A multimodal elasticity detection device based on Brillouin-optical coherence-speckle

Abstract

The present invention discloses a multimodal elasticity detection device based on Brillouin-optical coherence-speckle, comprising a Brillouin-optical coherence elasticity imaging common-path scanning unit, a Brillouin scattering elasticity imaging system, an optical coherence elasticity imaging system, a speckle elasticity imaging system, and a timing controller. The Brillouin scattering elasticity imaging system and the optical coherence elasticity imaging system use the Brillouin-optical coherence elasticity imaging common-path scanning unit. The present invention utilizes the advantages of Brillouin scattering elasticity imaging in detecting bulk elastic modulus with high precision, optical coherence elasticity imaging in rapidly obtaining the elasticity distribution of the entire sample for three-dimensional elastic mapping, and speckle elasticity imaging in wide-field elasticity detection, to synchronously image the elasticity distribution of diseased tissue in situ, thereby providing a scientific basis and technical support for the early diagnosis of clinical diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of imaging devices, and in particular to a multimodal elasticity detection device based on Brillouin-optical coherence-speckle. Background Art

[0002] As an elasticity measurement device, the present invention mainly combines three technologies: Brillouin scattering elastography (BSE), optical coherence elastography (OCE) and speckle elastography (LSE) to detect the elastic distribution of biological tissues. The idea of ​​the invention is that for pathological tissues such as malignant tumors, brain injuries, skin cancer, etc., structural images cannot fully determine the development of the disease in the early stages of the disease. The biomechanical properties of tissues are of great significance for evaluating tissue function and status, and provide unique information for clinical disease prevention, diagnosis and monitoring. Due to changes in structure and interaction at the molecular, cellular and tissue levels, differences in biomechanical properties have been evaluated in traditional clinical practice. For example, palpation is a classic method for evaluating changes in biomechanical properties and has been widely used to detect breast cancer, evaluate abdominal organ function and diagnose skin diseases. However, palpation can only qualitatively evaluate the development of the disease based on tissue hardness, relying on personal experience and lacking clinical standards. Therefore, studying a new technology to obtain elastic information of biological tissues has important application value and significance for human health and disease diagnosis.

[0003] In elastography, Brillouin scattering is an inelastic scattering process whose spectral characteristics are closely related to the properties of the medium (such as density, viscosity, and elastic modulus). Therefore, Brillouin scattering elastography (BSE) can be used to measure the bulk elastic modulus of biological tissues by calculating the Brillouin frequency shift. Optical coherence elastography (OCE) is a novel elasticity measurement method based on optical coherence tomography. It quantifies the elastic moduli (such as shear modulus and Young's modulus) of biological tissues by detecting elastic wave propagation within biological tissues, enabling high-resolution and non-invasive assessment of the biomechanical properties of biological tissues. Speckle elastography (LSE) involves the backscattering of incident light by scattering particles on the tissue surface. Due to the different optical path lengths of the scattered light reaching the camera imaging plane, random interference occurs on the image plane, resulting in a spatially varying pattern of particles with varying brightness. During imaging, sample vibration is generated by an external excitation device, and the propagation of Rayleigh waves is tracked by a speckle contrast imaging system to calculate the elastic modulus and viscosity coefficient of the sample.

[0004] Therefore, the BSE-OCE-LSE multimodal elastic imaging system can synchronously measure the biomechanical properties of diseased tissues in situ, thus providing a scientific basis for the prevention and diagnosis of diseases. Summary of the Invention

[0005] The purpose of the present invention is to solve the technical problems existing in the prior art and to provide a multimodal imaging device for elasticity detection.

