is used to connect to the steam outlet, and the other end is used to connect to the sewage outlet; the controller is used to open the filter pipeline of the current standby pipeline, close the filter pipeline of the working pipeline, and open the sterilization pipeline of the working pipeline when the pressure in the reactor tank body is greater than or equal to a preset threshold, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline. The embodiment of the present invention solves the problem that bacteria in the air reversely contaminate the cell culture fluid in the reactor through the exhaust pipeline.

Description

Technical Field

[0001] The embodiments of the present invention relate to the field of bioreactors, and in particular to a bioreactor exhaust pipe, a bioreactor, and an exhaust pipe control method. Background Art

[0002] Normal cell culture temperature is generally 27-37℃, and the culture cycle is generally 3-30 days. Therefore, some cell culture processes require a culture cycle of 37℃ and 30 days. When the tank is ventilated continuously at a deep level, the exhaust gas often contains a lot of water vapor. Because the filter needs to be equipped with a hydrophobic filter element that can pass air but not water, the filter element in the filter is often blocked by water vapor in the exhaust gas, resulting in failure to vent normally, resulting in the problem of rising pressure in the tank, and leading to cell culture failure. Summary of the invention

[0003] The embodiment of the present invention provides a bioreactor exhaust duct, a bioreactor and an exhaust duct control method to solve the problem that the filter element in the filter of the existing bioreactor is often blocked by water vapor in the exhaust gas, resulting in failure of normal exhaust and increased pressure in the tank, leading to cell culture failure.

[0004] In order to solve the above-mentioned technical problems, the present invention is achieved as follows:

[0005] In a first aspect, an embodiment of the present invention provides a bioreactor exhaust pipeline, including a controller, a first pipeline and a second pipeline;

[0006] The first pipeline and the second pipeline both include a sterilization pipeline and a filtration pipeline;

[0007] One end of the filter pipeline is used to connect to the reactor tank, and the other end is used to connect to the exhaust port;

[0008] One end of the sterilization pipeline is used to be connected to the steam outlet, and the other end is used to be connected to the sewage outlet;

[0009] The controller is used to open the filter pipeline of the current standby pipeline, close the filter pipeline of the working pipeline, and open the sterilization pipeline of the working pipeline when the pressure in the reactor tank is greater than or equal to a preset threshold value, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

[0010] Optionally, the filtering pipeline includes a filter, a first pipeline, a second pipeline, a first valve and a second valve;

[0011] The first end of the filter is connected to the reactor tank through the first pipeline, and the first valve is arranged on the first pipeline;

[0012] The second end of the filter is connected to the exhaust port through the second pipe, and the second valve is arranged on the second pipe;

[0013] The controller opens the filter line by controlling the opening of the first valve and the second valve.

[0014] Optionally, the sterilization pipeline includes a third pipeline, a fourth pipeline, a first temperature electrode, a first steam trap, a second temperature electrode, a second steam trap, a third valve, a fourth valve and a fifth valve;

[0015] The third valve is disposed between the second valve and the first end of the filter, and is used to connect to the steam outlet and control the opening or closing of the steam;

[0016] One end of the third pipe is connected to the second end of the filter, and the other end is connected to the sewage outlet;

[0017] The fourth valve is arranged on the third pipeline, one end of which is connected to the second end of the filter, and the other end of which is connected to one end of the first temperature electrode;

[0018] One end of the first steam trap is connected to the other end of the first temperature electrode, and the other end is connected to the sewage outlet;

[0019] One end of the fourth pipe is connected to the first end of the filter, and the other end is connected to the sewage outlet;

[0020] The fifth valve is arranged on the fourth pipeline, one end of which is connected to the first end of the filter, and the other end of which is connected to one end of the second temperature electrode;

[0021] One end of the second steam trap is connected to the other end of the second temperature electrode, and the other end is connected to the sewage outlet;

[0022] The controller opens the sterilization line by controlling the opening of the third valve, the fourth valve, and the fifth valve.

[0023] Optionally, an operating temperature of the first temperature electrode or the second temperature electrode is greater than or equal to 121 degrees Celsius.

[0024] Optionally, the filter includes a filter housing and a filter element, the filter housing is fixed on the filter pipeline, and the filter element is fixed in the filter housing.

