An instrument for detecting plant cell turgor pressure and membrane potential

Abstract

The present invention proposes an instrument for detecting plant cell turgor pressure and membrane potential, comprising a three-way mounting base; a sample injector disposed on a first interface of the three-way mounting base; an interface base disposed on a second interface of the three-way mounting base; a pressure sensor disposed on a third interface of the three-way mounting base; a Luer connector disposed at an end of the interface base remote from the three-way mounting base; a capillary glass tube disposed on the Luer connector; a first electrode wire disposed within the capillary glass tube; and a second electrode wire disposed on the interface base. The interior of the three-way mounting base, the interior of the sample injector, the interior of the interface base, and the interior of the capillary glass tube are connected by dielectric oil. The present application can simultaneously measure plant cell turgor pressure and membrane potential, is simple, convenient, and highly practical, and eliminates the need for frequent replacement of detection instruments in actual testing.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cell detection instruments, in particular to an instrument for detecting turgor pressure and membrane potential of plant cells. Background Art

[0002] Turgor pressure refers to the pressure exerted on the cell wall by plant cells due to their volume expansion caused by water absorption. During initial plasmolysis, the protoplasts separate from the cell wall, exerting no pressure on the cell wall. At this point, the turgor pressure in the plant cell is zero, indicating a state of no tension. If the cell is placed in water, water flows into the cell along the water's potential, increasing the water content of the vacuole and its volume. This increases the turgor pressure of the plant cell. When the cell reaches saturation with water, the turgor pressure reaches its maximum value, indicating a state of maximum tension. Therefore, turgor pressure is the cause of plant cell tension, and its value directly reflects the degree of cellular tension.

[0003] Membrane potential refers to the potential difference between the inside and outside of a plant cell membrane. When a plant cell is at rest, positive charges reside on the outside of the membrane (positive potential on the outside) and negative charges reside on the inside (negative potential on the inside). This state is called polarization. If the potential difference between the inside and outside of the membrane increases, that is, the resting potential changes toward a more negative value on the inside, this is called hyperpolarization. Conversely, if the potential difference between the inside and outside of the membrane decreases, that is, the potential on the inside changes toward a less negative value, this is called depolarization or polarization. When a plant cell is stimulated, a potential change can occur relative to the resting potential. This potential change is called an action potential. The waveform of an action potential varies depending on the recording method. When a microelectrode is placed inside the cell, rapid, reversible changes are recorded, manifesting as a spike potential. The spike potential represents the process of cellular excitation and is a marker of the generation and conduction of excitation.

[0004] At present, the detection instruments on the market can only detect the turgor pressure of plant cells or the membrane Potential, the two cannot be carried out at the same time. In actual detection, it is necessary to frequently replace the detection instruments, which is cumbersome, time-consuming and labor-intensive. Summary of the Invention

[0005] Based on this, the present invention provides an instrument for detecting the turgor pressure and membrane potential of plant cells, which aims to solve the problem that the detection instruments on the market can only detect the turgor pressure of plant cells or the membrane potential of plant cells. membrane Potential, the two cannot be carried out at the same time, and in actual testing, the testing instruments need to be frequently replaced, which is cumbersome, time-consuming and labor-intensive.

[0006] To achieve the above object, the present invention proposes the following technical solutions:

[0007] An instrument for detecting plant cell turgor pressure and membrane potential comprises a three-way fixing seat; a sample injector is provided on a first interface of the three-way fixing seat; an interface seat is provided on a second interface of the three-way fixing seat; a pressure sensor is provided on a third interface of the three-way fixing seat; a Luer connector is provided at one end of the interface seat away from the three-way fixing seat; a capillary glass tube is provided on the Luer connector; a first electrode wire is provided in the capillary glass tube; a second electrode wire is also provided on the interface seat; the interior of the three-way fixing seat, the interior of the sample injector, the interior of the interface seat, and the interior of the capillary glass tube are connected by dielectric oil.

