A fiber optic device and method for real-time detection and analysis of zebrafish heart rate

Abstract

This invention discloses an optical fiber device and method for real-time detection and analysis of zebrafish heart rate. The device includes a mounting slot, an optical signal transmitter, and an optical signal receiver / processor. The mounting slot is used to place and fix the zebrafish. The top of the mounting slot has a detection signal input port, and the bottom has a detection signal output port corresponding to the detection signal input port. The optical signal transmitter is connected to the detection signal input port, and the optical signal receiver / processor is connected to the detection signal output port. A light source is introduced through an optical fiber to irradiate the heart region of the fixed zebrafish. The irradiated light signal is received by a spectrometer, and then the signal output by the spectrometer is used to perform algorithmic calculations to determine the zebrafish's heart rate. This invention utilizes optical signals for detection, which significantly reduces errors caused by manual operation compared to conventional microscopic methods, and can significantly improve the accuracy of heart rate measurement.

Description

Technical Field

[0001] This invention relates to the field of fiber optic sensing technology, and more specifically to a fiber optic device and method for real-time detection and analysis of zebrafish heart rate. Background Technology

[0002] Zebrafish are a common tropical fish. Their genes share 87% similarity with human genes, meaning that drug experimental results obtained on zebrafish are, in most cases, applicable to humans. Furthermore, zebrafish are highly prolific, with a short breeding cycle and high egg production; fertilized eggs hatch into fry in just 2-3 days. In addition, zebrafish are small and easy to raise, and their transparent embryos make it easy to observe the effects of drugs on their internal organs. Therefore, zebrafish can serve as a model for human diseases, used to assess the effects and efficacy of drugs in human treatments.

[0003] Heart disease is a complex condition, and heart rate is the most direct reflection of normal heart function. For high-risk individuals, long-term, continuous heart rate monitoring can establish the first line of defense. Continuous and accurate real-time monitoring and analysis of heart rate can promptly detect abnormalities in cardiac activity, giving patients valuable time for diagnosis and intervention. Due to the high genetic similarity between zebrafish and humans, monitoring zebrafish heart rate changes can provide insights for monitoring human heart health. Real-time measurement of zebrafish heart rate allows for precise calculation of highly useful health information, enabling the prediction of human heart disease and early, targeted treatment and prevention. Therefore, real-time measurement of zebrafish heart rate is of great significance for research on the prevention and treatment of human heart disease.

[0004] Currently, many researchers have conducted research on zebrafish heart rate. For example, "Study on the Effects of Arecoline Hydrobromide on Zebrafish Embryonic Development and Cardiotoxicity" co-authored by Jia Zhe, Dong Haipeng, et al. was published in Global Chinese Medicine, 2021.14(07); "Study on the Toxicity Effects of Bisphenol AP on Zebrafish Heart Development" co-authored by Wang Hongye, Cao Cheng, et al. was published in Environmental Science and Technology, 2019.42(08); "Effects of Isoglycyrrhizin on Zebrafish Embryonic Development, Angiogenesis and Heart" co-authored by He Junlin, Yu Si, et al. was published in Sichuan Zoology, 2018.37(06); "Effects of Isoglycyrrhizin on Zebrafish Embryonic Development, Angiogenesis and Heart" co-authored by Wang Xiaoya, Miao Lulin, et al. was published in Chinese Journal of Traditional Chinese Medicine, 2021.39(01); and "Methods for Identifying and Monitoring the Heart of Zebrafish Juveniles under Micromanipulation" co-authored by Tong Mingsi, Huang Gang, et al. was published in Journal of Harbin Institute of Technology, 2020.52(06). These researchers all used microscopic observation to monitor the heart rate of zebrafish. However, since the diameter of a zebrafish heart is generally about 100 μm, microscopic observation is difficult to operate and observe, and is easily affected by adverse factors such as human operation and environmental noise.

