A device for recording eye movements, EEG and EMG of experimental animals during sleep

Abstract

The present invention belongs to the field of experimental scientific research technology, and specifically relates to a device for recording eye movement, electroencephalogram, and electromyography during sleep of experimental small animals, which is composed of a magnetic displacement sensor, a recording silver wire, a PCB base plate, a PCB connecting plate, and an FPC connecting interface. The sensor and the recording silver wire are simultaneously connected to the front of the PCB base plate, the PCB connecting plate is vertically welded to the back of the base plate, and the FPC connecting interface is welded to the PCB connecting plate. The device is small and light, has good stability, is convenient for data export, is simple to manufacture and has low cost, and can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movement.

Description

Technical Field

[0001] The present invention belongs to the field of experimental scientific research technology, and specifically relates to a device for recording eye movements, electroencephalograms, and electromyography of experimental small animals during sleep. The device is small and light, convenient for data export, simple to manufacture, and low in cost, and can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movements. Background Art

[0002] According to research, in the current scientific research on sleep, in humans, EEG, electromyography, eye movement and other data can be collected, but for experimental mice or other small experimental animals, most of them are mainly based on EEG and electromyography data, because the experimental animals will close their eyes during sleep and the eyeballs of experimental mice are too small, which often makes it very difficult to collect eye movement data. Therefore, there are few reports on the collection of eye movement data of experimental animals. At present, in the relevant experiments in this technical field, the recording of eye movement data is mainly carried out on humans or large primates for recording and data analysis, and the current recording of eye movement data is mainly EOG and high-speed cameras to identify the pupil for recording. If recording during sleep, only EOG can be used, but because the position of the EOG recording electrode is not accurate to the extraocular muscles, the recorded data often has some signal interference, and it will affect the free movement of experimental mice. The industry has paid attention to exploring the significance of eye movement in experimental mice and its connection with sleep.

[0003] Based on the current state of the art, the inventor of this application intends to provide a device for recording eye movements, EEG, and EMG during sleep in small animals. The device can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movements. Summary of the invention

[0004] The purpose of the present invention is to provide a device for recording eye movements, EEG and EMG of experimental small animals during sleep based on the current state of the art. The device can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movements.

[0005] The present invention provides a device for recording eye movement, EEG and EMG during sleep of experimental small animals. The present invention concentrates EEG, EMG and eye movement signals on a small and light device by designing circuit boards and interfaces, etc. All relevant signals can be simultaneously input into a computer for recording through a thin FPC cable. It can be used to record eye movement, EEG and EMG data during free activities, closed-eye sleep and other processes of experimental mice or other experimental small animals, and further explore the significance of eye movement of experimental mice and other experimental small animals and their connection with sleep. The device is small and light, data export is convenient, and the production is simple and low-cost. It can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movement.

[0006] Specifically, the device for recording eye movements, electroencephalograms, and electromyography of experimental small animals during sleep of the present invention is composed of a magnetic displacement sensor 1, a recording silver wire, a PCB base plate 3, a PCB connecting plate 4, and an FPC connecting interface 5. The sensor and the recording silver wire are simultaneously connected to the front side of the PCB base plate, the PCB connecting plate is then vertically welded to the back side of the base plate, and the FPC connecting interface is then welded to the PCB connecting plate.

[0007] In the present invention, the PCB substrate can be connected to three signal sources, namely, EEG, EMG and sensor, at the same time, and signals of a total of eight channels can be recorded simultaneously.

[0008] In the present invention, the welding positions of the EEG and EMG signals on the PCB substrate can ensure that the signals do not affect each other, thereby greatly reducing the size of the PCB and improving operability and convenience.

[0009] In the present invention, the connection between the PCB connecting plate and the PCB base plate is vertical double-sided welding, which is beneficial to saving space and stabilizing the signal output port.

[0010] In the present invention, the PCB connecting board selects the FPC interface as the signal output port, ensuring that the connecting line is an FPC cable, which is lighter and is conducive to the free movement of experimental small animals and the long-term recording of signals.

[0011] More specifically, the device for recording eye movement, EEG and EMG of experimental small animals during sleep of the present invention mainly consists of five parts: sensor chip 1, EEG and EMG silver wire 2, PCB base board 3, PCB connecting board 4 and FPC interface 5.