[0006] To achieve the above objectives, the present invention provides a technical solution: a multimodal elasticity detection device based on Brillouin-optical coherence-speckle, comprising a Brillouin-optical coherence elastic imaging common-path scanning unit, a Brillouin scattering elastic imaging system, an optical coherence elastic imaging system, a speckle elastic imaging system, and a timing controller, wherein the Brillouin scattering elastic imaging system and the optical coherence elastic imaging system share the Brillouin-optical coherence elastic imaging common-path scanning unit; the Brillouin-optical coherence elastic imaging common-path scanning unit comprises a dichroic mirror, a scanning galvanometer, a first-order optical shutter, and an objective lens;

[0007] The Brillouin scattering elastic imaging system includes a Brillouin signal excitation unit and a Brillouin signal acquisition unit, wherein the Brillouin signal excitation unit is used to excite the Brillouin scattering signal; the Brillouin signal acquisition unit is used to acquire the Brillouin spectrum signal to calculate the bulk elastic modulus;

[0008] The optical coherence elastic imaging system includes an optical coherence tomography unit and an acoustic radiation force excitation unit. The optical coherence tomography unit is used to collect interference signals generated inside the sample; the acoustic radiation force excitation unit is used to vibrate the sample to generate wave propagation.

[0009] The speckle elastic imaging system includes a speckle signal excitation unit and a speckle signal acquisition unit; the speckle signal excitation unit is used to excite the wide-field speckle signal generated on the sample surface; the speckle signal acquisition unit is used to collect the wide-field speckle signal generated on the sample surface to generate elasticity information;

[0010] The timing controller is mainly used for the step-by-step operation of a Brillouin scattering elastic imaging system, an optical coherence elastic imaging system and a speckle elastic imaging system.

[0011] Preferably, the Brillouin scattering signal excitation unit is composed of a narrow-linewidth continuous laser, a first optical fiber isolator, an optical fiber delay line, an optical fiber circulator, a first collimator and a beam splitter; the Brillouin signal acquisition unit is composed of a fifth collimator, a Brillouin spectrometer and a first detector; in the Brillouin elastic imaging system, the narrow-linewidth continuous laser emits a light beam, which is split by the beam splitter after passing through the first optical fiber isolator, the optical fiber delay line, port 1 of the optical fiber circulator, port 2 of the optical fiber circulator and the first collimator; the reflected light is focused by the objective lens onto the sample surface after passing through the dichroic mirror, the galvanometer group and the first optical shutter, and generates backward Brillouin scattering after interacting with the sample; the backward Brillouin scattered light of the sample returns along the original optical path, is output from port 3 of the optical fiber circulator, is collimated by the fifth collimator, enters the Brillouin spectrometer for frequency demodulation, and is received by the first detector.

[0012] Preferably, the Brillouin scattering elastic imaging system, the optical coherence elastic imaging system and the speckle elastic imaging system are integrated, and the light beams emitted by the laser are simultaneously made to reach the sample for detection through a timing controller and an optical fiber delay line, thereby realizing in-situ synchronous measurement of the sample elasticity with a wide field of view, high speed and high precision.

[0013] Preferably, the optical coherence tomography unit is composed of an ultra-wideband light-emitting diode, a second optical fiber isolator, a 2*2 optical fiber coupler, a second collimator, a reference arm, a grating spectrometer, and a linear array CCD21; the acoustic radiation force excitation unit is composed of a signal generator, a power amplifier and an ultrasonic transducer; in the optical coherence elasticity imaging system, the ultra-wideband light-emitting diode emits a light beam, which is collimated by the second optical fiber isolator, the 2*2 optical fiber coupler and the second collimator, and then reflected by the dichroic mirror. The reflected light passes through the galvanometer group and the first optical shutter, and is focused by the objective lens onto the sample to be measured. The backscattered light generated by the interaction with the sample returns along the original path, interferes with the light beam returned by the reference arm in the 2*2 optical fiber coupler, enters the grating spectrometer, and is collected and received by the linear array CCD; at the same time, the trigger signal synchronization signal generator generated by the optical coherence elasticity imaging system generates a sine wave signal, which is amplified by the power amplifier and then excited by the ultrasonic transducer, causing the sample to vibrate and be collected by the optical coherence tomography unit in the optical coherence elasticity imaging system.

[0014] Preferably, the ultrasonic transducer includes an ultrasonic generating unit and an impedance matching coupling agent, and the impedance matching coupling agent is mainly attached to the ultrasonic generating unit for matching a third medium to reduce the attenuation of the sound radiation power when propagating in the air.