[0025] In a second aspect, an embodiment of the present invention further provides a bioreactor, comprising a reactor tank body and a bioreactor exhaust pipe as described in any one of the first aspects connected to one end of the reactor tank body.

[0026] Optionally, the reactor tank is provided with a pressure sensor for detecting the pressure in the exhaust pipe.

[0027] In a third aspect, an embodiment of the present invention further provides an exhaust duct control method, which is applied to the bioreactor as described in the second aspect, comprising:

[0028] When the pressure in the reactor tank is greater than or equal to a preset threshold, the filter pipeline of the current standby pipeline is opened, the filter pipeline of the working pipeline is closed, and the sterilization pipeline of the working pipeline is opened, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

[0029] Optionally, after the sterilization pipeline of the working pipeline is opened for a preset time, the method further includes closing the sterilization pipeline.

[0030] In the embodiment of the present invention, the exhaust pipe of the bioreactor is used in a backup mode. When an abnormal situation occurs, such as filter element damage or filter element damage caused by excessive foam, the backup exhaust pipe can be used for processing. The new filter element can also be replaced in time and sterilized separately. The setting is simple, safe and reliable, and the exhaust is uninterrupted, which can maximize the cell culture effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present invention. Moreover, the same reference symbols are used throughout the accompanying drawings to represent the same components. In the accompanying drawings:

[0032] Figure 1 A schematic diagram of the structure of an exhaust pipe of a bioreactor provided in Example 1 of the present invention;

[0033] Figure 2 A schematic flow chart of an exhaust duct control method provided in Embodiment 2 of the present invention. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Please refer to Figure 1 , Figure 1This is a schematic diagram of the structure of a bioreactor exhaust pipeline provided in Example 1 of the present invention; the present invention provides a bioreactor exhaust pipeline, including: a controller, a first pipeline and a second pipeline;

[0036] The first pipeline and the second pipeline both include a sterilization pipeline and a filtration pipeline;

[0037] One end of the filter pipeline is used to connect to the reactor tank, and the other end is used to connect to the exhaust port;

[0038] One end of the sterilization pipeline is used to be connected to the steam outlet, and the other end is used to be connected to the sewage outlet;

[0039] The controller is used to open the filter pipeline of the current standby pipeline, close the filter pipeline of the working pipeline, and open the sterilization pipeline of the working pipeline when the pressure in the reactor tank is greater than or equal to a preset threshold value, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

[0040] In the embodiment of the present invention, the exhaust pipe of the bioreactor is used in a backup mode. When an abnormal situation occurs, such as filter element damage or filter element damage caused by excessive foam, the backup exhaust pipe can be used for processing. The new filter element can also be replaced in time and sterilized separately. The setting is simple, safe and reliable, and the exhaust is uninterrupted, which can maximize the cell culture effect.

[0041] In some embodiments of the present invention, optionally, the filtering pipeline includes a filter, a first pipeline, a second pipeline, a first valve V1 and a second valve V2;

[0042] The first end of the filter is connected to the reactor tank through the first pipeline, and the first valve V1 is arranged on the first pipeline;

[0043] The second end of the filter is connected to the exhaust port through the second pipe, and the second valve V2 is arranged on the second pipe;

[0044] The controller opens the filtering pipeline by controlling the first valve V1 and the second valve V2 to open.

[0045] In the embodiment of the present invention, the filter pipeline structure of the exhaust pipeline of the bioreactor is simple and the control method is easy to implement.

[0046] In some embodiments of the present invention, optionally, the sterilization pipeline includes a third pipeline, a fourth pipeline, a first temperature electrode TE1, a first steam trap S1, a second temperature electrode TE2, a second steam trap S2, a third valve V3, a fourth valve V4 and a fifth valve V5;

[0047] The third valve V3 is disposed between the second valve V2 and the first end of the filter, and is used to connect to the steam outlet and control the opening or closing of the steam;

[0048] One end of the third pipe is connected to the second end of the filter, and the other end is connected to the sewage outlet;

[0049] The fourth valve V4 is arranged on the third pipeline, one end of which is connected to the second end of the filter, and the other end of which is connected to one end of the first temperature electrode TE1;

[0050] One end of the first steam trap S1 is connected to the other end of the first temperature electrode TE1, and the other end is connected to the drain outlet;

[0051] One end of the fourth pipe is connected to the first end of the filter, and the other end is connected to the sewage outlet;

[0052] The fifth valve V5 is disposed on the fourth pipeline, one end of which is connected to the first end of the filter, and the other end of which is connected to one end of the second temperature electrode TE2;

[0053] One end of the second steam trap S2 is connected to the other end of the second temperature electrode TE2, and the other end is connected to the sewage outlet;

[0054] The controller opens the sterilization line by controlling the third valve V3, the fourth valve V4 and the fifth valve V5 to open.

