A high-dispersion lens assembly, design method, and biological detection system
By designing a high dispersion lens assembly, using the combination of multiple positive and negative lenses and the Amishi prism design, the problem of difficulty in distinguishing excitation light from detecting light in existing lenses is solved, effectively focusing and separation of light at different wavelengths is achieved, and imaging effects of biofluorescence detection and wavelength separation are improved.
Patent Information
- Application Number
- CN202211094417.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-09-08
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2042-09-08
AI Technical Summary
Existing lens designs are difficult to effectively distinguish between excitation light and detection light, especially in biofluorescence detection and wavelength separation applications, resulting in poor detection of light wave signal.
A high dispersion lens assembly is designed, including a receiving element, a relay element and a focusing element. Through the combination of multiple positive lenses and negative lenses, the Arbey coefficient is arranged from large to small, combined with the Amesi prism design principle, the dispersion and focus of light at different wavelengths is achieved, ensuring that light at different wavelengths is focused at different positions in the same focal plane.
Effective separation and focus of light at different wavelengths is achieved, the imaging effect of biofluorescence detection and wavelength separation is improved, the light loss is reduced, and the detection accuracy is enhanced.
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Figure CN115452781B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of lens design, and in particular to a high-dispersion lens assembly, a design method and a biological detection system. Background Art
[0002] To achieve clear imaging, traditional lens design typically aims to focus all wavelengths onto a single point, defined as the optimal focal point with the smallest "circle of confusion." Specifically, lens design employs a variety of lens combinations and lenses made of low-dispersion materials to focus different wavelengths onto the same point.
[0003] However, in applications such as bioluminescence detection and wavelength-separated spectral detection, this is actually detrimental to the detection of optical signals. In some biometric detection applications, a light source of a specific wavelength is used as excitation light to stimulate a bioluminescent reagent to produce detection light, and the energy of this detection light is detected to achieve biorecognition. Staff typically add narrowband filtering between the detection chip and the lens to separate the excitation and detection light. However, when the wavelengths of the excitation and detection lights are too close, existing traditional lens designs have difficulty effectively distinguishing the excitation and detection lights. Summary of the Invention
[0004] The present application provides a high-dispersion lens assembly, a design method, and a biological detection system, which can focus light of different wavelengths at different positions on the same focal plane.
[0005] In a first aspect, the present application provides a high-dispersion lens assembly, comprising: the high-dispersion lens assembly is composed of a receiving element, a relay element, and a focusing element in order from the incident direction of light;
[0006] The receiving element is used to receive the excitation light beam and disperse the excitation light into multiple beams of dispersed light according to wavelength;
[0007] The relay element is used to focus each of the dispersed lights having the same wavelength to generate a relay dispersed light group; wherein the relay element includes a lens group composed of a plurality of positive lenses and negative lenses, and each of the positive lenses is arranged in descending order according to the Abbe coefficient;
[0008] The focusing element is used to focus the focused light spot corresponding to each relay dispersed light group to different positions on the same focal plane; wherein, the focusing element includes at least two lenses with different refractive indices, and the lenses are arranged in order from small to large according to the refractive index.
[0009] In this way, after receiving the excitation light beam, the excitation light beam is dispersed into multiple beams of dispersed light and focused according to the wavelengths of different dispersed lights. The high-dispersion lens assembly provided by the present application contains multiple positive lenses and each lens is arranged from large to small according to the Abbe coefficient. The dispersion effect in the relay element shows a step-by-step enhancement trend, so that after the relay element receives the dispersed light, it can focus according to the wavelengths of different dispersed lights to generate a relay dispersion group. Furthermore, according to the design principle of the Amish prism, it is optimized and two lenses with different refractive indices are arranged from small to large as focusing elements. After refraction by lenses with different refractive indices, the distance between the focusing spots corresponding to each relay dispersion group is widened, thereby achieving the focusing of light of different wavelengths at different positions on the same focal plane.