[0015] Preferably, the speckle signal excitation unit comprises a narrow-linewidth continuous laser, a first optical fiber isolator, an optical fiber delay line, an optical fiber circulator, a first collimator, a beam splitter, a second optical shutter, a second plane reflector, and a beam expander; the speckle signal acquisition unit comprises an objective lens, a flip reflector, and a second detector. In the speckle detection system, the narrow-linewidth continuous laser emits a light beam, which passes through the first optical fiber isolator, the optical fiber delay line, port 1 of the optical fiber circulator, port 2 of the optical fiber circulator, and the first collimator, and is then split by the beam splitter. The light beam transmitted through the beam splitter passes through the second optical shutter, the second plane reflector, and the beam expander, and is incident on a sample to be measured. The light beam interacts with the sample to be measured to generate a scattered signal, which passes through the objective lens and is reflected by the flip reflector to the second detector for detection.

[0016] Preferably, the speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common-path scanning unit are alternately used through the first optical shutter, the second optical shutter, and the flip mirror, thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common-path scanning unit.

[0017] Preferably, the Brillouin-optical coherence elastic imaging common path scanning unit scans the sample on the XY plane through a galvanometer group to obtain the OCE three-dimensional elastic distribution, and at the same time, by adjusting the focal length of the objective lens, scans the sample at different depths to obtain a three-dimensional Brillouin elastic distribution image.

[0018] Beneficial effects of the present invention:

[0019] The present invention utilizes the advantages of Brillouin scattering elastography, which can detect bulk elastic modulus with high precision, optical coherence elastography, which can quickly obtain the elastic distribution of the entire sample for three-dimensional elastic mapping, and speckle elastography, which can detect elasticity in a wide field, to synchronously image the elastic distribution of diseased tissue in situ, thereby providing a scientific basis and technical support for the early diagnosis of clinical diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings described herein are used to provide further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0021] Figure 1 The invention relates to a multimodal elastic detection imaging device comprising Brillouin scattering imaging, optical coherence elastic imaging and speckle elastic imaging.

[0022] Figure annotation:

[0023] 1- Narrow-linewidth continuous laser 2- First fiber isolator 3- Fiber delay line 4- Fiber circulator 5- First collimator 6- Beam splitter 7- Ultra-wideband light-emitting diode 8- Second fiber isolator 9- 2*2 fiber coupler 10- Second collimator 11- Dichroic mirror 12- Galvanometer assembly 13- First optical shutter 14- Objective lens 15- Third collimator 16- Attenuator 17- First plane mirror 18- Fourth collimator 19- Grating spectrometer 20- Plano-convex lens 21- Linear array CCD 22- Fifth collimator 23- Brillouin spectrometer 24- First detector 25- Second optical shutter 26- Second plane mirror 27- Beam expander 28- Flip mirror 29- Second detector 30- Sample 31- Timing controller 32- Computer 33- Signal generator 34- Power amplifier 35- Ultrasonic transducer DETAILED DESCRIPTION

[0024] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it should not be understood as a limitation on the scope of protection of the present invention.

[0025] In the description of the present invention, "several" means one or more, "many" means more than two, "greater than," "less than," and "exceed" are understood to exclude the number itself, while "above," "below," and "within" are understood to include the number itself. The use of "first" and "second" in the description is solely for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.

[0026] Reference Figure 1 A preferred embodiment of the present invention is a multimodal elasticity detection device based on Brillouin-optical coherence-speckle, comprising a Brillouin-optical coherence elasticity imaging common-path scanning unit, a Brillouin scattering elasticity imaging system, an optical coherence elasticity imaging system, a speckle elasticity imaging system, and a timing controller 31. The Brillouin scattering elasticity imaging system and the optical coherence elasticity imaging system share the Brillouin-optical coherence elasticity imaging common-path scanning unit; the Brillouin-optical coherence elasticity imaging common-path scanning unit is composed of a dichroic mirror 11, a scanning galvanometer 12, a first optical shutter 13, and an objective lens 14, and is used to simultaneously acquire a Brillouin signal and an optical interference signal of a sample 30. Specifically, the Brillouin-optical coherence elasticity imaging common-path scanning unit realizes common optical path propagation and excitation through the dichroic mirror 11, so that the Brillouin scattering elasticity imaging system and the optical coherence elasticity imaging system realize in-situ synchronous detection;

