Water cooling device suitable for PCR instrument

Abstract

The present application relates to PCR instrument technical field, specifically to a kind of water cooling device suitable for PCR instrument, including: pedestal, installation groove is equipped on it, the installation groove is used to place hole plate, the installation groove is equipped with annular groove at slot opening, the hole plate is equipped with plug-in board, the plug-in board is inserted into the annular groove;Cooling box, water injection pipe and return pipe are connected on it, water pump is connected on the water injection pipe, the water injection pipe and the return pipe are all communicated with the installation groove, the connection position of the return pipe and the pedestal is higher than the connection position of the water injection pipe and the pedestal, the hole plate bottom can be immersed in cooling water;Partition, in the cooling box and the internal space of the cooling box is divided into upper cavity and lower cavity, the return pipe is connected with the lateral wall of the upper cavity, the water injection pipe is connected with the lateral wall of the lower cavity, the partition is equipped with a plurality of leakage holes.The present application wants to solve the low defect of heat dissipation efficiency in prior art.

Description

Technical Field

[0001] This invention relates to the field of PCR instrument technology, and more specifically to a water-cooling device suitable for PCR instruments. Background Technology

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. PCR utilizes the fact that DNA denatures into single strands at 95°C in vitro. At a lower temperature (usually around 60°C), primers bind to the single strands according to the principle of complementary base pairing. The temperature is then adjusted to the optimal reaction temperature of DNA polymerase (around 72°C), where the DNA polymerase synthesizes the complementary strand along the phosphate-to-pentose direction. The execution of PCR generally requires precise control of the temperature and heating time at each stage of the reaction using a PCR instrument.

[0003] Currently, the well plates used to hold test tubes in PCR instruments in related technologies usually use air cooling, which has the drawback of low heat dissipation efficiency. Summary of the Invention

[0004] Therefore, the present invention aims to solve the problem of low heat dissipation efficiency in the prior art, thereby providing a water-cooling device suitable for PCR instruments.

[0005] A water-cooling device suitable for a PCR instrument includes: a base with a mounting groove for placing a well plate; an annular groove at the opening of the mounting groove; an insert plate on the well plate that is inserted into the annular groove; a cooling box with an injection pipe and a return pipe connected thereto; a water pump connected to the injection pipe; both the injection pipe and the return pipe are connected to the mounting groove; the return pipe is connected to the base at a higher position than the injection pipe is connected to the base; and the bottom of the well plate can be immersed in cooling water; a partition located inside the cooling box and dividing the internal space of the cooling box into an upper chamber and a lower chamber; the return pipe is connected to the side wall of the upper chamber; the injection pipe is connected to the side wall of the lower chamber; the partition has several leakage holes; and a feed inlet is located at the top of the cooling box for injecting crushed ice into the upper chamber.

[0006] Optionally, a first cooling plate is provided on one side wall of the lower cavity in a vertical direction, and a second cooling plate is provided on the other side wall in a vertical direction. The first cooling plate and the second cooling plate are spaced apart, and cooling water can flow through the first cooling plate and the second cooling plate in sequence to the bottom of the cooling tank.

[0007] Optionally, both the first cooling plate and the second cooling plate are provided with a plurality of water guide grooves.

[0008] Optionally, a sealing gasket is provided inside the annular groove.

[0009] Optionally, a rotating rod is provided in the lower cavity, one end of which extends out of the cooling box and is connected to a drive motor. The drive motor is used to drive the rotating rod to rotate around its own axis, and several stirring blades are connected to the side wall of the rotating rod.

[0010] Optionally, the stirring plate is provided with a water storage box, which is used to scoop up water from the cooling tank.

[0011] Optionally, the lower cavity sidewall is provided with an air duct, and a fan is provided inside the air duct. The fan is used to blow air into the water in the lower cavity. A louver is provided at the opening of the air duct, and the louver is used to adjust the air direction.

[0012] Optionally, the louver is provided with a rotating shaft, one end of which extends out of the air duct and is connected to a gear. A spur rack is slidably connected to the side wall of the air duct, and the spur rack meshes with the gear.

[0013] Optionally, the side wall of the duct is provided with a sliding groove, and the rack is provided with a sliding plate, the sliding plate slidingly engaging with the sliding groove.

