A multifunctional optical detection and analysis system

Abstract

The present invention provides a multifunctional optical detection and analysis system, comprising: an optical path detection unit, a sample processing unit, an optical path sorting unit, a signal conversion unit and a data processing unit. The multifunctional optical detection and analysis system provided by the present invention can realize the detection of multiple substances when the liquid sample is injected only once, which involves the detection of different optical analysis methods. At the same time, it can automatically control the removal and processing of liquid samples, distribute the original samples, pre-treated samples, and mixed samples to the detection optical path according to the set program, and automatically select the corresponding optical path configuration and optical analysis method to realize the quantitative detection of multiple different substances in the same liquid sample, making the multi-combination analysis more simple, reliable, efficient and safe.

Description

Technical Field

[0001] The present invention relates to the field of detection and analysis technology, and in particular to a multifunctional optical detection and analysis system. Background Art

[0002] Optical analysis refers to the method of chemical analysis using the optical properties of substances. Commonly used optical analyses include fluorescence photometry, transmitted light analysis, scattered light analysis and chemiluminescence analysis. These analysis methods are mature in technology, can analyze a wide variety of substances, and have a high level of automation. They are widely used in health inspection, environmental and food analysis, drug analysis, biochemistry and clinical testing and other fields.

[0003] However, most of the current analytical devices are basically equipped with one analytical method, such as a fully automatic biochemical analyzer based on transmitted light analysis and a fully automatic luminescent immunoassay analyzer based on chemiluminescence analysis. In actual scientific research and production work, it is often necessary to detect and analyze a variety of different substances in the same sample. Due to the limitations of the detection methods of the analytical device, even the detection and analysis of related substances in the same sample often requires the use of a variety of different analytical devices to achieve.

[0004] In addition, most detection and analysis require the use of solution samples that have been pre-treated by centrifugation, dissolution, etc. The sample pretreatment process requires a lot of manual intervention by the experimenter, which is the main limiting factor of laboratory automation. At the same time, sample exposure during the pretreatment process is also one of the main biosafety risks in the laboratory. Summary of the invention

[0005] In view of this, an object of the present invention is to provide a multifunctional optical detection and analysis system to solve the problem of single function of the detection and analysis device.

[0006] Based on the above objectives, the present invention provides a multifunctional optical detection and analysis system, characterized in that it includes:

[0007] An optical path detection unit, comprising at least two groups of detection optical paths, each group of the detection optical paths comprising a weak light detection optical path and a transmitted light detection optical path;

[0008] A sample processing unit, connected to the optical path detection unit through a pipeline, and used for removing and processing the sample;

[0009] An optical path sorting unit, used for providing a detection light beam for the optical path detection unit, comprising at least two groups of sorting optical paths, and the optical path sorting unit is connected to the optical path detection unit via an optical fiber;

[0010] A signal conversion unit, electrically connected to the optical path detection unit, configured to perform signal conversion on the optical signal output by the optical path detection unit and output a digital signal;

[0011] The data processing unit is electrically connected to the signal conversion unit and is used to analyze and process the digital signal output by the signal conversion unit and output analysis data.

[0012] Furthermore, the weak light detection optical path is used to detect scattered light or fluorescence of the sample, and the transmitted light detection optical path is used to detect transmitted light of the sample.

[0013] Furthermore, the detection pool in the detection optical path includes a detection area and a capillary, the capillary is arranged in the detection area, the capillary is a corrugated capillary, one end of the capillary is an inflow end, and the other end is an outflow end.

[0014] Furthermore, the output optical signal of the weak light detection optical path is filtered by a first filter and then sent to the photoelectric conversion unit. The first filter is assembled in a first circular filter wheel, and the first circular filter wheel is driven by a first stepper motor.

[0015] Furthermore, the sample processing unit includes an original sample sampling path, a pre-processed sample sampling path and at least one mixed sample sampling path, and the samples obtained through the original sample sampling path, the pre-processed sample sampling path and the mixed sample sampling path are all detected by the optical path detection unit.

[0016] Furthermore, the sample processing unit also includes: a cleaning passage and a compressed air passage, the pre-processed sample sampling passage and the mixed sample sampling passage are respectively connected to the cleaning passage, and the pre-processed sample sampling passage and the mixed sample sampling passage are respectively connected to the compressed air passage.

[0017] Furthermore, the sample processing unit includes an original sample sampling path and a multi-sample sampling path, and the samples obtained through the original sample sampling path and the multi-sample sampling path are detected by the optical path detection unit.

[0018] Furthermore, the multi-sample sampling passage includes a reaction disk provided with at least two reaction cups, and the samples in the reaction cups are detected by the optical path detection unit.

[0019] Furthermore, the detection optical path for detecting the sample in the reaction cup includes a detection module, and a detection slot cooperating with the reaction disk is provided inside the detection module.

