A method and device for enriching and culturing anaerobic ammonia-oxidizing bacteria

Abstract

The present invention relates to an enrichment and cultivation method and device for anaerobic ammonia oxidizing bacteria, which is to inoculate activated sludge containing anaerobic ammonia oxidizing bacteria into an anaerobic membrane bioreactor, start water inlet, carry out enrichment and cultivation, collect gas generated during the cultivation process, mix it with hydrogen and then pass it into the reactor to carry out enrichment and cultivation of anaerobic ammonia oxidizing bacteria. The present invention realizes rapid enrichment and cultivation of anaerobic ammonia oxidizing bacteria, effectively slows down membrane pollution and prolongs the membrane service life.

Description

Technical Field

[0001] The invention belongs to the field of environmental microorganisms, and specifically relates to an enrichment and cultivation method and device for anaerobic ammonia-oxidizing bacteria. Background Art

[0002] Anaerobic ammonium oxidation technology has the advantages of high efficiency, energy saving, and environmental protection. Its discovery and proposal have made a big step forward in the development of wastewater biological denitrification technology towards low carbon and low consumption. Anaerobic ammonium oxidation technology has been quite mature in laboratory research. Currently, researchers at home and abroad have been committed to promoting the industrial application of anaerobic ammonium oxidation technology in the field of wastewater denitrification. Anaerobic ammonium oxidation technology is inseparable from anaerobic ammonium oxidizing bacteria, which are chemoautotrophic bacteria with slow reproduction, long doubling time, and are easily affected by environmental conditions. At present, the lack of anaerobic ammonium oxidizing bacteria has become the main bottleneck restricting the large-scale promotion of this technology. Therefore, achieving efficient enrichment and cultivation of anaerobic ammonium oxidizing bacteria has become a research hotspot.

[0003] There are two existing anaerobic ammonium oxidizing bacteria cultivation methods: batch operation and continuous operation. In the batch operation method, the sedimentation and drainage after the cultivation is completed can well intercept the anaerobic ammonium oxidizing bacteria and reduce the loss. However, during the cultivation process, continuous feeding is required as the reaction proceeds. In order to avoid the high nitrite nitrogen concentration after each feeding, which inhibits the activity of anaerobic ammonium oxidizing bacteria, Chen Yong et al. (Effect of nitrite nitrogen concentration on anaerobic ammonium oxidation reaction, China Water Supply and Drainage, 2006 (17): 74-76) can only use low-concentration feeding in their research. The low substrate concentration will affect the anaerobic ammonium oxidation reaction rate, thereby reducing the enrichment rate. The continuous operation method can well solve the problem of high nitrite nitrogen concentration inhibiting bacterial activity after feeding during batch operation cultivation. However, although a sedimentation tank is set up for continuous inlet and outlet water, there is still the problem of anaerobic ammonium oxidizing bacteria loss.

[0004] CN103087910A discloses a device and method for rapid enrichment, proliferation and purification and cultivation of anaerobic ammonia oxidizing bacteria, which is to first set a device for rapid enrichment, proliferation and purification and cultivation of anaerobic ammonia oxidizing bacteria; then place a certain amount of aerobic activated sludge after elutriation and filtration in a tank-type bioreactor, and mix it with a nutrient solution; gradually introduce a nutrient solution heated by a water inlet preheating system into the reaction tank; intercept anaerobic ammonia oxidizing bacteria through a cloth-like suspended fiber-type filler; after 60-90 days of cultivation, the cloth-like suspended fiber-type filler covered with brown-red anaerobic ammonia oxidizing bacteria is taken out, eluted, and naturally precipitated to obtain a large number and a high purity of anaerobic ammonia oxidizing denitrification sludge. The device and method can achieve the goal of rapid enrichment, proliferation and purification of anaerobic oxidizing bacteria, but the technology adopts a biofilm culture method, and a filler with a specific structure is required. In addition, in anaerobic systems, stirring is often used to achieve the problem of reducing membrane pollution, but stirring is not effective in reducing membrane pollution, and membrane pollution is more serious, requiring frequent disassembly and replacement of membrane components. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method and device for enriching and culturing anaerobic ammonia oxidizing bacteria. The present invention realizes rapid enrichment and cultivation of anaerobic ammonia oxidizing bacteria, effectively reduces membrane pollution, and prolongs the membrane service life.

