An antiviral and antibacterial Chinese medicine composition containing Rhizoma Cynomorii and its preparation method and application

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and in particular to an antiviral and antibacterial Chinese medicine composition containing Rhizoma Cyrthospermi and its preparation method and application. The Chinese medicine composition comprises the following raw materials: honeysuckle, isatis root, Rhizoma Cyrthospermi, patchouli, rhizoma anemarrhenae, ephedra, arctium, peucedanum and rhodiola rosea. The antiviral composition can quickly relieve and effectively cure the symptoms of sore throat, fever, aversion to cold, thirst, dry mouth, chest tightness, white tongue coating, bright red tongue and rapid pulse, and has no adverse reactions and side effects. Animal experiments and antibacterial experiments have proved that the antibacterial and antiviral Chinese medicine composition has significant antiviral, antibacterial and antipyretic effects, so it can be prepared into medicines for treating the above symptoms and antiviral and antibacterial drugs.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to an antiviral and antibacterial Chinese medicine composition containing Cyperus rotundus and a preparation method and application thereof. Background Art

[0002] In the hot summer, most people often stay in air-conditioned rooms for a long time, which greatly reduces their activity level and consumes more cold food. Over time, this will cause a certain degree of damage to the body. In addition, the days are long and the nights are short in summer, and people are more active at night than in other seasons, which can easily lead to lack of sleep and disordered work and rest. Therefore, many diseases occur frequently in summer. Pathogenic factors such as internal heat, wind evil, summer heat, cold and dampness, and viruses in the stale air in air-conditioned rooms all have opportunities to cause external diseases such as wind-heat cold, wind-cold cold, viral infection, and internal diseases such as "getting angry". Sore throat is a common symptom in summer. Due to different pathogenic factors, it may be accompanied by other symptoms such as fever, aversion to cold, chest tightness, etc.

[0003] Western medicine can only detect some abnormal conditions in the body during the diagnosis of the above diseases, so Western medicine can only be selected according to the abnormal conditions, such as using anti-inflammatory drugs and antibiotics to treat sore throats, and using antipyretics to lower body temperature. Therefore, the therapeutic effect is not good, and new discomfort may be caused by side effects and adverse reactions. Summary of the invention

[0004] In view of the technical problems that current western medicines are poorly effective in treating sore throats in summer and other associated symptoms and may have side effects and adverse reactions, the present invention provides an antiviral and antibacterial Chinese medicine composition containing Rhizoma Cynomorii and a preparation method and application thereof.

[0005] In order to achieve the above-mentioned object of the invention, the embodiment of the present invention adopts the following technical solution:

[0006] On the one hand, an embodiment of the present invention provides an antibacterial and antiviral Chinese medicine composition, comprising the following raw materials in proportions by weight: 7-12 parts of honeysuckle, 11-18 parts of isatis root, 4-8 parts of cyrtomium wilfordii, 7-12 parts of patchouli, 6-11 parts of anemarrhena, 6-11 parts of ephedra, 8-13 parts of burdock seeds, 4-8 parts of peucedanum and 2-4 parts of rhodiola rosea.

[0007] In the antibacterial and antiviral Chinese medicine composition provided by the present invention:

[0008] Honeysuckle is cold in nature and enters the lung, heart, stomach, and large intestine meridians. It is good at dispersing wind-heat in the lung meridian, clearing away heat and toxins from the heart and lungs. It has a light and dispersing nature, and has a strong ability to clear away heat and detoxify, reduce swelling and disperse nodules, and can also cool blood and stop diarrhea.

[0009] Isatis root is cold in nature and enters the heart meridian and stomach meridian. It is good at clearing away heat and fire toxins, cooling blood and relieving sore throat, reducing swelling and dispersing nodules.

[0010] Herba Corydalis is slightly cold in nature, enters the liver and stomach meridians, clears away heat and toxic substances, expels parasites and stops bleeding.

[0011] Patchouli is slightly warm in nature and enters the spleen, stomach, and lung meridians. It can not only transform dampness and turbidity in the middle-jiao spleen and stomach, and harmonize the stomach and stop vomiting, but it can also transform dampness and relieve exterior symptoms, dissipate heat and detoxify, and it is warm in nature without being dry.