[0008] Furthermore, the injector includes an outer tube, a push rod and a push piston; a first through hole and a second through hole connected in sequence are provided in the outer tube along the axial direction of the outer tube; the end of the first through hole away from the second through hole is connected to the first interface of the three-way fixing seat; one end of the push rod is slidably provided in the second through hole, and the other end extends outward; the push piston is provided at the end of the push rod close to the first through hole.

[0009] Furthermore, the aperture of the first through hole is smaller than the aperture of the second through hole.

[0010] Furthermore, the interface seat is provided with a third through hole and a fourth through hole which are connected in sequence; the end of the third through hole away from the fourth through hole is connected to the second interface of the three-way fixing seat; the end of the fourth through hole away from the third through hole is connected to the Luer connector.

[0011] Furthermore, the aperture of the third through hole is larger than the aperture of the fourth through hole.

[0012] Furthermore, the interface seat is provided with a mounting hole for mounting the second electrode.

[0013] Furthermore, a square groove is provided on the side surface of the three-way fixing seat.

[0014] Furthermore, the bottom of the three-way fixing seat is also provided with a plurality of screw holes for fixing.

[0015] Furthermore, the pressure sensor includes a threaded joint, a housing, and a lead wire that are arranged in sequence; the threaded joint is connected to the third joint of the three-way fixing seat.

[0016] The present invention proposes an instrument for detecting plant cell turgor pressure and membrane potential. By installing a first electrode wire within a capillary glass tube and a second electrode wire on an interface holder, the instrument can accurately measure the membrane potential of plant cells. Adjusting the push rod on the injector effectively adjusts the interface position of the medium oil within the capillary glass tube near the cell side, thereby improving the accuracy of plant cell turgor pressure detection. This application can simultaneously measure plant cell turgor pressure and membrane potential, offering simplicity, convenience, and high practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0018] Figure 1 This is a three-dimensional structural diagram of an instrument for detecting plant cell turgor pressure and membrane potential according to an embodiment of the present invention;

[0019] Figure 2 for Figure 1 Three-dimensional structural diagram of the middle three-way fixing seat;

[0020] Figure 3 for Figure 1 The three-dimensional structure diagram of the middle sampler;

[0021] Figure 4 for Figure 1 A three-dimensional structural diagram of the middle interface seat (including the Luer connector and capillary glass tube);

[0022] Figure 5 for Figure 1 Three-dimensional structure diagram of the medium pressure sensor.

[0023] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0025] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, top, bottom...), then the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0026] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0027] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element.

[0028] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features specified as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that ordinary technicians in this field can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0029] Currently, commercially available instruments can only measure either plant cell turgor pressure or plant cell membrane potential, but not both simultaneously. This requires frequent instrument changes during practical testing, which is cumbersome, time-consuming, and labor-intensive. To address these technical issues, the present invention proposes an instrument for measuring both plant cell turgor pressure and membrane potential.

[0030] like Figure 1 and Figure 2As shown, an embodiment of the present invention proposes an instrument for detecting plant cell turgor pressure and membrane potential, comprising a three-way fixing seat 1; a sample injector 2 is provided on the first interface 11 of the three-way fixing seat 1; an interface seat 3 is provided on the second interface 12 of the three-way fixing seat 1; a pressure sensor 4 is provided on the third interface 13 of the three-way fixing seat 1; a Luer connector 5 is provided at one end of the interface seat 3 away from the three-way fixing seat 1; a capillary glass tube 6 is provided on the Luer connector 5; a first electrode wire (not shown in the figure) is provided in the capillary glass tube 6; a second electrode wire (not shown in the figure) is also provided on the interface seat 3; the interior of the three-way fixing seat 1, the interior of the sample injector 2, the interior of the interface seat 3, and the interior of the capillary glass tube 6 are connected by dielectric oil (not shown in the figure). It can be understood that during the detection process, the capillary glass tube 6 is inserted into the cell, so that the first electrode can reflect the potential inside the cell membrane, and the second electrode on the interface seat is outside the cell membrane. In actual application, the first electrode and the second electrode can be connected to the AD converter, and after AD conversion, they are directly sent to the PC for display processing, so that the membrane potential of the cell can be accurately measured; and, dielectric oil is provided in the capillary glass tube 6, and the dielectric oil can reflect the pressure inside the cell to the pressure sensor 4, thereby measuring the turgor pressure of the cell.