[0006] The enrichment and cultivation method of anaerobic ammonia-oxidizing bacteria provided by the present invention comprises the following contents:

[0007] The activated sludge containing anaerobic ammonia-oxidizing bacteria is inoculated into the anaerobic membrane bioreactor, and water is started to be enriched and cultured. The gas generated during the culture process is collected, mixed with hydrogen, and then introduced into the reactor for enrichment culture of anaerobic ammonia-oxidizing bacteria.

[0008] In the present invention, the activated sludge containing anaerobic ammonia oxidizing bacteria is taken from activated sludge or residual activated sludge from industrial and urban sewage treatment plants, and contains denitrifying bacteria, decarbonizing bacteria, dephosphorizing bacteria and other bacteria, wherein the denitrifying bacteria include anaerobic ammonia oxidizing bacteria, aerobic ammonia oxidizing bacteria and denitrifying bacteria, etc. The activated sludge is inoculated according to the sludge concentration after inoculation of 4000-6000 mg / L.

[0009] In the present invention, the anaerobic membrane bioreactor may be a MBR membrane bioreactor well known in the art, and the membrane component may be a flat membrane, a hollow fiber membrane, or the like.

[0010] In the present invention, the influent contains ammonia nitrogen, nitrite nitrogen and inorganic carbon, wherein the total nitrogen concentration is controlled at 200-1500 mg / L, and the ratio of ammonia nitrogen to nitrite nitrogen is regulated according to 1:1.2-1.3. The substance providing ammonia nitrogen can be an ammonium salt, such as at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate, ammonium carbonate, etc., preferably at least one of ammonium sulfate, ammonium chloride, etc. The substance providing nitrite nitrogen can be a nitrite, such as at least one of sodium nitrite, potassium nitrite, etc. The substance providing inorganic carbon can be one or more of KHCO3, NaHCO3, etc., and the concentration of inorganic carbon is controlled at 100-150 mg / L.

[0011] In the present invention, a nutrient solution is added into the influent water, and the formula is: 0.02-0.025 g / LKH2PO4, 0.2-0.3 g / LCaCl2·2H2O, 0.1-0.2 g / LMgSO4·7H2O, 0.005-0.00625 g / LFeSO4, and 0.005-0.00625 g / LEDTA.

[0012] In the present invention, the enrichment culture conditions are: dissolved oxygen concentration is lower than 0.1 mg / L, temperature is 20-35° C., and pH is 7.0-8.0.

[0013] In the present invention, a gas collection system is provided on the top of the anaerobic membrane bioreactor to collect the gas generated during the enrichment culture process. The gas collection system mainly includes a gas storage facility with a gas collection function, such as a gas cabinet, a buffer tank, a gas collection bag, etc. The gas collection system may further include a drying device, and the collected gas enters the gas storage facility after drying, and then is mixed with hydrogen and transported to the anaerobic membrane bioreactor.

[0014] In the present invention, the collected gas is mixed with a certain amount of hydrogen before entering the anaerobic membrane bioreactor, and the volume proportion of hydrogen is 90%-95%.

[0015] In the present invention, the amount of gas introduced during the entire culture process is controlled by aeration intensity at 5-15m 3 / (m 2 h), preferably 8-12 m 3 / (m 2 ·h), where aeration intensity refers to the aeration volume per unit membrane area per unit time.

[0016] In the present invention, during the culture process, the inlet substrate concentration is increased in a gradient manner according to the effluent nitrite nitrogen concentration, that is, when the system effluent nitrite nitrogen concentration is lower than 20 mg / L, the inlet total nitrogen concentration is increased by 50-100 mg / L, and the enrichment culture is completed when the proportion of anaerobic ammonia oxidizing bacteria in the system increases by more than 1 times.

[0017] In the present invention, the culture process is preferably divided into two stages. In the first stage, an activator is added, and the activator is at least one of hydroxylamine, hydrazine, homoserine lactone, etc., and the addition concentration is 1-80 mg / L. When the total nitrogen volume load of the system reaches 1.0 kgN / m 3 / d or more, the first stage of cultivation is completed, the addition of activator is stopped, and the second stage of cultivation begins. When the proportion of anaerobic ammonia-oxidizing bacteria in the system increases by more than 1 times, the enrichment cultivation is completed.

