Biological seed treatment agents and their application in crop planting

Abstract

The present invention provides a biological seed treatment agent, which comprises: a microbial agent, which is selected from at least one of Bacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound. The biological seed treatment agent of the present invention has the advantages of increasing seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, reducing fertilizer usage, etc., and is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot and alfalfa damping-off, and has good application prospects.

Description

Technical Field

[0001] The present invention relates to the field of agriculture, in particular to a biological seed treatment agent and application thereof in crop planting. Background Art

[0002] Seed treatment is undoubtedly the most cost-effective plant protection method because it can achieve early plant protection, precise pesticide application, labor-saving and labor-saving, and can also manage the seed life cycle and add special attributes to the promotion and sales of new varieties. Among them, seed treatment agents, as one of the main methods of seed treatment, have many advantages such as high efficiency, economy, environmental protection, long-lasting effect, multi-function, and easy mechanized operation. It is an effective way to reduce the use of pesticides and improve the utilization rate of pesticides.

[0003] With the continuous innovation of microbiome technology and the continuous progress of microbial resource library construction, the research, industrialization and application of microorganisms in plant protection and plant nutrition have become possible. The use of microorganisms for seed treatment is another new area with a growing trend, which is in line with the general trend of further developing biological control agents in plant protection. The use of microbial metabolites or living microorganisms as active ingredients is the main research direction of biological seed treatment agents.

[0004] Due to the diversity of soil environments, microbial seed treatment agents have unstable effects. The reason is that the beneficial microorganisms in microbial seed treatment agents proliferate differently in different soil environments. In order to stabilize the use effect of the product, substances that increase the proliferation of beneficial microorganisms in different soil environments need to be added to the product. The metabolites produced during the proliferation of beneficial microorganisms induce crops to produce systemic resistance to adverse environments, thereby improving and stabilizing the application effect of the product. Summary of the invention

[0005] The present invention aims to solve the technical problems existing in the prior art at least to a certain extent. To this end, the present invention proposes a biological seed treatment agent, a method for treating seeds using the biological seed treatment agent, seeds treated with the biological seed treatment agent, the application of the biological seed treatment agent or the seeds treated with the biological seed treatment agent in crop planting, and a crop planting method. The biological seed treatment agent of the present invention has the advantages of increasing seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, reducing fertilizer use, etc., and is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot and alfalfa damping-off, and has good application prospects.

[0006] In one aspect of the present invention, the present invention provides a biological seed treatment agent. According to an embodiment of the present invention, the biological seed treatment agent comprises: a microbial agent, the microbial agent is selected from at least one of Paenibacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound.

[0007] According to the embodiments of the present invention, the inventors have found that the colonization of beneficial microorganisms in the rhizosphere and the soil around the rhizosphere is the key to their function. To this end, the inventors have found through a large number of experiments that the use of Bacillus polymyxa and / or Bacillus amyloliquefaciens to colonize inside or on the surface of seeds, during the growth and development of plants, along the rhizosphere or roots, play a role in nutrition and space competition, improve plant physiological metabolism, induce and improve plant resistance, etc., to achieve the purpose of preventing and controlling pests and diseases, promoting plant growth and development, improving plant resistance and increasing production.

[0008] After further research, the inventors found that by adding iron-containing compounds, the above two microorganisms can be colonized in the soil, especially in the rhizosphere soil of wheat, soybeans and alfalfa, and the seeds treated with microbial agents can be improved in the early growth stage to induce crops to produce systemic resistance to adverse factors through microorganisms and their metabolites. It can not only directly antagonize the pathogens carried by the seeds, but also optimize the crop root-soil-microorganism interaction environment, effectively and specifically enhance the resistance of seeds and seedlings, and play a role in ensuring seed germination, improving crop health, and increasing yield and agricultural product quality. At the same time, it effectively improves fertilizer absorption and utilization, and reduces the use of chemical fertilizers during planting management.

[0009] In another aspect of the present invention, the present invention proposes a method for treating seeds using the aforementioned biological seed treatment agent. According to an embodiment of the present invention, the method includes: mixing the biological seed treatment agent with seeds, and drying to obtain biological seeds. Thus, the biological seeds obtained according to the method of the embodiment of the present invention induce crops to produce systemic resistance to adverse factors through microorganisms and their metabolites in the early stage of growth, enhance stress resistance, improve crop health level, and increase yield and quality of agricultural products. At the same time, it effectively improves fertilizer absorption and utilization rate, reduces the use of chemical fertilizers in the planting management process, and has good application prospects.

