A culture device for downregulating dox-induced autophagy in cardiomyocytes

By designing a culture device including shaking and fixing mechanisms, dynamic culture of cardiomyocytes is achieved, and uneven consumption of nutrient solution and liquid leakage caused by static culture is solved, thereby improving cell density and growth conditions.

CN114717115BActive Publication Date: 2024-12-10HANGZHOU HUASHEN HEALTH IND CO LTD
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Patent Information

Application Number
CN202210494317.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-05-07
Publication Date
2024-12-10
Estimated Expiration
2042-05-07

AI Technical Summary

Technical Problem

The static placement of existing cardiomyocyte culture devices leads to unbalanced consumption of nutrient solution, limiting the proliferation of cells, and failing to achieve dynamic culture, which in turn limits the increase in cell density.

Method used

A culture device including a base plate, mounting plate, shaking mechanism, fixing mechanism, locking mechanism and triggering mechanism is designed. Through the reciprocating sliding of the mounting plate and the control of the pressing plate, the shaking and cap of the culture dish are realized to ensure dynamic culture of cells. And prevent liquid leakage.

Benefits of technology

Dynamic culture of cardiomyocytes is achieved, cell density is improved, and uneven consumption of culture medium and liquid leakage is avoided, thereby enhancing the conditions for cell growth.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a culture device for down-regulating the autophagy function of cardiomyocytes induced by dox, and belongs to the field of cardiomyocyte culture. The device comprises a bottom plate, a mounting plate, a shaking mechanism, a fixing mechanism, a locking mechanism and a triggering mechanism. The shaking mechanism is arranged on the bottom plate to drive the reciprocating sliding of the mounting plate, thereby realizing the shaking of the culture dish and realizing the dynamic culture of the cardiomyocytes. The fixing mechanism is arranged on the upper end of the mounting plate to fix the culture dish, and the upper end of the culture dish is sealed to prevent leakage during shaking. The locking mechanism is arranged on the upper end of the bottom plate to limit the operation of the shaking mechanism, and the triggering mechanism is arranged to control the locking timing of the locking mechanism, thereby controlling the sliding timing of the shaking mechanism. The shaking mechanism can only be operated when the pressing plate presses and fixes the culture dish, thereby preventing the leakage of the liquid in the culture dish, instead of relying solely on controlling the reciprocating sliding timing of the mounting plate by controlling a first driving device.
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Description

Technical Field

[0001] The invention belongs to the field of cardiomyocyte culture, and in particular relates to a culture device for down-regulating the autophagy function of cardiomyocytes induced by dox. Background Art

[0002] Cardiomyocytes are long-lived late-mitotic cells with very limited differentiation and regeneration capabilities. Autophagy provides energy to cells, promotes material circulation, and promotes cell self-renewal by degrading dysfunctional or misfolded proteins and damaged or aged organelles.

[0003] Currently, most of the cardiomyocytes cultured in laboratories use culture plates or culture bottles as culture devices, and are placed statically during the culture process. During cell growth, the culture medium is static, resulting in uneven consumption of the nutrient solution, which in turn limits cell proliferation. Therefore, in order to obtain a higher cell density, it is necessary to make the cardiomyocyte culture dynamic. Summary of the invention

[0004] In view of the deficiencies of the prior art, the object of the present invention is to provide a culture device for down-regulating the autophagy function of cardiomyocytes induced by dox, thereby realizing the dynamic culture of cardiomyocytes.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A culture device for down-regulating the autophagy function of cardiomyocytes induced by dox, comprising a bottom plate, the upper end of which is slidably connected with a mounting plate, the upper end of which is provided with a plurality of positioning grooves, and a culture dish is mounted in the positioning grooves;

[0007] A shaking mechanism is arranged at the upper end of the base plate, and the shaking mechanism includes a first driving device fixed on the upper end of the base plate, a first connecting rod is fixed on the driving shaft of the first driving device, one end of the first connecting rod is rotatably connected to the second connecting rod, a connecting seat is arranged on one side of the mounting plate, one end of the second connecting rod is hinged to the connecting seat, and by controlling the operation of the first driving device, the mounting plate can be realized to slide back and forth on the upper end of the base plate.