[0012] Among them, two EEG and EMG silver wires are welded to the PCB base board, the sensor chip is welded on the base board, the PCB connecting board is welded to the back of the base board, the FPC interface is placed at the correct solder point of the PCB connecting board, and finally all signals are transmitted to the computer for recording at the same time through the FPC cable.

[0013] In the present invention, a PCB base plate and a PCB connecting plate are designed. 12 pads are arranged at the bottom of the base plate, including 4 circular pads and 8 square pads. The 4 circular pads are used to weld two EEG electrodes and two EMG electrodes. Four of the 8 square pads are the positive and negative electrodes of the sensor's signal and the positive and negative electrodes of the power supply, and the other four are used to fix the sensor to prevent unstable fixation. The 8 pads for recording signals at the bottom of the base plate are connected to the 8 pads on the back of the base plate through perforations, and the 8 pads on the back are connected to the PCB connecting plate. In the present invention, based on the limited head area of ​​experimental mice and experimental small animals, a connecting plate is designed to be able to be welded upright, which is convenient for fixing the FPC interface, and is also convenient for implanting the experimental mice and experimental small animals and plugging and unplugging the data line.

[0014] In the embodiment of the present invention, two EEG and two EMG silver wires are welded to the PCB substrate, and then covered with a layer of epoxy resin AB glue, and then the sensor chip is aligned with the eight pads on the substrate (such as Figure 3 3, 4, 5, 6, 7 are soldered, where 3456 are ports with specific signals, and the four 7s are just ports for fixing. Then solder the eight solder joints on the back of the PCB connecting board and the base board, and then place the FPC interface at the correct solder joints on the PCB connecting board. After soldering all the solder joints, finally transmit all the signals to the computer through the FPC cable for recording.

[0015] In the embodiment of the present invention, after the manufactured device is tested in vitro, the end of the EEG silver wire is welded to a skull pin with a diameter of 1 mm, and then placed in the skull of the experimental mouse, the end of the EMG silver wire is inserted into the neck muscle of the experimental mouse, and the bottom of the entire device, that is, the sensor is vertically placed in the center of the line connecting the two eyeballs of the experimental mouse, and dental cement is used to cover the exposed skull pin and silver wire, and biological glue is applied to the damaged neck muscle to fix it. After the operation is completed, the relevant data can be recorded after the experimental mouse recovers for one week.

[0016] The device of the present invention combines EEG, EMG and eye movement recordings, and can use anisotropic magnetoresistive sensors to record eye movements of experimental animals during sleep, providing a recording method for recording eye movements, EEG and EMG during sleep of experimental animals, thereby better combining basic scientific research on sleep and eye movements, and providing a feasible method for exploring the significance of eye movements in sleep.

[0017] The present invention also has the advantages of light connecting wires, and the sensor only needs to be fixed to the brain, which basically does not affect the daily behavior of the experimental animals. Comparative experiments have confirmed that the sensor of the device and the eye movement signal recorded by a high-speed camera at 50 frames per second have significantly good matching, and it can be operated for a long time of at least two months after being implanted on the top of the head of the experimental animal.

[0018] The device of the present invention provides a convenient method for recording the eye movements of experimental small animals during sleep in a closed-eye state. The device is small and light, convenient for data export, stable, firm, low in signal noise, high in temporal and spatial resolution, simple to manufacture and low in cost. It can provide a new and easy method for basic scientific research to explore the relationship between sleep and eye movements. It has significant practical value in the field of sleep and eye movement research. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of the device, where

[0020] Figure 1Among them, 1 is the anisotropic magnetoresistive sensor, 2 is the pads for EEG and EMG, 3 is the independently designed PCB base board, which serves to connect the EEG, EMG and eye movement sensors, 4 is the independently designed PCB connecting board, which serves to connect 3 and the FPC interface, 5 is the eight-channel FPC interface, and 6 is the FPC cable.

[0021] Figure 2 is the design drawing of the PCB substrate of the device, in which:

[0022] Figure 2 On the left, 1 is the pad for myoelectricity, 2 is the pad for electroencephalography, and silver wires can be soldered to these four pads to record EEG and EMG. 3 and 6 are signal output pads, 4 is the ground pad, 5 is the positive voltage pad, 7 is a fixed pad with no actual signal output, 8 is the back of the sensor's four pads with perforated connections, and 9 is the back of the perforated connections for electroencephalography and myoelectricity pads;

[0023] Figure 2 On the right is the design drawing of the FPC interface PCB connection board. 1 is the FPC interface, 2 is the fixed pad on the FPC interface, 3 is the PCB board body, 4 is the pad interface for EEG, EMG and eye movement signals, and 5 is the pad connected to the base board.

