A non-direct contact ultrasonic nucleic acid extraction instrument

Abstract

The invention discloses a non-direct contact ultrasonic nucleic acid extractor, which comprises a base, an ultrasonic component mounted on the base, a fixed platform located above the ultrasonic component, and a fixing device fixed on the fixed platform and used to fix a reagent box, wherein the ultrasonic component comprises a first translation mechanism fixed on the surface of the base, a carrier plate mounted on the first translation mechanism, a lifting mechanism fixed on the carrier plate, an ultrasonic generator fixed on the lifting mechanism, and a vibration head fixed on the output end of the ultrasonic generator, a plurality of profiling grooves are arranged along the length direction of the outer end surface of the vibration head, the plurality of profiling grooves are matched with the bottom of the reagent box, a narrow groove is also arranged along the length direction of the outer end surface of the vibration head, the narrow groove runs through the central area of ​​the plurality of profiling grooves, and the depth of the narrow groove is greater than the depth of the profiling groove. The invention has a simple structure, small footprint, high stability, low noise, high mixing efficiency, fast heating speed, and no cross infection.

Description

Technical Field

[0001] The invention relates to the technical field of nucleic acid extraction, and in particular to a non-direct contact ultrasonic nucleic acid extractor. Background Art

[0002] Nucleic acid is one of the most basic substances of life. It is widely present in all animal and plant cells and microorganisms, and plays an important role in the life activities of organisms. If there is a slight change in its structure, it may lead to mutations, defects or diseases. In order to study nucleic acid, it needs to be extracted, so an extraction device is needed to extract nucleic acid.

[0003] At present, the extraction method of non-direct contact ultrasonic nucleic acid extractors on the market is usually just to use a motor to drive the oscillation module to oscillate the sample up and down at a high frequency to achieve lysis. For example, the Chinese application number is 202011024620.0, which is a high-efficiency extraction oscillation module of a non-direct contact ultrasonic nucleic acid extractor and a non-direct contact ultrasonic nucleic acid extractor. This type of oscillation method uses strong mechanical oscillation and heating to mix the sample, thereby releasing the nucleic acid. During this oscillation process, the noise is high due to excessive load. However, if the noise is reduced, the oscillation frequency will be reduced, resulting in a significant reduction in the mixing efficiency.

[0004] Therefore, it is necessary to provide a new non-direct contact ultrasonic nucleic acid extractor to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to solve the problems of low mixing efficiency and high noise in the prior art, and to propose a non-direct contact ultrasonic nucleic acid extractor.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a non-direct contact ultrasonic nucleic acid extractor, which includes a base, an ultrasonic component installed on the base, a fixed platform located above the ultrasonic component and a fixing device fixed on the fixed platform and used to fix a reagent box, the ultrasonic component includes a first translation mechanism fixed to the surface of the base, a carrier plate installed on the first translation mechanism, a lifting mechanism fixed on the carrier plate, an ultrasonic generator fixed on the lifting mechanism and a vibration head fixed to the output end of the ultrasonic generator, a plurality of profiling grooves are provided along the length direction of the outer end surface of the vibration head, and the plurality of profiling grooves are adapted to the bottom of the reagent box, and a narrow groove is also provided along the length direction of the outer end surface of the vibration head, the narrow groove runs through the central area of ​​the plurality of profiling grooves, and the depth of the narrow groove is greater than the depth of the profiling groove.

[0007] The lower section of the narrow groove forms a bottom groove, the maximum width of the bottom groove is greater than the width of the upper section of the narrow groove, and the cross-section of the bottom groove is circular, rectangular, square, trapezoidal or triangular.

[0008] The vibration head comprises a vibration head lower part and a vibration head upper part integrally formed with the vibration head lower part, and a plurality of contoured grooves are formed inwardly from the outer end surface of the vibration head upper part.

[0009] The thickness of the lower part of the vibration head is greater than the thickness of the upper part of the vibration head, and the connection between the lower part of the vibration head and the upper part of the vibration head is transitioned through an arc surface.

[0010] The lifting mechanism comprises a first motor fixed on the carrier plate, a second screw connected to the first motor, and a lifting plate connected to the second screw, and the ultrasonic generator is fixed on the lifting plate.

[0011] The outer circumference of the lifting plate is inwardly recessed to form a notch, and the ultrasonic generator is installed in the notch and locked by a locking member.

