Oral fish oil microemulsion for animals and preparation method thereof

Abstract

The present application belongs to the field of fish oil technology, and in particular, relates to an oral fish oil microemulsion for animals and a preparation method thereof. Among them, the oral fish oil microemulsion for animals includes raw material components: 220-460 parts of fish oil, 0.3-5.2 parts of vitamins, 20-77 parts of emulsifiers, 15.3-43.4 parts of stabilizers, 139.6-273 parts of attractants and 141.4-604.8 parts of water. The oral fish oil microemulsion for animals of the present application fully emulsifies and fuses the raw material components through an emulsification process to form an oral fish oil microemulsion dosage form for animals that is uniformly mixed, stable in physical properties, and good in palatability, which effectively improves the palatability of pets. In addition to the main components of fish oil, the oral fish oil microemulsion for animals also contains vitamins, stabilizers, attractants and other ingredients, which can not only improve the storage and transportation stability of the oral fish oil microemulsion for animals, but also enrich the types of nutrients in the oral fish oil microemulsion for animals, further improve the palatability of animals, and are more conducive to the active eating of pets.

Description

Technical Field

[0001] The present application belongs to the technical field of fish oil, and in particular relates to an oral fish oil microemulsion for animals and a preparation method thereof. Background Art

[0002] Fish oil is a kind of oil extracted from fatty fish and is a kind of nutritious food. In the growth process of dogs and cats, fish oil plays a vital role in the skin, hair, cardiovascular and cerebrovascular aspects. Therefore, when feeding pets, you can add fish oil ingredients in moderation.

[0003] Most of the fish oils currently available on the market are in the form of capsules or drops. Due to their poor palatability, they can only be force-fed. Moreover, since most of the nutrients are difficult to dissolve in oil, most of the fish oil products on the market are just single fish oils with single nutrients. Summary of the invention

[0004] The purpose of the present application is to provide an oral fish oil microemulsion for animals and a preparation method thereof, which aims to solve the problem that the existing oral fish oil for animals is mostly in the form of capsules or drops, has poor palatability, can only be force-fed during feeding, and has a single nutritional component.

[0005] In order to achieve the above application purpose, the technical solution adopted in this application is as follows:

[0006] In a first aspect, the present application provides an oral fish oil microemulsion for animals, which comprises raw material components: 220 to 460 parts of fish oil, 0.3 to 5.2 parts of vitamins, 20 to 77 parts of emulsifiers, 15.3 to 43.4 parts of stabilizers, 139.6 to 273 parts of attractants and 141.4 to 604.8 parts of water.

[0007] In a second aspect, the present application provides a method for preparing an oral fish oil microemulsion for animals, comprising the following steps:

[0008] Mixing 20 to 77 parts of an emulsifier, 220 to 460 parts of fish oil, and 0.3 to 5.2 parts of vitamins with water to form a first mixed slurry;

[0009] Mixing 15.3 to 43.4 parts of a stabilizer, 139.6 to 273 parts of an attractant, and water to form a second mixed slurry;

[0010] The first mixed slurry and the second mixed slurry are mixed and ground, and sterilized to obtain an oral fish oil microemulsion for animals.

[0011] The oral fish oil microemulsion for animals provided in the first aspect of the present application is a raw material component of 220-460 parts of fish oil, 0.3-5.2 parts of vitamins, 20-77 parts of emulsifiers, 15.3-43.4 parts of stabilizers, 139.6-273 parts of attractants and 141.4-604.8 parts of water, which is fully integrated through an emulsification process to form an oral fish oil microemulsion formulation for animals that is uniformly mixed, stable in physical properties and good in palatability, which effectively improves the palatability of pets. In addition, in addition to the main component of fish oil, the oral fish oil microemulsion for animals also contains components such as vitamins, stabilizers, and attractants, which can not only improve the storage and transportation stability of the oral fish oil microemulsion for animals, but also enrich the types of nutrients in the oral fish oil microemulsion for animals, further improve the palatability of animals, and are more conducive to the active eating of pets.

[0012] The second aspect of the present application provides a method for preparing an oral fish oil microemulsion for animals. According to the performance of the raw material components, 20 to 77 parts of emulsifier, 220 to 460 parts of fish oil and 0.3 to 5.2 parts of vitamins are dissolved / dispersed in water to form a first mixed slurry, and 15.3 to 43.4 parts of stabilizer and 139.6 to 273 parts of attractant are dissolved / dispersed in water to form a second mixed slurry; then the first mixed slurry and the second mixed slurry are mixed and ground to make the raw material components fully mixed and emulsified to form an oral fish oil microemulsion formulation for animals with uniform mixing, stable physical properties and good palatability. The prepared oral fish oil microemulsion for animals has rich nutritional substances, good storage and transportation stability, effectively improves the palatability of pets, and is more conducive to the active eating of pets. In addition, the preparation process is simple and suitable for industrial large-scale production and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0014] Figure 1 1 is a schematic diagram of a method for preparing an oral fish oil microemulsion for animals provided in an embodiment of the present application;

[0015] Figure 2 It is a schematic flow chart of the preparation method of oral fish oil microemulsion for animals provided in Examples 1 to 6 of the present application. DETAILED DESCRIPTION

[0016] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0017] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can mean: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural. The character " / " generally indicates that the associated objects are in an "or" relationship.

[0018] In this application, "at least one" means one or more, and "plurality" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single items or plural items. For example, "at least one of a, b or c", or "at least one of a, b and c", can all represent: a, b, c, ab (i.e. a and b), ac, bc, or abc, where a, b, c can be single or multiple, respectively.

[0019] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0020] The terms used in the embodiments of the present application are only for the purpose of describing specific embodiments, and are not intended to limit the present application. The singular forms "a", "an" and "the" used in the embodiments of the present application and the appended claims are also intended to include plural forms, unless the context clearly indicates other meanings.

[0021] The weight of the relevant components mentioned in the embodiments of the present specification can not only refer to the specific content of each component, but also represent the proportional relationship between the weights of the components. Therefore, as long as the content of the relevant components in the embodiments of the present specification is proportionally enlarged or reduced, it is within the scope disclosed in the embodiments of the present specification. Specifically, the mass in the embodiments of the present specification can be μg, mg, g, kg and other mass units known in the chemical industry.

[0022] The terms "first" and "second" are used only for descriptive purposes to distinguish objects such as substances from each other, and should not be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. For example, without departing from the scope of the embodiments of the present application, the first XX may also be referred to as the second XX, and similarly, the second XX may also be referred to as the first XX. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0023] The first aspect of the embodiment of the present application provides an oral fish oil microemulsion for animals, which includes raw material components: 220 to 460 parts of fish oil, 0.3 to 5.2 parts of vitamins, 20 to 77 parts of emulsifiers, 15.3 to 43.4 parts of stabilizers, 139.6 to 273 parts of attractants and 141.4 to 604.8 parts of water.

