Experimental device and method for simulating the effect of dust explosion on bacterial colony growth in confined environment

Abstract

The present invention discloses an experimental device for simulating the effect of dust explosion on the growth of bacterial colonies in a confined environment, comprising an air supply module, a cavity module, a water supply module, a temperature control module, an ignition module and a data acquisition module connected to each other; a mixed spraying of various dusts, ignition and explosion, as well as protection and growth monitoring of bacterial colonies in water are achieved in the spherical explosion zone. An experimental method for the above-mentioned device is also provided. The beneficial effects of the present invention are that the structure of the experimental device is reasonably designed, and the explosion reaction material is prevented from leaking, which comprehensively affects the growth process of the bacterial colony; the visual window and the bacterial colony sampling port can observe the growth of the bacterial colony, as well as the growth status before and after the explosion reaction; by adjusting the amount of dust, type of dust, amount of water, type and number of bacterial colonies, etc., the effects of dust explosion and reaction products on the growth of various bacterial colonies under multi-factor conditions are studied, and the experimental method is simple to operate, saves time, and is convenient and fast.

Description

Technical Field

[0001] The present invention relates to the technical field of dust explosion experiments, and in particular to an experimental device and method for simulating the influence of dust explosion on bacterial colony growth in a confined environment. Background Art

[0002] Dust explosions can produce a large number of reaction products, including gaseous products and solid products. These reaction products may have a significant impact on the ecological environment. Therefore, studying the impact mechanism of dust explosions on bacterial growth in confined environments is a technical and theoretical support for environmental protection.

[0003] Typically, experiments investigating dust explosions and their environmental impacts require two experimental scenarios: Scenario 1 involves collecting reaction products after the dust explosion; Scenario 2 involves adding reaction products to a bacterial colony culture environment and observing bacterial growth. This experimental method, due to omission of on-site modular reaction products, can lead to significant errors in experimental results. The unavoidable impact of the explosion on bacterial colonies during the explosion itself is a key reason for the use of multiple experimental scenarios in this type of research.

[0004] Therefore, it is necessary to develop an experimental device that simulates the impact of dust explosions on colony growth in a confined environment, and to propose corresponding experimental methods to achieve dust explosions and colony cultivation in the same space within the same scenario, correct experimental errors, and facilitate environmental experiments. This is a technical problem that technical personnel in this field currently need to solve. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention discloses an experimental device and method for simulating the effect of dust explosion on bacterial colony growth in a confined environment.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] An experimental device for simulating the effect of dust explosion on bacterial colony growth in a confined environment, comprising an air supply module, a cavity module, a water supply module, a temperature control module, an ignition module, and a data acquisition module connected to each other;

[0008] The air supply module includes an air compressor, an air storage chamber, a nozzle, and a vacuum pump connected by pipelines. The air compressor generates compressed air and flows into the air storage chamber. When the pressure reaches a certain level, the solenoid valve opens, and the gas is sprayed into the cavity through the nozzle. The vacuum pump extracts part of the air from the cavity to balance the air pressure inside and outside the cavity.

[0009] The cavity module includes a base and a sphere supported by the base. The sphere includes a spherical tank cover and a cavity. The cavity is divided into an upper explosion zone and a lower water storage zone by a hard explosion relief plate. The explosion zone includes two dust pans and two nozzles, each dust pan corresponds to one nozzle. The water storage zone is used to store bacterial colonies and nutrient solution. After air is supplied and blown, dust is diffused inside the cavity and ignited by the ignition module to achieve a dust explosion. The cavity environment after the explosion serves as the growth environment of the bacterial colonies in the water storage zone.