[0010] In another aspect of the present invention, the present invention proposes a seed treated with a biological seed treatment agent. According to an embodiment of the present invention, the seed treated with the biological seed treatment agent is obtained by the method of treating seeds with a biological seed treatment agent as described above. Therefore, the seed treated with the biological seed treatment agent according to the embodiment of the present invention induces crops to produce systemic resistance to adverse factors through microorganisms and their metabolites in the early stage of growth, enhances stress resistance, improves crop health, and increases yield and quality of agricultural products. At the same time, it effectively improves fertilizer absorption and utilization rate, reduces the use of chemical fertilizers in the planting management process, and has good application prospects.

[0011] In another aspect of the present invention, the present invention proposes the use of the aforementioned biological seed treatment agent or seeds treated with the biological seed treatment agent in crop planting.

[0012] In another aspect of the present invention, the present invention proposes a method for planting crops. According to an embodiment of the present invention, the method comprises: applying the seeds treated with the biological seed treatment agent according to claim 6 to the soil for cultivation. Therefore, the method according to the embodiment of the present invention can enhance the stress resistance of crops, improve the health level of crops, and increase the yield and quality of agricultural products. At the same time, it effectively improves the absorption and utilization rate of fertilizers, reduces the use of chemical fertilizers in the planting management process, and has good application prospects.

[0013] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. DETAILED DESCRIPTION

[0014] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0015] The present invention provides a biological seed treatment agent, a method for treating seeds using the biological seed treatment agent, seeds treated with the biological seed treatment agent, the application of the biological seed treatment agent or the seeds treated with the biological seed treatment agent in crop planting, and a crop planting method, which will be described in detail below.

[0016] Biological seed treatment agent

[0017] In one aspect of the present invention, the present invention proposes a biological seed treatment agent. According to an embodiment of the present invention, the biological seed treatment agent includes: a microbial agent, the microbial agent is selected from at least one of Bacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound. The biological seed treatment agent of the present invention can improve the seed germination rate, enhance the quality of seedlings, improve stress resistance, prevent and control pests and diseases, increase crop yields, and reduce the amount of fertilizer used. It is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot, and alfalfa damping-off, and has good application prospects.

[0018] According to an embodiment of the present invention, the content of Paenibacillus polymyxa in the biological seed treatment agent is 5 to 30 billion CFU / g or 500 to 300 million CFU / mL (based on the total mass or total volume of the biological seed treatment agent). The inventors obtained the above-mentioned Paenibacillus polymyxa content through a large number of experiments. As a result, under the premise of limited seed surface loading, the effects of improving seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling diseases and insect pests, increasing crop yields, and reducing fertilizer use can be achieved. Among them, 15 to 25 billion CFU / g or 1.5 to 2.5 billion CFU / mL has a better effect, and 20 billion CFU / g or 2 billion CFU / mL is better.

[0019] According to an embodiment of the present invention, the content of Bacillus amyloliquefaciens in the biological seed treatment agent is 20 to 200 billion CFU / g or 20 to 200 billion CFU / mL (based on the total mass or total volume of the biological seed treatment agent). The inventors obtained the above-mentioned Bacillus amyloliquefaciens content through a large number of experiments. As a result, under the premise of limited seed surface loading, the effects of improving seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yields, and reducing fertilizer use can be achieved. Among them, 80 to 120 billion CFU / g or 50 to 120 CFU / mL has a better effect, and 100 billion CFU / g or 10 billion CFU / mL has a better effect.

[0020] According to an embodiment of the present invention, the ratio of the bacterial content of Bacillus polymyxa and Bacillus amyloliquefaciens in the biological seed treatment agent is 1:1 to 1:10. The inventors obtained the above bacterial content and ratio through a large number of experiments, thereby achieving the effects of improving seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, and reducing fertilizer use under the premise of limited seed surface load. Among them, 1:4 to 1:9 has a better effect. For example, the bacterial content in the biological seed treatment agent is 100 billion CFU / g, of which the content of Bacillus polymyxa is 20 billion CFU / g, and the content of Bacillus amyloliquefaciens is 80 billion CFU / g; the bacterial content in the biological seed treatment agent is 10 billion CFU / mL, of which the content of Bacillus polymyxa is 1 billion CFU / mL, and the content of Bacillus amyloliquefaciens is 9 billion CFU / mL.