[0024] The oral fish oil microemulsion for animals provided in the first aspect of the embodiment of the present application is a method of fully blending raw material components such as 220 to 460 parts of fish oil, 0.3 to 5.2 parts of vitamins, 20 to 77 parts of emulsifiers, 15.3 to 43.4 parts of stabilizers, 139.6 to 273 parts of attractants, and 141.4 to 604.8 parts of water through an emulsification process to form an oral fish oil microemulsion formulation for animals that is uniformly mixed, stable in physical properties, and good in palatability, which effectively improves the palatability of pets. In addition, in addition to the main component of fish oil, the oral fish oil microemulsion for animals also contains components such as vitamins, stabilizers, and attractants, which can not only improve the storage stability of the oral fish oil microemulsion for animals, but also enrich the types of nutrients in the oral fish oil microemulsion for animals, further improve the palatability of animals, and are more conducive to the active eating of pets.

[0025] In some embodiments, the fish oil in the oral fish oil microemulsion for animals is imported deep-sea fish oil, which is rich in various unsaturated fatty acids such as docosahexaenoic acid DHA and eicosapentaenoic acid EPA, and has the effects of helping to reduce the content of cholesterol and triglycerides and promoting the metabolism of saturated fatty acids in the body. It has the effects of reducing blood viscosity, promoting blood circulation, improving tissue oxygen supply and eliminating fatigue; preventing the deposition of fat in blood vessel walls, etc. At the same time, it can assist brain cell development, anti-aging effects, improve blood circulation, and reduce blood lipids.

[0026] In some embodiments, the vitamins include vitamin A and vitamin E; vitamin A, in addition to protecting visual function, also has multiple physiological functions such as promoting growth and reproduction, maintaining normal secretion of bones, epithelial tissues and mucosal epithelium. Vitamin E has strong antioxidant properties and removes free radicals from the body. In addition to being nutritional supplements, vitamins A and E also have strong antioxidant properties. Therefore, both vitamins can protect fish oil and avoid oxidation.

[0027] In some embodiments, the mass ratio of vitamin A to vitamin E is (0.2-2.4): (0.1-2.8); this ratio of vitamin A and vitamin E can not only enrich the nutritional components of the oral fish oil microemulsion for animals, but also help protect the active ingredients in the oral fish oil microemulsion for animals from oxidation, thereby improving the storage stability of the oral fish oil microemulsion for animals.

[0028] In some embodiments, the emulsifier includes citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum and gum arabic; wherein, citric acid fatty acid glyceride is a non-ionic food emulsifier, which is a product of esterification of glycerol with fatty acids and citric acid, with an HLB value of 4-6, and has the functions of emulsification, dispersion, chelation, antioxidant enhancement, anti-starch aging and control of fat aggregation. Soluble soybean polysaccharide has good dispersion stability, and in an acidic system with a low pH value, it prevents the precipitation of other tiny solid particles such as protein molecules, thereby improving the stability of oral fish oil microemulsion for animals; and it has extremely high solubility, is soluble in warm water and cold water, does not form gel, has good heat resistance and acid resistance, and its stability effect is almost unaffected by salts. At the same time, soluble soybean polysaccharide has a high dietary fiber content, has the physiological functions of water-soluble dietary fiber such as adjusting intestinal function, and can be used as a dietary raw material for fiber supplements. Both flaxseed gum and gum arabic have emulsification stability. Compared with gum arabic, the emulsification stability of flaxseed gum is lower than that of gum arabic, and the emulsion formed is not as stable as the emulsion formed by gum arabic. Citric acid fatty acid glyceride, soluble soybean polysaccharide, flaxseed gum and gum arabic are all conducive to the emulsification of each raw material component to form an animal oral fish oil microemulsion with stable physical and chemical properties.

[0029] In some embodiments, in the emulsifier, the mass ratio of citric acid fatty acid glyceride, soluble soybean polysaccharide, flaxseed gum and gum arabic is (8-12):(15-25):(10-13):(12-16).

[0030] In some embodiments, the stabilizer includes galactomannan and tri-glucon; wherein galactomannan is a low-polymerization galactomannan made from endosperm slices or gums of guar, sesbania, coumarin, cassia seed, and locust bean, processed by enzymolysis, purification, and other steps with one or more enzymes such as galactosidase, hemicellulase, and protease, and is composed of D-mannose connected by β-1,4 glycosidic bonds to form a main chain, and D-galactose on some mannose forms a side chain through α-1,6 glycosidic bonds to form a multi-branched polysaccharide, which belongs to a polysaccharide polymer compound. Tri-glucon is composed of monosaccharides of glucose, mannose, rhamnose, and glucuronic acid, and lipids are composed of 16-carbon and 18-carbon fatty acids; it contains a sugar skeleton similar to gellan gum and an amino acid composition of 23.3% of Asp and Glu, and has thickening, gel-forming properties, and excellent stability.

[0031] In some embodiments, in the stabilizer, the mass ratio of galactomannan to trisammonia gum is 1:(17.08-50). The synergistic effect is produced by optimizing the ratio between galactomannan and trisammonia gum to achieve a given rheological property, which can effectively improve the stability of oral fish oil microemulsion for animals. In some preferred embodiments, the mass ratio of galactomannan to trisammonia gum in the stabilizer includes but is not limited to 1:(17-20), 1:(20-30), 1:(30-40), 1:(40-50), etc.

[0032] In some embodiments, the attractant includes inulin, fructose syrup, trehalose and flavored chicken liver peptide; wherein, inulin uses natural inulin imported from Belgium, which is a naturally occurring fructan, an excellent fat substitute, with a neutral taste, can mask bitterness and give a soft feeling, and can provide a smooth taste, a well-balanced and rounded flavor when fully mixed with water; at the same time, it can improve digestive function, relieve constipation, maintain intestinal microecological balance, and promote the absorption of minerals (such as calcium and magnesium). The main components and properties of fructose syrup are close to those of natural fruit juice, with a fruity fragrance. In terms of taste, the taste sweetness is stronger than sucrose, and it has a cool feeling. It has the characteristics of high solubility, good crystallization resistance, good moisture retention, high osmotic pressure, good fermentation performance, good anti-caries performance, and high chemical stability. Trehalose is a natural sugar that can inhibit the generation of fishy smell. During the processing of fish food, especially during heating treatment, trimethylamine (fishy smell component) will be produced. If trehalose is added before heating, the formation of trimethylamine can be inhibited and the generation of unpleasant odor can be reduced; at the same time, trehalose is a typical stress metabolite, which can form a unique protective film on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure and dehydration, effectively protecting the biological molecular structure from being destroyed, thereby maintaining the life process and biological characteristics of living organisms. Trehalose is a proliferation factor for beneficial intestinal bacteria-bifidobacteria in the body. It can improve the intestinal microecological environment, strengthen the digestion and absorption function of the gastrointestinal tract, effectively eliminate toxins in the body, and enhance the body's immune resistance. Studies have also shown that trehalose has a strong anti-radiation effect. Flavored chicken liver peptide not only has a seasoning function, but also can supplement a variety of trace elements. Inulin, fructose syrup, trehalose and flavored chicken liver peptide are all attractants that are beneficial to improving the palatability of animals to oral fish oil microemulsion, improving animal appetite, and more conducive to the intake of fish oil.