[0018] In the present invention, during the entire culture process, the pressure of the anaerobic membrane bioreactor is regulated to be lower than 0.16 MPa.

[0019] The present invention also provides a device for the above-mentioned anaerobic ammonia-oxidizing bacteria enrichment and cultivation method, which mainly includes a feeding system, an anaerobic membrane bioreactor, a gas collection system, and a pressure control system; wherein the feeding system is used to adjust the water ratio and transport the water to the anaerobic membrane bioreactor; the anaerobic membrane bioreactor is used for the enrichment and cultivation of anaerobic ammonia-oxidizing bacteria; the gas collection system is used to collect the gas generated during the cultivation process and mix it with hydrogen and then transport it to the anaerobic membrane bioreactor; the pressure control system is used to regulate the pressure of the membrane bioreactor.

[0020] In the present invention, the gas collection system mainly includes a gas storage facility with a gas collection function, such as any one of a gas cabinet, a buffer tank, a gas collection bag, etc. The gas collection system may further include a drying device, and the collected gas enters the gas storage facility after drying, and then is mixed with hydrogen and transported to the anaerobic membrane bioreactor. The pressure control system mainly includes a pressure gauge and a safety pressure limiting valve.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] (1) According to the growth characteristics and product characteristics of anaerobic ammonium oxidizing bacteria, the present invention collects the gas generated during the growth of anaerobic ammonium oxidizing bacteria, mixes it with hydrogen, and then quantitatively introduces it into the anaerobic membrane bioreactor. On the one hand, the competitive advantage of anaerobic ammonium oxidizing bacteria is improved, the rapid enrichment culture of anaerobic ammonium oxidizing bacteria is achieved, and the enrichment time of anaerobic ammonium oxidizing bacteria is shortened; at the same time, the problem of membrane pollution and blockage is alleviated, and the membrane service life is extended.

[0023] (2) The present invention adopts a staged culture method according to the growth characteristics of anaerobic ammonium oxidizing bacteria. In the first stage, a mixed gas is introduced and combined with the synergistic effect of adding an activator, which effectively improves the selective and rapid growth of anaerobic ammonium oxidizing bacteria, and in particular, can further avoid membrane pollution.

[0024] (3) The anaerobic ammonia-oxidizing bacteria cultured by the method of the present invention have high activity and strong tolerance and are suitable for large-scale industrial applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of a process of the anaerobic ammonia oxidizing bacteria enrichment and cultivation device of the present invention;

[0026] Among them: 1-feed tank, 2-feed pump, 3-anaerobic membrane bioreactor, 4-membrane assembly, 5-aeration pipe, 6-pressure gauge, 7-water outlet pump, 8-air outlet, 9-drying tank, 10-air collecting bag, 11-safety pressure limiting valve, 12-diaphragm pump, 13-sludge outlet. DETAILED DESCRIPTION

[0027] The method and effects of the present invention are further described in detail below through examples. The examples are implemented based on the technical solution of the present invention, and provide detailed implementation methods and specific operation processes, but the protection scope of the present invention is not limited to the following examples.

[0028] The experimental methods in the following examples, unless otherwise specified, are all conventional methods in the art. The experimental materials used in the following examples, unless otherwise specified, can all be purchased from biochemical reagent stores.

[0029] In the embodiments of the present invention, the ammonia nitrogen concentration is determined by GB7478-87 "Water quality-Determination of ammonium-Distillation and titration method"; the nitrite nitrogen concentration is determined by GB7493-87 "Water quality-Determination of nitrite nitrogen-Spectrophotometry"; the total nitrogen concentration is determined by GB11894-89 "Water quality-Determination of total nitrogen-Alkaline potassium persulfate digestion ultraviolet spectrophotometry"; and the bacterial flora analysis is determined by metagenomic microbial classification sequencing.

