A cell culture box for culturing adherent cells

Abstract

The present invention relates to a cell culture box for adherent cell culture, comprising: a box body (1) with a heating function, a box cover (2), a temperature and humidity regulating device (3) arranged on one side of the box body (1) and located outside the box body (1), and a culture dish support platform (4) arranged in the box body (1); mesh holes (1a) are regularly arranged on one side of the box body (1) connected to the temperature and humidity regulating device (3) and penetrate the side wall thereof; the mesh holes (1a) are located above the culture dish support platform (4); the temperature and humidity regulating device (3) exchanges gas with the inside of the box body (1) through the mesh holes (1a) to assist in regulating the temperature and humidity in the box body (1). The present invention has a simple structure and a high degree of automation, is suitable for cell culture operations of closed culture dishes, and effectively improves the efficiency of cell culture operations.

Description

Technical Field

[0001] The invention relates to a cell culture box, in particular to a cell culture box used for culturing adherent cells. Background Art

[0002] Cell culture is a method for studying the behavior of animal cells that was created in the early 20th century. It refers to taking cells from body tissues, simulating the in vivo growth environment, and allowing cells to grow and reproduce under sterile, appropriate temperature, pH, and nutritional conditions, while maintaining their structure and function. Cell preparation refers to the process in which researchers use relatively mature cell culture protocols to cultivate high-purity target cell products, which can be directly used in scientific research and clinical fields after passing functional and quality tests.

[0003] The operations of feeding and draining are crucial in the cell preparation process. Currently, the cell preparation process usually uses manual operation to achieve the above-mentioned feeding and draining operations, and this operation often requires repeated removal and opening of the cell culture dish to replace the internal liquid, which has a slow replacement efficiency. Moreover, traditional cell culture incubators can only provide a constant temperature and humidity environment, and do not have the function of assisting in replacing the liquid in the culture dish. In addition, during the cell culture process, the incubator needs to be opened manually to observe the growth status of the cells in the culture dish. Frequent opening of the incubator can easily cause the environment inside the box to be destroyed, which is not conducive to the full-cycle culture of cells. Summary of the invention

[0004] The object of the present invention is to provide a cell culture box for culturing adherent cells.

[0005] To achieve the above-mentioned object of the invention, the present invention provides a cell culture box for culturing adherent cells, comprising: a box body with a heating function, a box cover, a temperature and humidity adjustment device arranged on one side of the box body and located outside the box body, and a culture dish support table arranged in the box body;

[0006] The side of the box body connected to the temperature and humidity regulating device is regularly provided with mesh holes penetrating the side wall thereof;

[0007] The mesh is located above the culture dish support platform;

[0008] The temperature and humidity regulating device exchanges gas with the interior of the box through the mesh, so as to assist in regulating the temperature and humidity inside the box.

[0009] According to one aspect of the present invention, one side of the box cover is detachably rotatably connected to the upper end of the side of the box body connected to the temperature and humidity adjustment device;

[0010] An annular sealing and heat-insulating strip is provided at the upper end of the box body;

[0011] A notch for the pipeline to pass through is arranged on the side of the annular sealing and heat-insulating strip corresponding to the position where the box body and the box cover are rotatably connected.

[0012] According to one aspect of the present invention, the cross-sectional shape of the annular sealing and heat-insulating strip is semicircular or semi-elliptical;

[0013] At least one annular groove coaxial with the annular sealing and heat-insulating strip is arranged on a side of the annular sealing and heat-insulating strip away from the box body.

[0014] According to one aspect of the present invention, the box body is a hollow structure surrounded by multiple layers of plates;

[0015] The box body comprises: a heat-conducting layer, a heating layer, a heat-insulating layer, and an insulating layer arranged in sequence from the inside to the outside;

[0016] The heating layer is used to heat the heat-conducting layer, and the heat-conducting layer is used to transfer the heat generated by the heating layer to the inside of the box;

[0017] The heating layer is coated on the outside of the heat-conducting layer, the heat-insulating layer is coated on the outside of the heating layer, and the insulating layer is coated on the outside of the heating layer;

[0018] The position where the mesh is arranged on the heat-conducting layer is covered with a sterilization air permeable film;

[0019] The box cover is a hollow plate-shaped body, and one side of the box cover adjacent to the box body is made of heat-insulating material.

[0020] According to one aspect of the present invention, the culture dish support platform comprises: a connection seat supported at the bottom of the box body, a lifting seat connected to the connection seat, a driving device and a detection device for detecting the internal state of the culture dish installed on the lifting seat;

[0021] The driving device is supported on the connecting seat;

[0022] One end of the lifting seat is a rotating connection end rotatably connected to the connection seat, and the other end is a lifting connection end connected to the driving device;

[0023] Under the driving action of the driving device, the lifting connection end can perform lifting movement relative to the connecting seat.

[0024] According to one aspect of the present invention, the connection base comprises: a connection frame and a bottom support arranged at the lower side of the connection frame;

[0025] The lifting seat is located inside the connecting frame and above the bottom support;

[0026] The lifting seat is a frame structure, on which a vessel installation position is arranged;

[0027] The vessel installation position comprises: a first hollow portion and a second hollow portion that are interconnected;

[0028] The second hollow portion has an opening at the end of the rotation connection end of the lifting seat;

[0029] In the width direction of the lifting seat, the lifting seat is provided with penetrating light-transmitting channels on opposite sides of the vessel installation position;

[0030] The light-transmitting channel is arranged at a position of the first hollow part close to the lifting connection end of the lifting seat.

