Filter sterilization module, cell culture system, and filter sterilization method

Abstract

The application provides a filter sterilization module, a cell culture system and a filter sterilization method, comprising a filter and a temperature detection element, the filter is provided with a first connecting end, a second connecting end and a third connecting end which are communicated with each other, the first connecting end is communicated with a first control valve and a second control valve, the second connecting end is communicated with an input end of a third control valve and a fourth control valve, an output end of the third control valve is communicated with a first hydrophobic valve, the third connecting end is communicated with a fifth control valve and a second hydrophobic valve in sequence, and the temperature detection element is used for detecting the temperature in the filter. The filter can be sterilized without being disassembled from the cell culture system, the risk of secondary pollution in the process of sterilization, transfer and installation of the traditional filter is avoided, damage caused by collision in the process of disassembly, cleaning, sterilization and transfer is avoided, and the reliability and service life of the filter sterilization module and the cell culture system are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of filters, in particular to a filter sterilization module, a cell culture system and a filter sterilization method. BACKGROUND

[0002] In the cell culture process, the filter is one of the main filtering means to ensure the cleanliness of the special gas in the cell culture system, so the filter needs to be sterilized regularly. The traditional filter sterilization method is to disassemble the filter from the cell culture system, clean it, and then sterilize it in a sterilization cabinet. After that, it is sealed and packaged in a sterile breathing bag to the filter installation position for in-situ installation and use. However, there is a risk of secondary pollution during the sterilization and transfer of the filter, and the filter is prone to damage due to bumps during the disassembly, cleaning, sterilization and transfer process. SUMMARY

[0003] Therefore, it is necessary to provide a filter sterilization module, a cell culture system and a filter sterilization method to solve the problems of the risk of secondary pollution during the sterilization and transfer of the filter, and the filter is prone to damage due to bumps during the cleaning and sterilization transfer process.

[0004] The technical scheme is as follows:

[0005] In a first aspect, a filter sterilization module is provided, comprising a filter and a temperature detection element, the filter being provided with a first connection end, a second connection end and a third connection end in communication with each other, the first connection end being in communication with a first control valve and a second control valve, the second connection end being in communication with an input end of a third control valve and a fourth control valve, an output end of the third control valve being in communication with a first trap valve, the third connection end being in communication with a fifth control valve and a second trap valve in sequence, and the temperature detection element being used to detect the temperature in the filter.

[0006] The technical scheme is further described as follows:

[0007] In one embodiment, the filter sterilization module further comprises a sixth control valve, the sixth control valve being in communication with the output end of the third control valve.

[0008] In one embodiment, the filter sterilization module further comprises a seventh control valve, the input end of the seventh control valve being in communication with the first connection end.

[0009] In one embodiment, the output end of the seventh control valve is in communication with the input end of the second trap valve.

[0010] In one of the embodiments, the filter sterilization module further comprises a first straight pipe arranged obliquely, an output end of the first straight pipe is located above an input end of the first straight pipe, the output end of the first straight pipe is in communication with the first connecting end correspondingly, and the input end of the first straight pipe is in communication with the output end of the first control valve, the output end of the second control valve and the input end of the seventh control valve correspondingly.

[0011] In one of the embodiments, the filter sterilization module further comprises a second straight pipe arranged obliquely, an input end of the second straight pipe is located above an output end of the second straight pipe, the input end of the second straight pipe is in communication with the third connecting end correspondingly, and the output end of the second straight pipe is in communication with the input end of the second hydrophobic valve correspondingly.

[0012] In one of the embodiments, the first connecting end is provided with a first pressure detection element for detecting the pressure at the first connecting end, and the second connecting end is provided with a second pressure detection element for detecting the pressure at the second connecting end.

[0013] In a second aspect, a cell culture system is provided, comprising the filter sterilization module.

[0014] In a third aspect, a filter sterilization method is provided, applied to the filter sterilization module, comprising:

[0015] closing the first control valve and the fourth control valve, opening the second control valve, the third control valve and the fifth control valve, and inputting pure steam into the second control valve to discharge the non-condensable gas and the condensed water in the filter, and the temperature in the filter is increased;

[0016] when the temperature value detected by the temperature detection element reaches the preset temperature, timing is started;

[0017] when the timing reaches the preset time, the filter sterilization is completed.