[0030] The enrichment culture device used in the embodiment of the present invention is as shown in the attached Figure 1 As shown, the materials required for the growth of anaerobic ammonia-oxidizing bacteria are transported from the feed tank 1 to the anaerobic membrane bioreactor 3 through the feed pump 2. The water in the reactor is discharged through the membrane assembly 4 and the pressure gauge 6 under the action of the outlet pump 7. The gas generated during the culture process passes through the gas outlet 8 and enters the gas collecting bag 10 after passing through or not passing through the drying tank 9. The diaphragm pump 12 passes the collected gas into the anaerobic membrane bioreactor 3 through the aeration pipe 5. As the gas production increases, when the pressure in the anaerobic membrane bioreactor 3 reaches 0.16MPa, the safety pressure limiting valve 11 is opened to exhaust and release the pressure.

[0031] The activated sludge containing anaerobic ammonia-oxidizing bacteria used in the example was taken from a high-ammonia nitrogen water treatment system. The metagenomic microbial classification and sequencing showed that the proportion of anaerobic ammonia-oxidizing bacteria in the sludge was about 9.1%.

[0032] Example 1

[0033] The effective volume of the anaerobic membrane bioreactor is 5L, the membrane component is a flat membrane, and activated sludge containing anaerobic ammonia-oxidizing bacteria is inoculated. The MLSS after inoculation is 4000mg / L.

[0034] Prepare the influent water, the concentration of ammonia nitrogen is 100mg / L, the concentration of nitrite nitrogen is 130mg / L, and the concentration of inorganic carbon is 100mg / L. The substance that provides ammonia nitrogen is ammonium sulfate, the substance that provides nitrite nitrogen is sodium nitrite, and the substance that provides inorganic carbon is NaHCO3. Add nutrient solution to the influent water, the formula is: 0.025g / LKH2PO4, 0.3g / LCaCl2·2H2O, 0.2g / LMgSO4·7H2O, 0.00625g / LFeSO4, 0.00625g / LEDTA.

[0035] Start water inflow and carry out enrichment culture under the following conditions: dissolved oxygen concentration of 0.08 mg / L, temperature of 35°C, pH of 7.3, continuous water inflow and outflow, and hydraulic retention time controlled at 16 h.

[0036] The gases produced during the culture process were collected and mixed with hydrogen at a volume ratio of 95%, and then introduced into the anaerobic membrane bioreactor at a gas volume of 8m 3 / (m 2h). During the entire culture process, the pressure of the anaerobic membrane bioreactor was regulated to be lower than 0.16 MPa.

[0037] Three days after the device was started, the nitrite nitrogen concentration in the effluent was monitored to be less than 20 mg / L. The total nitrogen in the inlet was increased by a gradient method, with ammonia nitrogen: nitrite nitrogen = 1:1.2, and the total nitrogen concentration in the inlet was increased by 100 mg / L, and the culture continued. As the sludge activity and concentration gradually increased, the treatment capacity also continued to increase. On the 28th day, the total nitrogen concentration in the inlet was 1120 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.7% through metagenomic microbial classification sequencing, indicating that the enrichment culture was completed. During the entire culture process, the pressure reached 0.040 MPa on the 21st day, and the membrane components were replaced and cleaned.

[0038] Example 2

[0039] The effective volume of the anaerobic membrane bioreactor is 5L, the membrane component is a flat membrane, and activated sludge containing anaerobic ammonia-oxidizing bacteria is inoculated. The MLSS after inoculation is 6000mg / L.

[0040] Prepare the influent water, the concentration of ammonia nitrogen is 100mg / L, the concentration of nitrite nitrogen is 130mg / L, and the concentration of inorganic carbon is 150mg / L. The substance that provides ammonia nitrogen is ammonium chloride, the substance that provides nitrite nitrogen is potassium nitrite, and the substance that provides inorganic carbon is KHCO3. Add nutrient solution to the influent water, the formula is: 0.025g / LKH2PO4, 0.3g / LCaCl2·2H2O, 0.2g / LMgSO4·7H2O, 0.00625g / LFeSO4, 0.00625g / LEDTA.

[0041] Start water inflow and carry out enrichment culture under the following conditions: dissolved oxygen concentration of 0.08 mg / L, temperature of 35°C, pH of 7.3, continuous water inflow and outflow, and hydraulic retention time controlled at 16 h.

