Electrophoresis device for processing nucleic acid samples and automatic electrophoresis comb

Abstract

The present invention belongs to the technical field of electrophoresis equipment, and specifically relates to an electrophoresis device for processing nucleic acid samples and an automatic electrophoresis comb. The automatic electrophoresis comb includes a card plate, which has a horizontal frame and a vertical connecting frame, the vertical connecting frame is connected to the horizontal frame, and the connecting frame is used to connect to the electrophoresis tank; a plurality of comb plates, horizontal rods, and gears are provided in the horizontal frame, each gear is installed with a driving device, and the side wall of the horizontal frame is also provided with a controller and a control panel. The electrophoresis device includes an electrophoresis tank, an electrophoresis instrument power supply and a horizontal tank, and also includes an automatic electrophoresis comb. The automatic electrophoresis comb structure designed by the present invention has a variety of shapes, is flexible, has high adaptability, and only has one device, which is easy to store, not easy to lose, and not easy to waste resources; the specification adjustment of the electrophoresis comb is automated and human-machine interactive, so the accuracy of the electrophoresis results is guaranteed after the manufactured gel plate and the electrophoresis device are electrophoresed.

Description

Technical Field

[0001] The invention belongs to the technical field of electrophoresis equipment, and in particular relates to an electrophoresis device for processing nucleic acid samples and an automatic electrophoresis comb. Background Art

[0002] The movement of charged particles towards the electrode with opposite electrical properties under the action of an electric field is called electrophoresis (EP). The technology that uses the different speeds of charged particles moving in an electric field to achieve separation is called electrophoresis. Under certain conditions, the distance that a charged particle moves per unit time (i.e., mobility) is a constant, which is a physicochemical characteristic constant of the charged particle. Different charged particles with different charges, or with the same charge but different charge-to-mass ratios, will separate from each other after a certain period of time due to different movement distances when electrophoresed in the same electric field. The separation distance is proportional to the voltage of the external electric field and the electrophoresis time. Based on this principle, electrophoresis devices came into being.

[0003] Nucleic acid electrophoresis technology mainly includes denaturing gradient gel electrophoresis and agarose electrophoresis. These two electrophoresis are the most commonly used techniques for separating nucleic acid samples. Genes of different fragment lengths have different migration rates, so under the same voltage and the same electrophoresis time, genes of different fragment lengths are migrated to different positions, ultimately achieving the effect of distinguishing nucleic acid samples of different sizes. Combined with downstream steps such as gel cutting, nucleic acid sample recovery, and gene sequencing, nucleic acid samples can be analyzed in detail.

[0004] The commonly used electrophoresis experimental equipment is the electrophoresis device, which mainly includes the electrophoresis instrument power supply and electrophoresis tank, see Figure 1The electrophoresis instrument power supply provides direct current to generate an electric field in the electrophoresis tank to drive the migration of charged molecules. The electrophoresis tank is horizontal, and the electrophoresis buffer is placed in it. Then a horizontal tank with gel is placed. When making the gel, an electrophoresis comb needs to be inserted into the horizontal tank before the gel solidifies. After the gel solidifies, the electrophoresis comb is pulled out to leave a slot for adding nucleic acid samples. The electrophoresis comb in the prior art is a simple comb shape, including a plurality of comb teeth arranged at intervals, and the position of each comb tooth can make a slot on the gel for adding nucleic acid samples. However, the positions between these comb teeth are fixed, and the number of comb teeth is also fixed and cannot be adjusted. In order to overcome the problem that the size of the electrophoresis comb cannot be adjusted, the prior art has prepared electrophoresis combs of various specifications. For example, BIO-RAD has produced electrophoresis combs of various specifications such as CM-075-10, CM-100-10, CM-150-10, CM-075-15, etc. The company uses the advantages of the number of specifications of the electrophoresis comb to meet the needs of different specifications. However, the more things there are, the more difficult it is to preserve, the easier it is to lose, and waste resources. If you buy an electrophoresis comb again, it will be difficult to get the goods in place, which may affect the progress of the experiment and waste resources. In order to solve the problem that the distance between the teeth of the electrophoresis comb cannot be adjusted, the present application has developed an automatically adjustable electrophoresis comb. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides an electrophoresis device and an automatic electrophoresis comb for processing nucleic acid samples.