[0031] According to one aspect of the present invention, the driving device comprises: a driver for outputting a linear displacement, a fork arm connected to the driver;

[0032] The fork arm comprises: a driver connecting arm and a lifting seat connecting arm;

[0033] The lifting seat connecting arm is arranged perpendicular to the driver connecting arm;

[0034] The bottom support is provided with a linear channel;

[0035] One end of the lifting seat connecting arm away from the driver connecting arm passes through the linear channel and is hinged to the lifting connecting end of the lifting seat;

[0036] The lifting seat connecting arm is located at the lower side of the bottom support, and the side away from the driver connecting arm is hinged to the movable end of the driver;

[0037] A rubber damping structure is provided at the hinged position between the driver connecting arm and the driver, for silent rotation of the hinged position;

[0038] The driver connecting arm is embedded with a position sensor for detecting its moving position, and a limiting structure for limiting the maximum displacement of the driver connecting arm.

[0039] According to one aspect of the present invention, a transparent baffle and a lighting device are also provided in the box;

[0040] One side of the transparent baffle is rotatably connected to the side wall of the box body where the mesh is arranged, and the other side of the box body is provided with a supporting member for supporting the transparent baffle;

[0041] The position where the transparent baffle is connected to the side wall of the box is located above the mesh;

[0042] The shape of the transparent baffle is adapted to the inner cross-sectional shape of the box body;

[0043] The lighting device is arranged on the side wall of the box body, and the lighting device is above the culture dish supporting platform.

[0044] According to one aspect of the present invention, the transparent baffle comprises: a plurality of baffle parts slidably connected to each other; wherein, one of the baffle parts at the edge position is rotatably connected to the side wall of the box body where the mesh is arranged, and the remaining baffle parts can slide in the direction close to the opposite side of the box body, and the side edge of another baffle part at the edge position abuts against the side wall of the box body.

[0045] According to one aspect of the present invention, a convex lens portion is provided on the transparent baffle;

[0046] Anti-fog coatings are provided on opposite sides of the convex lens portion;

[0047] The edge of the transparent baffle is covered with a sealing rubber sleeve.

[0048] According to one solution of the present invention, the present invention has a simple structure and a high degree of automation, is suitable for cell culture operations in closed culture dishes, and effectively improves the efficiency of cell culture operations.

[0049] According to one solution of the present invention, the structure of the cell culture box of the present invention is simple, and the temperature and humidity in the box can be directly controlled through the mesh holes opened on the box wall by directly installing the temperature and humidity regulating device on one side. In addition, the present invention effectively shortens the ventilation path and eliminates the ventilation pipeline by directly installing the temperature and humidity regulating device on the box wall of the box body, thereby realizing accurate control of the temperature and humidity in the box body.

[0050] According to one solution of the present invention, an annular sealing strip is provided between the box body and the box cover, which can effectively eliminate the gap between the upper end surface of the box body and the box cover, thereby being beneficial to ensuring the stability of the constant temperature and humidity environment in the box body.

[0051] According to one solution of the present invention, by providing a notch on the annular sealing and heat-insulating strip, the culture dish pipeline can be installed and passed through the notch. In this way, the periphery of the culture dish pipeline can be sealed by the annular sealing and heat-insulating strip. Especially when the box cover and the box body are buckled together, the sealing strip can make the end face at the notch position contact more closely with the outer side surface of the pipeline due to the extrusion force, which is beneficial to ensure the airtightness around the pipeline. In addition, the notch on the sealing strip can also achieve the positioning of the pipeline, and no additional clamping structure is required, which effectively simplifies the installation of the pipeline in the box body.

[0052] Through the above configuration, by providing a notch on the annular sealing and heat-insulating strip, the method of opening the box body or the box cover can be conveniently and simply replaced, thereby effectively eliminating the disadvantage that the opening on the box body or the box cover causes the air tightness of the structure to deteriorate. In addition, the method of opening the sealing ring is simple and convenient and does not require the addition of an additional sealing structure, thereby effectively simplifying the structure while ensuring good air tightness.

[0053] According to one solution of the present invention, the temperature and humidity regulating device of the present invention is provided with an air purification unit, which can not only ensure the temperature and humidity in the box are within preset conditions, but also disinfect and purify the environment in the box to ensure the cleanliness of the entire box environment.

[0054] According to one solution of the present invention, by providing a transparent baffle, when the box lid is opened, the culture dishes in the box can be directly observed through the transparent baffle, thereby effectively reducing the disadvantage that the internal environment of the box is affected by the external environment when the box lid is directly opened. While facilitating the observation of the internal culture dishes, the stable operation of the internal environment can be ensured, thereby improving the efficiency and stability of the cell culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 is a perspective view schematically showing a cell culture chamber according to one embodiment of the present invention;

[0056] Figure 2 is a diagram schematically showing the internal structure of a cell culture box according to one embodiment of the present invention;

[0057] Figure 3 is a top view schematically showing a cell culture chamber according to one embodiment of the present invention;

[0058] Figure 4 is a cross-sectional view schematically showing a cell culture chamber according to one embodiment of the present invention;

[0059] Figure 5 is a structural diagram schematically showing an annular sealing heat insulating ring according to an embodiment of the present invention;

[0060] Figure 6 is a perspective view schematically showing a culture dish support table according to one embodiment of the present invention;

[0061] Figure 7 is a side view schematically showing a culture dish support table according to one embodiment of the present invention;

[0062] Figure 8 is a structural diagram schematically showing a culture dish support table according to one embodiment of the present invention;

[0063] Fig. 91 is a bottom view schematically showing a culture dish support table according to one embodiment of the present invention;

[0064] Fig.10 is a structural diagram schematically showing a lifting seat according to an embodiment of the present invention;

[0065] Fig.11 is a structural diagram schematically showing a transparent baffle according to an embodiment of the present invention;

[0066] Fig.12 is a structural diagram schematically showing a transparent baffle according to another embodiment of the present invention;

[0067] Fig.13 is a side view schematically showing a transparent baffle according to another embodiment of the present invention; DETAILED DESCRIPTION

[0068] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0069] When describing the embodiments of the present invention, the orientation or positional relationship expressed by the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" are based on the orientation or positional relationship shown in the relevant drawings and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operate in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0070] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments cannot be described one by one here, but the embodiments of the present invention are not therefore limited to the following embodiments.