[0010] In one implementation, the receiving element includes a first receiving lens and a second diverging lens, specifically comprising:
[0011] The first receiving lens is a convex lens, and the second diverging lens is a plano-concave lens;
[0012] The first receiving lens is used to convert the received excitation light beam into parallel light;
[0013] The second diverging lens is used to disperse the parallel light into the dispersed light according to wavelength; wherein the concave surface of the plano-concave lens faces the side of the excitation light beam.
[0014] In one implementation, at least one positive lens in the focusing element is made of polycarbonate material or modified alkane material with high dispersion.
[0015] In one implementation, the relay element includes at least two types of lenses: a biconvex positive lens and a meniscus positive lens.
[0016] In a second aspect, the present application further provides a method for designing a high-dispersion lens, comprising: receiving an excitation light beam and dispersing the excitation light into multiple beams of dispersed light according to wavelength;
[0017] focusing each of the dispersed lights having the same wavelength to generate a relay dispersed light group;
[0018] The focused light spots corresponding to each relay dispersed light group are focused to different positions on the same focal plane.
[0019] In a third aspect, the present application further provides a biological detection system, comprising:
[0020] A laser emitter, an optical filter, a high-dispersion lens, and a detection module; wherein the high-dispersion lens comprises the high-dispersion lens assembly described above, specifically:
[0021] The laser emitter is used to emit stable excitation light to the object to be measured so that the object to be measured generates detection light;
[0022] The optical filter is used to filter the optical signal entering the high-dispersion lens; wherein the optical filter is arranged between the laser emitter and the high-dispersion lens, and the optical signal includes the excitation light and the detection light;
[0023] The high dispersion lens is used to separate the excitation light and the detection light;
[0024] The detection module is used to identify the detection light after passing through the high-dispersion lens to obtain a biometric recognition result of the object to be detected.
[0025] Thus, a high-dispersion lens using the high-dispersion lens assembly described above has excellent high-dispersion capabilities, allowing it to focus light of different wavelengths at different locations on the same focal plane. This application provides a biological detection system using this high-dispersion lens, which is suitable for applications such as biofluorescence detection and wavelength separation.
[0026] In one implementation, the optical biological detection system further includes a reflector, specifically:
[0027] The reflector is arranged between the laser emitter and the high-dispersion lens at a preset angle so that the excitation light beam emitted by the laser emitter is in a perpendicular relationship with the high-dispersion lens.
[0028] A biological detection system provided in the present application also includes a reflector, which is arranged at a preset angle between a laser emitter and a high-dispersion lens to ensure that the excitation light beam emitted by the laser emitter can be vertically absorbed by the high-dispersion lens, thereby reducing the loss of light entering the high-dispersion lens and improving the imaging effect.
[0029] In one implementation, the center of the optical filter coincides with the optical axis of the high-dispersion lens;
[0030] The optical signal includes at least three different colors of visible light, and each of the visible lights is focused on a corresponding light spot;
[0031] After the optical signal passes through the high-dispersion lens, a plurality of focused light spots are generated on a focal plane; wherein the radius of the largest-sized light spot among the focused light spots is at least 1.414 times the radius of the smallest-sized light spot.
[0032] In one implementation, the high-dispersion lens further includes a photoelectric converter and a data converter, specifically:
[0033] The photoelectric converter is used to generate corresponding charges according to the received light signal;
[0034] The data converter is used to read the charge accumulated by the photoelectric converter and convert it into a corresponding digital quantization value, so as to achieve separation of the excitation light and the detection light.
[0035] In one implementation, the detection module is used to identify the detection light after passing through the high-dispersion lens to obtain a biometric recognition result of the object to be detected, specifically:
[0036] The detection module selects a corresponding detection method according to the category of the object to be detected to identify the detection light, so as to obtain a biometric recognition result of the object to be detected.
[0037] In a fourth aspect, the present application also provides a terminal device, comprising a processor, a memory, and a computer program stored in the memory and configured to be executed by the processor, wherein when the processor executes the computer program, the high-dispersion lens assembly design method as described above is implemented.
