An invasive detection device and method for leaf living hormone content information

Abstract

The invention relates to an invasive detection device and method for information on the content of living hormones in leaves; the device comprises an invasive living body sampling mechanism and an invasive living body detection mechanism; the invasive living body sampling mechanism comprises a box body and a box cover; a thorn-like protrusion layer of replaceable specifications is installed on the box cover, and a plurality of pyramid-shaped thorn-like protrusions are provided on the thorn-like protrusion layer; a sampling chamber and a detection chamber are arranged in the box body; a connecting hole is provided between the sampling chamber and the detection chamber, and the connecting hole is opened or closed by an opening / closing mechanism; the invasive living body detection mechanism comprises a bracket, a working electrode, an auxiliary electrode and a reference electrode arranged in an installation channel in the bracket; a support is provided at the upper end of the box body; when the bracket is installed in the support, the lower parts of the working electrode, the auxiliary electrode and the reference electrode extend into the detection chamber; the upper parts of the working electrode, the auxiliary electrode and the reference electrode are connected to a portable electrochemical workstation through a signal line.

Description

Technical Field

[0001] The invention relates to the field of electrochemical detection, and in particular to an invasive detection device and method for information on the content of living hormones in leaves. Background Art

[0002] Plant hormones are a general term for a class of biologically active substances with very small molecular weights that are extremely rare in plants. As signal molecules that regulate various life activities of plants, plant hormones are synthesized in different plant organs in the plant body and reach their respective sites of action through different transport methods such as the plant's own polar, non-polar, lateral, and sieve tube transport. They play a vital regulatory role in various stages of plant development, such as seedlings, growth, maturity, and aging. Based on this, capturing, analyzing, and dynamically monitoring plant hormones can help understand the different growth states of plants in each growth cycle, and then regulate and intervene in the growth and development of crops, which will bring huge benefits to agricultural production practices.

[0003] At present, there are many methods for detecting plant hormones. Among them, electrochemical detection technology stands out with its advantages such as portable instruments, relatively low cost, simple use, high sensitivity and good selectivity, and has been widely used. Among them, the three-electrode system is a commonly used detection system in electrochemical detection technology. However, due to the limitations of the material and appearance characteristics of each electrode, the application of this system requires the production of corresponding detection devices. It is difficult to complete the task of in vivo detection using only exposed working, auxiliary and reference electrodes.

[0004] Most common electrochemical detection devices used in invasive detection use simple electrode packages or put a hard shell on the outside of the direct electrode to facilitate connection with a portable electrochemical workstation. They mainly focus on solving the problem of how to place three detection electrodes to achieve effective connection between the detection electrodes and the test workstation to avoid test signal attenuation, but do not specifically consider the characteristics and difficulties of in vivo detection.

[0005] However, the above electrochemical detection device has the following disadvantages:

[0006] (1) Simple connection of detection electrodes or simple shell packaging will lead to frequent problems during the detection process, including but not limited to working electrode breakage, large fluctuations in sampling data, and even inaccurate sampling data;

[0007] (2) The above-mentioned simple connection method has great limitations in achieving invasive detection. First, the hardness of the electrode cannot meet the requirements for invading the scleroderma of plants, and auxiliary tools are needed. Secondly, the thickness of plant leaves is very thin, usually less than 0.5 mm. The electrode will penetrate the leaves, and the effective detection reaction area of ​​the electrode cannot be fixed, resulting in the inability to achieve invasive detection of plant hormones in the leaves. Thirdly, the manual sampling method is to manually puncture the leaves with a needle to leave a needle hole, and then soak the leaves in phosphate buffer to obtain leaf juice. However, the acupuncture method is difficult to ensure the number of effective openings in the leaves (if there are too few openings, the leaching amount is insufficient; if there are too many openings, the damage is too great). At the same time, the circular incision is more susceptible to the surface tension of the liquid, making it difficult for the leaf juice to seep out, so there is a problem of low leaching efficiency. Summary of the invention

[0008] The purpose of the present invention is to solve the problem that existing invasive devices cannot directly complete hormone detection of living tissues of plant leaves, and proposes an invasive detection device for information on the living hormone content of leaves. The invasive detection device can meet the needs of invasive detection of living tissues of key parts of plants, while providing effective protection for electrodes, and limit the invasive damage to plants without affecting the short-term self-healing of plants.

[0009] The second object of the present invention is to provide an invasive detection method for leaf living hormone content information using the above-mentioned invasive detection device for leaf living hormone content information.