[0014] The technical solution of this invention has the following advantages:

[0015] 1. This invention provides a water-cooling device suitable for a PCR instrument, comprising: a base with a mounting groove for placing a well plate, an annular groove at the opening of the mounting groove, an insert plate on the well plate, the insert plate being inserted into the annular groove; a cooling box connected to a water inlet pipe and a water return pipe, a water pump connected to the water inlet pipe, both the water inlet pipe and the water return pipe communicating with the mounting groove, the connection point of the water return pipe to the base being higher than the connection point of the water inlet pipe to the base, and the bottom of the well plate being immersable in cooling water; a partition located inside the cooling box and dividing the internal space of the cooling box into an upper chamber and a lower chamber, the water return pipe being connected to the side wall of the upper chamber, the water inlet pipe being connected to the side wall of the lower chamber, the partition having a plurality of leakage holes, and a material inlet at the top of the cooling box for injecting crushed ice into the upper chamber. With the above setup, when the operator uses the PCR instrument, the plate is inserted into the annular groove, so that the plate is placed at the opening of the mounting slot. Then, crushed ice is added to the upper chamber through the feed port. When the PCR instrument is working, the water pump is started, and the water pump injects cooling water from the cooling tank into the mounting slot. The water level in the mounting slot gradually rises, so that the plate is submerged in the cooling water. Then, the cooling water flows back to the upper chamber from the return water pipe, mixes with the crushed ice to cool it down, and flows to the lower chamber through the leak holes on the partition. This cycle is repeated, so that the cooling water can efficiently cool the plate.

[0016] 2. This invention provides a water-cooling device suitable for a PCR instrument. A first cooling plate is vertically arranged on one side wall of the lower chamber, and a second cooling plate is vertically arranged on the other side wall. The first and second cooling plates are spaced apart, allowing cooling water to flow sequentially through both plates to the bottom of the cooling chamber. This arrangement increases the contact area and time between the cooling water and air, further improving the cooling rate and thus enabling more efficient cooling of the well plates.

[0017] 3. This invention provides a water-cooling device suitable for a PCR instrument, wherein both the first cooling plate and the second cooling plate are provided with a plurality of water guiding grooves. Through this arrangement, the water guiding grooves increase the contact area between the first and second cooling plates and the cooling water, thereby improving the cooling rate of the cooling water.

[0018] 4. This invention provides a water-cooling device suitable for PCR instruments, wherein a sealing gasket is provided inside the annular groove. Through this arrangement, the sealing gasket prevents cooling water from leaking out from the opening of the mounting groove.

[0019] 5. This invention provides a water-cooling device suitable for a PCR instrument. A rotating rod is provided within the lower chamber, with one end extending out of the cooling box and connected to a drive motor. The drive motor drives the rotating rod to rotate around its own axis. Several stirring blades are connected to the side wall of the rotating rod. With this configuration, the operator starts the drive motor, which drives the rotating rod to rotate. The stirring blades then lift the cooling water, increasing the contact area between the cooling water and the air, thereby improving the cooling rate of the cooling water.

[0020] 6. This invention provides a water-cooling device suitable for a PCR instrument, wherein the stirring plate is equipped with a water storage box for scooping water from the cooling tank. With this configuration, the water storage box can scoop water from the cooling tank, thereby allowing the stirring plate to dissipate more cooling water and improve working efficiency.

[0021] 7. This invention provides a water-cooling device suitable for a PCR instrument. The lower chamber sidewall is provided with an air duct, and a fan is installed inside the air duct. The fan is used to blow air into the water in the lower chamber. Louvers are provided at the opening of the air duct for adjusting the airflow direction. Through the above arrangement, the fan blows air into the cooling water, increasing the airflow speed around the cooling water, thereby improving the cooling rate of the cooling water.

[0022] 8. This invention provides a water-cooling device suitable for PCR instruments. The louvers are equipped with a rotating shaft, one end of which extends out of the air duct and is connected to a gear. A rack is slidably connected to the side wall of the air duct, and the rack meshes with the gear. With this configuration, the operator can drive several gears to rotate synchronously by sliding the rack up and down, thereby rotating the rotating shaft and then the louvers, thus achieving the purpose of adjusting the airflow direction.

[0023] 9. This invention provides a water-cooling device suitable for a PCR instrument, wherein the side wall of the air duct is provided with a sliding groove, and a sliding plate is provided on the rack, the sliding plate slidingly engaging with the sliding groove. With this configuration, the rack can slide more smoothly on the air duct through the engagement of the sliding plate with the sliding groove. Attached Figure Description

[0024] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the structure of a water-cooling device suitable for a PCR instrument according to an embodiment of this application;

[0026] Figure 2 This is a schematic diagram of the structure of a water cooling tank for a PCR instrument according to an embodiment of this application;

[0027] Figure 3 This is a schematic diagram of the structure of a water storage box for a water-cooling device suitable for a PCR instrument, according to an embodiment of this application.

