A delivery system and delivery method

Abstract

This invention discloses a delivery system and delivery method. The delivery system includes a microfluidic chip and a control instrument. The microfluidic chip includes a chip body, a nanoporous membrane, and a loading electrode. The control instrument includes an instrument body and an electronic control component installed within the instrument body. The instrument body has a holding portion for mounting the microfluidic chip, and the output end of the electronic control component extends to the holding portion. When the microfluidic chip is mounted in the holding portion, the output end of the electronic control component is electrically connected to the loading electrode. When the electronic control component is powered, it forms a closed loop with the microfluidic chip and the portion to be delivered, driving charged substances in the liquid to be delivered through the connecting holes and the nanoporous membrane to the portion to be delivered. This allows the liquid to be delivered into the skin and cells without piercing the skin, improving delivery efficiency. In other words, this invention avoids skin damage while improving delivery efficiency.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a delivery system and delivery method. Background Technology

[0002] Beauty devices are popular because they can modify and improve skin condition. The main purpose and principle of beauty devices is to achieve the effect by introducing specific beauty essence molecules into the skin and cells. However, the beauty products on the market are currently highly homogenized and of varying quality. Some cause serious damage to the skin, some have poor absorption effects, and some are cumbersome and complicated to operate. Overall, the effective absorption of beauty essence molecules is an important problem currently facing beauty devices on the market.

[0003] Currently, the commonly used method is microneedling, which uses tiny needle-like structures to damage and destroy the skin structure and cell tissue. Then, beauty essence is applied to penetrate into the skin and cells. This method requires damaging the skin as a prerequisite. Its disadvantages are that it may cause irreparable skin damage or cause wound infection. At the same time, the penetration efficiency of beauty essence is relatively limited and the efficiency is relatively low. Summary of the Invention

[0004] In view of this, the first objective of the present invention is to provide a delivery system designed to avoid damage to the skin while improving delivery efficiency.

[0005] A second objective of this invention is to provide a delivery method.

[0006] To achieve the first objective mentioned above, the present invention provides the following solution:

[0007] A delivery system includes a microfluidic chip and control instruments;

[0008] The microfluidic chip includes a chip body, a nanoporous membrane, and a loading electrode. The chip body has an inner cavity for holding the liquid to be introduced. The loading electrode is mounted on the chip body, and one end of the loading electrode extends into the inner cavity of the chip body. A communicating hole is opened at the first end of the chip body to communicate with the inner cavity of the chip body. The nanoporous membrane is encapsulated at the first end of the chip body for contacting the part to be introduced.

[0009] The control instrument includes an instrument body and an electronic control component installed in the instrument body. The instrument body is provided with a holding part for mounting the microfluidic chip, and the output end of the electronic control component extends to the position of the holding part.

[0010] When the microfluidic chip is installed in the container, the output terminal of the electronic control component is electrically connected to the loading electrode. When the electronic control component is powered, the electronic control component, the microfluidic chip, and the container to be introduced form a closed loop to drive the charged substance in the liquid to be introduced to be delivered into the container through the connecting hole and the nanoporous membrane.

[0011] In one specific implementation, the electronic control component includes a power supply line, a circuit board, a first connecting electrode, a second connecting electrode, and an external electrode;

[0012] The power supply lines are electrically connected to the circuit board, the first connecting electrode, and the second connecting electrode, respectively.

[0013] The external electrode is arranged circumferentially around the microfluidic chip. The number of the first connecting electrode and the second connecting electrode is at least one. When the microfluidic chip is installed in the holding part, the first connecting electrode is electrically connected to the loading electrode, and the second connecting electrode is electrically connected to the external electrode.

[0014] In another specific implementation, the main body of the instrument includes a mounting shell, an external electrode mounting base, a connecting electrode mounting base, and a loading and unloading device;

[0015] The power supply line and the circuit board are both installed in the inner cavity of the mounting shell, and the mounting shell is provided with a mounting cylinder that communicates with the inner cavity of the mounting shell;

[0016] The connecting electrode mounting base is installed inside the mounting cylinder, and the holding part is a holding groove formed inside the connecting electrode mounting base;

[0017] The external electrode mounting base is installed inside the mounting cylinder and sleeved outside the connecting electrode mounting base;

[0018] The external electrode is mounted on the external electrode mounting base, sleeved outside the connecting electrode mounting base, and arranged around the holding groove;

[0019] Both the first connecting electrode and the second connecting electrode are mounted on the connecting electrode mounting base. The first connecting electrode extends into the holding groove and is used to abut against the loading electrode to achieve electrical connection when the microfluidic chip is installed in the holding groove. The second connecting electrode is used to abut against the external electrode to achieve electrical connection.

[0020] The loading and unloading device is installed inside the mounting housing and is used to lock or unlock the microfluidic chip into the holding slot.

[0021] In another specific embodiment, the connecting electrode mounting base includes a first mounting platform, a second mounting platform, and a holding cylinder with an inner cavity forming a holding groove;

[0022] The container is mounted on the second mounting platform, and the second mounting platform is mounted on the first mounting platform;

[0023] Along the direction from the bottom end to the top end of the mounting cylinder, a first limiting step and a second limiting step are provided at intervals inside the mounting cylinder. The end face of the first mounting platform facing the second mounting platform abuts against the step surface of the first limiting step and is fastened together by fasteners.

[0024] The container is provided with a mounting platform, and the mounting platform is provided with a first electrode mounting hole for mounting the first connecting electrode.

[0025] The outer electrode mounting base is annular, and its top end abuts against the step surface of the second limiting step. The inner wall of the outer electrode mounting base is provided with an internal thread, and the outer wall of the outer electrode is provided with an external thread that connects with the internal thread of the outer electrode mounting base.

[0026] The second mounting platform has a second electrode mounting hole for mounting the second connecting electrode, and the second connecting electrode abuts against the threaded connection between the outer electrode mounting base and the outer electrode.

[0027] The first connecting electrode is installed inside the container.

[0028] In another specific embodiment, both the first connecting electrode and the second connecting electrode are elastic electrodes;

[0029] and / or

[0030] Along the axial direction of the mounting cylinder, the inner wall of the mounting cylinder and the outer wall of the outer electrode mounting base are provided with a first guide groove on one side and a first guide protrusion that slides in cooperation with the first guide groove on the other side.

[0031] and / or

[0032] The inner wall of the container and the outer wall of the chip body are provided with a second guide groove on one and a second guide protrusion that slides in cooperation with the second guide groove on the other.

[0033] In another specific implementation, the loading and unloading device includes:

[0034] A sleeve, which is fixed inside the mounting housing and has open ends;

[0035] A guide shaft, which is fitted inside the sleeve;

[0036] The locking component includes a locking seat and a locking rod connected to the locking seat. The locking seat is slidably sleeved on the outside of the guide shaft, and one end abuts against a limiting boss provided on the guide shaft. The sleeve has a sliding through hole, and the locking rod extends out of the first sliding through hole and into the holding groove to lock the microfluidic chip through the locking part on the locking rod.

[0037] An elastic reset member is sleeved outside the guide shaft. The first end of the elastic reset member abuts against the end face of the locking seat away from the limiting boss, and the second end of the elastic reset member abuts against the limiting member installed on the guide shaft.

[0038] A drive assembly is slidably mounted inside the sleeve, and the drive end of the drive assembly is connected to the end of the locking seat facing the limiting boss to drive the locking rod to move away from the microfluidic chip, thereby unloading the microfluidic chip.

[0039] In another specific implementation, the number of the locking member, the elastic reset member, the limiting member, and the driving assembly are all two.

[0040] The two locking members abut against each other at both ends of the limiting protrusion, and the two limiting members are respectively mounted on the guide shaft and located at both ends of the limiting protrusion;

[0041] The first ends of the two elastic reset members abut against the two locking members respectively, and the second ends abut against the two limiting members respectively;

[0042] The two drive components are located at the two ends of the guide shaft, and the drive end of any one of the drive components can slide through the locking seat near the drive component and connect to the locking seat away from the drive component.

[0043] In another specific implementation, the driving component includes a driving element;

[0044] The driving component includes a driving seat and a driving rod. The driving seat is slidably installed inside the sleeve. One end of the driving rod is fixed to the driving seat, and the other end of the driving rod can slide through a locking seat near the driving seat and connect to a locking seat away from the driving seat.

[0045] In another specific implementation, the drive component further includes a button;

[0046] The button is installed on the end of the drive seat away from the drive rod, and the button is exposed outside the sleeve.

[0047] In another specific embodiment, the drive assembly further includes a magnet block, and the button has a mounting slot. The magnet block is magnetically mounted in the mounting slot and magnetically connected to the drive base.

[0048] and / or

[0049] The locking part is a hook provided on the locking rod, and the chip body has a slot that engages with the hook;

[0050] and / or

[0051] The locking seat has a receiving groove on the end face away from the limiting boss to limit the first end of the elastic reset member.