[0018] In one of the embodiments, after the filter sterilization is completed when the timing reaches the preset time, the method further comprises:

[0019] closing the second control valve, stopping inputting pure steam into the second control valve, opening the first control valve, and conveying special gas into the first control valve to purge the residual condensed water in the filter.

[0020] The filter sterilization module, the cell culture system and the filter sterilization method in the above embodiments, when the filter needs to be sterilized, the first control valve and the fourth control valve are closed, the second control valve, the third control valve and the fifth control valve are opened, and pure steam is input into the second control valve, so that the pure steam can blow the non-condensable gas and the condensed water in the filter, and the non-condensable gas and the condensed water in the filter can be discharged through the first hydrophobic valve and the second hydrophobic valve, while the pure steam in the filter cannot pass through the first hydrophobic valve and the second hydrophobic valve to be stored in the filter, so that the temperature in the filter is increased. When the temperature value detected by the temperature detection element reaches the preset temperature, the high-temperature environment in the filter can sterilize the filter, and the timing also starts at the same time. When the timing reaches the preset time, the high-temperature environment in the filter has sufficiently sterilized the filter, and the filter sterilization is completed. When the filter sterilization is completed, the second control valve is closed, the input of pure steam into the second control valve is stopped, the first control valve is opened, and the special gas is transported into the first control valve to blow and discharge the residual condensed water in the filter from the first hydrophobic valve and the second hydrophobic valve, so as to ensure the dryness of the filter sterilization module and avoid the breeding of microorganisms due to the residual condensed water in the filter sterilization module, thereby improving the reliability and stability of the filter sterilization module and the cell culture system. When the filter needs to filter the special gas, the second control valve, the third control valve and the fifth control valve are closed, the first control valve and the fourth control valve are opened, and the special gas is transported into the first control valve, so that the special gas can pass through the filter to be filtered, thereby improving the applicability of the filter sterilization module and the cell culture system. Compared with the traditional filter sterilization method, the filter sterilization module in the present application can sterilize the filter without dismounting the filter from the cell culture system, thereby avoiding the risk of secondary pollution during the sterilization, transfer and installation of the traditional filter, and avoiding damage caused by bumping during the dismounting, cleaning and sterilization of the transfer process, thereby improving the reliability and service life of the filter sterilization module and the cell culture system. Moreover, the filter sterilization module in the present application has the functions of online sterilization and blowing and discharging the residual condensed water after sterilization, thereby ensuring the cleanliness of the filtered special gas and the dryness of the filter sterilization module, and improving the applicability and reliability of the filter sterilization module and the cell culture system. In addition, the filter sterilization module and the cell culture system also have the advantages of compact structure, small space occupation, reduced manual dismounting workload, convenient transportation and installation, and convenient operation and maintenance. BRIEF DESCRIPTION OF DRAWINGS

[0021] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application, and are incorporated in and constitute a part of this application. The drawings illustrate embodiments of the present application and, together with the description, serve to explain the application.

[0022] In order to make the technical solutions in the embodiments of the present application clearer, the accompanying drawings needed in the embodiments will be briefly introduced. Obviously, the accompanying drawings in the following description only show some embodiments of the present application, and other drawings can be obtained by those skilled in the art without any creative effort.

[0023] Figure 1 A piping and instrument diagram of a filter sterilization module of an embodiment;

[0024] Figure 2 A structural diagram of a filter sterilization module of an embodiment; Figure 1

[0025] A flow chart of a filter sterilization method of an embodiment. Figure 3 Legend of reference signs:

[0026] 10, filter sterilization module; 100, filter; 110, first connection end; 120, second connection end; 130, third connection end; 200, temperature detection element; 300, first control valve; 400, second control valve; 500, third control valve; 600, fourth control valve; 700, first steam trap; 800, fifth control valve; 900, second steam trap; 1000, sixth control valve; 1100, seventh control valve; 1200, first straight pipe; 1300, second straight pipe; 1400, first pressure detection element; 1500, second pressure detection element; 1600, connecting pipe; 1700, third steam trap; 1800, one-way valve; 1900, drain pipe; 2000, pressure reducing valve.

[0027] DETAILED DESCRIPTION

[0028] In order to make the above-mentioned objects, features and advantages of the present application more apparent and understandable, the specific embodiments of the present application will be described in detail below with reference to the accompanying drawings. In the following description, a lot of specific details are set forth in order to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the spirit of the present application, so the present application is not limited to the specific embodiments disclosed below.