[0012] Anemarrhena asphodeloides is cold in nature and enters the lung, stomach, and kidney meridians. It can not only clear away heat and reduce fire, but also nourish yin, produce body fluid and moisten the dry lungs.

[0013] Ephedra is warm in nature and enters the lung and bladder meridians. It is light in quality and floats upward. It can promote lung qi, open the pores, dispel wind and cold, induce sweat and relieve exterior symptoms. It also has the effect of promoting diuresis and reducing swelling.

[0014] Burdock seed is cold in nature and enters the lung and stomach meridians. It can dispel wind-heat, relieve heat and detoxify, clear the lungs, promote rashes, relieve sore throats, disperse lumps and reduce swelling, and also facilitate bowel movements.

[0015] Peucedanum is slightly cold in nature and enters the lung meridian. It can dispel wind-heat, lower qi, clear the lungs and eliminate phlegm.

[0016] Rhodiola rosea is mild in nature and enters the lung and heart meridians. It has the functions of replenishing qi and clearing the lungs, invigorating qi and activating blood circulation, unblocking meridians and relieving asthma, dispersing blood stasis and reducing swelling.

[0017] Isatis root clears away heat and detoxifies, cools blood and relieves sore throat; burdock seeds are pungent and warm, enter the twelve meridians, treat wind-heat in the throat, disperse qi and eliminate phlegm, so they are especially good at treating sore throat and dry cough. The two medicines are the main ones for clearing away heat. In the formula, honeysuckle, ephedra, and peucedanum are the ministers for dispelling internal heat. Burdock seeds, combined with Corydalis cyrtonema and Rhizoma Anemarrhenae, can clear phlegm and heat in the lungs and strengthen the effect of clearing sore throat. In order to prevent the coldness of many medicines from being blocked inside, patchouli is added to expand the chest and regulate qi. The earth can produce fluid and carry the medicine upward, which is the guiding medicine, and rhodiola rosea can replenish the positive qi. The two are the assistants.

[0018] Clinical application results show that the whole prescription, through the combination of the above medicinal flavors in specific dosage, can quickly relieve and effectively cure the symptoms of sore throat, fever, aversion to cold, thirst, dry mouth, chest tightness, white tongue coating, bright red tongue, and rapid pulse, without adverse reactions and side effects. Animal tests and antibacterial tests have proved that the antibacterial and antiviral Chinese medicine composition has significant antiviral, antibacterial and antipyretic effects.

[0019] Preferably, the antibacterial and antiviral Chinese medicine composition comprises the following raw materials in proportions by weight: 8-10 parts of honeysuckle, 13-17 parts of isatis root, 5-7 parts of cyrtomium wilfordii, 8-10 parts of patchouli, 8-10 parts of anemarrhena asphodeloides, 8-10 parts of ephedra, 9-12 parts of burdock seeds, 5-7 parts of peucedanum and 3-4 parts of rhodiola rosea.

[0020] Preferably, the antibacterial and antiviral Chinese medicine composition comprises the following raw materials in proportion by weight: 9 parts of honeysuckle, 15 parts of isatis root, 6 parts of cyrtomium wilfordii, 9 parts of patchouli, 9 parts of anemarrhena asphodeloides, 9 parts of ephedra, 10 parts of burdock seeds, 6 parts of peucedanum and 3 parts of rhodiola rosea.

[0021] In actual use of this prescription, each weight portion above is 1g, which is the dosage per dose. Use 1 to 2 doses per day depending on the condition of the disease.

[0022] In the second aspect, the embodiment of the present invention also provides a method for preparing the above-mentioned antibacterial and antiviral Chinese medicine composition, comprising the following operations: weighing each raw material according to the raw material ratio in the antibacterial and antiviral Chinese medicine composition, extracting the volatile oil of the patchouli, filtering the residue and the honeysuckle, isatis root, cyperus rotundus, rhizoma anemarrhenae, ephedra, arctium, peucedanum and rhodiola rosea after decoction extraction, collecting the obtained filtrate, drying, and obtaining a decoction extract; adding the volatile oil of the patchouli to the obtained decoction extract and mixing evenly to obtain the antibacterial and antiviral Chinese medicine composition. The product prepared by the preparation method can be further processed into oral dosage forms such as tablets, capsules, granules, powders, syrups, suspensions, large honey pills, and dripping pills according to the actual needs of clinical use or dosage form requirements.