[0012] The locking member includes a locking half ring and a screw. Both ends of the locking half ring are provided with connecting ears. The connecting ears are provided with screw holes. The screws pass through the screw holes and are fastened to the lifting plate. The shape formed by the locking half ring and the recess is compatible with the outer peripheral shape of the ultrasonic generator.

[0013] The first translation mechanism includes a second motor, a first lead screw drivingly connected to the second motor, and a slider connected to the first lead screw, and the carrying plate is fixed to the upper end of the slider.

[0014] The fixed platform has a first through groove running through its upper and lower surfaces, the reagent kit includes at least one connecting tube, the fixing device includes a fixing seat provided with a second through groove, the second through groove runs through the upper and lower surfaces of the fixing seat, the first through groove is connected to the second through groove, the reagent kit is installed in the second through groove and the bottom of the reagent kit is aligned with the first through groove, and the vibration head passes through the first through groove and contacts with the bottom of the reagent kit.

[0015] The fixing device also includes a limit member, which is fixed to the fixing platform and is used to limit the fixing seat on the fixing platform. The limit member includes a first limit block and a second limit block arranged in parallel with the first limit block. The opposite inner surfaces of the first limit block and the second limit block are both provided with elastic members, and the fixing seat is correspondingly provided with a slot for clamping the elastic member.

[0016] In summary, due to the adoption of the above technical scheme, the beneficial effects of the present invention are: overcoming the defects of the existing non-direct contact ultrasonic nucleic acid extractor based on the oscillation module for lysis and mixing, such as long extraction time and high error rate due to heavy load, low oscillation frequency and reduced oscillation efficiency, and having a simple structure, small footprint, high stability, low noise, high mixing efficiency, good extraction effect and easy operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the non-direct contact ultrasonic nucleic acid extractor of the present invention;

[0018] Figure 2 for Figure 1 The main view of

[0019] Figure 3 for Figure 2 Schematic diagram of the ultrasonic component structure;

[0020] Figure 4 for Figure 3 A schematic diagram of the structure from another angle;

[0021] Figure 5 for Figure 4 The enlarged structural diagram at A in the middle;

[0022] Figure 6 for Figure 1 A schematic diagram of the structure from another angle;

[0023] Figure 7 for Figure 1 Schematic diagram of the assembly structure of the reagent kit and the fixing device;

[0024] Figure 8 for Figure 7 Schematic diagram of the decomposition structure;

[0025] Fig. 9 for Figure 8 Schematic diagram of the structure of the middle fixed seat;

[0026] Fig.10 for Figure 8 Schematic diagram of the central tube structure;

[0027] Fig.11 for Figure 8 Schematic diagram of the structure of the middle clamping piece;

[0028] Fig.12 for Figure 1 Schematic diagram of the structure of some components. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] See also Figures 1 to 12The present invention provides a non-direct contact ultrasonic nucleic acid extractor, which includes a base 1, an ultrasonic component 2 installed on the base 1 and used to vibrate and mix the nucleic acid solution to be extracted in a reagent box 5, a fixed platform 3 located above the ultrasonic component 2, a fixing device 4 fixed to the fixed platform 3 and used to fix the reagent box 2, a magnetic sleeve component 6, a magnetic rod component 7 that cooperates with the magnetic sleeve component 6, a first driving component 8 connected to the magnetic sleeve component 6, and a second driving component 9 connected to the magnetic rod component 7. The magnetic sleeve component 6 is located above the fixing device 4, and the magnetic rod component 7 is located above the magnetic sleeve component 6.

[0031] The base 1 is a plate-shaped structure, and the fixed platform 3 is fixed on the base 1 through the support plates 11 on both sides. The ultrasonic component 2 is installed above the base 1 and below the fixed platform 3. Figure 2 The ultrasonic component 2 includes a first translation mechanism 21 fixed to the surface of the base 1, a carrier plate 22 installed on the first translation mechanism 21, a lifting mechanism 23 fixed on the carrier plate 22, an ultrasonic generator 24 fixed on the lifting mechanism 23, and a vibration head 25 fixed to the output end of the ultrasonic generator 24.

[0032] See also Figure 3 The first translation mechanism 21 includes a second motor 211, a first lead screw 212 drivingly connected to the second motor 211, and a slider 213 connected to the first lead screw 212. The carrier plate 22 is fixed to the upper end of the slider 213. The second motor 211 drives the first lead screw 212 to rotate. The slider 213 on the first lead screw 212 drives the carrier plate 22 to translate, thereby realizing the horizontal movement of the ultrasonic generator 24.