[0055] In some embodiments of the present invention, optionally, when the second valve is a three-way valve, the first end is connected to the reactor tank, the second end is connected to the first end of the filter, the third end is connected to the second temperature electrode through a fourth pipe, and the fifth valve is arranged between the third end and the second temperature electrode.

[0056] In an embodiment of the present invention, one end of the sterilization pipeline of the bioreactor exhaust pipeline is connected to the steam outlet, and the other end is connected to the sewage outlet. When the temperature electrode of the sterilization pipeline reaches a certain temperature, the sterilization pipeline works normally; at this time, the steam flows to the first temperature electrode and the first steam trap through the fourth valve, and the first steam trap ensures that steam but not water passes through the pipeline, so that the water in the third pipeline is smoothly discharged to the sewage outlet; the steam flows to the second temperature electrode and the second steam trap through the filter and the fifth valve, which not only completes the sterilization control of the filter, but also realizes the drainage and sewage discharge of the pipeline.

[0057] In some embodiments of the present invention, optionally, the operating temperature of the first temperature electrode TE1 or the second temperature electrode TE2 is greater than or equal to 121 degrees Celsius.

[0058] In the embodiment of the present invention, when the temperature of the temperature electrode reaches 121 degrees Celsius, the sterilization pipeline starts to work normally.

[0059] In some embodiments of the present invention, optionally, the filter includes a filter housing and a filter element, the filter housing is fixed on the filter pipeline, and the filter element is fixed in the filter housing.

[0060] In the embodiment of the present invention, the filter is composed of a filter housing and a filter element, and has a simple structure and low cost.

[0061] The present invention further provides a bioreactor, comprising a reactor tank body and a bioreactor exhaust pipe as described in any of the above embodiments connected to one end of the reactor tank body.

[0062] In the embodiment of the present invention, the bioreactor is equipped with a backup bioreactor exhaust pipe. When an abnormal situation occurs, such as filter element damage or filter element damage caused by excessive foam, the backup exhaust pipe can be used to deal with it. The new filter element can also be replaced in time and sterilized separately, and the exhaust is uninterrupted, which can maximize the cell culture effect.

[0063] In some embodiments of the present invention, optionally, the reactor tank is provided with a pressure sensor for detecting the pressure in the exhaust pipe.

[0064] In some embodiments of the present invention, optionally, when the pressure in the pipeline is greater than or equal to 0.58 bar, the filter line of the current standby pipeline is opened, the filter line of the working pipeline is closed, and the sterilization line of the working pipeline is opened.

[0065] In an embodiment of the present invention, a pressure sensor is provided on the reactor tank body to detect the pressure in the exhaust pipe. When the filter in the exhaust pipe is clogged, the pressure in the exhaust pipe rises to a certain threshold within a certain period of time, triggering the controller to switch the exhaust pipeline to the backup pipeline.

[0066] See also Figure 2 , Figure 2 A schematic flow chart of an exhaust duct control method provided in Embodiment 2 of the present invention; the present invention further provides an exhaust duct control method, which is applied to a bioreactor as described in any of the above embodiments, comprising:

[0067] When the pressure in the reactor tank is greater than or equal to a preset threshold, the filter pipeline of the current standby pipeline is opened, the filter pipeline of the working pipeline is closed, and the sterilization pipeline of the working pipeline is opened, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

[0068] In an embodiment of the present invention, when the pressure of the bioreactor exhaust pipeline in the reactor tank is greater than or equal to a preset threshold value, the filter pipeline of the current standby pipeline is opened, the filter pipeline of the working pipeline is closed, and the sterilization pipeline of the working pipeline is opened, wherein, when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline. The control method is simple, which ensures uninterrupted exhaust of the bioreactor and can maximize the cell culture effect.

[0069] In some embodiments of the present invention, optionally, after the sterilization pipeline of the working pipeline is opened for a preset time, the method further includes closing the sterilization pipeline.