[0071] Combination Figure 1 , Figure 2 , Figure 3 , Figure 4As shown, according to one embodiment of the present invention, a cell culture box with a microscopic imaging system of the present invention comprises: a box body 1, a box cover 2, a temperature and humidity adjustment device 3 arranged on one side of the box body 1 and located outside the box body 1, and a culture dish support table 4 arranged in the box body 1. In this embodiment, the box body 1 is a symmetrical rectangular structure as a whole. Meshes 1a are regularly arranged on one side of the box body 1 connected to the temperature and humidity adjustment device 3 and passing through its side wall. In this embodiment, the meshes 1a are distributed in an array symmetrically on both sides of the center line perpendicular to the length direction of the box body. In this embodiment, the meshes 1a are located above the culture dish support table 4. In this embodiment, the temperature and humidity adjustment device 3 exchanges gas with the interior of the box body 1 through the meshes 1a to assist in adjusting the temperature and humidity in the box body 1.

[0072] In this embodiment, the box 1 has a heating function, which can heat the internal environment to ensure that the internal environment of the box has a suitable cell culture temperature. When the temperature inside the box is high, the heating of the box 1 can be stopped and / or the temperature inside the box can be adjusted by operating the temperature and humidity adjustment device 3, so that the temperature of the internal environment of the box is always kept within a reasonable range.

[0073] In this embodiment, when the humidity of the environment inside the box needs to be controlled, the temperature and humidity regulating device 3 can be operated alone to humidify or dehumidify the environment inside the box to keep the humidity of the environment inside the box constant.

[0074] In this embodiment, the number and size of the mesh holes 1a on the box body 1 are matched with the volume of the box body. The mesh holes 1a arranged as above can realize the circulation of air in the box body 1 by the temperature and humidity regulating device 3 without generating obvious airflow in the box body, especially in combination with the mesh holes 1a being opened in the upper half of the side wall of the box body 1 (i.e., above the culture dish support platform 4), which can more effectively eliminate the flow of gas in the box body and realize the stable exchange of gas.

[0075] Through the above arrangement, the cell culture box of the present invention has a simple structure, and the temperature and humidity in the box can be directly controlled through the mesh holes opened on the box wall by directly installing the temperature and humidity regulating device 3 on one side. In addition, the present invention effectively shortens the ventilation path and eliminates the ventilation pipeline by directly installing the temperature and humidity regulating device 3 on the box wall of the box body 1, thereby realizing accurate control of the temperature and humidity in the box body 1.

[0076] Combination Figure 1 , Figure 2 , Figure 3 , Figure 4As shown, according to an embodiment of the present invention, one side of the box cover 2 is detachably rotatably connected to the upper end of the side of the box body 1 connected to the temperature and humidity adjustment device 3. In this embodiment, the box cover 2 is connected to the box body 1 through a detachable pin shaft. When installing a culture dish and other structures into the box body 1, the box cover 2 can be detached from the box body 1. After the culture dish is installed, the box cover 2 is connected to the box body 1 to achieve sealing of the box body 1. In this embodiment, the lighting device 6 is installed on the inner side of the box cover 2. Among them, the lighting device 6 is detachably connected to the box cover 2.

[0077] Combination Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 As shown, according to one embodiment of the present invention, an annular sealing and heat-insulating strip 11 is provided at the upper end of the box body 1. In this embodiment, the cross-sectional shape of the annular sealing and heat-insulating strip 11 is semicircular or semi-elliptical. By setting the upper side of the annular sealing and heat-insulating strip 11 to be arc-shaped, it can achieve line contact with the box cover, and effectively make close contact with each position of the box cover.

[0078] Through the above configuration, the annular sealing strip provided between the box body 1 and the box cover 2 can effectively eliminate the gap between the upper end surface of the box body and the box cover, thereby being beneficial to ensuring the stability of the constant temperature and humidity environment in the box body.

[0079] In this embodiment, at least one annular groove 112 coaxial with the annular sealing and heat-insulating strip 11 is provided on the side of the annular sealing and heat-insulating strip 11 away from the box body 1. In this embodiment, the cross section of the annular groove 112 is rectangular. Then the side of the annular groove 112 is a vertical surface, and then a sharp angle is formed at the connection position between the upper side of the annular sealing and heat-insulating strip and the curved surface, and then the position where it contacts the box cover 2 can achieve a multiple sealing effect. In addition, the strength of the sharp-angled edge formed by the annular groove on the sealing strip is relatively small, and then a slight bend can be generated toward the inside of the groove at the position in contact with the box cover, so that a closer contact with the box cover can be achieved, and when the edge is slightly bent, a small distance of relative sliding can be generated between the box cover, which can produce a cleaning effect on the position in contact with the box cover, and ensure the sealing of the contact position. In addition, a plurality of annular grooves 112 can be arranged in parallel at intervals in the width direction of the annular sealing and heat-insulating strip 11, so that a plurality of edges of different heights can be formed on the surface of the sealing strip to achieve a multiple sealing effect.

[0080] In this embodiment, a notch 111 for the pipeline to pass through is provided on the side corresponding to the position where the annular sealing and heat-insulating strip 11 is rotatably connected to the box body 1 and the box cover 2. In this embodiment, the width and shape of the notch match the cross-sectional shape of the pipeline passing through.

[0081] Through the above arrangement, by providing the notch 111 on the annular sealing and heat-insulating strip 11, the culture dish pipeline can be installed and passed through the notch. In this way, the periphery of the culture dish pipeline can be sealed through the annular sealing and heat-insulating strip 11. Especially when the box cover and the box body are buckled together, the sealing strip can make the end face at the position of the notch 111 contact the outer side of the pipeline more closely due to the extrusion force, which is beneficial to ensure the air tightness around the pipeline. In addition, the notch 11 on the sealing strip can also achieve the positioning effect of the pipeline, and no additional clamping structure is required, which effectively simplifies the installation of the pipeline in the box body 1.