[0052] In another specific embodiment, the mounting housing includes a front housing, a rear housing, and a middle housing;

[0053] The two side walls of the rear shell are provided with support grooves to support the sleeve, and the inner cavity of the rear shell is provided with a plurality of spaced first support plates for supporting the battery of the power supply line.

[0054] The middle shell is covered at the top of the rear shell, and the end of the middle shell facing the rear shell is provided with a receiving groove that is recessed in the direction away from the rear shell to accommodate the sleeve. The receiving groove is also provided with a second sliding through hole that allows the locking rod of the delivery system to pass through and slide.

[0055] The top of the middle shell is provided with a terminal block for connecting the circuit board;

[0056] The front shell covers the top of the middle shell, and a display port is provided on the front shell. The circuit board is connected to a display screen, which is installed on the end of the front shell facing the middle shell and is directly opposite the display port. The front shell is also provided with mechanical or electronic buttons that are connected to the circuit board to switch the output mode of the power supply line.

[0057] The mounting cylinder is mounted on the front housing.

[0058] In another specific implementation, the second end of the chip body is provided with a sample inlet communicating with the inner cavity of the chip body for injecting the liquid to be introduced, and the sample inlet is sealed with a sample cap;

[0059] The second end of the chip body is also provided with an exhaust port that communicates with the inner cavity of the chip body, and the exhaust port is encapsulated with a waterproof and breathable membrane.

[0060] and / or

[0061] The chip body includes a cavity and a second support plate;

[0062] One end of the cavity is closed, which is the second end of the chip body;

[0063] The other end of the cavity is open and is the first end of the chip body. The second support plate blocks the open end of the cavity, and the connecting hole is formed on the second support plate.

[0064] The nanoporous membrane is fixed to the end face of the open end of the cavity and has a preset gap with the second support plate.

[0065] In another specific implementation, the number of the connecting holes is multiple, and they are evenly distributed on the second support plate;

[0066] and / or

[0067] The inner wall of the open end of the cavity is provided with a third limiting step for limiting and abutting against the end face of the second support plate;

[0068] The second support plate has a connecting part at the end facing the cavity so as to be detachably connected to the inner wall of the cavity; or, the second support plate and the third limiting step are laser welded together.

[0069] In another specific embodiment, the connecting part includes a connecting plate and a snap-fit ​​protrusion disposed on the connecting plate, the connecting plate is fixed on the second support plate, and the inner wall of the cavity is provided with a snap-fit ​​groove that snaps into the snap-fit ​​protrusion;

[0070] or

[0071] The connecting part includes a connecting plate and a snap-fit ​​groove formed on the connecting plate, and the inner wall of the cavity is provided with snap-fit ​​protrusions for snapping with the snap-fit ​​groove;

[0072] or

[0073] The connecting part is a circular ring plate fixed on the second support plate, and the circular ring plate is provided with an external thread. The cavity is a cylindrical cavity, and the cavity is provided with an internal thread that mates with the external thread.

[0074] and / or

[0075] The second support plate has a limited rotation guide groove on its side wall, and the cavity has a limited rotation guide protrusion located in the limited rotation guide groove on its inner wall;

[0076] and / or

[0077] The sample dispensing cap includes a fastening cap, an elastic cap, and a connector. The two ends of the connector are respectively connected to the fastening cap and the elastic cap. The chip body has a fastening hole, the fastening cap is installed in the fastening hole, and the elastic cap is detachably and sealed in the sample dispensing port.

[0078] and / or

[0079] The waterproof and breathable membrane is connected to the chip body by ultrasonic bonding.

[0080] and / or

[0081] The loading electrode is an inert metal electrode;

[0082] and / or

[0083] The first end of the chip body is provided with a receiving groove, and the bottom end of the receiving groove is provided with a receiving hole that communicates with the inner cavity of the chip body. The axis of the receiving hole coincides with the center of the chip body. The loading electrode includes an electrode platform and an electrode post. The electrode post is perpendicularly connected to the electrode platform, and the end face of the electrode platform facing the electrode post is sealed to the receiving groove. The electrode post passes through the receiving hole and extends beyond the predetermined length of the receiving hole.

[0084] and / or

[0085] The nanoporous membrane is laser-welded to the chip body.

[0086] The various embodiments of the present invention can be combined arbitrarily as needed, and the resulting embodiments are also within the scope of the present invention and are part of the specific implementation of the present invention.

[0087] To achieve the second objective mentioned above, the present invention provides the following solution:

[0088] A delivery method, comprising:

[0089] Provide a delivery system as described in any of the above;

[0090] The liquid to be introduced is loaded into the cavity of the microfluidic chip of the delivery system, and the loaded microfluidic chip is installed into the holding part of the control instrument of the delivery system, so that the output end of the electronic control component of the delivery system is electrically connected to the loading electrode of the microfluidic chip.

[0091] The nanoporous membrane of the microfluidic chip is brought into contact with the part to be introduced;

[0092] The delivery system is activated, and the desired mode is selected. The electrical control component outputs an electrical signal corresponding to the mode to the loading electrode, driving the charged substance in the liquid to be introduced to be delivered through the nanoporous membrane to the part to be introduced.

[0093] The delivery system provided by this invention, taking human skin as an example, involves loading the liquid to be introduced into a microfluidic chip, installing the loaded microfluidic chip onto the holding part of the control instrument, and electrically connecting the output end of the electronic control component to the loading electrode of the microfluidic chip. Next, the nanoporous membrane of the microfluidic chip is brought into contact with the human skin. Then, the delivery system is activated, and the desired mode is selected. The electronic control component outputs an electrical signal corresponding to the mode to the loading electrode, driving the charged substances in the liquid to be introduced through the nanoporous membrane to be delivered into the target area. This invention utilizes an electric field to deliver the liquid to be introduced into the human skin. The instantaneous voltage opens the cell membrane of the phospholipid bilayer in the skin cells, allowing the charged molecules in the liquid to be introduced to be transported from the outside to the inside of the cells. This achieves the introduction of the liquid into the skin and cells without piercing the skin, thus improving the delivery efficiency. In short, this invention avoids damage to the skin while improving delivery efficiency. Attached Figure Description

[0094] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any novel effort.

[0095] Figure 1 A three-dimensional structural schematic diagram of the delivery system provided by the present invention;

[0096] Figure 2 A three-dimensional structural schematic diagram of the control instrument provided by the present invention;

[0097] Figure 3 An exploded structural diagram of the control instrument provided by the present invention;

[0098] Figure 4 A cross-sectional structural schematic diagram of the control instrument provided by the present invention;

[0099] Figure 5 A partial exploded structural diagram of the instrument body provided by the present invention;

[0100] Figure 6 A partial cross-sectional view of the main body of the instrument provided by the present invention;

[0101] Figure 7This is a three-dimensional structural schematic diagram of the loading and unloading device provided by the present invention;

[0102] Figure 8 This is a cross-sectional structural schematic diagram of the loading and unloading device provided by the present invention;

[0103] Figure 9 This is an exploded structural diagram of the loading and unloading device provided by the present invention;

[0104] Figure 10 This is a three-dimensional structural schematic diagram of the microfluidic chip provided by the present invention;

[0105] Figure 11 This is a three-dimensional structural diagram of the microfluidic chip provided by the present invention without the installation of a nanoporous membrane.

[0106] Figure 12 This is an exploded view of the microfluidic chip provided by the present invention.

[0107] Figure 13 This is a schematic diagram of the microfluidic chip provided by the present invention.

[0108] Figure 14 The simulation results of the voltage trend distribution inside the microfluidic chip provided by this invention are shown in the figure.

[0109] Figure 15 The figure shows the simulation results of the internal current density distribution of the microfluidic chip provided by the present invention.