[0038] In a fifth aspect, the present application further provides a computer-readable storage medium, wherein the computer-readable storage medium includes a stored computer program, wherein when the computer program is executed, the device containing the computer-readable storage medium is controlled to execute the high-dispersion lens assembly design method as described above. BRIEF DESCRIPTION OF THE DRAWINGS
[0039] Figure 1 1 is a module structure diagram of a high-dispersion lens assembly provided by an embodiment of the present invention;
[0040] Figure 2 This is a schematic diagram of the relationship between wavelength and focal length in an existing lens;
[0041] FIG3( a ) is a schematic diagram of the focus shape of a red light spot after passing through an existing lens;
[0042] FIG3( b ) is a schematic diagram of the focus shape of the green light spot after passing through the existing lens;
[0043] FIG3( c ) is a schematic diagram of the focus shape of the blue light spot after passing through the existing lens;
[0044] Figure 4 Schematic diagram of the relationship between wavelength and focal length in a high-dispersion lens assembly provided by an embodiment of the present invention;
[0045] Figure 5(a) is a schematic diagram of the focus shape of the red light spot after passing through a high-dispersion lens;
[0046] Figure 5(b) is a schematic diagram of the focus shape of the green light spot after passing through the high dispersion lens;
[0047] Figure 5(c) is a schematic diagram of the focus shape of the blue light spot after passing through the high dispersion lens;
[0048] Figure 6 1 is a flow chart of a method for designing a high-dispersion lens provided by an embodiment of the present invention;
[0049] Figure 7 This is a module structure diagram of a biological detection system provided by an embodiment of the present invention. DETAILED DESCRIPTION
[0050] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.
[0051] The terms "first," "second," and the like in the specification, claims, and accompanying drawings of this application are used to distinguish between different objects, not to describe a particular order. Furthermore, the terms "including," "having," and any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, product, or apparatus comprising a series of steps or elements is not limited to the listed steps or elements, but may optionally include steps or elements not listed, or may optionally include other steps or elements inherent to the process, method, product, or apparatus.
[0052] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.
[0053] First, some terms in this application are explained to facilitate understanding by those skilled in the art.
[0054] (1) Collimating lens: A collimating lens is an instrument that can transform the light from each point in the aperture column into a parallel collimated beam of light.
[0055] (2) Plano-concave lens: A negative lens with a negative focal length. When a parallel light beam enters a plano-concave lens, it will diverge.
[0056] Example 1
[0057] See also Figure 1 , Figure 1 This is a module structure diagram of a high-dispersion lens assembly provided by an embodiment of the present invention. The embodiment of the present invention provides a high-dispersion lens assembly, which includes a receiving element 101, a relay element 102, and a focusing element 103 in the order of incident light direction;
[0058] The receiving element 101 is used to receive the excitation light beam and disperse the excitation light into multiple dispersed light beams according to the wavelength;
[0059] The relay element 102 is used to focus each of the dispersed lights having the same wavelength to generate a relay dispersed light group. The relay element includes a lens group composed of a plurality of positive lenses and negative lenses. The positive lenses are arranged in descending order of Abbe coefficient.
[0060] The focusing element 103 is used to focus the focus spot corresponding to each relayed dispersed light group to different positions on the same focal plane; wherein the focusing element includes at least two lenses with different refractive indices, and the lenses are arranged in order from small to large according to the refractive index.
[0061] In an embodiment of the present invention, the receiving element 101 includes a first receiving lens and a second diverging lens, specifically including: the first receiving lens is a convex lens, and the second diverging lens is a plano-concave lens; the first receiving lens is used to convert the received excitation light beam into parallel light; the second diverging lens is used to disperse the parallel light into the dispersed light according to the wavelength; wherein the concave side of the plano-concave lens faces the side of the excitation light beam.
[0062] Preferably, in this embodiment, the first receiving lens is a collimating lens, which is used to form the excitation light beam into collimated light or parallel light along the optical axis. Thereby, the received excitation light beam will not diverge with distance, and the different wavelength components contained in the excitation light beam can be collimated into parallel light and emitted to the second emitting lens. The second diverging lens is a plano-concave lens, which is used to diverge the received parallel light into dispersed light. Among them, the plano-concave lens is concave toward the excitation light beam. In order to reduce the interference of stray light in the excitation light beam, the Abbe coefficient Vd of the plano-concave lens in this embodiment can be set to a value between 18≤Vd≤20.