[0006] The object of the present invention is to provide an automatic electrophoresis comb, comprising a card plate, the card plate having a horizontal frame and at least two vertical connecting frames, the vertical connecting frames being fixedly connected to the horizontal frame, and the connecting frames being used to be connected to an electrophoresis tank;

[0007] The horizontal frame has a cavity inside, and a plurality of comb plates are movably connected to the horizontal frame up and down. A plurality of horizontal rods are arranged in the cavity of the horizontal frame, and a gear is vertically and rotatably arranged on each of the horizontal rods. The positions of the comb plates and the gears correspond one to one, and a driving device is installed on each of the gears. Each of the horizontal rods is connected to the side wall of the cavity of the horizontal frame through a cross bar.

[0008] The side wall of the horizontal frame is also provided with a controller and a control panel. The side of each comb plate is provided with a sensor for sensing the position of the comb plate. All the sensors, driving devices and control panels are electrically connected to the controller.

[0009] Preferably, in the above-mentioned automatic electrophoresis comb, the upper surface of the horizontal frame is provided with top through holes, and the lower surface of the horizontal frame, located directly below the top through holes, is provided with bottom through holes in a one-to-one correspondence, and the comb plate is connected between each group of corresponding top through holes and bottom through holes.

[0010] Preferably, in the above-mentioned automatic electrophoresis comb, each of the gears is meshedly connected to a comb plate, the upper portion of the comb plate is provided with a plurality of tooth-shaped protrusions meshingly connected to the gears, and the lower portion of the comb plate is a flat plate for inserting into the electrophoresis tank.

[0011] Preferably, the above-mentioned automatic electrophoresis comb has a control panel provided with numeric buttons, a distance button, a start button and a reset button, the numeric buttons being used to control the number of the comb plates whose positions need to be adjusted, the distance button being used to control the spacing distance between adjacent comb plates, the start button being used to start the deformation of the automatic comb teeth, and the reset button being used to reset the automatic electrophoresis comb to its initial position.

[0012] Preferably, in the above-mentioned automatic electrophoresis comb, each comb plate is provided with two sensors, one of which is used to sense the position of the comb plate in the reset state; and the other is used to sense the position of the comb plate in the adjustment state, so that the bottom surfaces of all the lowered comb plates are on the same horizontal line.

[0013] Preferably, in the above-mentioned automatic electrophoresis comb, a sliding bar is provided on the surface of the comb plate facing away from the gear, and a sliding groove is provided on the surfaces of the top through-hole and the bottom through-hole adjacent to the sliding bar for matching and sliding connection with the sliding bar.

[0014] Preferably, in the above-mentioned automatic electrophoresis comb, the comb plate includes a toothed plate, a compressible plate and a flat plate fixedly connected in sequence, the toothed plate is provided with the toothed protrusion on the surface adjacent to the gear, which is meshingly connected to the gear, the sliding bar is arranged on the flat plate, and a lateral adjustment device for adjusting the position of the comb plate is provided at the gear.

[0015] Preferably, in the above-mentioned automatic electrophoresis comb, the crossbar of the lateral adjustment device is a horizontally arranged electric telescopic rod, and the electric telescopic rod is electrically connected to the controller;

[0016] Alternatively, the lateral adjustment device is a gear eccentrically connected to the horizontal rod.

[0017] The present invention also provides an electrophoresis device for processing nucleic acid samples, comprising an electrophoresis tank, an electrophoresis instrument power supply and a horizontal tank, and the automatic electrophoresis comb;

[0018] The electrophoresis tank is electrically connected to a power supply of the electrophoresis instrument, the horizontal tank is located in the electrophoresis tank, and the automatic electrophoresis comb is detachably mounted on the electrophoresis tank and inserted into the horizontal tank.

[0019] Preferably, in the electrophoresis device for processing nucleic acid samples, a partition plate is provided in the horizontal groove, a suction strip is provided at the bottom of the partition plate, and a hanging ear is provided at the top of the partition plate, and the hanging ear is hung at the upper edge of the horizontal groove.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The automatic electrophoresis comb structure designed by the present invention can set the specifications of the electrophoresis comb according to the needs of the experimenter. It has a variety of shapes, is flexible, and has high adaptability. There is only one device, which is easy to store, not easy to lose, and not easy to waste resources. In addition, the specification adjustment and insertion of the electrophoresis comb are human-machine interactive operations. There is no need to touch the insertion and handling of the comb by hand, which causes less damage to the gel and is not easy to contaminate the gel, and then it is not easy to contaminate the nucleic acid sample, ensuring the accuracy of the electrophoresis results and facilitating the separation and sequencing of nucleic acid fragments in subsequent experiments.