[0029] In an embodiment, a cell culture system is provided, which includes a filter sterilization module 10. In this way, the filter sterilization module 10 can filter the special gas input into the cell culture system, so as to keep the special gas in the cell culture system clean, thereby improving the reliability of the cell culture system.

[0030] The special gas refers to the special gas at normal pressure in cell culture, such as carbon dioxide, oxygen, nitrogen, etc. ​

[0031] In this embodiment, the filter sterilization module 10 can be applied to cell culture systems, food processing systems, or other systems that require sterilization of the filter 100. This application uses the application of the filter sterilization module 10 in a cell culture system as an example for illustration and should not be construed as limiting this application.

[0032] like Figure 1 and Figure 2 As shown, in one embodiment, a filter sterilization module 10 is provided, including a filter 100 and a temperature detection element 200. The filter 100 is provided with a first connection terminal 110, a second connection terminal 120 and a third connection terminal 130 that are interconnected. The first connection terminal 110 is connected to the first control valve 300 and the second control valve 400. The second connection terminal 120 is connected to the input terminal of the third control valve 500 and the fourth control valve 600. The output terminal of the third control valve 500 is connected to the first drain valve 700. The third connection terminal 130 is connected to the fifth control valve 800 and the second drain valve 900 in sequence. The temperature detection element 200 is used to detect the temperature inside the filter 100.

[0033] When the filter 100 needs to be sterilized, the first control valve 300 and the fourth control valve 600 are closed, the second control valve 400, the third control valve 500 and the fifth control valve 800 are opened, and pure steam is input into the second control valve 400, so that the non-condensable gas and the condensed water in the filter 100 can be purged, and then the non-condensable gas and the condensed water in the filter 100 can be discharged through the first hydrophobic valve 700 and the second hydrophobic valve 900, while the pure steam in the filter 100 cannot pass through the first hydrophobic valve 700 and the second hydrophobic valve 900 to be stored in the filter 100, so that the temperature in the filter 100 is increased. When the temperature value detected by the temperature detection element 200 reaches the preset temperature, the high-temperature environment in the filter 100 can sterilize the filter 100, and the timing also starts at the same time. When the timing reaches the preset time, the high-temperature environment in the filter 100 has sufficiently sterilized the filter 100, and the sterilization of the filter 100 is completed. When the sterilization of the filter 100 is completed, the second control valve 400 is closed, the input of pure steam into the second control valve 400 is stopped, the first control valve 300 is opened, and special gas is transported into the first control valve 300 to purge the residual condensed water in the filter 100 and discharge it from the first hydrophobic valve 700 and the second hydrophobic valve 900, so as to ensure the dryness of the filter sterilization module 10 and avoid the breeding of microorganisms due to the residual condensed water in the filter sterilization module 10, thereby improving the reliability and stability of the filter sterilization module 10. When the filter 100 needs to filter special gas, the second control valve 400, the third control valve 500 and the fifth control valve 800 are closed, the first control valve 300 and the fourth control valve 600 are opened, and special gas is transported into the first control valve 300, so that the special gas can pass through the filter 100 to be filtered, thereby improving the applicability of the filter sterilization module 10. Compared with the traditional filter sterilization method, the filter sterilization module 10 in the present application can sterilize the filter 100 without dismounting the filter 100 from the cell culture system, thereby avoiding the risk of secondary pollution during the sterilization, transfer and installation of the traditional filter, and avoiding damage caused by bumping during the dismounting, cleaning and sterilization of the transfer process, thereby improving the reliability and service life of the filter sterilization module 10. Moreover, the filter sterilization module 10 in the present application has the functions of online sterilization and purging of residual condensed water after sterilization, thereby ensuring the cleanliness of the filtered special gas and the dryness of the filter sterilization module 10, and improving the applicability and reliability of the filter sterilization module 10. In addition, the filter sterilization module 10 has the advantages of compact structure, small space occupation, reduced manual dismounting workload, convenient transportation and installation, and convenient operation and maintenance.

[0034] The pure steam refers to steam meeting the requirements of the pure steam item in the Chinese Pharmacopoeia.

[0035] The size of the filter 100 can be flexibly adjusted according to actual needs.

