A microbial direct coating spotting instrument

Abstract

The present invention discloses a microbial direct coating spotting instrument, which relates to the technical field of life science instruments, including a bottom plate, a bracket fixedly arranged on the bottom plate, an X module fixedly arranged on one side of the top of the bracket, a vertical mounting plate slidably arranged on the X module, a camera and a colony direct coating module are arranged on the vertical mounting plate, and the colony direct coating module includes a direct coating device, which can move vertically on the vertical mounting plate, a Y module fixedly arranged on the bottom plate, a horizontal mounting plate slidably arranged on the Y module, and a culture dish platform for placing a culture dish and a culture dish cover platform for placing a culture dish cover are fixedly arranged on the horizontal mounting plate. The present invention can accurately measure the colony height, realize the purpose of automatically selecting colonies and selecting or sticking trace bacteria to a target plate.

Description

Technical Field

[0001] The invention relates to the technical field of life science instruments, in particular to a microbial direct coating spotting instrument. Background Art

[0002] A mass spectrometer is a scientific instrument that uses electromagnetic principles to separate ions based on their mass-to-charge ratios, and then measures the mass and content of a substance. Matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOFMS) is a branch of mass spectrometer that can quickly and accurately analyze and identify multiple samples at the same time. Usually, the sample is first fixed on the target point on the target plate, and then sent to the matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOFMS) for analysis and identification.

[0003] The use of MALDI-TOF MS fingerprinting to identify unknown microorganisms is currently the most mature and widely used application of this technology. In the mass range of 2000 to 20000 Da, the microbial mass spectrum is less affected by the culture medium and metabolites, and is insensitive to differential expression during the growth stage of the microorganism, but is very stable for high-abundance protein expression (more conservative protein expression during microbial evolution), has good repeatability, and is specific at the genus and species levels, so it is called peptide mass fingerprinting (PMF). Currently, this technology can be used for species-level microbial identification. Therefore, through specific statistical requirements and standards, multiple mass spectra of strains of known genera and species can be collected, and a standard spectrum can be formed through statistical algorithms to establish a standard bacterial library. When identifying unknown strains, the identification can be completed by comparing the obtained mass spectrum with the fingerprint spectrum in the standard bacterial library through specific scoring rules. Fingerprints have good specificity, so thousands or even tens of thousands of bacterial species can be identified. At present, MALDI-TOFMS has been widely used in the classification and identification of microorganisms such as bacteria, fungi, mycobacteria and Nocardia.

[0004] In order to ensure that matrix-assisted laser desorption ionization-time-of-flight mass spectrometry obtains protein spectrum signals of microorganisms, the classic approach is to extract the proteins in the microorganisms (called the "extraction method"). The extraction process is cumbersome. In order to shorten the identification time, in actual applications, it is often used to directly pick trace bacteria from the culture dish to the target plate, and then drip the matrix on it (called the "direct coating method"). The "extraction method" deals with liquids, and the spotting instruments on the market can basically meet the requirements. However, the "direct coating method" is very difficult. Because the bacterial colonies growing on the culture dish are very irregular and soft, it is very difficult to automatically select colonies and pick trace bacteria to the target plate. Summary of the invention

[0005] The purpose of the present invention is to provide a microbial direct coating spotter to solve the problems existing in the above-mentioned prior art, and to accurately measure the colony height, so as to achieve the purpose of automatically selecting colonies and selecting or sticking trace bacteria to a target plate.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The invention provides a microorganism direct coating spotting instrument, comprising a bottom plate, a bracket fixedly arranged on the bottom plate, an X module fixedly arranged on one side of the top of the bracket, a vertical mounting plate slidably arranged on the X module, a camera and a colony direct coating module arranged on the vertical mounting plate, the camera can adopt a frontal illumination or a backlight illumination mode, so as to select a suitable shooting mode according to needs, improve contrast and positioning effects, the colony direct coating module comprises a direct coating device, the direct coating device can move vertically on the vertical mounting plate, a Y module fixedly arranged on the bottom plate, a horizontal mounting plate slidably arranged on the Y module, a culture dish platform for placing a culture dish and a culture dish cover platform for placing a culture dish cover are fixedly arranged on the horizontal mounting plate; the automatic selection of the colony in the culture dish can be realized by the machine vision function of the camera; the lighting mode of the camera adopts a combination of top lighting and backlight illumination, the top lighting mode can capture a color image of the colony, the backlight illumination mode can capture a perspective image, it is convenient to illuminate and select a transparent culture medium, and the colony position can be accurately determined.