[0010] The technical solution of the present invention to solve the above technical problems is:

[0011] An invasive detection device for leaf living body hormone content information includes an invasive living body sampling mechanism for sampling leaves and an invasive living body detection mechanism for detecting the hormone content of the sampled solution, wherein:

[0012] The invasive living body sampling mechanism comprises a box body and a box cover arranged at the opening at the lower end of the box body, wherein:

[0013] The box body and the box cover are detachably connected; a sampling component is arranged on the box cover, and the sampling component is a thorn-shaped protrusion layer, and the thorn-shaped protrusion layer is installed on the box cover, and a plurality of pyramid-shaped thorn-shaped protrusions are arranged on the thorn-shaped protrusion layer;

[0014] The box body is provided with two upper and lower chambers, wherein the lower chamber is a sampling chamber and the upper chamber is a detection chamber; a connecting hole is provided between the sampling chamber and the detection chamber, and the connecting hole is opened or closed by an opening / closing mechanism; wherein the box body is provided with an opening at the lower end of the sampling chamber; when the box body is matched with the box cover, the edge of the thorn-like protrusion sheet layer is in contact with the inside and outside of the opening;

[0015] The invasive living body detection mechanism includes a bracket, a working electrode, an auxiliary electrode and a reference electrode arranged in the bracket; wherein, a support for mounting the bracket is arranged at the upper end of the box body; an installation channel is arranged in the bracket at the corresponding position of the working electrode, the auxiliary electrode and the reference electrode, and the installation channel vertically passes through the upper and lower sides of the bracket; when the bracket is installed in the support, the lower parts of the working electrode, the auxiliary electrode and the reference electrode extend into the detection chamber; the upper parts of the working electrode, the auxiliary electrode and the reference electrode are connected to the portable electrochemical workstation through a signal line.

[0016] Preferably, the bracket is a Venn figure column bracket, and the support is a Venn figure hollow support.

[0017] Preferably, the bracket is provided with a socket in the middle and upper part of the mounting channel; the upper ends of the working electrode, the auxiliary electrode and the reference electrode are respectively embedded in the interior of the socket, and a signal line is welded to the upper part of the socket, and the signal line is electrically connected to the working electrode, auxiliary electrode and reference electrode embedded in the socket, and the signal line is led out from the upper end of the bracket along the mounting channel of the bracket.

[0018] Preferably, the box body and the box cover are fixed by magnet adsorption, wherein a first circular groove is provided on both sides of the box cover, and a circular magnet is provided in the first circular groove; correspondingly, a second circular groove is provided at the lower end of the box body at a position corresponding to the first circular groove, and a circular magnet is also provided in the second circular groove.

[0019] Preferably, the box cover is provided with a bracket, the bracket is located in the middle of the first circular grooves on both sides, the thorn-like protrusion layer is installed on the bracket, and the outer side of the box cover is provided with a circular hole at a position corresponding to the bracket.

[0020] Preferably, five pyramid-shaped thorn-like protrusions are evenly distributed on the upper surface of the thorn-like protrusion layer; and the edge of the thorn-like protrusion layer is coated with crystal glue for waterproofing.

[0021] Preferably, the opening / closing mechanism comprises a circular stopper and a vertical driving mechanism for driving the circular stopper to move vertically to open or block the communicating hole, wherein:

[0022] The box body is provided with a truncated cone on the outer side of the communicating hole, and a circular stepped groove is provided inside the truncated cone. The circular stepped groove is communicated with the communicating hole and cooperates with the circular baffle;

[0023] The vertical driving mechanism comprises a cylinder disposed at the upper end of the box body, a support shaft disposed in the cylinder body, and a vertical power mechanism for driving the support shaft to perform vertical movement, wherein:

[0024] The cylinder is mounted on the inner top wall of the box body; the lower end of the support shaft extends out of the cylinder and is connected to the circular stopper, and the upper end of the support shaft is provided with a connecting seat; a first connecting hole is provided in the connecting seat, and a spring is provided between the connecting seat and the inner top wall of the box body, and the spring is provided in the cylinder, and the two ends of the spring act on the connecting seat and the inner top wall of the box body respectively, and the elastic force of the spring prompts the circular stopper to move downward and press tightly into the circular stepped groove of the truncated table, so as to achieve the blocking of the connecting hole;

[0025] The vertical power mechanism includes a toggle switch and a connecting rope, wherein the toggle switch includes a hinge seat arranged on the upper side of the box body and a toggle rod rotatably connected to the hinge seat, wherein one end of the toggle rod is provided with a second connecting hole, one end of the connecting rope is tied to the second connecting hole, and the other end passes through the opening on the box body and enters into the cylinder body, and is tied to the first connecting hole on the connecting seat in the cylinder body.