[0005] Therefore, how to provide a simple and effective fiber optic device and method for real-time detection and analysis of zebrafish heart rate is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides an optical fiber device and method for real-time detection and analysis of zebrafish heart rate, aiming to solve the above-mentioned technical solutions.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A fiber optic device for real-time detection and analysis of zebrafish heart rate, comprising:

[0009] A fixing slot; the fixing slot is used to place and fix the zebrafish; the top of the fixing slot has a detection signal input port, and the bottom has a detection signal output port corresponding to the detection signal input port;

[0010] Optical signal transmitter; the optical signal transmitter is connected to the detection signal input port;

[0011] Optical signal receiving and processing end; the optical signal receiving and processing end is connected to the detection signal output port, and performs algorithm processing on the received optical signal to obtain the heart rate of the zebrafish.

[0012] Through the above technical solution, the optical fiber device provided by the present invention uses a method of fixing the zebrafish, and uses an optical signal transmitting end and an optical signal receiving and processing end to process the optical signal of the zebrafish, which can ensure that the zebrafish's heart rate changes can be monitored in real time without damage.

[0013] Preferably, in the aforementioned fiber optic device for real-time detection and analysis of zebrafish heart rate, the fixing slot is encased in a metal shell. By using a metal enclosure, interference from adverse factors such as environmental noise is effectively avoided or reduced; the fixing slot is a cuboid slightly larger than the zebrafish.

[0014] Preferably, in the above-mentioned fiber optic device for real-time detection and analysis of zebrafish heart rate, the optical signal transmitting end includes a light source and a first fiber bundle connected to the transmitting end of the light source; the end of the first fiber bundle away from the light source is connected to the detection signal input port. This satisfies the optical signal output of the light source.

[0015] Preferably, in the aforementioned fiber optic device for real-time detection and analysis of zebrafish heart rate, the optical signal receiving and processing end includes a second fiber bundle connected to the detection signal output port, a spectrometer connected to the end of the second fiber bundle away from the detection signal output port, and a signal acquisition module, an A / D conversion module, a microcontroller, and an LCD screen sequentially electrically connected to the output port of the spectrometer. The microcontroller performs algorithmic processing on the feedback signal and transmits it to the LCD screen to display the zebrafish's heart rate graph.

[0016] Preferably, in the above-mentioned fiber optic device for real-time detection and analysis of zebrafish heart rate, the optical signal receiving and processing end further includes a temperature sensor installed inside the fixed slot; the temperature sensor is electrically connected to the signal acquisition module through a signal line and is capable of detecting the temperature inside the fixed slot.

[0017] Preferably, in the aforementioned fiber optic device for real-time detection and analysis of zebrafish heart rate, the zebrafish's heart, fixed in the fixing slot, is within the range of the light spot introduced through the detection signal input port, while the zebrafish's eyes are outside the range of the light spot introduced through the detection signal input port. This allows for the detection of the zebrafish's heart rate while preventing damage to its eyes.

[0018] The present invention also provides a method for real-time detection and analysis of zebrafish heart rate. The method involves irradiating a fixed zebrafish heart area with a light source through an optical fiber, receiving the irradiated light signal through a spectrometer, and then performing algorithm calculations on the signal output by the spectrometer to obtain the zebrafish heart rate.

[0019] Through the above technical solution, the method provided by the present invention does not require the implantation of electrodes and uses light signals for detection. Compared with the usual microscopic method, it greatly reduces the error caused by manual operation and can significantly enhance the accuracy of heart rate measurement.

[0020] Preferably, in the above-described method for real-time detection and analysis of zebrafish heart rate, the algorithm employs either a frame brightness algorithm or a variable pixel intensity algorithm. The frame brightness algorithm tracks the change in average light intensity of each frame to calculate the heart rate; alternatively, the variable pixel intensity algorithm detects the heart rate by comparing the intensity changes of pixels from one frame to the next.