[0027] The Brillouin scattering elastic imaging system includes a Brillouin signal excitation unit and a Brillouin signal acquisition unit, wherein the Brillouin signal excitation unit is used to excite the Brillouin scattering signal; the Brillouin signal acquisition unit is used to acquire the Brillouin spectrum signal to calculate the bulk elastic modulus;

[0028] The optical coherence elastic imaging system includes an optical coherence tomography unit and an acoustic radiation force excitation unit. The optical coherence tomography unit is used to collect interference signals generated inside the sample 30; the acoustic radiation force excitation unit is used to vibrate the sample 30 to generate wave propagation.

[0029] The speckle elastic imaging system includes a speckle signal excitation unit and a collection unit; the speckle signal excitation unit is used to excite the wide-field speckle signal generated on the surface of the sample 30; the speckle signal collection unit is used to collect the wide-field speckle signal generated on the surface of the sample 30 to generate elasticity information;

[0030] The timing controller 31 is mainly used for the step-by-step operation of the Brillouin scattering elastic imaging system, the optical coherence elastic imaging system and the speckle elastic imaging system.

[0031] As a preferred embodiment of the present invention, it may also have the following additional technical features:

[0032] In this embodiment, the Brillouin scattering signal excitation unit is composed of a narrow-linewidth continuous laser 1, a first optical fiber isolator 2, an optical fiber delay line 3, an optical fiber circulator 4, a first collimator 5 and a beam splitter 6; the Brillouin signal acquisition unit is composed of a fifth collimator 22, a Brillouin spectrometer 23 and a first detector 24; in the Brillouin elastic imaging system, the narrow-linewidth continuous laser 1 emits a light beam, which passes through the first optical fiber isolator 2, the optical fiber delay line 3, port 1 of the optical fiber circulator 4, port 2 of the optical fiber circulator 4 and the first collimator 5 and is then split by the beam splitter 6. The reflected light passes through the dichroic mirror 11, the galvanometer group 12 and the first optical shutter 13, and is focused by the objective lens 14 onto the surface of the sample 30. After interacting with the sample 30, backward Brillouin scattering is generated. The backward Brillouin scattered light of the sample 30 returns along the original optical path, is output from port 3 of the optical fiber circulator 4, is collimated by the fifth collimator 22, enters the Brillouin spectrometer 23 for frequency demodulation, and is received by the first detector 24.

[0033] In this embodiment, the light beams emitted by the Brillouin scattering elastic imaging system, the optical coherence elastic imaging system, and the speckle elastic imaging system simultaneously reach the sample 30 for detection through the timing controller 31 and the optical fiber delay line 3, thereby realizing in-situ synchronous measurement of the elasticity of the sample 30 with a wide field of view, high speed, and high precision.

[0034] In this embodiment, the optical coherence tomography unit is composed of an ultra-wideband light-emitting diode 7, a second optical fiber isolator 8, a 2*2 optical fiber coupler 9, a second collimator 10, a reference arm, a grating spectrometer 19, and a linear array CCD 21; the acoustic radiation force excitation unit is composed of a signal generator 33, a power amplifier 34 and an ultrasonic transducer 35; in the optical coherence elastic imaging system, the ultra-wideband light-emitting diode 7 emits a light beam, which is collimated by the second optical fiber isolator 8, the 2*2 optical fiber coupler 9 and the second collimator 10, and then reflected by the dichroic mirror 11. The reflected light is transmitted through the galvanometer group 11 and After the first optical shutter 13, the objective lens 14 focuses on the sample 30 to be measured, and the backscattered light generated by the interaction with the sample 30 returns along the original path, interferes with the light beam returned by the reference arm in the 2*2 fiber coupler 9, enters the grating spectrometer 19, and is collected and received by the linear array CCD21; at the same time, the trigger signal synchronization signal generator 33 generated by the optical coherence elasticity imaging system generates a sine wave signal, which is amplified by the power amplifier 34 and then excited by the ultrasonic transducer 34, causing the sample 30 to vibrate and be collected by the optical coherence tomography unit in the optical coherence elasticity imaging system.