[0020] Furthermore, the signal conversion unit includes a photoelectric conversion element and an analog-to-digital conversion element. The photoelectric conversion element converts the optical signal output by the optical path detection unit into an electrical signal, and the analog-to-digital conversion element converts the electrical signal into a digital signal.

[0021] From the above, it can be seen that the multifunctional optical detection and analysis system provided by the present invention can realize the detection of multiple substances when the liquid sample is injected only once, which involves the detection of different optical analysis methods. The system provided by the present invention can automatically control the removal and processing of liquid samples, distribute the original sample, pre-treated sample, and mixed sample to the detection light path according to the set program, automatically select the corresponding light path configuration and optical analysis method, and realize the quantitative detection of multiple different substances in the same liquid sample, making the multi-combination analysis more simple, reliable, efficient and safe. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 It is a structural schematic diagram of a multifunctional optical detection and analysis system according to an embodiment of the present invention;

[0024] Figure 2 is a structural schematic diagram of an optical path detection unit according to an embodiment of the present invention;

[0025] Figure 3 is a schematic structural diagram of a sample processing unit according to an embodiment of the present invention;

[0026] Figure 4 It is a schematic diagram of the structure of the detection cell according to an embodiment of the present invention;

[0027] Figure 5 A schematic diagram of the structure of a sample processing unit according to another embodiment of the present invention;

[0028] Figure 6 It is a structural schematic diagram of a reaction disk according to an embodiment of the present invention;

[0029] Figure 7 A schematic structural diagram of an optical path detection unit according to another embodiment of the present invention;

[0030] Figure 8 Schematic diagram of the structure of an optical path sorting unit according to an embodiment of the present invention.

[0031] Reference numerals:

[0032] 1. Optical path detection unit; 11. First collimator; 12. First detection pool; 121. Inflow end; 122. Outflow end; 123. Capillary; 124. Detection area; 13. First lens; 141. First filter; 142. First circular filter wheel; 15. First stepper motor; 16. First optical fiber interface; 17. First optical fiber; 18. Detection module; 181. Detection slot; 182. Second collimator; 183. Second lens; 184. Second optical fiber interface; 185. Third optical fiber interface; 186. Fourth optical fiber interface; 187. Third lens; 188. Third filter; 189. Third circular filter light wheel; 1891, third stepper motor; 2, sample processing unit; 211, first original sample tube; 212, first sampling needle; 213, first rotary sampling valve; 214, first selection valve; 215, first plunger pump; 216, first quantitative sample loop; 221, first reagent bottle; 222, first reagent pump; 223, first mixing pool; 224, second rotary sampling valve; 2241, second quantitative sample loop; 225, third rotary sampling valve; 2251, third quantitative sample loop; 226, second selection valve; 227, second detection pool; 228, second plunger pump; 231, second reagent bottle; 232, 2nd reagent pump; 233, 2nd mixing tank; 241, 3rd reagent bottle; 242, 3rd reagent pump; 243, 3rd mixing tank; 251, air compression pump; 252, 1st air solenoid valve; 253, 2nd air solenoid valve; 254, 3rd air solenoid valve; 261, cleaning liquid reagent bottle; 262, 1st cleaning solenoid valve; 263, cleaning pump; 264, 2nd cleaning solenoid valve; 265, 3rd cleaning solenoid valve; 266, 4th cleaning solenoid valve; 267, waste pump; 268, 5th cleaning solenoid valve; 271, reaction disk; 2711, reaction cup; 2712, sample adding system; 2713, test reagent distribution system; 272, second sampling needle; 273, fourth rotary sampling valve; 2731, fourth quantitative sample ring; 274, third selection valve; 275, third detection pool; 276, third plunger pump; 277, fourth reagent bottle; 278, fourth reagent pump; 281, second original sample tube; 282, third sampling needle; 3, optical path sorting unit; 31, light source; 32, fifth optical fiber interface; 33, third collimator; 34, second filter; 341, second circular filter wheel; 35, sixth optical fiber interface; 36, second optical fiber; 37, second stepping motor; 4, signal conversion unit; 5, data processing unit. DETAILED DESCRIPTION

[0033] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0034] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the embodiments of the present invention should be understood by people with ordinary skills in the field to which the present disclosure belongs. The "first", "second" and similar words used in the present disclosure do not represent any order, quantity or importance, but are only used to distinguish different components. "Including" or "comprising" and similar words mean that the elements or objects appearing in front of the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0035] A multifunctional optical detection and analysis system provided by the present invention is described in detail below with reference to the accompanying drawings.

[0036] refer to Figure 1 The present invention provides a multifunctional optical detection and analysis system, comprising:

[0037] The optical path detection unit 1 includes at least two groups of detection optical paths, each group of the detection optical paths includes a weak light detection optical path and a transmitted light detection optical path, and can perform multiple optical analyses on the sample, and the two groups of detection optical paths are independent of each other. The optical path structures of the two groups of detection optical paths can be set to be the same or different, and can be adjusted according to the specific detection situation. The number of detection optical paths is not limited to two groups, and multiple groups can be set, so that the sample can be optically detected and analyzed simultaneously or in a time-sharing manner.

