A culture, automatic counting and traceability system for quantitative plate detection by enzyme substrate method

Abstract

The present invention discloses a culture, automatic counting and traceability system for enzyme substrate method quantitative plate detection, which includes a quantitative plate seat, a guide rail or a set of guide rails, a detection device, a first thruster, a second thruster, and a switcher. The present invention realizes positioning and guiding the quantitative plate seat to slide on the guide rail or the set of guide rails by providing a quantitative plate seat for placing the quantitative plate, a detection device for detecting the quantitative plate, a first thruster for pushing the quantitative plate seat at the corresponding layer of the guide rail or the set of guide rails to move back and forth between the detection position and the placement area, and a switcher for driving the first thruster to slide on any layer of the guide rail or the set of guide rails. The mechanical transmission structure combined with the shooting and image recognition of the detection device can automatically, quickly and accurately extract and identify the corresponding information of the quantitative plate placed in the quantitative plate seat. Further combined with the control system of the controller, the detection results of the quantitative plate placed inside the instrument after culture can be automatically calculated.

Description

Technical Field

[0001] The present invention relates to a culture, automatic counting and traceability system for enzyme substrate method quantitative plate detection. Background Art

[0002] For the quantitative detection of pathogenic bacteria such as total coliforms, fecal coliforms, Escherichia coli, Legionella pneumophila, Enterococcus faecalis, etc. based on the enzyme substrate method, the existing method is to encapsulate and culture in a quantitative plate, and then manually mark the wells with color changes, and observe the wells emitting fluorescence (for detecting Escherichia coli) under the irradiation of an ultraviolet lamp. The number of color changes and fluorescence emissions are respectively recorded and compared. Ultraviolet rays are harmful to the human eye, and the whole process needs to be manually operated, with high labor intensity and low detection efficiency. Since it takes at least 24 hours of incubation at a fixed temperature to count, strict requirements are imposed on manual operation and observation time. Even on holidays, if there is a detection task, it is still necessary to observe and count 24 hours later. Moreover, manual counting has a large error, is significantly affected by the background environmental light source, and subsequent look-up tables are required to obtain the results, which easily leads to deviation of the results. When performing fluorescence labeling, it is also necessary to manually mark under the ultraviolet lamp, exposing the skin to the ultraviolet lamp for a long time, causing harm to the detection personnel. Summary of the Invention

[0003] In order to solve the above problems, the present invention provides a culture, automatic counting and traceability system for enzyme substrate method quantitative plate detection.

[0004] The technical solution of the present invention to solve the existing problems is: A culture, automatic counting and traceability system for enzyme substrate method quantitative plate detection, as an improvement, includes a quantitative plate seat for placing and positioning a quantitative plate, and at least one such quantitative plate seat is provided; a guide rail or a set of guide rails for positioning and guiding the sliding of the quantitative plate seat, the guide rail or the set of guide rails are arranged in a layered manner, at least one layer of the guide rail or the set of guide rails is provided, and a seat body or a frame body for fixing the layered guide rail or the set of guide rails is provided. A clamping groove for positioning the quantitative plate seat is formed between the layered guide rail or the set of guide rails, and a detection position and a placement area are provided on each layer of the guide rail or the set of guide rails; a detection device is arranged at a position corresponding to the detection position of the guide rail or the set of guide rails, and the detection device is provided with a detection structure corresponding to each detection sample on the quantitative plate; a first thruster for pushing the quantitative plate seat at the corresponding layer of the guide rail or the set of guide rails to move back and forth between the detection position and the placement area; a second thruster for driving the relative movement of the detection device or / and the guide rail or the set of guide rails to facilitate detection; a switcher including a slide rail for installing and positioning the first thruster and a driving mechanism for driving the first thruster to slide at any layer of the guide rail or the set of guide rails to cooperate with the quantitative plate seat on any layer; an incubator, and the guide rail or the set of guide rails, the detection device, the first thruster, the second thruster, and the switcher are arranged in the incubator;

[0005] A controller for controlling and coordinating the operation of the system.