[0063] The relay element 102 includes several positive lenses, and is arranged in order from small to large according to the Abbe coefficient. In an embodiment of the present invention, the relay element 102 uses three meniscus positive lenses to construct a focusing element to focus on the typical wavelength of blue, the typical wavelength of green, and the typical wavelength of red respectively. The typical wavelength of blue light is 435nm~450nm, the typical wavelength of green light is 492nm~577nm, and the typical wavelength of red light is 622nm~760nm. Preferably, the Abbe coefficient Vd of the first meniscus positive lens is in the range of 45≤Vd≤50; the Abbe coefficient Vd of the second meniscus positive lens is in the range of 35≤Vd≤40; and the Abbe coefficient of the third meniscus mirror is in the range of 17≤Vd≤20. A relay element provided by an embodiment of the present invention includes multiple meniscus positive lenses, and each lens is arranged in order from large to small according to the Abbe coefficient. The dispersion effect in the relay element shows a step-by-step enhancement trend, so that after the relay element receives the dispersed light, it can focus according to the wavelength of different dispersed light. In order to further improve the focusing effect of each lens in the relay element, at least one positive lens in the relay element is made of polycarbonate material or modified alkane-based high-dispersion material.
[0064] It should be noted that the relay element can also include more types of positive lenses with different Abbe coefficients. This embodiment of the present invention is merely an example. When the excitation beam contains dispersed light of various wavelengths, lenses with different Abbe coefficients are selected for focusing. To achieve more precise and detailed focusing, multiple lenses with different Abbe coefficients can be configured. A greater number of lenses results in better imaging, but a greater number of lenses increases the axial length, weight, and cost of the high-dispersion lens assembly. This can be flexibly selected based on specific circumstances during implementation, and this embodiment of the present invention does not impose any limitations.
[0065] The focusing element 103 is used to focus the focused light spot corresponding to each relay dispersed light group to different positions on the same focal plane. In an embodiment of the present invention, two lenses with different refractive indices are used in the focusing element to focus the dispersed light groups of different wavelengths passing through the relay element to different positions on the same focal plane. The lens type on the side close to the relay element is set to a double convex positive lens, and the other lens type is set to a meniscus positive lens, and the refractive index of the double convex positive lens is lower than that of the meniscus positive lens, and the air gap between the two lenses is set to 0.1mm. The design principle of the Amish prism is as follows: The Amish prism consists of two triangular prisms, the first triangular prism is usually made of glass with medium dispersion power, and the second is made of glass with higher dispersion power (compared to the first). When the light enters the first prism, it is first refracted, and then enters the interface between the two prisms. Due to the different refractive indices of the two glass materials, it is refracted again at this interface. The focusing element in the embodiments of the present invention is designed based on the principles of Amish prism design. After refraction by lenses of different refractive indices, the distance between the focused light spots corresponding to each relay dispersion group is increased, thereby focusing light of different wavelengths at different locations on the same focal plane. It should be noted that the lens type on the side closest to the relay element can also be set to a positive meniscus lens or other lens types. The design of the focusing element in this application is merely illustrative and does not limit the structure of the focusing element. The number and type of lenses within the focusing element can be adaptively adjusted based on the principles of Amish prism design.