[0022] 2. In one embodiment of the present invention, the width of the comb plate is adjustable, thereby realizing the function of automatically controlling the size of the nucleic acid loading slot.

[0023] 3. In one embodiment of the present invention, a partition plate is used to divide the horizontal groove into a plurality of areas, and the gel liquid is poured into a specific area, thereby saving the amount of gel liquid, and being energy-saving and environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a structural diagram of an electrophoresis device in the prior art.

[0025] Figure 2 This is the three-dimensional structure of the automatic electrophoresis comb of Example 1.

[0026] Figure 3 Schematic diagram of the connection relationship between the automatic electrophoresis comb and the horizontal electrophoresis tank of Example 1.

[0027] Figure 4 This is a diagram of the internal structure of the automatic electrophoresis comb of Example 1.

[0028] Figure 5 This is a longitudinal cross-sectional view of the automatic electrophoresis comb of Example 1 connected to the horizontal tank.

[0029] Figure 6 This is the module connection diagram of Example 1.

[0030] Figure 7 This is a longitudinal cross-sectional view of the automatic electrophoresis comb of Example 4 connected to the horizontal tank.

[0031] Figure 8 This is a longitudinal cross-sectional view of the automatic electrophoresis comb of Example 5 connected to the horizontal tank.

[0032] Fig. 9 This is a three-dimensional structural diagram of the horizontal groove of Example 6. DETAILED DESCRIPTION

[0033] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0034] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0035] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features; in the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0036] Example 1

[0037] The electrophoresis device structure of the prior art is shown in Figure 1 , including an electrophoresis tank 1 and an electrophoresis instrument power supply 2. The horizontal electrophoresis tank 1 is electrically connected to the electrophoresis instrument power supply 2. The electrophoresis instrument power supply 2 is used to provide current. Some commercially available models of electrophoresis instrument power supplies 2 have functions such as displaying voltage size and electrophoresis time. The electrophoresis tank 1 is also provided with a horizontal tank 11 for making gel, such as the horizontal electrophoresis tank under the American Bio-Rad electrophoresis instrument brand and the electrophoresis instrument power supply and horizontal tank used in conjunction with the horizontal electrophoresis tank.

[0038] This embodiment provides an automatic electrophoresis comb, which can be used in conjunction with the electrophoresis tank 1. The three-dimensional structure of the automatic electrophoresis comb is shown in FIG. Figure 2 The automatic electrophoresis comb includes a card plate 3, which is a U-shaped structure, and has a horizontal frame 31 and two vertical connecting frames 32. The two vertical connecting frames 32 are respectively fixedly connected to the two ends of the horizontal frame 31. A fixing clip is provided on the vertical connecting frame 32. The fixing clip is used to clamp the upper edge of the side of the electrophoresis tank 1. Figure 3The card plate 3 is installed on the electrophoresis tank 1 according to the positional relationship.

[0039] The internal structure of the automatic electrophoresis comb can be found in Figure 4-5 , module connection diagram refer to Figure 6 The interior of the horizontal frame 31 has a cavity. The upper surface of the horizontal frame 31 is provided with a top through hole 311. The lower surface of the horizontal frame 31, located directly below the top through hole 311, is provided with a bottom through hole 312 in a one-to-one correspondence. Each group of corresponding top through holes 311 and bottom through holes 312 form a pair of through holes. Each group of pairs of through holes is used to penetrate a vertical comb plate 4, so that the comb plate 4 is vertically inserted into the horizontal gel liquid in the horizontal groove 11. After the gel liquid solidifies, a vertical nucleic acid loading slot for adding nucleic acid samples can be formed. A plurality of horizontal rods 5 are provided in the cavity of the horizontal frame 31. The horizontal rods 5 are arranged in a horizontal direction. In order to ensure that the nucleic acid loading slot finally made is in a horizontal state, a gear 51 is vertically and rotatably provided on a horizontal rod 5. The center point of the gear 51 is installed on the corresponding horizontal rod 5. The position corresponding to the gear 51 on the horizontal rod 5 is also provided with a driving device for controlling the rotation of the corresponding gear 51. The driving device is a bidirectional rotating motor, such as Figure 4 The bidirectional rotating motor and the horizontal rod 5 are distributed on the opposite sides of the gear 51. A support base is provided at the bottom of the bidirectional rotating motor, which is installed on the bottom wall of the cavity of the horizontal frame 31. The output shaft of the bidirectional rotating motor is connected to the gear 51, and the gear 51 is rotatably connected to the horizontal rod 5. One end of a cross bar 45 is fixed to the side of each horizontal rod 5, and the other end of the cross bar 45 is fixedly connected to the side wall of the cavity of the horizontal frame 31. That is, a cross bar 45, a horizontal rod 5 and a driving device on the horizontal rod 5, a comb plate 4 and a sensor 41 on the comb plate 4 are a group of structures for adjusting the position of the comb plate 4.

