A method for detecting carbendazim residue based on personal blood glucose meter
By coupling single-chain antibodies and alkaline phosphatase on AuNPs and reading glucose concentration using a personal glucose meter, the problem of traditional germine detection methods relying on large equipment and complex operations is solved, and a portable, highly sensitive germine detection is achieved.
CN115219716BActive Publication Date: 2025-03-18SOUTHWEST UNIV
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Patent Information
- Application Number
- CN202210781736.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-07-04
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2042-07-04
AI Technical Summary
Technical Problem
Traditional Bacteriasis residue detection methods require large equipment and complex sample pretreatment processes, limiting their application in rapid screening.
Method used
By coupling single-chain antibodies (sAb) and alkaline phosphatase (ALP) on AuNPs, glucose is generated using ALP to catalyze the dephosphorylation of glucose-1-phosphate disodium salt, and glucose concentration is read using a personal glucose meter (PGM), to achieve portable quantitative detection of pyrimidine.
Benefits of technology
This method realizes fast and portable Bacterine detection, overcomes the shortcomings of traditional methods relying on large equipment and complex operations, has high sensitivity and specificity, and is suitable for rapid on-site detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses a method for detecting carbendazim residues based on a personal blood glucose meter. The present application is an improvement on the traditional ELISA method. sAb and ALP are coupled to AuNPs at the same time. ALP catalyzes the dephosphorylation of G‑1‑P to generate glucose molecules. PGM is used as a signal reading tool to realize the portable quantitative detection of CBD. The present method utilizes the high specific surface area of AuNPs and the dual signal amplification strategy of TdT catalyzing the extension of single-stranded DNA to realize the effective amplification of the detection signal, so that the present method has a high sensitivity. Since the present method is a detection method constructed based on the specific recognition of antigens and antibodies, it has good specificity and strong anti-interference ability. The present method realizes the portable quantitative detection of CBD by PGM, overcoming the disadvantage that the traditional ELISA can only be accurately quantitatively analyzed with the help of large instruments and equipment such as microplate readers. Therefore, it is very suitable for on-site rapid detection and analysis, and has a high application potential in resource-scarce areas.
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