[0026] Preferably, the working electrode is a cylindrical electrode with a plastic-sealed middle portion; the auxiliary electrode is a platinum wire electrode with a plastic-sealed middle portion; and the reference electrode is a silver / silver chloride electrode with a plastic-sealed middle portion.

[0027] Preferably, semicircular fixing rings are provided on both sides of the upper end of the box body.

[0028] An invasive detection method for leaf living hormone content information comprises the following steps:

[0029] S1. Install a thorn-shaped protrusion sheet layer of preset specifications on the box cover;

[0030] S2. Clean the tissue part to be tested in the plant leaf with phosphate buffer, then tilt the box body downward so that the lower end of the box body is tilted upward, and then inject 1-2 mL of phosphate buffer into the sampling chamber; then clamp the plant leaf between the box cover and the box body so that the tissue part to be tested in the plant leaf is located within the opening range of the sampling chamber of the box body; finally, cover the box cover so that the thorn-like protrusions in the thorn-like protrusion layer pierce the tissue part to be tested in the plant leaf;

[0031] S3, tying a thin rope to the semicircular fixing rings on both sides of the upper end of the box body, thereby hanging the box body on a branch or stem near the sampled plant leaves, so that the plant leaves are naturally stretched, and waiting for the juice of the plant leaves to diffuse into the phosphate buffer;

[0032] S4. After the sampling is completed, the box body is tilted again so that the lower end of the box body is tilted upward, the box cover is opened to take out the plant leaves and then the box cover is closed; the connecting hole is opened by the opening / closing mechanism so that the solution to be tested in the sampling chamber enters the testing chamber, and then the connecting hole is closed by the opening / closing mechanism, and finally the box body is placed horizontally;

[0033] S5, installing the working electrode, the auxiliary electrode, and the reference electrode on the bracket, then removing the colloid piston on the support at the upper end of the box body, and installing the bracket on the support, so that the working electrode, the auxiliary electrode, and the reference electrode enter the detection chamber of the box body respectively, and contact with the solution to be detected in the detection chamber, and stand still to activate the working electrode;

[0034] S6. Connect the signal line leading out from the upper end of the bracket to a portable electrochemical workstation to obtain target hormone content data in the plant leaves.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] 1. In the invasive detection device for leaf living hormone content information of the present invention, the working electrode, the auxiliary electrode and the reference electrode are installed in the installation channel of the bracket, and are connected to the portable electrochemical workstation by leading out a signal line at the top of the bracket. This can prevent the working electrode, the auxiliary electrode and the reference electrode from contacting each other and other electrical components, thereby providing good protection for the electrodes.

[0037] 2. The invasive detection device for the information of the living hormone content in leaves of the present invention adopts an invasive structure design with replaceable pyramidal thorn-like protrusions, which limits the degree of damage to plant leaves in different growth states to a condition that does not affect their self-healing in a short time; at the same time, it avoids the problem that the previous acupuncture method cannot guarantee the effective number of openings and the circular incision is easily affected by the surface tension of the liquid, resulting in low efficiency of leaf tissue fluid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the three-dimensional structure of the invasive detection device for the information of the living hormone content in leaves of the present invention.

[0039] Figure 2 It is a cross-sectional view of the invasive detection device for leaf living hormone content information of the present invention.

[0040] Figure 3It is a schematic diagram of the three-dimensional structure of the box cover, circular magnet and thorn-like protrusion layer.

[0041] Figure 4 It is a schematic diagram of the three-dimensional structure of the box cover.

[0042] Figure 5 It is a schematic diagram of the three-dimensional structure of the box body.

[0043] Figure 6 A schematic diagram of the three-dimensional structure of the bracket.

[0044] Figure 7 It is a schematic diagram of the three-dimensional structure of the support, toggle switch and cylinder.

[0045] Figure 8 It is a schematic diagram of the three-dimensional structure of the colloid piston. DETAILED DESCRIPTION

[0046] The present invention is further described in detail below in conjunction with embodiments and drawings, but the embodiments of the present invention are not limited thereto.