[0040] The corresponding gear 51 is controlled to rotate by a driving device, and each gear 51 is meshed with a comb plate 4. A plurality of tooth-shaped protrusions are provided on the upper part of the comb plate 4 near the corresponding gear 51, so that a tooth plate structure is formed on the upper part of the comb plate 4. The tooth plate structure is meshed with the gear 51, and the rotation of the gear 51 is used to drive the tooth plate structure to move up and down, thereby driving the comb plate 4 to move up and down. The lower part of the comb plate 4 is a flat plate, which is used to be inserted into the gel liquid of the horizontal groove 11 to make a nucleic acid loading slot.

[0041] The side wall of the horizontal frame 31 is also provided with a controller and a control panel. The controller adopts an SM series controller. The side of each comb plate 4 is provided with a sensor 41 for sensing the position of the comb plate 4. The sensor 41 adopts a pressure sensor or a position sensor. All sensors 41, drive devices, and control panels are electrically connected to the controller. The controller is also electrically connected to a controller power supply for powering it.

[0042] After the automatic electrophoresis comb of this embodiment is produced, the bottom surfaces of all the comb plates 4 are located on the same horizontal line, ensuring that the operation of adjusting the position of each comb plate 4 can be carried out synchronously, and after adjustment, the bottom surfaces of the comb plates 4 used to make the slots for nucleic acid loading are still located in the same plane; the control panel is provided with digital buttons, distance buttons, start buttons and reset buttons, the digital buttons correspond to the number of comb plates 4, and by pressing the corresponding digital buttons, the comb plates 4 representing the corresponding number of numbers are used to make the slots for nucleic acid loading, the distance button is used to control the spacing distance between adjacent comb plates 4, if the distance button is in the first gear, the spacing distance between adjacent comb plates 4 is the width of one comb plate 4, if the distance button is in the second gear, the spacing distance between adjacent comb plates 4 is the width of two comb plates 4, and so on, the spacing distance between adjacent comb plates 4 can be adjusted by the distance button, the start button is turned on after the comb tooth specifications are set, and then the automatic deformation of the automatic comb teeth is controlled by the controller, and the reset button is used to automatically reset the automatic electrophoresis comb to the factory state after the nucleic acid loading slots are made, so as to facilitate the next use.

[0043] The use process of the automatic electrophoresis comb of the present embodiment is as follows: a program can be pre-written in the controller so that the controller power is turned on, the control panel, the drive device and the sensor 41 enter the standby mode, and the electrophoresis buffer is not added to the electrophoresis tank 1 first, and the liquid gel is first added to the horizontal tank 11, and the horizontal tank 11 is placed in the electrophoresis tank 1, and the card board 3 is installed on the electrophoresis tank 1. According to the number of nucleic acid samples to be electrophoresed and the experimental requirements, the corresponding number buttons and distance buttons on the control panel are pressed to lock a comb tooth of a suitable specification; then the start button is pressed, and the controller controls the corresponding drive device 11 to lock the comb teeth of a suitable specification; Work, at the same time, the sensor tests the position of the comb plate 4, and when the signal fed back by the sensor to the controller indicates that the comb plate 4 must have reached the target position, the controller controls the drive device to stop working. It should be noted that the target position of the comb plate 4 refers to the bottom of the comb plate 4 being inserted into the liquid gel in the horizontal groove 11; the next step is to wait for the formation of the nucleic acid loading slot. Taking agarose gel as an example, it is a liquid in the heated state, and after a period of cooling, it forms a solid. Since the comb plate 4 is inserted into the liquid gel in advance, after solidification, the position where the comb plate 4 is inserted will form a nucleic acid loading slot. Finally, press the reset button on the control panel, and the controller controls the drive device to work again, so that the comb plate 4 is reset to the factory state. After the nucleic acid loading slot is made, turn off the controller power supply, remove the automatic electrophoresis comb, introduce the electrophoresis buffer into the electrophoresis tank 1, connect the electrophoresis tank 1 and the electrophoresis instrument power supply 2, add the nucleic acid sample into the nucleic acid loading slot, turn on the electrophoresis instrument power supply 2, and the electrophoresis work begins.