[0110] in, Figures 1-15 middle:

[0111] Delivery system 1000, microfluidic chip 100, control instrument 200, chip body 101, nanoporous membrane 102, loading electrode 103, connecting hole 101a, instrument body 201, electronic control component 202, circuit board 202a, first connecting electrode 202b, second connecting electrode 202c, external electrode 202d, mounting shell 201a, external electrode mounting base 201b, connecting electrode mounting base 201c, loading and unloading device 201d, mounting cylinder 201a-1, holding tank 201c-1, first mounting platform 201c-2, second mounting platform 201c -3, Container 201c-4, First limiting step 201a-1a, Second limiting step 201a-1b, First guide groove 201b-1, Second guide groove 101b, Second guide protrusion 201c-4a, Sleeve 201d-1, Guide shaft 201d-2, Locking component 201d-3, Locking seat 201d-3a, Locking rod 201d-3b, Limiting boss 201d-2a, Locking part 201d-3c, Elastic reset component 201d-4, Limiting component 201d-2b, Drive assembly 201d-5, Drive component 201d-5 a. Drive base 201d-5a-1, Drive rod 201d-5a-2, Button 201d-5b, Magnet block 201d-5c, Slot 101c, Front shell 201a-2, Rear shell 201a-3, Middle shell 201a-4, Support groove 201a-3a, First support plate 201a-3b, Battery 202e, Terminal block 201a-4a, Display port 201a-2a, Display screen 202a-1, Mechanical button 201a-2b, Transparent protective plate 201a-2c, Sample filling port 101d, Sample filling cover 104, Exhaust port 101e Waterproof and breathable membrane 105, cavity 101f, second support plate 101g, third limiting step 101f-1, connecting part 101g-1, connecting plate 101g-1a, snap-fit ​​protrusion 101g-1b, guide plate 101g-2, snap-fit ​​groove 101f-2, rotation limiting guide groove 101g-2, rotation limiting guide protrusion 101f-3, fastening cover 104a, elastic cover 104b, connector 104c, receiving groove 101f-4, electrode platform 103a, electrode post 103b, fastening hole 101f-5, chip model 300, skin layer 400. Detailed Implementation

[0112] The following will refer to the appendices in the embodiments of the present invention. Figures 1-15 The technical solutions in the embodiments of the present invention are clearly and completely described herein. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0113] In the description of this invention, it should be understood that the terms "upper," "lower," "top surface," "bottom surface," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the indicated position or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations of the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0114] Combination Figures 1-15 As shown, the present invention provides a delivery system 1000 that can transport charged liquid to be introduced into human cells through an electric field by utilizing nano-electroporation technology, avoiding damage to the skin and improving the introduction efficiency.

[0115] like Figure 1 As shown, the delivery system 1000 includes a microfluidic chip 100 and a control instrument 200. The microfluidic chip 100 is used to hold the liquid to be introduced. The liquid to be introduced can be a beauty essence or other medicines, which can be selected according to the needs.

[0116] Specifically, such as Figures 10-13 As shown, the microfluidic chip 100 includes a chip body 101, a nanoporous membrane 102, and a loading electrode 103. The chip body 101 has an inner cavity for holding the liquid to be introduced. The shape of the inner cavity of the chip body 101 is not limited and can be set to any shape as needed. For ease of manufacturing, this invention discloses that the chip body 101 is cylindrical, and correspondingly, the inner cavity of the chip body 101 is cylindrical. It is understood that the shape of the chip body 101 disclosed above is only one specific embodiment of this invention. In practical applications, the chip body 101 can also be set to a square or other regular shape cylinder, or other irregular shape irregular structure, etc.

[0117] A loading electrode 103 is mounted on the chip body 101, with one end extending into the inner cavity of the chip body 101. A connecting hole 101a communicating with the inner cavity of the chip body 101 is formed at the first end of the chip body 101. A nanoporous membrane 102 is encapsulated at the first end of the chip body 101 for contacting the part to be introduced. Specifically, the part to be introduced can be the skin layer 400, etc.

[0118] The control instrument 200 includes an instrument body 201 and an electronic control component 202 installed within the instrument body 201. The instrument body 201 has a holding portion for mounting the microfluidic chip 100, and the output end of the electronic control component 202 extends to the holding portion. The instrument body 201 provides a foundation for mounting the electronic control component 202 and the microfluidic chip 100. Furthermore, the instrument body 201 facilitates the movement of the microfluidic chip 100 by moving the instrument body 201, thereby enabling the introduction of liquid to different positions within the introducing portion.

[0119] When the microfluidic chip 100 is installed in the container, the output terminal of the electronic control component 202 is electrically connected to the loading electrode 103. When the electronic control component 202 is powered, the electronic control component 202, the microfluidic chip 100 and the container to be introduced form a closed loop to drive the charged substance in the liquid to be introduced to be delivered to the container through the connecting hole 101a and the nanoporous membrane 102.

[0120] When using the delivery system 1000 provided by this invention, taking human skin as an example, the liquid to be introduced is loaded into the microfluidic chip 100, and the loaded microfluidic chip 100 is installed in the holding part of the control instrument 200, so that the output end of the electronic control component 202 is electrically connected to the loading electrode 103 of the microfluidic chip 100; then, the nanoporous membrane 102 of the microfluidic chip 100 is brought into contact with the human skin; then, the delivery system 1000 is started, and the desired mode is selected. The electronic control component 202 outputs an electrical signal corresponding to the mode to the loading electrode 103 to drive the charged substances in the liquid to be introduced to be delivered to the part to be introduced through the nanoporous membrane 102. This invention utilizes an electric field to deliver the liquid to be introduced to the human skin. The instantaneous voltage opens the cell membrane of the phospholipid bilayer in the skin cells, and the charged molecules in the liquid to be introduced are transported from the outside to the inside of the cells. The liquid to be introduced can be introduced into the skin and cells without piercing the skin, thus improving the introduction efficiency. This invention avoids skin damage while improving delivery efficiency.

[0121] In some embodiments, such as Figure 3 As shown, the electronic control component 202 includes a power supply line, a circuit board 202a, a first connecting electrode 202b, a second connecting electrode 202c, and an external electrode 202d.

[0122] Specifically, the circuit board 202a integrates a control module, which stores multiple preset modes. The parameters corresponding to each mode include pulse voltage, pulse width, pulse interval, and pulse count. These modes include, but are not limited to, whitening, acne treatment, moisturizing, absorption, and anti-aging. Furthermore, it should be noted that each mode can be divided into preset intensity levels, each with a different strength. Adjustment of each intensity level includes adjusting the pulse voltage, pulse width, pulse interval, and pulse count. For example, a mode may have five intensity levels. To facilitate the display of various modes, the circuit board 202a is communicatively connected to a display screen 202a-1, the display surface of which is exposed on the instrument body 201. For easier human-computer interaction, the display screen 202a-1 can also be configured as a touchscreen, allowing switching between different modes via touchscreen operation.

[0123] The power supply line is electrically connected to the circuit board 202a, the first connecting electrode 202b and the second connecting electrode 202c respectively. Specifically, the power supply line can be directly connected to an external power source. Alternatively, a battery 202e can be installed directly inside the instrument body 201 to supply power to the power supply line. The specific method of power supply is not limited.

[0124] The external electrode 202d is arranged around the microfluidic chip 100 in a circumferential manner. The number of the first connecting electrode 202b and the second connecting electrode 202c is at least one. When the microfluidic chip 100 is installed in the holding part, the first connecting electrode 202b is electrically connected to the loading electrode 103, and the second connecting electrode 202c is electrically connected to the external electrode 202d.

[0125] To improve the uniformity of charge distribution of the liquid to be introduced into the microfluidic chip 100, this invention discloses that the centerline of the first connecting electrode 202b coincides with the centerline of the microfluidic chip 100. Understandably, taking a cylindrical shape as an example, the centerline of the microfluidic chip 100 refers to its axis.

[0126] Furthermore, the present invention discloses that the instrument body 201 includes a mounting shell 201a, an external electrode mounting base 201b, a connecting electrode mounting base 201c, and a loading and unloading device 201d.

[0127] The mounting housing 201a has an inner cavity, and the power supply lines and circuit board 202a are all installed in the inner cavity of the mounting housing 201a. A mounting cylinder 201a-1 communicating with the inner cavity of the mounting housing 201a is provided on the mounting housing 201a. For ease of molding, the mounting cylinder 201a-1 can be integrally molded and connected to the mounting housing 201a. Alternatively, other connection methods can be used between the mounting cylinder 201a-1 and the mounting housing 201a.

[0128] The connecting electrode mounting base 201c is installed inside the mounting cylinder 201a-1, and the holding part is a holding groove 201c-1 formed inside the connecting electrode mounting base 201c. The outer electrode mounting base 201b is installed inside the mounting cylinder 201a-1 and is sleeved on the outside of the connecting electrode mounting base 201c. It should be noted that the connection method between the connecting electrode mounting base 201c, the outer electrode mounting base 201b, and the mounting cylinder 201a-1 is not limited, as long as the connection structure can be installed inside the mounting cylinder 201a-1, it is within the protection scope of this invention.

[0129] The external electrode 202d is mounted on the external electrode mounting base 201b and sleeved on the connecting electrode mounting base 201c, and is arranged around the holding groove 201c-1.

[0130] Both the first connecting electrode 202b and the second connecting electrode 202c are mounted on the connecting electrode mounting base 201c. The first connecting electrode 202b extends into the holding tank 201c-1. When the microfluidic chip 100 is installed in the holding tank 201c-1, it abuts against the loading electrode 103 to achieve electrical connection. The second connecting electrode 202c is used to abut against the external electrode 202d to achieve electrical connection.

[0131] The loading / unloading device 201d is installed inside the mounting housing 201a and is used to lock or unlock the microfluidic chip 100 when it is placed in the holding slot 201c-1. The loading / unloading device 201d improves the connection stability between the microfluidic chip 100 and the instrument body 201.