[0021] According to an embodiment of the present invention, the iron-containing compound is selected from at least one of chelated iron and complexed iron. If free iron ions are used directly, they will be quickly oxidized to high-valent iron, or easily fixed or degraded to insoluble salts when applied to the soil, and are not easily absorbed and utilized by crop growth. Furthermore, the inventor provides iron elements in a stable chelated or complexed state, which can obtain better application effects.

[0022] According to an embodiment of the present invention, the iron-containing compound is selected from one of chitosan oligosaccharide chelated iron, ethylenediaminetetraacetic acid sodium iron (EDTAFeNa), ethylenediamine di-o-hydroxyphenyl sodium iron acetate (EDDHAFeNa), iminodisuccinate iron (IDHAFe) and amino acid chelated iron. Thus, the above iron-containing compound can act on seeds efficiently and stably, and play a better role.

[0023] According to an embodiment of the present invention, based on the total mass of the biological seed treatment agent, the contents of chitosan oligosaccharide chelated iron, sodium ethylenediaminetetraacetate iron, sodium ethylenediamine di-o-hydroxyphenyl acetate iron, iminodisuccinate iron and amino acid chelated iron in the biological seed treatment agent are 0.2-5w / w%, 0.1-2.4w / w%, 0.2-3w / w%, 0.2-3.6w / w% and 0.1-1.5w / w%, respectively. The inventors obtained the above-mentioned preferred dosage through a large number of experiments, thereby enabling the biological seed treatment agent to better play its role in improving seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yields, and reducing fertilizer use.

[0024] According to an embodiment of the present invention, the biological seed treatment agent is a seed treatment dispersible powder, and the seed treatment dispersible powder further includes at least one of the following: sodium carboxymethyl cellulose, lactose, Tween 80 and white carbon. Thus, the wetting and dispersibility can be improved. According to another embodiment of the present invention, in the biological seed treatment agent, based on the total mass of the biological seed treatment agent, the contents of sodium carboxymethyl cellulose, lactose, Tween 80 and white carbon are 0.01-5w / w%, 1-80w / w%, 0.2-3w / w% and 0.01-2w / w%. The inventors obtained the above-mentioned preferred addition amount through a large number of experiments, thereby better increasing the wetting and dispersibility. In some preferred embodiments, the contents of sodium carboxymethyl cellulose, lactose, Tween 80 and white carbon are 0.02-0.04w / w%, 60-80w / w%, 0.2-0.4w / w%, 0.01-0.05w / w%, respectively.

[0025] According to an embodiment of the present invention, the biological seed treatment agent is a suspending agent, and the suspending agent further includes at least one of the following: xanthan gum, polyethylene glycol and sodium benzoate. According to another embodiment of the present invention, in the biological seed treatment agent, based on the total mass of the biological seed treatment agent, the contents of xanthan gum, polyethylene glycol and sodium benzoate are 0.15-0.24w / w%, 0.4-1w / w% and 0.3-0.4w / w%, respectively. The inventors obtained the above-mentioned preferred addition amount through a large number of experiments, thereby better increasing the suspendability, storage stability and safety.

[0026] Method for treating seeds using biological seed treatment agent, seeds treated with biological seed treatment agent, application and crop planting method

[0027] In another aspect of the present invention, the present invention proposes a method for treating seeds using the aforementioned biological seed treatment agent. According to an embodiment of the present invention, the method comprises: mixing the biological seed treatment agent with seeds, and drying to obtain treated seeds. Thus, the seeds obtained by the method according to the embodiment of the present invention induce the crops to produce systemic resistance to adverse factors through microorganisms and their metabolites in the early stage of growth, enhance stress resistance, improve crop health level, and increase yield and quality of agricultural products. At the same time, it effectively improves fertilizer absorption and utilization rate, reduces the use of chemical fertilizers in the planting management process, and has good application prospects.

[0028] According to an embodiment of the present invention, the mass ratio of the biological seed treatment agent to the seeds is 1:30 to 1:80, preferably 1:40 to 1:60, thereby better promoting seed growth.

[0029] It should be noted that, in the method according to the embodiment of the present invention, before mixing the biological seed treatment agent with the seeds, the seed treatment dispersible powder needs to be diluted 5-10 times in advance, and the seed treatment suspension can be used directly without dilution or diluted to a specified volume according to actual seed treatment needs.