[0008] Optionally, the direct coating device includes a Z1 motor fixedly mounted on a U-shaped frame, the bottom of the Z1 motor is connected to a driving gear, the driving gear is meshed with a driven gear, the driven gear is fixedly sleeved on the outer wall of the direct coating head sleeve, and the direct coating head sleeve is slidably inserted on the U-shaped frame; a transition sleeve is fixedly connected to the bottom of the direct coating head sleeve, a probe is movably arranged in the transition sleeve, and the probe includes a conductive screw, and the conductive screw is fixedly connected to an insulating head, a grounding electrode and a plug in sequence; the bottom of the plug can pass through the transition sleeve and then be fixed to the direct coating head The top of the conductive screw is connected to the electric connector at the top of the sleeve through a flexible cable, the bottom of the grounding electrode can abut against the bottom of the transition sleeve, and the electric connector is electrically connected to the driver at the top of the bracket. After the straight coating head contacts the colony, it can push the probe in the transition sleeve in the reverse direction, so that the colony height can be determined by the conductive connection between the probe and the electric connector; one side of the U-shaped frame is slidably set on the vertical mounting plate, and the top of the U-shaped frame is connected to the Z2 motor fixedly set above the vertical mounting plate through a screw nut.

[0009] Optionally, a syringe is also included, the top of the piston rod of the syringe is fixedly connected with a nut via a connecting block, the nut is threadedly sleeved on the screw, the screw is transmission connected to the Z3 motor, and the Z3 motor is fixedly arranged above the syringe mounting plate; the syringe mounting plate is slidably arranged on the vertical mounting plate, and the top of the syringe mounting plate is transmission connected to the Z4 motor fixedly arranged above the vertical mounting plate via a screw nut; the camera is fixedly arranged between the syringe and the colony direct coating module.

[0010] Optionally, it also includes a target plate drying module, which includes a guide rail fixedly arranged on the base plate, a target holding platform slidably arranged on the guide rail, a target holder is fixedly installed on the top of the target holding platform, an O-ring is fixedly arranged on the outside of the target holder, the target plate is placed in the target holder, and the target plate is placed in the O-ring; drying brackets are symmetrically arranged on both sides of the guide rail, a drying cover plate is slidably sleeved above the drying bracket, an exhaust joint is installed on the drying cover plate, the bottom of the exhaust joint is located below the drying cover plate, and the top of the exhaust joint passes through the drying cover plate and is externally connected to a vacuum pump through a vacuum pipeline.

[0011] Optionally, a limit block is fixedly provided on the top of the drying bracket, and a compression spring is sleeved on the drying bracket. The compression spring is located between the limit block and the drying cover plate, and the compression spring can fix the drying cover plate and the target holder in contact.

[0012] Optionally, the target support platform is connected to a horizontal motor through a horizontally arranged lead screw nut, and the horizontal motor is electrically connected to a micro switch located at one side of the target support platform.

[0013] Optionally, the X module includes a first horizontal slide rail fixedly arranged on one side of the top of the bracket, a first transmission chain is arranged on the first horizontal slide rail, the first transmission chain is transmission-connected to an X motor fixedly arranged on one side of the top of the bracket, the vertical mounting plate is slidably arranged on the first horizontal slide rail, and the vertical mounting plate is fixedly connected to the outer side of the first transmission chain through a connecting device; a photoelectric switch is arranged above the first horizontal slide rail.

[0014] Optionally, the Y module includes a second horizontal slide rail fixedly set on the base plate, a second transmission chain is arranged on the second horizontal slide rail, the second transmission chain is transmission-connected to the Y motor fixedly set on the base plate, the horizontal mounting plate is slidably set on the second horizontal slide rail, and the horizontal mounting plate is fixedly connected to the outer side of the second transmission chain through a connecting device; a photoelectric switch is arranged on one side of the second horizontal slide rail.