[0010] The water supply module includes a water pump and a water inlet pipe connected by pipelines, and water is supplied to the water storage area of ​​the cavity module through the water pump and the water inlet pipe;

[0011] The temperature control module includes a temperature control layer formed by a sphere and an insulation layer wrapped around the outer surface of the sphere, as well as a temperature controller and a heating wire. The temperature controller controls the heating wire to heat and control the deionized water in the temperature control layer, so that the spatial temperature of the cavity module can be controlled;

[0012] The ignition module includes an ignition energy generator and two ignition electrodes connected to each other;

[0013] The data acquisition module includes a main controller, a temperature and pressure signal collector, a temperature controller, a water supply and gas supply controller, a pressure sensor, a temperature sensor and a camera. The temperature and pressure signal collector transmits the signals collected from the pressure sensor and the temperature sensor to the main controller, and the main controller also receives signals transmitted from the temperature controller, the water supply and gas supply controller.

[0014] Optionally, the cavity is connected to a water supply module, the water is located in a water storage area, and a filter membrane is provided in the water storage area to achieve separation between water and bacterial colonies.

[0015] Optionally, the water storage area is connected to a liquid level meter for observing water level changes.

[0016] Optionally, a sampling port is provided outside the cavity, and the position where the sampling port is connected to the cavity is located above the filter membrane.

[0017] Optionally, the spherical tank cover includes a handle and a flange, and the spherical tank cover is connected to the cavity via bolts on the flange.

[0018] Optionally, the ignition electrode passes through the spherical tank cover. After the spherical tank cover is installed on the cavity, the two ignition electrodes are located in the middle of the cavity, and the distance between the two ignition electrodes is no more than 6 mm.

[0019] Optionally, a visual window is provided on the cavity, and the visual window is located at the water storage area. The liquid level of the water storage area is located at the horizontal center of the visual window, and the shooting machine captures the picture through the visual window.

[0020] The present invention also provides an experimental method using the above device, comprising the following steps:

[0021] (1) Assemble the experimental apparatus;

[0022] (2) Weigh the dust and place it in a dust pan;

[0023] (3) Turn on the thermostat and adjust the temperature;

[0024] (4) Turn on the water pump to supply water to the water storage area, and add bacterial colonies and nutrient solution into the water;

[0025] (5) Install the hard explosion venting disc;

[0026] (6) Cover the spherical tank cover and tighten it with bolts;

[0027] (7) Turn on the vacuum pump to extract air;

[0028] (8) Turn on the air compressor to supply air to the air storage chamber. When the pressure is reached, turn off the air compressor;

[0029] (9) Setting the ignition energy value and ignition delay time of the ignition energy generator;

[0030] (10) The main controller opens the solenoid valve, and the air in the air storage chamber lifts the dust through the nozzle to form a dust cloud;

[0031] (11) After the ignition delay time, the ignition electrode discharges and ignites the dust cloud. After the dust is successfully ignited, the shock wave breaks the hard explosion venting membrane, and the air and reactants after the dust explosion fill the cavity module;

[0032] (12) The colonies in the water storage area survive in the post-explosion environment. The colony production is observed through the visual window and the filming machine is used to photograph the colony, and the colonies are sampled and observed through the sampling port.

[0033] The beneficial effect of the present invention is that the experimental device of the present invention is provided with an air supply module, a cavity module, a water supply module, a temperature control module, an ignition module and a data acquisition module which are connected to each other, wherein the hard explosion-proof plate separates the upper explosion zone and the lower water storage area, which can prevent dust from being blown up and falling into the water, resulting in a reduction in the content of dust participating in the explosion; the colonies and nutrient solution are placed in the lower water storage area, which can effectively avoid the influence of the flame wave and shock wave generated by the explosion on the colonies; the filter membrane can separate the colonies from the aqueous solution, which is convenient for sampling colony samples; the visual window is combined with the shooting machine to better record the growth of the colonies in the confined space, as well as the growth status before and after the explosion reaction; in addition, by adjusting the dust amount, dust type, air temperature, colony type, etc., the influence mechanism of dust explosion on the growth of colonies in a confined environment under multi-factor conditions is studied; the structural design of the experimental device is reasonable, which realizes that the explosion reaction material does not leak and comprehensively affects the growth process of the colonies; the experimental method is simple to operate, which is conducive to saving the time of scientific researchers and is convenient and fast. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a schematic diagram of the structure of the experimental device for simulating the effect of dust explosion on bacterial colony growth in a confined environment according to the present invention;