[0033] In some embodiments, in the attractant, the mass ratio of inulin, fructose syrup, trehalose and flavored chicken liver peptide is 1:(40-50):(40-50):(10-20).

[0034] In some embodiments, the oral fish oil microemulsion for animals includes raw material components: 220-460 parts of fish oil, 0.1-2.8 parts of vitamin E, 0.2-2.4 parts of vitamin A, 20-77 parts of emulsifier, 15.3-43.4 parts of stabilizer, 139.6-273 parts of attractant and 141.4-604.8 parts of water. The ratio of each raw material component in the oral fish oil microemulsion for animals in the embodiment of the present application can effectively improve the stability, animal palatability, nutritional ingredients, emulsification effect, animal appetite and other comprehensive properties of the oral fish oil microemulsion for animals.

[0035] Furthermore, in some embodiments, the oral fish oil microemulsion for animals includes the following raw material components: 270-430 parts of fish oil, 0.3-1.6 parts of vitamin E, 0.4-1.3 parts of vitamin A, 29.5-64 parts of emulsifier, 22.5-35.7 parts of stabilizer, 166.3-240.2 parts of attractant and 227.2-511 parts of water. The oral fish oil microemulsion for animals with this ratio has better comprehensive properties such as stability, animal palatability, nutritional ingredients, emulsification effect, and animal appetite.

[0036] In some specific embodiments, the oral fish oil microemulsion for animals includes the following raw material components: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 55 parts of emulsifier, 31 parts of stabilizer, 212 parts of attractant and 300 parts of water.

[0037] Furthermore, in some specific embodiments, the oral fish oil microemulsion for animals includes the following raw material components: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide and 300 parts of water.

[0038] In some embodiments, the fish oil used in the embodiments of the present application meets the requirements of SC / T3504-2006 fish oil for feed. Vitamin E meets the requirements of GB9454-2017 feed additive DL-α-tocopheryl acetate. Vitamin A meets the requirements of GB / T21123-2007 nutritional fortified vitamin A edible oil. Citric acid fatty acid glycerides meet the requirements of GB29951-2013 food safety national standard food additive citric acid fatty acid glycerides. Soluble soybean polysaccharides meet the requirements of GB1886.322-2021 food safety national standard food additive soluble soybean polysaccharides. Flaxseed gum meets the requirements of GB1886.175-2016 food safety national standard food additive flaxseed gum. Gum arabic meets the requirements of GB29949-2013 food safety national standard food additive arabic gum. Galactomannan meets the requirements of GB1886.301-2018 food safety national standard food additive galactomannan. Sanzan gum meets the requirements of Sanzan gum in the "Announcement on 53 "Three New Foods" Including Lactobacillus helveticus R0052 (No. 4 of 2020)", and inulin meets the requirements of the Ministry of Health No. 5 of 2009. Fructose syrup meets the requirements of GB / T20882.4-2021 Starch Sugar Quality Requirements Part 4: Fructose syrup. Trehalose meets the requirements of GB / T23529-2009 Trehalose.

[0039] In some embodiments, the particle size Dv50 of the fish oil microemulsion for oral administration to animals is 2.54-6.32 μm. The particle size affects stability and absorption, and this particle size is conducive to absorption.

[0040] As attached Figure 1 As shown, the second aspect of the embodiment of the present application provides a method for preparing fish oil microemulsion for oral administration to animals, comprising the following steps:

[0041] S10. Mixing 20 to 77 parts of an emulsifier, 220 to 460 parts of fish oil and 0.3 to 5.2 parts of vitamins with water to form a first mixed slurry;

[0042] S20. 15.3 to 43.4 parts of a stabilizer, 139.6 to 273 parts of an attractant and water are mixed to form a second mixed slurry;

[0043] S30. The first mixed slurry and the second mixed slurry are mixed and ground, and sterilized to obtain an oral fish oil microemulsion for animals.

[0044] The preparation method of the oral fish oil microemulsion for animals provided in the second aspect of the embodiment of the present application is, according to the performance of the raw material components, 20 to 77 parts of emulsifier, 220 to 460 parts of fish oil and 0.3 to 5.2 parts of vitamins are dissolved / dispersed in water to form a first mixed slurry, and 15.3 to 43.4 parts of stabilizer and 139.6 to 273 parts of attractant are dissolved / dispersed in water to form a second mixed slurry; then the first mixed slurry and the second mixed slurry are mixed and ground to make the raw material components fully mixed, fused and emulsified to form an oral fish oil microemulsion formulation for animals with uniform mixing, stable physical properties and good palatability. The prepared oral fish oil microemulsion for animals has rich nutrients and good storage stability, which effectively improves the palatability of pets and is more conducive to the active eating of pets. In addition, the preparation process is simple and suitable for industrial large-scale production and application.

[0045] In some embodiments, in the above step S10, the step of mixing the emulsifier, fish oil and vitamins with water comprises:

[0046] S11. Add the emulsifier to water at a temperature of 55 to 80°C, stir for 5 to 20 minutes at a rotation speed of 20 to 70 rpm, shear for 7 to 45 minutes at a rotation speed of 1000 to 3000 rpm, and grind for 1 to 3 times at a rotation speed of 10000 to 16000 rpm; add fish oil and vitamins, and shear for 8 to 45 minutes at a rotation speed of 1000 to 3000 rpm to obtain a first mixed slurry. Among them, the temperature conditions, mixing speed, shearing speed, grinding speed, processing time and other parameters fully ensure that the emulsifier, fish oil and vitamins are mixed evenly with water to form a first mixed slurry with stable physical properties.

[0047] In some embodiments, in the above step S20, the step of mixing the stabilizer, the attractant and the water comprises:

[0048] S21. Add the stabilizer and the attractant to water at a temperature of 65 to 85°C, stir at a rotation speed of 20 to 80 rpm for 15 to 45 minutes, and then shear at a rotation speed of 1000-3000 rpm for 7 to 15 minutes to allow the stabilizer and the attractant to be fully, evenly and stably dissolved / dispersed in the water to obtain a second mixed slurry.

[0049] In some embodiments, the ratio of the amount of water in the first mixed slurry to the amount of water in the second mixed slurry is (2-3):1; this ratio of water fully ensures that each raw material component can be fully dissolved / dispersed in water to form a mixed slurry with uniform mixing and stable physical properties.