[0047] See also Figure 1-Figure 8 The invasive detection device for leaf living hormone content information of the present invention includes an invasive living body sampling mechanism for sampling leaves and an invasive living body detection mechanism for detecting hormone content in the sampled solution.

[0048] See also Figure 1-Figure 8 The invasive living body sampling mechanism includes a box body 2 and a box cover 1 arranged at the lower end opening of the box body 2, wherein the box body 2 and the box cover 1 are detachably connected.

[0049] In this embodiment, the box body 2 and the box cover 1 are fixed by magnet adsorption. Both sides of the box cover 1 are provided with a first circular groove 102, and a circular magnet 13 is provided in the first circular groove 102; correspondingly, the lower end of the box body 2 is provided with a second circular groove at a position corresponding to the first circular groove 102, and a circular magnet 13 is also provided in the second circular groove; through the above arrangement, the box cover 1 is installed and fixed on the box body 2 by magnetic attraction. When detection is required, the box cover 1 clamps the plant leaves on the box body 2 so that the position to be sampled in the plant leaves is located at the opening, and the thorn-like protrusion layer 11 on the box cover 1 pierces the position to be sampled in the plant leaves.

[0050] See also Figure 1-Figure 8A sampling component is arranged on the box cover 1, and the sampling component is a pyramid-shaped thorn-like protrusion layer 11, and the thorn-like protrusion layer 11 is installed on the box cover 1. Five pyramid-shaped (i.e. pyramid-shaped) thorn-like protrusions 12 are evenly distributed on the upper surface of the thorn-like protrusion layer 11, and the edge of the thorn-like protrusion layer 11 is coated with crystal glue for waterproofing. In this way, when the box cover 1 is installed on the box body 2, the edge of the thorn-like protrusion layer 11 in the box cover 1 cooperates with the opening (also coated with crystal glue for waterproofing), thereby preventing the solution in the sampling chamber 204 from leaking.

[0051] In this embodiment, there can be multiple groups of the thorn-like protrusion layers 11, and each group of thorn-like protrusion layers 11 has the same size. Although the shapes of their thorn-like protrusions 12 are the same, the sizes and quantities are different. This can be adapted to detecting the hormone content of plant leaves under different growth conditions.

[0052] See also Figure 1-Figure 8 The box cover 1 is provided with a bracket 103, and the bracket 103 is located in the middle of the first circular grooves 102 on both sides. The thorn-like protrusion layer 11 is installed on the bracket 103, and the outer side of the box cover 1 is provided with a circular hole 104 at a position corresponding to the bracket 103; by providing the circular hole 104, it is convenient to replace the thorn-like protrusion layer 11. When replacing, it is only necessary to squeeze the thorn-like protrusion layer 11 inward from the circular hole 104 on the outer side of the box cover 1 so that the thorn-like protrusion layer 11 is removed from the bracket 103, and then the thorn-like protrusion layer 11 of appropriate specifications is re-installed into the bracket 103, so that it can be adapted to detect the hormone content of plant leaves under different growth states.

[0053] See also Figure 1-Figure 8 The box body 2 is provided with two upper and lower chambers, wherein the lower chamber is a sampling chamber 204, and the upper chamber is a detection chamber 202; a connecting hole 203 is provided between the sampling chamber 204 and the detection chamber 202, and the connecting hole 203 is opened or closed by an opening / closing mechanism; wherein the box body 2 is provided with the above-mentioned opening at the lower end of the sampling chamber 204; when the box body 2 is matched with the box cover 1, the edge of the thorn-like protrusion layer 11 is fitted inside and outside the edge of the opening.