[0044] It should be noted that the height of the comb plate 4 is designed to match the height of the electrophoresis tank 1 and the horizontal tank 11 to ensure that after the card plate 3 is installed on the electrophoresis tank 1 and before adjustment, that is, in the factory state, the bottom surface of the comb plate 4 does not contact the liquid gel in the horizontal tank 11.

[0045] The above working process can set the specifications of the electrophoresis comb according to the needs of the experimenter. The shape is changeable, flexible, and highly adaptable. There is only one device, which is easy to store, not easy to lose, and not easy to waste resources. In addition, the specification adjustment of the electrophoresis comb is automatic and human-machine interactive. There is no need to touch the comb by hand to insert and hold the comb, which causes less damage to the gel and is not easy to contaminate the gel, and then it is not easy to contaminate the nucleic acid sample, ensuring the accuracy of the electrophoresis results and facilitating the separation and sequencing of nucleic acid fragments in subsequent experiments.

[0046] Example 2

[0047] An automatic electrophoresis comb has a structure that is basically the same as that of the automatic electrophoresis comb in Example 1. However, in order to facilitate the control of the position of the comb tooth plate 4, in this embodiment, two sensors 41 are provided on each comb tooth plate 4, which are named as an upper sensor and a lower sensor respectively. The upper sensor is used to sense the position of the comb tooth plate 4 in a reset state to ensure that the factory position is reached each time the comb tooth plate 4 is reset. The lower sensor is used to sense the position of the comb tooth plate 4 in an adjustment state to ensure that the position of the comb tooth plate 4 is accurate each time it is lowered, and to ensure that the bottom surfaces of all lowered comb tooth plates 4 are on the same horizontal line.

[0048] A convex plate is provided on the surface of the comb plate 4 that is not connected to the gear 51, an upper sensor is provided on the upper surface of the convex plate, and a lower sensor is provided on the lower surface of the convex plate. Both the upper sensor and the lower sensor are pressure sensors. When the upper sensor contacts the top surface inside the horizontal frame 31, it is the factory position, that is, the end position required for resetting. When the lower sensor contacts the bottom surface inside the cavity of the horizontal frame 31, it is the end point of the descent of the comb plate 4.

[0049] Example 3

[0050] An automatic electrophoresis comb has a structure that is basically the same as that of the automatic electrophoresis comb in Example 1. A sliding bar is provided on the surface of the comb plate 4 that is away from the gear 51, and a sliding groove is provided on the surfaces of the top through hole 311 and the bottom through hole 312 that are adjacent to the sliding bar. When the height of the comb plate 4 is adjusted using a controller, the sliding bar and the sliding groove have a sliding guiding function to facilitate the movement of the comb plate 4.

[0051] Example 4

[0052] An automatic electrophoresis comb has a structure substantially the same as that of the automatic electrophoresis comb of Embodiment 3, and also includes a card plate 3, a comb plate 4, a horizontal rod 5, a gear 51, a slide bar, a slide groove, etc. The difference between Embodiment 2 and Embodiment 3 is that:

[0053] The width of the comb plate 4 is adjustable, see Figure 7 , the comb plate 4 includes a toothed plate 42, a compressible plate 43 and a flat plate 44 which are fixedly connected in sequence. The toothed plate 42 is provided with a toothed protrusion on the surface adjacent to the gear 51, which is meshed and connected with the gear 51. The compressible plate 43 is an elastic material that does not react with the gel and is compressible, such as rubber or sponge. The slide bar is arranged on the flat plate 44, and a lateral adjustment device for adjusting its displacement is arranged at the gear 51. The lateral adjustment device can cause the gear 51 to be horizontally displaced, so that the gear 51 moves in the direction of the toothed plate 42, thereby squeezing the compressible plate 43, shortening the width of the comb plate 4, and then adjusting the size of the nucleic acid loading slot made by the comb plate 4, so as to control the amount of nucleic acid loading. The lateral adjustment device provided in this embodiment, in conjunction with the working principle of embodiment 3, realizes the function of automatically controlling the size of the nucleic acid loading slot.