[0050] In some embodiments, in the above step S30, the mixing and grinding process includes: stirring for 5 to 10 minutes at a rotation speed of 20 to 80 rpm, shearing for 7 to 25 minutes at a rotation speed of 1000 to 3000 rpm, and grinding for 1 to 3 times at a rotation speed of 10000 to 16000, so that the raw material components in the first mixed slurry and the second mixed slurry are fully integrated to form a mixed slurry with uniform mixing and stable physical properties; and then homogenizing for 1 to 6 times at a pressure of 7 to 25 MPa to fully emulsify the mixed slurry to form an oral fish oil microemulsion for animals.

[0051] In some embodiments, the sterilization temperature is 110-125° C. and the duration is 15-40 minutes. Such sterilization conditions can effectively kill harmful microorganisms in the oral fish oil microemulsion for animals and improve the safety and storage stability of the oral fish oil microemulsion for animals.

[0052] In some embodiments, the vitamins include vitamin A and vitamin E in a mass ratio of (0.2-2.4): (0.1-2.8);

[0053] In some embodiments, the emulsifier includes citric acid fatty acid glyceride, soluble soybean polysaccharide, flaxseed gum and gum arabic in a mass ratio of (8-12): (15-25): (10-13): (12-16);

[0054] In some embodiments, the stabilizer includes galactomannan and trisammonium gum in a mass ratio of 1:(17.08-50);

[0055] In some embodiments, the phagostimulant comprises inulin, fructose syrup, trehalose and flavored chicken liver peptide in a mass ratio of 1:(40-50):(40-50):(10-20);

[0056] The technical effects of the above embodiments of the present application have been discussed in detail in the previous text and will not be repeated here.

[0057] In some embodiments, the preparation of flavored chicken liver peptide comprises the steps of:

[0058] S40. Mashing the chicken liver and adding water to prepare chicken liver slurry;

[0059] S50. Mixing chicken liver slurry with trypsin and ficin at a temperature of 50 to 60°C and keeping the mixture warm for 100 to 150 minutes; heating the mixture to 90 to 100°C and keeping the mixture warm for 60 to 80 minutes; wherein the chicken liver slurry and trypsin and ficin have an enzymatic effect of enhancing the flavor;

[0060] S60. After adding yeast extract, glucose and L-cysteine, the mixture is heated to 100-110° C. and kept warm for 100-120 min; wherein the yeast extract, glucose and L-cysteine ​​act as flavor enhancers and salty flavors;

[0061] S70. Add phosphoric acid and maltodextrin, mix, grind and sieve, collect the filtrate, and obtain flavored chicken liver peptide. Phosphoric acid and maltodextrin play a role in adjusting pH value and thickening.

[0062] In some embodiments, in the above step S40, the chicken liver is minced into chicken liver mince, 0.5 to 1.5 times the weight of the chicken liver mince is added with water to stir into chicken liver slurry, and the chicken liver slurry is heated to 60° C. for use.

[0063] In some embodiments, in the above step S50, 1-2% trypsin and 1-3% ficin are added to the chicken liver slurry by weight, and the mixture is kept warm at 50-60°C for 100-140 minutes under stirring, and then the mixture is heated to 90-100°C and kept warm for 60-80 minutes under stirring. In some embodiments, the enzyme activities of trypsin and ficin are not less than 100,000 u / g.

[0064] In some embodiments, in the above step S60, 6-8% yeast extract, 8-12% glucose, and 1-3% L-cysteine ​​are added to the chicken liver slurry by weight, and then the mixture is heated to 100-110° C. and kept warm for 100-120 min.

[0065] In some embodiments, in the above step S70, 4-6% phosphoric acid and 8-12% maltodextrin are added to the chicken liver slurry by weight, stirred evenly, ground with a colloid mill for 1-3 times, passed through an 80-150 mesh sieve, and the filtrate is collected to obtain flavored chicken liver peptide.

[0066] In some embodiments, the materials used to prepare flavored chicken liver peptides preferably meet the following standards: chicken liver should meet the requirements of GB16869 and GB 2707; trypsin and ficin should meet the requirements of GB / T 23527; phosphoric acid should meet the requirements of GB1886.15; maltodextrin should meet the requirements of GB / T 20882.6; yeast extract should meet the requirements of GB / T 20886.2; glucose should meet the requirements of GB / T 20880; L-cysteine ​​should meet the requirements of GB 2760.

[0067] In order to make the above implementation details and operations of the present application clearly understood by those skilled in the art, and to demonstrate the significant improvement in the performance of the oral fish oil microemulsion for animals and the preparation method thereof in the embodiments of the present application, the above technical scheme is illustrated by multiple embodiments below.

[0068] Examples 1 to 6

[0069] Examples 1 to 6 respectively provide a fish oil microemulsion for oral administration to animals, and the raw material component formulas thereof are shown in Table 1 below, wherein the preparation of the flavored chicken liver peptide comprises the following steps:

[0070] 1. Crush the chicken liver into minced chicken liver, add 1 times the weight of the minced chicken liver into water and stir to make chicken liver slurry;

[0071] 2. At a temperature of 60°C, add 1% trypsin and 2% ficin to the material of step (1), keep warm at 60°C for 120 minutes with stirring, raise the temperature to 90°C, keep warm for 60 minutes with stirring, wherein the enzyme activities of trypsin and ficin are 100,000 u / g;

[0072] 3. Add 7% yeast extract, 10% glucose and 2% L-cysteine ​​by weight of chicken liver paste to the material in step (2), mix them, and heat to 110° C. for 120 min.

[0073] 4. Add 5% phosphoric acid and 10% maltodextrin by weight of chicken liver minced to the material in step (3), stir evenly, grind twice with a colloid mill, pass through a 100-mesh sieve, collect the filtrate, and obtain flavored chicken liver peptide.

[0074] The schematic diagram of the preparation method of fish oil microemulsion for oral administration to animals is shown in the attached figure. Figure 2 As shown, the steps include:

[0075] 1. Heat 2 / 3 of the water to 65 degrees Celsius, add emulsifier and stir at 40 rpm for 12 minutes, then shear at 2000 rpm for 25 minutes, and grind twice at 12000 rpm to obtain material i; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0076] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add stabilizer and attractant, stir at 50 rpm for 25 minutes, and then shear at 2000 rpm for 10 minutes to obtain the second mixed slurry;

[0077] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2000 rpm for 18 minutes; grind twice at 14000 rpm, and then homogenize twice at 15 MPa, and sterilize at 110 degrees Celsius for 40 minutes to obtain oral fish oil microemulsion for animals.