[0036] The temperature detection element 200 can be a thermometer, a temperature sensor, or other temperature detection device. The temperature detection element 200 can be arranged at the first connecting end 110, the second connecting end 120, the third connecting end 130, or other positions. In the embodiment, the temperature detection element 200 is arranged at the output end of the third control valve 500. In this way, along the flow direction of the pure steam, the temperature of the pure steam gradually decreases, and when the output end of the third control valve 500 reaches the preset temperature, the temperature in the filter sterilization module 10 is greater than or equal to the preset temperature, ensuring that the filter 100 can be sterilized, and the reliability of the filter sterilization module 10 is improved.

[0037] When the temperature value detected by the temperature detection element 200 reaches the preset temperature, pure steam is continuously input into the second control valve 400, so that the temperature in the filter 100 is above the preset temperature and continuously maintained for a preset time, to ensure that the filter 100 is sufficiently sterilized.

[0038] The value of the preset temperature can be flexibly adjusted according to actual needs. For example, the value of the preset temperature is greater than or equal to 121°C. The preset time can be flexibly adjusted according to actual needs. For example, the value of the preset time is greater than or equal to 20 min.

[0039] As shown in FIGS. 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10, the filter sterilization module 10 further comprises a temperature detection element 200. Figure 1 Figure 2 As shown in FIGS. 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10, the filter sterilization module 10 further comprises a sixth control valve 1000, which is in communication with the output end of the third control valve 500. In this way, when pure steam is input into the second control valve 400, the sixth control valve 1000 is also opened synchronously, so that the non-condensable gas and condensed water in the filter 100 can be quickly discharged from the sixth control valve 1000, improving the sterilization efficiency of the filter sterilization module 10. When the non-condensable gas and condensed water in the filter 100 are completely discharged, the sixth control valve 1000 is closed, ensuring that the pure steam can be stored in the filter 100, so that the temperature in the filter 100 can be quickly increased to the preset temperature, improving the sterilization efficiency of the filter sterilization module 10.

[0040] Whether the non-condensable gas and condensed water in the filter 100 are completely discharged can be determined by the time of pure steam purging, that is, if the pure steam purges the filter 100 for a specific time, it is determined that the non-condensable gas and condensed water in the filter 100 are completely discharged; otherwise, it is determined that the non-condensable gas and condensed water in the filter 100 are not completely discharged.

[0041] As shown in FIGS. 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10, the filter sterilization module 10 further comprises a temperature detection element 200. Figure 1 ​and Figure 2 As shown, in one embodiment, the filter sterilization module 10 further includes a seventh control valve 1100, the input end of which is connected to the first connection end 110. Thus, when pure steam is input into the second control valve 400, the condensate at the first connection end 110 of the filter 100 can be discharged through the seventh control valve 1100, allowing the temperature inside the filter 100 to rise rapidly to the preset temperature (avoiding the condensate from absorbing heat and evaporating, which would affect the heating rate inside the filter 100), thereby improving the sterilization efficiency of the filter sterilization module 10.

[0042] like Figure 1 and Figure 2 As shown, furthermore, the output end of the seventh control valve 1100 is connected to the input end of the second steam trap 900. Thus, condensate at the first connection end 110 can be discharged through the seventh control valve 1100 and the second steam trap 900, while pure steam at the first connection end 110 cannot be discharged through the second steam trap 900. This ensures that all pure steam input from the second control valve 400 can be delivered into the filter 100, improving the purging effect of the pure steam purging filter 100.

[0043] like Figure 1 and Figure 2 As shown, optionally, the filter sterilization module 10 further includes an inclined first straight pipe 1200. The output end of the first straight pipe 1200 is located above the input end of the first straight pipe 1200. The output end of the first straight pipe 1200 is connected to the first connection end 110, and the input end of the first straight pipe 1200 is connected to the output ends of the first control valve 300, the second control valve 400, and the seventh control valve 1100. In this way, the first straight pipe 1200 can guide the condensate at the first connection end 110, allowing the condensate at the first connection end 110 and the condensate in the first straight pipe 1200 to flow to the input end of the seventh control valve 1100 and be discharged through the seventh control valve 1100 and the second drain valve 900, thus improving the reliability of the filter sterilization module 10. Furthermore, the condensate in the first control valve 300 and the second control valve 400 can also be discharged through the seventh control valve 1100 and the second drain valve 900, further improving the reliability of the filter sterilization module 10.