[0030] In another aspect of the present invention, the present invention proposes a seed treated with a biological seed treatment agent. According to an embodiment of the present invention, the seed treated with the biological seed treatment agent is obtained by the method of treating seeds with a biological seed treatment agent as described above. Therefore, the seeds according to the embodiment of the present invention induce crops to produce systemic resistance to adverse factors through microorganisms and their metabolites in the early stages of growth, enhance stress resistance, improve crop health, and increase yield and agricultural product quality. At the same time, it effectively improves fertilizer absorption and utilization rate, reduces the use of chemical fertilizers in the planting management process, and has good application prospects.

[0031] In another aspect of the present invention, the present invention proposes the use of the aforementioned biological seed treatment agent or seeds treated with the biological seed treatment agent in crop planting.

[0032] According to an embodiment of the present invention, the crop is selected from wheat, beans, alfalfa, rice, sorghum, and oats.

[0033] In another aspect of the present invention, a method for planting crops is provided. According to an embodiment of the present invention, the method comprises: applying the seeds treated with the above-mentioned biological seed treatment agent to the soil for cultivation.

[0034] The present invention does not strictly limit the cultivation method, and can be processed by a common cultivation method according to the growth characteristics of the crop. According to an embodiment of the present invention, the crop is selected from wheat, beans, alfalfa, rice, sorghum, and oats.

[0035] It should be noted that the characteristics and advantages described above for the biological seed treatment agent are also applicable to the method of treating seeds using the biological seed treatment agent, the seeds treated with the biological seed treatment agent, the application and the crop planting method, and will not be repeated here.

[0036] Beneficial effects:

[0037] The present invention integrates chelated iron or complex iron that is easier to be absorbed by plants with microorganisms to prepare a seed treatment agent, which can not only increase the number of beneficial bacteria colonized in the root system of crops and give full play to the advantages of microorganisms in the management of seed and seedling adversity, but also improve the absorption and utilization of iron by plants, broaden the scope of application, and significantly improve the drought and cold resistance of crops such as wheat, soybeans and alfalfa. The prevention and control efficiency of crops such as wheat sheath blight, soybean root rot and alfalfa damping-off is increased by more than 10% compared with commonly used similar agents, and the yield is increased by more than 20%.

[0038] The scheme of the present invention will be explained below in conjunction with the embodiments. It will be appreciated by those skilled in the art that the following embodiments are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Where specific techniques or conditions are not indicated in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used are not indicated by the manufacturer and are all conventional products that can be obtained commercially.

[0039] In the embodiments of the present invention, the preparation method of the fermentation broth of Bacillus polymyxa and Bacillus amyloliquefaciens is as follows:

[0040] (1) Bacillus amyloliquefaciens KN-527 fermentation broth: Bacillus amyloliquefaciens KN-527 was used, and its deposit number is CCTCC NO: M2015709. Referring to Example 1 of CN101619298B, the Bacillus subtilis strain was replaced with the Bacillus amyloliquefaciens strain to prepare a Bacillus amyloliquefaciens fermentation broth, and the bacterial content after the fermentation culture was completed was 18 to 20 billion CFU / g.

[0041] (2) Paenibacillus polymyxa KN-03 fermentation broth: Paenibacillus polymyxa KN-03 was used, and its deposit number is CCTCC NO: M2012077. Paenibacillus polymyxa fermentation broth was prepared with reference to Example 1 of CN101869181B, and the bacterial content after fermentation culture was 2 to 2.5 billion CFU / g.

[0042] (3) Bacillus amyloliquefaciens KN-527 and Bacillus polymyxa KN-03 mother drugs: obtained by referring to Example 14 and Example 17 in 202111580923.5, respectively, wherein the bacterial content in the obtained Bacillus amyloliquefaciens KN-527 mother drug is 596.5 billion CFU / g, and the spore count in the obtained Bacillus polymyxa KN-03 mother drug is 115.8 billion CFU / g.

[0043] (4) Bacillus subtilis KN-42 fermentation broth: Bacillus subtilis KN-42 was used, and its deposit number is CCTCCNO: M208249. Bacillus subtilis fermentation broth was prepared with reference to Example 1 of CN101619298B, and the bacterial count in the fermentation broth after fermentation culture was completed was 19 to 21 billion CFU / g.

[0044] (5) Bacillus subtilis KN-42 mother drug: obtained by referring to Example 16 of 202111580923.5, the number of Bacillus in the prepared mother drug is 654.8 billion CFU / g.