[0078] Table 1

[0079]

[0080] Example 7

[0081] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0082] The preparation of fish oil microemulsion for oral administration to animals includes the following steps:

[0083] 1. Heat 2 / 3 of the water to 55 degrees Celsius, add emulsifier and stir at 20 rpm for 5 minutes, then shear at 1000 rpm for 7 minutes, and grind once at 16000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 3000 rpm for 8 minutes to obtain the first mixed slurry;

[0084] 2. Heat 1 / 3 of the water to 65 degrees Celsius, add stabilizer and attractant, stir at 20 rpm for 15 minutes, and then shear at 1000 rpm for 7 minutes to obtain the second mixed slurry;

[0085] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 20 rpm for 5 min, and then shear at 1000 rpm for 25 min; grind at 10000 rpm for 3 times, and then homogenize at 7 MPa for 6 times, and sterilize at 125 degrees Celsius for 15 minutes to obtain oral fish oil microemulsion for animals.

[0086] Example 8

[0087] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0088] The preparation of fish oil microemulsion for oral administration to animals includes the following steps:

[0089] 1. Heat 2 / 3 of the water to 80 degrees Celsius, add emulsifier and stir at 70 rpm for 20 minutes, then shear at 3000 rpm for 45 minutes, and grind at 10000 rpm for 3 times; then add fish oil, vitamin A and vitamin E, and shear at 1000 rpm for 45 minutes to obtain the first mixed slurry;

[0090] 2. Heat 1 / 3 of the water to 85 degrees Celsius, add stabilizer and attractant, stir at 80 rpm for 45 minutes, and then shear at 3000 rpm for 15 minutes to obtain the second mixed slurry;

[0091] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 80 rpm for 10 min, and then shear at 3000 rpm for 7 min; grind once at 16000 rpm, and then homogenize once at 25 MPa, and sterilize at 120 degrees Celsius for 25 minutes to obtain oral fish oil microemulsion for animals.

[0092] Example 9

[0093] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0094] The preparation of fish oil microemulsion for oral administration to animals includes the following steps:

[0095] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add emulsifier and stir at 45 rpm for 12 minutes, then shear at 1800 rpm for 30 minutes, and grind twice at 14000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 28 minutes to obtain the first mixed slurry;

[0096] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add stabilizer and attractant, stir at 50 rpm for 30 minutes, and then shear at 1500 rpm for 12 minutes to obtain the second mixed slurry;

[0097] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 55 rpm for 8 minutes, and then shear at 2200 rpm for 18 minutes; grind twice at 12000 rpm, and then homogenize four times at 12 MPa, and sterilize at 121 degrees Celsius for 15 minutes to obtain oral fish oil microemulsion for animals.

[0098] Example 10

[0099] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0100] The preparation of fish oil microemulsion for oral administration to animals includes the following steps:

[0101] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum, and gum arabic, stir at 40 rpm for 15 minutes, then shear at 1500 rpm for 30 minutes, and grind twice at 15000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0102] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add galactomannan, trisammonium gum, inulin, fructose syrup, trehalose, and flavored chicken liver peptide, stir at 50 rpm for 30 min, and then shear at 1800 rpm for 15 min to obtain a second mixed slurry;

[0103] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind once at 15000 rpm, and then homogenize 3 times at 13 MPa, and sterilize at 110 degrees Celsius for 30 minutes to obtain oral fish oil microemulsion for animals.

[0104] Examples 11 to 16

[0105] Examples 11 to 16 respectively provide a fish oil microemulsion for oral administration to animals, and the raw material component formulas thereof are shown in Table 2 below, wherein the preparation steps of the flavored chicken liver peptide are the same as those of Example 1.

[0106] The method for preparing the oral fish oil microemulsion for animals comprises the following steps:

[0107] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum, and gum arabic, stir at 40 rpm for 15 minutes, then shear at 1500 rpm for 30 minutes, and grind twice at 15000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0108] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add galactomannan, trisammonium gum, inulin, fructose syrup, trehalose, and flavored chicken liver peptide, stir at 50 rpm for 30 min, and then shear at 1800 rpm for 15 min to obtain a second mixed slurry;

[0109] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind once at 15000 rpm, and then homogenize 4 times at 13 MPa, and sterilize at 110 degrees Celsius for 30 minutes to obtain oral fish oil microemulsion for animals.

[0110] Table 2

[0111]

[0112] Embodiments 17 to 19

[0113] Examples 17 to 19 respectively provide a fish oil microemulsion for oral administration to animals, and the raw material component formulas thereof are shown in Table 3 below, wherein the preparation steps of the flavored chicken liver peptide are the same as those of Example 1.

[0114] The method for preparing the oral fish oil microemulsion for animals comprises the following steps:

[0115] 1. Heat 2 / 3 of the water to 65 degrees Celsius, add emulsifier and stir at 40 rpm for 12 minutes, then shear at 2000 rpm for 25 minutes, and grind twice at 12000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0116] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add stabilizer and attractant, stir at 50 rpm for 25 minutes, and then shear at 2000 rpm for 10 minutes to obtain the second mixed slurry;

[0117] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind twice at 14000 rpm, and then homogenize twice at 15 MPa, and sterilize at 110 degrees Celsius for 40 minutes to obtain oral fish oil microemulsion for animals.

[0118] Embodiment 20

[0119] An oral fish oil microemulsion for animals, the raw material component formula of which is shown in Table 3 below:

[0120] Preparation of flavored chicken liver peptides:

[0121] (1) Crush the chicken liver into minced chicken liver, add 1 times the weight of the chicken liver minced water and stir to form chicken liver slurry, and heat the chicken liver slurry to 60° C.;

[0122] (2) Add 1% trypsin and 2% ficin to the material in step (1), keep warm at 60°C for 120 minutes with stirring, raise the temperature to 90°C, keep warm for 60 minutes with stirring, wherein the enzyme activities of trypsin and ficin are 100,000 u / g;

[0123] (3) Add 10% glucose and 2% L-cysteine ​​by weight of chicken liver mince to the material in step (2), raise the temperature to 100° C., and maintain for 100 min;

[0124] (4) Add 5% phosphoric acid and 10% maltodextrin by weight of chicken liver mince to the material in step (3), stir evenly, grind twice with a colloid mill, pass through a 100-mesh sieve, collect the filtrate, and obtain flavored chicken liver peptide.

[0125] Preparation of fish oil microemulsion for oral administration to animals:

[0126] 1. Heat 2 / 3 of the water to 65 degrees Celsius, add emulsifier and stir at 40 rpm for 12 minutes, then shear at 2000 rpm for 25 minutes, and grind twice at 12000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0127] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add stabilizer and attractant, stir at 50 rpm for 25 minutes, and then shear at 2000 rpm for 10 minutes to obtain the second mixed slurry;

[0128] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind twice at 14000 rpm, and then homogenize twice at 15 MPa, and sterilize at 110 degrees Celsius for 40 minutes to obtain oral fish oil microemulsion for animals.