[0033] Continue to see Figure 3 The lifting mechanism 23 includes a first motor 231 fixed on the carrier plate 22, a second screw rod 232 connected to the first motor 231, and a lifting plate 233 connected to the second screw rod 232. The ultrasonic generator 24 is fixed on the lifting plate 233. The first motor 231 controls the lifting plate 233 to move up and down through the second screw rod 232. The lifting plate 233 drives the ultrasonic generator 24 to move up and down as a whole, so as to achieve the contact or separation between the vibration head 25 of the ultrasonic generator 24 and the bottom of the reagent box 5.

[0034] The ultrasonic generator 24 is connected to a matching alternating current signal to drive the ultrasonic generator 24 to work. The electrical power of the ultrasonic generator 24 is converted into mechanical power (i.e., ultrasonic waves) and transmitted out. The vibration head 25 contacts the bottom of the reagent box 5 and is transmitted to the reagent box 5 through the vibration head 25. In other words, the nucleic acid solution to be extracted in the reagent box 5 is mixed, and there is no need to insert the vibration head 25 deeply into the reagent box 5. The vibration head 25 does not need to contact the nucleic acid solution to be extracted, that is, a non-direct contact mixing method is adopted, which can avoid cross infection. In addition, the ultrasonic generator 2 works at a predetermined mixing frequency, and the contact surface between the vibration head 25 and the reagent box 5 generates heat, thereby heating the nucleic acid solution in the reagent box 5 and eliminating the heating component.

[0035] See also Figure 4 In order to fix the ultrasonic generator 2, the outer circumference of the lifting plate 233 is concavely formed inwardly to form a notch 2331. The ultrasonic generator 2 is installed in the notch 2331 and is locked by a locking member 26. The locking member 26 includes a locking half ring 261 and a screw. Both ends of the locking half ring 26 are provided with connecting ears 2611. The connecting ears 2611 are provided with screw holes. The screws pass through the screw holes and are fastened to the lifting plate 233 to lock the ultrasonic generator 2 in the notch 2331. The shape formed by the locking half ring 261 and the notch 2331 is adapted to the outer circumferential shape of the ultrasonic generator 2.

[0036] See also Figure 4 and Figure 5 A plurality of profiling grooves 2521 are provided along the length direction of the outer end surface of the vibration head 25, and the plurality of profiling grooves 2521 are matched with the bottom of the reagent box 5. A narrow groove 2522 is also provided along the length direction of the outer end surface of the vibration head 25, and the narrow groove 2522 runs through the central area of ​​the plurality of profiling grooves 2521. The depth of the narrow groove 2522 is greater than the depth of the profiling groove 2521, so as to divide the profiling groove 2521 into two parts. Such a configuration is used to reduce mutual interference of ultrasound. If the narrow groove 2522 is not provided, the plurality of profiling grooves 2521 on the outer end surface of the vibration head 25 will cancel each other due to the interference of ultrasound, and the reagent solution cannot be vibrated.

[0037] In this embodiment, the narrow grooves 2522 can be set to have the same width, or the maximum width of the lower section of the narrow groove 2522 can be set to be greater than the width of the upper section of the narrow groove 2522. Figure 5 As shown, the lower section of the narrow groove 2522 forms a bottom groove 2523 , the maximum width of the bottom groove 2523 is greater than the width of the narrow groove 2522 , and the cross-section of the bottom groove 2523 is circular, rectangular, square, triangular, etc.

[0038] See also Figure 4The vibration head 25 includes a vibration head lower part 251 and a vibration head upper part 252 integrally formed with the vibration head lower part 251, and a plurality of profiling grooves 2521 are formed inwardly from the outer end surface of the vibration head upper part 252. The thickness of the vibration head lower part 251 is greater than that of the vibration head upper part 252, and the connection between the vibration head lower part 251 and the vibration head upper part 252 is transitioned through an arc surface 253.

[0039] The vibration head 25 is provided with at least one damping groove 254, which is used to reduce the lateral vibration along the horizontal axis of the vibration head 25. Specifically, in this embodiment, two damping grooves 254 are provided, and the two damping grooves 254 are symmetrically arranged about the central axis of the vibration head 25, which is conducive to reducing the lateral vibration and ensuring that the ultrasonic vibration is propagated along the longitudinal axis. Of course, in other embodiments, the number of the damping grooves 254 can also be one, three, four, etc.