[0015] Optionally, a direct coating head storage box and a waste box are fixedly installed on the horizontal mounting plate, and the direct coating head storage box and the waste box are located on one side of the culture dish platform and the culture dish cover platform. An unloader is arranged above the waste box, and the unloader includes a horizontally protruding fixed plate, and a U-shaped groove is opened on the outer side of the fixed plate.

[0016] Optionally, a protective frame is fixedly provided at the outer edge of the bottom plate, and a protective plate is installed on the protective frame; a reagent tank is fixedly provided on the bottom plate, and a plurality of centrifuge tubes are placed on the reagent tank, and the centrifuge tubes are respectively filled with matrix solution and different lysis solutions.

[0017] Compared with the prior art, the present invention has achieved the following technical effects:

[0018] The present invention is suitable as a supporting device for microbial identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The present invention has diversified functions and can realize the purpose of automatically selecting bacterial colonies and picking trace bacteria to target plates. In addition, the sample after applying the matrix can be vacuum dried, which shortens the drying time, greatly improves the detection efficiency, and has uniform crystallization. The camera can realize the function of barcode reading and automatic selection of bacterial colonies using machine vision; the syringe can complete the application of the matrix; the automatic direct coating device has the functions of automatically loading the direct coating head, unloading the direct coating head, and automatically directly coating bacteria selection and target coating. The control device drives the device to move downward as a whole, and the plug can be fixedly inserted into the conical hole of the direct coating head. An unloader is provided above the waste box, and the direct coating head is clamped in the groove of the unloader above the waste box. The device is moved upward, and the plug can be quickly separated from the direct coating head, so that the direct coating head can be quickly and automatically loaded and unloaded. The motor drives the direct coating head to rotate quickly, so that the bottom of the direct coating head rotates on the surface of the bacterial colony or the target plate, and automatic direct coating bacteria selection and target coating are realized. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 This is a schematic diagram of the overall structure of a microbial direct coating spotting instrument according to Embodiment 1 of the present invention;

[0021] Figure 2 This is a schematic diagram of the internal structure of a microbial direct coating spotting instrument according to Embodiment 1 of the present invention;

[0022] Figure 3 It is a schematic side view of the internal structure of the microbial direct coating spotting instrument according to the first embodiment of the present invention;

[0023] Figure 4 This is a schematic diagram of the structure of an unloader of a microbial direct coating spotting instrument according to a first embodiment of the present invention;

[0024] Figure 5 This is a schematic diagram of the structure of a target plate drying module of a microbial direct coating spotting instrument according to a first embodiment of the present invention;

[0025] Figure 6 This is a schematic diagram of the main view of a target plate drying module of a microbial direct coating spotting instrument according to a first embodiment of the present invention;

[0026] Figure 7 This is a schematic diagram of the main view of the target plate drying module of the microbial direct coating spotting instrument according to the second embodiment of the present invention;

[0027] Description of the accompanying drawings: 1-direct coating device; 2-culture dish platform; 3-camera module; 4, colony direct coating module; 5, target plate drying module; 6-bottom plate; 7-culture dish platform; 8-culture dish; 9-culture dish cover; 10-culture dish cover platform; 11-Y module; 12-direct coating head sleeve; 13-Z1 motor; 14-syringe; 15-camera; 16-Z2 motor; 17-Z3 motor; 18-Z4 motor; 19-X module; 20-X motor; 21-photoelectric switch; 22-direct coating head storage box; 2 3-waste box; 24-guide rail; 25-target support platform; 26-target support; 27-drying cover; 28-micro switch; 29-bracket; 30-driver; 31-vacuum pump; 32-exhaust connector; 33-drying bracket; 34-compression spring; 35-protection frame; 36-protection plate; 37-unloader; 38-horizontal motor; 39-O-ring; 40-driving gear; 41-driven gear; 42-U-shaped frame; 43-straight coating head; 44-plug; 45-reagent tank; 46-centrifuge tube; 47-backlight source. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The purpose of the present invention is to provide a microbial direct coating spotter to solve the problems existing in the above-mentioned prior art, and to accurately measure the colony height, so as to achieve the purpose of automatically selecting colonies and selecting or sticking trace bacteria to a target plate.