[0082] Through the above configuration, by providing the notch 111 on the annular sealing and heat-insulating strip 11, the method of opening the box body or the box cover can be conveniently and simply replaced, thereby effectively eliminating the disadvantage that the opening on the box body or the box cover causes the air tightness of the structure to deteriorate. In addition, the method of opening the sealing ring is simple and convenient and does not require the addition of an additional sealing structure, thereby effectively simplifying the structure while ensuring good air tightness.

[0083] Combination Figure 1 , Figure 2 , Figure 3 , Figure 4 As shown, according to one embodiment of the present invention, the box body 1 is a hollow structure surrounded by a multi-layer plate body. In this embodiment, the box body 1 includes: a heat-conducting layer, a heating layer, a thermal insulation layer, and an insulating layer arranged in sequence from the inside to the outside. In this embodiment, the heat-conducting layer is made of a stainless steel metal plate. The heating layer is used to heat the heat-conducting layer. The heat-conducting layer made of metal is convenient for transferring the heat generated by the heating layer to the inside of the box body 1, thereby performing constant temperature control on the inside of the box body. In this embodiment, the heating layer is coated on the outside of the heat-conducting layer, the thermal insulation layer is coated on the outside of the heating layer, and the insulating layer is coated on the outside of the heating layer; the heating layer is effectively used to suppress the loss of heat generated by the heating device, which is beneficial to accurately control the constant temperature effect of the box body. In addition, the insulating layer is also effectively guaranteed to ensure the safety of the box body 1.

[0084] In this embodiment, the position where the mesh 1a is set on the heat conductive layer is covered with a sterilization air-permeable membrane; the sterilization air-permeable membrane can be used to isolate the inside of the box from the external environment. When the gas passes through the sterilization air-permeable membrane, it not only purifies the gas, but also is beneficial to suppress the airflow generated by gas exchange.

[0085] By setting the box body as a multi-layer composite structure, its structure is simple and can be easily embedded in other systems, ensuring the flexibility of use and installation of the present invention. In addition, by setting the box body with a heating layer, it is convenient to achieve direct heating inside the box body, eliminating the intermediate heat exchange path, effectively reducing contact with the outside world, and facilitating accurate control of the temperature inside the box body. At the same time, since the temperature and humidity adjustment device 3 is directly connected to the box body, by setting a heat insulation layer and an insulating layer on the box body, the influence of the heat generated during the operation of the temperature and humidity adjustment device 3 on the internal environment of the box body can also be effectively eliminated.

[0086] In this embodiment, the box cover 2 is a hollow plate-shaped body, and the side thereof adjacent to the box body 1 is made of a heat insulating material.

[0087] Through the above arrangement, the cooperation with the box body 1 is effectively achieved, which is beneficial to ensuring the stability of the environment inside the box.

[0088] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, according to one embodiment of the present invention, the culture dish support platform 4 includes: a connection seat 41 supported at the bottom of the box body 1, a lifting seat 42 connected to the connection seat 41, a driving device 43 and a detection device 44 for detecting the internal state of the culture dish installed on the lifting seat 42. In this embodiment, the driving device 43 is supported on the connection seat 41. One end of the lifting seat 42 is a rotating connection end rotatably connected to the connection seat 41, and the other end thereof is a lifting connection end connected to the driving device 43. In this embodiment, under the driving action of the driving device 43, the lifting connection end can perform lifting motion relative to the connection seat 41.

[0089] Through the above arrangement, the lifting seat 42 in the present invention is used to carry a culture dish (for example, an adherent cell culture dish), and then the lifting connection end of the lifting seat can be realized by the action of the driving device to rotate the connection end to perform a vertical lifting action, so that the lifting seat 42 produces a tilting movement relative to the connecting seat 41, thereby promoting the liquid in the culture dish to flow out smoothly when it needs to be replaced, avoiding the generation of residue in the culture dish.

[0090] Through the above arrangement, the present invention can automatically realize the lifting and lowering operation of the lifting seat without manual participation, and can effectively improve the renewal efficiency of the liquid in the culture dish.

[0091] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9As shown, according to one embodiment of the present invention, the connection seat 41 includes: a connection frame 411 and a bottom support 412 arranged at the lower side of the connection frame 411. In this embodiment, the connection frame 411 is a continuous rectangular frame, and its size can be adaptively adjusted according to the size of the interior of the box. In this embodiment, the bottom support 412 is a plate-like body, and it is fixedly installed at the lower side of the connection frame 411. In this embodiment, the bottom support 412 and the connection frame 411 can be integrally arranged, or can be fixed by bonding, threaded connection, etc.

[0092] In this embodiment, a plurality of legs are provided at intervals on the lower side of the connection frame 411. The height of the legs can be adjusted as needed. In this embodiment, the connection frame 411 is mounted on the bottom of the box 1 through the legs. In this embodiment, the legs can be fixed to the bottom of the box 1 through a connector to achieve fixed installation of the culture dish support 4.

[0093] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, according to an embodiment of the present invention, the lifting seat 42 is located inside the connection frame 411 and above the bottom support 412. In this embodiment, the two opposite sides of the rotation connection end of the lifting seat 42 are respectively provided with a rotating shaft 42a for rotationally connecting with the connection frame 411. The rotating shaft 42a is connected by bearings installed on the lifting seat 42 and the connection frame 411, so as to realize the flexible rotation of the lifting seat 42 relative to the connection frame 411. In this embodiment, in order to realize the connection of the lifting seat 42 inside the connection frame 411, the outer shape of the lifting seat 42 is adapted to the inner shape of the connection frame 411. If the inner side of the connection frame 411 is rectangular, the outer shape of the lifting seat 42 should be set to be rectangular to match it.