[0035] Specifically, the reference arm includes a third collimator 15 , an attenuator 16 , and a first plane reflector 17 , and the grating spectrometer 19 includes a fourth collimator 18 , a grating, and a plano-convex lens 20 .

[0036] In this embodiment, the ultrasonic transducer 35 includes an ultrasonic generating unit and an impedance matching coupling agent. The impedance matching coupling agent is mainly attached to the ultrasonic generating unit to match the third medium to reduce the attenuation of the sound radiation power when propagating in the air.

[0037] In this embodiment, the speckle signal excitation unit comprises a narrow-linewidth continuous laser 1, a first fiber isolator 2, a fiber delay line 3, a fiber circulator 4, a first collimator 5, a beam splitter 6, a second optical shutter 25, a second plane mirror 26, and a beam expander 27. The speckle signal acquisition unit comprises an objective lens 14, a flip mirror 28, and a second detector 29. In the speckle detection system, the narrow-linewidth continuous laser 1 emits a light beam, which passes through the first fiber isolator 2, the fiber delay line 3, port 1 and port 2 of the fiber circulator 4, and the first collimator 5, and is then split by the beam splitter 6. The light beam transmitted through the beam splitter 6 passes through the second optical shutter 25, the second plane mirror 26, and the beam expander 27, and is incident on a sample 30 to be measured. The light beam interacts with the sample 30 to generate a scattered signal, which passes through the objective lens 14 and is reflected by the flip mirror 28 to the second detector 29 for detection.

[0038] Specifically, the speckle signal receiving unit reflects the speckle signal for signal acquisition when the speckle detection system detects the sample 30 by flipping the reflector 28. When the speckle detection system is not working, the reflector 28 is flipped again to realize the transmission of the detection light of the Brillouin-optical coherence elastic imaging common path scanning unit.

[0039] In this embodiment, the speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common path scanning unit are alternately used through the first optical shutter 13, the second optical shutter 25, and the flip mirror 28, thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common path scanning unit.

[0040] In this embodiment, the Brillouin-optical coherence elastic imaging common path scanning unit realizes scanning on the XY plane of the sample 30 through the galvanometer group 11 to obtain the three-dimensional elastic distribution of OCE. At the same time, by adjusting the focal length of the objective lens 14, it realizes scanning at different depths of the sample 30 to obtain a three-dimensional Brillouin elastic distribution image.

[0041] Specifically, when testing biological tissues, there are eight main steps:

[0042] The first step is to activate the optical coherence tomography system. An optical coherence elastography system without an acoustic radiation force excitation unit can be roughly considered an optical coherence tomography system for detecting tissue structure. The second optical shutter 25 is closed to block the light beam from entering the speckle signal excitation unit. The first optical shutter 13 is opened, and the flip mirror 28 is rotated away from the optical path, allowing the light beam after the galvanometer group 12 to pass through the first optical shutter 13 and enter the objective lens 14.

[0043] In the second step, the optical coherence tomography system performs tissue structure detection; specifically, in the optical coherence tomography unit of the optical coherence elasticity imaging system, the 850nm excitation light released by the ultra-wideband light emitting diode 7 passes through the second optical fiber isolator 8 and the 2*2 optical fiber coupler 9, and is collimated by the second collimator 10, and enters the Brillouin-optical coherence elasticity imaging common path scanning unit. The backscattered signal returning along the original path is reflected by the dichroic mirror 11, passes through the second collimator 10, enters the 2*2 optical fiber coupler 9, and is output by the fourth collimator 18. The output scattered signal is split by the grating spectrometer 19, and is focused by the plano-convex lens 20 to the linear array CCD 21 to collect the signal, and the collected signal is processed by the computer 32 to obtain the biological tissue structure information.

[0044] The third step is to switch the system and start the speckle detection system; open the second optical shutter 25 to allow the light beam to enter the speckle signal excitation unit, close the first optical shutter 13, and flip the reflector 28 to allow the light beam to enter the collection optical path of the speckle elastic imaging system.