[0038] The sample processing unit 2 is connected to the optical path detection unit 1 through a pipeline, and is used to transfer and process the sample. It can automatically sample and pre-process the original sample, mix it with other different reagents, and automatically prepare multiple mixed samples, thereby reducing manual intervention, reducing manual workload, and reducing sample exposure time.

[0039] The optical path sorting unit 3 is used to provide a detection light beam for the optical path detection unit 1, and includes at least two groups of sorting light paths. The optical path sorting unit 3 is connected to the optical path detection unit 1 through an optical fiber. The light source of the optical path sorting unit 3 is a wide-spectrum or multi-spectral light source. Through the conversion of optical elements, a spectrum light source or a monochromatic light source that meets the detection requirements can be output.

[0040] The signal conversion unit 4 is electrically connected to the optical path detection unit 1, and is used to convert the optical signal output by the optical path detection unit 1 to output a digital signal. The optical signal is converted by the signal conversion element in the signal conversion unit 4, and then converted into a digital signal for analysis and processing by the data processing unit 5.

[0041] The data processing unit 5 is electrically connected to the signal conversion unit 4, and is used to analyze and process the digital signal output by the signal conversion unit 4. The computer and dedicated software obtain the concentration of the object to be measured in the test sample based on the light intensity data-concentration algorithm of the corresponding object to be measured, complete the optical analysis, output the analysis data, and complete the detection of the specified substance in the original sample placed in the detection and analysis system.

[0042] In some embodiments, the weak light detection optical path is used to detect scattered light or fluorescence of the sample, and the transmitted light detection optical path is used to detect transmitted light of the sample. Figure 1 The monochromatic light output from the optical path sorting unit 3 is collimated and then irradiated onto the surface of the sample to be detected. The monochromatic light is used as the excitation light for fluorescence photometric analysis of the sample to be detected, or as the incident light source for transmitted light and scattered light analysis. The fluorescence emitted by the sample to be detected after excitation and the scattered light generated by reflecting the incident light are both detected through the weak light detection optical path, and the transmitted light formed by the sample to be detected after being irradiated by the incident light is detected through the transmitted light detection optical path.

[0043] In some embodiments, the detection pool in the detection light path includes a detection area 124 and a capillary 123 . The capillary 123 is disposed in the detection area 124 . The capillary 123 is a corrugated capillary. One end of the capillary 123 is an inflow end 121 , and the other end is an outflow end 122 .

[0044] In this embodiment, the first detection pool 12 and the second detection pool 227 are flow detection pools, such as Figure 4 As shown. The flow detection pool is a flat rectangular quartz glass or transparent polymer material product, including a detection area 124 and a capillary 123 therein. The capillary 123 is a corrugated capillary. The two ends of the capillary 123 are respectively a liquid inlet end 121 and a liquid outlet end 122. The inlet end 121 and the outlet end 122 are connected in series in the fluid processing pipeline for the inflow and outflow of samples and other fluids. The detection area 124 refers to the area within the minimum envelope circle of the capillary 123. The inner diameter of the capillary 123 is uniform, and the applicable size of the inner diameter is 0.1mm-1.6mm. The total length of the capillary 123 and its minimum envelope circle, that is, the area ratio of the detection area 124 is ≥0.3mm / mm 2Compared with straight capillaries, corrugated capillaries have a larger liquid volume and can store more test samples. During the test process, the area of ​​light absorption or reflection by the sample increases accordingly, which can effectively improve the optical test accuracy.

[0045] The flow detection cell in this embodiment is suitable for detecting various low-viscosity liquid samples. It is not limited to the application method in this embodiment, and can also be used in other fluorescence analysis, luminescence analysis, scattered light analysis, transmitted light detection, image analysis and other devices.