[0132] When the delivery system 1000 can be used for hand-held delivery, an arc-shaped groove that is easy to grip can be provided on the outside of the mounting shell 201a. Alternatively, an anti-slip layer or an anti-slip pad can be attached to the hand-held part of the mounting shell 201a.

[0133] Furthermore, this invention discloses that the angle between the axis of the mounting cylinder 201a-1 and the mounting shell 201a is greater than 90° and less than 180°, facilitating the insertion of the component by hand using the mounting shell 201a. It is understood that setting the angle range between the axis of the mounting cylinder 201a-1 and the mounting shell 201a is only one specific embodiment of this invention. In practical applications, the angle between the axis of the mounting cylinder 201a-1 and the mounting shell 201a may also be set to a value outside this range.

[0134] Furthermore, such as Figure 5 and Figure 6As shown, the present invention discloses a connecting electrode mounting base 201c including a first mounting platform 201c-2, a second mounting platform 201c-3 and a holding cylinder 201c-4. Specifically, the bottom end of the holding cylinder 201c-4 is closed, and the inner cavity of the holding cylinder 201c-4 forms a holding groove 201c-1.

[0135] The container 201c-4 is mounted on the second mounting platform 201c-3, and the second mounting platform 201c-3 is mounted on the first mounting platform 201c-2. Specifically, the container 201c-4, the second mounting platform 201c-3, and the first mounting platform 201c-2 are integrally formed and connected, which improves the overall strength of the connecting electrode mounting base 201c.

[0136] Along the direction from the bottom to the top of the mounting cylinder 201a-1, the mounting cylinder 201a-1 is provided with a first limiting step 201a-1a and a second limiting step 201a-1b at intervals. The end face of the first mounting platform 201c-2 facing the second mounting platform 201c-3 abuts against the step surface of the first limiting step 201a-1a and is fastened together by fasteners, specifically fastening screws, etc., for easy disassembly and assembly.

[0137] The outer electrode mounting base 201b is annular, and its top end abuts against the step surface of the second limiting step 201a-1b. The inner wall of the outer electrode mounting base 201b is provided with an internal thread, and the outer wall of the outer electrode 202d is provided with an external thread that connects with the internal thread of the outer electrode mounting base 201b, so as to facilitate the disassembly and replacement of the outer electrode 202d.

[0138] The second mounting platform 201c-3 has a second electrode mounting hole for mounting the second connecting electrode 202c. The second connecting electrode 202c abuts against the threaded connection between the outer electrode mounting base 201b and the outer electrode 202d. On the one hand, the second connecting electrode 202c achieves electrical connection with the outer electrode 202d. On the other hand, it abuts the outer electrode mounting base 201b against the second limiting step 201a-1b. That is, the installation of the outer electrode mounting base 201b is achieved with the cooperation of the second limiting step 201a-1b, which facilitates the positioning of the outer electrode mounting base 201b.

[0139] The first connecting electrode 202b is installed inside the container 201c-4. Specifically, a mounting platform is provided inside the container 201c-4, and a first electrode mounting hole is opened on the mounting platform. The first connecting electrode 202b is fixed in the first electrode mounting hole. The mounting platform and the container 201c-4 are integrally formed and connected, which facilitates processing and manufacturing.

[0140] Furthermore, this invention discloses that the first connecting electrodes 202b are all elastic electrodes. On the one hand, this ensures stable contact with the carrier electrode of the microfluidic chip 100; on the other hand, the elastic electrodes provide a better soft contact experience, and the entire loading process of the microfluidic chip 100 can be completed with just one press. In addition, when the loading / unloading device 201d unlocks the microfluidic chip 100, the microfluidic chip 100 can be ejected under the elastic restoring force of the first connecting electrodes 202b, facilitating the unloading of the microfluidic chip 100.

[0141] Furthermore, this invention discloses that the second connecting electrode 202c is an elastic electrode. Specifically, there are at least two second connecting electrodes 202c, which are evenly distributed around the axis of the second mounting platform 201c-3. The second connecting electrode 202c being configured as an elastic electrode ensures stable connection between the outer electrode 202d and the second connecting electrode 202c.

[0142] Furthermore, the present invention discloses that, along the axial direction of the mounting cylinder 201a-1, the inner wall of the mounting cylinder 201a-1 and the outer wall of the outer electrode mounting base 201b are provided with a first guide groove 201b-1 on one side and a first guide protrusion that slides in cooperation with the first guide groove 201b-1 on the other side. The provision of the first guide protrusion and the first guide groove 201b-1 effectively avoids the problem that when the outer electrode 202d is screwed onto the outer electrode mounting base 201b, the outer electrode mounting base 201b rotates along with it, causing inconvenience in installing the outer electrode 202d.

[0143] In this embodiment, taking the first guide protrusion provided on the inner wall of the mounting cylinder 201a-1 and the first guide groove 201b-1 opened on the outer electrode mounting seat 201b as an example, in order to facilitate the connection of the electrode mounting seat 201c to be installed into the mounting cylinder 201a-1, the present invention discloses that the second mounting platform 201c-3 is provided with a sliding groove that slides in cooperation with the first guide protrusion.

[0144] Furthermore, the present invention discloses that the inner wall of the container 201c-4 and the outer wall of the chip body 101 are provided with a second guide groove 101b on one side and a second guide protrusion 201c-4a that slides with the second guide groove 101b on the other side, so as to facilitate the installation of the chip body 101 into place.

[0145] In some embodiments, such as Figures 7-9 As shown, the loading and unloading device 201d includes a sleeve 201d-1, a guide shaft 201d-2, a locking member 201d-3, an elastic reset member 201d-4, and a drive assembly 201d-5.

[0146] The sleeve 201d-1 is fixed inside the mounting shell 201a and is open at both ends. Specifically, in order to facilitate the disassembly and assembly of the sleeve 201d-1, the present invention discloses that the sleeve 201d-1 can be detachably installed inside the mounting shell 201a by means of fasteners such as bolts. Sleeve 201d-1 includes a first sleeve 201d-1 and a second sleeve 201d-1. The first sleeve 201d-1 and the second sleeve 201d-1 are connected as a whole by threads. Taking the outer wall of the first sleeve 201d-1 as having an external thread and the inner wall of the second sleeve 201d-1 having an internal thread as an example, the internal thread of the second sleeve 201d-1 is threadedly engaged with the external thread of the first sleeve 201d-1. The outer diameter of the part of the first sleeve 201d-1 with the external thread is smaller than the outer diameter of the first sleeve 201d-1, forming a limiting platform that abuts against the end of the second sleeve 201d-1, which facilitates installation. Furthermore, the outer diameters of the first sleeve 201d-1 and the second sleeve 201d-1 are equal, and the inner diameters of the first sleeve 201d-1 and the second sleeve 201d-1 are also equal, ensuring the flatness of the overall appearance of sleeve 201d-1.

[0147] The guide shaft 201d-2 is fitted inside the sleeve 201d-1. The locking member 201d-3 includes a locking seat 201d-3a and a locking rod 201d-3b connected to the locking seat 201d-3a. The locking seat 201d-3a is slidably fitted onto the outside of the guide shaft 201d-2, and one end abuts against the limiting boss 201d-2a provided on the guide shaft 201d-2. The sleeve 201d-1 is provided with a first sliding through hole. The locking rod 201d-3b extends out of the first sliding through hole and into the holding groove 201c-1, so as to lock the microfluidic chip 100 through the locking part 201d-3c on the locking rod 201d-3b. Correspondingly, the bottom end of the container 201c-4 is provided with an elongated hole that allows the locking rod 201d-3b to pass through, so that the locking rod 201d-3b can drive the locking part 201d-3c to move away from or towards the carrier mechanism.

[0148] Specifically, the locking part 201d-3c is a hook provided on the locking rod 201d-3b, and the carrier mechanism is provided with a groove 101c that engages with the hook. The locking of the carrier mechanism is achieved by the cooperation between the hook and the groove 101c. It can be understood that the structure of the locking part 201d-3c disclosed above is only one specific embodiment of the present invention. In practical applications, the locking part 201d-3c can also be provided as a groove 101c provided on the locking rod 201d-3b, and the carrier structure can be provided with a hook or a hooking protrusion 101g-1b for engaging with the groove 101c.

[0149] The elastic reset member 201d-4 is sleeved outside the guide shaft 201d-2. The first end of the elastic reset member 201d-4 abuts against the end face of the locking seat 201d-3a away from the limiting boss 201d-2a. To facilitate the installation of the elastic reset member 201d-4, this invention discloses that a receiving groove for the first end of the limiting elastic reset member 201d-4 is formed on the end face of the locking seat 201d-3a away from the limiting boss 201d-2a. It should be noted that the locking seat 201d-3a can also be a flat plate or other structures, and is not limited to having a receiving groove.