[0054] See also Figure 1-Figure 8, the opening / closing mechanism includes a circular stopper 14 and a vertical driving mechanism for driving the circular stopper 14 to move vertically to open or block the communicating hole 203, wherein the box body 2 is provided with a round table 201 on the outer side of the communicating hole 203, and a circular stepped groove is provided in the round table 201, and the circular stepped groove is communicated with the communicating hole 203 and cooperates with the circular baffle 14; the vertical driving mechanism includes a cylinder 10 arranged at the upper end of the box body 2, a support shaft arranged in the cylinder 10, and a vertical power mechanism for driving the support shaft to move vertically, wherein the cylinder 10 is installed on the inner top wall of the box body 2; the lower end of the support shaft extends out of the cylinder 10 and is connected to the circular stopper 14, and the upper end of the support shaft is provided with a connecting seat; the connecting seat is provided with a first connecting hole 15, and the connecting hole 15 is provided in the connecting seat. A spring 11 is arranged between the connecting seat and the inner top wall of the box body 2, and the spring 11 is arranged in the cylinder 10. The two ends of the spring 11 act on the connecting seat and the inner top wall of the box body 2 respectively, and the elastic force of the spring 11 prompts the circular stopper 14 to move downward and press tightly into the circular stepped groove of the truncated table 201 to achieve the blocking of the connecting hole 203; the vertical power mechanism includes a toggle switch and a connecting rope, wherein the toggle switch includes a hinge seat 7 arranged on the upper side of the box body 2 and a lever 8 rotatably connected to the hinge seat 7, wherein one end of the lever 8 is provided with a second connecting hole 16, one end of the connecting rope is tied to the second connecting hole 16, and the other end passes through the opening on the box body 2 and enters into the cylinder 10, and is tied to the first connecting hole 15 on the connecting seat in the cylinder 10.

[0055] Through the above arrangement, when it is necessary to open the connecting hole 203, the lever 8 can be moved, and the lever 8 rotates around the hinge seat 7 to tighten the connecting rope upward, and the connecting rope drives the connecting seat, the support shaft connected to the connecting seat and the circular stopper 14 to overcome the elastic force of the spring 11 and move upward, so that the circular stopper 14 is separated from the circular stepped groove on the truncated table 201, so that the connecting hole 203 is in an open state; when the force on the lever 8 disappears, the spring 11 is reset, thereby driving the connecting seat, the support shaft connected to the connecting seat and the circular stopper 14 to reset, so that the circular stopper 14 cooperates with the circular stepped groove on the truncated table 201 again, so that the connecting hole 203 is in a blocked state; at this time, the lever 8 is also reset. In addition, in order to ensure the sealing effect, sealant or a sealing ring can be provided on the edge of the circular stopper 14; the support shaft can be a stepped shaft, and the part with a larger internal diameter cooperates with the inner cavity of the cylinder 10, thereby forming a vertical sliding mechanism to realize the vertical guidance of the vertical movement of the circular stopper 14; in addition, the bottom of the cylinder 10 cooperates with the support shaft, so that the interior of the cylinder 10 is in a sealed state, which can prevent the liquid to be detected from flowing into the cylinder 10 due to the tilt of the box body 2.

[0056] In this embodiment, the connecting seat, the support shaft and the circular stopper 14 are an integrated structure, and the integrated structure is an inverted T-shape as a whole. The circular baffle 14 is at the bottom, and the support shaft is in the middle. The support shaft cooperates with the inner cavity of the cylinder 10; the connecting seat is at the top, and the outer diameter of the connecting seat is smaller than the outer diameter of the support shaft, and the spring 11 is sleeved on the connecting seat.

[0057] See also Figure 1-Figure 8 The invasive living body detection mechanism includes a bracket 3, a working electrode, an auxiliary electrode and a reference electrode arranged in the bracket 3; wherein, a support 4 for installing the bracket 3 is provided at the upper end of the box body 2, and the bracket 3 is installed in the support 4; an installation channel is provided in the bracket 3 at the corresponding position of the working electrode, the auxiliary electrode and the reference electrode, and the installation channel vertically passes through the upper and lower sides of the bracket 3; when the bracket 3 is installed in the support 4, the lower parts of the working electrode, the auxiliary electrode and the reference electrode extend into the detection chamber 202; the upper parts of the working electrode, the auxiliary electrode and the reference electrode are connected to the portable electrochemical workstation through a signal line.

[0058] In this embodiment, the bracket 3 is a Wien figure column bracket, and the support 4 is a Wien figure hollow support, wherein the outer side of the lower side of the Wien figure hollow support is a three-pronged hollow column shape, the purpose of which is to facilitate the cleaning of the box body, the clean water flowing in from the opening at the lower end of the box body can pass through the hollow part and then flow out from the top of the support, so as to achieve the effect of completely flushing the inside of the box body; the bottom of the lower side of the Wien figure hollow support is provided with a supporting part; the upper part of the Wien figure hollow support is provided with Installation groove, when the Venn diagram column bracket is installed in the installation groove, the bottom of the Venn diagram column bracket is supported on the supporting part, but this does not affect the working electrode, the auxiliary electrode and the reference electrode extending into the detection chamber 202; in addition, the upper part of the Venn diagram hollow support is provided with a colloid piston 17 matched therewith, and the upper and lower ends of the colloid piston 17 are the same Venn diagram-shaped cylinders, the upper end is slightly wider than the lower end, and the upper end fits with the top of the Venn diagram hollow support. Through the above arrangement, when sampling plant leaves, the colloid piston 17 is installed on the upper part of the Venn diagram hollow support, so that the upper end of the box body 2 can be sealed; when the solution needs to be tested, it is only necessary to unplug the colloid piston 17, and then insert the Venn diagram column bracket into the installation groove at the upper end of the Venn diagram hollow support (the cross section of the installation groove is also a Venn diagram), thereby realizing the rapid installation of the invasive liveness detection mechanism.