[0028] Figure 4 This is a schematic diagram of the structure of the louvers of a water-cooling device suitable for a PCR instrument according to an embodiment of this application;

[0029] Figure 5 This is a schematic diagram of the structure of a straight toothed rack for a water-cooling device suitable for a PCR instrument, according to an embodiment of this application.

[0030] Explanation of reference numerals in the attached drawings: 1. Base; 2. Mounting groove; 3. Orifice plate; 4. Annular groove; 5. Insert plate; 6. Water injection pipe; 7. Water return pipe; 8. Water pump; 9. Upper chamber; 10. Lower chamber; 11. Leakage hole; 12. Material inlet; 13. First cooling plate; 14. Second cooling plate; 15. Water guide groove; 16. Sealing gasket; 17. Rotating rod; 18. Drive motor; 19. Stirring blade; 20. Water storage box; 21. Air duct; 22. Fan; 23. Louver; 24. Rotating shaft; 25. Gear; 26. Spur rack; 27. Slide groove; 28. Sliding vane; 29. ​​Cooling box; 30. Partition plate. Detailed Implementation

[0031] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0033] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0034] Furthermore, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0035] Example 1

[0036] Reference Figure 1-5This invention provides a water-cooling device suitable for a PCR instrument, comprising: a base 1 with a mounting groove 2 for placing a well plate 3, an annular groove 4 at the opening of the mounting groove 2, and an insert plate 5 on the well plate 3 inserted into the annular groove 4; a cooling box 29 connected to a water injection pipe 6 and a water return pipe 7, a water pump 8 connected to the water injection pipe 6, both the water injection pipe 6 and the water return pipe 7 communicating with the mounting groove 2, and the water return pipe 7 being connected to the base 1. The connection point is higher than the connection point between the water injection pipe 6 and the base 1, and the bottom of the perforated plate 3 can be immersed in cooling water; the partition plate 30 is located inside the cooling box 29 and divides the internal space of the cooling box 29 into an upper chamber 9 and a lower chamber 10, the return water pipe 7 is connected to the side wall of the upper chamber 9, the water injection pipe 6 is connected to the side wall of the lower chamber 10, the partition plate 30 is provided with a plurality of leakage holes 11, and the top of the cooling box 29 is provided with a material inlet 12, which is used to inject crushed ice into the upper chamber 9.

[0037] With the above setup, when the operator uses the PCR instrument, the insert plate 5 is inserted into the annular groove 4, so that the well plate 3 is placed at the opening of the mounting groove 2. Then, crushed ice is added to the upper chamber 9 through the feed port 12. When the PCR instrument is working, the water pump 8 is started, and the water pump 8 injects the cooling water in the cooling tank 29 into the mounting groove 2. The water level in the mounting groove 2 gradually rises, so that the well plate 3 is submerged in the cooling water. Then, the cooling water flows back to the upper chamber 9 from the return water pipe 7, mixes with the crushed ice to cool down, and flows to the lower chamber 10 through the leakage hole 11 on the partition 30. This cycle is repeated, so that the cooling water can efficiently cool the well plate 3.

[0038] A first cooling plate 13 is vertically arranged on one side wall of the lower cavity 10, and a second cooling plate 14 is vertically arranged on the other side wall. The first cooling plate 13 and the second cooling plate 14 are spaced apart, allowing cooling water to flow sequentially through the first cooling plate 13 and the second cooling plate 14 to the bottom of the cooling tank 29. This arrangement increases the contact area and time between the cooling water and air as it passes through the first cooling plate 13 and the second cooling plate 14, further improving the cooling rate of the cooling water and thus enabling more efficient cooling of the perforated plate 3.

[0039] Both the first cooling plate 13 and the second cooling plate 14 are provided with a plurality of water guiding grooves 15. Through the above arrangement, the water guiding grooves 15 increase the contact area between the first cooling plate 13, the second cooling plate 14 and the cooling water, thereby improving the cooling rate of the cooling water.

[0040] A sealing gasket 16 is provided inside the annular groove 4. With the above arrangement, the sealing gasket 16 can prevent cooling water from leaking out from the groove opening of the mounting groove 2.

[0041] A rotating rod 17 is installed inside the lower chamber 10. One end of the rotating rod 17 extends out of the cooling box 29 and is connected to a drive motor 18. The drive motor 18 drives the rotating rod 17 to rotate around its own axis. Several stirring blades 19 are connected to the side wall of the rotating rod 17. With the above configuration, the operator starts the drive motor 18, which drives the rotating rod 17 to rotate. The stirring blades 19 stir up the cooling water, increasing the contact area between the cooling water and the air, thereby improving the cooling rate of the cooling water.

