A convenient detection kit

Abstract

The present invention discloses a convenient detection kit. The present invention includes a main body shell, the upper portion of which is rotatably connected to a drive ring. Each mechanism is manually driven to facilitate detection. The purification mechanism gradually deepens the spiral line to enable more comprehensive and efficient specific identification and binding of magnetic beads and nucleic acid molecules. This solves the problem of insufficient binding caused by simply mixing the two together, poor self-test kit detection effect, and high false detection rate. The amplification detection mechanism undergoes multiple cycles of amplification, thereby achieving amplification to a sufficient concentration and detection even at low concentrations, thereby achieving high detection accuracy and low false detection rate.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro detection equipment, and in particular to a portable detection box. Background Art

[0002] In vitro testing equipment obtains clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.), and then determines the product of disease or body function. However, the current convenient in vitro testing devices often have the problem of inaccurate testing. The degree of purification of each box is insufficient at the same volume, and the number of amplifications is also small, which greatly increases the inaccuracy of the test and leads to frequent misjudgments. Summary of the Invention

[0003] Aiming at the problems existing in the prior art, the present invention proposes a portable detection box.

[0004] The technical solution adopted by the present invention to solve its technical problem is:

[0005] The present invention includes a main body shell, the upper part of which is rotatably connected to a drive ring, and a lysis mechanism is arranged inside the drive ring; trigger rods are evenly distributed and fixedly connected on the lower end surface of the drive ring, and the lower ends of the trigger rods extend into arc-shaped grooves arranged at the edge of the purification block, and the purification mechanism is arranged in the purification block; an amplification block is fixedly arranged at the bottom of the main body shell, and the center position of the amplification block is connected to the detection seat arranged at the lower end of the main body shell through a detection channel, thereby forming an amplification detection mechanism as a whole.

[0006] Preferably, the lysis mechanism structure is as follows: a lysis liquid placement cavity for placing a lysis reagent is provided inside the driving ring, a lysis block is slidably connected to the inside of the lysis liquid placement cavity, the upper end of the lysis block is fixedly connected to the main body shell, a cavity is provided inside the lysis block, and a lysis liquid flow hole communicating with the inside and outside is provided on the lysis block. The bottom wall of the cavity inside the lysis block is high on one side and low on the other side, and an opening is provided at the lowest point. A separation port is also provided on the driving ring below the opening, and the separation port can correspond to its opening as the driving ring rotates. A macromolecular filtration membrane is provided at the opening, and a sample inlet communicating with the outside is provided at the upper end of the lysis block.

[0007] Preferably, the purification mechanism structure is as follows: a purification spiral groove with a groove gradually deepening from the outside to the inside is provided on the upper end surface of the purification block, the initial end of the purification spiral groove is opposite to the opening at the lower end of the lysis block through a guide plate, the middle part of the purification spiral groove is connected to the separation chamber inside it through a purification port, the bottom wall of the separation chamber is inclined, a magnet and a through hole are provided at a higher position, the lower end of the through hole is connected to an amplification tube, magnetic beads are provided in the purification spiral groove, the edge of the purification block is provided with an arc groove with a certain arc length, the arc groove is slidably connected to the trigger rod, a cavity is provided on the side wall of the separation chamber, an elution capsule is provided in the cavity, a push block is fixedly connected to the outer end of the elution capsule, the push block extends into the arc groove, and the nozzle of the elution capsule extends into the separation chamber.

[0008] Preferably, the structure of the amplification detection mechanism is as follows: the amplification block is fixedly connected to the bottom wall of the main shell, and the amplification block is provided with an amplification spiral groove with a spiral line as the path and the depth gradually deepens from the outside to the inside. A variety of temperature plates are provided inside the amplification spiral groove to complete multiple amplification cycles of high-temperature denaturation, low-temperature annealing (renaturation) and suitable temperature extension, thereby completing amplification. The center of the amplification block is connected to the fluorescent reaction chamber in the detection seat through a detection channel, and a light source is provided on the bottom wall of the fluorescent reaction chamber.

[0009] The present invention has the following beneficial effects: Each mechanism is manually driven to facilitate convenient detection. The purification mechanism gradually deepens the spiral to achieve more comprehensive and efficient specific recognition and binding of magnetic beads and nucleic acid molecules, reversing the traditional practice of simply mixing the two, which results in insufficient binding, poor self-test results, and a high false positive rate. The amplification detection mechanism undergoes multiple cycles of amplification, enabling amplification to a sufficient concentration for detection even at low concentrations, resulting in high detection accuracy and a low false positive rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0011] Figure 2 It is a schematic diagram of the overall structure of the present invention from another angle;

[0012] Figure 3 yes Figure 1 Main view;

[0013] Figure 4 yes Figure 1 Side view;

[0014] Figure 5 yes Figure 1 A top view of

[0015] Figure 6 yes Figure 3 Schematic diagram of the structure of "AA";

[0016] Figure 7 yes Figure 3 Schematic diagram of the structure of "CC";

[0017] Figure 8 yes Figure 4 Schematic diagram of the structure of "BB".