[0035] Among them, 1. Main controller; 2. Ignition energy generator; 3. Temperature and pressure signal collector; 4. Temperature controller; 5. Ball tank cover; 6. Bolts; 7. Pressure sensor; 8. Temperature sensor; 9. Liquid level gauge; 10. Base; 11. Dust tray I; 12. Nozzle I; 13. Water storage area; 14. Filter membrane; 15. Water outlet pipe; 16. Water outlet pipe valve; 17. Handle; 18. Ignition electrode; 19. Flange; 20. Dust tray II ; 21. Air outlet pipe; 22. Nozzle II; 23. Visual window; 24. Water inlet pipe; 25. Water inlet pipe valve; 26. Sphere; 27. Insulation layer; 28. Solenoid valve; 29. ​​Vacuum pump; 30. Air storage chamber pressure gauge; 31. Air storage chamber; 32. Air storage chamber valve; 33. Water and air supply controller; 34. Air compressor; 35. Camera; 36. Water pump; 37. Hard explosion venting disc; 38. Sampling port; 39. Heating wire. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiment is only a module embodiment of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] An experimental device that simulates the effect of dust explosion on bacterial growth in a confined environment, such as Figure 1As shown, it includes a connected air supply module, a cavity module, a water supply module, a temperature control module, an ignition module and a data acquisition module;

[0038] The air supply module includes an air compressor 34, an air storage chamber 31, a nozzle I12, a nozzle II22, and a vacuum pump 29 connected by pipelines. The air compressor 34 generates compressed air, which is then fed into the air storage chamber 31. An air storage chamber valve 32 is installed on the pipeline between the air compressor 34 and the air storage chamber 31. An air storage chamber pressure gauge 30 is installed on the air storage chamber 31. When the pressure is reached, the solenoid valve 28 opens, and the gas is sprayed into the cavity through the nozzle I12 and the nozzle II 22. The vacuum pump 29 extracts some air from the cavity to balance the air pressure inside and outside the cavity.

[0039] The cavity module includes a base 10 and a sphere 26 supported by the base 10. The sphere 26 includes a spherical tank cover 5 and a cavity. The cavity is divided into an upper explosion zone and a lower water storage zone 13 by a hard explosion relief plate 37. The explosion zone includes a dust pan I11, a dust pan II 20, and nozzles I12 and II 22. Each dust pan corresponds to a nozzle. The water storage zone 13 is used to place bacterial colonies and nutrient solution. After air is blown, dust is diffused inside the cavity and ignited by the ignition module to achieve a dust explosion. The cavity environment after the explosion serves as the growth environment of the bacterial colony in the water storage zone 13.

[0040] The water supply module includes a water pump 36 and a water inlet pipe 24 connected by pipelines. The water inlet pipe 24 is equipped with a water inlet valve 25. Water is supplied to the water storage area 13 of the cavity module through the water pump 36 and the water inlet pipe 24.

[0041] The temperature control module includes a temperature control layer formed by a sphere 26 and an insulation layer 27 wrapped around the outer surface of the sphere 26, as well as a temperature controller 4 and a heating wire 39. The temperature controller 4 controls the heating wire 39 to heat and control the deionized water in the temperature control layer, so that the spatial temperature of the cavity module can be controlled;

[0042] The ignition module includes an ignition energy generator 2 and two ignition electrodes 18 connected to each other;

[0043] The data acquisition module includes a main controller 1, a temperature and pressure signal collector 3, a temperature controller 4, a water supply and gas supply controller 33, a pressure sensor 7, a temperature sensor 8 and a shooting machine 35. The temperature and pressure signal collector 3 transmits the signals collected from the pressure sensor 7 and the temperature sensor 8 to the main controller 1, and the main controller 1 also receives signals transmitted by the temperature controller 4 and the water supply and gas supply controller 33.

[0044] Optionally, the cavity is connected to a water supply module, and water is located in a water storage area 13 . The water storage area 13 is provided with a filter membrane 14 for achieving separation between water and bacterial colonies.