[0024] Figure 3 is a physical diagram of the device, wherein:

[0025] From left to right are the magnet, sensor, PCB base plate, PCB connecting plate, and FPC interface physical pictures.

[0026] Figure 4 This is a schematic diagram of recording EEG, EMG and eye movement signals in mice, where:

[0027] The upper middle is the sensor power supply device, the upper right corner is an amplifier that can be used to amplify the signal, and the computer screen in the lower right corner can be directly recorded using the Spike2 software. EEG is an example of electroencephalogram recording, EMG is an example of electromyography recording, and sensor is an example of eye movement signal recording.

[0028] Figure 5 It is an example of the device being implanted in an experimental mouse and a complete physical picture of the device. DETAILED DESCRIPTION

[0029] Example 1

[0030] like Figure 1 As shown, the device mainly includes a sensor chip 1, an EEG and myoelectric silver wire 2, a PCB base plate 3, a PCB connecting plate 4, and an FPC interface 5. The two EEG and two myoelectric silver wires are welded to the PCB base plate (as shown in FIG. Figure 31 and 2 in the figure), and then cover it with a layer of epoxy resin AB glue, and then align the sensor chip with the eight pads on the base plate (as shown in Figure 3 As shown in the third picture, 3, 4, 5, 6, 7 are soldered, where 3456 are ports with specific signals, and the four 7s are just ports for fixing. Then solder the eight solder points on the back of the PCB connecting board and the base board, as shown in ( Figure 3 The 5 in the fourth figure (as shown) is the welding point between the PCB connecting board and the base board. Then the FPC interface is placed at the correct soldering point of the PCB connecting board, all the soldering points are soldered, and finally, all the signals are transmitted to the computer through the FPC cable at the same time for recording.

[0031] Example 2

[0032] like Figure 5 As shown, after the device is made and tested in vitro, the end of the EEG silver wire is welded to a skull pin with a diameter of 1 mm, and then screwed into the skull of the experimental mouse. The end of the EMG silver wire is inserted into the neck muscle of the experimental mouse. Then the bottom of the entire device, that is, the sensor is placed vertically in the center of the line connecting the two eyeballs of the experimental mouse. Dental cement is used to cover the exposed skull pin and silver wire, and biological glue is applied to the injured neck muscle of the experimental mouse to fix it. After the operation is completed, the data can be recorded after the experimental mouse recovers for one week.

Claims

1. A device for recording eye movements, EEG and EMG of experimental animals during sleep, characterized in that: It consists of a magnetic displacement sensor, a recording silver wire, a PCB base plate, a PCB connecting plate, and an FPC connecting interface. The sensor and the recording silver wire are simultaneously connected to the front side of the PCB base plate, the PCB connecting plate is vertically welded to the back side of the base plate, and the FPC connecting interface is welded to the PCB connecting plate. The bottom of the PCB substrate is provided with 12 pads, including 4 circular pads and 8 square pads. The 4 circular pads are used to weld two EEG electrodes and two EMG electrodes, and four of the 8 square pads are the positive and negative electrodes of the sensor signal and the positive and negative electrodes of the power supply, and the other four are used to fix the sensor; The connection between the PCB connecting plate and the PCB base plate is vertical double-sided welding, which is beneficial to saving space and stabilizing the signal output port.

2. The device for recording eye movements, EEG and EMG of experimental small animals during sleep according to claim 1, characterized in that: The 8 pads for recording signals at the bottom of the base plate are connected to the 8 pads on the back of the base plate through perforations, and the 8 pads on the back are connected to the PCB connection board.

3. The device for recording eye movements, EEG and EMG of experimental small animals during sleep according to claim 1, characterized in that: The PCB substrate is connected to three signal sources, namely, electroencephalogram, electromyography and sensor, and signals of a total of eight channels are recorded simultaneously.

4. The device for recording eye movements, EEG and EMG of experimental animals during sleep according to claim 1, characterized in that: The PCB connecting board selects the FPC interface as the signal output port, ensuring that the connecting line is an FPC cable, which is conducive to the free movement of experimental animals and long-term recording of signals.

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