[0150] The second end of the elastic reset member 201d-4 abuts against the limiting member 201d-2b installed on the guide shaft 201d-2. Specifically, the elastic reset member 201d-4 is a spring in a compressed state. The limiting protrusion can be a protrusion integrally formed with the guide shaft 201d-2, or a protrusion snapped or welded to the guide shaft 201d-2, etc. The protrusion can be an annular protrusion, or a protrusion arranged circumferentially and at intervals around the guide shaft 201d-2, etc. Any structure that can limit the limiting protrusion of the elastic reset member 201d-4 falls within the protection scope of this invention.

[0151] The limiting component 201d-2b is a retaining ring, and an annular groove for installing the retaining ring is provided on the guide shaft 201d-2, which facilitates the disassembly and replacement of the limiting component 201d-2b. Of course, the limiting component 201d-2b can also be a protrusion integrally formed with the guide shaft 201d-2, or it can be a protrusion snapped or welded to the guide shaft 201d-2, etc. The protrusion can be an annular protrusion, or it can be a series of protrusions arranged circumferentially and at intervals around the guide shaft 201d-2, etc. As long as the structure can limit the limiting protrusion of the elastic reset component 201d-4, it is within the protection scope of this invention.

[0152] The drive assembly 201d-5 is slidably installed inside the sleeve 201d-1, and the drive end of the drive assembly 201d-5 is connected to the end of the locking seat 201d-3a facing the limiting boss 201d-2a, so as to drive the locking rod 201d-3b to move away from the microfluidic chip 100, thereby unloading the microfluidic chip 100.

[0153] It should be noted that the drive assembly 201d-5 can be manually driven to slide the locking lever 201d-3b, or it can be electrically driven to slide the locking lever 201d-3b.

[0154] In some embodiments, the number of locking members 201d-3, elastic reset members 201d-4, limiting members 201d-2b, and driving components 201d-5 are all two. The two locking members 201d-3 abut against both ends of the limiting boss 201d-2a, back-to-back. The two limiting members 201d-2b are respectively mounted on the guide shaft 201d-2 and located at both ends of the limiting protrusion. It should be noted that the number of limiting bosses 201d-2a is not limited to one; it can also be two, with the two locking members 201d-3 abutting against the two limiting bosses 201d-2a respectively. In this embodiment, one limiting boss 201d-2a is used as an example for ease of manufacturing. The guide shaft 201d-2 is a cylindrical shaft, and the limiting boss 201d-2a is an annular protrusion. The guide shaft 201d-2 and the limiting boss 201d-2a are integrally formed and connected, which increases the connection strength between the two.

[0155] The first ends of the two elastic reset members 201d-4 abut against the two locking members 201d-3, and the second ends abut against the two limiting members 201d-2b. In other words, the two elastic reset members 201d-4, the two limiting members 201d-2b, and the two locking members 201d-3 achieve force stability for the guide shaft 201d-2, eliminating the need to fix the guide shaft 201d-2 to the sleeve 201d-1. It should be noted that the guide shaft 201d-2 can also be fixed to the sleeve 201d-1. In this case, only one locking member 201d-3, one elastic reset member 201d-4, and one limiting member 201d-2b can be installed on one side of the guide shaft 201d-2 located on the limiting protrusion, while one drive assembly 201d-5 can be installed on the other side of the guide shaft 201d-2 located on the limiting protrusion.

[0156] Two drive components 201d-5 are located at opposite ends of the guide shaft 201d-2. The drive end of any one drive component 201d-5 can slide through the locking seat 201d-3a closest to it and connect with the locking seat 201d-3a furthest from it. This sliding passage of the drive end of any one drive component 201d-5 through the locking seat 201d-3a stabilizes the drive end of the drive component 201d-5 and provides guidance for its sliding movement.

[0157] In some embodiments, the drive assembly 201d-5 includes a drive member 201d-5a, which includes a drive seat 201d-5a-1 and a drive rod 201d-5a-2. The drive seat 201d-5a-1 is slidably mounted inside the sleeve 201d-1. One end of the drive rod 201d-5a-2 is fixed to the drive seat 201d-5a-1, and the other end of the drive rod 201d-5a-2 can slide through a locking seat 201d-3a near the drive seat 201d-5a-1 and connect to the locking seat 201d-3a away from the drive seat 201d-5a-1.

[0158] like Figure 9 As shown, each drive member 201d-5a has two drive rods 201d-5a-2. It should be noted that the number of drive rods 201d-5a-2 on the drive member 201d-5a is not limited to two, and can be any other number, as long as the number of drive rods 201d-5a-2 that can achieve guidance is within the protection scope of this invention.

[0159] The drive rod 201d-5a-2 is a cylindrical rod. One end of the drive rod 201d-5a-2 is connected to the drive seat 201d-5a-1, and the other end is used to pass through the locking seat 201d-3a near the drive seat 201d-5a-1 and connect to the locking seat 201d-3a away from the drive seat 201d-5a-1.

[0160] Furthermore, the present invention discloses that the drive assembly 201d-5 also includes a button 201d-5b, which is installed on the end of the drive seat 201d-5a-1 away from the drive rod 201d-5a-2. The button 201d-5b is exposed outside the sleeve 201d-1, making it easy to press the button 201d-5b.

[0161] Furthermore, the present invention discloses that the outer wall of the button 201d-5b is provided with an abutting protrusion that abuts against the end face of the sleeve 201d-1, so as to avoid over-pressing the button 201d-5b.

[0162] To facilitate the smooth sliding of the drive rod 201d-5a-2 along the first sliding through hole, this invention discloses that the outer wall of the drive base 201d-5a-1 and the inner wall of the sleeve 201d-1 are provided with a guide block on one side and a guide groove that slides with the guide block on the other side. Simultaneously, the guide block and guide groove prevent the drive member 201d-5a from rotating along the axis of the guide shaft 201d-2.

[0163] It is understood that the rotation limit and guidance of the drive component 201d-5a are not limited to the above methods. Alternatively, one side of the button 201d-5b can be set to be flat and slide in contact with the shell wall of the mounting shell 201a.

[0164] Furthermore, the present invention discloses that the drive assembly 201d-5 also includes a magnet block 201d-5c, and the button 201d-5b has an installation groove. The magnet block 201d-5c is installed in the installation groove and is connected to the drive seat 201d-5a-1 by adsorption. The button 201d-5b and the drive seat 201d-5a-1 are connected together by the adsorption of the magnet block 201d-5c, which facilitates the installation and removal of the button 201d-5b.

[0165] In some embodiments, the mounting housing 201a includes a front housing 201a-2, a rear housing 201a-3, and a middle housing 201a-4. The rear housing 201a-3 has support grooves 201a-3a for support sleeves 201d-1 on both side walls. The inner cavity of the rear housing 201a-3 is provided with a plurality of spaced first support plates 201a-3b for supporting the battery 202e of the power supply line, thereby reducing the contact area with the battery 202e and facilitating heat dissipation of the battery 202e.

[0166] The middle shell 201a-4 is installed on the top of the rear shell 201a-3, and the end of the middle shell 201a-4 facing the rear shell 201a-3 is provided with a receiving groove for receiving the sleeve 201d-1, which is recessed in the direction away from the rear shell 201a-3. The receiving groove is also provided with a second sliding through hole that allows the locking rod 201d-3b to pass through and slide. The top of the middle shell 201a-4 is provided with a terminal post 201a-4a for connecting the circuit board 202a. The circuit board 202a is fixed to the terminal post 201a-4a by fastening screws or the like. Of course, a buckle or the like can also be provided on the terminal post 201a-4a to snap the circuit board 202a.

[0167] The front shell 201a-2 covers the top of the middle shell 201a-4, and a display port 201a-2a is provided on the front shell 201a-2. A display screen 202a-1 is communicatively connected to the circuit board 202a. The display screen 202a-1 is installed on the end of the front shell 201a-2 facing the middle shell 201a-4, and the display screen 202a-1 is directly opposite the display port 201a-2a, making it convenient for users to obtain information in a timely manner. In order to protect the display screen 202a-1, this invention discloses that a transparent protective plate 201a-2c for protecting the display screen 202a-1 is also installed on the front shell 201a-2.

[0168] The front cover 201a-2 is also provided with a mechanical button 201a-2b or an electronic button that communicates with the circuit board 202a to switch the output mode to the power supply line output mode. The mounting cylinder 201a-1 is provided on the front cover 201a-2.

[0169] In some embodiments, such as Figures 10-13 As shown, the second end of the chip body 101 has a sample inlet 101d that communicates with the inner cavity for injecting the liquid to be introduced. It should be noted that the sample inlet 101d can be a circular hole or a square hole, etc., and the specific shape is not limited. For ease of processing, this invention discloses that the sample inlet 101d is a circular hole.

[0170] The sample filling cap 104 seals the sample filling port 101d. It should be noted that the sample filling cap 104 is detachably installed at the sample filling port 101d. Specifically, the sample filling cap 104 can be made of elastic rubber or other materials, with a certain elastic deformation capability, so as to achieve a tight fit with the sample filling port 101d and avoid leakage of the liquid to be introduced into the inner cavity.