[0046] In some embodiments, reference Figure 2 , the output optical signal of the weak light detection optical path is filtered by the first filter 141 and then sent to the signal conversion unit 4, the first filter 141 is assembled in the first circular filter wheel 142, and the first circular filter wheel 142 is driven by the first stepper motor 15. Specifically, the optical path detection unit 1 includes two groups of identical detection optical paths, and the weak light detection optical path of each group of detection optical paths sequentially includes the first collimator 11, the first detection cell 12, the first lens 13 and the first filter 141. The incident light emitted by the optical path sorting unit 3 is collimated by the first collimator 11 and then irradiated to the surface of the sample to be detected in the first detection cell 12. The light emitted from the first detection cell 12 is converged and collimated by the first lens 13, and then filtered by the first filter 141 and irradiated to the signal conversion element of the signal conversion unit 4. The first filter 141 is mounted on the first circular filter wheel 142. Multiple groups of filters with different wavelengths can be mounted in the first circular filter wheel 142. By rotating the first circular filter wheel 142, first filters 141 with different wavelengths can be selected. The first circular filter wheel 142 is driven to rotate by the first stepper motor 15. The first stepper motor 15 is controlled by the system to rotate according to the optical path parameter setting corresponding to the sample to be detected in the detection pool, and the first circular filter wheel 142 is driven to rotate, and the first filter 141 installed in the first circular filter wheel 142 is selected to the detection optical path to complete the optical detection of the weak light detection optical path. The transmitted light detection optical path includes the first collimator 11, the first detection pool 12 and the first optical fiber interface 16 in sequence. After the incident light passes through the first collimator 11, it irradiates the first detection pool 12, and forms transmitted light on the other side of the first detection pool 12. The transmitted light passes through the first optical fiber interface 16 and then is transmitted to the signal conversion unit 4 through the first optical fiber 17. The first optical fiber interface 16 in this embodiment is a microscope optical fiber interface.

[0047] The two sets of detection optical paths in the optical path detection unit 1 can realize the detection of characteristic optical data of two different samples. In this embodiment, the characteristic optical data detection includes fluorescence detection, transmitted light detection and scattered light detection. According to actual detection requirements, the detection optical path can also be adjusted to realize other characteristic optical data detection, which is not limited to the three characteristic optical data proposed in this embodiment.

[0048] In some embodiments, the sample processing unit 2 includes an original sample sampling path, a pre-processed sample sampling path and at least one mixed sample sampling path, and the samples obtained through the original sample sampling path, the pre-processed sample sampling path and the mixed sample sampling path are detected by the optical path detection unit 1. The sample processing unit 2 can detect the original sample, and can also detect the pre-processed sample and the mixed sample, so that the detection of multiple substances can be achieved. One mixed sample sampling path corresponds to one mixed sample, and a mixed sample sampling path can be added according to actual conditions, and is not limited to one mixed sample sampling path in the present embodiment.

[0049] In some embodiments, the sample processing unit 2 further includes: a cleaning passage and a compressed air passage, the pre-processed sample sampling passage and the mixed sample sampling passage are respectively connected to the cleaning passage, and the pre-processed sample sampling passage and the mixed sample sampling passage are respectively connected to the compressed air passage. The cleaning passage can clean the mixing pool of the pre-processed sample and the mixed sample, and the compressed air passage fully mixes the samples in the mixing pool by delivering compressed air to improve the sample detection accuracy.

[0050] refer to Figure 3 The sample processing unit 2 includes an original sample sampling passage, a pre-processed sample sampling passage, two mixed sample sampling passages, a cleaning passage and a compressed air passage. The original sample sampling passage includes a first original sample tube 211, a first sampling needle 212, a first rotary sampling valve 213, a first selection valve 214, a first detection pool 12 and a first plunger pump 215 in sequence. The first rotary sampling valve 213 rotates to the sampling position, and the sampling needle 212 is driven by a motor to enter the original sample tube 211. The original sample sampling passage absorbs the original sample from the original sample tube 211. A part of the absorbed original sample enters the first detection pool 12 for detection, and the other part of the original sample is filled into the first quantitative sample ring 216 in the first rotary sampling valve 213. At this point, the sampling is completed, and the motor drives the sampling needle 212 to move out of the sample tube. When the original sample in the first detection pool 12 has been tested, the first plunger pump 215 pushes pure water into the first detection pool 12 to clean the first detection pool 12. The first plunger pump 215 pushes the waste water after cleaning to be discharged from the waste discharge port of the first selection valve 214 to prepare for the next suction test of the sample.

[0051] The pretreatment sample sampling path includes the first reagent bottle 221, the first reagent pump 222, the first rotary sampling valve 213, the first mixing pool 223, the second rotary sampling valve 224, the third rotary sampling valve 225, the second selection valve 226, the second detection pool 227 and the second plunger pump 228 in sequence. Specifically, the first rotary sampling valve 213 rotates to the distribution position, the first reagent pump 222 discharges the pretreatment liquid sucked from the first reagent bottle 221, and pushes the original sample filled in the first quantitative sample loop 216 to be sequentially injected into the detection pool 227. In the first mixing pool 223, the pretreatment liquid is mixed with the original sample to form the pretreatment sample. The second rotary sampling valve 224 and the third rotary sampling valve 225 rotate to the sampling position. The pretreatment sample sampling passage draws the pretreatment sample from the first mixing pool 223. A part of the drawn pretreatment sample enters the second detection pool 227 for detection, and the other part fills the second quantitative sample ring 2241 and the third quantitative sample ring 2251 in the second rotary sampling valve 224 and the third rotary sampling valve 225, completing the sampling of the pretreatment sample. When the pretreatment sample in the second detection pool 227 is detected, the second plunger pump 228 drives the injection of pure water into the second detection pool 227 for cleaning. The cleaned waste water is driven by the second plunger pump 228 to be discharged from the second selection valve 226, realizing the flushing of the second detection pool 227, so as to prepare for the next suction detection of the sample.