[0044] The output end of the first straight pipe 1200 can be directly connected to the first connection end 110, the input end of the first straight pipe 1200 can be directly connected to the output end of the first control valve 300, the input end of the first straight pipe 1200 can be directly connected to the output end of the second control valve 400, and the input end of the first straight pipe 1200 can be directly connected to the input end of the seventh control valve 1100. Alternatively, they can be connected via pipelines, as long as the condensate at the first connection end 110 can be discharged through the seventh control valve 1100 and the second steam trap 900, while the pure steam at the first connection end 110 cannot be discharged through the second steam trap 900.

[0045] The tilt angle of the first straight pipe 1200 can be flexibly adjusted according to actual usage needs. Specifically, in this embodiment, the tilt slope of the first straight pipe 1200 is 1% (that is, the ratio of the height of the first straight pipe 1200 in the vertical direction to the width of the first straight pipe 1200 in the horizontal direction is 1:100).

[0046] like Figure 1 and Figure 2 As shown, optionally, the filter sterilization module 10 further includes an inclined second straight pipe 1300. The input end of the second straight pipe 1300 is located above the output end of the second straight pipe 1300. The input end of the second straight pipe 1300 is connected to the third connection end 130, and the output end of the second straight pipe 1300 is connected to the input end of the second steam trap 900. In this way, the condensate in the second straight pipe 1300 can flow to the input end of the second steam trap 900 and be discharged through the second steam trap 900, thereby improving the reliability of the filter sterilization module 10.

[0047] The inlet and outlet of the second straight pipe 1300 can be directly connected to the third connection end 130, and the outlet of the second straight pipe 1300 can be directly connected to the inlet of the second steam trap 900. Alternatively, they can be connected via a pipeline, as long as the condensate in the second straight pipe 1300 can be discharged through the second steam trap 900.

[0048] The inclination slope of the second straight pipe 1300 can be flexibly adjusted according to actual usage needs. Specifically, in this embodiment, the inclination slope of the second straight pipe 1300 is 1% (that is, the ratio of the height of the second straight pipe 1300 in the vertical direction to the width of the second straight pipe 1300 in the horizontal direction is 1:100).

[0049] like Figure 1 and Figure 2As shown, in one embodiment, the first connection end 110 is provided with a first pressure detection element 1400, which is used to detect the pressure at the first connection end 110. The second connection end 120 is provided with a second pressure detection element 1500, which is used to detect the pressure at the second connection end 120. Thus, during the filtration of the special gas by the filter 100, the working state of the filter 100 can be determined based on the pressure difference between the first pressure detection element 1400 and the second pressure detection element 1500. That is, when the pressure difference between the first pressure detection element 1400 and the second pressure detection element 1500 is zero or less than a preset pressure difference, the filter 100 is working normally; when the pressure difference between the first pressure detection element 1400 and the second pressure detection element 1500 is greater than or equal to the preset pressure difference, the filter 100 is malfunctioning. This allows operators to promptly detect malfunctions in the filter 100 and take corresponding measures, improving the safety of the filter sterilization module 10.

[0050] Both the first pressure sensing element 1400 and the second pressure sensing element 1500 can be pressure gauges, pressure sensors, or other pressure sensing devices. The preset pressure difference value can be flexibly adjusted according to actual usage needs.

[0051] like Figure 1 and Figure 2 As shown, optionally, the filter sterilization module 10 also includes a pressure reducing valve 2000, which is connected to the input terminal of the first control valve 300. Thus, the pressure reducing valve 2000 can adjust the pressure of the special gas input to the first connection terminal 110, improving the applicability of the filter sterilization module 10.

[0052] like Figure 1 and Figure 2 As shown, in one embodiment, the filter 100 sterilization system further includes a connecting pipe 1600 and a third steam trap 1700. The input end of the second control valve 400 is connected to the connecting pipe 1600, and the output end of the connecting pipe 1600 is connected to the third steam trap 1700. Thus, before pure steam is input to the second control valve 400, the condensate in the pure steam can be discharged through the connecting pipe 1600 and the third steam trap 1700, reducing the amount of condensate delivered to the filter 100 and ensuring that the temperature inside the filter 100 can quickly rise to the preset temperature, thereby improving the sterilization efficiency of the filter sterilization module 10.