[0040] See also Figure 6 The fixed platform 3 has a first through groove 31 that runs through the upper and lower surfaces thereof, see Figure 7 and Figure 8 The fixing device 4 is used to fix the reagent box 5. The reagent box 5 includes at least one connecting tube 51. One connecting tube 51 is used to hold a group of nucleic acid solutions to be extracted and perform a group of nucleic acid extraction. The fixing device 4 includes a fixing seat 41 provided with a second through groove 40, a moving part 42 and a pressing part 43. The second through groove 40 runs through the upper and lower surfaces of the fixing seat 41. The first through groove 31 is connected to the second through groove 40. The reagent box 5 is installed in the second through groove 40 and the bottom of the reagent box 5 is aligned with the first through groove 31. The vibration head 25 passes through the first through groove 31 and contacts the bottom of the reagent box 5. A plurality of groups of stoppers 416 are provided in the second through groove 40. Two adjacent groups of stoppers 416 form a limiting groove 417. A connecting tube 51 is placed in each limiting groove 417.

[0041] See also Fig. 9 The fixing seat 41 includes a first side 411, a second side 412 parallel to the first side 411, a third side 413 vertically connecting one end of the first side 411 and the second side 412, and a fourth side 414 vertically connecting the other end of the first side 411 and the second side 412. The second through groove 40 is further provided with a first inner side 415 connecting the third side 413 and the fourth side 414. The first inner side 415 divides the second through groove 40 into two fixed spaces 401. Both fixed spaces 401 can be used to fix multiple row pipes 51. A plurality of blocking parts 416 are provided in the two fixed spaces 401.

[0042] See also Figure 7 The fixing device 4 further includes a limiting member 44, which is fixed to the fixing platform 3 and is used to limit the fixing seat 41 to the fixing platform 3. Figure 8The limiting member 44 includes a first limiting block 441 and a second limiting block 442 spaced and arranged in parallel with the first limiting block 441. The inner surfaces of the first limiting block 441 and the second limiting block 442 facing each other are provided with an elastic member 4411. The fixing seat 41 is provided with a corresponding slot 418 for holding the elastic member 4411. By compressing the elastic member 4411, the slot 418 is aligned with the elastic member 4411, and the elastic member 4411 is fastened and fixed with the slot 418 of the fixing seat 41, thereby fixing the fixing seat 41. The elastic member 4411 can be a marble.

[0043] See also Figure 8 The moving part 42 is a rectangular frame structure, and a plurality of row tubes 51 can be placed side by side in the frame. The frame includes a first frame 421, a second frame 422, a third frame 423, and a fourth frame 424. A second inner edge 425 is connected between the first frame 421 and the third frame 423. The second inner edge 425 divides the frame into two placement spaces 426. Multiple row tubes 51 can be placed in both placement spaces 426. When nucleic acid extraction is required, a preset number of row tubes 51 are placed in the placement space 426.

[0044] See also Fig.10 The connecting tubes 51 each have a plurality of liquid storage tanks 501. A first holding block 511 is provided at one end of the connecting tube 51, and a second holding block 512 is provided at the other end. The first holding block 511 and the second holding block 512 are used to hold the moving member 42. The liquid storage tank 501 at one end of the connecting tube 51 is expanded outward to form an expansion tank 502. The bottom of the expansion tank 502 and one end of the connecting tube 51 form a recess 503. The first holding block 511 is provided at the bottom of the expansion tank 502 and extends toward the recess 503. The second holding block 512 is provided at the other end of the connecting tube 51.

[0045] A plurality of clamping strips 513 are disposed on both sides of the connecting pipe 51. The movable member 42 installs the connecting pipe 51 in the limiting groove 417 of the fixing seat 41, and the clamping strips 513 of the connecting pipe 51 abut against the upper surface of the fixing seat 41.

[0046] See also Fig.11 The clamping piece 43 has a third through groove 431 running through its upper and lower surfaces, and the inner wall of the clamping piece 43 is provided with a side groove 432 recessed inwardly. The clamping piece 43 is sleeved on the outside of the connecting pipe 51 installed on the fixing seat 41. A connecting column 433 is provided at the bottom of the clamping piece 43, and a connecting groove 419 is provided on the fixing seat 41. The connecting column 433 is positioned and assembled with the connecting groove 419.

[0047] See also Fig.12The first driving assembly 8 and the second driving assembly 9 are respectively fixed on a driving mounting plate 10, which are used to drive the magnetic sleeve assembly 6 and the magnetic rod assembly 7 respectively. The first driving assembly 8 includes a third motor 81 fixed on the driving mounting plate 10, a first pulley assembly 82 fixed on the driving mounting plate 10, and a first connecting block 83 fixed on the first pulley assembly 82, and the magnetic sleeve assembly 6 is fixed on the first connecting block 83.