[0023] The decoction extract can be prepared by the following operation process: weigh each raw material according to the raw material ratio in the above antibacterial and antiviral Chinese medicine composition, decoct the residue after extracting volatile oil from patchouli and the honeysuckle, isatis root, cyperus rotundus, rhizoma anemarrhenae, ephedra, arctium, peucedanum and rhodiola rosea in an amount equivalent to 6 to 10 times the total weight of the honeysuckle, isatis root, cyperus rotundus, rhizoma anemarrhenae, ephedra, arctium, peucedanum and rhodiola rosea for 2 to 3 times, each time for 25 to 40 minutes, filter to obtain a decoction filtrate, dry the decoction filtrate to obtain a decoction extract. Preferably, decoct twice, each time for 35 minutes.

[0024] The drying method in the preparation method can be spray drying, reduced pressure drying or freeze drying. Before drying, reduced pressure concentration can be performed to shorten the drying time.

[0025] In a third aspect, an embodiment of the present invention further provides the use of the above-mentioned antibacterial and antiviral Chinese medicine composition in the preparation of a medicine for treating sore throat.

[0026] In a fourth aspect, an embodiment of the present invention further provides the use of the above-mentioned antibacterial and antiviral Chinese medicine composition in the preparation of antipyretic drugs.

[0027] In a fifth aspect, an embodiment of the present invention further provides the use of the above-mentioned antibacterial and antiviral Chinese medicine composition in the preparation of a medicine for treating sore throat combined with fever or chills.

[0028] In a sixth aspect, an embodiment of the present invention further provides the use of the above-mentioned antibacterial and antiviral Chinese medicine composition in the preparation of a medicine for treating sore throat combined with fever and chills.

[0029] In a seventh aspect, an embodiment of the present invention further provides the use of the above-mentioned antibacterial and antiviral Chinese medicine composition in the preparation of a drug for treating at least one symptom of sore throat, fever, aversion to cold combined with dry mouth, and chest tightness.

[0030] Clinically verified, the above antibacterial and antiviral Chinese medicine composition has good improvement and therapeutic effects on the symptoms of sore throat, fever, chills, thirst, dry mouth, chest tightness, white tongue coating, bright red tongue, and rapid pulse, so it can be used to prepare a medicine for treating one or more of the above symptoms.

[0031] In an eighth aspect, an embodiment of the present invention further provides an antiviral and / or antibacterial drug, which includes the above-mentioned antibacterial and antiviral Chinese medicine composition. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. The experimental methods used in the following examples are conventional methods in the art unless otherwise specified.

[0033] The honeysuckle, isatis root, cyperus rotundus, patchouli, anemarrhena, ephedra, arctium, peucedanum and rhodiola rosea used in the following examples all comply with the relevant provisions under the various medicinal materials in the main text of Volume 1 of the Chinese Pharmacopoeia (2015 edition).

[0034] Unless otherwise specified, the raw materials, reagents, etc. used in the following examples were obtained from commercial sources.

[0035] Example

[0036] The embodiments of the present invention provide an antibacterial and antiviral Chinese medicine composition. In each embodiment, the raw material composition of each dose is shown in Table 1 (unit: g).

[0037] Table 1 Raw material composition of the antibacterial and antiviral Chinese medicine composition of each embodiment

[0038] raw material Example 1 Example 2 Example 3 Example 4 Example 5 honeysuckle 9 8 10 7 12 Isatis root 15 13 17 11 18 Mianma Guanzhong 6 5 7 4 8 Patchouli 9 8 10 7 12 Anemarrhena 9 8 10 6 11 Ephedra 9 8 10 6 11 Burdock seeds 10 9 12 8 13 Peucedanum chinense 6 5 7 4 8 Rhodiola rosea 3 3 4 2 4

[0039] The embodiment of the present invention also provides a method for preparing the antibacterial and antiviral Chinese medicine composition in the above embodiment. The raw materials are weighed according to the raw material ratio in the antibacterial and antiviral Chinese medicine composition in the above embodiment, and the volatile oil of patchouli is extracted. The residue after the volatile oil is extracted from patchouli, as well as honeysuckle, isatis root, cyperus rotundus, anemarrhena, ephedra, arctium, peucedanum and rhodiola rosea are decocted 2 to 3 times, each time for 25 to 40 minutes, filtered to obtain a decoction filtrate, and the decoction filtrate is spray-dried at 55 to 65° C. to obtain a decoction extract; the volatile oil of patchouli is added to the obtained decoction extract and mixed evenly to obtain.