[0006] As a further improvement, the placement area is arranged at one or both ends of each layer of guide rails or guide rail groups, the detection position is arranged at the middle position of the guide rails or guide rail groups, the first thruster is provided with one or two groups corresponding to the number of placement area groups, and the switch is provided with one or two groups corresponding to the number of first thruster groups.

[0007] As a further improvement, the placement area is arranged at the upper and lower positions of each layer of guide rails or guide rail groups, the first thruster is provided with upper and lower two groups, and the switch is provided with two groups, which are respectively matched with the upper and lower groups of first thrusters.

[0008] As a further improvement, the quantitative disk seat in the upper end area is provided with a reset elastic body for returning to the upper end placement area.

[0009] As a further improvement, rolling bodies are arranged between the quantitative disk seat and the guide rails or guide rail groups to facilitate the sliding of the quantitative disk seat.

[0010] As a further improvement, the second thruster is arranged on the detection device, and the detection device is further provided with a camera, a QR code camera, a camera, a laser sensor or / and an RFID identifier; the detection device is provided with a second slide rail.

[0011] As a further improvement, the guide rails or guide rail groups include a pair of guide rods.

[0012] As a further improvement, the quantitative disk seat includes a pair of limit blocks that are limited between the guide rails or guide rail groups to form a clamping groove, and a bottom plate connecting the pair of limit blocks. Card slots for installing and positioning the quantitative disk are arranged at corresponding positions of the pair of limit blocks.

[0013] As a further improvement, the first thruster or / and the second thruster is a pneumatic rod, a hydraulic rod, a lead screw assembly with a driving motor or a linear motor.

[0014] As a further improvement, the driving mechanism includes a pneumatic rod, a hydraulic rod, a lead screw assembly with a driving motor or a linear motor.

[0015] As a further improvement, it further includes a controller for controlling and coordinating the operation of the system; the incubator is provided with a temperature regulator; the detection device further includes a memory and a communication port; a second lighting lamp and a second ultraviolet lamp are arranged in the incubator.

[0016] As a further improvement, the detection structure includes a circuit board, a light-shielding cover that can cover the detection sample, a first ultraviolet lamp, a first lighting lamp and a color sensor arranged on the circuit board and inside the light-shielding cover.

[0017] Compared with the prior art, the present invention is provided with a quantitative disk seat for placing a quantitative disk in an incubator, a sliding guide rail or a guide rail group for positioning and guiding the quantitative disk seat, a detection device for detecting the quantitative disk, a first thruster for pushing the quantitative disk seat at the corresponding layer of the guide rail or the guide rail group to move back and forth between the detection position and the placement area, and a switcher for driving the first thruster to slide on any layer of the guide rail or the guide rail group. The beneficial effects are that the mechanical transmission structures such as the quantitative disk seat, the sliding guide rail or the guide rail group, the first thruster and the switcher, under the control and drive of the controller, combined with the shooting and image recognition of the detection device, can automatically, quickly and accurately extract and identify the corresponding information of the quantitative disk placed in the quantitative disk seat, and further combined with the control system of the controller, can automatically calculate the detection results of the quantitative disk placed inside the instrument after cultivation. It can also realize traceability by adding a memory and a communication port.

[0018] The present invention integrates a system for quantitative disk cultivation, automatic counting and traceability. The system realizes real-time detection of cultivation, automatically observes and judges color changes and fluorescent well grids, and transmits detection results. There is no need for inspectors to observe cultivation results regularly on site, manually observe the number of positive wells and count. The system result output is fast and accurate, greatly reducing the labor intensity, improving the flexibility of detection time and the accuracy of detection results. Brief Description of the Drawings

[0019] Figure 1 is a schematic structural diagram of the present invention.

[0020] Figure 2 is a schematic diagram of the first thruster of the present invention pushing the quantitative disk seat.

[0021] Figure 3 is a schematic diagram of the first thruster of the present invention driving the quantitative disk seat to enter the detection position from the placement area.

[0022] Figure 4 is a schematic diagram of the cooperation of the quantitative disk seat, the guide rail or the guide rail group, the first thruster and the switcher of the present invention.

[0023] Figure 5 is Figure 4 an enlarged schematic diagram at position a of

[0024] Figure 6 is a schematic side view structure diagram of the cooperation between the quantitative disk and the quantitative disk seat.