[0066] See also Figure 2 , Figure 2 This is a schematic diagram of the relationship between wavelength and focal length in an existing lens. Figure 2 In the figure, the horizontal axis is the focus and the vertical axis is the wavelength. In order to achieve a clearer imaging effect, the light of different wavelengths is generally focused as close as possible. Specifically, when the diffraction limit of the system is known, the system chromatic aberration is designed to approach the diffraction limit of the system. Figure 2 , it can be seen that the focal positions of light of different wavelengths overlap to a large extent. Preferably, when the system diffraction limit is determined, the existing technology will design the system chromatic aberration to be as close to the diffraction limit as possible, and the specific degree of closeness is determined according to the lens design combination. See Figure 3 (a), Figure 3 (b) and Figure 3 (c), which are schematic diagrams of the focus shapes of the light spots of red light, green light and blue light after passing through the existing lens, respectively. In an embodiment of the present invention, a traditional lens design structure is adopted. It is known that the system diffraction limit is 94μm. After the light to be measured passes through the lens design, its system chromatic aberration is 134μm, among which the red light spot radius is 1.94μm; the green light spot radius is 2.97μm; and the blue light spot radius is 0.87μm. The visible light spots of different wavelengths are basically concentrated in one position.
[0067] See also Figure 4, Figure 4 This is a schematic diagram of the relationship between wavelength and focal length in a high-dispersion lens assembly provided by an embodiment of the present invention. Figure 4 In the figure, the horizontal axis is the focus and the vertical axis is the wavelength. In an embodiment of the present invention, in order to separate different wavelengths, light of different wavelengths is focused at different positions on the same focal plane in the lens design. Specifically, given a known system diffraction limit, a high-dispersion lens assembly provided by an embodiment of the present invention is used to achieve system chromatic aberration away from the system diffraction limit. Different variations in system chromatic aberration can also be achieved by adjusting the number of lenses in the assembly. Given a known system diffraction limit of 139 μm, after the light to be measured passes through a high-dispersion lens assembly provided by the present application, its system chromatic aberration can reach 7610 μm. Referring to Figures 5(a), 5(b), and 5(c), they are schematic diagrams of the spot focus shapes of red light, green light, and blue light after passing through a high-dispersion lens provided by an embodiment of the present invention, respectively. In this embodiment of the present invention, the system diffraction limit is 139 μm and the system chromatic aberration is 7610 μm. The red light spot radius is 211 μm; the green light spot size is 1.4 μm; and the blue light spot size is 88 μm. A high-dispersion lens assembly provided by an embodiment of the present invention can significantly increase the distance between the focal spots of light spots of different wavelengths, and also produce obvious differences in the sizes of the focal spots of light spots of different wavelengths, further achieving the effect of separating different wavelengths.
[0068] An embodiment of the present invention provides a high-dispersion lens assembly, which disperses the excitation light beam into multiple beams of dispersed light after receiving the excitation light beam, and focuses according to the wavelengths of different dispersed lights. The high-dispersion lens assembly provided by the present application includes multiple positive lenses, and each lens is arranged from large to small according to the Abbe coefficient. The dispersion effect in the relay element shows a step-by-step enhancement trend, so that after the relay element receives the dispersed light, it can focus according to the wavelengths of different dispersed lights to generate a relay dispersion group. Furthermore, according to the design principle of the Amish prism, it is optimized and two lenses with different refractive indices are arranged from small to large as focusing elements. After refraction by lenses with different refractive indices, the distance between the focusing spots corresponding to each relay dispersion group is widened, thereby achieving the goal of focusing light of different wavelengths at different positions on the same focal plane.
[0069] Example 2
[0070] See also Figure 6 , Figure 6 2 is a flow chart of a method for designing a high-dispersion lens provided by an embodiment of the present invention, including steps 201 to 203. The details of each step are as follows:
[0071] Step 201: receiving an excitation light beam and dispersing the excitation light into multiple dispersed light beams according to wavelength;
[0072] Step 202: Focusing each of the dispersed lights having the same wavelength to generate a relay dispersed light group;
[0073] Step 203: focusing the focused light spots corresponding to each of the relayed dispersed light groups to different positions on the same focal plane.
[0074] The embodiments of the present invention are applicable to a high-dispersion lens assembly as described in Example 1. Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working process of the high-dispersion lens assembly design method described above can refer to the corresponding working process of the high-dispersion lens assembly in the aforementioned Example 1, and will not be repeated here.