[0094] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 , Fig.10As shown, according to one embodiment of the present invention, the lifting seat 42 is a frame structure, which has a vessel mounting position 42b for mounting a culture dish. In this embodiment, the lifting seat 42 is a hollow frame structure, and the vessel mounting position 42b for the culture dish thereon is formed by a hollow structure on the lifting seat 42. Specifically, the vessel mounting position 42b includes: a first hollow portion 42b1 and a second hollow portion 42b2 that are interconnected. In this embodiment, along the direction from the rotating connection end to the lifting connection end of the lifting seat 42 (i.e., the length direction), the second hollow portion 42b2 and the first hollow portion 42b1 are arranged in sequence, wherein the second hollow portion 42b2 has an opening at the end of the rotating connection end of the lifting seat 42. In this embodiment, the widths of the first hollow portion 42b1 and the second hollow portion 42b2 are different, thereby forming a step structure on the vessel mounting position 42b, which is beneficial to the installation and positioning of the culture dish in the first hollow portion 42b1, ensuring the installation stability of the culture dish, while the second hollow portion 42b2 corresponds to the bottleneck and bottle cap structures on the culture dish, thereby achieving stable installation of the entire culture dish.

[0095] In this embodiment, the second hollow portion 42b2 forms an opening at the end of the rotating connection end, which can further avoid interference with structures such as bottlenecks and bottle caps on the culture dish, can adapt to the installation of bottlenecks and bottle caps of different sizes, and during the lifting process of the lifting seat 42, provides sufficient space to avoid interference between the entire culture dish support platform 4 and the culture dish.

[0096] Through the above arrangement, the vessel installation position 42b is set to a hollow structure, which can effectively reduce the position where there is interference with the culture dish, which is conducive to the stable installation of the culture dish. In addition, by setting the vessel installation position 42b to be hollow, the mass of the lifting seat can be greatly reduced, and the structure of the lifting seat can be simplified, which is beneficial to ensuring the long-term stable operation of the present invention. In addition, by setting the vessel installation position 42b to be hollow, it is also conducive to installing other optical equipment on the culture dish support platform 4 of the present invention for real-time observation of the state in the culture dish, which effectively improves the flexibility of use of the present invention.

[0097] In this embodiment, an arc surface is provided at the lower side of the end of the rotating connection end of the lifting seat 42, and the arc surface is used to connect the end surface and the lower side surface of the rotating connection end, thereby forming a transition part at the end position.

[0098] Through the above arrangement, an arc surface is provided on the lower side of the end of the rotating connection end of the lifting seat 42, which is conducive to ensuring the smooth rotation of the lifting seat 42 and further ensuring the stability of the use of the present invention.

[0099] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, according to one embodiment of the present invention, in the width direction of the lifting seat 42, the lifting seat 42 is provided with penetrating light-transmitting channels 421 on opposite sides of the vessel installation position 42b; in this embodiment, the light-transmitting channels 421 are provided at a position close to the lifting connection end of the lifting seat 42 in the first hollow part 42b1. In this embodiment, the light-transmitting channels 421 are coaxially provided on the frame of the lifting seat 42, and then, when the culture dish is fixed, the light-transmitting channels 421 can be coaxially provided on opposite sides of the culture dish, and then, light can be illuminated through the light-transmitting channels 421 on one side, and can pass through the culture dish and reach the light-transmitting channels 421 on the other side and be transmitted out, and then the internal state of the culture dish (such as the internal environment, cell growth state, pollution condition, turbidity of the nutrient solution, etc.) can be collected for subsequent detection and analysis.

[0100] In this embodiment, in the thickness direction of the lifting seat 42, the light-transmitting channel 421 is arranged near the lower side of the lifting seat 42, that is, the distance between the light-transmitting channel 421 and the lower side is smaller than the distance between the connecting channel and the upper side.

[0101] Through the above arrangement, by arranging the light-transmitting channel 421 at a position close to the lifting connection end of the lifting seat 42, it can be used to perform real-time detection of the internal environment of the rear of the culture dish, so as to accurately detect the internal environment of the culture dish.

[0102] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 , Fig.10 As shown, according to one embodiment of the present invention, a support structure 422 is provided at the bottom of the lifting seat 42. In this embodiment, the support structure 422 includes: a first support structure 4221 and a second support structure 4222. The first support structure 4221 is provided along the inner edge of the bottom of the first hollow part 42b1; the second support structure 4222 is connected to the inner edges of the bottom of the second hollow part 42b2 on both sides. In this embodiment, the first support structure 4221 is a long strip structure, which is continuously or discontinuously provided at the inner edge of the bottom of the first hollow part 42b1. In this embodiment, the second support structure 4222 is a plate-like body, and its opposite ends are fixedly connected to the inner edges of the bottom of the second hollow part 42b2 on both sides.

[0103] Through the above arrangement, by providing the support structure 422 at the bottom edge of the vessel mounting position 42b, effective support can be provided for the edge and bottleneck of the culture vessel.

[0104] In this embodiment, the second supporting structure 4222 is spaced apart from the end opening of the rotating connection end.

[0105] Through the above arrangement, the interval between the second supporting structure 4222 and the end opening of the rotating connection end can play an avoidance role, avoiding interference with other structures, which is beneficial to ensuring the normal and stable operation of the present invention.

[0106] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, according to one embodiment of the present invention, the driving device 43 includes: a driver 431 for outputting linear displacement, and a fork arm 432 connected to the driver 431. In this embodiment, the fork arm 432 includes: a driver connecting arm 4321 and a lifting seat connecting arm 4322; the lifting seat connecting arm 4322 is arranged perpendicular to the driver connecting arm 4321. In this embodiment, the driver connecting arm 4321 and the lifting seat connecting arm 4322 can be arranged as one piece, or can be connected by threading, bonding, riveting, etc. In this embodiment, two lifting seat connecting arms 4322 are arranged, which are connected to the driver connecting arm 4321 in parallel.