[0053] like Figure 1 and Figure 2As shown, further, the filter sterilization module 10 further comprises a one-way valve 1800, which is in communication with the output end of the third drain valve 1700. In this way, the one-way valve 1800 can prevent the condensed water from flowing back into the connecting pipe 1600, thereby improving the reliability of the filter sterilization module 10.

[0054] Optionally, the filter sterilization module 10 further comprises a first manual valve, and the connecting pipe 1600 and the second control valve 400 are in communication through the first manual valve. In this way, when the filter sterilization module 10 is maintained or repaired, the first manual valve is closed to prevent the high-temperature pure steam from being delivered from the second control valve 400 to the filter 100 to scald the operator, thereby improving the safety of the filter sterilization module 10.

[0055] Optionally, the filter sterilization module 10 further comprises a second manual valve and a third pressure detection element, the input end of the second manual valve is in communication with the input end of the connecting pipe 1600, and the output end of the second manual valve is in communication with the third pressure detection element. In this way, the operator can know the pressure of the pure steam input into the filter sterilization module 10.

[0056] Optionally, the filter sterilization module 10 further comprises a third manual valve, and the input end of the third manual valve is in communication with the input end of the connecting pipe 1600. In this way, the operator can sample the pure steam input into the filter sterilization module 10 from the output end of the third manual valve, thereby improving the reliability of the filter sterilization module 10.

[0057] As shown in Figure 1 and Figure 2 In one embodiment, the filter sterilization module 10 further comprises a drain pipe 1900, which is in communication with the output end of the one-way valve 1800, the output end of the second drain valve 900, the output end of the sixth control valve 1000, and the output end of the first drain valve 700. In this way, the filter sterilization module 10 can concentrate the condensed water through the drain pipe 1900, thereby improving the convenience of the filter sterilization module 10.

[0058] As shown in Figure 1 and Figure 2 In one embodiment, the filter sterilization module 10 further comprises a controller, which is in communication with the temperature detection element 200. In this way, the controller can actually monitor the sterilization of the filter 100 through the temperature detection element 200, and record the sterilization process of the filter 100, thereby facilitating the operator to track the sterilization of the filter 100, and improving the applicability of the filter sterilization module 10.

[0059] The controller can be a single-chip microcomputer, a programmable logic controller or other control element. The controller is in communication connection with the temperature detection element 200, and can be connected through a data line, Bluetooth, a wireless communication network or other communication means.

[0060] Optionally, the controller is in communication connection with the first pressure detection element 1400 and the second pressure detection element 1500. In this way, the intelligent degree of the filter sterilization module 10 is improved.

[0061] In the embodiment, the first control valve 300, the second control valve 400, the third control valve 500, the fourth control valve 600, the fifth control valve 800, the sixth control valve 1000 and the seventh control valve 1100 can all be pneumatic ball valves. In this way, the convenience of the filter sterilization module 10 is improved.

[0062] As shown in FIG. 1, in one embodiment, a filter 100 sterilization method is provided, comprising the following steps: Figure 3

[0063] S100, the first control valve 300 and the fourth control valve 600 are closed, the second control valve 400, the third control valve 500 and the fifth control valve 800 are opened, and pure steam is input into the second control valve 400 to discharge the non-condensable gas and condensed water in the filter 100, and the temperature in the filter 100 is increased. In this way, by discharging the non-condensable gas and condensed water in the filter 100, the temperature in the filter 100 can be quickly raised, and the sterilization efficiency of the filter sterilization module 10 is improved.

[0064] S200, when the temperature value detected by the temperature detection element 200 reaches the preset temperature, timing is started. In this way, the temperature in the filter 100 can be maintained above the preset temperature, so that the filter 100 can be continuously sterilized, and the reliability of the filter sterilization module 10 is improved.

[0065] S300, when the timing reaches the preset time, the filter 100 sterilization is completed. In this way, the temperature in the filter 100 is above the preset temperature and continuously maintained for the preset time, so as to ensure that the filter 100 is sufficiently sterilized, and the reliability of the filter sterilization module 10 is improved.

[0066] ​S400, the second control valve 400 is closed, the pure steam input into the second control valve 400 is stopped, the first control valve 300 is opened, and the special gas is delivered into the first control valve 300, so that the residual condensed water in the filter 100 is purged. In this way, the residual condensed water in the filter 100 can be discharged from the first and second traps 700 and 900, the dryness in the filter sterilization module 10 is ensured, the breeding of microorganisms in the filter sterilization module 10 due to the residual condensed water is avoided, and the reliability and stability of the filter sterilization module 10 are improved.