[0052] The sample sampling passage of the first mixed sample includes the second reagent bottle 231, the second reagent pump 232, the second rotary sampling valve 224, the second mixing pool 233, the second selection valve 226, the second detection pool 227 and the second plunger pump 228 in sequence, and the sample sampling passage of the second mixed sample includes the third reagent bottle 241, the third reagent pump 242, the third rotary sampling valve 225, the third mixing pool 243, the second selection valve 226, the second detection pool 227 and the second plunger pump 228 in sequence. The second rotary sampling valve 224 and the third rotary sampling valve 225 are rotated to the distribution position, the second reagent pump 232 discharges the reagent sucked from the second reagent bottle 231, and pushes the pre-treated sample filled in the second quantitative sample loop 2241 to be sequentially injected into the second mixing pool 233, the third reagent pump 242 discharges the reagent sucked from the third reagent bottle 241, and pushes the pre-treated sample filled in the third quantitative sample loop 2251 to be sequentially injected into the third mixing pool 243, and the reagent and the pre-treated sample are mixed to form a mixed sample. Through the pipeline connected to the second selection valve 226, the mixed samples in the second mixing tank 233 and the third mixing tank 243 are sucked into the second detection tank 227 by the second plunger pump 228 through the second selection valve 226 for detection.

[0053] In this embodiment, the pretreated samples in the first mixing pool 223 and the mixed samples in the second mixing pool 233 and the third mixing pool 243 can also be connected to the first selection valve 214 through pipelines, and sucked into the first detection pool 12 by the first plunger pump 215 for detection.

[0054] The air compression passage includes an air compression pump 251 and a first air solenoid valve 252, a second air solenoid valve 253, and a third air solenoid valve 254 in the passage. The first air solenoid valve 252 is pulsed for multiple times, and a small amount of compressed air is pulsed into the first mixing pool 223 through the air compression pump 251 to fully mix the pre-treated sample. The second air solenoid valve 253 and the third air solenoid valve 254 are pulsed for multiple times, and a small amount of compressed air is pulsed into the second mixing pool 233 and the third mixing pool 243 through the air compression pump 251 to fully mix the mixed sample.

[0055] The cleaning passage includes a cleaning liquid reagent bottle 261, a first cleaning solenoid valve 262, a cleaning pump 263, a fifth cleaning solenoid valve 268, a second cleaning solenoid valve 264, a third cleaning solenoid valve 265, a fourth cleaning solenoid valve 266 and a waste pump 267. The corresponding second cleaning solenoid valve 264, the third cleaning solenoid valve 265 and the fourth cleaning solenoid valve 266 connected to the waste pump 267 are respectively connected, and the waste pump 267 is started to empty the residual liquid in the relevant first mixing pool 223, the second mixing pool 233 and the third mixing pool 243. Then, the fifth solenoid valve 268 and the cleaning solenoid valves connected to each mixing pool are connected, and the cleaning liquid in the cleaning liquid reagent bottle 261 is injected into each mixing pool through the cleaning pump 263, and then each mixing pool is emptied to complete a cleaning. The first solenoid valve 262 is connected, and pure water is injected into each mixing pool through the cleaning pump 263, and then each mixing pool is emptied successively to complete a rinse. Each mixing pool after cleaning and rinsing can be used for mixing the next sample.

[0056] The inner cavity of the mixing pool in this embodiment is cylindrical or elliptical, and each mixing pool includes at least four pipe connection ports, namely, a sample transfer port, a sample removal port, an air inlet port, and an emptying / cleaning port. Except for the connection ports, the mixing pool in this embodiment is a fully enclosed structure.

[0057] In some embodiments, the sample processing unit 2 includes an original sample sampling path and a multi-sample sampling path, and the samples obtained through the original sample sampling path and the multi-sample sampling path are detected by the optical path detection unit 1. Figure 5, the original sample sampling path remains unchanged, and the pre-treatment sample sampling path and the mixed sample sampling path are replaced by the multi-sample sampling path, which includes the fourth reagent bottle 277, the fourth reagent pump 278, the fourth rotary sampling valve 273, the second sampling needle 272, and the reaction disk 271 in sequence. The original sample sampling path in this embodiment includes the second original sample tube 281, the third sampling needle 282, the fourth rotary sampling valve 273, the third selection valve 274, the third detection pool 275 and the third plunger pump 276 in sequence. The fourth rotary sampling valve 273 rotates to the sampling position, and the motor drives the third sampling needle 282 to enter the second original sample tube 281, and the original sample is sucked from the second original sample tube 281. A part of the sucked original sample enters the third detection pool 275 for detection, and the other part is filled into the fourth quantitative sample ring 2731 in the fourth rotary sampling valve 273. At this point, the sampling is completed, and the motor drives the third sampling needle 282 to move out of the second original sample tube 281. After the original sample in the third detection pool 275 is tested, pure water is injected through the third plunger pump 276 for cleaning. The waste water after cleaning is pushed by the third plunger pump 276 to be discharged from the waste discharge port of the third selection valve 274, thereby flushing the third detection pool 275 in preparation for the next sample to be tested.