[0031] Reference Figure 3 In some embodiments, the injector 2 includes an outer tube 21, a push rod 22, and a push piston 23. A first through hole 211 and a second through hole 212 are sequentially connected within the outer tube 21 along its axis. The first through hole 211, distal to the second through hole 212, is connected to the first interface 11 of the three-way fixture 1. One end of the push rod 22 is slidably disposed within the second through hole 212, while the other end extends outward. The push piston 23 is disposed at the end of the push rod 22 proximal to the first through hole 211. It will be appreciated that the push rod 22 adjusts the interface position of the medium oil within the second through hole 212 by pushing the piston 23, thereby adjusting the interface position of the medium oil on the cell-proximal side of the capillary glass tube 6, thereby accurately detecting cell turgor pressure.

[0032] In some embodiments, the diameter of the first through hole 211 is smaller than the diameter of the second through hole 212. It is understood that this can more accurately control the flow of the medium oil from the second through hole 212 to the first through hole 211, thereby facilitating accurate adjustment of the interface position of the medium oil.

[0033] Reference Figure 4In some embodiments, the interface base 3 is provided with a third through hole 31 and a fourth through hole 32, which are connected in sequence. The end of the third through hole 31 remote from the fourth through hole 32 is connected to the second interface 12 of the three-way fixing base 1, and the end of the fourth through hole 32 remote from the third through hole 31 is connected to the Luer connector 5. It is understood that the medium oil can flow from the third through hole 31 through the fourth through hole 34 to the Luer connector 5, and then through the Luer connector 5 to the capillary glass tube 6 for detecting the cell turgor pressure.

[0034] In some embodiments, the diameter of the third through hole 31 is larger than the diameter of the fourth through hole 32. It is understood that this can more accurately control the flow of the medium oil from the third through hole 31 to the fourth through hole 32, thereby facilitating accurate adjustment of the interface position of the medium oil.

[0035] In some embodiments, the interface base 3 is further provided with a mounting hole 33 for mounting the second electrode. It is understood that the second electrode is mounted on the interface base 3 via the mounting hole 33. In practical applications, the second electrode is preferably a 2 mm banana connector, with the electrode terminal of the banana connector connected to the mounting hole 33 and positioned outside the cell membrane, thereby cooperating with the first electrode to measure the cell membrane potential.

[0036] Reference Figure 2 In some embodiments, a square groove 14 is further provided on the side of the three-way fixing seat 1. The square groove 14 is convenient for providing sufficient space when connecting a banana connector.

[0037] Refer again Figure 2 In some embodiments, the bottom of the three-way fixing seat 1 is further provided with a plurality of screw holes 15 for fixing. It is understandable that the screw holes 15 facilitate the use of screws to fix the three-way fixing seat 1 on a manual micromanipulator or an electric micromanipulator for easy use.

[0038] Reference Figure 5 In some embodiments, the pressure sensor 4 includes a threaded connector 41, a housing 42, and a lead wire 43, which are sequentially arranged. The threaded connector 41 is connected to the third connector 13 of the three-way mounting base 1. It is understood that the lead wire 43 can transmit the pressure data of the medium oil inside the three-way mounting base 1 to a high-precision analog-to-digital converter, which then transmits the pressure data to a PC, where various data are ultimately displayed and processed. The pressure sensor can use a DC 5V pressure sensor with a range of 0 to 1 MPa.