[0171] To further improve the sealing performance of the sample filling cap 104 and the sample filling port 101d, the present invention discloses that the sample filling port 101d includes a constant diameter hole and a gradually expanding hole. One end of the constant diameter hole is connected to the smaller end of the gradually expanding hole, and the other end of the constant diameter hole is connected to the inner cavity. The larger end of the gradually expanding hole is located at the end face of the second end of the chip body 101. The wall surface of the gradually expanding hole abuts against the sample filling cap 104, increasing the area of ​​the sealing surface and thus improving the sealing performance of the sample filling cap 104 and the sample filling port 101d.

[0172] To avoid the problem of inconvenience in adding liquid caused by gas mixed in the inner cavity of the chip body 101, the present invention discloses that the second end of the chip body 101 is also provided with an exhaust port 101e that communicates with the inner cavity of the chip body 101. The exhaust port 101e is encapsulated with a waterproof and breathable membrane 105 to prevent liquid entering the inner cavity of the chip body 101 from overflowing along the exhaust port.

[0173] Furthermore, this invention discloses that the exhaust port 101e is a countersunk hole, and the waterproof and breathable membrane 105 is ultrasonically bonded to the countersunk hole. It should be noted that the connection between the waterproof and breathable membrane 105 and the countersunk hole is not limited to ultrasonic bonding; welding or bonding are also possible methods. The specific connection method is not limited, as long as it enables the waterproof and breathable membrane 105 to be fixed within the countersunk hole, it falls within the protection scope of this invention.

[0174] In some embodiments, the present invention specifically discloses a chip body 101 including a cavity 101f and a second support plate 101g. One end of the cavity 101f is closed, forming the second end of the chip body 101. The other end of the cavity 101f is open, forming the first end of the chip body 101. The second support plate 101g blocks the open end of the cavity 101f, and a connecting hole 101a is formed on the second support plate 101g. The present invention divides the chip body 101 into a cavity 101f and a support plate, reducing the processing difficulty and facilitating mass production.

[0175] The nanoporous membrane 102 is fixed to the open end face of the cavity 101f and has a preset gap with the second support plate 101g. It should be noted that the preset gap is set according to specific needs and is not limited to a particular value. By setting the preset gap, the liquid to be introduced from the connecting hole 101a can be evenly distributed on the nanoporous membrane 102, facilitating the uniform introduction of the liquid into the insertion section by the nanoporous membrane 102.

[0176] Specifically, the nanoporous membrane 102 is laser-welded to the chip body 101. There are multiple connecting holes 101a, which are evenly distributed on the second support plate 101g to facilitate the uniform entry of the liquid to be introduced between the second support plate 101g and the nanoporous membrane 102.

[0177] Furthermore, the present invention discloses that the inner wall of the open end of the cavity 101f is provided with a third limiting step 101f-1 for limiting and abutting against the end face of the second support plate 101g, which facilitates the installation of the second support plate 101g. In addition, the abutment between the third limiting step 101f-1 and the second support plate 101g increases the contact area between the two, further improving the sealing effect.

[0178] The end of the second support plate 101g facing the cavity 101f is provided with a connecting part 101g-1 for detachable connection with the inner wall of the cavity 101f, which facilitates disassembly and replacement. It should be noted that the detachable connection between the second support plate 101g and the cavity 101f via the connecting part 101g-1 is only one specific embodiment of the present invention. In practical applications, the second support plate 101g and the third limiting step 101f-1 can also be laser welded.

[0179] Taking the detachable connection between the second support plate 101g and the cavity 101f via the connecting part 101g-1 as an example, specifically, the connecting part 101g-1 includes a connecting plate 101g-1a and a snap-fit ​​protrusion 101g-1b disposed on the connecting plate 101g-1a. The connecting plate 101g-1a is fixed on the second support plate 101g, and the inner wall of the cavity 101f is provided with a snap-fit ​​groove 101f-2 that snaps into the snap-fit ​​protrusion 101g-1b. In order to achieve a stable connection between the second support plate 101g and the cavity 101f, the present invention discloses that the number of connecting parts 101g-1 is at least two, and they are evenly distributed in a ring on the second support plate 101g. Of course, the connecting part 101g-1 may include a connecting plate 101g-1a and a snap-fit ​​groove 101f-2 formed on the connecting plate 101g-1a, and the inner wall of the cavity 101f is provided with a snap-fit ​​protrusion 101g-1b for snapping with the snap-fit ​​groove 101f-2.

[0180] Furthermore, the present invention discloses that the shapes of the snap-fit ​​protrusion 101g-1b and the snap-fit ​​groove 101f-2 are matched. Specifically, the snap-fit ​​protrusion 101g-1b is an arc-shaped protrusion, and correspondingly, the snap-fit ​​groove 101f-2 is an arc-shaped groove.

[0181] The detachable connection between the connecting part 101g-1 and the cavity 101f disclosed above is only one specific embodiment of the present invention. In practical applications, other connection methods may also be used. For example, the connecting part 101g-1 is a circular plate fixed on the second support plate 101g, and the circular plate is provided with external threads. The cavity 101f is a cylindrical cavity, and the inner wall of the cavity 101f is provided with internal threads that mate with the external threads.

[0182] Furthermore, this invention discloses that the side wall of the second support plate 101g is provided with a limited rotation guide groove 101g-2, and the inner wall of the cavity 101f is provided with a limited rotation guide protrusion 101f-3 located within the limited rotation guide groove 101g-2. By providing the limited rotation guide protrusion 101f-3 to cooperate with the limited rotation guide groove 101g-2, this invention facilitates the engagement of the second support plate 101g's engaging protrusion 101g-1b into the engaging groove 101f-2 on the inner wall of the cavity 101f, and prevents the second support plate 101g from rotating relative to the cavity 101f, thereby further improving the engagement stability between the second support plate 101g and the cavity 101f.

[0183] Furthermore, the present invention discloses that a guide plate 101g-2 is also provided at the end of the second support plate 101g facing the inner cavity. The side of the guide plate 101g-2 facing the inner cavity is flush with the bottom of the guide groove and slides in contact with the guide protrusion. That is, the guide plate 101g-2 is provided to facilitate the alignment of the second support plate 101g and the inner cavity, and to facilitate the guiding installation of the second support plate 101g.

[0184] In some embodiments, the sample application cover 104 includes a fastening cover 104a, an elastic cover 104b, and a connector 104c. The two ends of the connector 104c are respectively connected to the fastening cover 104a and the elastic cover 104b. A fastening hole 101f-5 is provided on the chip body 101, and the fastening cover 104a is installed within the fastening hole 101f-5. The elastic cover 104b is detachably and sealed within the sample application port 101d. In use, sample application can be performed simply by pulling the elastic cover 104b out of the sample application port 101d. Since the elastic cover 104b is connected to the fastening cover 104a via the connector 104c, the loss of the elastic cover 104b is avoided. It should be noted that the fastening cover 104a and the connector 104c can be made of the same material as the elastic cover 104b, or they can be different materials, depending on the specific requirements. Of course, the fastening hole 101f-5 can be a blind hole or a through hole. When the fastening hole 101f-5 is a through hole, it can also be used for sample loading.

[0185] It is understood that the specific structure of the sample dispensing cap 104 disclosed above is only one specific embodiment of the present invention. In practical applications, the sample dispensing cap 104 may also be configured to include only one elastic cap. The elastic cap is detachably installed at the sample dispensing port 101d. For easy disassembly and assembly, a pull ring or handle may be provided on the elastic cap.

[0186] In some embodiments, a receiving groove 101f-4 is provided at the first end of the chip body 101. A receiving hole communicating with the inner cavity of the chip body 101 is opened at the bottom end of the receiving groove 101f-4, and the axis of the receiving hole coincides with the center of the chip body 101. The loading electrode 103 includes an electrode platform 103a and an electrode post 103b. The electrode post 103b is perpendicularly connected to the electrode platform 103a, and the end face of the electrode platform 103a facing the electrode post 103b is sealed to the receiving groove 101f-4. The electrode post 103b passes through the receiving hole and extends beyond a predetermined length of the receiving hole. It should be noted that the size of the loading electrode 103 and its position distribution on the microfluidic chip 100 are obtained based on precise simulation and calculation of the electric field. This ensures that the resistance of the entire system is minimized, thereby enabling efficient transport of the charged medium inside the chip body 101, inside the nanoporous membrane 102, and into human skin cells. In use, the electric field parameters are determined by simulation calculation based on the conductivity of different liquids to be introduced. Figure 14 and Figure 15 As shown, Figure 14 This is a simulation result diagram of the internal voltage trend distribution obtained by constructing a chip model 300 of the microfluidic chip 100. Figure 15 The figure shows the simulation results of the current density distribution inside the microfluidic chip 100.