[0107] Combination Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, according to an embodiment of the present invention, a linear channel 4121 is provided on the bottom support 412. In this embodiment, one end of the lifting seat connecting arm 4322 away from the driver connecting arm 4321 passes through the linear channel 4121 and is hinged to the lifting connection end of the lifting seat 42. In this embodiment, a hinge seat for connecting with the driver connecting arm 4321 is provided at the lower side of the lifting connection end. The hinge seat is composed of two parallel and spaced plate-like bodies, and the connection can be completed by the hinge shaft passing through the corresponding installation positions on the hinge seat and the driver connecting arm 4321 in sequence.

[0108] In this embodiment, the lifting seat connecting arm 4322 is located at the lower side of the bottom support 412, and the side away from the driver connecting arm 4321 is hinged to the movable end of the driver 431. In this embodiment, a first notch is provided on the bottom support 412 below the rotating connection end of the lifting seat 42, and a second notch is provided at a position of the connecting frame 411 of the connecting seat 41 corresponding to the first notch, and the second notch is connected to the first notch, so that the driving device 43 can be installed at the position of the first notch and the second notch.

[0109] In this embodiment, the driver 431 is a linear driving device, and its telescopic end can telescope along a linear direction, and then the free end of the driver 431 is hinged to the driver connecting arm 4321 to achieve the pushing effect on the lifting seat 42.

[0110] According to one embodiment of the present invention, the driver 431 is implemented by an electric cylinder, which can accurately control the displacement of its telescopic end through an electrical signal. The inclination angle of the lifting seat can be automatically and accurately controlled through the control of the electrical signal, thereby achieving a small flow of nutrient solution in the culture dish and flexible adjustment of the horizontal position of the nutrient solution.

[0111] Through the above arrangement, the driver connecting arm 4321 is arranged at the lower side of the bottom support 412, and the lifting seat 42 is located at the upper side of the bottom support 412. In the process of driving the lifting seat 42 to move up and down through the connection between the driver 431 and the driver connecting arm 4321, the bottom support 412 can support and limit the driver connecting arm 4321, thereby facilitating the stable movement of the driver 431. In addition, through the above arrangement, the accurate control of the tilt angle of the lifting seat 42 can be flexibly achieved by controlling the telescopic length of the free end of the driver 431, without the need for other complex structures, achieving the advantages of high operating accuracy, good stability and simple structure.

[0112] In this embodiment, a rubber damping structure is provided at the hinged position between the driver connecting arm 4321 and the driver 431 for silent rotation of the hinged position. In this embodiment, a rubber damping structure can be provided on both sides of the hinged position or on the hinged shaft to achieve silencing of the hinged position, and also effectively ensure smooth and stable operation and accurate operation position.

[0113] In this embodiment, the driver connecting arm 4321 is embedded with a position sensor for detecting its moving position. The real-time detection of the position of the driver connecting arm 4321 by the position sensor can realize accurate control of the position of the fork arm 32, thereby realizing accurate control of the tilt angle of the lifting seat.

[0114] In this embodiment, a limiting structure for limiting the maximum displacement of the driver connecting arm 4321 is also provided on the driver connecting arm 4321. The limiting structure can effectively avoid excessive movement of the driver connecting arm 4321, thereby effectively suppressing the maximum lifting height of the lifting seat 42, thereby ensuring the stable operation of the lifting seat and eliminating interference with other structures.

[0115] Combination Figure 6 , Figure 7As shown, according to one embodiment of the present invention, a detection device 44 for detecting the internal state of the culture dish installed on the lifting seat 42 is provided on the connecting frame 411. In this embodiment, the detection device adopts a cross-beam detection device. Specifically, the detection device 44 includes: a laser light source 441, an optical fiber 442, and an optical receiver 443. In this embodiment, mounting through holes penetrating the frame are respectively provided on opposite sides of the connecting frame 411, so that the laser light source 441 and the optical receiver 443 can be relatively installed in the mounting through holes. The optical fiber 442 is respectively embedded in the light-transmitting channel 421 of the lifting seat 42; when the lifting seat 42 is horizontally located in the connecting frame 411, the laser light source 441, the optical fiber 442, and the optical receiver 443 are aligned, and the light emitted by the laser light source 441 can be transmitted to the optical receiver 443 through the optical fiber 442, thereby realizing the detection of the environment in the culture dish. When the lifting seat 42 is tilted, the lifting connection end leaves the connecting frame 411, and the detection device 44 can output the lifting seat 42 lifting signal, and the environment in the culture dish cannot be detected. In this embodiment, the optical receiver 443 can process and analyze the received signal by itself, or transmit it to other devices for processing and analysis, which can be set according to the actual application.

[0116] Through the above arrangement, the automatic detection of the interior of the culture dish can be realized by using the detection device to detect the culture dish. In addition, the automatic control of the lifting seat can also be realized by the on-off of the detection device, thereby improving the control accuracy of the present invention.

[0117] In addition, the optical fiber 442 can be set against the culture dish as needed, which can provide a certain pre-tightening effect on the culture dish, which is beneficial for the reliable fixation of the culture dish. Moreover, this setting method is more beneficial when the lifting seat is in an inclined state. By the pre-tightening effect on the rear of the culture dish, the problem of the liquid in the culture dish gathering at the bottleneck end and causing the culture dish to tip over can be effectively avoided, making the operation of the present invention more reliable. In addition, the optical fiber 442 can be set as a whole with the culture dish to eliminate the influence of the contact position on the detection result, and further play a role in fixing and connecting the culture dish.

[0118] Combination Figure 3 , Figure 4 As shown, according to an embodiment of the present invention, a transparent baffle 12 and an illuminating device 13 are further provided in the box body 1. In this embodiment, one side of the transparent baffle 12 is rotatably connected to the side wall of the box body 1 where the mesh 1a is provided, and a supporting member 14 for supporting the transparent baffle 12 is provided on the other side of the box body 1. In this embodiment, the transparent baffle 12 is rotatably connected to the side wall of the box body 1 so that a transparent cover that can be opened and closed is provided in the box body 1.