[0045] In the fourth step, the acoustic radiation force excitation unit is turned on to generate a low-frequency ultrasonic signal. The acoustic radiation force system applies an external force to the sample 30, causing elastic waves to propagate in the sample 30. The specific details are as follows:

[0046] ① The timing controller 31 generates an external trigger signal which is connected to the signal generator 33 via a connecting line to generate a sine wave modulation signal, which is amplified by the power amplifier 34 and drives the ultrasonic transducer 35 to generate an excitation force.

[0047] ② The impedance matching coupling agent in the ultrasonic transducer 35 is mainly attached to the ultrasonic generating unit to match the third medium to reduce the attenuation of the sound radiation power when propagating in the air.

[0048] In the fifth step, the speckle elastic imaging system performs wide-field elastic imaging on the sample 30 to detect the propagation process of elastic waves in the tissue within the wide field of view. The specific details are as follows:

[0049] ① When the speckle elastic imaging system performs wide-field scanning, the second optical shutter 25 is opened to turn on the speckle signal excitation unit; the flip mirror 28 is rotated into the optical path to turn on the speckle signal receiving unit.

[0050] ② In the speckle signal excitation unit, a beam of light split by the beam splitter 6 passes through the second optical shutter 25, is reflected by the second plane reflector 26, and then enters the beam expander 27 for beam expansion. It hits the sample 30 in the form of a wide-field light spot and interacts with the sample 30.

[0051] ③ Speckle signal receiving unit: the speckle signal generated at the sample 30 passes through the objective lens 14, is reflected by the flip mirror 28 and enters the detector 29, collects the speckle signal, and uses the computer 32 to process the collected signal.

[0052] Step 6: Switch systems; turn off the speckle detection system, turn on the Brillouin scattering elastography system and the optical coherence elastography system; close the second optical shutter 25 to block the light beam from entering the speckle signal excitation unit, open the first optical shutter 13, and rotate the flip mirror 28 away from the light path, so that the light beam after the galvanometer group 12 can pass through the first optical shutter 13 and enter the objective lens 14.

[0053] In the seventh step, the acoustic radiation force excitation unit is turned on to generate a high-frequency ultrasonic signal. The acoustic radiation force excitation unit applies an external force to the sample 30 to generate elastic wave propagation in the sample 30 .

[0054] In the eighth step, when the Brillouin scattering elastography system and the optical coherence elastography system simultaneously detect the elastic modulus of sample 30, the Brillouin scattering elastography system obtains the bulk elastic modulus of the tissue with high precision, and the optical coherence elastography system rapidly obtains the tissue elastic distribution image through its high speed and high sensitivity. The Brillouin scattering elastography system and the optical coherence elastography system share the Brillouin-optical coherence elastography scanning unit, which can realize in-situ synchronous detection of the Brillouin scattering elastography system and the optical coherence elastography system. The specific details are as follows:

[0055] ① The shared Brillouin-optical coherence elastic imaging scanning unit mainly includes a dichroic mirror 11, a galvanometer mirror group 12, a first optical shutter 13 and an objective lens 14. The dichroic mirror 11 transmits the excitation light in the Brillouin scattering elastic imaging system into the Brillouin-optical coherence elastic imaging common-path scanning unit, and reflects the excitation light in the optical coherence elastic imaging system into the Brillouin-optical coherence elastic imaging system common-path scanning unit. The light beam entering the Brillouin-optical coherence elastic imaging system common-path scanning unit is finally focused on the sample 30, interacting with the sample 30 to generate a scattering signal;

[0056] ② In the Brillouin scattering signal excitation unit, a narrow-linewidth continuous laser 1 releases 780nm excitation light, which enters port 1 of a circulator 4 after passing through a first fiber isolator 2 and a fiber delay line 3. The spatial light output from port 2 of the circulator 4 is collimated by a first collimator 5 and split by a beam splitter 6. Part of the light enters the common-path scanning unit of the optical coherence elastic imaging system.