[0042] The gases produced during the culture process were collected and mixed with hydrogen at a volume ratio of 90%, and then introduced into the anaerobic membrane bioreactor with a ventilation volume of 12m 3 / (m 2 h). During the entire culture process, the pressure of the anaerobic membrane bioreactor was regulated to be lower than 0.16 MPa.

[0043] Two days after the device was started, the nitrite nitrogen concentration in the effluent was monitored to be less than 20 mg / L. The total nitrogen concentration in the inlet was increased by 100 mg / L in a gradient manner according to the ratio of ammonia nitrogen to nitrite nitrogen = 1:1.2, and the cultivation continued. As the activity and concentration of the sludge gradually increased, the treatment capacity also continued to increase. On the 26th day, the total nitrogen concentration in the inlet was 1180 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 19.3% through metagenomic microbial classification sequencing, indicating that the enrichment culture was completed. During the entire cultivation process, the pressure reached 0.042 MPa on the 24th day, and the membrane components were replaced and cleaned.

[0044] Example 3

[0045] The effective volume of the anaerobic membrane bioreactor is 5L, the membrane component is a flat membrane, and activated sludge containing anaerobic ammonia-oxidizing bacteria is inoculated. The MLSS after inoculation is 4000mg / L.

[0046] Prepare the influent water, the concentration of ammonia nitrogen is 100mg / L, the concentration of nitrite nitrogen is 130mg / L, and the concentration of inorganic carbon is 130mg / L. The substance that provides ammonia nitrogen is ammonium carbonate, the substance that provides nitrite nitrogen is sodium nitrite, and the substance that provides inorganic carbon is KHCO3. Add nutrient solution to the influent water, the formula is: 0.025g / LKH2PO4, 0.3g / LCaCl2·2H2O, 0.2g / LMgSO4·7H2O, 0.00625g / LFeSO4, 0.00625g / LEDTA.

[0047] Start water inflow and carry out enrichment culture under the following conditions: dissolved oxygen concentration of 0.08 mg / L, temperature of 35°C, pH of 7.3, continuous water inflow and outflow, and hydraulic retention time controlled at 16 h.

[0048] The gases produced during the culture process were collected and mixed with hydrogen at a volume ratio of 90%, and then introduced into the anaerobic membrane bioreactor with a ventilation volume of 12m 3 / (m 2 h). During the entire culture process, the pressure of the anaerobic membrane bioreactor was regulated to be lower than 0.16 MPa.

[0049] Three days after the device was started, the nitrite nitrogen concentration in the effluent was monitored to be less than 20 mg / L, and the ammonia nitrogen concentration in the inlet was increased by a gradient method, according to ammonia nitrogen: nitrite nitrogen = 1:1.2, and the total nitrogen concentration in the inlet was increased by 100 mg / L, and the culture continued. As the sludge activity and concentration gradually increased, the treatment capacity also continued to increase. On the 28th day, the total nitrogen concentration in the inlet was 1100 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.2% through metagenomic microbial classification sequencing, indicating that the enrichment culture was completed. During the entire culture process, the pressure reached 0.041 MPa on the 25th day, and the membrane components were replaced and cleaned.

[0050] Example 4

[0051] Same as Example 1, except that the enrichment culture was divided into two stages. In the first stage, hydroxylamine was added at a concentration of 45 mg / L. On the 8th day, the total nitrogen volume load of the system reached 1.1 kgN / m 3 / d, stop adding and proceed to the second stage of cultivation.

[0052] On the 17th day, the total nitrogen concentration of the influent was 1240 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 19.9% ​​through metagenomic microbial classification sequencing, indicating that the enrichment culture was completed. During the entire culture process, the membrane pressure of the membrane module reached a maximum of 0.033 MPa, and no cleaning was required.

[0053] Example 5

[0054] Same as Example 1, except that the enrichment culture was divided into two stages. In the first stage, hydrazine was added at a concentration of 5 mg / L. On the 9th day, the total nitrogen volume load of the system reached 1.01 kgN / m 3 / d, stop adding and proceed to the second stage of cultivation.

[0055] On the 18th day, the total nitrogen concentration of the influent was 1260 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.9% through metagenomic microbial classification sequencing, indicating that enrichment culture was completed. During the entire culture process, the membrane pressure of the membrane module reached a maximum of 0.031 MPa, and no cleaning was required.