[0018] Among them, 10. Rack, 11. Sample inlet, 12. Drive ring, 13. Main body shell, 14. Detection seat, 15. Indicator light, 16. Lysis block, 17. Lysis solution flow hole, 18. Purification block, 19. Push block, 20. Elution capsule, 21. Purification port, 22. Purification spiral groove, 23. Trigger rod, 24. Amplification spiral groove, 25. Fluorescence reaction chamber, 26. Separation chamber, 27. Lysis solution placement chamber, 28. Arc groove, 29. Amplification tube, 30. Separation port, 31. Amplification block, 32. Detection channel. DETAILED DESCRIPTION

[0019] The technical solution of the present invention will be further described below with reference to the accompanying drawings and through specific implementation methods.

[0020] like Figure 1-8 As shown, this embodiment includes a main housing 13, the upper portion of which is rotatably connected to a drive ring 12, and a lysis mechanism is provided inside the drive ring 12; trigger rods 23 are evenly distributed and fixedly connected to the lower end surface of the drive ring 12, and the lower ends of the trigger rods 23 extend into arc-shaped grooves 28 provided at the edge of a purification block 18, in which a purification mechanism is provided; an amplification block 31 is fixedly provided at the bottom of the main housing 13, and the center position of the amplification block 31 is connected to the detection seat 14 provided at the lower end of the main housing 13 through a detection channel 32, thereby forming an amplification detection mechanism as a whole.

[0021] The lysis mechanism: a lysis liquid placement chamber 27 for placing a lysis reagent is provided inside the driving ring 12, a lysis block 16 is slidably connected to the inside of the lysis liquid placement chamber 27, the upper end of the lysis block 16 is fixedly connected to the main shell 13, a cavity is provided inside the lysis block 16, and a lysis liquid flow hole 17 communicating with the inside and outside is provided on the lysis block 16, one side of the bottom wall of the cavity inside the lysis block 16 is high and the other side is low, and an opening is provided at the lowest point, and a separation port 30 is further provided on the driving ring 12 on the lower side of the opening, and the separation port 30 can correspond to its opening as the driving ring 12 rotates, and a macromolecular filtration membrane is provided at the opening, and a sample inlet 11 communicating with the outside is provided at the upper end of the lysis block 16.

[0022] When testing is required, the sample is placed into the lysis block 16 through the sample inlet 11, and the drive ring 12 is then rotated at an angle. At this point, the lysis solution flow hole 17 is connected to the lysis solution placement chamber 27. The lysis solution in the lysis solution flow hole 17 will mix with the sample in the lysis block 16, lysing it and releasing the nucleic acid substances inside. After a certain reaction time, the drive ring 12 is rotated again so that the separation port 30 is aligned with the opening at the lowest point of the lysis block 16. At this time, the substances in the lysis block 16 are filtered through the filter membrane and fall onto the purification mechanism below.

[0023] The purification mechanism: A purification spiral groove 22 is provided on the upper end surface of the purification block 18, which is gradually deepened from the outside to the inside. The initial end of the purification spiral groove 22 is opposite to the opening at the lower end of the lysis block 16 through a guide plate. The middle part of the purification spiral groove 22 is connected to the separation chamber 26 inside it through a purification port 21. The bottom wall of the separation chamber 26 is inclined, and a magnet and a through hole are provided at a higher position. The lower end of the through hole is connected to an amplification tube 29. Magnetic beads are provided in the purification spiral groove 22. An arc groove 28 with a certain arc length is provided at the edge of the purification block 18. The arc groove 28 is slidably connected to the trigger rod 23. A cavity is provided on the side wall of the separation chamber 26, and an elution capsule 20 is provided in the cavity. The outer end of the elution capsule 20 is fixedly connected to a push block 19, and the push block 19 extends into the arc groove 28. The nozzle of the elution capsule 20 extends into the separation chamber 26.

[0024] When the lysis mechanism is completed, the sample flows through the guide plate to the initial end of the outer side of the purification spiral groove 22. As the sample flows, the nucleic acid molecules will gradually specifically bind to the magnetic beads located in the purification spiral groove 22. Due to the long distance, the binding is sufficient, and the problem of missing nucleic acid molecules is basically eliminated. After the sample completely falls into the separation chamber 26, the magnetic beads are adsorbed by the magnet on the upper side of the amplification tube 29 under the action of the magnet. Then, the drive ring 12 is rotated again. The rotation of the drive ring 12 causes the trigger rod 23 to contact the inclined surface of the push block 19, thereby causing the push block 19 to squeeze the elution capsule 20 to eject the eluent therein, thereby washing away the nucleic acid molecules adsorbed by the magnetic beads and causing them to fall into the amplification tube 29.