[0030] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Embodiment 1

[0032] The present invention provides a microbial direct coating spotting instrument, such as Figure 1 , Figure 2 and Figure 3 As shown, it includes a bottom plate 6, a protective frame 35 is fixedly provided at the outer edge position of the bottom plate 6, a protective plate 36 is installed on the protective frame 35, a bracket 29 is fixedly provided on the bottom plate 6, an X module 19 is fixedly provided on the top side of the bracket 29, a vertical mounting plate is slidably provided on the X module 19, a camera module 3 and a colony direct coating module 4 are provided on the vertical mounting plate, the colony direct coating module 4 includes a direct coating device 1, the direct coating device 1 can move vertically on the vertical mounting plate, a Y module 11 is fixedly provided on the bottom plate 6, a horizontal mounting plate is slidably provided on the Y module 11, a culture dish platform 7 for placing a culture dish 8 and a culture dish cover platform 10 for placing a culture dish cover 9 are fixedly provided on the horizontal mounting plate; thereby, the X module 19 and the Y module 11 can realize the relative movement of the position of the culture dish 8 and the direct coating device 1, and the direct coating device 1 can move up and down, thereby realizing the final contact between the direct coating device 1 and the colony in the culture dish 8. The present invention also includes a syringe 14 and a target plate drying module 5. The top of the piston rod of the syringe 14 is fixedly connected with a nut through a connecting block. The nut is threadedly sleeved on the screw rod. The screw rod is connected to the Z3 motor 17 in transmission. The Z3 motor 17 is fixedly arranged above the syringe mounting plate; the syringe mounting plate is slidably arranged on the vertical mounting plate. The top of the syringe mounting plate is connected to the Z4 motor 18 fixedly arranged above the vertical mounting plate through a screw nut. The Z4 motor 18 can realize the up and down movement of the syringe 14. The Z3 motor 17 can drive the piston rod of the syringe 14 to move up and down, thereby achieving the effect of the syringe 14 adsorbing or injecting the matrix. A reagent tank 45 is arranged on the bottom plate 6 at a position outside the target support 25. Three 1.5mL centrifuge tubes 46 can be placed on the reagent tank 45, which are respectively filled with matrix liquid and different lysate. The syringe 14 can be controlled to move to the position of the reagent tank 45 by the X module, and different reagents can be selected for application; the camera module 3 is fixedly arranged between the syringe 14 and the colony direct coating module 4, which includes a camera 15. As Figure 5 and Figure 6As shown, the target plate drying module 5 includes a guide rail 24 fixedly arranged on the bottom plate 6, a target support platform 25 is slidably arranged on the guide rail 24, a target support 26 is fixedly installed on the top of the target support platform 25, the target support 26 is used to place the target plate, an O-ring 39 is arranged on the outside of the target support 26, the target plate is placed in the O-ring 39, the top of the O-ring 39 can be sealed and connected with the bottom surface of the drying cover 27, so as to ensure that the target plate is always sealed during the drying process, and the target support moves to the bottom of the drying cover when drying is required, and the drying cover is supported by four compression springs to keep it pressed against the sealing O-ring on the target support. The vacuum pump then performs vacuum drying through the vacuum joint 49; the guide rail 24 is symmetrically arranged with a drying bracket 33 on both sides, the drying bracket 33 is slidably sleeved with a drying cover 27, the drying cover 27 is installed with a vacuum joint 32, the bottom of the vacuum joint 32 is located below the drying cover 27, and the top of the vacuum joint 32 passes through the drying cover 27 and is connected to the vacuum pump 31 through a vacuum pipeline. A limit block is fixedly arranged on the top of the drying bracket 33, and a compression spring 34 is sleeved on the drying bracket 33. The compression spring 34 is located between the limit block and the drying cover plate 27. The compression spring 34 is in a compressed state, so that its elastic force can fix the drying cover plate 27 and the target holder 26 in contact. The target holder platform 25 is connected to a horizontal motor 38 through a horizontally arranged screw nut, and the horizontal motor 38 is electrically connected to a micro switch 28 located on one side of the target holder platform 25; during drying, the target holder platform 25 and the target holder 26 move to the bottom of the drying cover plate 27, and are compressed by the compression spring 34, and the vacuum pump 31 is connected to the exhaust joint 32 through a pipeline to achieve vacuum drying.