[0057] ③ In the optical coherence tomography unit of the optical coherence elastic imaging system, the 850nm excitation light emitted by the ultra-wideband light-emitting diode 7 passes through the second optical fiber isolator 8 and the 2*2 optical fiber coupler 9, is collimated by the second collimator 10, and enters the Brillouin-optical coherence elastic imaging system common path scanning unit;

[0058] ④ Adjust the timing controller 31 and the optical fiber delay line 3 to control the timing of the narrow linewidth continuous laser 1 and the ultra-wideband light emitting diode 7 to ensure that the light beams output by the narrow linewidth continuous laser 1 and the ultra-wideband light emitting diode 7 reach the dichroic mirror 11 at the same time;

[0059] ⑤ The signal light generated at the sample 30 returns along the original optical path from the Brillouin-optical coherence elastic imaging common path scanning unit and is collected by the signal receiving unit. The Brillouin scattering elastic imaging system and the optical coherence elastic imaging system have independent signal receiving units. In the Brillouin scattering signal receiving unit, the backscattered Brillouin scattering signal generated at the sample 30 returns along the original optical path, passes through the optical fiber circulator 4, is collimated by the fifth collimator 22, enters the Brillouin spectrometer 23, and is spectrally detected by the first detector 24. The collected spectral signal is processed by the computer 32;

[0060] ⑥ In the optical coherence elastic imaging system acquisition unit, the backscattered signal returning along the original path of the Brillouin-optical coherence elastic imaging common path scanning unit is reflected by the dichroic mirror 11, passes through the second collimator 10, enters the 2*2 fiber coupler 9, and is output by the fourth collimator 18. The output scattered signal is split by the grating spectrometer 19, and is focused by the plano-convex lens 20 to the linear array CCD 21 for signal acquisition, and the acquired signal is processed using the computer 32.

[0061] Under the premise that no conflict occurs, those skilled in the art may freely combine and superimpose the above-mentioned additional technical features.

[0062] The above descriptions are only preferred embodiments of the present invention. Any technical solution that achieves the purpose of the present invention by substantially the same means shall fall within the scope of protection of the present invention.

Claims

1. A multimodal elasticity detection device based on Brillouin-optical coherence-speckle, characterized by: The system comprises a Brillouin-optical coherence elastic imaging common path scanning unit, a Brillouin scattering elastic imaging system, an optical coherence elastic imaging system, a speckle elastic imaging system, a timing controller, and an optical fiber delay line. The Brillouin scattering elastic imaging system and the optical coherence elastic imaging system share the Brillouin-optical coherence elastic imaging common path scanning unit. The Brillouin-optical coherence elastic imaging common path scanning unit comprises a dichroic mirror, a scanning galvanometer, a first optical shutter, and an objective lens. The Brillouin scattering elastic imaging system includes a Brillouin signal excitation unit and a Brillouin signal acquisition unit, wherein the Brillouin signal excitation unit is used to excite the Brillouin scattering signal; The Brillouin signal acquisition unit is used to acquire Brillouin spectrum signals to calculate the bulk elastic modulus; The optical coherence elastic imaging system includes an optical coherence tomography unit and an acoustic radiation force excitation unit. The optical coherence tomography unit is used to collect interference signals generated inside the sample; the acoustic radiation force excitation unit is used to vibrate the sample to generate wave propagation. The speckle elastic imaging system includes a speckle signal excitation unit and a speckle signal acquisition unit; the speckle signal excitation unit is used to excite the wide-field speckle signal generated on the sample surface; the speckle signal acquisition unit is used to collect the wide-field speckle signal generated on the sample surface to generate elasticity information; The timing controller and optical fiber delay line are mainly used for in-situ synchronous measurement of Brillouin scattering elastic imaging system, optical coherence elastic imaging system and speckle elastic imaging; The Brillouin scattering signal excitation unit is composed of a narrow-linewidth continuous laser, a first optical fiber isolator, an optical fiber delay line, an optical fiber circulator, a first collimator and a beam splitter; the Brillouin signal acquisition unit is composed of a fifth collimator, a Brillouin spectrometer and a first detector; in the Brillouin scattering elastic imaging system, a narrow-linewidth continuous laser emits a light beam, which is split by the beam splitter after passing through the first optical fiber isolator, the optical fiber delay line, port 1 of the optical fiber circulator, port 2 of the optical fiber circulator and the first collimator; the reflected light is focused by the objective lens onto the sample surface after passing through the dichroic mirror, the galvanometer group and the first optical shutter, and generates back Brillouin scattering after interacting with the sample; the back Brillouin scattered light of the sample returns along the original optical path, is output from port 3 of the optical fiber circulator, is collimated by the fifth collimator, enters the Brillouin spectrometer for frequency discrimination, and is received by the first detector; The speckle signal excitation unit comprises a narrow-linewidth continuous laser, a first fiber isolator, a fiber delay line, a fiber circulator, a first collimator, a beam splitter, a second optical shutter, a second plane reflector, and a beam expander. The speckle signal acquisition unit comprises an objective lens, a flip reflector, and a second detector. In the speckle elasticity detection system, a narrow-linewidth continuous laser emits a light beam, which passes through the first fiber isolator, the fiber delay line, port 1 and port 2 of the fiber circulator, and the first collimator, and is then split by the beam splitter. The light beam transmitted through the beam splitter passes through the second optical shutter, the second plane reflector, and the beam expander, and is incident on a sample to be tested. The light beam interacts with the sample to be tested to generate a scattered signal, which passes through the objective lens and is reflected by the flip reflector to the second detector for detection.