[0045] Optionally, the water storage area 13 is connected to a liquid level meter 9 for observing water level changes.

[0046] Optionally, a sampling port 38 is provided outside the cavity, and the position where the sampling port 38 is connected to the cavity is located above the filter membrane 14 .

[0047] Optionally, the spherical tank cover 5 includes a handle 17 and a flange 19 , and the spherical tank cover 5 is connected to the cavity via bolts 6 on the flange 19 .

[0048] Optionally, the ignition electrode 18 passes through the spherical tank cover 5. After the spherical tank cover 5 is installed on the cavity, the two ignition electrodes 18 are located in the middle of the cavity, and the distance between the two ignition electrodes 18 is no more than 6 mm.

[0049] Optionally, a visual window 23 is provided on the cavity, and the visual window 23 is located at the water storage area 13. The liquid level of the water storage area 13 is located at the horizontal center of the visual window 23, and the shooting machine 35 captures the picture through the visual window.

[0050] Optionally, a water outlet pipe 15 is further provided at the bottom of the sphere 26 , and a water outlet pipe valve 16 is installed on the water outlet pipe 15 .

[0051] Optionally, an air outlet pipe 21 is further provided on one side of the sphere 26 , and the air outlet pipe 21 is connected to a vacuum pump 29 .

[0052] An experimental method for studying the effect of dust explosions on bacterial colony growth in a confined environment, using the above-mentioned experimental apparatus, comprises the following steps:

[0053] (1) Assemble the experimental apparatus;

[0054] (2) Select the type of dust, such as grain dust, mineral dust, polymer dust, metal dust, etc., weigh the dust in the range of 1 to 100 g, and place the weighed dust in the dust tray I11 and the dust tray II 20;

[0055] (3) Turn on the thermostat 4 and adjust the temperature to control the temperature range of 30 to 80°C;

[0056] (4) Turn on the water pump 36 to supply water to the water storage area 13, and add the bacterial colony and nutrient solution into the water;

[0057] (5) Install the hard explosion relief plate 37;

[0058] (6) Cover the spherical tank cover 5 and tighten it with bolts 6;

[0059] (7) Turn on the vacuum pump 29 and extract a certain amount of air, the amount of air extracted ranges from 2 to 3 L;

[0060] (8) Turn on the air compressor 34 to supply air to the air storage chamber 31. After the pressure is reached, the amount of air injected is in the range of 2 to 3 L, and then turn off the air compressor 34;

[0061] (9) Setting the ignition energy value and ignition delay time of the ignition energy generator 2. The ignition energy value setting range is 1mJ to 10J;

[0062] (10) The main controller 1 controls the solenoid valve 28 to open, and the air in the air storage chamber 31 passes through the nozzles I12 and II 22 to lift the dust, forming a dust cloud;

[0063] (11) After the ignition delay time, the ignition electrode 18 discharges and ignites the dust cloud. After the dust is successfully ignited, the shock wave breaks the hard explosion venting plate 37, and the air and reactants after the dust explosion fill the cavity module;

[0064] (12) The bacterial colonies in the water storage area 13 survive in the post-explosion environment. The bacterial colony production is observed through the visual window 23 and the photographing machine 35 photographs the bacterial colony production, and the bacterial colonies are sampled and observed through the sampling port 38.

[0065] Example 1

[0066] Test the colony growth under different concentrations of different types of dust

[0067] (1) Assemble the experimental apparatus;

[0068] (2) Flour, coal powder, and aluminum powder were selected, and dust with a mass of 10g, 20g, 30g, and 40g was weighed respectively. The experiment tested flour, coal powder, and aluminum powder in order. Each test dust was tested in order from low to high mass, as shown in Table 1. The dust of each test was placed in dust pan I11 and dust pan II 20;

[0069] Table 1 Various types of dust test items

[0070]

[0071] (3) Turn on the temperature controller 4 and adjust the temperature to 60°C;

[0072] (4) Turn on the water pump 36 to supply water to the water storage area 13, and add the yeast colony and nutrient solution into the water;

[0073] (5) Install the hard explosion relief plate 37;

[0074] (6) Cover the spherical tank cover 5 and tighten it with bolts 6;

[0075] (7) Turn on the vacuum pump 29 and extract 2L of air;

[0076] (8) Turn on the air compressor 34 to supply air to the air storage chamber 31. After the pressure is reached, inject 2L of air and turn off the air compressor 34.