[0033] The direct coating device 1 includes a Z1 motor 13 fixedly arranged on a U-shaped frame 42, a driving gear 40 is connected to the bottom of the Z1 motor 13, the driving gear 40 is meshed with a driven gear 41, the driven gear 41 is fixedly sleeved on the outer wall of the direct coating head sleeve 12, and the direct coating head sleeve 12 is slidably inserted on the U-shaped frame 42; a transition sleeve is fixedly connected to the bottom of the direct coating head sleeve 12, a probe is movably arranged in the transition sleeve, the bottom of the probe can pass through the transition sleeve and be fixedly sleeved with the direct coating head 43, and the top of the probe can be fixedly sleeved with the direct coating head The bottom of the sleeve is fixedly connected; the top of the probe is connected to the electrical connector located at the top of the straight coating head sleeve 12 through a flexible cable, and the electrical connector is electrically connected to the driver 30 located at the top of the bracket 29; one side of the U-shaped frame 42 is slidably set on the vertical mounting plate, and the top of the U-shaped frame 42 is connected to the Z2 motor 16 fixedly set above the vertical mounting plate through a screw nut. The screw nut of the present invention is an existing transmission device, and can also be a screw nut, which can ultimately convert the rotary motion of the motor into the horizontal or vertical motion of the connected equipment. A conical hole with a gradually increasing diameter from bottom to top is provided at the top of the straight coating head 43, and the bottom of the probe can be fixedly sleeved in the conical hole after passing through the transition sleeve. The probe includes a conductive screw, and the conductive screw is fixedly connected with an insulating head, a grounding electrode and a plug 44 in sequence; the bottom of the plug 44 can be fixedly sleeved with the straight coating head 43 after passing through the transition sleeve, and the top of the conductive screw is connected to the electrical connector located at the top of the sleeve through a flexible cable, and the bottom of the grounding electrode can abut against the bottom of the transition sleeve. A limiting groove is provided on the top of the insulating head, and a limiting protrusion is provided at the bottom of the sleeve, and the limiting protrusion can be inserted into the limiting groove. The bottom of the direct coating head sleeve 12 is fixedly sleeved inside the transition sleeve. Before direct coating of the colony, the probe hangs freely, and the grounding electrode abuts against the bottom of the transition sleeve made of metal material, that is, at this time, the electrical connector outputs a grounding signal. When the direct coating head 43 contacts the colony, the probe is pushed upward in the reverse direction. At this time, the grounding electrode is separated from the bottom of the transition sleeve made of metal material, and the electrical connector is turned on, so that the position and height of the colony can be judged and measured by the conduction between the probe and the electrical connector. In different embodiments, a micro-pressure sensor can also be installed at the probe. When the direct coating head 43 contacts the colony, the pressure generated by it is fed back to the micro-pressure sensor of the probe, and then the position and height of the colony are judged according to the signal of the micro-pressure sensor. When direct coating is required to select bacterial colonies, the camera 15 of the camera module 3 takes an image of the bacterial colonies, and the growth position of the bacterial colonies is determined by machine vision; then the screw and nut of the X module 19 and the Z2 motor 16 drive the direct coating device 1 of the present invention close to the bacterial colonies in the culture dish 8. When the direct coating head touches the bacterial colonies, the direct coating head will move upward due to squeezing, and then the probe will move upward, and the grounding electrode will be separated from the transition sleeve at this time, so that the electrical connector outputs a circuit-breaking signal to the control terminal. At this time, the control device stops working, and the motor starts to rotate, driving the direct coating head to rotate through the driving gear and the driven gear, so that the bacterial colonies are attached to the head of the direct coating head, completing the process of direct coating and picking bacteria.The working process of direct coating of bacteria to the target plate is similar to the above process. A direct coating head storage box 22 and a waste box 23 are fixedly arranged on the horizontal mounting plate, and an unloader 37 is arranged above the waste box, such as. Figure 4 As shown, the unloader 37 includes a horizontally convex fixing plate, and a U-shaped groove is provided on the outer side of the fixing plate. The width of the U-shaped groove is larger than the diameter of the plug and smaller than the size of the maximum diameter of the tapered hole, which is convenient for unloading the direct coating head, because the plug and the direct coating head are sheathed together by the tapered socket, and there is a 2-3mm empty section between the two. When the direct coating head needs to be unloaded, the U-shaped groove of the unloader is aligned with the empty section between the plug and the direct coating head, and the Z2 motor drives the direct coating mechanism to move upward again, and the direct coating head will be detached from the plug. The unloading method is not specifically limited here. In different embodiments, a horizontal outer edge is provided on the inner side of the top of the waste box 23, which is fixedly connected with the top edge of the direct coating head when unloading the direct coating head. The direct coating head storage box 22 and the waste box 23 are located on one side of the culture dish platform 7 and the culture dish cover platform 10. The control device drives the equipment to move downward as a whole, so that the plug can be fixedly inserted into the conical hole of the direct coating head, and the direct coating head can be connected to the waste box 23. The equipment can be moved upward to quickly separate the plug from the direct coating head, so that the direct coating head can be quickly and automatically loaded and unloaded, which is convenient for the direct coating device to quickly install or unload the direct coating head.