2. The multimodal elasticity detection device based on Brillouin-optical coherence-speckle according to claim 1, characterized in that: The optical coherence tomography unit consists of an ultra-wideband light-emitting diode, a second fiber isolator, a 2*2 fiber coupler, a second collimator, a reference arm, a grating spectrometer, and a linear array CCD; the acoustic radiation force excitation unit consists of a signal generator, a power amplifier, and an ultrasonic transducer; in the optical coherence elasticity imaging system, the ultra-wideband light-emitting diode emits a light beam, which is collimated by the second fiber isolator, the 2*2 fiber coupler, and the second collimator, and then reflected by the dichroic mirror. The reflected light passes through the galvanometer group and the first optical shutter, and is focused by the objective lens onto the sample to be measured. The backscattered light generated by the interaction with the sample returns along the original path, interferes with the light beam returned by the reference arm in the 2*2 fiber coupler, enters the grating spectrometer, and is collected and received by the linear array CCD; at the same time, the trigger signal generated by the optical coherence elasticity imaging system synchronizes the signal generator to generate a sine wave signal, which is amplified by the power amplifier and then excited by the ultrasonic transducer to cause the sample to vibrate and be collected by the optical coherence tomography unit in the optical coherence elasticity imaging system.

3. The multimodal elasticity detection device based on Brillouin-optical coherence-speckle according to claim 2, characterized in that: The Brillouin scattering elastic imaging system, optical coherence elastic imaging system and speckle elastic imaging system are integrated, and the light beams emitted by the narrow-linewidth continuous laser and the ultra-wideband light-emitting diode are controlled by a timing controller and an optical fiber delay line to reach the sample at the same time for detection.

4. The multimodal elasticity detection device based on Brillouin-optical coherence-speckle according to claim 3, characterized in that: The ultrasonic transducer includes an ultrasonic generating unit and an impedance matching coupling agent. The impedance matching coupling agent is mainly attached to the ultrasonic generating unit to match the third medium to reduce the attenuation of the sound radiation power when propagating in the air.

5. The multimodal elasticity detection device based on Brillouin-optical coherence-speckle according to claim 1, characterized in that: The speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common path scanning unit are alternately used through the first optical shutter, the second optical shutter and the flip mirror, thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-optical coherence elastic imaging common path scanning unit.

6. The multimodal elasticity detection device based on Brillouin-optical coherence-speckle according to claim 1, characterized in that: The Brillouin-Optical Coherence Elasticity Imaging common path scanning unit scans the sample on the XY plane through a galvanometer group to obtain the three-dimensional elastic distribution of OCE. At the same time, by adjusting the focal length of the objective lens, it scans different depths of the sample to obtain a three-dimensional Brillouin elastic distribution image.

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