[0058] The fourth rotary sampling valve 273 rotates to the distribution position, and the fourth reagent pump 278 discharges the pretreatment liquid sucked from the fourth reagent bottle 277, and pushes the original sample filled in the fourth quantitative sample ring 2731 to be added to the reaction disk 271 to mix as a pretreatment sample, and then the pretreatment sample and the reagent are mixed to prepare a mixed sample. The reaction disk 271 is driven to rotate by an external motor, and the pretreatment sample and the mixed sample that have completed the reaction are rotated to the optical path detection module 18 composed of optical elements to complete the characteristic light detection. The optical path detection module 18 is included in the optical path detection unit 1.

[0059] In some embodiments, reference Figure 6, the multi-sample sampling passage includes a reaction disk 271 provided with at least two reaction cups 2711, and the sample in the reaction cup 2711 is detected by the optical path detection unit 1. Specifically, the reaction disk 271 includes a plurality of reaction cups 2711 equidistantly arranged at the edge of the reaction disk 271 in the circumferential direction, and each reaction cup 2711 also serves as a detection cup. The reaction disk 271 cooperates with the sample adding system 2712 and the reagent dispensing system 2713 to perform multiple sampling and distribution of the pre-treated sample, and multiple mixed samples are formed by sequentially adding different reagents to the reaction cups 2711 for mixed reaction. The sample and pre-treatment liquid injected from the fourth rotary sampling valve 273 are stored in one of the reaction cups 2711 and mixed to form a pre-treated sample. The sample adding system 2712 samples from the reaction cup 2711 multiple times and distributes them to other reaction cups 2711, and the reagent dispensing system 2713 injects different reaction reagents into them respectively, thereby forming multiple mixed samples.

[0060] In some embodiments, the detection optical path for detecting the sample in the reaction cup 2711 includes a detection module 18, and a detection slot 181 is provided inside the detection module 18 to cooperate with the reaction disk 271. Figure 7The optical path detection unit 1 includes two groups of detection optical paths, one of which is used to detect the third detection pool 275. The detection method is the same as described above and will not be repeated here. The other group of detection optical paths is used to detect the sample in the reaction cup 2711 of the reaction disk 271. The detection module 18 is placed under the reaction disk 271 so that the detection cup 2711 is in the detection slot 181 of the detection module 18. The width of the detection slot 181 is greater than the thickness of the detection cup 2711 in the reaction disk 271 to ensure that each detection cup 2711 in the reaction disk 271 can be smoothly moved and detected in the detection slot 181. The detection module 18 includes a second collimator 182, a second lens 183, a second optical fiber interface 184, and a third optical fiber interface 185, wherein the second collimator 182, the second lens 183, and the second optical fiber interface 184 are included in the weak light detection optical path, and the third optical fiber interface 185 is included in the transmitted light detection optical path. The incident light from the optical path sorting unit 3 is collimated by the second collimator 182 and then irradiated to the sample surface in one of the detection cups 2711 of the reaction disk 271. The scattered light generated by the sample reflection or the fluorescence generated by the sample excitation is converged and collimated by the second lens 183 and then incident to the second optical fiber interface 184. Then, it is converged and collimated by the fourth optical fiber interface 186 and the third lens 187 and then incident to the third filter 188. The light emitted by the third filter 188 is irradiated to the signal conversion unit 4 for signal conversion. The third filter 188 is assembled in the third circular filter wheel 189, and the third circular filter wheel 189 is driven by the third stepping motor 1891. The combined use of the detection cell and the detection cup in this embodiment is suitable for the detection requirements of combining multi-stage processing with different optical analysis methods. Compared with the use of a single detection cell, it can conveniently prepare more reagent mixed samples and realize more rapid quantitative detection of more substances.