[0042] The stirring plate 19 is equipped with a water storage box 20, which is used to scoop up water from the cooling tank 29. With the above configuration, the water storage box 20 can scoop up water from the cooling tank 29, thereby enabling the stirring plate 19 to lift more cooling water and improve working efficiency.

[0043] The lower cavity 10 has a duct 21 on its side wall, and a fan 22 is installed inside the duct 21. The fan 22 blows air into the water in the lower cavity 10. The duct 21 has louvers 23 at its opening, which are used to adjust the airflow direction. With the above configuration, the fan 22 blows air into the cooling water, increasing the airflow speed around the cooling water, thereby increasing the cooling speed of the cooling water.

[0044] The louver 23 is equipped with a rotating shaft 24. One end of the rotating shaft 24 extends out of the air duct 21 and is connected to a gear 25. A rack 26 is slidably connected to the side wall of the air duct 21, and the rack 26 meshes with the gear 25. With the above configuration, the operator can drive several gears 25 to rotate synchronously by sliding the rack 26 up and down, thereby driving the rotating shaft 24 to rotate, and then driving the louver 23 to rotate, thus achieving the purpose of adjusting the airflow direction.

[0045] The side wall of the duct 21 is provided with a sliding groove 27, and the rack 26 is provided with a sliding plate 28, which slides in conjunction with the sliding groove 27. With the above arrangement, the rack 26 can slide more smoothly on the duct 21 through the cooperation of the sliding groove 27 of the sliding plate 28.

[0046] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A water-cooling device suitable for a PCR instrument, characterized in that, include: A base (1) is provided with a mounting groove (2) for placing a perforated plate (3). An annular groove (4) is provided at the opening of the mounting groove (2). An insert plate (5) is provided on the perforated plate (3) and is inserted into the annular groove (4). Cooling tank (29) is connected to a water injection pipe (6) and a water return pipe (7). A water pump (8) is connected to the water injection pipe (6). Both the water injection pipe (6) and the water return pipe (7) are connected to the mounting groove (2). The connection position of the water return pipe (7) to the base (1) is higher than the connection position of the water injection pipe (6) to the base (1). The bottom of the perforated plate (3) can be immersed in cooling water. A partition (30) is located inside the cooling box (29) and divides the internal space of the cooling box (29) into an upper chamber (9) and a lower chamber (10). The return water pipe (7) is connected to the side wall of the upper chamber (9), and the water injection pipe (6) is connected to the side wall of the lower chamber (10). The partition (30) is provided with several leakage holes (11). The top of the cooling box (29) is provided with a material inlet (12), which is used to inject crushed ice into the upper chamber (9). The lower cavity (10) is provided with a rotating rod (17), one end of which extends out of the cooling box (29) and is connected to a drive motor (18). The drive motor (18) is used to drive the rotating rod (17) to rotate around its own axis. Several stirring blades (19) are connected to the side wall of the rotating rod (17). The stirring plate (19) is provided with a water storage box (20), which is used to scoop up water from the cooling tank (29).

2. The water-cooling device suitable for a PCR instrument according to claim 1, characterized in that, The lower cavity (10) has a first cooling plate (13) on one side wall in the vertical direction and a second cooling plate (14) on the other side wall in the vertical direction. The first cooling plate (13) and the second cooling plate (14) are spaced apart, and cooling water can flow through the first cooling plate (13) and the second cooling plate (14) to the bottom of the cooling box (29).

3. A water-cooling device suitable for a PCR instrument according to claim 2, characterized in that, Both the first cooling plate (13) and the second cooling plate (14) are provided with several water guide grooves (15).

4. A water-cooling device suitable for a PCR instrument according to claim 1, characterized in that, A sealing gasket (16) is provided inside the annular groove (4).

5. A water-cooling device suitable for a PCR instrument according to claim 1, characterized in that, The lower cavity (10) has a duct (21) on its side wall, and a fan (22) is provided inside the duct (21). The fan (22) is used to blow air into the water in the lower cavity (10). A louver (23) is provided at the opening of the duct (21). The louver (23) is used to adjust the air direction.

6. A water-cooling device suitable for a PCR instrument according to claim 5, characterized in that, The louver (23) is provided with a rotating shaft (24), one end of which extends out of the air duct (21) and is connected to a gear (25). The side wall of the air duct (21) is slidably connected to a rack (26), and the rack (26) meshes with the gear (25).

7. A water-cooling device suitable for a PCR instrument according to claim 6, characterized in that, The side wall of the air duct (21) is provided with a sliding groove (27), and the straight rack (26) is provided with a sliding piece (28), which slides in conjunction with the sliding groove (27).

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