[0045] Example 1 Preparation method of dispersible powder for seed treatment of Bacillus polymyxa

[0046] The components and contents of the seed treatment dispersible powder are as follows: about 20% (w / w) of the parent drug of Paenibacillus polymyxa KN-03 (after testing, the final content of viable bacteria is 21 billion CFU / g), 0.6% (w / w) of chitosan oligosaccharide chelated iron, 0.01% (w / w) of sodium carboxymethyl cellulose, 0.2% (w / w) of Tween 80, 0.01% (w / w) of white carbon black, and lactose is supplemented to 100%, and the mixture is mixed. It is named seed treatment agent No. 1.

[0047] Example 2 Preparation method of polymyxa bacillus seed treatment suspension

[0048] The components and contents of the seed treatment suspension are as follows: 0.22% (w / w) of ethylenediaminetetraacetic acid sodium iron (EDTAFeNa), 0.15% (w / w) of xanthan gum, 0.4% (w / w) of polyethylene glycol, and 0.3% of sodium benzoate are added to the mother solution of Paenibacillus polymyxa KN-03, and mixed well. After testing, the final content of live bacteria is 2.1 billion CFU / mL. It is named seed treatment agent No. 2.

[0049] Example 3 Preparation method of dispersible powder for treating Bacillus amyloliquefaciens seeds

[0050] The components and contents of the seed treatment dispersible powder are as follows: 25% (w / w) of Bacillus amyloliquefaciens KN-527 mother drug (tested, the final content of live bacteria is 108 billion CFU / g), 1.2% (w / w) of ethylenediamine di-o-hydroxyphenyl sodium ferric acetate (EDDHAFeNa), 0.03% (w / w) of sodium carboxymethyl cellulose, 0.25% (w / w) of Tween 80, 0.03% (w / w) of white carbon black, and lactose is supplemented to 100%, and the mixture is mixed. The mixture is named seed treatment agent No. 3.

[0051] Example 4 Preparation method of Bacillus amyloliquefaciens seed treatment suspension

[0052] The components and contents of the seed treatment suspension are as follows: 0.38% (w / w) of iminodisuccinate iron (IDHAFe), 0.2% (w / w) of xanthan gum, 0.4% (w / w) of polyethylene glycol, and 0.32% of sodium benzoate are added to the mother solution of Bacillus amyloliquefaciens KN-527, and mixed well. After testing, the final content of the viable bacteria count is 11 billion CFU / mL. It is named seed treatment agent No. 4.

[0053] Example 5 Preparation method of composite seed treatment dispersible powder

[0054] The components and contents of the seed treatment dispersible powder are as follows: 20% (w / w) of polymyxa bacillus KN-03 mother drug, 15% (w / w) of bacillus amyloliquefaciens KN-03 mother drug (the total final bacterial count of the two is about 100 billion CFU / g), 0.6% (w / w) of chitosan oligosaccharide chelated iron, 0.02% (w / w) of sodium carboxymethyl cellulose, 0.2% (w / w) of Tween 80, 0.01% (w / w) of white carbon black, and lactose is supplemented to 100%, and mixed well. After testing, the final content of viable bacterial count of polymyxa bacillus KN-03 is 19.5 billion CFU / g, and that of bacillus amyloliquefaciens KN-527 is 81 billion CFU / g. It is named seed treatment agent No. 5.

[0055] Example 6 Preparation method of composite seed treatment suspension

[0056] The components and contents of the seed treatment suspension are as follows: the mother liquor of Paenibacillus polymyxa KN-03 and the mother liquor of Bacillus amyloliquefaciens KN-527 are mixed at a ratio of 4:5 (w / w), and 0.20% (w / w) of amino acid chelated iron, 0.2% (w / w) of xanthan gum, 0.4% (w / w) of polyethylene glycol, and 0.32% of sodium benzoate are added to the mixed fermentation liquid, and the mixture is mixed. After testing, the final content of viable bacteria count of Paenibacillus polymyxa KN-03 is 1.1 billion CFU / mL, and that of Bacillus amyloliquefaciens KN-527 is 9.2 billion CFU / mL. It is named Seed Treatment Agent No. 6.