[0054] The lateral adjustment device is a crossbar 45, which is a horizontally arranged electric telescopic rod. The electric telescopic rod is electrically connected to the controller. Under this structure, a width adjustment key is provided on the control panel. When the width adjustment key is pressed, the controller controls the electric telescopic rod to extend, so that the compressible plate 43 is compressed, which is similar to the working principle of the distance key. If the width adjustment key is in the first gear, the compression plate 43 is compressed by 0.2 mm. If the distance key is in the second gear, the compression plate 43 is compressed by 0.4 mm. By analogy, the width of the compressible plate 43 can be adjusted by operating the width adjustment key.

[0055] Preferably, an elastic ring 46 is sleeved on the bottom of the comb plate 4. The elastic ring 46 is a rubber sleeve, which is sleeved on the outside of the bottom of the comb plate 4 composed of the toothed plate 42, the compressible plate 43 and the flat plate 44. The position of the elastic ring 46 is used to insert into the gel liquid. The elasticity of the elastic ring 46 is less than the elasticity of the compressible plate 43. Therefore, when the width of the comb plate 4 is reduced, the compressible plate 43 will not protrude outside the edges of the toothed plate 42 and the flat plate 44 when it is compressed.

[0056] Example 5

[0057] An automatic electrophoresis comb, which has a structure substantially the same as that of the automatic electrophoresis comb of Example 4, except that: the crossbar 45 is not an electric telescopic rod, and the lateral adjustment device is not an electric telescopic rod, but a gear 51 eccentrically connected to the horizontal rod 5, that is, the horizontal rod 5 is not connected to the center of the gear 51, but is connected to a non-center position, and in the factory state, the connection between the two is located directly below the center of the gear 51, then the drive device controls the gear 51 according to Figure 8 When the tooth plate 4 is rotated clockwise as shown in FIG. 1 , on the one hand, the comb plate 4 moves downward under the meshing action, and on the other hand, the tooth plate 42 moves to the left under the action of the eccentric connection. Figure 8 The drive device controls the gear 51 to move in the right direction, compressing the compressible plate 43, and finally reducing the size of the comb plate 4. Figure 8 When the tooth plate 4 is rotated counterclockwise as shown in FIG. 1 , on the one hand, the comb plate 4 moves upward under the meshing action, and on the other hand, the tooth plate 42 moves to the left under the action of the eccentric connection. Figure 8 The leftward movement compresses the compressible plate 43, and finally increases the size of the comb plate 4.

[0058] The lateral adjustment device of this embodiment does not need to be provided with an electric telescopic rod, nor does it need to be provided with a width adjustment button, so the operation is simplified and energy-saving.

[0059] Example 6

[0060] An electrophoresis device for processing nucleic acid samples, comprising the structure of the automatic electrophoresis comb of Example 1, and also comprising the electrophoresis tank 1, the electrophoresis power supply 2, and the horizontal tank 11 described in Example 1: Fig. 9 , a partition plate 6 is provided in the horizontal groove 11, a suction strip 61 is provided at the bottom of the partition plate 6, the suction strip 61 is made of rubber or other materials capable of sealing liquid, and hanging ears 62 are provided at both ends of the top of the partition plate 6, and the hanging ears 62 can be hung at the upper edge of the horizontal groove 11. When the amount of nucleic acid sample to be electrophoresed is small, the partition plate 6 is used to divide the horizontal groove 11 into multiple areas, and the gel solution is poured into a specific area, which can save the amount of gel solution and is environmentally friendly.

[0061] It should be noted that the component connection relationships not specifically mentioned in the present invention are assumed to adopt the existing technology. Since they do not involve the invention point and are widely used in the existing technology, the structural connection relationships are not described in detail.