[0008] Further, a fixing mechanism is provided at the upper end of the mounting plate, the fixing mechanism includes a fixing frame fixed to the upper end of the mounting plate, a first rack is slidably connected to the fixing frame, a pressing plate is fixed to the lower end of the first rack, a second driving device is provided on the fixing frame, a first gear is fixed to the driving shaft of the second driving device, and the first gear and the first rack are meshed with each other;

[0009] By controlling the pressing plate to descend, the culture dish can be pressed tightly in the positioning groove, and at the same time, the pressing plate can cover the upper end of the culture dish.

[0010] Furthermore, a locking mechanism is provided at the upper end of the bottom plate, and the locking mechanism is used to limit the movement of the shaking mechanism;

[0011] The locking mechanism includes a first fixed plate fixed to the upper end of the base plate, a sliding rod slidably connected to the first fixed plate, a clamping plate is provided at one end of the sliding rod, a second gear is fixed to the driving shaft of the first driving device, a spring is sleeved on the sliding rod, two ends of the spring are respectively fixedly connected to the side surface of the first fixed plate and the side surface of the clamping plate, and under the elastic force of the spring, the end of the clamping plate can be engaged with two adjacent teeth of the second gear.

[0012] Furthermore, the sliding rod is provided with a second rack, the first fixed plate is rotatably connected with a rotating shaft, a third gear and a fourth gear are fixed on the rotating shaft, and the third gear is meshed with the second rack;

[0013] The bottom plate is provided with a trigger mechanism, which includes a support plate and a connecting rod. The support plate is fixed to the upper end of the bottom plate. The support plate is provided with a T-shaped slide groove in the vertical direction. One end of the connecting rod cooperates with the T-shaped slide groove and is slidably connected. The sliding direction is the vertical direction. A second fixed plate is provided on one side of the first rack. The connecting rod penetrates the second fixed plate and is slidably connected to the second fixed plate. When the first rack is lifted or lowered, the connecting rod can be driven to rise or fall synchronously, and the connecting rod will not interfere with the reciprocating sliding of the mounting plate.

[0014] A third rack is provided at one end of the connecting rod. When the connecting rod descends along with the first rack, the third rack can mesh with the fourth gear and drive the sliding rod to slide in a direction away from the second gear.

[0015] Furthermore, when the pressing plate presses the culture dish into the positioning groove, the clamping plate just leaves the second gear.

[0016] Furthermore, a lifting rod is fixed to the upper end of the third rack, the lifting rod passes through the connecting rod along the vertical direction and is slidably connected to the connecting rod, a support frame is fixed on the connecting rod, an adjusting rod is threadedly connected to the support frame, and the lower end of the adjusting rod is rotatably connected to the lifting rod. By twisting the adjusting rod, the lifting and lowering movement of the lifting rod can be controlled, thereby realizing the adjustment of the vertical position of the third rack relative to the connecting rod.

[0017] The beneficial effects of the present invention are as follows: the culture dish is installed on the mounting plate by slidingly connecting the mounting plate on the upper end of the bottom plate, and a shaking mechanism is provided to drive the mounting plate to slide back and forth, thereby realizing the shaking of the culture dish and realizing the dynamic culture of myocardial cells; the culture dish is fixed by providing a fixing mechanism on the upper end of the mounting plate, and the upper end of the culture dish is sealed to prevent leakage during shaking; the operation of the shaking mechanism is limited by providing a locking mechanism on the upper end of the bottom plate, and the locking timing of the locking mechanism is controlled by providing a trigger mechanism, thereby controlling the sliding timing of the shaking mechanism, and the shaking mechanism can only operate when the pressing plate presses and fixes the culture dish, thereby avoiding leakage of liquid in the culture dish, instead of relying solely on controlling the first driving device to control the reciprocating sliding timing of the mounting plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 is a schematic diagram of the overall structure of an embodiment of the present invention;

[0020] Figure 2 is a schematic diagram of the structure of a mounting plate according to an embodiment of the present invention;

[0021] Figure 3 is a schematic structural diagram of a shaking mechanism according to an embodiment of the present invention;