[0021] Preferably, in the above-described method for real-time detection and analysis of zebrafish heart rate, the zebrafish is an unlabeled zebrafish, or a zebrafish labeled with a luminescent or fluorescent group; the spectral signal tested by the spectrometer includes the signal caused by the heartbeat of an unlabeled zebrafish, or the luminescent or fluorescent signal caused by the heartbeat of a zebrafish labeled with a luminescent or fluorescent group. This enhances the applicability of the detection.

[0022] Preferably, in the above-mentioned method for real-time detection and analysis of zebrafish heart rate, the zebrafish used for detection is in a fixed state or a sleep state, avoiding the influence of factors such as zebrafish swimming around and water flow, and can more accurately measure the actual heart rate of the zebrafish.

[0023] As can be seen from the above technical solution, compared with the prior art, the present invention discloses an optical fiber device and method for real-time detection and analysis of zebrafish heart rate, which has the following beneficial effects:

[0024] 1. This invention does not require the implantation of electrodes and can ensure that the heart rate changes of zebrafish can be monitored in real time without damage.

[0025] 2. By using a metal enclosed box, the present invention effectively avoids or reduces the interference of adverse factors such as environmental noise.

[0026] 3. The present invention uses a method of fixing the zebrafish in place, which avoids the influence of factors such as the zebrafish swimming around and water flow, and can more accurately measure the actual heart rate of the zebrafish.

[0027] 4. Compared with conventional microscope methods, this invention greatly reduces the errors caused by manual operation and can significantly enhance the accuracy of heart rate measurement.

[0028] 5. This invention provides a certain reference for studying the impact of changes in the external environment on the human heart and for judging heart rate diseases, and also provides a powerful assessment tool for the development of human heart drugs.

[0029] 6. This invention can accurately identify and provide heartbeat and heart rate curves, and has the advantages of real-time monitoring, speed, and ease of operation. It is expected to play an important role in the prevention and treatment of human heart and heart rate diseases and has broad commercial application prospects. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0031] Figure 1 The attached figure is a schematic diagram of the fiber optic device for real-time detection and analysis of zebrafish heart rate provided by the present invention.

[0032] in:

[0033] 1 is the light source; 2 is the first fiber optic bundle; 3 is the fixing slot; 4 is the metal shell; 5 is the zebrafish; 6 is the temperature sensor; 7 is the second fiber optic bundle; 8 is the spectrometer; 9 is the signal acquisition module; 10 is the A / D conversion module; 11 is the microcontroller; 12 is the display screen; 13 is the detection signal input port; 14 is the detection signal output port. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] See appendix Figure 1 This invention discloses an optical fiber device for real-time detection and analysis of zebrafish heart rate, comprising:

[0036] Fixing groove 3; fixing groove 3 is used to place and fix zebrafish 5; the top of fixing groove 3 has a detection signal input port 13, and the bottom has a detection signal output port 14 corresponding to the detection signal input port 13;

[0037] Optical signal transmitter; the optical signal transmitter is connected to the detection signal input port 13;

[0038] Optical signal receiving and processing end; the optical signal receiving and processing end is connected to the detection signal output port 14, and performs algorithm processing on the received optical signal to obtain the heart rate of zebrafish 5.

[0039] To further optimize the above technical solution, the outside of the fixing groove 3 is wrapped with a metal shell 4.

[0040] To further optimize the above technical solution, the optical signal transmitting end includes a light source 1 and a first optical fiber bundle 2 connected to the transmitting end of the light source 1; the end of the first optical fiber bundle 2 away from the light source 1 is connected to the detection signal input port 13.

[0041] To further optimize the above technical solution, the optical signal receiving and processing end includes a second optical fiber bundle 7 connected to the detection signal output port 14, a spectrometer 8 connected to the end of the second optical fiber bundle 7 away from the detection signal output port 14, and a signal acquisition module 9, an A / D conversion module 10, a microcontroller 11 and an LCD screen 12 that are electrically connected in sequence to the output port of the spectrometer 8.