[0025] Figure 7 is a schematic diagram of the cooperation between the detection structure and the detection sample.

[0026] Figure 8 is a schematic diagram of the upper and lower first thrusters pushing the quantitative disk seat. Detailed Embodiments

[0027] SeeFigure 1-8 , in this embodiment, a culture, automatic counting and traceability system for enzyme substrate quantitative plate detection includes a quantitative plate seat 2, a guide rail or a set of guide rails 3, a detection device 4, a first thruster 5, a second thruster 7, a switcher 6, an incubator 1, and a controller. The quantitative plate seat 2 is used to place and position the quantitative plate 21, and at least one quantitative plate seat 2 is provided.

[0028] The guide rail or the set of guide rails 3 is used to position and guide the sliding of the quantitative plate seat 2. The guide rail or the set of guide rails 3 is arranged in a layered manner, and at least one layer of the guide rail or the set of guide rails 3 is provided, and a seat body or a frame body 31 for fixing the layered guide rail or the set of guide rails 3 is provided. In this embodiment, upper and lower frame bodies 31 are adopted. The clamping grooves for positioning the quantitative plate seat 2 are formed between the layered guide rails or the set of guide rails 3, and detection positions and placement areas are provided on each layer of the guide rail or the set of guide rails 3; one or more quantitative plate seats 2 can be placed on each layer of the guide rail or the set of guide rails 3. See Figure 2 , 3 , in this embodiment, 2 quantitative plate seats can be placed in one placement area; Figure 8 , one quantitative plate seat 2 is placed in each of the placement areas at the upper end and the lower end.

[0029] The detection device 4 is arranged at a position corresponding to the detection position of the guide rail or the set of guide rails 3, and the detection device 4 is provided with a detection structure 41 corresponding one-to-one to the detection samples 11 on the quantitative plate 21. The first thruster 5 pushes the quantitative plate seat 2 at the corresponding layer of the guide rail or the set of guide rails 3 to move back and forth between the detection position and the placement area. One or more quantitative plate seats 2 can be placed in the placement area on each layer of the guide rail or the set of guide rails 3 according to actual conditions. One or more quantitative plate seats 2 on the placement area of each layer can be pushed by one first thruster 5 to pass through the detection position in sequence and cooperate with the detection structure 41 of the detection device 4 one by one for detection. The switcher 6 includes a slide rail 62 for installing and positioning the first thruster 5 and a driving mechanism 61 for driving the first thruster 5 to slide at any layer of the guide rail or the set of guide rails 3 to cooperate with the quantitative plate seat 2 on any layer.

[0030] The second thruster 7 drives the detection device 4 or / and the guide rail or the set of guide rails 3 to move relatively for the convenience of detection.

[0031] The incubator 1, the guide rail or the set of guide rails 3, the detection device 4, the first thruster 5, the second thruster 7, and the switcher 6 are arranged in the incubator 1. The incubator 1 can adopt the structure of an incubator with existing technology and can have the basic functions of an existing incubator. The controller controls and coordinates the operation of the system.

[0032] In order to utilize the usage space, the placement area is usually set at one or both ends of each layer of guide rails or guide rail groups 3, the detection position is set at the middle position of the guide rails or guide rail groups 3, the first thruster 5 is provided with one or two groups corresponding to the number of placement area groups, and the switch 6 is provided with one or two groups corresponding to the number of groups of the first thruster 5. Refer to Figure 2 、 3 The placement area is set at one end of each layer of guide rails or guide rail groups 3 and is located at the lower end. Accordingly, the first thruster 5 is set at one end and is located at the lower end, and a set of the switch 6 is also set and is located at the lower end. The other end of the guide rails or guide rail groups 3 is vacant or only a placement area and a detection position are set thereon. Refer to Figure 8 The placement areas are set at both ends of each layer of guide rails or guide rail groups 3 and are located at the upper and lower ends. Accordingly, the first thrusters 5 are set at both ends and are located at the upper and lower ends, and two sets of the switch 6 are also set and are located at the upper and lower ends.