[0040] The specific parameter conditions in the preparation method of each embodiment are shown in Table 2.

[0041] Table 2 Preparation parameters of each example

[0042] parameter Example 1 Example 2 Example 3 Example 4 Example 5 Amount of water added for the first extraction (g) 480 470 540 480 680 First extraction time (min) 35 30 40 25 40 Amount of water added for the second extraction (g) 480 420 470 290 510 Second extraction time (min) 35 30 40 25 30 Amount of water added for the third extraction (g) / 420 / 290 / The third extraction time (min) / 30 / 25 /

[0043] Comparative Example 1

[0044] The comparative example of the present invention provides an antibacterial and antiviral Chinese medicine composition, based on the raw material composition of Example 1, removing Radix Isatidis, and increasing the amount of Flos Lonicerae, which has similar effects to Radix Isatidis and can clear away heat, detoxify, cool blood and reduce swelling. The raw materials in each dose are: Flos Lonicerae 24g, Rhizoma Cyrthospermi 6g, Pogostemon Cablin 9g, Rhizoma Anemarrhenae 9g, Ephedra 9g, Fructus Arctiae 10g, Peucedanum 6g and Rhodiola Rosea 3g. The preparation method is the same as that of Example 1.

[0045] Comparative Example 2

[0046] The comparative example of the present invention provides an antibacterial and antiviral Chinese medicine composition, based on the raw material composition of Example 1, burdock seeds are removed, and the amount of peucedanum chinense, which has similar effects to burdock seeds and can dissipate wind-heat and clear the lungs, is increased. The raw materials in each dose are: 9g of honeysuckle, 15g of isatis root, 6g of cyperus rotundus, 9g of patchouli, 9g of anemarrhena, 9g of ephedra, 16g of peucedanum chinense and 3g of rhodiola rosea. The preparation method is the same as that of Example 1.

[0047] Effect Example 1

[0048] This effect example provides a toxicity test of a single intragastric administration of the antibacterial and antiviral Chinese medicine composition to mice, in order to observe the possible toxic reactions after taking the antibacterial and antiviral Chinese medicine composition of the present invention.

[0049] 1. Materials

[0050] 1.1 Trial Drugs

[0051] The raw materials and preparation methods of Examples 1 to 5 were prepared, ground evenly, dissolved with 0.5% CMC-Na, and prepared into a concentration of 2 g crude drug / ml as the test solution.

[0052] 1.2 Experimental animals

[0053] ICR mice, SPF grade, 240 mice, 20-22 g, half male and half female, were purchased from Beijing Weitonglihua Laboratory Animal Technology Co., Ltd., license number: SCXK (Beijing) 2016-0011.

[0054] 2. Methods

[0055] 2.1 Grouping and Dosing

[0056] Male and female mice were randomly divided into 6 groups, 20 mice in each group, respectively, a blank control group and 5 example groups (example 1 group, example 2 group, example 3 group, example 4 group and example 5 group), each example group was gavaged with the test solution of examples 1 to 5 obtained in 1.1, the volume was 0.4ml / 10g body weight (maximum volume), and the administration was twice a day, with an interval of 6 hours, and the blank control group was given an equal amount of 0.5% CMC-Na. All animals were fasted overnight before administration, but not water.

[0057] 2.2 Observation indicators, time and content

[0058] After each administration, the animals were observed for 2 hours continuously, and the appearance, behavior, secretions, excretions, death and poisoning reactions (symptoms, starting time, severity, duration, reversibility of poisoning reactions) of the animals were observed, and the time of occurrence and duration of abnormal symptoms were recorded in detail. The animals were observed continuously for 14 days after administration, and the weight and food intake of the animals were recorded once a day on the 1st, 2nd, 3rd, 4th, 7th and 14th days after administration. After the observation period, the surviving animals were euthanized and grossly dissected. When changes in the volume, color, texture, etc. of the organs were found, the changed organs were subjected to histopathological examination.

