A sampling device for a constant-temperature incubator for edible fungi and a method of use

Abstract

A kind of edible fungus thermostatic incubator sampling device and method, belong to mould fixed limiting field. Including outer cylinder, connecting pipe, inner cylinder, rubber plug, control device and power device;The lower end of the inner cylinder is fixedly connected with rubber plug, the upper end of the inner cylinder is provided with power device, and the inner cylinder, rubber plug are arranged in the inside of outer cylinder. The lateral bottom end of the outer cylinder is equipped with through hole I, and the lateral bottom end of the inner cylinder is equipped with through hole II. One end of the connecting pipe is fixedly connected on the outside of outer cylinder, and the other end of the connecting pipe is fixedly connected on the outside of inner cylinder, and through hole I and through hole II are communicated with connecting pipe. The bottom end of the outer cylinder is equipped with liquid inlet. The control device is arranged in liquid inlet. The present application can not only ensure that the sample is not contaminated during the process of being divided into multiple parts, but also store multiple samples in the inner cylinder, save the space occupied when carrying samples, and easy to carry.

Description

Technical Field

[0001] This invention relates to a sampling device and method for use in a constant temperature incubator for edible fungi, belonging to the field of mold fixing and limiting. Background Technology

[0002] Patent number CN208223859U, entitled "A Utility Model Patent for Sampling Device for Edible Fungus Culture Media," describes a device that, while allowing users to hold a handle to secure a bowl-shaped rubber sleeve over the culture medium and tighten it by pulling a ring to prevent contamination during sampling, and then rotating the handle to lower the handle and sampling tube into the medium, followed by rotating the drive lever to raise the piston block for sampling, creates a relatively enclosed environment that prevents contamination and improves accuracy. However, this device often requires dividing the sample into multiple portions for different tests. The patent does not allow for direct sample division, making the division process susceptible to contamination. Therefore, improvements are necessary. Summary of the Invention

[0003] The purpose of this invention is to solve the above-mentioned problems existing in the background art by providing a sampling device and method for using a constant temperature incubator for edible fungi.

[0004] The present invention achieves the above objectives by adopting the following technical solution:

[0005] A sampling device for a constant temperature incubator for edible fungi includes an outer cylinder, a connecting tube, an inner cylinder, a rubber stopper, a control device, and a power device. The lower end of the inner cylinder is fixedly connected to the rubber stopper, and the upper end of the inner cylinder is equipped with the power device. The inner cylinder and the rubber stopper are disposed inside the outer cylinder. The bottom side of the outer cylinder has a through hole I, and the bottom side of the inner cylinder has a through hole II. One end of the connecting tube is fixedly connected to the outside of the outer cylinder, and the other end of the connecting tube is fixedly connected to the outside of the inner cylinder. Both through holes I and II communicate with the connecting tube. The bottom end of the outer cylinder has a liquid inlet. The control device is disposed inside the liquid inlet.

[0006] A method for using a sampling device for an edible fungi constant temperature incubator, the method comprising the following steps:

[0007] Step 1: Pull the push plate to draw the culture medium into the outer cylinder;

[0008] Step 2: Push the push plate to move the rubber stopper and push the culture medium into the inner cylinder;

[0009] Step 3: Pull the pull ring upwards to seal the culture medium at the bottom of the horizontal plate.

[0010] Compared with the prior art, the beneficial effects of the present invention are: the present invention can not only ensure that the sample is not contaminated during the process of dividing it into multiple parts after sampling, but also that multiple samples are stored in the inner cylinder, saving space when carrying the sample and making it easy to carry. Attached Figure Description

[0011] Figure 1 This is a front view of a sampling device for an edible fungus constant temperature incubator according to the present invention;

[0012] Figure 2 This is a front view of the outer cylinder of a sampling device for an edible fungus constant temperature incubator according to the present invention;

[0013] Figure 3 This is a front view of the inner cylinder of a sampling device for an edible fungus constant temperature incubator according to the present invention;

[0014] Figure 4 This is a schematic diagram of the structure of the cylinder of a sampling device for a constant temperature incubator for edible fungi according to the present invention;