[0070] In some embodiments of the present invention, the preset time is 30-40 minutes.

[0071] In the embodiment of the present invention, the sterilization pipeline can be closed after working normally for a certain period of time, which ensures the sterilization effect and saves energy.

[0072] The embodiments of the present invention are described above in conjunction with the accompanying drawings, but the present invention is not limited to the above-mentioned specific implementation modes, which are merely illustrative rather than restrictive. Under the guidance of the present invention, ordinary technicians in this field can also make many forms without departing from the scope of protection of the present invention and the claims, all of which are within the protection of the present invention.

Claims

1. A bioreactor exhaust pipe, characterized in that: It includes a controller, a first pipeline and a second pipeline; The first pipeline and the second pipeline both include a sterilization pipeline and a filtration pipeline; One end of the filter pipeline is used to connect to the reactor tank, and the other end is used to connect to the exhaust port; One end of the sterilization pipeline is used to be connected to the steam outlet, and the other end is used to be connected to the sewage outlet; The controller is used to open the filter pipeline of the current standby pipeline, close the filter pipeline of the working pipeline, and open the sterilization pipeline of the working pipeline when the pressure in the reactor tank is greater than or equal to a preset threshold value, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

2. The bioreactor exhaust pipe according to claim 1, characterized in that: The filtering pipeline comprises a filter, a first pipeline, a second pipeline, a first valve and a second valve; The first end of the filter is connected to the reactor tank through the first pipeline, and the first valve is arranged on the first pipeline; The second end of the filter is connected to the exhaust port through the second pipe, and the second valve is arranged on the second pipe; The controller opens the filter line by controlling the opening of the first valve and the second valve.

3. The bioreactor exhaust pipe according to claim 2, characterized in that: The sterilization pipeline includes a third pipeline, a fourth pipeline, a first temperature electrode, a first steam trap, a second temperature electrode, a second steam trap, a third valve, a fourth valve and a fifth valve; The third valve is disposed between the second valve and the first end of the filter, and is used to connect to the steam outlet and control the opening or closing of the steam; One end of the third pipe is connected to the second end of the filter, and the other end is connected to the sewage outlet; The fourth valve is arranged on the third pipeline, one end of which is connected to the second end of the filter, and the other end of which is connected to one end of the first temperature electrode; One end of the first steam trap is connected to the other end of the first temperature electrode, and the other end is connected to the sewage outlet; One end of the fourth pipe is connected to the first end of the filter, and the other end is connected to the sewage outlet; The fifth valve is arranged on the fourth pipeline, one end of which is connected to the first end of the filter, and the other end of which is connected to one end of the second temperature electrode; One end of the second steam trap is connected to the other end of the second temperature electrode, and the other end is connected to the sewage outlet; The controller opens the sterilization line by controlling the opening of the third valve, the fourth valve, and the fifth valve.

4. The bioreactor exhaust pipe according to claim 3, characterized in that: An operating temperature of the first temperature electrode or the second temperature electrode is greater than or equal to 121 degrees Celsius.

5. The bioreactor exhaust pipe according to claim 2, characterized in that: The filter comprises a filter housing and a filter element. The filter housing is fixed on the filter pipeline, and the filter element is fixed in the filter housing.

6. A bioreactor, characterized in that: The bioreactor comprises a reactor tank body and a bioreactor exhaust pipe as described in any one of claims 1 to 5 connected to one end of the reactor tank body.

7. The bioreactor according to claim 6, characterized in that The reactor tank is provided with a pressure sensor for detecting the pressure in the exhaust pipe.

8. An exhaust duct control method, characterized in that: The bioreactor as claimed in any one of claims 6 to 7 comprises: When the pressure in the reactor tank is greater than or equal to a preset threshold, the filter pipeline of the current standby pipeline is opened, the filter pipeline of the working pipeline is closed, and the sterilization pipeline of the working pipeline is opened, wherein when the first pipeline is used as the working pipeline, the second pipeline is used as the standby pipeline, and when the second pipeline is used as the working pipeline, the first pipeline is used as the standby pipeline.

9. The exhaust duct control method according to claim 8, characterized in that: Also includes: After the sterilization pipeline of the working pipeline is opened for a preset time, the sterilization pipeline is closed.

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