[0062] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes a preferred embodiment. Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they know the basic creative concept. Therefore, the attached claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0063] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An automatic electrophoresis comb, characterized in that: Comprising a card board (3), the card board (3) having a horizontal frame (31) and at least two vertical connecting frames (32), the vertical connecting frames (32) being fixedly connected to the horizontal frame (31), and the connecting frames (32) being used to be connected to the electrophoresis tank (1); The horizontal frame (31) has a cavity inside, and a plurality of comb plates (4) are movably connected to the horizontal frame (31) up and down. A plurality of horizontal rods (5) are arranged in the cavity of the horizontal frame (31), and a gear (51) is vertically and rotatably arranged on each of the horizontal rods (5). The positions of the comb plates (4) and the gears (51) correspond one to one, and a driving device is installed on each of the gears (51). Each of the horizontal rods (5) is connected to the side wall of the cavity of the horizontal frame (31) via a cross bar (45); The side wall of the horizontal frame (31) is also provided with a controller and a control panel, and the side surface of each comb plate (4) is provided with a sensor (41) for sensing the position of the comb plate (4), and all the sensors (41), the driving device, and the control panel are electrically connected to the controller; The upper surface of the horizontal frame (31) is provided with a top through hole (311), and the lower surface of the horizontal frame (31) is provided with bottom through holes (312) corresponding to each other at positions directly below the top through holes (311), and the comb plate (4) is connected between each group of corresponding top through holes (311) and bottom through holes (312).

2. An automatic electrophoresis comb according to claim 1, characterized in that: Each of the gears (51) is meshedly connected to a comb plate (4); a plurality of tooth-shaped protrusions are provided on the upper portion of the comb plate (4) and meshedly connected to the gears (51); and a lower portion of the comb plate (4) is a flat plate and is used to be inserted into the electrophoresis tank (1).

3. An automatic electrophoresis comb according to claim 2, characterized in that: The control panel is provided with numeric keys, a distance key, a start key and a reset key, the numeric keys are used to control the number of the comb plates (4) whose positions need to be adjusted, the distance keys are used to control the spacing between adjacent comb plates (4), the start key is used to start the deformation of the automatic comb teeth, and the reset key is used to reset the automatic electrophoresis comb to an initial position state.

4. An automatic electrophoresis comb according to claim 1, characterized in that: Each of the comb tooth plates (4) is provided with two sensors (41), one of which is used to sense the position of the comb tooth plate (4) in a reset state; and the other is used to sense the position of the comb tooth plate (4) in an adjusted state, and to ensure that the bottom surfaces of all the comb tooth plates (4) in the adjusted positions are on the same horizontal line.

5. An automatic electrophoresis comb according to claim 2, characterized in that: A sliding bar is provided on the surface of the comb plate (4) facing away from the gear (51), and sliding grooves matching and slidably connected to the sliding bar are provided on the surfaces of the top through-hole (311) and the bottom through-hole (312) adjacent to the sliding bar.

6. An automatic electrophoresis comb according to claim 5, characterized in that: The comb plate (4) comprises a toothed plate (42), a compressible plate (43) and a flat plate (44) which are fixedly connected in sequence; the toothed plate (42) is provided with the toothed protrusion on a surface adjacent to the gear (51) and is meshingly connected with the gear (51); the slide bar is arranged on the flat plate (44); and a lateral adjustment device for adjusting the position of the comb plate (4) is provided at the gear (51).

7. The automatic electrophoresis comb according to claim 6, characterized in that: The lateral adjustment device is a crossbar (45), the crossbar (45) is a horizontally arranged electric telescopic rod, and the electric telescopic rod is electrically connected to the controller; Alternatively, the lateral adjustment device is a gear (51) eccentrically connected to the horizontal rod (5).

8. An electrophoresis device for processing nucleic acid samples, characterized in that: It comprises an electrophoresis tank (1), an electrophoresis apparatus power supply (2) and a horizontal tank (11), and also comprises the automatic electrophoresis comb according to any one of claims 1 to 7; The electrophoresis tank (1) is electrically connected to an electrophoresis instrument power supply (2); the horizontal tank (11) is located in the electrophoresis tank (1); and the automatic electrophoresis comb is detachably mounted on the electrophoresis tank (1) and inserted into the horizontal tank (11).

9. The electrophoresis device for processing nucleic acid samples according to claim 8, characterized in that: A partition plate (6) is arranged in the horizontal groove (11), a suction strip (61) is arranged at the bottom of the partition plate (6), and a hanging ear (62) is arranged at the top of the partition plate (6), and the hanging ear (62) is hung at the upper edge of the horizontal groove (11).

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