[0059] 2.3 Data Analysis

[0060] The experimental data were statistically analyzed using SPSS 18.0 software, and the results were expressed as mean ± standard deviation. Indicates that independent samples two-sided T test was used.

[0061] 3. Results

[0062] 3.1 Impact on general condition

[0063] No abnormality was observed in the general conditions of the blank control group and each embodiment group.

[0064] 3.2 Effects on body weight and food intake

[0065] There was no significant statistical difference in the body weight of the male and female animals in each example group compared with the blank control group; there was no significant difference in the food intake compared with the blank control group. See Tables 3 to 6.

[0066] Table 3. Changes in body weight of male animals in the single oral administration toxicity test in ICR mice (g)

[0067]

[0068] Table 4. Body weight changes of female animals in the single oral administration toxicity test in ICR mice (g)

[0069]

[0070]

[0071] Table 5. Changes in food intake of male animals in the single oral administration toxicity test of ICR mice (g / day / piece)

[0072]

[0073] Table 6. Changes in food intake of female animals in the single oral administration toxicity test of ICR mice (g / day / piece)

[0074]

[0075] 3.3 Gross anatomy

[0076] After the observation period, the mice in the blank control group and each example group were subjected to gross anatomy, and no obvious abnormalities were found in the heart, liver, spleen, lung, kidney, brain, stomach, intestine, thymus, reproductive organs, bladder, etc.

[0077] 4. Conclusion

[0078] The maximum dosage for oral administration to mice was 160 g crude drug / kg, and no toxic reactions related to the test substance were observed at this dosage.

[0079] Effect Example 2

[0080] This effect example provides an experiment on the effect of the above antibacterial and antiviral Chinese medicine composition on LPS-induced fever in rats, so as to observe the antipyretic effect of taking the antibacterial and antiviral Chinese medicine composition of the present invention.

[0081] 1. Materials

[0082] 1.1 Trial Drugs

[0083] According to the raw material composition and preparation method of Examples 1 to 5 and Comparative Examples 1 and 2, the raw materials were prepared and ground evenly, dissolved with 0.5% CMC-Na, and prepared into a concentration of 0.3 g crude drug / ml as the test solution.

[0084] 1.2 Experimental animals

[0085] SD rats, SPF grade, 96, 180-200 g, male, were purchased from Beijing Weitonglihua Laboratory Animal Technology Co., Ltd., license number: SCXK (Beijing) 2016-0011.

[0086] 2. Methods

[0087] 2.1 Grouping and Dosing

[0088] Rats were randomly divided into 8 groups, each with 12 rats, including a model control group, 5 example groups (example 1 group, example 2 group, example 3 group, example 4 group and example 5 group), comparative example 1 group and comparative example 2 group. Each example group was gavaged with the test solution of examples 1 to 5 obtained in 1.1, and the comparative example 1 group and comparative example 2 group were gavaged with the test solution of comparative examples 1 and 2 obtained in 1.1, respectively, with the gavage volume of 1 ml / 100 g body weight. The model control group was given an equal amount of 0.5% CMC-Na. After 30 minutes of administration, the rectal temperature of each group of rats was measured once and recorded as T 0 Then, 20 μg / kg LPS (prepared with saline) was injected intraperitoneally to establish the model, with a volume of 5 ml / kg. The rectal temperature was measured every 1 hour after model establishment and recorded as T x , for 5 consecutive hours, calculate the body temperature difference △T, △T = T x -T 0 .

[0089] 2.2 Data Analysis

[0090] The experimental data were statistically analyzed using SPSS 18.0 software, and the results were expressed as mean ± standard deviation. Indicated by one-way analysis of variance.

[0091] 3. Results

[0092] The body temperature difference of the rats in the model control group showed a significant upward trend 1-5 hours after the model was established by intraperitoneal injection of LPS. Compared with the model control group, the body temperature difference of the rats in the embodiment group was significantly reduced 2 hours and 3 hours after the model was established (P<0.05), and the body temperature difference of the rats in the comparative example group was significantly reduced 3 hours after the model was established (P<0.05). See Table 7.