[0061] In some embodiments, the sorting optical path includes a second filter 34 for outputting a monochromatic light source, the second filter 34 is assembled in a second circular filter wheel 341, and the second circular filter wheel 341 is driven by a second stepping motor 37. Figure 8The optical path sorting unit 3 includes two groups of sorting optical paths. In this embodiment, the two groups of sorting optical paths have the same structure. Each group of sorting optical paths includes a light source 31, a fifth optical fiber interface 32, a third collimator 33, a second filter 34 and a sixth optical fiber interface 35. The light source 31 is a broadband or multi-spectral light source. The broadband light emitted by the light source 31 is transmitted to the third collimator 33 through the fifth optical fiber interface 32 and the second optical fiber 36, and is divided into two broadband light outputs, which are collimated into two parallel light beams by the third collimator 33. Then, two monochromatic lights are filtered by the second filter 34, and the two monochromatic lights are respectively irradiated to the light receiving surfaces of the two symmetrically arranged sixth optical fiber interfaces 35, and converged in the sixth optical fiber interface 35 to output monochromatic lights that can be transmitted by optical fibers. The wavelength of the monochromatic light is determined by the second filter 34, which is mounted in the second circular filter wheel 341. Multiple groups of narrow-band filters can be mounted in the second circular filter wheel 341, and the second circular filter wheel 341 is driven to rotate by the second stepping motor 37. The optical path sorting unit 3 can output two paths of monochromatic light with different wavelengths at the same time, and the two paths of different monochromatic light are a group. According to the system instructions, the optical path sorting unit 3 can output multiple groups of designated monochromatic light groups with different wavelengths in time.

[0062] In some embodiments, the signal conversion unit 4 includes a photoelectric conversion element and an analog-to-digital conversion element. The photoelectric conversion element converts the optical signal output by the optical path detection unit 1 into an electrical signal, and the analog-to-digital conversion element converts the electrical signal into a digital signal. In this embodiment, the photoelectric conversion element includes a photomultiplier tube and a photodiode. The photomultiplier tube is a photoelectric detector for weak light such as fluorescence and scattered light, and the photodiode is a photoelectric detector for transmitted light. The optical signal is converted into an electric current by the photodetector, and then processed by signal amplification, and converted into light intensity data by the analog-to-digital conversion element A / D, thereby realizing the detection of the characteristic optical data of the sample to be detected. Both the photomultiplier tube and the photodiode can be replaced by image detection devices such as CCD and CMOS, so that image analysis and spectral analysis of the sample to be detected can be realized.

[0063] The workflow of the multifunctional optical detection and analysis system proposed by the present invention is described below:

[0064] Workflow 1:

[0065] The original sample is placed in the multifunctional optical detection and analysis system, and is distributed and processed into four kinds of samples by the sample processing unit 2, namely, the original sample, the pre-processed sample and two mixed samples. The structure of the sample processing unit 2 is as follows: Figure 3 As shown, the optical path detection unit 1 is as shown in Figure 2 As shown. Figure 3As shown, the original sample and the pre-treated sample are respectively filled into the first detection pool 12 and the second detection pool 227 for detection. After the detection is completed, the first detection pool 12 and the second detection pool 227 are emptied and cleaned respectively, and then the two mixed samples are detected again, and the detection method is the same as above.

[0066] The characteristic light detection of the four samples all passes through the optical path detection unit 1. According to the characteristic wavelength of the excitation light of the sample to be detected, the optical path sorting unit 3 outputs the corresponding characteristic monochromatic light group according to the system instruction and transmits it to the optical path detection unit 1. The sample to be detected is excited by the characteristic monochromatic light to generate characteristic emission light. After the stray light is filtered out by the corresponding filter selected by the system instruction in the optical path detection unit 1, it is detected through the weak light detection optical path. This process is a fluorescence detection process.

[0067] According to the characteristic wavelength of the absorption light of the sample to be detected, the optical path sorting unit 3 outputs the corresponding characteristic monochromatic light group according to the system instruction and transmits it to the optical path detection unit 1. The characteristic monochromatic light is partially absorbed by the sample to be detected. The unabsorbed transmitted light is transmitted to the signal conversion unit 4 through the first optical fiber interface 16 in the optical path detection unit 1 through the first optical fiber 17 for detection. The intensity of the transmitted light is proportional or inversely proportional to the concentration of the sample to be detected. This process is the transmitted light detection process, which is detected through the transmitted light detection optical path.

[0068] According to the characteristic wavelength of the absorption light of the sample to be detected, the optical path sorting unit 3 outputs the corresponding characteristic monochromatic light group according to the system instruction, and transmits it to the optical path detection unit 1. The characteristic monochromatic light is partially scattered by the sample to be detected. According to the system instruction, the corresponding scattered light filter group is selected in the optical path detection unit 1, and the scattered light enters the signal conversion unit 4 and is detected. This process is the scattered light detection process, which is detected through the weak light detection optical path.

[0069] The above three optical signals are converted into digital light intensity data that can be recognized, stored and called by computer software by the signal conversion unit 4. The light intensity data is further analyzed and calculated by the data processing unit 5 to obtain the concentration of the object to be tested in the sample to be tested, complete the optical analysis, output the analysis data, and complete the detection of the specified substance in the original sample.

[0070] In summary, in this process, after the original sample is placed, it is divided into 4 types of samples. It is successively filled into 2 independent detection pools for detection, and then passes through 2 sets of independent optical paths to detect at least 4 characteristic light data. According to the corresponding optical analysis method, quantitative detection of at least 4 different substances in the original sample is achieved.