[0187] Furthermore, this invention discloses that the loading electrode 103 is an inert metal electrode, ensuring good conductivity while also ensuring that the metal electrode has good inertness and will not be electrolyzed, resulting in good stability. For example, the loading electrode 103 can be an electrode made of gold.

[0188] A second aspect of the present invention provides a delivery method, comprising:

[0189] Step S1: Provide a delivery system 1000 as described in any of the above embodiments;

[0190] Step S2: Load the liquid to be introduced into the inner cavity of the microfluidic chip 100 of the delivery system 1000, and install the loaded microfluidic chip 100 into the holding part of the control instrument 200 of the delivery system 1000, so that the output end of the electronic control component 202 of the delivery system 1000 is electrically connected to the loading electrode 103 of the microfluidic chip 100.

[0191] Step S3: Contact the nanoporous membrane 102 of the microfluidic chip 100 with the part to be introduced;

[0192] Step S4: Start the delivery system 1000 and select the desired mode. The electrical control component 202 outputs an electrical signal corresponding to the mode to the loading electrode 103, driving the charged substance in the liquid to be introduced to be delivered to the injection section through the nanoporous membrane 102.

[0193] The delivery method provided by this invention, taking human skin as the target delivery site, utilizes the nanochannel structure of the nanoporous membrane 102 to precisely focus an electric field on the cell surface, causing the cell membrane (biomembrane) surface to open a hydrophilic channel with a diameter equivalent to that of the nanopores on the nanoporous membrane 102. Then, the voltage across the nanopores accelerates the entry of drug molecules into the cell via electrophoresis, achieving efficient, safe, precise and controllable delivery and transfection of macromolecules in live cells, and can achieve the corresponding targeting purpose inside the cell.

[0194] It should be noted that the words indicating location in this article, such as top and bottom, are all in the form of "top". Figure 6 The orientation settings in the text are merely for ease of expression and do not have any other specific meaning.

[0195] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0196] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0197] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0198] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to any specific implementation. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A delivery system (1000), characterized in that, Includes a microfluidic chip (100) and a control instrument (200); The microfluidic chip (100) includes a chip body (101), a nanoporous membrane (102), and a loading electrode (103). The chip body (101) has an inner cavity for holding the liquid to be introduced. The loading electrode (103) is mounted on the chip body (101), and one end of the loading electrode (103) extends into the inner cavity of the chip body (101). A connecting hole (101a) is opened at the first end of the chip body (101) to communicate with the inner cavity of the chip body (101). The nanoporous membrane (102) is encapsulated at the first end of the chip body (101) for contacting the part to be introduced. The control instrument (200) includes an instrument body (201) and an electronic control component (202) installed in the instrument body. The instrument body (201) is provided with a holding part for mounting the microfluidic chip (100), and the output end of the electronic control component (202) extends to the position of the holding part. When the delivery system (1000) is used, the liquid to be introduced is loaded into the microfluidic chip (100), and the loaded microfluidic chip (100) is installed in the holding part of the control instrument (200), so that the output end of the electronic control component (202) is electrically connected to the loading electrode (103) of the microfluidic chip (100); The instrument body (201) includes a mounting shell (201a), an external electrode mounting base (201b), a connecting electrode mounting base (201c), and a loading / unloading device (201d); the holding part is a holding slot (201c-1) formed in the connecting electrode mounting base (201c); the loading / unloading device (201d) is installed in the mounting shell (201a) and is used to lock or unlock the microfluidic chip (100) when the microfluidic chip (100) is placed in the holding slot (201c-1). When the microfluidic chip (100) is installed in the container, the output terminal of the electronic control component (202) is electrically connected to the loading electrode (103), and when the electronic control component (202) is powered, the electronic control component (202), the microfluidic chip (100) and the container to be introduced form a closed loop to drive the charged substance in the liquid to be introduced to be delivered to the container through the connecting hole (101a) and the nanoporous membrane (102).

2. The delivery system (1000) as claimed in claim 1, characterized in that, The electronic control component (202) includes a power supply line, a circuit board (202a), a first connecting electrode (202b), a second connecting electrode (202c), and an external electrode (202d). The power supply lines are electrically connected to the circuit board (202a), the first connecting electrode (202b), and the second connecting electrode (202c), respectively. The external electrode (202d) is arranged circumferentially around the microfluidic chip (100). The number of the first connecting electrode (202b) and the second connecting electrode (202c) is at least one. When the microfluidic chip (100) is installed in the holding part, the first connecting electrode (202b) is electrically connected to the loading electrode (103), and the second connecting electrode (202c) is electrically connected to the external electrode (202d).

3. The delivery system (1000) as claimed in claim 2, characterized in that, The power supply line and the circuit board (202a) are both installed in the inner cavity of the mounting shell (201a), and the mounting shell (201a) is provided with a mounting cylinder (201a-1) that communicates with the inner cavity of the mounting shell (201a). The connecting electrode mounting base (201c) is installed inside the mounting cylinder (201a-1); The external electrode mounting base (201b) is installed inside the mounting cylinder (201a-1) and sleeved outside the connecting electrode mounting base (201c); The external electrode (202d) is mounted on the external electrode mounting base (201b) and sleeved outside the connecting electrode mounting base (201c), and is arranged around the holding groove (201c-1); The first connecting electrode (202b) and the second connecting electrode (202c) are both mounted on the connecting electrode mounting base (201c). The first connecting electrode (202b) extends into the holding tank (201c-1) and is used to abut against the loading electrode (103) to achieve electrical connection when the microfluidic chip (100) is installed in the holding tank (201c-1). The second connecting electrode (202c) is used to abut against the external electrode (202d) to achieve electrical connection.

4. The delivery system (1000) as claimed in claim 3, characterized in that, The connecting electrode mounting base (201c) includes a first mounting platform (201c-2), a second mounting platform (201c-3), and a holding cylinder (201c-4) with an inner cavity forming a holding groove (201c-1). The container (201c-4) is mounted on the second mounting platform (201c-3), and the second mounting platform (201c-3) is mounted on the first mounting platform (201c-2); Along the direction from the bottom end to the top end of the mounting cylinder (201a-1), a first limiting step (201a-1a) and a second limiting step (201a-1b) are provided at intervals inside the mounting cylinder (201a-1). The end face of the first mounting platform (201c-2) facing the second mounting platform (201c-3) abuts against the step surface of the first limiting step (201a-1a) and is fastened together by fasteners. The container (201c-4) is provided with an installation platform, and the installation platform is provided with a first electrode installation hole for installing the first connecting electrode (202b); The outer electrode mounting base (201b) is annular, and its top end abuts against the step surface of the second limiting step (201a-1b). The inner wall of the outer electrode mounting base (201b) is provided with an internal thread, and the outer wall of the outer electrode (202d) is provided with an external thread that connects with the internal thread of the outer electrode mounting base (201b). The second mounting platform (201c-3) has a second electrode mounting hole for mounting the second connecting electrode (202c), and the second connecting electrode (202c) abuts against the threaded connection of the outer electrode mounting base (201b) and the outer electrode (202d).

5. The delivery system (1000) as claimed in claim 2, characterized in that, Both the first connecting electrode (202b) and the second connecting electrode (202c) are elastic electrodes; and / or In the inner wall of the mounting cylinder (201a-1) and the outer wall of the outer electrode mounting base (201b), along the axial direction of the mounting cylinder (201a-1), one side is provided with a first guide groove (201b-1), and the other side is provided with a first guide protrusion that slides in cooperation with the first guide groove (201b-1). and / or The inner wall of the container (201c-4) and the outer wall of the chip body (101) are provided with a second guide groove (101b) on one side and a second guide protrusion (201c-4a) that slides with the second guide groove (101b) on the other side.

6. The delivery system (1000) as claimed in claim 3, characterized in that, The loading / unloading device (201d) includes: Sleeve (201d-1), wherein the sleeve (201d-1) is fixed inside the mounting shell (201a) and is open at both ends; Guide shaft (201d-2), which is fitted inside sleeve (201d-1); The locking component (201d-3) includes a locking seat (201d-3a) and a locking rod (201d-3b) connected to the locking seat (201d-3a). The locking seat (201d-3a) is slidably sleeved outside the guide shaft (201d-2), and one end abuts against a limiting boss (201d-2a) provided on the guide shaft (201d-2). A first sliding through hole is provided on the sleeve (201d-1). The locking rod (201d-3b) extends out of the first sliding through hole and into the holding groove (201c-1) to lock the microfluidic chip (100) through the locking part (201d-3c) on the locking rod (201d-3b). An elastic reset member (201d-4) is sleeved outside the guide shaft (201d-2). The first end of the elastic reset member (201d-4) abuts against the end face of the locking seat (201d-3a) away from the limiting boss (201d-2a), and the second end of the elastic reset member (201d-4) abuts against the limiting member (201d-2b) installed on the guide shaft (201d-2). A drive assembly (201d-5) is slidably mounted inside the sleeve (201d-1), and the drive end of the drive assembly (201d-5) is connected to the end of the locking seat (201d-3a) facing the limiting boss (201d-2a) to drive the locking rod (201d-3b) to move away from the microfluidic chip (100), thereby unloading the microfluidic chip (100).