[0022] Figure 4 is a schematic diagram of the fixing mechanism structure of an embodiment of the present invention;

[0023] Figure 5 is a schematic diagram of the first rack structure of an embodiment of the present invention;

[0024] Figure 6 is a schematic structural diagram of a locking mechanism according to an embodiment of the present invention;

[0025] Figure 7 is a schematic diagram of the sliding rod structure of an embodiment of the present invention;

[0026] Figure 8 is a schematic diagram of the structure of a trigger mechanism according to an embodiment of the present invention;

[0027] Fig. 9 It is an enlarged schematic diagram of the structure at location A of an embodiment of the present invention. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] like Figure 1-3 As shown, a culture device for down-regulating dox-induced cardiomyocyte autophagy function comprises a bottom plate 1, the upper end of the bottom plate 1 is slidably connected with a mounting plate 2, the upper end of the mounting plate 2 is provided with a plurality of positioning grooves 21, and the positioning grooves 21 are used to position the culture dish; the upper end of the bottom plate 1 is provided with a shaking mechanism 4, and the shaking mechanism 4 is used to drive the mounting plate 2 to slide back and forth, so that the culture dish in the positioning groove 21 shakes to achieve dynamic culture;

[0030] The shaking mechanism 4 includes a first driving device 41 fixed to the upper end of the base plate 1, a first connecting rod 42 is fixed on the driving shaft of the first driving device 41, one end of the first connecting rod 42 is rotatably connected to the second connecting rod 43, a connecting seat 22 is provided on one side of the mounting plate 2, one end of the second connecting rod 43 is hinged to the connecting seat 22, and by controlling the operation of the first driving device 41, the mounting plate 2 can be made to slide back and forth on the upper end of the base plate 1, thereby realizing the dynamic culture of cardiomyocytes.

[0031] A fixing mechanism 3 is provided at the upper end of the mounting plate 2, and the fixing mechanism 3 is used to fix the culture dish on the mounting plate 2, so as to prevent the culture dish in the positioning groove 21 from being thrown out when the mounting plate 2 slides back and forth. At the same time, the fixing mechanism 3 can cover the upper end of the culture dish, so as to prevent the culture fluid and myocardial cells inside the culture dish from leaking when the mounting plate 2 slides back and forth.

[0032] like Figure 4 As shown, the fixing mechanism 3 includes a fixing frame 31 fixed to the upper end of the mounting plate 2, a first rack 32 is slidably connected to the fixing frame 31, a pressing plate 33 is fixed to the lower end of the first rack 32, and the pressing plate 33 can be lowered by controlling the first rack 32 to descend, so as to press the culture dish into the positioning groove 21, and at the same time, the pressing plate 33 can cover the upper end of the culture dish;

[0033] A second driving device 34 is provided on the fixing frame 31 , a first gear 35 is fixed on the driving shaft of the second driving device 34 , the first gear 35 is meshed with the first rack 32 , and the lifting movement of the pressing plate 33 can be controlled by controlling the operation of the second driving device 34 .

[0034] The upper end of the bottom plate 1 is provided with a locking mechanism 5, which is used to limit the movement of the shaking mechanism 4; Figure 6-7As shown, the locking mechanism 5 includes a first fixing plate 51 fixed to the upper end of the bottom plate 1, a sliding rod 52 is slidably connected to the first fixing plate 51, a clamping plate 521 is provided at one end of the sliding rod 52, a second gear 44 is fixed to the driving shaft of the first driving device 41, and the end of the clamping plate 521 can be engaged with two adjacent teeth of the second gear 44, thereby limiting the operation of the shaking mechanism 4;

[0035] A spring 53 is sleeved on the sliding rod 52 , and two ends of the spring 53 are fixedly connected to the side of the first fixed plate 51 and the side of the clamping plate 521 respectively. Under the elastic force of the spring 53 , the end of the clamping plate 521 can be engaged with two adjacent teeth of the second gear 44 .