[0057] Comparative Example 1 Preparation method of dispersible powder for seed treatment of Bacillus subtilis KN-42

[0058] The components and contents of the seed treatment dispersible powder are as follows: 17% (w / w) of Bacillus subtilis mother drug (the final content of live bacteria is about 105 billion CFU / g), 0.6% (w / w) of chitosan oligosaccharide chelated iron, 0.02% (w / w) of sodium carboxymethyl cellulose, 0.2% (w / w) of Tween 80, 0.01% (w / w) of white carbon black, and lactose is supplemented to 100%, and the mixture is mixed. The mixture is named seed treatment agent No. 7.

[0059] Comparative Example 2 Preparation method of dispersible powder for seed treatment of Bacillus subtilis KN-42

[0060] The components and contents of the seed treatment suspension are as follows: adding 0.38% (w / w) IDHAFe, 0.2% (w / w) xanthan gum, 0.4% (w / w) polyethylene glycol, and 0.32% sodium benzoate to the Bacillus subtilis KN-mother liquor, and mixing well. After testing, the final content of viable bacteria is 11 billion CFU / mL. It is named seed treatment agent No. 8.

[0061] Comparative Example 3

[0062] Seed treatment agents were prepared according to the preparation methods of Examples 1 to 6 and Comparative Example 1, except that none of them contained iron-containing compounds. The prepared seed treatment agents were named Seed Treatment Agents No. 9-15.

[0063] Example 7

[0064] The seed treatment agents of Examples 1 to 6 and Comparative Examples 1 to 3 were diluted with an appropriate amount of water according to the dosage in Table 1 to obtain a liquid medicine. Then, the seeds of each crop were treated with the liquid medicine. Specifically, the mass ratio of the liquid medicine to the seeds of wheat, soybeans and alfalfa was 1:50 (for example, 100 kg of seeds were evenly mixed with 2 kg of liquid medicine). In the conventional control group, the agent used in the wheat group was 60 g / L tebuconazole seed treatment suspension, and the dosage was 50 g / 100 kg of seeds; the agent used in the soybean group was 25 g / L fludioxonil suspension seed coating agent, and the dosage was 600 g / 100 kg of seeds; the agent used in the alfalfa group was 1 billion spores / mL rhizobium suspension, and the dosage was 2 kg / 100 kg of seeds.

[0065] Table 1 Usage and dosage of various seed treatment agents

[0066]

[0067]

[0068] After drying, the following experiments were performed:

[0069] 1. The treated wheat, soybean and alfalfa seeds were subjected to field trials respectively. The trials were selected in areas or plots where the diseases occurred in previous years. The cultivation conditions (soil type, fertilization, sowing rate, sowing period, growth stage, plant spacing, etc.) and management of the test plots were consistent and in line with the local good agricultural practice (GAP). Each treatment was repeated 4 times, and the area of ​​each plot was 30 square meters.

[0070] Table 2 Application effect of seed treatment agents on wheat

[0071]

[0072]

[0073] Table 3 Application effect of seed treatment agents on soybean

[0074]

[0075] Table 4 Application effect of seed treatment agents on alfalfa

[0076]

[0077]

[0078] As can be seen from Tables 2 to 4, the germination rate and seedling rate of wheat, soybean and alfalfa seeds treated with seed treatment agents 1 to 6 were significantly higher than those of the conventional control group and the clear water control group, indicating that the treatment of wheat, soybean and alfalfa seeds with seed treatment agents 1 to 6 is safe; the growth rate of above-ground fresh weight of crops, the growth rate of underground fresh weight, the growth rate of disease prevention effect and the yield increase rate of wheat, soybean and alfalfa seeds treated with seed treatment agents 1 to 6 were significantly higher than those of seed treatment agents 9-15, the conventional control group and the clear water control group, indicating that seed treatment agents 1 to 6 can effectively promote the crops to play the role of strong seedlings, disease prevention and yield increase.

[0079] Seed treatment agent No. 7 (Comparative Example 1) and seed treatment agent No. 8 (Comparative Example 2) use Bacillus subtilis KN-42 to treat seeds. Bacillus subtilis is a commonly used seed treatment agent in the art, but the germination rate, seedling rate, aboveground fresh weight growth, underground fresh weight growth, sheath blight control rate and yield increase rate after treatment are not as good as those in Examples 1-6, indicating that the application effect of the product can not be improved by adding chelated iron or complexed iron to the seed treatment using Bacillus.