[0039] This application proposes an instrument for detecting plant cell turgor pressure and membrane potential. The detection principle is as follows:

[0040] Principle of detecting plant cell membrane potential: First, use screws to fix the three-way fixing base 1 and the various components connected to the three-way fixing base 1 on the micromanipulator, and control the micromanipulator to pierce the capillary glass tube 6 into the inner membrane of the plant cell; at this time, the first electrode in the capillary glass tube 6 is also in the inner membrane of the plant cell, and the second electrode on the interface base 3 is in the external fluid of the plant cell. Then, the first electrode and the second electrode are connected to the AD converter, and after AD conversion, they are directly sent to the PC for display processing, so that the membrane potential of the cell can be accurately measured.

[0041] Principle of detecting plant cell turgor pressure: The capillary glass tube 6 is inserted into the inner membrane of the plant cell by controlling the micromanipulator; at this time, the medium oil in the capillary glass tube 6 contacts the cytoplasm in the plant cell membrane. The cytoplasm of the plant cell will generate pressure on the medium oil, and the medium oil will transmit the pressure to the pressure sensor 4. The pressure sensor 4 is connected to a high-precision AD converter through the lead wire 43, and the pressure data is then sent to the PC end through the AD converter. Finally, various data are displayed and processed on the PC end; before the entire detection, it is necessary to adjust the interface position of the medium oil on the side close to the plant cell in the capillary glass tube 6 through the injector 2 to ensure accurate measurement of the plant cell turgor pressure.

[0042] An instrument for detecting plant cell turgor pressure and membrane potential, proposed in an embodiment of the present invention, accurately measures plant cell membrane potential by installing a first electrode wire within a capillary glass tube 6 and a second electrode wire on an interface base 3. Adjusting the push rod 22 on the injector 2 effectively adjusts the interface position of the medium oil within the capillary glass tube 3 near the cell side, thereby improving the accuracy of plant cell turgor pressure detection. This device can simultaneously measure plant cell turgor pressure and membrane potential, offering simplicity, convenience, and high practicality.

[0043] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention description and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. An instrument for detecting plant cell turgor pressure and membrane potential, characterized in that: The invention comprises a three-way fixing seat; a sample injector is provided on a first interface of the three-way fixing seat; an interface seat is provided on a second interface of the three-way fixing seat; a pressure sensor is provided on a third interface of the three-way fixing seat; a Luer connector is provided on an end of the interface seat away from the three-way fixing seat; a capillary glass tube is provided on the Luer connector; a first electrode wire is provided in the capillary glass tube; a second electrode wire is also provided on the interface seat; the interior of the three-way fixing seat, the interior of the sample injector, the interior of the interface seat, and the interior of the capillary glass tube are connected by dielectric oil; The second electrode is a banana connector; When measuring the cell membrane potential, the first electrode in the capillary glass tube is located in the inner membrane of the plant cell, and the second electrode on the interface seat is located in the outer fluid of the plant cell; The interface seat is provided with a third through hole and a fourth through hole which are connected in sequence; the end of the third through hole away from the fourth through hole is connected to the second interface of the three-way fixing seat; the end of the fourth through hole away from the third through hole is connected to the Luer connector; The aperture of the third through hole is larger than the aperture of the fourth through hole.

2. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 1, characterized in that: The injector includes an outer tube, a push rod and a push piston; a first through hole and a second through hole connected in sequence are provided in the outer tube along the axial direction of the outer tube; the end of the first through hole away from the second through hole is connected to the first interface of the three-way fixing seat; one end of the push rod is slidably set in the second through hole, and the other end extends outward; the push piston is set at the end of the push rod close to the first through hole.

3. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 2, wherein: The aperture of the first through hole is smaller than the aperture of the second through hole.

4. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 1, wherein: The interface seat is also provided with a mounting hole for mounting the second electrode.

5. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 1, wherein: A square groove is also provided on the side of the three-way fixing seat.

6. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 1, wherein: The bottom of the three-way fixing seat is also provided with a plurality of screw holes for fixing.

7. The instrument for detecting plant cell turgor pressure and membrane potential according to claim 1, wherein: The pressure sensor includes a threaded joint, a shell and a lead wire which are arranged in sequence; the threaded joint is connected to the third joint of the three-way fixing seat.

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