[0075] In an embodiment of the present invention, a device for collecting data based on a high-dispersion lens assembly design device is also provided. The device includes a processor, a memory, and a computer program stored in the memory and configured to be executed by the processor. When the processor executes the computer program, the above-mentioned high-dispersion lens assembly design method is implemented.
[0076] In an embodiment of the present invention, a computer-readable storage medium is also provided. The computer-readable storage medium includes a stored computer program. When the computer program is executed, the device containing the computer-readable storage medium is controlled to execute the above-described high-dispersion lens assembly design method. Exemplarily, the computer program can be divided into one or more modules, which are stored in the memory and executed by the processor to implement the present invention. The one or more modules can be a series of computer program instruction segments capable of performing specific functions, and the instruction segments are used to describe the execution process of the computer program in a device based on data collection of a high-dispersion lens assembly design device.
[0077] The data collection device based on the high-dispersion lens assembly design device can be a computing device such as a desktop computer, laptop, PDA, or cloud server. The data collection device based on the high-dispersion lens assembly design device can include, but is not limited to, a processor, memory, and a display. Those skilled in the art will appreciate that the aforementioned components are merely examples of data collection devices based on the high-dispersion lens assembly design device and do not constitute a limitation on the data collection device based on the high-dispersion lens assembly design device. The device can include more or fewer components than those described above, or a combination of certain components, or different components. For example, the data collection device based on the high-dispersion lens assembly design device can also include input / output devices, network access devices, buses, and the like.
[0078] The processor may be a central processing unit (CPU), other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor may be a microprocessor or any conventional processor. The processor serves as the control center of the device for collecting data based on the high-dispersion lens assembly design device, and utilizes various interfaces and circuits to connect various components of the device for collecting data based on the high-dispersion lens assembly design device.
[0079] The memory can be used to store the computer programs and / or modules. The processor implements the various functions of the device for collecting data based on the high-dispersion lens assembly design device by running or executing the computer programs and / or modules stored in the memory and accessing the data stored in the memory. The memory may primarily include a program storage area and a data storage area. The program storage area may store an operating system and at least one application required for a function (such as a sound playback function or a text conversion function); the data storage area may store data generated based on the use of the mobile phone (such as audio data, text message data, etc.). Furthermore, the memory may include high-speed random access memory and non-volatile memory, such as a hard disk, internal memory, a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash card, at least one disk storage device, a flash memory device, or other volatile solid-state storage device.
[0080] If the module integrated into the device for data collection based on the high-dispersion lens assembly design device is implemented as a software functional unit and sold or used as a standalone product, it can be stored in a computer-readable storage medium. Based on this understanding, the present invention can also implement all or part of the processes in the above-mentioned method embodiments by instructing the relevant hardware through a computer program. The computer program can be stored in a computer-readable storage medium, and when executed by a processor, the computer program can implement the steps of each of the above-mentioned method embodiments. The computer program includes computer program code, which can be in source code form, object code form, executable file, or some intermediate form. The computer-readable medium can include any entity or device capable of carrying the computer program code, a recording medium, a USB flash drive, a removable hard drive, a magnetic disk, an optical disk, a computer memory, a read-only memory (ROM), a random access memory (RAM), an electrical carrier signal, a telecommunications signal, and a software distribution medium. It should be noted that the content of the computer-readable medium may be appropriately increased or decreased based on the requirements of legislation and patent practice in a jurisdiction. For example, in some jurisdictions, based on legislation and patent practice, computer-readable media does not include electric carrier signals and telecommunication signals. Persons of ordinary skill in the art can understand and implement the present invention without inventive effort.
[0081] Embodiments of the present invention provide a high-dispersion lens design method. Unlike existing techniques that focus all wavelengths onto a single point to achieve clear imaging, this method disperses the excitation beam into multiple dispersed beams after receiving them. These beams are then focused according to their wavelengths to generate relay dispersed light groups. The distances between the focused light spots corresponding to each relay dispersed light group are increased, thereby focusing light of different wavelengths onto different locations on the same focal plane. This method is suitable for applications such as bioluminescence detection and wavelength separation.