[0033] Refer to Figure 1 、 4 、8. In this embodiment, the placement areas are set at the upper and lower positions of each layer of guide rails or guide rail groups 3, the first thrusters 5 are provided with two upper and lower groups, and the switch 6 is provided with two groups, which are respectively matched with the upper and lower groups of the first thrusters 5. The quantitative disk seat 2 in the upper end area is provided with a reset elastic body 24 for returning to the upper end placement area. When it is necessary to detect the upper end quantitative disk 21 on a certain layer of guide rails or guide rail groups 3, the driving mechanism 61 of the upper end group switch 6 drives the upper end first thruster 5 to the corresponding layer to be detected. After the upper end first thruster 5 enters this layer, the upper end first thruster 5 starts to push the quantitative disk seat 2 located in the placement area of this layer into the detection position. Then or simultaneously, the second thruster 7 drives the detection device 4 or / and the guide rails or guide rail groups 3 to move relatively, so that the detection structure 41 of the detection device 4 cooperates with the quantitative disk 21 on the quantitative disk seat 2 to detect the sample 11 on the quantitative disk 21. After the detection is completed, the upper end quantitative disk seat 2 can return to the placement area through the reset elastic body 24; the second thruster 7 drives the detection device 4 or / and the guide rails or guide rail groups 3 to move relatively away from the detection position.

[0034] Refer to Figure 2 、 3, if it is necessary to detect the metering disc 21 at the lower end of a certain layer, the drive mechanism 61 of the lower end group switch 6 drives the first pusher 5 at the lower end to the corresponding layer to be detected. After the first pusher 5 at the lower end enters this layer, the first pusher 5 at the lower end starts to push the metering disc seat 2 placed in the placement area of this layer into the detection position. Then or simultaneously, the second pusher 7 drives the detection device 4 or / and the guide rail or guide rail group 3 to move relatively, so that the detection structure 41 of the detection device 4 cooperates with the metering disc 21 on the metering disc seat 2 to detect the sample 11 on the metering disc 21. After the detection is completed, the metering disc seat 2 at the lower end usually returns to the placement area at the lower end by itself. The second pusher 7 drives the detection device 4 or / and the guide rail or guide rail group 3 to move relatively away from the detection position.

[0035] Under normal circumstances, the metering disc seats 2 at the upper and lower ends are located in the placement area as the default state. When it is necessary to detect the metering disc 21 on the guide rail or guide rail group 3 of a certain layer, the metering disc seat 2 of this layer will be pushed into the detection position by the corresponding first pusher 5.

[0036] In order to facilitate the cooperation between the metering disc seat 2 and the guide rail or guide rail group 3, rolling elements for facilitating the sliding of the metering disc seat 2 are provided between the metering disc seat 2 and the guide rail or guide rail group 3.

[0037] The second pusher 7 is arranged on the detection device 4. The detection device 4 can also be provided with a second slide rail 71, so that when the second pusher 7 drives the detection device 4, the detection device 4 can accurately slide at the detection position, so as to accurately detect the samples 11 on the metering discs 21 of all layers. The detection device 4 is also provided with a photographing camera 40, a two-dimensional code camera, a camera, a laser sensor or / and an RFID identifier. The second pusher 7 pushes the detection device 4 to move to an appropriate position range, and the appropriate position range is a position range that facilitates the corresponding detection structure on the detection device 4 to detect, or / and the detection camera to take pictures. The detection structure 41 includes a circuit board 42, a light-shielding cover 43 that can cover the detection sample 11, a first ultraviolet lamp 44, a first illuminating lamp 45 and a color sensor 46 arranged on the circuit board 42 and located inside the light-shielding cover 43.

[0038] Before the detection device 4 is started, it is at a certain distance from the metering disc. After starting, the second pusher 7 drives the detection device 4 to operate, and pushes the detection structure, color sensor, photographing camera 40, two-dimensional code camera, camera, laser sensor towards the metering disc, and takes multiple pictures and saves them for filing during the process of approaching the sample from far to near. After the detection device 4 reaches the suitable detection position and cooperates with the metering disc 21 of the layer to be detected, the relevant light sources on the detection structure 41 are started, and all the samples 11 on the metering discs 21, that is, the water samples, are recognized by the color sensor 46 to generate test results.