[0129] Embodiment 21

[0130] An oral fish oil microemulsion for animals, the raw material component formula of which is shown in Table 3 below:

[0131] Preparation of flavored chicken liver peptides:

[0132] (1) Crush the chicken liver into minced chicken liver, add 1 times the weight of the chicken liver minced water and stir to form chicken liver slurry, and heat the chicken liver slurry to 60° C.;

[0133] (2) Add 1% trypsin and 2% ficin to the material in step (1), keep warm at 50°C for 120 minutes with stirring, raise the temperature to 90°C, keep warm for 60 minutes with stirring, wherein the enzyme activities of trypsin and ficin are 100,000 u / g;

[0134] (3) Add 7% yeast extract and 10% glucose by weight of chicken liver mince to the material in step (2), raise the temperature to 100° C., and maintain for 100 min;

[0135] (4) Add 5% phosphoric acid and 10% maltodextrin by weight of chicken liver mince to the material in step (3), stir evenly, grind twice with a colloid mill, pass through a 100-mesh sieve, collect the filtrate, and obtain flavored chicken liver peptide.

[0136] The method steps for preparing fish oil microemulsion for oral administration to animals are the same as those in Example 20.

[0137] Embodiment 22

[0138] An oral fish oil microemulsion for animals, the raw material component formula of which is shown in Table 3 below:

[0139] Preparation of flavored chicken liver peptides:

[0140] 1) Crush the chicken liver into minced chicken liver, add 1 times the weight of the chicken liver minced water to stir into chicken liver slurry, and heat the chicken liver slurry to 60°C;

[0141] (2) Add 1% trypsin and 2% ficin to the material in step (1), keep warm at 55°C for 120 minutes with stirring, raise the temperature to 90°C, keep warm for 60 minutes with stirring, wherein the enzyme activities of trypsin and ficin are 100,000 u / g;

[0142] (3) Add 7% yeast extract and 2% L-cysteine ​​by weight of chicken liver mince to the material in step (2), raise the temperature to 100° C., and maintain for 100 min;

[0143] (4) Add 5% phosphoric acid and 10% maltodextrin by weight of chicken liver mince to the material in step (3), stir evenly, grind twice with a colloid mill, pass through a 100-mesh sieve, collect the filtrate, and obtain flavored chicken liver peptide.

[0144] The method steps for preparing fish oil microemulsion for oral administration to animals are the same as those in Example 21.

[0145] Table 3

[0146]

[0147]

[0148] Embodiment 23

[0149] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of phospholipids, 1 part of galactomannan, 30 parts of trisammonium gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0150] The preparation of fish oil microemulsion for oral administration to animals includes the following steps:

[0151] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum, and phospholipids, stir at 40 rpm for 15 minutes, then shear at 1500 rpm for 30 minutes, and grind twice at 15000 rpm; then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0152] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add galactomannan, trisammonium gum, inulin, fructose syrup, trehalose, and flavored chicken liver peptide, stir at 50 rpm for 30 min, and then shear at 1800 rpm for 15 min to obtain a second mixed slurry;

[0153] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind once at 15000 rpm, and then homogenize 3 times at 13 MPa, and sterilize at 110 degrees Celsius for 30 minutes to obtain oral fish oil microemulsion for animals.

[0154] Embodiment 24

[0155] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of calcium caseinate, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0156] The steps for preparing the oral fish oil microemulsion for animals are the same as those in Example 23, except that sodium caseinate is used instead of soluble soybean polysaccharide, and gum arabic is used instead of phospholipid.

[0157] Embodiment 25

[0158] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of xanthan gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0159] The steps for preparing the oral fish oil microemulsion for animals are the same as those in Example 23, except that xanthan gum is used instead of triglycerides, and gum arabic is used instead of phospholipids.

[0160] Embodiment 26

[0161] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of oligofructose, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0162] The steps for preparing the oral fish oil microemulsion for animals are the same as those in Example 23, except that oligofructose is used instead of inulin, and gum arabic is used instead of phospholipids.

[0163] Embodiment 27

[0164] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of xylitol, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0165] The steps for preparing the oral fish oil microemulsion for animals are the same as those in Example 23, except that xylitol is used instead of trehalose, and gum arabic is used instead of phospholipids.

[0166] Embodiment 28

[0167] The invention discloses an oral fish oil microemulsion for animals, wherein the raw material formula comprises: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose and 300 parts of water.

[0168] The steps for preparing the oral fish oil microemulsion for animals are the same as those in Example 23, except that: flavored chicken liver peptide is not added, and gum arabic is used instead of phospholipids.

[0169] Embodiment 29

[0170] The invention discloses an oral fish oil microemulsion for animals, wherein the raw material formula comprises: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide and 300 parts of water.

[0171] Preparation of flavored chicken liver peptides:

[0172] (1) Crush the chicken liver into minced chicken liver, add 1 times the weight of the chicken liver minced water and stir to form chicken liver slurry, and heat the chicken liver slurry to 60° C.;

[0173] (2) Add 3% trypsin to the material in step (1), keep warm at 55°C for 120 minutes with stirring, raise the temperature to 90°C, keep warm for 60 minutes with stirring, wherein the enzyme activity of trypsin is 100,000 u / g;

[0174] (3) Add 7% yeast extract, 10% glucose and 2% L-cysteine ​​by weight of chicken liver paste to the material in step (2), raise the temperature to 100° C. and maintain for 100 min;

[0175] (4) Add 5% phosphoric acid and 10% maltodextrin by weight of chicken liver mince to the material in step (3), stir evenly, grind twice with a colloid mill, pass through a 100-mesh sieve, collect the filtrate, and obtain flavored chicken liver peptide.

[0176] Preparation of fish oil microemulsion for oral administration to animals:

[0177] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum, and gum arabic, stir at 40 rpm for 15 minutes, then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0178] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add galactomannan, trisammonium gum, inulin, fructose syrup, trehalose, and flavored chicken liver peptide, stir at 50 rpm for 30 min, and then shear at 1800 rpm for 15 min to obtain a second mixed slurry;

[0179] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind once at 15000 rpm, and then homogenize 3 times at 13 MPa, and sterilize at 110 degrees Celsius for 30 minutes to obtain oral fish oil microemulsion for animals.

[0180] Embodiment 30

[0181] An oral fish oil microemulsion for animals, the raw material formula of which includes: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide, and 300 parts of water. The preparation steps of the flavored chicken liver peptide are the same as those in Example 1.

[0182] Preparation of fish oil microemulsion for oral administration to animals:

[0183] 1. Heat 2 / 3 of the water to 70 degrees Celsius, add citric acid fatty acid glyceride, soluble soybean polysaccharide, linseed gum, and gum arabic, stir at 40 rpm for 15 minutes, then add fish oil, vitamin A and vitamin E, and shear at 2000 rpm for 25 minutes to obtain the first mixed slurry;

[0184] 2. Heat 1 / 3 of the water to 75 degrees Celsius, add galactomannan, trisammonium gum, inulin, fructose syrup, trehalose, and flavored chicken liver peptide, stir at 50 rpm for 30 min, and then shear at 1800 rpm for 15 min to obtain a second mixed slurry;

[0185] 3. After mixing the first mixed slurry and the second mixed slurry, stir at 50 rpm for 8 minutes, and then shear at 2100 rpm for 18 minutes; grind once at 15000 rpm, and then homogenize 3 times at 13 MPa, and sterilize at 110 degrees Celsius for 30 minutes to obtain oral fish oil microemulsion for animals.