[0056] Example 6

[0057] Same as Example 1, except that the enrichment culture was divided into two stages. In the first stage, homoserine lactone was added at a concentration of 30 mg / L. On the sixth day, the total nitrogen volume load of the system reached 1.03 kgN / m 3 / d, stop adding and proceed to the second stage of cultivation.

[0058] On the 16th day, the total nitrogen concentration of the influent was 1260 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 19.1% through metagenomic microbial classification sequencing, indicating that the enrichment culture was completed. During the entire culture process, the membrane pressure of the membrane module reached a maximum of 0.035 MPa, and no cleaning was required.

[0059] Example 7

[0060] Same as Example 1, except that the enrichment culture was divided into two stages. In the first stage, 18 mg / L hydroxylamine, 2 mg / L hydrazine, and 12 mg / L homoserine lactone were added. On the seventh day, the total nitrogen volume load of the system reached 1.02 kgN / m 3 / d, stop adding and proceed to the second stage of cultivation.

[0061] On the 14th day, the total nitrogen concentration of the influent was 1260 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.4% through metagenomic microbial classification sequencing, indicating that enrichment culture was completed. During the entire culture process, the membrane pressure of the membrane module reached a maximum of 0.031 MPa, and no cleaning was required.

[0062] Comparative Example 1

[0063] The same as Example 1, except that the volume proportion of hydrogen in the gas collecting bag is 80%.

[0064] On the 32nd day of operation, the total nitrogen concentration of the influent was 940 mg / L. The proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.3% through metagenomic microbial classification sequencing, indicating that enrichment culture was completed. During the culture process, the membrane pressure of the membrane module reached 0.041 MPa on the 24th day, and it was replaced and cleaned.

[0065] Comparative Example 2

[0066] The same as Example 1, except that the gas produced during the culturing process of the anaerobic membrane bioreactor was not collected, and only the same amount of hydrogen was introduced.

[0067] On the 33rd day, the total nitrogen concentration of the influent was 910 mg / L. The metagenomic microbial classification sequencing showed that the proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.2%, indicating that the enrichment culture was completed. During the culture process, the membrane pressure of the membrane module reached 0.041 MPa on the 23rd day, and it was replaced and cleaned.

[0068] Comparative Example 3

[0069] The same as Example 1, except that: the gas generated during the culture process is collected and introduced into the anaerobic membrane bioreactor, and no hydrogen is used.

[0070] On the 37th day of operation, the total nitrogen concentration of the influent was 850 mg / L. The metagenomic microbial classification sequencing showed that the proportion of anaerobic ammonia-oxidizing bacteria in the sludge reached 18.2%, indicating that the enrichment culture was completed. During the culture process, the membrane pressure of the membrane module reached 0.041 MPa on the 22nd day, and it was replaced and cleaned.

Claims

1. A method for enriching and culturing anaerobic ammonia-oxidizing bacteria, characterized in that The method includes the following contents: inoculating activated sludge containing anaerobic ammonia-oxidizing bacteria into an anaerobic membrane bioreactor, starting water inlet, carrying out enrichment culture, collecting gas generated during the culture process, mixing the gas with hydrogen and then introducing the gas into the reactor to carry out enrichment culture of the anaerobic ammonia-oxidizing bacteria; arranging a gas collection system on the top of the anaerobic membrane bioreactor to collect the gas generated during the enrichment culture process; The collected gas is mixed with hydrogen before entering the anaerobic membrane bioreactor, with the volume proportion of hydrogen being 90%-95%.

2. The method according to claim 1, characterized in that: The activated sludge containing anaerobic ammonium oxidizing bacteria is taken from activated sludge or surplus activated sludge from industrial and urban sewage treatment plants, and contains denitrifying bacteria, decarbonizing bacteria and dephosphorizing bacteria, wherein the denitrifying bacteria include anaerobic ammonium oxidizing bacteria, aerobic ammonium oxidizing bacteria and denitrifying bacteria.

3. The method according to claim 1 or 2, characterized in that: The activated sludge is inoculated at a sludge concentration of 4000-6000 mg / L after inoculation.