[0015] Figure 5 This is a top view of the central rod of a sampling device for a constant temperature incubator for edible fungi according to the present invention;

[0016] Figure 6 yes Figure 3 A magnified structural diagram of A in the middle;

[0017] Figure 7 This is a schematic diagram of the rubber stopper of a sampling device for an edible fungus constant temperature incubator according to the present invention;

[0018] Figure 8 This is a schematic diagram of the power unit of a sampling device for an edible fungus constant temperature incubator according to the present invention;

[0019] Figure 9 This is a bottom view of the movable plate I of the sampling device for a constant temperature incubator for edible fungi according to the present invention. Detailed Implementation

[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0021] Specific implementation method one: as follows Figure 1-9As shown, this embodiment describes a sampling device for a constant temperature incubator for edible fungi, including an outer cylinder 1, a connecting pipe 2, an inner cylinder 3, a rubber stopper 4, a control device 5, and a power device 6; the lower end of the inner cylinder 3 is fixedly connected to the rubber stopper 4, and the upper end of the inner cylinder 3 is provided with the power device 6; the inner cylinder 3 and the rubber stopper 4 are disposed inside the outer cylinder 1; the bottom side of the outer cylinder 1 is provided with a through hole I 14, and the bottom side of the inner cylinder 3 is provided with a through hole II 314; one end of the connecting pipe 2 is fixedly connected to the outside of the outer cylinder 1, and the other end of the connecting pipe 2 is fixedly connected to the outside of the inner cylinder 3, and both the through hole I 14 and the through hole II 314 are connected to the connecting pipe 2; the bottom end of the outer cylinder 1 is provided with a liquid inlet 15; the control device 5 is disposed inside the liquid inlet 15.

[0022] Specific implementation method two: such as Figure 1-9 As shown, this embodiment is a further explanation of the first specific embodiment. The outer cylinder 1 includes a cylinder body 11. The inner wall of the cylinder body 11 is provided with a plurality of limiting grooves 12 parallel to its central axis. The outer wall of the inner cylinder 3 is fixedly connected with a slider that slides in cooperation with the limiting grooves 12 to restrict the relative rotation of the inner cylinder 3 and the outer cylinder 1, thereby preventing the connecting pipe 2 from getting tangled together.

[0023] Specific implementation method three: such as Figure 1-9 As shown, this embodiment is a further explanation of the first specific embodiment. The inner cylinder 3 includes a cylinder body 31. The upper part of the side of the cylinder body 31 is provided with a through hole II 314. The outer side of the cylinder body 31 is also provided with a sliding groove I 313 located below the through hole II 314. A sliding groove II 311 is provided between the sliding groove I 313 and the through hole II 314 to connect the two. An L-shaped sliding plate 312 is provided in the sliding groove I 313 and the sliding groove II 311 to slide with them. The horizontal end of the L-shaped sliding plate 312 is exposed on the outer side of the cylinder body 31.

[0024] Specific implementation method four: such as Figure 1-9 As shown, this embodiment is a further explanation of the first specific embodiment. The cylinder 31 has a cavity inside. A central rod 34 is fixedly connected to the inner wall of the cavity by a partition 37. Multiple partitions 37 are provided to divide the cavity into multiple storage chambers, and each storage chamber is connected to the corresponding through hole II 314.

[0025] Specific implementation method five: such as Figure 1-9As shown, this embodiment is a further explanation of specific embodiment one. The control device 5 includes a slide rod 51, a baffle I 52, a connecting rod 53, and a baffle II 56. The bottom end of the central rod 34 is provided with a slide rail I 35, which slides in cooperation with the slide rod 51. The bottom end of the slide rod 51 is fixedly connected to the baffle I 52, and the lower end of the baffle I 52 is fixedly connected to the connecting rod 53. The middle and lower part of the connecting rod 53 is fixedly connected to the baffle II 56. The baffle I 52 is located inside the outer cylinder 1, the connecting rod 53 passes through the liquid inlet 15, and the baffle II 56 is located below the liquid inlet 15. The bottom of the connecting rod 53 is provided with a liquid inlet chamber 55, and the middle position of the side of the connecting rod 53 is provided with a connecting hole 54, so that the liquid inlet chamber 55 communicates with the liquid inlet 15. The bottom end of the inner cavity of the outer cylinder 1 is provided with a groove 13, and the baffle I 52 slides in cooperation with the groove 13.