[0093] Table 7 Effects of LPS-induced fever in rats

[0094]

[0095]

[0096] Note: Compared with the model control group: *P<0.05.

[0097] 4. Conclusion

[0098] The antibacterial and antiviral Chinese medicinal composition of the present invention has the effect of inhibiting fever caused by intraperitoneal injection of LPS in rats, and the antibacterial and antiviral Chinese medicinal composition provided by the embodiment of the present invention is superior to the comparative example.

[0099] Effect Example 3

[0100] This effect example investigates the antibacterial and antiviral Chinese medicine composition's antibacterial effect on Gram-positive bacteria and Gram-negative bacteria to observe the antibacterial effect of the antibacterial and antiviral Chinese medicine composition of the present invention.

[0101] 1. Materials

[0102] 1.1 Trial Drugs

[0103] The raw materials and preparation methods of Examples 1 to 5 and Comparative Examples 1 and 2 were prepared, ground evenly, dissolved with dimethyl sulfoxide (DMSO) and ultrasonically assisted to prepare a concentration of 500 mg crude drug / ml, and filtered with a 0.22 μm microporous filter membrane to serve as the test solution.

[0104] 1.2 Test strains

[0105] Gram-positive bacteria: Staphylococcus aureus (ATCC6538), Streptococcus pyogenes (ATCC19615);

[0106] Gram-negative bacteria: Escherichia coli (ATCC35218), Pseudomonas aeruginosa (ATCC9027).

[0107] 1.3 Culture medium

[0108] Beef extract peptone solid medium: 0.5 g beef extract, 0.5 g sodium chloride, 1.0 g peptone, 100 mL water, pH 7.0-7.2. Sterilize at 121°C, 0.1 MPa for 20 min.

[0109] Beef extract peptone liquid culture medium: 0.5g beef extract, 0.5g sodium chloride, 1.0g peptone, 2.0-4.0g agar powder, 100mL water, pH 7.0-7.2. Sterilize at 121℃, 0.1MPa for 20min.

[0110] 2. Methods

[0111] The 5th generation nutrient agar slant culture (24 h) of the test strain was washed with sterile water and diluted with the above beef extract peptone liquid medium to prepare a 10 6 cfu / ml bacterial suspension.

[0112] The test liquids of each embodiment and comparative example are operated as follows: Take a sterilized 96-well plate, and add 100 μL of beef extract peptone liquid culture medium to the 1st to 11th wells respectively. Take 100 μL of the test liquid and add it to the 1st well. After mixing evenly, draw 100 μL from the 1st well and add it to the 2nd well. After mixing evenly, draw 100 μL from the 2nd well and add it to the 3rd well. Do this until the 10th well, and finally draw 100 μL from the 10th well and discard it. Take 100 μL of bacterial suspension and add it to the 1st to 12th wells of the 96-well plate. Add 100 μL of dimethyl sulfoxide to the 12th well as a negative control. Three parallel tests are set for each of the above groups.

[0113] 3. Results

[0114] The above 96-well plate was placed in a 37°C constant temperature incubator for 24 hours and then observed against a black background to obtain the minimum inhibitory concentration of each sample. The results are shown in Table 8.

[0115] Table 8 Minimum inhibitory concentration of samples (based on crude drugs)

[0116]

[0117] 4. Conclusion

[0118] The antibacterial and antiviral Chinese medicinal composition of the present invention has an inhibitory effect on both Gram-positive bacteria and Gram-negative bacteria, and the antibacterial and antiviral Chinese medicinal composition provided by the embodiment of the present invention is superior to the comparative example.

[0119] Effect Example 4

[0120] This effect example provides the effect of the above antibacterial and antiviral Chinese medicine composition on influenza A (H1N1) virus in vitro, so as to observe the antiviral effect of taking the antibacterial and antiviral Chinese medicine composition of the present invention.

[0121] 1. Materials

[0122] 1.1 Trial Drugs

[0123] According to the raw materials and preparation methods of Example 1 and Comparative Example 1, the mixtures were ground evenly, dissolved with dimethyl sulfoxide (DMSO) and ultrasonically assisted, concentrated to the required concentration, centrifuged, and filtered to obtain the supernatant for later use as the test solution.