[0186] Embodiment 31

[0187] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 1 in that: 0.07 parts of vitamin A is contained.

[0188] Embodiment 32

[0189] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 1 in that: 0.08 parts of vitamin E is contained.

[0190] Embodiment 33

[0191] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 6 in that: 3.2 parts of vitamin E are contained.

[0192] Embodiment 34

[0193] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 6 in that: 6 parts of vitamin A.

[0194] Embodiment 35

[0195] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 1 in that: the amount of galactomannan is 0.25 parts.

[0196] Embodiment 36

[0197] The invention discloses a fish oil microemulsion for oral administration to animals, wherein the raw material formula thereof is different from that of Example 1 in that: the amount of trisammonia gum is 15.5 parts.

[0198] Embodiment 37

[0199] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 6 in that: the amount of galactomannan is 2.5 parts.

[0200] Embodiment 38

[0201] An oral fish oil microemulsion for animals, wherein the raw material formula thereof is different from that of Example 6 in that: the amount of Sanzan glue is 38 parts.

[0202] Furthermore, in order to verify the progress of the embodiments of the present application, the following performance tests were performed:

[0203] 1. Emulsion system stability test:

[0204] The oral fish oil microemulsions prepared in Examples 1 to 16, Example 23, Example 24, Example 25, and Example 30 were respectively placed in 500 ml transparent glass bottles, sterilized, and cooled to room temperature. The treated samples were placed in a 40° C. oven, and the properties were observed on day 0, 3 days, 7 days, 15 days, 30 days, 45 days, 60 days, 90 days, 100 days, 110 days, 120 days, 130 days, 140 days, 150 days, 160 days, 170 days, and 180 days.

[0205] The test results are shown in Table 4 below:

[0206] Table 4

[0207]

[0208] The test results in Table 4 above show that the emulsified systems of the samples of Examples 1 to 3 are stable when placed under accelerated conditions at 40°C for more than 90 days, and it is estimated that the properties are stable when placed at room temperature for 12 months; the emulsified systems of the samples of Examples 4 to 10 are stable when placed under accelerated conditions at 40°C for 120 days, and it is estimated that the properties are stable when placed at room temperature for 18 months; the emulsified systems of the samples of Examples 11 to 16, Example 23, and Example 24 are destroyed and the system is stratified when placed under accelerated conditions at 40°C for 0 to 7 days; the emulsified system of the sample of Example 25 is stratified when placed under accelerated conditions at 40°C for 30 days; the emulsified system of the sample of Example 30 is stratified when placed under accelerated conditions at 40°C for 45 days.

[0209] 2. Particle size test:

[0210] The prepared oral fish oil microemulsion samples of Examples 1 to 10 were respectively taken, and their particle sizes were tested according to the particle size distribution laser diffraction method of GB / T19077-2016, dispersant: water, equipment brand: Malvern. The test results are shown in Table 5 below:

[0211] Table 5

[0212] Serial number Dv50 result (μm) Example 1 5.10 Example 2 4.64 Example 3 4.32 Example 4 2.54 Example 5 4.21 Example 6 3.43 Example 7 4.64 Example 8 6.32 Example 9 4.12 Example 10 3.07

[0213] It can be seen from the test results in Table 5 that the particle size Dv50 of the oral fish oil microemulsion samples for animals prepared in Examples 1 to 10 is 2.54 to 6.32 μm.

[0214] 3. Flavor effect and palatability test:

[0215] Experimental animals: 20 healthy adult domestic cats of similar size and weight were selected as experimental subjects.

[0216] Experimental samples: The oral fish oil microemulsion samples for animals prepared in Example 4, Example 17, Example 18, Example 19, Example 20, Example 21, Example 22, Example 26, Example 27, Example 28 and Example 29 were selected.

[0217] Experimental method: Give each test sample separately, feed twice a day (the test is carried out 30 minutes after feeding the cat food in the morning, and 30 minutes before feeding the cat food in the afternoon), and there is an interval of 3 days between the tests of each sample.

[0218] The cat's food intake M for the two times was recorded separately [M(g) = (M1.am-M2.am) + (M1.pm-M2.pm)]. Where M(g) is the sum of the two feeding amounts; M1.am refers to the amount before morning feeding, M2.am refers to the amount after morning feeding; M1.pm refers to the amount before afternoon feeding, M2.pm refers to the amount after afternoon feeding. The test results are shown in Table 6 below:

[0219] Table 6

[0220] sample Feed intake M(g)(X±s) Example 4 340.25±28.53 Embodiment 17 219.45±35.81 Embodiment 18 204.10±32.68 Embodiment 19 214.95±29.72 Embodiment 20 153.95±31.33 Embodiment 21 163.15±29.15 Embodiment 22 184.75±28.99 Embodiment 26 223.85±28.56 Embodiment 27 220.60±30.72 Embodiment 28 106.90±31.05 Embodiment 29 127.45±28.73

[0221] From the test results in Table 6 above, it can be seen that the experimental animal cats showed a higher appetite and good palatability for the animal oral fish oil microemulsion sample prepared in Example 4 of the present application than the animal oral fish oil microemulsion samples prepared in Examples 17, 18, 19, 20, 21, 22, 26, 27, 28 and 29. Specifically, the experimental animal cats had a food intake M (g) of up to 340.25±28.53 for the animal oral fish oil microemulsion sample prepared in Example 4, and had the best palatability.

[0222] 4. Simulated transportation stability test:

[0223] Test samples: Take samples from Examples 1 to 6, Example 35, Example 36, Example 37, and Example 38;

[0224] Trial period: April to June 2022

[0225] Test method: Each group of samples was randomly divided into 2 parts (Y1 and Y2), sample Y1 was kept in Nanjing, and each group of sample Y2 was sent from Nanjing to Harbin by express, and then sent from Harbin to Nanjing by express again after 60 days; the properties were observed and the viscosity of the emulsion was measured, and the viscosity of each group of samples Y1 and Y2 was measured by rotational viscometer measurement method (model: NDJ-5S, rotor: 1# rotor, speed: 30 rpm, temperature: 25°C, Shanghai Lichen Bangxi Instrument Technology Co., Ltd.) according to GB / T 22235-2008 Determination of liquid viscosity). The test results are shown in Table 7 below:

[0226] Table 7

[0227]

[0228]

[0229] From the test results in Table 7 above, it can be seen that the samples prepared in Examples 1 to 6 of the present application all exhibit good transportation stability. However, the transportation stability of the samples prepared in Examples 35, 36, 37, and 38 is reduced, and stability changes occur during transportation and storage across temperature differences, and product properties and viscosity change, which cannot meet normal quality requirements or feeding.