[0026] Specific implementation method six: such as Figure 1-9 As shown, this embodiment is a further explanation of the first specific embodiment. The diameters of the baffle I 52 and the baffle II 56 are larger than the inner diameter of the liquid inlet 15, and the diameter of the connecting rod 53 is smaller than the inner diameter of the liquid inlet 15.

[0027] Specific implementation method seven: such as Figure 1-9 As shown, this embodiment is a further explanation of the first specific embodiment, and there is friction between the slide rod 51 and the inner wall of the slide rail I 35 of the center rod 34.

[0028] Specific implementation method eight: such as Figure 1-9 As shown, this embodiment is a further explanation of specific embodiment one. The rubber stopper 4 includes an annular rubber plate I 41, an inner rubber ring 42, an annular rubber plate II 43, an outer rubber ring 44, and multiple movable blocks 45. The annular rubber plate I 41 and the annular rubber plate II 43 are fixedly connected by the inner rubber ring 42 and the outer rubber ring 44, and the annular rubber plate I 41 is fixedly connected to the lower end of the cylinder 31. The sliding rod 51 passes through the inner rubber ring 42. The side of the outer rubber ring 44 is provided with multiple perforations, and each perforation contains a movable block 45 that slides with the perforation. The movable block 45 slides with the corresponding limiting groove 12. The friction between the annular rubber plate II 43 and the inner wall of the outer cylinder 1 is greater than the friction between the annular rubber plate I 41 and the inner wall of the outer cylinder 1.

[0029] Specific implementation method nine: as follows Figure 1-9As shown, this embodiment is a further explanation of specific embodiment one. The power device 6 includes a pull ring 61, a push plate 62, a movable plate I 63, an annular plate 64, a vertical rod 66, a support plate 67, a spring 68, a movable plate II 69, and a limiting rod 610. The upper end of the central rod 34 is provided with a limiting groove 36, and the limiting rod 610 slides in conjunction with the limiting groove 36. The upper end of the limiting rod 610 passes through the push plate 62 and is fixedly connected to the movable plate I 63. The upper end of the push plate 62 is provided with a circular groove, and the movable plate I 63 slides in conjunction with the circular groove. The bottom surface of the circular groove of the push plate 62 is also provided with multiple holes 65. The lower end of the movable plate I 63 is provided with an annular groove, and the annular plate 64 passes through... The bearing is connected in the annular groove; one end of the vertical rod 66 is fixedly connected to the lower end of the annular plate 64, and the other end of the vertical rod 66 passes through the corresponding hole 65 and is fixedly connected to the support plate 67; the upper end of the support plate 67 is fixedly connected to the spring 68, and the upper end of the spring 68 is fixedly connected to the movable plate II 69 sleeved on the corresponding vertical rod 66; the spring force of the spring 68 on each vertical rod 66 is different; a horizontal plate 32 is fixedly connected between two adjacent partition plates 37, the horizontal plate 32 is located below the through hole II 314 and above the movable plate II 69, and the movable plate II 69 is provided with a circular hole 33, the cross-sectional area of ​​the movable plate II 69 is larger than the cross-sectional area of ​​the circular hole 33.

[0030] Specific implementation method ten: such as Figure 1-9 As shown in the figure, this embodiment describes a method for using a sampling device for an edible fungus constant temperature incubator, the method comprising the following steps:

[0031] Step 1: Pull the push plate 62 to draw the culture medium into the outer cylinder 1;

[0032] Step 2: Push the push plate 62 to move the rubber stopper 4 and push the culture medium into the inner cylinder 3;

[0033] Step 3: Pull the pull ring 61 upwards to seal the culture medium at the lower end of the horizontal plate 32.