[0036] The sliding rod 52 is provided with a second rack 522, the first fixed plate 51 is rotatably connected with a rotating shaft 54, a third gear 55 and a fourth gear 56 are fixed on the rotating shaft 54, and the third gear 55 is meshed with the second rack 522;

[0037] The bottom plate 1 is provided with a trigger mechanism 6, which can control the state of the locking mechanism 5, so as to achieve the purpose of controlling the operation timing of the shaking mechanism 4, so that the shaking mechanism 4 can only operate when the pressing plate 33 presses and fixes the culture dish, thereby avoiding leakage of liquid in the culture dish during dynamic culture;

[0038] like Figure 8-9 As shown, the trigger mechanism 6 includes a support plate 61 and a connecting rod 62. The support plate 61 is fixed to the upper end of the base plate 1. The support plate 61 is provided with a T-shaped slide groove in the vertical direction. One end of the connecting rod 62 cooperates with the T-shaped slide groove and is slidably connected. The sliding direction is the vertical direction, so that only the degree of freedom of the connecting rod 62 in the vertical direction is retained. Figure 5 As shown, a second fixing plate 321 is provided on one side of the first rack 32, and the connecting rod 62 passes through the second fixing plate 321 and is slidably connected to the second fixing plate 321, so that when the first rack 32 moves up and down, it can drive the connecting rod 62 to rise and fall synchronously, and the connecting rod 62 will not interfere with the reciprocating sliding of the mounting plate 2;

[0039] A third rack 63 is provided at one end of the connecting rod 62. When the connecting rod 62 descends along with the first rack 32, the third rack 63 can mesh with the fourth gear 56 and drive the sliding rod 52 to slide in a direction away from the second gear 44. Moreover, when the pressing plate 33 presses the culture dish into the positioning groove 21, the clamping plate 521 just leaves the second gear 44, and the shaking mechanism 4 can operate to realize dynamic culture. Thus, the shaking mechanism 4 can operate only when the pressing plate 33 presses and fixes the culture dish, thereby preventing leakage of liquid in the culture dish, instead of relying solely on controlling the first driving device 41 to control the reciprocating sliding timing of the mounting plate 2.

[0040] The third rack 63 is fixed with a lifting rod 64 at the upper end, and the lifting rod 64 passes through the connecting rod 62 in the vertical direction and is slidably connected with the connecting rod 62. A support frame 65 is fixed to the connecting rod 62, and an adjusting rod 66 is threadedly connected to the support frame 65. The lower end of the adjusting rod 66 is rotatably connected to the lifting rod 64. By twisting the adjusting rod 66, the lifting and lowering movement of the lifting rod 64 can be controlled, thereby realizing the adjustment of the vertical position of the third rack 63 relative to the connecting rod 62. When the third rack 63 is meshed with the fourth gear 56 and drives the clamping plate 521 to just leave the second gear 44, the height position of the pressing plate 33 is changed, so that when the culture dishes of different heights are used to culture myocardial cells, the trigger mechanism 6 can still realize the precise control of the shaking timing, so as to avoid the failure of the pressing plate 33 to press and fix the culture dish and seal it when the culture dish slides, resulting in leakage.

[0041] Working principle:

[0042] The culture dish is installed on the mounting plate 2 by slidingly connecting the mounting plate 2 at the upper end of the base plate 1, and the rocking mechanism 4 is set to drive the reciprocating sliding of the mounting plate 2, so as to realize the rocking of the culture dish and realize the dynamic culture of the cardiomyocytes; the fixing mechanism 3 is set at the upper end of the mounting plate 2 to fix the culture dish, and the upper end of the culture dish is sealed to prevent leakage during rocking; the locking mechanism 5 is set at the upper end of the base plate 1 to limit the operation of the rocking mechanism 4, and the locking timing of the locking mechanism 5 is controlled by setting a trigger mechanism 6, so as to control the sliding timing of the rocking mechanism 4. Only when the pressing plate 33 presses and fixes the culture dish, the rocking mechanism 4 can operate to avoid leakage of liquid in the culture dish, instead of relying solely on controlling the first driving device 41 to control the reciprocating sliding timing of the mounting plate 2.