[0082] Example 3
[0083] See also Figure 7 , Figure 7 is a block diagram of a biological detection system provided by an embodiment of the present invention. This embodiment of the present invention provides a biological detection system comprising a laser emitter 301, an optical filter 302, a high-dispersion lens 303, and a detection module 304. The high-dispersion lens 303 comprises the high-dispersion lens assembly described in Example 1, specifically:
[0084] The laser emitter 301 is used to emit stable excitation light to the object to be measured so that the object to be measured generates detection light;
[0085] The optical filter 302 is used to filter the optical signal entering the high-dispersion lens; wherein the optical filter 302 is arranged between the laser emitter and the high-dispersion lens, and the optical signal includes the excitation light and the detection light;
[0086] The high dispersion lens 303 is used to separate the excitation light and the detection light;
[0087] The detection module 304 is used to identify the detection light after passing through the high-dispersion lens to obtain a biometric recognition result of the object to be detected.
[0088] In this embodiment of the present invention, the bioassay system further includes a reflector. Specifically, the reflector is positioned at a predetermined angle between the laser emitter 301 and the high-dispersion lens 303 to ensure that the excitation beam emitted by the laser emitter 301 is perpendicular to the high-dispersion lens 303. This ensures that the excitation beam emitted by the laser emitter can be absorbed by the high-dispersion lens 303, reducing light loss entering the lens and improving imaging quality.
[0089] In an embodiment of the present invention, the center of the filter 302 coincides with the optical axis of the high-dispersion lens 303; the optical signal contains at least three different colors of visible light, and each visible light corresponds to a focused spot; after the optical signal passes through the high-dispersion lens 303, a plurality of focused spots are generated on the focal plane; wherein the radius of the largest-sized spot in the focused spots is at least 1.414 times the radius of the smallest-sized spot. Preferably, max(A, B, C)>min(A, B, C)*sqrt(2), wherein sqrt(2) represents the square root of 2, and A, B, and C correspond to the spot radius of a color of visible light respectively, wherein the maximum spot radius is the minimum spot radius multiplied by the square root of 2. After the optical signal passes through a high-dispersion lens provided by an embodiment of the present invention, the focused spot sizes corresponding to the different colors of visible light are also enlarged or reduced to varying degrees. Among the formed focused spots, the radius of the largest-sized spot is at least 1.414 times the radius of the smallest-sized spot, and the overlapping range of the focused spots of different colors of visible light is reduced, further achieving the effect of separating different wavelengths. Preferably, in the embodiment of the present invention, the excitation light can be visible light or ultraviolet light.
[0090] In this embodiment of the present invention, the high-dispersion lens 303 further includes a photoelectric converter and a data converter. Specifically, the photoelectric converter is configured to generate a corresponding charge based on the received optical signal; the data converter is configured to read the accumulated charge from the photoelectric converter and convert it into a corresponding digital quantized value to achieve separation of the excitation light and the detection light. Preferably, the photoelectric converter is a photodiode, and the data converter can be a magic converter.
[0091] The detection module is configured to identify the detection light after passing through the high-dispersion lens to obtain a biometric identification result of the object to be detected. Specifically, the detection module selects a corresponding detection method based on the type of the object to be detected to identify the detection light to obtain the biometric identification result of the object to be detected. Preferably, the concentration of microorganisms in the object to be detected can be obtained by analyzing the electrical signal intensity of the detection light.
[0092] The high-dispersion lens in the embodiment of the present invention is implemented as a high-dispersion lens assembly as described in Example 1. Those skilled in the art will readily appreciate that, for ease and brevity of description, the specific operating process of the high-dispersion lens described above can refer to the corresponding operating process of the high-dispersion lens assembly in Example 1, and will not be further elaborated here.
[0093] An embodiment of the present invention provides a biological detection system in which a high-dispersion lens employs the high-dispersion lens assembly described above, exhibiting excellent high-dispersion capabilities and capable of focusing light of different wavelengths at different locations on the same focal plane. This application provides a biological detection system employing this high-dispersion lens, which is suitable for applications such as biofluorescence detection and wavelength separation.