[0034] The working principle of this invention is as follows: When using this device to extract culture medium, the inlet 15 is placed into the culture medium, and then the push plate 62 is pulled. The push plate 62 is fixedly connected to the top of the central rod 34. Therefore, the push plate 62 drives the central rod 34 to move upward. The central rod 34 drives the partition plate 37 and the rubber stopper 4 to move upward. During the upward movement of the central rod 34, due to the friction between the slide rail I 24 at its lower end and the slide rod 51, the central rod 34 drives the slide rod 51 and thus drives the control device 5 to move upward until the baffle II 56 contacts the inlet 15. After that, the control device 5 and the outer cylinder 1 are relatively stationary. The culture medium enters the interior of the outer cylinder 1 from the inlet cavity 55 and the connecting hole 54. Then, the pull ring 61 is pulled upward to move the movable plate I 66. 3. Move upward to disengage from push plate 62, then push push plate 62 downward. Push plate 62 drives center rod 34 downward, which in turn drives slide rod 51 downward through friction. When baffle I 52 moves into tank 13, baffle I 52 blocks liquid inlet 15. The culture medium inside outer cylinder 1 enters the interior of cylinder 31 through connecting pipe 2 and through hole II 314 as rubber stopper 4 moves downward. After passing through round hole 33, it moves to the bottom of horizontal plate 32. Finally, push plate 62 is pulled upward again so that all movable plates II 69 are close to the lower end face of horizontal plate 32, sealing round hole 33. It can then be carried. In this process, the culture medium can be prevented from being contaminated and can be divided into multiple portions for storage, which is convenient for subsequent different tests.

[0035] As the pusher plate 62 moves the inner cylinder 3 downwards, when the rubber stopper 4 contacts the bottom of the outer cylinder 1, the upper end face of the outer cylinder 1 pushes the L-shaped sliding plate 312 upwards, sealing the through hole II 314 and preventing the culture medium from flowing back into the connecting pipe 2 when it is poured in. At the same time, as the pusher plate 62 moves downwards, because the friction between the annular rubber plate II 43 and the inner wall of the outer cylinder 1 is greater than the friction between the annular rubber plate I 41 and the inner wall of the outer cylinder 1, and there is no friction between the annular rubber plate I 41 and the inner wall of the outer cylinder 1, the distance between the annular rubber plate II 43 and the annular rubber plate I 41 shortens as the rubber stopper 4 moves downwards, compressing the inner rubber ring 42. The rubber outer ring 44 increases the internal air pressure of the space enclosed by the annular rubber plate II 43, annular rubber plate I 41, rubber inner ring 42, and rubber outer ring 44, thereby pushing the movable block 45 to move away from the rubber outer ring 44 and into the corresponding limiting groove 12. During the downward movement of the rubber stopper 4, a cleaning pad is adhered to the side wall of the movable block 45 near the inner wall of the outer cylinder 1. The cleaning pad cleans the limiting groove 12 and reduces the culture medium residue in the limiting groove 12. The side of the movable block 45 near the outer cylinder 1 is set as an inclined surface. After contacting the bottom of the limiting groove 12, the movable block 45 can be pushed back into the rubber outer ring 44 by squeezing.

[0036] When it is necessary to pour out the culture medium inside the inner cylinder 3, push the movable plate I 63 towards the push plate 62. Due to the different elastic forces of the springs 38, the movable plate II 69 will disengage from the horizontal plate 32 in turn during the process of pushing the movable plate I 63. Therefore, when pouring out the culture medium, push the movable plate I 63 to make one of the movable plates II 69 disengage from the horizontal plate 32. Then, invert the present invention to pour out one portion of the culture medium. Repeat the above operation to pour out multiple portions of the culture medium in turn.