[0077] (9) Set the ignition energy value and ignition delay time of ignition energy generator 2. The ignition energy value setting range is 2J, and the ignition delay time is 60ms for flour, 60ms for coal powder, and 30ms for aluminum powder.

[0078] (10) The main controller 1 controls the solenoid valve 28 to open, and the air in the air storage chamber 31 passes through the nozzles I12 and II 22 to lift the dust, forming a dust cloud;

[0079] (11) After the ignition delay time, the ignition electrode 18 discharges and ignites the dust cloud. After the dust is successfully ignited, the shock wave breaks the hard explosion venting plate 37, and the air and reactants after the dust explosion fill the cavity module;

[0080] (12) The bacterial colonies in the water storage area 13 survive in the post-explosion environment. The bacterial colony production is observed through the visual window 23 and the photographing machine 35 photographs the bacterial colony production, and the bacterial colonies are sampled and observed through the sampling port 38.

[0081] Example 2

[0082] Test the colony growth under different environmental temperature conditions

[0083] (1) Assemble the experimental apparatus;

[0084] (2) Select aluminum powder, weigh 10 g of dust, and place the dust for each test in dust pan I11 and dust pan II 20;

[0085] (3) Turn on the temperature controller 4 and adjust the temperature to 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, and 80°C, respectively. Set a temperature value for each experiment in ascending order.

[0086] (4) Turn on the water pump 36 to supply water to the water storage area 13, and add the yeast colony and nutrient solution into the water;

[0087] (5) Install the hard explosion relief plate 37;

[0088] (6) Cover the spherical tank cover 5 and tighten it with bolts 6;

[0089] (7) Turn on the vacuum pump 29 and extract 2L of air;

[0090] (8) Turn on the air compressor 34 to supply air to the air storage chamber 31. After the pressure is reached, inject 2L of air and turn off the air compressor 34.

[0091] (9) Setting the ignition energy value and ignition delay time of the ignition energy generator 2. The ignition energy value setting range is 2J, and the ignition delay time is 30ms;

[0092] (10) The main controller 1 controls the solenoid valve 28 to open, and the air in the air storage chamber 31 passes through the nozzles I12 and II 22 to lift the dust, forming a dust cloud;

[0093] (11) After the ignition delay time, the ignition electrode 18 discharges and ignites the dust cloud. After the dust is successfully ignited, the shock wave breaks the hard explosion venting plate 37, and the air and reactants after the dust explosion fill the cavity module;

[0094] (12) The bacterial colonies in the water storage area 13 survive in the post-explosion environment. The bacterial colony production is observed through the visual window 23 and the photographing machine 35 photographs the bacterial colony production, and the bacterial colonies are sampled and observed through the sampling port 38.

[0095] The present invention can simulate the actual process, such as adjusting the dust amount, dust type, air temperature, bacterial colony type, etc., to study the impact mechanism of dust explosion on bacterial colony growth in a confined environment under multi-factor conditions.

[0096] The experimental device has a reasonable structural design and the experimental method is simple to operate, which is beneficial to saving scientific researchers' time and is convenient and quick.

[0097] Furthermore, compared to existing technologies, this experimental device allows for dust explosions and bacterial colony cultivation to occur in the same space, ensuring that the explosion reaction products fully participate in the environmental experiment and improving experimental accuracy. Furthermore, the hard rupture disc 37 and the water environment of the water storage area 13 provide protection for the bacterial colony in an explosive environment.