[0124] 1.2 Cells

[0125] VeroE6 cells are maintained by the Virus Laboratory of the State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Health.

[0126] 1.3 Viruses

[0127] Influenza A (H1N1) virus, titer is TCID 50 =10-3.5 , maintained by the Influenza Room of the Institute of Viral Disease Prevention and Control, Chinese Center for Disease Control and Prevention.

[0128] 2. Methods

[0129] 2.1 MTT assay to determine in vitro toxicity

[0130] VeroE6 cells were seeded in 96-well plates, and 100 μL of 2×10 5 cells / mL of VeroE6 cells, set up 4 replicate wells, 37℃ 5% CO 2 After the cells grew into a monolayer, the cells were divided into a blank group (without cells, only culture medium), a solvent control group (DMSO group), Example 1 group and Comparative Example 1 group. 100uL of VeroE6 cell culture medium was added to the blank group, 100uL of DMSO was added to the solvent control group, different concentrations of the test solution of Example 1 were added to the Example 1 group, and different concentrations of the test solution of Comparative Example 1 were added to the Comparative Example 1 group. The cells in each group were incubated at 5% CO 2 , incubate at 37°C for 48 hours, add 100uL 1mg / ml MTT solution, continue to culture for 4h, then terminate the culture, add 100uL DMSO to each well, and shake at low speed on a shaker for 10 minutes. Measure the absorbance of each well at 490nm on a full wavelength scanner, and calculate the TC of the drug using GraphPad Prism6.0 50 value.

[0131] 2.2 In vitro anti-influenza virus activity assay

[0132] The antiviral activity of the drug was tested by cytopathic inhibition assay. VeroE6 cells were seeded in 96-well plates, and 100 μL of 2×10 5 cells / ml of VeroE6 cells, set up 4 replicate wells, 37℃ 5% CO 2 The cells were cultured until they grew into a monolayer, the culture medium was discarded, and the cell surface was washed twice with PBS. The cells were divided into a blank group, a solvent control group, a virus control (negative control) group, an Example 1 group, and a Comparative Example 1 group. 100 TCID 50100uL / well of influenza A (H1N1) virus dilution was added and incubated in an incubator. After 2 hours, the virus solution was discarded and the medium containing the drug to be tested was added. The cells were incubated in the incubator for 48 hours. The cell lesions were observed and recorded every day. The degree of cell lesions was recorded according to the following 6-level standards: "-" no lesions; "±" less than 10% cell lesions; "+" about 25% cell lesions; "++" about 50% cell lesions; "+++" about 75% of cells had lesions: "++++" more than 75% lesions. The half inhibitory concentration (IC) was calculated using GraphPad Prism6.0 50 ).

[0133] 2.3 Data Analysis

[0134] Calculate the selection index SI, SI = TC 50 / IC 50 .

[0135] 3. Results

[0136] 3.1 Results of toxicity experiments on VeroE6 cells

[0137] Effect of the antibacterial and antiviral Chinese medicine composition obtained in Example 1 of the present invention on TC of VeroE6 cells 50 The antibacterial and antiviral Chinese medicine composition obtained in Comparative Example 1 had an effect on the TC of VeroE6 cells. 50 It is 4097μg / ml, see Table 9 and Table 10.

[0138] Table 9 Toxicity of the antibacterial and antiviral Chinese medicine composition obtained in Example 1 to VeroE6 cells

[0139]

[0140] Table 10 Toxicity of the antibacterial and antiviral Chinese medicine composition obtained in Comparative Example 1 to VeroE6 cells

[0141]

[0142] 3.2 Experimental results on influenza A (H1N1) virus

[0143] IC of the antibacterial and antiviral Chinese medicine composition obtained in Example 1 of the present invention against influenza A (H1N1) virus 50 is 106.6μg / ml, after calculation, SI=TC 50 / IC 50 =57.9. IC of the antibacterial and antiviral Chinese medicine composition obtained in Comparative Example 1 against influenza virus A (H1N1) 50 It is 169.9μg / ml. After calculation, SI = TC 50 / IC 50=24.1. See Table 11 and Table 12.