7. The delivery system (1000) as claimed in claim 6, characterized in that, The number of the locking member (201d-3), the elastic reset member (201d-4), the limiting member (201d-2b), and the driving assembly (201d-5) is two each; The two locking members (201d-3) abut against the two ends of the limiting boss (201d-2a) back to back, and the two limiting members (201d-2b) are respectively mounted on the guide shaft (201d-2) and are respectively located at the two ends of the limiting protrusion; The first ends of the two elastic reset members (201d-4) abut against the two locking members (201d-3) respectively, and the second ends abut against the two limiting members (201d-2b) respectively. The two drive components (201d-5) are located at both ends of the guide shaft (201d-2), and the drive end of any one of the drive components (201d-5) can slide through the locking seat (201d-3a) near the drive component (201d-5) and connect to the locking seat (201d-3a) away from the drive component (201d-5).

8. The delivery system (1000) as claimed in claim 6, characterized in that, The drive assembly (201d-5) includes a drive element (201d-5a); The driving component (201d-5a) includes a driving seat (201d-5a-1) and a driving rod (201d-5a-2). The driving seat (201d-5a-1) is slidably installed inside the sleeve (201d-1). One end of the driving rod (201d-5a-2) is fixed on the driving seat (201d-5a-1), and the other end of the driving rod (201d-5a-2) can slide through a locking seat (201d-3a) near the driving seat (201d-5a-1) and connect to a locking seat (201d-3a) away from the driving seat (201d-5a-1).

9. The delivery system (1000) as claimed in claim 8, characterized in that, The drive assembly (201d-5) also includes a button (201d-5b); The button (201d-5b) is installed on the end of the drive seat (201d-5a-1) away from the drive rod (201d-5a-2), and the button (201d-5b) is exposed on the sleeve (201d-1).

10. The delivery system (1000) as claimed in claim 9, characterized in that, The drive assembly (201d-5) also includes a magnet (201d-5c). The button (201d-5b) has a mounting groove. The magnet (201d-5c) is attached to the mounting groove and is attached to the drive base (201d-5a-1). and / or The locking part (201d-3c) is a hook provided on the locking rod (201d-3b), and the chip body (101) is provided with a slot (101c) that engages with the hook. and / or The locking seat (201d-3a) has a receiving groove on the end face opposite to the limiting boss (201d-2a) to limit the first end of the elastic reset member (201d-4).

11. The delivery system (1000) as described in any one of claims 6-10, characterized in that, The mounting housing (201a) includes a front housing (201a-2), a rear housing (201a-3), and a middle housing (201a-4). The rear shell (201a-3) has support grooves (201a-3a) on both side walls to support the sleeve (201d-1), and the inner cavity of the rear shell (201a-3) is provided with a plurality of spaced first support plates (201a-3b) to support the battery (202e) of the power supply line. The middle shell (201a-4) covers the top of the rear shell (201a-3), and the end of the middle shell (201a-4) facing the rear shell (201a-3) is provided with a receiving groove that is recessed in the direction away from the rear shell (201a-3) to accommodate the sleeve (201d-1). The receiving groove is also provided with a second sliding through hole that allows the locking rod (201d-3b) of the delivery system to pass through and slide. The top of the middle shell (201a-4) is provided with a terminal (201a-4a) for connecting the circuit board (202a). The front shell (201a-2) covers the top of the middle shell (201a-4), and the front shell (201a-2) has a display port (201a-2a). The circuit board (202a) is communicatively connected to a display screen (202a-1). The display screen (202a-1) is installed on the end of the front shell (201a-2) facing the middle shell (201a-4), and the display screen (202a-1) is directly opposite the display port (201a-2a). The front shell (201a-2) is also provided with a mechanical button (201a-2b) or an electronic button that is communicatively connected to the circuit board (202a) to switch the output mode of the power supply line. The mounting cylinder (201a-1) is disposed on the front shell (201a-2).

12. The delivery system (1000) as claimed in claim 1, characterized in that, The second end of the chip body (101) is provided with a sample inlet (101d) that communicates with the inner cavity of the chip body (101) to inject the liquid to be introduced. The sample inlet (101d) is sealed with a sample cap (104). The second end of the chip body (101) is also provided with an exhaust port (101e) that communicates with the inner cavity of the chip body (101), and the exhaust port (101e) is encapsulated with a waterproof and breathable membrane (105). and / or The chip body (101) includes a cavity (101f) and a second support plate (101g). One end of the cavity (101f) is closed, which is the second end of the chip body (101); The other end of the cavity (101f) is open and is the first end of the chip body (101). The second support plate (101g) blocks the open end of the cavity (101f), and the connecting hole (101a) is formed on the second support plate (101g). The nanoporous membrane (102) is fixed on the end face of the open end of the cavity (101f) and has a preset gap with the second support plate (101g).

13. The delivery system (1000) as claimed in claim 12, characterized in that, The number of the connecting holes (101a) is multiple, and they are evenly distributed on the second support plate (101g); and / or The inner wall of the open end of the cavity (101f) is provided with a third limiting step (101f-1) for limiting and abutting against the end face of the second support plate (101g). The second support plate (101g) is provided with a connecting part (101g-1) at the end facing the cavity (101f) so as to be detachably connected to the inner wall of the cavity (101f); or, the second support plate (101g) and the third limiting step (101f-1) are laser welded together.

14. The delivery system (1000) as claimed in claim 13, characterized in that, The connecting part (101g-1) includes a connecting plate (101g-1a) and a snap-fit ​​protrusion (101g-1b) provided on the connecting plate (101g-1a). The connecting plate (101g-1a) is fixed on the second support plate (101g). The inner wall of the cavity (101f) is provided with a snap-fit ​​groove (101f-2) that snaps into the snap-fit ​​protrusion (101g-1b). or The connecting part (101g-1) includes a connecting plate (101g-1a) and a snap-fit ​​groove (101f-2) formed on the connecting plate (101g-1a). The inner wall of the cavity (101f) is provided with a snap-fit ​​protrusion (101g-1b) for snapping with the snap-fit ​​groove (101f-2). or The connecting part (101g-1) is a circular ring plate fixed on the second support plate (101g), and the circular ring plate is provided with external threads. The cavity (101f) is a cylindrical cavity, and the inner wall of the cavity (101f) is provided with internal threads that mate with the external threads. and / or The second support plate (101g) has a limited rotation guide groove (101g-2) on its side wall, and the cavity (101f) has a limited rotation guide protrusion (101f-3) located in the limited rotation guide groove (101g-2) on its inner wall. and / or The sample dispensing cap (104) includes a fastening cap (104a), an elastic cap (104b), and a connector (104c). The two ends of the connector (104c) are respectively connected to the fastening cap (104a) and the elastic cap (104b). The chip body (101) has a fastening hole (101f-5). The fastening cap (104a) is installed in the fastening hole (101f-5), and the elastic cap (104b) is detachably and sealed in the sample dispensing port (101d). and / or The waterproof and breathable membrane (105) is connected to the chip body (101) by ultrasonic bonding; and / or The loading electrode (103) is an inert metal electrode; and / or The first end of the chip body (101) is provided with a receiving groove (101f-4). The bottom end of the receiving groove (101f-4) is provided with a receiving hole that communicates with the inner cavity of the chip body (101). The axis of the receiving hole coincides with the center of the chip body (101). The loading electrode (103) includes an electrode platform (103a) and an electrode post (103b). The electrode post (103b) is perpendicularly connected to the electrode platform (103a). The end face of the electrode platform (103a) facing the electrode post (103b) is sealed to the receiving groove (101f-4). The electrode post (103b) passes through the receiving hole and extends beyond the predetermined length of the receiving hole. and / or The nanoporous membrane (102) is laser-welded to the chip body (101).

15. A delivery method, characterized in that, include: Provide a delivery system as described in any one of claims 1-14; The liquid to be introduced is loaded into the cavity of the microfluidic chip (100) of the delivery system, and the loaded microfluidic chip (100) is installed in the holding part of the control instrument (200) of the delivery system, so that the output end of the electronic control component (202) of the delivery system is electrically connected to the loading electrode (103) of the microfluidic chip (100). The nanoporous membrane (102) of the microfluidic chip (100) is brought into contact with the part to be introduced; The delivery system is started and the desired mode is selected. The electrical control component (202) outputs an electrical signal corresponding to the mode to the loading electrode (103) to drive the charged substance in the liquid to be introduced to be delivered to the part to be introduced through the nanoporous membrane (102).

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