[0034] Further preferably, the X module 19 includes a first horizontal slide rail fixedly arranged on one side of the top of the bracket 29, a first transmission chain is arranged on the first horizontal slide rail, the first transmission chain is connected to the X motor 20 fixed to one side of the top of the bracket, a vertical mounting plate is slidably arranged on the first horizontal slide rail, and the vertical mounting plate is fixedly connected to the outer side of the first transmission chain by a connecting device; a photoelectric switch 21 is arranged above the first horizontal slide rail. The Y module 11 includes a second horizontal slide rail fixedly arranged on the bottom plate, a second transmission chain is arranged on the second horizontal slide rail, the second transmission chain is connected to the Y motor fixed to the bottom plate, a horizontal mounting plate is slidably arranged on the second horizontal slide rail, and the horizontal mounting plate is fixedly connected to the outer side of the second transmission chain by a connecting device; a photoelectric switch 21 is arranged on one side of the second horizontal slide rail.

[0035] When the device of the present invention is working, after the sample is injected, the camera module 3 shoots the barcode on the culture dish cover 9, the direct coating device 1 automatically installs the direct coating gun head, the direct coating device 1 picks up the colonies, and the direct coating device 1 directly coats the colonies on the target plate. The matrix applying module, that is, the syringe 14 applies the matrix, unloads the direct coating device gun head, stops when the set number of colonies is reached or the stop button is pressed, and the vacuum drying module vacuum dries the target plate.

[0036] Embodiment 2

[0037] This embodiment is a further improvement made on the basis of the first embodiment. In this embodiment, in addition to the front-lighting mode adopted by the camera module 3 of the first embodiment, a backlighting mode is also added. Figure 7 As shown, in this embodiment, a backlight source 47 is provided at the culture dish platform 7, so that the backlight source 47 is utilized to realize backlight shooting of the camera 15, and the backlight shooting method is adopted for the light-colored transparent culture medium, so that the contrast of the colony is higher and the positioning effect is more accurate.

[0038] In the description of the present invention, it should be noted that the terms "center", "top", "bottom", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0039] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A microbial direct coating spotting instrument, characterized in that: It comprises a bottom plate, a bracket is fixedly arranged on the bottom plate, an X module is fixedly arranged on one side of the top of the bracket, a vertical mounting plate is slidably arranged on the X module, a camera and a colony direct coating module are arranged on the vertical mounting plate, the colony direct coating module comprises a direct coating device, and the direct coating device can move vertically on the vertical mounting plate, a Y module is fixedly arranged on the bottom plate, a horizontal mounting plate is slidably arranged on the Y module, and a culture dish platform for placing a culture dish and a culture dish cover platform for placing a culture dish cover are fixedly arranged on the horizontal mounting plate; The machine vision function of the camera can realize automatic selection of colonies in the culture dish; the lighting method of the camera adopts a combination of top lighting and backlighting; the direct coating device includes a Z1 motor fixedly arranged on a U-shaped frame, the bottom of the Z1 motor is connected to a driving gear, the driving gear is meshed with a driven gear, the driven gear is fixedly sleeved on the outer wall of the direct coating head sleeve, and the direct coating head sleeve is slidably inserted on the U-shaped frame; a transition sleeve is fixedly connected to the bottom of the direct coating head sleeve, a probe is movably arranged in the transition sleeve, the probe includes a conductive screw, and the conductive screw is fixedly connected with an insulating head, a grounding electrode and a plug in sequence; the bottom of the plug can pass through the transition sleeve and be fixedly sleeved with the direct coating head, the top of the conductive screw is connected to the electrical connector located at the top of the sleeve through a flexible cable, and the bottom of the grounding electrode can abut against the bottom of the transition sleeve. , the electrical connector is electrically connected to the driver located at the top of the bracket, and the direct coating head can push the probe in the transition sleeve in the reverse direction after contacting the colony, so that the colony height can be measured by relying on the conductive connection between the probe and the electrical connector; one side of the U-shaped frame is slidably arranged on the vertical mounting plate, and the top of the U-shaped frame is connected to the Z2 motor fixedly arranged above the vertical mounting plate through a screw nut; it also includes a syringe, the top of the piston rod of the syringe is fixedly connected with a nut through a connecting block, the nut is threadedly sleeved on the screw, the screw is transmission-connected to the Z3 motor, and the Z3 motor is fixedly arranged above the syringe mounting plate; the syringe mounting plate is slidably arranged on the vertical mounting plate, and the top of the syringe mounting plate is transmission-connected to the Z4 motor fixedly arranged above the vertical mounting plate through a screw nut; the camera is fixedly arranged between the syringe and the colony direct coating module.