[0144] Table 11 Effects of the antibacterial and antiviral Chinese medicine composition obtained in Example 1 on influenza virus A (H1N1)

[0145]

[0146] Table 12 Effect of the antibacterial and antiviral Chinese medicine composition obtained in Comparative Example 1 on influenza virus A (H1N1)

[0147]

[0148] Effect Example 5

[0149] This effect example provides the clinical application effect of the antibacterial and antiviral Chinese medicine composition of Example 1.

[0150] 1. Cases and treatment methods

[0151] The clinical application effects of 112 patients with the same symptoms who took the antibacterial and antiviral Chinese medicine composition of Example 1 of the present invention every summer from May 2018 to July 2020 were statistically analyzed.

[0152] The average age of the above patients was 33.7 years old (age range 16-46 years old), and the clinical symptoms were sore throat, fever, aversion to cold, thirst, dry mouth, chest tightness, white tongue coating, bright red tongue, and rapid pulse. Each person took one dose of the antibacterial and antiviral Chinese medicine composition provided in Example 1 of the present invention every day, and no other drugs were used during the treatment period. The work and rest and diet were regular, no staying up late, light diet, and no direct exposure to air conditioning.

[0153] 2. Treatment effect

[0154] The cure standard is the disappearance of all the above clinical symptoms.

[0155] According to statistics, the body temperature of the above patients dropped after taking the medicine for 1 to 2 days, sore throat and aversion to cold were significantly relieved after 3 to 5 days, and all the above clinical symptoms disappeared after 6 to 10 days, with a cure rate of 100%. All patients had no side effects or adverse reactions during the treatment.

[0156] This result shows that the antibacterial and antiviral Chinese medicine composition provided by the present invention can effectively eliminate the above clinical symptoms of patients.

[0157] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibacterial and antiviral Chinese medicine composition, characterized in that: The invention is prepared from the following raw materials in proportion by weight: 7-12 parts of honeysuckle, 11-18 parts of isatis root, 4-8 parts of cyperus rotundus, 7-12 parts of patchouli, 6-11 parts of anemarrhena, 6-11 parts of ephedra, 8-13 parts of burdock fruit, 4-8 parts of peucedanum and 2-4 parts of rhodiola rosea.

2. The antibacterial and antiviral Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following raw materials in proportion by weight: 8-10 parts of honeysuckle, 13-17 parts of isatis root, 5-7 parts of cyperus rotundus, 8-10 parts of patchouli, 8-10 parts of anemarrhena, 8-10 parts of ephedra, 9-12 parts of burdock fruit, 5-7 parts of peucedanum and 3-4 parts of rhodiola rosea.

3. The antibacterial and antiviral Chinese medicine composition according to claim 2, characterized in that: The invention is prepared from the following raw materials in proportion by weight: 9 parts of honeysuckle, 15 parts of isatis root, 6 parts of cyperus rotundus, 9 parts of patchouli, 9 parts of anemarrhena, 9 parts of ephedra, 10 parts of burdock fruit, 6 parts of peucedanum and 3 parts of rhodiola rosea.

4. The method for preparing the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The method comprises the following operations: weighing each raw material according to the raw material ratio in the antibacterial and antiviral Chinese medicine composition, extracting the volatile oil of the patchouli, decocting the residue with the honeysuckle, isatis root, cyperus rotundus, rhizoma anemarrhenae, ephedra, arctium fruit, peucedanum and rhodiola rosea, filtering the decocted extract, collecting the obtained filtrate, drying, and obtaining a decoction extract; adding the volatile oil of the patchouli to the obtained decoction extract and mixing evenly, and obtaining the antibacterial and antiviral Chinese medicine composition.

5. Use of the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicine for treating sore throat.

6. Use of the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3 in the preparation of antipyretic drugs.

7. Use of the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicine for treating sore throat combined with fever or chills.

8. Use of the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicine for treating sore throat combined with fever and chills.

9. Use of the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicament for treating at least one of sore throat, fever, aversion to cold combined with dry mouth, and chest tightness.

10. An antiviral and / or antibacterial drug, characterized in that: The medicine is prepared from the antibacterial and antiviral Chinese medicine composition according to any one of claims 1 to 3.

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