[0067] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. The term "and / or" used in the present application includes any and all combinations of one or more related listed items.

[0068] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, for example, two, three, etc., unless otherwise explicitly specified and limited.

[0069] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or can be integrated; can be mechanically connected, or can be electrically connected; can be directly connected, or can be indirectly connected through an intermediate medium; can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0070] In the present application, unless specifically stated and limited otherwise, a first feature "on" or "under" a second feature can be direct contact between the first and second features, or indirect contact between the first and second features through an intervening medium. Also, a first feature "over", "above" and "on top of" a second feature can be directly above or obliquely above the second feature, or simply mean that the first feature is horizontally higher than the second feature. A first feature "under", "below" and "underneath" a second feature can be directly below or obliquely below the second feature, or simply mean that the first feature is horizontally lower than the second feature.

[0071] It is noted that when an element is referred to as being "on" or "connected to" another element, it can be directly on or connected to the other element or intervening elements can also be present. In the description of the present application, the terms "vertical", "horizontal", "up", "down", "left", "right", and similar expressions are used for the purpose of explanation only and not as a limitation.

[0072] It should also be understood that, in interpreting the connection relationship or position relationship of the elements, although not explicitly described, the connection relationship and position relationship are interpreted to include an error range acceptable by the specific value determined by the person skilled in the art. For example, "about", "approximately" or "substantially" can mean within one or more standard deviations, without limitation.

[0073] The technical features of the above embodiments can be combined in any manner. In order to make the description concise, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combination of the technical features does not exist contradictory, it should be considered as the scope of the present application.

[0074] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation to the scope of the patent. It should be pointed out that for those skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the scope of the present application. Therefore, the scope of protection of the present application should be subject to the appended claims.

Claims

1. A filter sterilization method applied to a filter sterilization module, characterized by, The filter sterilization module comprises a filter and a temperature detection element, the filter is provided with a first connecting end, a second connecting end and a third connecting end which are communicated with each other, the first connecting end is communicated with a first control valve and a second control valve, the second connecting end is communicated with an input end of a third control valve and a fourth control valve, an output end of the third control valve is communicated with a first steam trap, the third connecting end is communicated with a fifth control valve and a second steam trap in sequence, and the temperature detection element is used for detecting the temperature in the filter; the filter sterilization module further comprises a sixth control valve and a seventh control valve, the sixth control valve is communicated with the output end of the third control valve, and an input end of the seventh control valve is communicated with the first connecting end; the filter sterilization method comprises: closing the first control valve and the fourth control valve, opening the second control valve, the third control valve and the fifth control valve, and inputting pure steam into the second control valve to discharge non-condensable gas and condensed water in the filter, and the temperature in the filter is increased; when the temperature value detected by the temperature detection element reaches a preset temperature, timing is started; when the timing reaches a preset time, the filter sterilization is completed; closing the second control valve, stopping inputting pure steam into the second control valve, opening the first control valve, and conveying special gas into the first control valve to purge the residual condensed water in the filter.

2. A filter sterilization module for implementing the filter sterilization method as claimed in claim 1, characterized in that, An output end of the seventh control valve is communicated with an input end of the second steam trap.

3. The filter sterilization module of claim 2, wherein, The filter sterilization module further comprises an obliquely arranged first straight pipe, an output end of the first straight pipe is located above an input end of the first straight pipe, the output end of the first straight pipe is communicated with the first connecting end, and the input end of the first straight pipe is communicated with an output end of the first control valve, an output end of the second control valve and an input end of the seventh control valve.

4. The filter sterilization module of claim 2 or 3, wherein, The filter sterilization module further comprises an obliquely arranged second straight pipe, an input end of the second straight pipe is located above an output end of the second straight pipe, the input end of the second straight pipe is communicated with the third connecting end, and the output end of the second straight pipe is communicated with the input end of the second steam trap.

5. The filter sterilization module of claim 2 or 3, wherein, The first connecting end is provided with a first pressure detection element, the first pressure detection element is used for detecting the pressure at the first connecting end, the second connecting end is provided with a second pressure detection element, and the second pressure detection element is used for detecting the pressure at the second connecting end.

6. A cell culture system, characterized by The filter sterilization module comprises the filter sterilization module according to any one of claims 2 to 5.

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