[0230] 5. Physical and chemical index stability test:

[0231] Test samples: Take samples from Examples 1 to 6, 31, 32, 33, and 34;

[0232] Test method: The oral fish oil microemulsion prepared in each embodiment is placed in a 500ml brown glass bottle, sterilized, and cooled to room temperature. The treated sample is placed in a 40°C constant temperature box, and the light intensity is set to 4500lx±500lx. Samples are taken on the 0th day, 30th day, and 180th day to detect the peroxide value, and the odor is evaluated at the last sample (180 days). The peroxide value is detected according to the provisions of GB 5009.227. The test results are shown in Table 8 below:

[0233] Table 8

[0234]

[0235]

[0236] From the test results in Table 8 above, it can be seen that the samples prepared in Examples 1 to 6 of the present application all showed good storage stability, with small changes in peroxide value, and no odor was found in the samples at the end of the test. However, the samples prepared in Examples 31, 32, 33, and 34 all showed reduced storage stability, significantly increased peroxide value, and produced a rancid smell.

[0237] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. An oral fish oil microemulsion for animals, characterized in that: The oral fish oil microemulsion for animals comprises raw material components: 220-460 parts of fish oil, 0.3-5.2 parts of vitamins, 20-77 parts of emulsifiers, 15.3-43.4 parts of stabilizers, 139.6-273 parts of attractants and 141.4-604.8 parts of water; the fish oil is deep-sea fish oil, which is rich in docosahexaenoic acid and eicosapentaenoic acid and other unsaturated fatty acids; the vitamins include vitamin A and Vitamin E; the emulsifier includes citric acid fatty acid glyceride, soluble soybean polysaccharide, flaxseed gum and gum arabic in a mass ratio of (8-12): (15-25): (10-13): (12-16); the stabilizer includes galactomannan and triglyceride in a mass ratio of 1: (17.08-50); the attractant includes inulin, fructose syrup, trehalose and flavored chicken liver peptide in a mass ratio of 1: (40-50): (40-50): (10-20).

2. The oral fish oil microemulsion for animals according to claim 1, characterized in that: The oral fish oil microemulsion for animals comprises raw material components: 220-460 parts of fish oil, 0.1-2.8 parts of vitamin E, 0.2-2.4 parts of vitamin A, 20-77 parts of emulsifier, 15.3-43.4 parts of stabilizer, 139.6-273 parts of attractant and 141.4-604.8 parts of water.

3. The oral fish oil microemulsion for animals according to claim 2, characterized in that: The oral fish oil microemulsion for animals comprises raw material components: 270-430 parts of fish oil, 0.3-1.6 parts of vitamin E, 0.4-1.3 parts of vitamin A, 29.5-64 parts of emulsifier, 22.5-35.7 parts of stabilizer, 166.3-240.2 parts of attractant and 227.2-511 parts of water.

4. The oral fish oil microemulsion for animals according to claim 3, characterized in that: The oral fish oil microemulsion for animals comprises the following raw material components: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 55 parts of emulsifier, 31 parts of stabilizer, 212 parts of attractant and 300 parts of water.

5. The oral fish oil microemulsion for animals according to claim 3, characterized in that: The oral fish oil microemulsion for animals comprises raw material components: 400 parts of fish oil, 1 part of vitamin E, 1 part of vitamin A, 10 parts of citric acid fatty acid glyceride, 20 parts of soluble soybean polysaccharide, 11 parts of flaxseed gum, 14 parts of gum arabic, 1 part of galactomannan, 30 parts of trisammonia gum, 2 parts of inulin, 90 parts of fructose syrup, 90 parts of trehalose, 30 parts of flavored chicken liver peptide and 300 parts of water.

6. The oral fish oil microemulsion for animals according to claim 3, characterized in that: The particle size Dv50 of the oral fish oil microemulsion for animals is 2.54-6.32 μm.

7. A method for preparing the oral fish oil microemulsion for animals according to any one of claims 1 to 6, characterized in that: The following steps are involved: Mixing 20 to 77 parts of an emulsifier, 220 to 460 parts of fish oil, and 0.3 to 5.2 parts of vitamins with water to form a first mixed slurry; Mixing 15.3 to 43.4 parts of a stabilizer, 139.6 to 273 parts of an attractant, and water to form a second mixed slurry; The first mixed slurry and the second mixed slurry are mixed and ground, and sterilized to obtain an oral fish oil microemulsion for animals.

8. The method for preparing the oral fish oil microemulsion for animals according to claim 7, characterized in that: The step of mixing the emulsifier, the fish oil and the vitamins with water comprises: The emulsifier is added to water at a temperature of 55 to 80° C., stirred at a rotation speed of 20 to 70 rpm for 5 to 20 min, sheared at a rotation speed of 1000 to 3000 rpm for 7 to 45 min, and then ground at a rotation speed of 10000 to 16000 rpm for 1 to 3 times; the fish oil and the vitamin are added, and sheared at a rotation speed of 1000 to 3000 rpm for 8 to 45 min to obtain the first mixed slurry; The step of mixing the stabilizer, the attractant and water comprises: Adding the stabilizer and the attractant to water at a temperature of 65 to 85° C., stirring at a rotation speed of 20 to 80 rpm for 15 to 45 minutes, and shearing at a rotation speed of 1000 to 3000 rpm for 7 to 15 minutes to obtain the second mixed slurry; The ratio of the amount of water in the first mixed slurry to the amount of water in the second mixed slurry is (2-3):1; The mixing and grinding process comprises: stirring for 5 to 10 minutes at a rotation speed of 20 to 80 rpm, shearing for 7 to 25 minutes at a rotation speed of 1000 to 3000 rpm, grinding for 1 to 3 times at a rotation speed of 10000 to 16000, and homogenizing for 1 to 6 times at a pressure of 7 to 25 MPa; The sterilization temperature condition is 110-125° C. and the duration is 15-40 minutes.

9. The method for preparing the oral fish oil microemulsion for animals according to claim 8, characterized in that: The preparation of the flavored chicken liver peptide comprises the following steps: Mash the chicken liver and add water to make chicken liver slurry; Under the condition of temperature of 50-60° C., the chicken liver slurry is mixed with trypsin and ficin and kept warm for 100-150 minutes; the temperature is raised to 90-100° C. and kept warm for 60-80 minutes in the mixed state; After adding yeast extract, glucose and L-cysteine, the mixture was heated to 100-110°C and kept warm for 100-120 minutes; Phosphoric acid and maltodextrin are added and mixed, then ground and sieved, and the filtrate is collected to obtain the flavored chicken liver peptide.

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