[0080] Seed treatment agent No. 7 used Bacillus subtilis and iron-containing compounds to treat seeds together, while seed treatment agent No. 15 used Bacillus subtilis and did not use iron-containing compounds to treat seeds. After treatment with seed treatment agent No. 7, the germination rate, seedling rate, above-ground fresh weight growth, underground fresh weight growth, sheath blight control rate and yield increase rate were all lower than those of seed treatment agent No. 15. This shows that the combined action of iron-containing compounds and bacterial agents did not achieve the expected synergistic effect. On the contrary, the addition of iron-containing compounds hindered the effect of Bacillus subtilis on crops.

[0081] After the crops were treated with seed treatment agents 1 to 6, the germination rate, seedling rate, aboveground fresh weight growth, underground fresh weight growth, sheath blight control rate and yield increase rate were all higher than those of the corresponding treatment agents 9-14 without iron-containing compounds, indicating that iron-containing compounds can effectively promote the growth of Bacillus polymyxa KN-03 and Bacillus amyloliquefaciens KN-527, and play a role in strengthening seedlings, preventing diseases and increasing yields.

[0082] 2. The treated seeds were sown in the field, sieved (60 mesh) after air drying, baked in a drying oven for 6 hours, and sown in the soil after cooling. The seeds were cultured under drought (soil relative moisture content was 12%) and low temperature (15℃ for 16 hours, 8℃ for 8 hours) conditions, and the germination rate, seedling rate and fresh weight of wheat, soybean and alfalfa seeds in each treatment group were determined.

[0083] The results are shown below. It can be seen from Tables 5 to 6 that the germination rate and seedling rate of seeds treated with seed treatments No. 1 to 6 under drought (relative soil moisture content of 12%) and low temperature conditions (15°C, 16h, 8°C, 8h) were significantly improved compared with the conventional control group, the clear water control group, the seed treatment groups No. 7 to 8 and the seed treatment groups No. 9 to 15. The fresh weight of the aboveground and underground parts increased by more than 20%, and the overall quality of the seedlings was significantly higher than that of the seed treatments No. 7 to 15, the treatment group control group, the conventional control group and the clear water control group. This mainly shows that the use effect of Bacillus polymyxa KN-03 or / and Bacillus amyloliquefaciens KN-527 is better than that of Bacillus subtilis KN-42, and iron-containing compounds can effectively promote Bacillus polymyxa KN-03 or / and Bacillus amyloliquefaciens KN-527 to improve the germination rate and seedling rate of seeds under low temperature and drought adversity.

[0084] Table 5 Effects of seed treatment agents on wheat, soybean and alfalfa under drought

[0085]

[0086]

[0087] Table 6 Effects of seed treatment agents on wheat, soybean and alfalfa under low temperature

[0088]

[0089]

[0090] 3. Seeds treated with seed treatment agents 1 to 15 and conventional control agents were sown in field soil with 15% less basal fertilizer, and other management and drug application conditions were kept the same. The final yields of wheat, soybeans and alfalfa were measured.

[0091] The results are shown in Table 7. Under the condition of 15% weight loss, after the wheat, soybean and alfalfa seeds were treated with the seed treatment agents No. 1-6 provided by the present invention, the final yield increase of wheat, soybean and alfalfa can still reach 10-16%, which is significantly higher than that of the conventional control group, the seed treatment agent No. 7-8 treatment group and the seed treatment agent No. 9-15 treatment group, indicating that the use effect of Bacillus polymyxa KN-03 and / or Bacillus amyloliquefaciens KN-527 is better than that of Bacillus subtilis KN-42, and the iron-containing compound can effectively promote Bacillus polymyxa KN-03 and / or Bacillus amyloliquefaciens KN-527 to improve the absorption and utilization rate of fertilizers by crops.

[0092] Table 7 Yield-increasing effect of seed treatment agents on wheat, soybean and alfalfa when weight loss was 15%

[0093]

[0094]

[0095] 4. Determine the colonization effect of Paenibacillus polymyxa KN-03 or / and Bacillus amyloliquefaciens KN-527 on the rhizosphere of barley, soybean and alfalfa seeds after seed treatment agents 1 to 6 (with added chitosan oligosaccharide chelated iron) were treated with seed treatment agents 9 to 15 (without added chitosan oligosaccharide chelated iron). During the field test of Example 7-1, 30 days after sowing wheat, soybean and alfalfa seeds, rhizosphere soil samples of the crops were taken, and the number of Paenibacillus polymyxa KN-03 or / and Bacillus amyloliquefaciens KN-527 in the rhizosphere soil was determined by the dilution coating method.