[0048] The second driving assembly 9 includes a fourth motor 91 fixed on the driving mounting plate 10 , a second pulley assembly 92 fixed on the driving mounting plate 10 , and a second connecting block 93 fixed on the second pulley assembly 92 , and the magnetic bar assembly 7 is fixed to the second connecting block 93 .

[0049] The third motor 81 rotates to drive the belt in the first pulley assembly 82 to rotate, thereby driving the first connecting block 83 to move in the vertical direction, thereby driving the magnetic sleeve assembly 6 to move up and down. The fourth motor 91 rotates to drive the belt in the second pulley assembly 92 to rotate, thereby driving the second connecting block 93 to move in the vertical direction, thereby driving the magnetic bar assembly 7 to move up and down.

[0050] The drive mounting plate 10 is vertically arranged and mounted on the second translation mechanism 101. The second translation mechanism 101 drives the first drive assembly 8 and the second drive assembly 9 to move horizontally, thereby driving the magnetic sleeve assembly 6 and the magnetic rod assembly 7 to translate as a whole at the same time. The translation distance is determined according to the volume position in the reagent box 5.

[0051] The second translation mechanism 101 is driven by a motor to drive the screw rod to rotate, and the slider on the screw rod drives the carrier plate mounting plate 10 to translate.

[0052] See also Fig.12 The magnetic sleeve assembly 6 includes a magnetic sleeve fixing plate 61 fixed to the first connecting block 83, a pair of magnetic sleeve fixing blocks 62 fixed below the magnetic sleeve fixing plate 61, a magnetic sleeve holding plate 63 clamped in the magnetic sleeve fixing block 62, and a plurality of magnetic sleeves 64 fixedly mounted on the magnetic sleeve holding plate 63. The magnetic sleeve fixing block 62 has a holding groove (not shown) inside, and the magnetic sleeve holding plate 63 is inserted into the holding groove from the end of the magnetic sleeve fixing block 62. The magnetic sleeve 64 is a hollow tubular structure.

[0053] Continue to see Fig.12 The magnetic bar assembly 7 includes a magnetic bar fixing plate 71 fixed to the second connecting block 93, a pair of magnetic bar fixing blocks 72 fixed below the magnetic bar fixing plate 71, a magnetic bar holding plate 73 clamped in the magnetic bar fixing block 72, and a plurality of magnetic bars 74 fixedly mounted on the magnetic bar holding plate 73. The magnetic bar fixing block 72 has a holding groove (not shown) inside, and the magnetic bar holding plate 73 is inserted into the holding groove from the end of the magnetic bar fixing block 72. The magnetic bar 74 has a rod-shaped structure, and its lowermost end is a magnetic metal.

[0054] During operation, the reagent box 5 is placed on the fixing device 4, the first container of the reagent box 5 contains the nucleic acid to be extracted, the fixing device 4 fixes the reagent box 5, and the auxiliary solution is added to the reagent box 5. The first translation mechanism 21 is driven to align the vibration head 25 above the ultrasonic generator 24 with the bottom of the first container of the reagent box 5, and the ultrasonic generator 24 and the vibration head 25 are driven upward by the lifting mechanism 23 to move upward, and the vibration head 25 passes through the first through groove 31 and contacts the bottom of the reagent box 5. By driving the ultrasonic generator 24, the solution in the first container is evenly mixed. The ultrasonic generator 24 continuously emits ultrasonic waves to the first container through the vibration head 25, and the solution is vibrated and evenly mixed. Then, through the second translation mechanism 101, the magnetic sleeve 64 and the magnetic rod 74 are aligned with the first container slot of the reagent box 5, and then the third motor 81 and the fourth motor 91 are started successively to make the magnetic sleeve 64 and the magnetic rod 74 descend, and the magnetic sleeve 64 descends to the specified position. The fourth motor 91 drives the magnetic rod 74 to pass through the magnetic sleeve 64 and continue to contact the mixed solution downward, and then lift the magnetic sleeve 64 and the magnetic rod 74, and then the magnetic rod 74 is placed in the container slot at the rear of the reagent box 5 step by step to elute and extract the nucleic acid. The magnetic sleeve 64 does not contact the solution, and the magnetic sleeve 64 moves with the magnetic rod 74 to protect the solution in the magnetic rod 74 from external contamination. It is necessary that the first translation mechanism 21 drives the ultrasonic generator and the vibration head 25 to translate to the bottom of a container slot of the next reagent box that needs to be shaken and mixed, and mix the solution again.