[0059] See also Figure 1-Figure 8 , the bracket 3 is provided with a holder 5 in the middle and upper part of the installation channel; the upper ends of the working electrode, the auxiliary electrode and the reference electrode are respectively embedded in the inside of the holder 5, and the upper part of the holder 5 is welded with the signal line, wherein the signal line is electrically connected to the working electrode, the auxiliary electrode and the reference electrode embedded in the holder 5, and the signal line is led out from the upper end of the bracket 3 along the installation channel at the upper part of the bracket 3. Through the above arrangement, the working electrode, the auxiliary electrode and the reference electrode can be quickly installed on the bracket 3, and connected to the signal line at the same time. In addition, by adopting the design idea of ​​breaking the whole into parts, all the mechanism components, especially the three electrodes, can be replaced independently; in addition, the design of the holder 5 prevents the electrode from directly contacting the tiger's mouth clamp of the portable electrochemical workstation, providing better protection for the electrode.

[0060] In this embodiment, the self-sealed working electrode, auxiliary electrode, and reference electrode are embedded from the bottom of the installation channel; the upper end of the bracket 3 is provided with three opening grooves of the same shape but different positions, and the card holder 5 is embedded therein, and the top of the card holder 5 is connected to a signal line, and the signal line is led out from the top of the bracket 3 along the installation channel at the upper part of the bracket 3 and connected to the portable electrochemical workstation. In addition, the card holder 5 is a very small rectangular hollow cylinder with a top seal, which is specially made of iron sheet pressing, and the middle half openings on both sides are recessed inward to a certain extent, and the sealing end grows an electrical connection point upward, and the card holder 5 is embedded in the opening groove of the Wien figure column bracket; wherein, the cylindrical working electrode is placed on the side with the highest position of the opening groove, and the auxiliary electrode and the reference electrode are placed in the two opening grooves with the same position height.

[0061] See also Figure 1-Figure 8 The working electrode is a cylindrical electrode of any material with a plastic-sealed middle part, and can be modified; the auxiliary electrode is a platinum wire electrode with a plastic-sealed middle part; the reference electrode is a cylindrical silver / silver chloride electrode with a plastic-sealed middle part; wherein the outer surfaces of the working electrode, the auxiliary electrode and the reference electrode are encapsulated by a heat shrink tube.

[0062] See also Figure 1-Figure 8 Semicircular fixing rings 9 are provided on both sides of the upper end of the box body 2. By providing the semicircular fixing rings 9, the box body 2 can be conveniently suspended during sampling to ensure that the box body 2 is in a vertical state.

[0063] See also Figure 1-Figure 8 The invasive detection method of the leaf living hormone content information of the present invention comprises the following steps:

[0064] S1, installing a thorn-shaped protrusion layer 11 of preset specifications on the box cover 1;

[0065] S2, clean the tissue part to be tested in the plant leaf with phosphate buffer, then tilt the box body 2 downward so that the lower end of the box body 2 is tilted upward, and then inject 1-2mL of phosphate buffer into the sampling chamber 204; then clamp the plant leaf between the box cover 1 and the box body 2 so that the tissue part to be tested in the plant leaf is located within the opening range of the sampling chamber 204 of the box body 2; finally, cover the box cover 1 so that the thorn-like protrusions 12 in the thorn-like protrusion layer 11 pierce the tissue part to be tested in the plant leaf;

[0066] S3, tying a thin rope to the semicircular fixing rings 9 on both sides of the upper end of the box body 2, thereby hanging the box body 2 on the branches or stems near the sampled plant leaves, so that the plant leaves are naturally stretched, and waiting for the juice of the plant leaves to diffuse into the phosphate buffer;