[0098] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. An experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment, characterized in that: It includes a connected air supply module, a cavity module, a water supply module, a temperature control module, an ignition module and a data acquisition module; The air supply module includes an air compressor, an air storage chamber, a nozzle, and a vacuum pump connected by pipelines. The air compressor generates compressed air and flows into the air storage chamber. When the pressure reaches a certain level, the solenoid valve opens, and the gas is sprayed into the cavity through the nozzle. The vacuum pump extracts part of the air from the cavity to balance the air pressure inside and outside the cavity. The cavity module includes a base and a sphere supported by the base. The sphere includes a spherical tank cover and a cavity. The cavity is divided into an upper explosion zone and a lower water storage zone by a hard explosion relief plate. The explosion zone includes two dust pans and two nozzles, each dust pan corresponds to one nozzle. The water storage zone is used to store bacterial colonies and nutrient solution. After air is supplied and blown, dust is diffused inside the cavity and ignited by the ignition module to achieve a dust explosion. The cavity environment after the explosion serves as the growth environment of the bacterial colonies in the water storage zone. The water supply module includes a water pump and a water inlet pipe connected by pipelines, and water is supplied to the water storage area of ​​the cavity module through the water pump and the water inlet pipe; The temperature control module includes a temperature control layer formed by a sphere and an insulation layer wrapped around the outer surface of the sphere, as well as a temperature controller and a heating wire. The temperature controller controls the heating wire to heat and control the deionized water in the temperature control layer, so that the spatial temperature of the cavity module can be controlled; The ignition module includes an ignition energy generator and two ignition electrodes connected to each other; The data acquisition module includes a main controller, a temperature and pressure signal collector, a temperature controller, a water supply and gas supply controller, a pressure sensor, a temperature sensor and a camera. The temperature and pressure signal collector transmits the signals collected from the pressure sensor and the temperature sensor to the main controller, and the main controller also receives signals transmitted from the temperature controller, the water supply and gas supply controller.

2. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment according to claim 1, characterized in that: The cavity is connected to the water supply module, and the water is located in the water storage area. The water storage area is provided with a filter membrane for achieving separation between water and bacterial colonies.

3. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment according to claim 1, characterized in that: The water storage area is connected to a liquid level meter for observing water level changes.

4. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment according to claim 1, characterized in that: A sampling port is provided outside the cavity, and the position where the sampling port is connected to the cavity is located above the filter membrane.

5. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment as claimed in claim 1, characterized in that: The spherical tank cover comprises a handle and a flange, and the spherical tank cover is connected to the cavity through bolts on the flange.

6. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment according to claim 5, characterized in that: The ignition electrodes pass through the spherical tank cover. After the spherical tank cover is installed on the cavity, the two ignition electrodes are located in the middle of the cavity, and the distance between the two ignition electrodes is no more than 6 mm.

7. The experimental device for simulating the effect of dust explosion on bacterial growth in a confined environment as claimed in claim 1, characterized in that: The cavity is provided with a visual window, and the visual window is located at the water storage area. The liquid level of the water storage area is located at the horizontal center of the visual window, and the shooting machine collects pictures through the visual window.

8. An experimental method for the effect of dust explosion on bacterial colony growth in a confined environment, using the simulation experimental apparatus according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) Assemble the experimental apparatus; (2) Weigh the dust and place it in a dust pan; (3) Turn on the thermostat and adjust the temperature; (4) Turn on the water pump to supply water to the water storage area, and add bacterial colonies and nutrient solution into the water; (5) Install the hard explosion venting disc; (6) Cover the spherical tank cover and tighten it with bolts; (7) Turn on the vacuum pump to extract air; (8) Turn on the air compressor to supply air to the air storage chamber. When the pressure is reached, turn off the air compressor; (9) Setting the ignition energy value and ignition delay time of the ignition energy generator; (10) The main controller opens the solenoid valve, and the air in the air storage chamber lifts the dust through the nozzle to form a dust cloud; (11) After the ignition delay time, the ignition electrode discharges and ignites the dust cloud. After the dust is successfully ignited, the shock wave breaks the hard explosion venting membrane, and the air and reactants after the dust explosion fill the cavity module; (12) The colonies in the water storage area survive in the post-explosion environment. The colony production is observed through the visual window and the filming machine is used to photograph the colony, and the colonies are sampled and observed through the sampling port.

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