[0094] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention. These improvements and substitutions should also be regarded as the scope of protection of the present invention.
Claims
1. A high dispersion lens assembly, characterized in that: include: The high dispersion lens assembly is composed of a receiving element, a relay element and a focusing element in the order of the incident direction of the light; The receiving element is used to receive the excitation light beam and disperse the excitation light into multiple beams of dispersed light according to wavelength; The relay element is used to focus each of the dispersed lights having the same wavelength to generate a relay dispersed light group; wherein the relay element comprises a lens group composed of a plurality of positive lenses and negative lenses, wherein each of the positive lenses is arranged in descending order according to the Abbe coefficient; and at least one of the positive lenses in the relay element is made of polycarbonate or a modified alkane high-dispersion material; The focusing element is used to focus the focused light spot corresponding to each relay dispersed light group to different positions on the same focal plane; wherein, the focusing element includes at least two lenses with different refractive indices, and the lenses are arranged in order from small to large according to the refractive index.
2. The high dispersion lens assembly according to claim 1, wherein: The receiving element includes a first receiving lens and a second diverging lens, specifically including: The first receiving lens is a convex lens, and the second diverging lens is a plano-concave lens; The first receiving lens is used to convert the received excitation light beam into parallel light; The second diverging lens is used to disperse the parallel light into the dispersed light according to wavelength; wherein the concave surface of the plano-concave lens faces the side of the excitation light beam.
3. The high dispersion lens assembly according to claim 1, wherein: The relay element includes at least two types of lenses: a biconvex positive lens and a meniscus positive lens.
4. A high dispersion lens design method, characterized in that: The high-dispersion lens assembly according to any one of claims 1 to 3, specifically: receiving an excitation light beam and dispersing the excitation light into multiple dispersed light beams according to wavelength; focusing each of the dispersed lights having the same wavelength to generate a relay dispersed light group; The focused light spots corresponding to each relay dispersed light group are focused to different positions on the same focal plane.
5. A biological detection system, characterized in that: include: A laser emitter, an optical filter, a high-dispersion lens, and a detection module; wherein the high-dispersion lens comprises the high-dispersion lens assembly according to any one of claims 1 to 3, specifically: The laser emitter is used to emit stable excitation light to the object to be measured so that the object to be measured generates detection light; The optical filter is used to filter the optical signal entering the high-dispersion lens; wherein the optical filter is arranged between the laser emitter and the high-dispersion lens, and the optical signal includes the excitation light and the detection light; The high dispersion lens is used to separate the excitation light and the detection light; The detection module is used to identify the detection light after passing through the high-dispersion lens to obtain a biometric recognition result of the object to be detected.
6. A biological detection system according to claim 5, characterized in that: The biological detection system further includes a reflector, specifically: The reflector is arranged between the laser emitter and the high-dispersion lens at a preset angle so that the excitation light emitted by the laser emitter is in a perpendicular relationship with the high-dispersion lens.
7. A biological detection system according to claim 5, characterized in that: The center of the optical filter coincides with the optical axis of the high dispersion lens; The optical signal includes at least three different colors of visible light, and each of the visible lights is focused on a corresponding light spot; After the optical signal passes through the high-dispersion lens, a plurality of focused light spots are generated on a focal plane; wherein the radius of the largest-sized light spot among the focused light spots is at least 1.414 times the radius of the smallest-sized light spot.
8. A biological detection system according to claim 5, characterized in that: The high dispersion lens further includes a photoelectric converter and a data converter, specifically: The photoelectric converter is used to generate corresponding charges according to the received light signal; The data converter is used to read the charge accumulated by the photoelectric converter and convert it into a corresponding digital quantization value, so as to achieve separation of the excitation light and the detection light.
9. A biological detection system according to claim 5, characterized in that: The detection module is used to identify the detection light after passing through the high-dispersion lens to obtain the biometric recognition result of the object to be detected, specifically: The detection module selects a corresponding detection method according to the category of the object to be detected to identify the detection light, so as to obtain a biometric recognition result of the object to be detected.
Citation Information
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