[0055] The ultrasonic generator 24 and its vibration head 25 continuously emit ultrasonic waves to the reagent box 5, and it only takes 3-5 minutes to complete the mixing of a reagent box solution. The present invention can overcome the defects of the existing non-direct contact ultrasonic nucleic acid extractor based on the oscillation module for lysis and mixing, such as long extraction time and high error rate due to heavy load, low oscillation frequency and reduced oscillation efficiency, and has a simple structure, small footprint, high stability, low noise, high oscillation efficiency, good extraction effect and easy operation.

[0056] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A non-direct contact ultrasonic nucleic acid extractor, characterized in that: It comprises a base, an ultrasonic component mounted on the base, a fixed platform located above the ultrasonic component, and a fixing device fixed on the fixed platform and used to fix the reagent box, wherein the ultrasonic component comprises a first translation mechanism fixed on the surface of the base, a carrier plate mounted on the first translation mechanism, a lifting mechanism fixed on the carrier plate, an ultrasonic generator fixed on the lifting mechanism, and a vibration head fixed on the output end of the ultrasonic generator, a plurality of profiling grooves are arranged along the length direction of the outer end surface of the vibration head, and the plurality of profiling grooves are matched with the bottom of the reagent box, and a narrow groove is also arranged along the length direction of the outer end surface of the vibration head, the narrow groove runs through the central area of ​​the plurality of profiling grooves, and the depth of the narrow groove is greater than the depth of the profiling groove; the lower section of the narrow groove forms a bottom groove, the maximum width of the bottom groove is greater than the width of the upper section of the narrow groove, and the cross section of the bottom groove adopts a circle, a rectangle, a square, a trapezoid, or a triangle; The fixed platform has a first through groove running through its upper and lower surfaces, the reagent kit includes at least one connecting tube, the fixing device includes a fixing seat provided with a second through groove, the second through groove runs through the upper and lower surfaces of the fixing seat, the first through groove is connected to the second through groove, the reagent kit is installed in the second through groove and the bottom of the reagent kit is aligned with the first through groove, and the vibration head passes through the first through groove and contacts with the bottom of the reagent kit.

2. The non-direct contact ultrasonic nucleic acid extractor according to claim 1, characterized in that: The vibration head comprises a vibration head lower part and a vibration head upper part integrally formed with the vibration head lower part, and a plurality of contoured grooves are formed inwardly from the outer end surface of the vibration head upper part.

3. The non-direct contact ultrasonic nucleic acid extractor according to claim 2, characterized in that: The thickness of the lower part of the vibration head is greater than the thickness of the upper part of the vibration head, and the connection between the lower part of the vibration head and the upper part of the vibration head is transitioned through an arc surface.

4. The non-direct contact ultrasonic nucleic acid extractor according to claim 3, characterized in that: The lifting mechanism comprises a first motor fixed on the carrier plate, a second screw connected to the first motor, and a lifting plate connected to the second screw, and the ultrasonic generator is fixed on the lifting plate.

5. The non-direct contact ultrasonic nucleic acid extractor according to claim 4, characterized in that: The outer circumference of the lifting plate is inwardly recessed to form a notch, and the ultrasonic generator is installed in the notch and locked by a locking member.

6. The non-direct contact ultrasonic nucleic acid extractor according to claim 5, characterized in that: The locking member includes a locking half ring and a screw. Both ends of the locking half ring are provided with connecting ears. The connecting ears are provided with screw holes. The screws pass through the screw holes and are fastened to the lifting plate. The shape formed by the locking half ring and the recess is compatible with the outer peripheral shape of the ultrasonic generator.

7. The non-direct contact ultrasonic nucleic acid extractor according to claim 6, characterized in that: The first translation mechanism includes a second motor, a first lead screw drivingly connected to the second motor, and a slider connected to the first lead screw, and the carrying plate is fixed to the upper end of the slider.

8. The non-direct contact ultrasonic nucleic acid extractor according to claim 1, characterized in that: The fixing device also includes a limit member, which is fixed to the fixing platform and is used to limit the fixing seat on the fixing platform. The limit member includes a first limit block and a second limit block arranged in parallel with the first limit block. The opposite inner surfaces of the first limit block and the second limit block are both provided with elastic members, and the fixing seat is correspondingly provided with a slot for clamping the elastic member.

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