[0039] See Figure 6, in this embodiment, the quantitative disk seat 2 includes a pair of limit blocks 26 limited between the guide rails or the guide rail group 3 to form a clamping groove, and a bottom plate 22 connecting the pair of limit blocks 26. At the corresponding positions of the pair of limit blocks 26, there are clamping grooves 23 for installing and positioning the quantitative disk 21. The clamping grooves 23 of the limit blocks 26 can be used to install and position the quantitative disk 21. In this embodiment, the rolling elements are set as rollers 25, and the rollers 25 are arranged at the corresponding positions on the pair of limit blocks 26 and can roll and cooperate with the guide rails or the guide rail group 3. The clamping grooves 23 and the bottom plate 22 of the limit blocks 26 can well correct the plastic deformation of the quantitative disk.

[0040] The described guide rails or the guide rail group 3 includes a pair of guide rods 32.

[0041] In this embodiment, each layer of the track or the track group 3 includes a pair of guide rods 32 that cooperate with the quantitative disk seat 2. The quantitative disk seat 2 slides smoothly on the pair of guide rods 32 through the rollers 25. The seat body or the frame body 31 includes a pair of cooperating upper and lower frame bodies, and the multi-layer guide rods 32 are positioned and installed between the pair of upper and lower frame bodies.

[0042] The described first thruster 5 or / and the second thruster 7 is a pneumatic rod, a hydraulic rod, a lead screw assembly with a driving motor or a linear motor. The first thruster 5 or / and the second thruster 7 can also adopt other existing technical structures.

[0043] The described driving mechanism 61 includes a pneumatic rod, a hydraulic rod, a lead screw assembly with a driving motor or a linear motor. The driving mechanism 61 can also adopt other existing technical structures.

[0044] The incubator 1 is provided with a temperature regulator; the detection device 4 further includes a memory for storing detection data and a communication port for remote control and receiving detection data. A second lighting lamp and a second ultraviolet lamp are provided inside the incubator 1. The above-mentioned first and second lighting lamps can adopt LED lighting lamps.

[0045] The controller can be an independent computer or a touch screen 12 with an operating system integrated on the instrument. It can be connected to the detection device 4 in a wired or wireless manner such as wifi or Bluetooth through the communication port. The system of the present invention can also be equipped with a dedicated app client, and the detection report can be transmitted to the inspector's mobile phone client through the network. At the same time, the inspector can also observe the internal cultivation situation of the incubator system in real time through the mobile phone.

[0046] After the quantitative disk 21 is encapsulated, it is placed one by one on the corresponding quantitative disk seats 2 at the corresponding positions on the layered guide rails or the guide rail group 3 in the incubator 1. After the inspector sets the cultivation temperature through the operating device and starts the cultivation, after 24 - 28 hours of cultivation, the corresponding driving mechanism 61 and the corresponding first thruster 5 are automatically started to push the corresponding quantitative disk seat 2 to be inspected to the detection position.

[0047] The system provides ordinary light sources or fluorescence initiation conditions, obtains color images of the quantitative plate using a detection device, and can perform automatic interpretation and archiving, or this operation can also be carried out manually; uses color analysis software of the operation device to identify color change and fluorescence, marks the color change and fluorescence pore positions, and records the images again for easy analysis and research. Automatically generates the most probable number (MPN) of coliform bacteria under different detection items using calculation software; the system reads the identification on the quantitative plate, establishes detection file information, and automatically records and archives the entire detection process and results in the operation device for traceability of the detection results. The system comes with management functions such as permission management, user data management and analysis, and client management.

[0048] When the detection, cultivation, automatic counting and traceability system of the present invention is in use, the temperature of the incubator 1 can be set by the tester by adjusting the temperature regulator, or the temperature regulator can be automatically controlled by the program according to the specific bacterial culture type to determine the optimal culture temperature. The lighting lamp and ultraviolet lamp built in the incubator 1 or the detection structure 41 provide light sources or fluorescence initiation conditions, avoiding the influence of external light on the observation results. The color image of the quantitative plate 21 can be obtained using a camera, and automatic interpretation and archiving can be performed, or this operation can also be carried out manually; color change and fluorescence are identified using image analysis software, the color change and fluorescence pore cells are marked, and the images are recorded again for easy analysis and research; the most probable number (MPN) of coliform bacteria under different detection items is automatically generated using calculation software; the system reads the identification on the quantitative plate 21, establishes detection file information, and automatically records and archives the entire detection process and results through storage for traceability of the detection results. The system of the present invention comes with management functions such as permission management, user data management and analysis, and client management.