2. The microbial direct coating spotting instrument according to claim 1, characterized in that: It also includes a target plate drying module, which includes a guide rail fixedly arranged on the bottom plate, a target support platform slidably arranged on the guide rail, a target support is fixedly installed on the top of the target support platform, an O-ring is fixedly arranged on the outside of the target support, the target support is used to place the target plate, and the target plate is placed in the O-ring; drying brackets are symmetrically arranged on both sides of the guide rail, a drying cover plate is slidably sleeved above the drying bracket, an exhaust joint is installed on the drying cover plate, the bottom of the exhaust joint is located below the drying cover plate, and the top of the exhaust joint passes through the drying cover plate and is externally connected to a vacuum pump through a vacuum pipeline.

3. The microbial direct coating spotting instrument according to claim 2, characterized in that: A limit block is fixedly arranged on the top of the drying bracket, and a compression spring is sleeved on the drying bracket. The compression spring is located between the limit block and the drying cover plate, and the compression spring can fix the drying cover plate and the target holder in contact.

4. The microbial direct coating spotting instrument according to claim 2, characterized in that: The target support platform is connected to a horizontal motor through a horizontally arranged lead screw nut, and the horizontal motor is electrically connected to a micro switch located at one side of the target support platform.

5. The microbial direct coating spotting instrument according to claim 1, characterized in that: The X module includes a first horizontal slide rail fixedly arranged on one side of the top of the bracket, a first transmission chain is arranged on the first horizontal slide rail, the first transmission chain is transmission-connected to the X motor fixedly arranged on one side of the top of the bracket, the vertical mounting plate is slidably arranged on the first horizontal slide rail, and the vertical mounting plate is fixedly connected to the outer side of the first transmission chain through a connecting device; a photoelectric switch is arranged above the first horizontal slide rail.

6. The microbial direct coating spotting instrument according to claim 1, characterized in that: The Y module includes a second horizontal slide rail fixedly arranged on the base plate, a second transmission chain is arranged on the second horizontal slide rail, the second transmission chain is transmission-connected to the Y motor fixedly arranged on the base plate, the horizontal mounting plate is slidingly arranged on the second horizontal slide rail, and the horizontal mounting plate is fixedly connected to the outer side of the second transmission chain through a connecting device; a photoelectric switch is arranged on one side of the second horizontal slide rail.

7. The microbial direct coating spotting instrument according to claim 1, characterized in that: A direct coating head storage box and a waste box are fixedly arranged on the horizontal mounting plate, and the direct coating head storage box and the waste box are located on one side of the culture dish platform and the culture dish cover platform. An unloader is arranged above the waste box, and the unloader includes a horizontally protruding fixed plate, and a U-shaped groove is opened on the outer side of the fixed plate.

8. The microbial direct coating spotting instrument according to claim 1, characterized in that: A protection frame is fixedly arranged at the outer edge of the bottom plate, and a protection plate is installed on the protection frame; a reagent tank is fixedly arranged on the bottom plate, and a plurality of centrifuge tubes are placed on the reagent tank, and the centrifuge tubes are respectively filled with matrix solution and lysate.

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