[0071] Workflow 2:

[0072] The difference between this process and workflow 1 is: optical path detection unit 1 and sample processing unit 2. Figure 7 , sample processing unit reference Figure 5and Figure 6 . The original sample is placed in the sample processing unit 2. The sampling and distribution of the original sample and the pretreatment liquid are the same as those in the workflow 1. The reaction disc 271 is used for processing when forming the pretreatment sample and preparing the mixed sample. Through the cooperation of the sample addition system 2712 and the reagent distribution system 2713, multiple mixed samples are formed in the reaction cup 2711. After the mixed sample is prepared, the reaction disc 271 is rotated to the detection module 18 for optical detection. The reaction disc 271 is placed in the detection slot 181. Different reaction cups 2711 are aligned to the detection slot 181 by rotation to complete the detection of different mixed samples. The detection of the pretreatment sample is also completed in the reaction cup 2711. The original sample is detected in the third detection pool 275, and the detection principle is the same as that in the workflow 1.

[0073] For different samples to be detected, three different characteristic lights, namely fluorescence, transmitted light and scattered light, can be detected respectively by the optical path detection unit 1, and the corresponding characteristic optical signals can be obtained by the signal conversion unit 4. Finally, the optical analysis is completed by the data processing unit 5 to output the optical data.

[0074] This process uses an analytical device that combines a detection cell with a detection cup, which can perform multi-level distribution and processing of samples. It is suitable for specific samples that require multi-level processing combined with different optical analysis methods and combined detection of multiple substances.

[0075] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present disclosure (including the claims) is limited to these examples. Within the scope of the present invention, the above embodiments or technical features in different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0076] The embodiments of the present invention are intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A multifunctional optical detection and analysis system, characterized in that: include: An optical path detection unit, comprising at least two groups of detection optical paths, each group of the detection optical paths comprising a weak light detection optical path and a transmitted light detection optical path; A sample processing unit is connected to the optical path detection unit through a pipeline and is used to remove and process the sample; the sample processing unit includes an original sample sampling passage, a pre-processed sample sampling passage, a first mixed sample sampling passage, and a second mixed sample sampling passage; The original sample sampling path includes, in sequence, a first original sample tube, a first sampling needle, a first rotary sampling valve, a first selection valve, a first detection cell and a first plunger pump; The pre-treatment sample sampling passage sequentially includes a first reagent bottle, a first reagent pump, a first rotary sampling valve, a first mixing tank, a second rotary sampling valve, a third rotary sampling valve, a second selection valve, a second detection tank, and a second plunger pump; The first mixed sample sampling passage sequentially includes a second reagent bottle, a second reagent pump, a second rotary sampling valve, a second mixing tank, a second selection valve, a second detection tank and a second plunger pump; The second mixed sample sampling passage sequentially includes a third reagent bottle, a third reagent pump, a third rotary sampling valve, a third mixing tank, a second selection valve, a second detection tank and a second plunger pump; An optical path sorting unit, used for providing a detection light beam for the optical path detection unit, comprising at least two groups of sorting light paths, and the optical path sorting unit is connected to the optical path detection unit via an optical fiber; A signal conversion unit, electrically connected to the optical path detection unit, and configured to perform signal conversion on the optical signal output by the optical path detection unit and output a digital signal; The data processing unit is electrically connected to the signal conversion unit and is used to analyze and process the digital signal output by the signal conversion unit and output analysis data.

2. The analysis system according to claim 1, characterized in that The weak light detection optical path is used to detect scattered light or fluorescence of a sample, and the transmitted light detection optical path is used to detect transmitted light of a sample.

3. The analysis system according to claim 1, characterized in that The detection pool in the detection optical path comprises a detection area and a capillary. The capillary is arranged in the detection area. The capillary is a corrugated capillary. One end of the capillary is an inflow end, and the other end is an outflow end.

4. The analysis system according to claim 1, characterized in that The output optical signal of the weak light detection optical path is filtered by a first filter and then sent to the signal conversion unit. The first filter is assembled in a first circular filter wheel, and the first circular filter wheel is driven by a first stepper motor.

5. The analysis system according to claim 1, characterized in that The sample processing unit also includes: a cleaning passage and a compressed air passage, the pretreatment sample sampling passage, the first mixed sample sampling passage and the second mixed sample sampling passage are respectively connected to the cleaning passage, and the pretreatment sample sampling passage, the first mixed sample sampling passage and the second mixed sample sampling passage are respectively connected to the compressed air passage.

6. The analysis system according to claim 1, characterized in that The signal conversion unit includes a photoelectric conversion element and an analog-to-digital conversion element. The photoelectric conversion element converts the optical signal output by the optical path detection unit into an electrical signal, and the analog-to-digital conversion element converts the electrical signal into a digital signal.

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