[0119] Through the above-mentioned configuration, by setting the transparent baffle 12, when the box cover 2 is opened, the culture dish in the box can be directly observed through the transparent baffle 12, thereby effectively reducing the disadvantage that the internal environment of the box is affected by the external environment when the box cover 2 is directly opened. While facilitating the observation of the internal culture dish, the stable operation of the internal environment can be ensured, thereby improving the high efficiency and high stability in the cell culture process.

[0120] In this embodiment, the support member 14 is in the shape of a long strip, which can be arranged at intervals or continuously on the other inner side surfaces of the box body 1 (i.e., except the side where the transparent baffle 12 is rotatably connected to the box body), so that the transparent baffle 12 can be supported on the support member 14 after being lowered. In this embodiment, a flexible material is attached to the support member 14, which can provide better buffering and sealing when in contact with the transparent baffle 12.

[0121] In this embodiment, the transparent baffle 12 can be made of transparent materials such as resin and glass.

[0122] In this embodiment, the position where the transparent baffle 12 is connected to the side wall of the box body 1 is located above the mesh 1a; the shape of the transparent baffle 12 is adapted to the inner cross-sectional shape of the box body 1.

[0123] Through the above arrangement, good sealing performance of the transparent baffle 12 to the box body 1 is ensured.

[0124] In this embodiment, the lighting device 13 is disposed on the side wall of the housing 1 , and the lighting device 13 is above the culture dish support 4 .

[0125] By providing the lighting device 13, the interior of the box body 1 can be illuminated to facilitate observation of the interior of the culture dish, while also reducing the disadvantage that the internal environment is affected by the external environment.

[0126] In this embodiment, the lighting device 13 can be an LED light strip, which can be circumferentially arranged along the inner wall of the box body 1 so that the culture dish can be illuminated in all directions, making it convenient to observe various parts inside the culture dish.

[0127] Combination Figure 3 , Figure 4 , Fig.11 , Fig.12 , Fig.13 As shown, according to one embodiment of the present invention, the transparent baffle 12 can be a whole structure (such as Fig.11 ), or it can be a composite structure composed of parts (such as Fig.12 , Fig.13If the transparent baffle 12 is a combined structure composed of parts, the transparent baffle 12 includes: a plurality of baffle parts 121 slidably connected to each other; wherein one baffle part 121 at an edge position is rotatably connected to the side wall of the box body 1 where the mesh 1a is provided, and the remaining baffle parts 121 can slide toward the opposite side of the box body 1, and the side edge of another baffle part 121 at an edge position abuts against the side wall of the box body 1.

[0128] Through the above arrangement, the transparent baffle 12 is arranged as a multi-part sliding combination structure, which is more convenient to store and occupies less space in the stored state, and is also more convenient to unfold, effectively avoiding interference with other structures.

[0129] According to an embodiment of the present invention, a magnetic attraction device may be provided on the edge of the transparent baffle 12 , and the magnetic attraction device and the support member 14 may be attracted to each other to effectively ensure the closure stability of the transparent baffle.

[0130] Combination Fig.11 , Fig.12 , Fig.13 As shown, according to one embodiment of the present invention, a convex lens portion 122 is provided on the transparent baffle 12. In this embodiment, anti-fog coatings are provided on opposite sides of the convex lens portion 122. In this embodiment, the convex lens portion 122 is provided above the vessel mounting position 42b, which can facilitate observation of the cell culture dish installed on the vessel mounting position 42b. In this embodiment, the convex lens portion 122 can be provided with a single-sided protrusion or a double-sided protrusion, but in the state where the transparent baffle 12 is provided with multiple parts, it is necessary to set the convex lens portion at a position that does not affect the sliding of each part, or to set an avoidance structure at the corresponding position of each part that slides with each other to eliminate their interference.

[0131] Through the above configuration, the anti-fog layer is provided on the surface of the convex lens portion 122 to effectively eliminate the fogging phenomenon caused by the temperature difference between the inside and the outside, thereby more effectively ensuring the perspective effect.

[0132] Combination Fig.11 , Fig.12 , Fig.13 As shown, according to an embodiment of the present invention, the edge of the transparent baffle 12 is covered with a sealing rubber sleeve 123. The above arrangement further ensures the sealing of the contact position between the transparent baffle 12 and the inner side of the box body.

[0133] According to one embodiment of the present invention, the temperature and humidity adjustment device 3 includes: an air conditioning unit and an air purification unit. In this embodiment, the temperature and humidity of the environment inside the box 1 are auxiliary adjusted by the air conditioning unit, and the environment inside the box is further disinfected and purified by the air purification unit to further ensure the cleanliness of the entire box environment.

[0134] According to one embodiment of the present invention, a carbon dioxide sensor, an oxygen sensor, a humidity sensor, and a temperature sensor are provided in the box 21 for real-time detection of the internal environment of the box 21, and then the real-time and accurate adjustment of the internal environment of the box can be achieved through the temperature and humidity adjustment device 23 and the heating layer of the box, thereby ensuring the balance of temperature and humidity in the box and avoiding gradient differences in the box.

[0135] The above contents are merely examples of specific solutions of the present invention. For devices and structures not described in detail therein, it should be understood that they can be implemented by adopting general devices and general methods available in the art.