[0043] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0044] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A culture device for down-regulating dox-induced cardiomyocyte autophagy, comprising a bottom plate (1), characterized in that: The upper end of the bottom plate (1) is slidably connected to a mounting plate (2), and a plurality of positioning grooves (21) are provided on the upper end of the mounting plate (2), wherein culture dishes are mounted in the positioning grooves (21); The upper end of the base plate (1) is provided with a shaking mechanism (4), the shaking mechanism (4) comprising a first driving device (41) fixed to the upper end of the base plate (1), a first connecting rod (42) fixed to the driving shaft of the first driving device (41), one end of the first connecting rod (42) being rotatably connected to a second connecting rod (43), a connecting seat (22) being provided on one side of the mounting plate (2), one end of the second connecting rod (43) being hinged to the connecting seat (22), and by controlling the operation of the first driving device (41), the mounting plate (2) can be made to slide back and forth on the upper end of the base plate (1); The upper end of the mounting plate (2) is provided with a fixing mechanism (3), the fixing mechanism (3) comprising a fixing frame (31) fixed to the upper end of the mounting plate (2), a first rack (32) being slidably connected to the fixing frame (31), a pressing plate (33) being fixed to the lower end of the first rack (32), a second driving device (34) being provided on the fixing frame 31, a first gear (35) being fixed to the driving shaft of the second driving device (34), and the first gear (35) and the first rack (32) being meshed with each other; By controlling the pressing plate (33) to descend, the culture dish can be pressed tightly into the positioning groove (21), and at the same time, the pressing plate (33) can cover the upper end of the culture dish; Furthermore, a locking mechanism (5) is provided at the upper end of the base plate (1), and the locking mechanism (5) is used to limit the movement of the shaking mechanism (4); The locking mechanism (5) comprises a first fixed plate (51) fixed to the upper end of the base plate (1), a sliding rod (52) being slidably connected to the first fixed plate (51), a clamping plate (521) being arranged at one end of the sliding rod (52), a second gear (44) being fixed to the driving shaft of the first driving device (41), a spring (53) being sleeved on the sliding rod (52), two ends of the spring (53) being respectively fixedly connected to the side surface of the first fixed plate (51) and the side surface of the clamping plate (521), and under the elastic force of the spring (53), the end of the clamping plate (521) can be clamped between two adjacent teeth of the second gear (44); Furthermore, the sliding rod (52) is provided with a second rack (522), the first fixed plate (51) is rotatably connected with a rotating shaft (54), a third gear (55) and a fourth gear (56) are fixed on the rotating shaft (54), and the third gear (55) and the second rack (522) are meshed with each other; The base plate (1) is provided with a trigger mechanism (6), which includes a support plate (61) and a connecting rod (62). The support plate (61) is fixed to the upper end of the base plate (1). The support plate (61) is provided with a T-shaped slide groove in the vertical direction. One end of the connecting rod (62) cooperates with the T-shaped slide groove and is slidably connected. The sliding direction is the vertical direction. A second fixed plate (321) is provided on one side of the first rack (32). The connecting rod (62) passes through the second fixed plate (321) and is slidably connected to the second fixed plate (321), so that when the first rack (32) moves up and down, it can drive the connecting rod (62) to move up and down synchronously, and the connecting rod (62) will not interfere with the reciprocating sliding of the mounting plate (2); A third rack (63) is provided at one end of the connecting rod (62); when the connecting rod (62) descends along with the first rack (32), the third rack (63) can mesh with the fourth gear (56) and drive the sliding rod (52) to slide in a direction away from the second gear (44); Furthermore, when the pressing plate (33) presses the culture dish into the positioning groove (21), the clamping plate (521) just leaves the second gear (44).

2. The culture device for down-regulating dox-induced cardiomyocyte autophagy according to claim 1, characterized in that: A lifting rod (64) is fixed to the upper end of the third rack (63), the lifting rod (64) passes through the connecting rod (62) in the vertical direction and is slidably connected to the connecting rod (62), a support frame (65) is fixed to the connecting rod (62), an adjusting rod (66) is threadedly connected to the support frame (65), and the lower end of the adjusting rod (66) is rotatably connected to the lifting rod (64), and by twisting the adjusting rod (66), the lifting and lowering movement of the lifting rod (64) can be controlled, thereby realizing the adjustment of the position of the third rack (63) relative to the connecting rod (62) in the vertical direction.