[0067] S4, after the sampling is completed, the box body 2 is tilted again so that the lower end of the box body 2 is tilted upward, the box cover 1 is opened to take out the plant leaves and then the box cover 1 is closed; the connecting hole 203 is opened by the opening / closing mechanism so that the solution to be detected in the sampling chamber 204 enters the detection chamber 202, and then the connecting hole 203 is closed by the opening / closing mechanism, and finally the box body 2 is placed horizontally;

[0068] S5, installing the working electrode, the auxiliary electrode, and the reference electrode on the bracket 3, then removing the colloid piston 17 on the support 4 at the upper end of the box body 2, and installing the bracket 3 on the support 4, so that the working electrode, the auxiliary electrode, and the reference electrode enter the detection chamber 202 of the box body 2 respectively, and contact with the solution to be detected in the detection chamber 202, and stand still to activate the working electrode;

[0069] S6. The signal line drawn out from the upper end of the bracket 3 is connected to a portable electrochemical workstation to obtain target hormone content data in plant leaves.

[0070] In the present invention, the electrochemical sensor system with a three-electrode structure is continued to be used. In view of the problem that the invasive structure cannot directly complete the hormone detection of the living tissue of the plant leaves, an invasive living body sampling mechanism used in conjunction with the invasive living body detection mechanism is designed; the invasive living body sampling mechanism is equipped with a thorn-like protrusion layer 11 of preset specifications (i.e., designed according to the specific growth conditions of the plant leaves), to ensure that the degree of invasive damage caused when dealing with plant leaves in different growth states is controllable, and to limit the damage to the plant leaves under the condition that it does not affect the self-healing of the living plant in a short time; in addition, the corresponding pyramid-shaped (i.e., pyramid-shaped) thorn-like protrusion 12 structure of preset specifications has a regular incision for the invasion of the leaves, a constant number of incisions, and the triangular protrusion incisions produced can also be fully exposed to the phosphate buffer solution, thereby improving the leaching efficiency of the living leaf tissue fluid.

[0071] The above is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited to the above content. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An invasive detection device for leaf living hormone content information, characterized in that: It includes an invasive living body sampling mechanism for sampling leaves and an invasive living body detection mechanism for detecting the hormone content of the sampled solution, wherein: The invasive living body sampling mechanism comprises a box body and a box cover arranged at the opening at the lower end of the box body, wherein: The box body and the box cover are detachably connected; a sampling component is arranged on the box cover, and the sampling component is a thorn-shaped protrusion layer, and the thorn-shaped protrusion layer is installed on the box cover, and a plurality of pyramid-shaped thorn-shaped protrusions are arranged on the thorn-shaped protrusion layer; The box body is provided with two upper and lower chambers, wherein the lower chamber is a sampling chamber and the upper chamber is a detection chamber; a connecting hole is provided between the sampling chamber and the detection chamber, and the connecting hole is opened or closed by an opening / closing mechanism; wherein the box body is provided with an opening at the lower end of the sampling chamber; when the box body is matched with the box cover, the edge of the thorn-like protrusion sheet layer is in contact with the inside and outside of the opening; The invasive living body detection mechanism comprises a bracket, a working electrode, an auxiliary electrode and a reference electrode arranged in the bracket; wherein a support for mounting the bracket is arranged at the upper end of the box body; a mounting channel is arranged in the bracket at positions corresponding to the working electrode, the auxiliary electrode and the reference electrode, and the mounting channel vertically penetrates the upper and lower sides of the bracket; when the bracket is installed in the support, the lower parts of the working electrode, the auxiliary electrode and the reference electrode extend into the detection chamber; the upper parts of the working electrode, the auxiliary electrode and the reference electrode are connected to the portable electrochemical workstation through a signal line; The opening / closing mechanism includes a circular stopper and a vertical driving mechanism for driving the circular stopper to move vertically to open or block the communication hole, wherein: The box body is provided with a truncated cone on the outside of the communicating hole, and a circular stepped groove is provided inside the truncated cone. The circular stepped groove is communicated with the communicating hole and cooperates with the circular stopper; The vertical driving mechanism comprises a cylinder disposed at the upper end of the box body, a support shaft disposed in the cylinder body, and a vertical power mechanism for driving the support shaft to perform vertical movement, wherein: The cylinder is mounted on the inner top wall of the box body; the lower end of the support shaft extends out of the cylinder and is connected to the circular stopper, and the upper end of the support shaft is provided with a connecting seat; a first connecting hole is provided in the connecting seat, and a spring is provided between the connecting seat and the inner top wall of the box body, and the spring is provided in the cylinder, and the two ends of the spring act on the connecting seat and the inner top wall of the box body respectively, and the elastic force of the spring prompts the circular stopper to move downward and press tightly into the circular stepped groove of the truncated table, so as to achieve the blocking of the connecting hole; The vertical power mechanism includes a toggle switch and a connecting rope, wherein the toggle switch includes a hinge seat arranged on the upper side of the box body and a toggle rod rotatably connected to the hinge seat, wherein one end of the toggle rod is provided with a second connecting hole, one end of the connecting rope is tied to the second connecting hole, and the other end passes through the opening on the box body and enters into the cylinder body, and is tied to the first connecting hole on the connecting seat in the cylinder body.

2. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: The bracket is a Venn figure column bracket, and the support is a Venn figure hollow support.

3. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: The bracket is provided with a socket in the middle and upper part of the mounting channel; the upper ends of the working electrode, the auxiliary electrode and the reference electrode are respectively embedded in the interior of the socket, and a signal line is welded on the upper part of the socket. The signal line is electrically connected to the working electrode, the auxiliary electrode and the reference electrode embedded in the socket, and the signal line is led out from the upper end of the bracket along the mounting channel of the bracket.

4. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: The box body and the box cover are fixed by magnet adsorption, wherein a first circular groove is provided on both sides of the box cover, and a circular magnet is provided in the first circular groove; correspondingly, a second circular groove is provided at the lower end of the box body at a position corresponding to the first circular groove, and a circular magnet is also provided in the second circular groove.

5. The invasive detection device for leaf living hormone content information according to claim 4, characterized in that: The box cover is provided with a bracket, the bracket is located in the middle of the first circular grooves on both sides, the thorn-shaped protrusion layer is installed on the bracket, and the outer side of the box cover is provided with a circular hole at a position corresponding to the bracket.

6. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: Five pyramid-shaped thorn-shaped protrusions are evenly distributed on the upper surface of the thorn-shaped protrusion layer; the edge of the thorn-shaped protrusion layer is coated with crystal glue for waterproofing.

7. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: The working electrode is a cylindrical electrode with a plastic-sealed middle portion; the auxiliary electrode is a platinum wire electrode with a plastic-sealed middle portion; and the reference electrode is a silver / silver chloride electrode with a plastic-sealed middle portion.

8. The invasive detection device for leaf living hormone content information according to claim 1, characterized in that: Semicircular fixing rings are arranged on both sides of the upper end of the box body.

9. An invasive detection method for leaf living hormone content information using the invasive detection device for leaf living hormone content information according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Install a thorn-shaped protrusion sheet layer of preset specifications on the box cover; S2. Clean the tissue part to be tested in the plant leaf with phosphate buffer, then tilt the box body downward so that the lower end of the box body is tilted upward, and then inject 1-2 mL of phosphate buffer into the sampling chamber; then clamp the plant leaf between the box cover and the box body so that the tissue part to be tested in the plant leaf is located within the opening range of the sampling chamber of the box body; finally, cover the box cover so that the thorn-like protrusions in the thorn-like protrusion layer pierce the tissue part to be tested in the plant leaf; S3, tying a thin rope to the semicircular fixing rings on both sides of the upper end of the box body, thereby hanging the box body on a branch or stem near the sampled plant leaves, so that the plant leaves are naturally stretched, and waiting for the juice of the plant leaves to diffuse into the phosphate buffer; S4. After the sampling is completed, the box body is tilted again so that the lower end of the box body is tilted upward, the box cover is opened to take out the plant leaves and then the box cover is closed; the connecting hole is opened by the opening / closing mechanism so that the solution to be tested in the sampling chamber enters the testing chamber, and then the connecting hole is closed by the opening / closing mechanism, and finally the box body is placed horizontally; S5, installing the working electrode, the auxiliary electrode, and the reference electrode on the bracket, then removing the colloid piston on the support at the upper end of the box body, and installing the bracket on the support, so that the working electrode, the auxiliary electrode, and the reference electrode enter the detection chamber of the box body respectively, and contact with the solution to be detected in the detection chamber, and stand still to activate the working electrode; S6. Connect the signal line leading out from the upper end of the bracket to a portable electrochemical workstation to obtain target hormone content data in the plant leaves.

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