[0025] The amplification detection mechanism: the amplification block 31 is fixedly connected to the bottom wall of the main shell 13, and the amplification block 31 is provided with an amplification spiral groove 24 with a spiral line as the path depth gradually deepening from the outside to the inside. A variety of temperature plates are provided inside the amplification spiral groove 24 to complete multiple amplification cycles of high-temperature denaturation, low-temperature annealing (renaturation) and suitable temperature extension, thereby completing amplification. The center of the amplification block 31 is connected to the fluorescent reaction chamber 25 through the detection channel 32, and a light source is provided on the bottom wall of the fluorescent reaction chamber 25.

[0026] After the sample is purified and eluted and falls into the amplification block 31, it will gradually flow into the fluorescent reaction chamber 25 as the depth of the amplification spiral groove 24 gradually increases, and will undergo a large amount of amplification with multiple cycles of amplification. When entering the fluorescent reaction chamber 25, the result is judged by the intensity of the fluorescent reaction.

[0027] The indicator light 15 in this embodiment is electrically connected to the detection element in the fluorescent reaction chamber 25 to display the detection result.

[0028] In this embodiment, a sealing component is provided on the sample inlet 11 to ensure that the internal environment is not damaged.

[0029] The temperature of the temperature plate provided in the amplification block 31 in this embodiment is 65°C and 94°C.

[0030] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the concept and scope of the present invention. Any modifications and improvements to the technical solution of the present invention made by a person of ordinary skill in the art without departing from the design concept of the present invention shall fall within the scope of protection of the present invention. The technical content for which protection is sought in the present invention is fully set forth in the claims.

Claims

1. A portable detection kit, characterized in that: The invention comprises a main body shell (13), wherein the upper part of the main body shell (13) is rotatably connected to a driving ring (12), and a lysis mechanism is arranged inside the driving ring (12); trigger rods (23) are evenly distributed and fixedly connected on the lower end surface of the driving ring (12), and the lower end of the trigger rod (23) extends into an arc-shaped groove (28) arranged at the edge of a purification block (18), and the purification mechanism is arranged in the purification block (18); an amplification block (31) is fixedly arranged at the bottom of the main body shell (13), and the center position of the amplification block (31) is connected to the detection seat (14) arranged at the lower end of the main body shell (13) through a detection channel (32), thereby forming an amplification detection mechanism as a whole; A lysis solution placement cavity (27) for placing a lysis reagent is provided inside the driving ring (12), a lysis block (16) is slidably connected to the inside of the lysis solution placement cavity (27), the upper end of the lysis block (16) is fixedly connected to the main body shell (13), a cavity is provided inside the lysis block (16), and a lysis solution flow hole (17) communicating with the inside and outside is provided on the lysis block (16), one side of the bottom wall of the cavity inside the lysis block (16) is higher than the other side, and an opening is provided at the lowest point, and a separation port (30) is further provided on the driving ring (12) below the opening, and the separation port (30) can correspond to its opening as the driving ring (12) rotates, and a macromolecular filtration membrane is provided at the opening, and a sample inlet (11) communicating with the outside is provided at the upper end of the lysis block (16); The purification block (18) is provided with a purification spiral groove (22) on the upper end surface thereof, which is gradually deepened from the outside to the inside. The initial end of the purification spiral groove (22) is directly opposite to the opening at the lower end of the lysis block (16) through a guide plate. The middle of the purification spiral groove (22) is connected to the separation chamber (26) inside it through a purification port (21). The bottom wall of the separation chamber (26) is inclined, and a magnet and a through hole are provided at a higher position. The lower end of the through hole is connected to an amplification tube (29). (22) is provided with magnetic beads, an arc-shaped groove (28) of a certain arc length is provided at the edge of the purification block (18), the arc-shaped groove (28) is slidably connected to the trigger rod (23), a cavity is provided on the side wall of the separation chamber (26), an elution capsule (20) is provided in the cavity, a push block (19) is fixedly connected to the outer end of the elution capsule (20), the push block (19) extends into the arc-shaped groove (28), and the nozzle of the elution capsule (20) extends into the separation chamber (26); The amplification block (31) is provided with an amplification spiral groove (24) whose depth gradually deepens from the outside to the inside with a spiral line as the path. A variety of temperature plates are provided inside the amplification spiral groove (24) to complete multiple amplification cycles of high-temperature denaturation, low-temperature annealing and suitable temperature extension, thereby completing amplification. The center of the amplification block (31) is connected to the fluorescent reaction chamber (25) in the detection seat (14) through the detection channel (32), and a light source is provided on the bottom wall of the fluorescent reaction chamber (25).

2. A portable detection kit according to claim 1, characterized in that: The detection element in the fluorescent reaction chamber (25) is electrically connected to the indicator light (15) to display the detection result.

3. The portable detection kit according to claim 1, characterized in that: The sample inlet (11) is provided with a sealing component to ensure that the internal environment is not damaged.

4. The portable detection kit according to claim 1, characterized in that: The temperature of the temperature plate provided in the amplification block (31) is 65°C or 94°C.

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