[0042] To further optimize the above technical solution, the optical signal receiving and processing end also includes a temperature sensor 6 installed inside the fixed slot 3; the temperature sensor 6 is electrically connected to the signal acquisition module 9 through a signal line.

[0043] To further optimize the above technical solution, the heart of the zebrafish 5 fixed in the fixing groove 3 is within the range of the light spot introduced by the detection signal input port 13, while the eyes of the zebrafish 5 are outside the range of the light spot introduced by the detection signal input port 13.

[0044] The method for real-time detection and analysis of zebrafish heart rate using the fiber optic device described above is as follows:

[0045] The light source 1 is introduced through the first optical fiber bundle 2 to irradiate the heart area of ​​the zebrafish 5 fixed in the fixing slot 3. The irradiated light signal is received and exported through the second optical fiber bundle 7 and received by the spectrometer 8. The output port of the spectrometer 8 is connected to the signal acquisition module 9, which is then connected to the A / D conversion module 10. The A / D conversion module 10 is connected to the microcontroller 11. The microcontroller 11 processes the feedback signal using an algorithm, employing either a frame brightness algorithm or a variable pixel intensity algorithm, to calculate the heart rate of the zebrafish 5 and transmit it to the LCD screen 12 to display the heart rate graph of the zebrafish 5.

[0046] The zebrafish are either unlabeled zebrafish or zebrafish labeled with luminescent or fluorescent groups; the spectral signals measured by the spectrometer include signals caused by the heartbeat of unlabeled zebrafish, or luminescent or fluorescent signals caused by the heartbeat of zebrafish labeled with luminescent or fluorescent groups.

[0047] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to in the method section.

[0048] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A fiber optic device for real-time detection and analysis of zebrafish heart rate, characterized by, include: Fixing groove (3); the fixing groove (3) is used to place and fix zebrafish (5); the top of the fixing groove (3) has a detection signal input port (13), and the bottom has a detection signal output port (14) corresponding to the detection signal input port (13); Optical signal transmitter; the optical signal transmitter is connected to the detection signal input port (13); Optical signal receiving and processing end; The optical signal receiving and processing terminal is connected to the detection signal output port (14) and performs algorithm processing on the received optical signal to obtain the heart rate of the zebrafish (5). The light source is introduced through an optical fiber to irradiate the heart area of ​​a fixed zebrafish, and the light signal after irradiation is received by a spectrometer. The signal output by the spectrometer is then calculated by an algorithm to obtain the heart rate of the zebrafish. The algorithm employs either a frame brightness algorithm or a variable pixel intensity algorithm. The zebrafish are unlabeled zebrafish or zebrafish labeled with luminescent or fluorescent groups; the spectral signals tested by the spectrometer include signals caused by the heartbeat of unlabeled zebrafish, or luminescent or fluorescent signals caused by the heartbeat of zebrafish labeled with luminescent or fluorescent groups. The zebrafish used for testing were either stationary or asleep. The optical signal receiving and processing terminal also includes a temperature sensor (6) installed inside the fixed slot (3); the temperature sensor (6) is electrically connected to the signal acquisition module (9) via a signal line; The heart of the zebrafish (5) fixed in the fixing groove (3) is within the range of the light spot introduced by the detection signal input port (13), while the eyes of the zebrafish (5) are outside the range of the light spot introduced by the detection signal input port (13). The fixing groove (3) is wrapped with a metal shell (4) on the outside; The optical signal transmitting end includes a light source (1) and a first optical fiber bundle (2) connected to the transmitting end of the light source (1); the end of the first optical fiber bundle (2) away from the light source (1) is connected to the detection signal input port (13); The optical signal receiving and processing end includes a second optical fiber bundle (7) connected to the detection signal output port (14), a spectrometer (8) connected to the end of the second optical fiber bundle (7) away from the detection signal output port (14), and a signal acquisition module (9), an A / D conversion module (10), a microcontroller (11), and a liquid crystal display (12) that are electrically connected in sequence to the output port of the spectrometer (8).

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