[0136] The above is only one solution of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture incubator for culturing adherent cells, characterized in that: include: A box body (1) having a heating function, a box cover (2), a temperature and humidity adjustment device (3) arranged on one side of the box body (1) and located outside the box body (1), and a culture dish support table (4) arranged inside the box body (1); A side of the box (1) connected to the temperature and humidity regulating device (3) is provided with mesh holes (1a) regularly arranged and penetrating its side wall; The mesh (1a) is located above the culture dish support platform (4); The temperature and humidity regulating device (3) exchanges gas with the interior of the box (1) through the mesh (1a), so as to assist in regulating the temperature and humidity inside the box (1); The culture dish support platform (4) comprises: a connecting seat (41) supported at the bottom of the box body (1), a lifting seat (42) connected to the connecting seat (41), a driving device (43) and a detection device (44) for detecting the internal state of the culture dish installed on the lifting seat (42); The driving device (43) is supported on the connecting seat (41); One end of the lifting seat (42) is a rotating connection end rotatably connected to the connection seat (41), and the other end is a lifting connection end connected to the driving device (43); Under the driving action of the driving device (43), the lifting connection end can perform lifting movement relative to the connecting seat (41); The connecting seat (41) comprises: a connecting frame (411) and a bottom support (412) arranged at the lower side of the connecting frame (411); The lifting seat (42) is located inside the connecting frame (411) and above the bottom support (412); The lifting seat (42) is a frame structure, on which a vessel mounting position (42b) is arranged; The vessel installation position (42b) comprises: a first hollow part (42b1) and a second hollow part (42b2) which are interconnected; The second hollow portion (42b2) has an opening at the end of the rotation connection end of the lifting seat (42); In the width direction of the lifting seat (42), the lifting seat (42) is provided with penetrating light-transmitting channels (421) on opposite sides of the vessel installation position (42b); The light-transmitting channel (421) is arranged at a position of the first hollow portion (42b1) close to the lifting connection end of the lifting seat (42); The detection device (44) is a beam detection device; The detection device (44) comprises: a laser light source (441), an optical fiber (442), and an optical receiver (443); On opposite sides of the connection frame (411), mounting through holes penetrating the connection frame (411) are respectively provided, and the laser light source (441) and the optical receiver (443) are mounted in the mounting through holes in a relative manner; The optical fibers (442) are respectively embedded in the light-transmitting channels (421) of the lifting seats (42); When the lifting seat (42) is horizontally located in the connecting frame (411), the laser light source (441), the optical fiber (442), and the optical receiver (443) are aligned, and the light emitted by the laser light source (441) can be transmitted to the optical receiver (443) through the optical fiber (442) to detect the environment in the culture dish.

2. The cell culture incubator according to claim 1, characterized in that: One side of the box cover (2) is detachably rotatably connected to the upper end of the side of the box body (1) connected to the temperature and humidity adjustment device (3); An annular sealing and heat-insulating strip (11) is provided at the upper end of the box body (1); A notch (111) for the pipeline to pass through is provided on the side of the annular sealing heat insulation strip (11) corresponding to the position where the box body (1) and the box cover (2) are rotatably connected.

3. The cell culture incubator according to claim 2, characterized in that: The cross-sectional shape of the annular sealing and heat-insulating strip (11) is semicircular or semi-elliptical; At least one annular groove (112) coaxial with the annular sealing and heat-insulating strip (11) is provided on a side of the annular sealing and heat-insulating strip (11) away from the box body (1).

4. The cell culture incubator according to claim 3, characterized in that: The box body (1) is a hollow structure surrounded by multiple layers of plates; The box body (1) comprises: a heat-conducting layer, a heating layer, a heat-insulating layer, and an insulating layer which are arranged in sequence from the inside to the outside; The heating layer is used to heat the heat-conducting layer, and the heat-conducting layer is used to transfer the heat generated by the heating layer to the interior of the box (1); The heating layer is coated on the outside of the heat-conducting layer, the heat-insulating layer is coated on the outside of the heating layer, and the insulating layer is coated on the outside of the heating layer; The position where the mesh hole (1a) is arranged on the heat conductive layer is covered with a sterilization air permeable film; The box cover (2) is a hollow plate-shaped body, and the side thereof adjacent to the box body (1) is made of a heat-insulating material.

5. The cell culture incubator according to claim 4, characterized in that: The driving device (43) comprises: a driver (431) for outputting a linear displacement, and a fork arm (432) connected to the driver (431); The fork arm (432) comprises: a driver connecting arm (4321) and a lifting seat connecting arm (4322); The lifting seat connecting arm (4322) is arranged perpendicular to the driver connecting arm (4321); The bottom support (412) is provided with a linear channel (4121); One end of the lifting seat connecting arm (4322) away from the driver connecting arm (4321) passes through the linear channel (4121) and is hingedly connected to the lifting connection end of the lifting seat (42); The lifting seat connecting arm (4322) is located at the lower side of the bottom support (412), and the side away from the driver connecting arm (4321) is hinged to the movable end of the driver (431); A rubber damping structure is provided at the hinged position between the driver connecting arm (4321) and the driver (431) for silent rotation of the hinged position; The driver connecting arm (4321) is embedded with a position sensor for detecting its moving position, and a limit structure for limiting the maximum displacement of the driver connecting arm (4321).

6. The cell culture incubator according to claim 5, characterized in that: A transparent baffle (12) and a lighting device (13) are also provided in the box body (1); One side of the transparent baffle (12) is rotatably connected to a side wall of the box (1) on which the mesh (1a) is arranged, and a support member (14) for supporting the transparent baffle (12) is arranged on the other side of the box (1); The position where the transparent baffle (12) is connected to the side wall of the box body (1) is located above the mesh hole (1a); The transparent baffle (12) is configured such that its outer shape matches the inner cross-sectional shape of the box body (1); The lighting device (13) is arranged on a side wall of the box body (1), and the lighting device (13) is above the culture dish support platform (4).

7. The cell culture incubator according to claim 6, characterized in that: The transparent baffle (12) comprises: a plurality of baffle parts (121) slidably connected to each other; wherein one of the baffle parts (121) at an edge position is rotatably connected to a side wall of the box (1) on which the mesh (1a) is arranged, and the remaining baffle parts (121) can slide in a direction close to the opposite side of the box (1), and the side edge of another baffle part (121) at an edge position abuts against the side wall of the box (1).

8. The cell culture incubator according to claim 6 or 7, characterized in that: The transparent baffle (12) is provided with a convex lens portion (122); Anti-fog coatings are provided on opposite sides of the convex lens portion (122); The edge of the transparent baffle (12) is covered with a sealing rubber sleeve (123).

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