4. The method according to claim 1, characterized in that: The anaerobic membrane bioreactor is an MBR membrane bioreactor, and the membrane component is a flat membrane or a hollow fiber membrane.

5. The method according to claim 1, characterized in that: The influent contains ammonia nitrogen, nitrite nitrogen and inorganic carbon, wherein the total nitrogen concentration is controlled at 200-1500 mg / L, and the ratio of ammonia nitrogen to nitrite nitrogen is regulated at 1:1.2-1.

3.

6. The method according to claim 5, characterized in that: The substance that provides ammonia nitrogen is ammonium salt.

7. The method according to claim 6, characterized in that: The substance providing ammonia nitrogen is at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate, and ammonium carbonate.

8. The method according to claim 5, characterized in that: The substance that provides nitrite is nitrite.

9. The method according to claim 8, characterized in that: The substance providing nitrite nitrogen is at least one of sodium nitrite and potassium nitrite.

10. The method according to claim 5, characterized in that: The substance providing inorganic carbon is one or more of KHCO3 and NaHCO3, and the concentration of inorganic carbon is controlled at 100-150 mg / L.

11. The method according to claim 1 or 5, characterized in that: Add nutrient solution into the inlet water with the formula: 0.02-0.025g / L KH2PO4, 0.2-0.3g / L CaCl2·2H2O, 0.1-0.2g / L MgSO4·7H2O, 0.005-0.00625g / LFeSO4, 0.005-0.00625g / L EDTA.

12. The method according to claim 1, characterized in that: The enrichment culture conditions are: dissolved oxygen concentration is lower than 0.1 mg / L, temperature is 20-35° C., and pH is 7.0-8.

0.

13. The method according to claim 1, characterized in that: The amount of gas introduced during the entire culture process is controlled by aeration intensity at 5-15m 3 / (m 2 h).

14. The method according to claim 13, characterized in that: The amount of gas introduced during the entire culture process is controlled by aeration intensity at 8-12 m 3 / (m 2 h).

15. The method according to claim 1, characterized in that: During the cultivation process, the inlet substrate concentration is increased in a gradient manner according to the effluent nitrite nitrogen concentration. That is, when the system effluent nitrite nitrogen concentration is lower than 20 mg / L, the inlet total nitrogen concentration is increased by 50-100 mg / L. When the proportion of anaerobic ammonia-oxidizing bacteria in the system increases by more than 1 times, the enrichment culture is completed.

16. The method according to claim 1 or 15, characterized in that: The cultivation process is divided into two stages. In the first stage, the activator is added. When the total nitrogen volume load of the system reaches 1.0 kgN / m 3 / d or more, the first stage of cultivation is completed, the addition of activator is stopped, and the second stage of cultivation begins. When the proportion of anaerobic ammonia-oxidizing bacteria in the system increases by more than 1 times, the enrichment cultivation is completed.

17. The method according to claim 16, characterized in that: The activator is at least one of hydroxylamine, hydrazine and homoserine lactone, and the added concentration is 1-80 mg / L.

18. The method according to claim 1, characterized in that: During the entire cultivation process, the pressure of the anaerobic membrane bioreactor was regulated to be lower than 0.16MPa.

19. A device for the enrichment and cultivation method of anaerobic ammonia-oxidizing bacteria as described in any one of claims 1 to 18, mainly comprising a feeding system, an anaerobic membrane bioreactor, a gas collection system, and a pressure control system; wherein the feeding system is used to regulate the influent ratio and transport the influent to the anaerobic membrane bioreactor; the anaerobic membrane bioreactor is used for the enrichment and cultivation of anaerobic ammonia-oxidizing bacteria; the gas collection system is used to collect the gas generated during the cultivation process and transport it to the anaerobic membrane bioreactor after mixing with hydrogen; the pressure control system is used to regulate the pressure of the membrane bioreactor.

20. The device according to claim 19, characterized in that: The gas collection system includes any one of a gas cabinet, a buffer tank, and a gas collection bag with a gas collection function.

21. The device according to claim 19, characterized in that: The gas collection system further includes a drying device. The collected gas enters the gas storage facility after drying, and is then mixed with hydrogen and transported to the anaerobic membrane bioreactor.

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