[0096] The results are shown in Table 8. After adding chitosan oligosaccharide chelated iron to the seed treatment agent, the colonization number of Bacillus polymyxa KN-03 and / or Bacillus amyloliquefaciens KN-527 in the rhizosphere soil of wheat, soybean and alfalfa increased by more than 50% compared with the seed treatment agent without adding chelated iron.

[0097] Table 8 Colonization effect of seed treatment formulations without iron on the rhizosphere of wheat, soybean and alfalfa

[0098]

[0099] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A biological seed treatment agent, characterized in that: include: A microbial agent, wherein the microbial agent is selected from at least one of Paenibacillus polymyxa and Bacillus amyloliquefaciens; Iron compounds; The deposit number of the polymyxa bacillus is CCTCC NO: M2012077, and the depository is China Center for Type Culture Collection; The deposit number of the Bacillus amyloliquefaciens is CCTCC NO: M2015709, and the depository unit is China Center for Type Culture Collection.

2. The biological seed treatment agent according to claim 1, characterized in that The content of Paenibacillus polymyxa in the biological seed treatment agent is 5 to 30 billion CFU / g or 5 to 3 billion CFU / mL; The content of Bacillus amyloliquefaciens in the biological seed treatment agent is 20 to 200 billion CFU / g or 2 to 20 billion CFU / mL; The ratio of the bacterial content of Bacillus polymyxa to that of Bacillus amyloliquefaciens in the biological seed treatment agent is 1:1-1:

10.

3. The biological seed treatment agent according to claim 1, characterized in that The iron-containing compound is selected from chelated iron and complexed iron.

4. The biological seed treatment agent according to claim 1, characterized in that The iron-containing compound is selected from one of chitosan oligosaccharide chelated iron, sodium ethylenediaminetetraacetate iron, sodium ethylenediamine di-o-hydroxyphenyl acetate iron, iminodisuccinate iron and amino acid chelated iron.

5. The biological seed treatment agent according to claim 4, characterized in that Based on the total mass of the biological seed treatment agent, the contents of chitosan oligosaccharide chelated iron, sodium ethylenediaminetetraacetate iron, sodium ethylenediamine di-o-hydroxyphenyl acetate iron, iron iminodisuccinate and amino acid chelated iron in the biological seed treatment agent are 0.2-5w / w%, 0.1-2.4w / w%, 0.2-3w / w%, 0.2-3.6w / w% and 0.1-1.5w / w%, respectively.

6. The biological seed treatment agent according to claim 1, characterized in that The biological seed treatment agent is a seed treatment dispersible powder, and the seed treatment dispersible powder further comprises at least one of the following: sodium carboxymethyl cellulose, lactose, Tween 80 and white carbon black.

7. The biological seed treatment agent according to claim 6, characterized in that In the biological seed treatment agent, based on the total mass of the biological seed treatment agent, the contents of sodium carboxymethyl cellulose, lactose, Tween 80 and white carbon are 0.01-5w / w%, 1-80w / w%, 0.2-3w / w% and 0.01-2w / w% respectively.

8. The biological seed treatment agent according to claim 1, characterized in that The biological seed treatment agent is a suspending agent, and the suspending agent further comprises at least one of the following: xanthan gum, polyethylene glycol and sodium benzoate.

9. The biological seed treatment agent according to claim 8, characterized in that In the biological seed treatment agent, based on the total mass of the biological seed treatment agent, the contents of xanthan gum, polyethylene glycol and sodium benzoate are 0.15-0.24 w / w%, 0.4-1 w / w% and 0.3-0.4 w / w%, respectively.

10. A method for treating seeds using the biological seed treatment agent according to any one of claims 1 to 9, characterized in that: include: The biological seed treatment agent is mixed with seeds and dried to obtain treated seeds.

11. The method according to claim 10, characterized in that The mass ratio of the biological seed treatment agent to the seeds is 1:30 to 1:

80.

12. Use of the biological seed treatment agent according to any one of claims 1 to 9 or the seeds treated with the biological seed treatment agent according to claim 10 or 11 in crop planting.

13. The use according to claim 12, characterized in that: The crops are selected from wheat, beans, alfalfa, rice, sorghum and oats.

14. A method for planting crops, characterized in that: include: The seeds treated by the method for treating seeds with a biological seed treatment agent according to claim 10 or 11 are applied to the soil for cultivation.

15. The method according to claim 14, characterized in that The crops are selected from wheat, beans, alfalfa, rice, sorghum and oats.

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