[0049] The system of the present invention can also automatically complete the collection of detection information and the archiving of detection results by identifying identifiers such as barcodes, QR codes or RFID tags on the quantitative plate 21, and upload them to the server and the client, thus realizing the full-cycle traceability of the test sample 11 and fundamentally avoiding problems such as fraud and non-traceability of the detection results.

Claims

1. A culture, automatic counting and traceability system for enzyme substrate quantitative plate detection, Characterized in that: It includes a quantitative plate base for placing and positioning a quantitative plate, and a plurality of the quantitative plate bases are provided; a guide rail or a set of guide rails for positioning and guiding the sliding of the quantitative plate base, the guide rail or the set of guide rails are arranged in a layered manner, the guide rail or the set of guide rails has at least one layer, and a seat body or a frame body for fixing the layered guide rail or the set of guide rails, and a clamping groove for positioning the quantitative plate base is formed between the layered guide rails or the set of guide rails, and a detection position and a placement area are provided on each layer of the guide rail or the set of guide rails; a detection device is arranged at a position corresponding to the detection position of the guide rail or the set of guide rails; a first thruster for pushing the quantitative plate base at the corresponding layer of the guide rail or the set of guide rails to move back and forth between the detection position and the placement area; a switcher including a slide rail for installing and positioning the first thruster and a driving mechanism for driving the first thruster to slide at any layer of the guide rail or the set of guide rails to cooperate with the quantitative plate base on any layer; a second thruster for driving the relative movement of the detection device and the guide rail or the set of guide rails for easy detection; an incubator, the guide rail or the set of guide rails, the detection device, the first thruster, the second thruster, and the switcher are arranged in the incubator; a controller for controlling and coordinating the operation of the system; the placement area is arranged at the upper and lower positions of each layer of the guide rail or the set of guide rails, the first thruster has upper and lower groups, and the switcher has two groups, which are respectively matched with the upper and lower groups of the first thruster.

2. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: The quantitative plate base in the upper end area is provided with a reset elastic body for returning to the upper end placement area.

3. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: A rolling body for facilitating the sliding of the quantitative plate base is provided between the quantitative plate base and the guide rail or the set of guide rails.

4. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: The detection device is provided with a detection structure corresponding to the detection samples on the quantitative plate one by one; the second thruster is arranged on the detection device, and the detection device is further provided with a camera, a two-dimensional code camera, a camera, a laser sensor or / and an RFID identifier; the detection device is provided with a second slide rail.

5. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: The guide rail or the set of guide rails includes a pair of guide rods.

6. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: The quantitative plate base includes a pair of limit blocks limited between the guide rail or the set of guide rails to form a clamping groove and a bottom plate connecting the pair of limit blocks, and a clamping groove for installing and positioning the quantitative plate is provided at the corresponding positions of the pair of limit blocks.

7. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, Characterized in that: The first thruster and / or the second thruster is a pneumatic rod, a hydraulic rod, a lead screw assembly provided with a driving motor or a linear motor; the driving mechanism includes a pneumatic rod, a hydraulic rod, a lead screw assembly provided with a driving motor or a linear motor.

8. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, characterized in that: the detection device further includes a memory and a communication port.

9. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 1, characterized in that: the incubator is provided with a temperature regulator; a second illuminating lamp and a second ultraviolet lamp are arranged in the incubator.

10. The culture, automatic counting and traceability system for enzyme substrate quantitative plate detection according to claim 4, characterized in that: the detection structure includes a circuit board, a light-shielding cover capable of covering the detection sample, a first ultraviolet lamp, a first illuminating lamp and a color sensor which are arranged on the circuit board and located in the light-shielding cover.

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