Claims

1. A sampling device for a constant temperature incubator for edible fungi, characterized in that: The device includes an outer cylinder (1), a connecting pipe (2), an inner cylinder (3), a rubber stopper (4), a control device (5), and a power device (6); the lower end of the inner cylinder (3) is fixedly connected to the rubber stopper (4), and the upper end of the inner cylinder (3) is provided with the power device (6). The inner cylinder (3) and the rubber stopper (4) are located inside the outer cylinder (1); the bottom side of the outer cylinder (1) is provided with a through hole I (14); one end of the connecting pipe (2) is fixedly connected to the outside of the outer cylinder (1), and the other end of the connecting pipe (2) is fixedly connected to the outside of the inner cylinder (3). The through hole I (14) and the through hole II (314) are both connected to the connecting pipe (2); the bottom end of the outer cylinder (1) is provided with a liquid inlet (15); the control device (5) is located inside the liquid inlet (15). The inner cylinder (3) includes an inner cylinder body (31); the upper side of the inner cylinder body (31) is provided with a through hole II (314), and the outer side of the inner cylinder body (31) is also provided with a sliding groove I (313) located below the through hole II (314), and a sliding groove II (311) connecting the two is provided between the sliding groove I (313) and the through hole II (314); the sliding groove I (313) and the sliding groove II (311) are provided with an L-shaped sliding plate (312) that slides with them, and the horizontal end of the L-shaped sliding plate (312) is exposed on the outer side of the inner cylinder body (31); The inner cylinder (31) has a cavity inside. A central rod (34) is fixedly connected to the inner wall of the cavity by a partition (37). Multiple partitions (37) are provided to divide the cavity into multiple storage cavities, and each storage cavity is connected to the corresponding through hole II (314). The power unit (6) includes a pull ring (61), a push plate (62), a movable plate I (63), an annular plate (64), a vertical rod (66), a support plate (67), a spring (68), a movable plate II (69), and a limiting rod (610). The upper end of the central rod (34) is provided with a central rod limiting groove (36), and the limiting rod (610) slides with the central rod limiting groove (36). The upper end of the limiting rod (610) passes through the push plate (62) and is fixedly connected to the movable plate I (63). The upper end of the push plate (62) is provided with a circular groove, and the movable plate I (63) slides with the circular groove. The bottom surface of the circular groove of the push plate (62) is also provided with multiple holes (65). The lower end of the movable plate I (63) is provided with an annular groove, and the annular plate (64) is connected in the annular groove by a bearing. One end of the vertical rod (66) is fixedly connected to the lower end of the annular plate (64), and the other end of the vertical rod (66) is fixedly connected to the support plate (67) through the corresponding hole (65); the upper end of the support plate (67) is fixedly connected to the spring (68), and the upper end of the spring (68) is fixedly connected to the movable plate II (69) sleeved on the corresponding vertical rod (66); the spring force of the spring (68) on each vertical rod (66) is different; a horizontal plate (32) is fixedly connected between two adjacent partitions (37), the horizontal plate (32) is located below the through hole II (314), and the horizontal plate (32) is located above the movable plate II (69), the movable plate II (69) is provided with a round hole (33), and the cross-sectional area of ​​the movable plate II (69) is larger than the cross-sectional area of ​​the round hole (33).

2. The sampling device for a constant temperature incubator for edible fungi according to claim 1, characterized in that: The outer cylinder (1) includes an outer cylinder body (11); the inner wall of the outer cylinder body (11) is provided with a plurality of cylinder limiting grooves (12) parallel to its central axis; the outer wall of the inner cylinder (3) is fixedly connected with a slider that slides in cooperation with the cylinder limiting grooves (12) to restrict the relative rotation of the inner cylinder (3) and the outer cylinder (1), thereby preventing the connecting pipe (2) from getting tangled together.

3. The sampling device for a constant temperature incubator for edible fungi according to claim 1, characterized in that: The control device (5) includes a slide rod (51), a baffle I (52), a connecting rod (53), and a baffle II (56); the bottom end of the center rod (34) is provided with a slide rail I (35), which slides in cooperation with the slide rod (51); the bottom end of the slide rod (51) is fixedly connected to a baffle I (52), and the lower end of the baffle I (52) is fixedly connected to a connecting rod (53); the middle and lower part of the connecting rod (53) is fixedly connected to a baffle II (56); the baffle II (56) is fixedly connected to the middle and lower part of the connecting rod (53); the baffle II (56) is fixedly connected to the center rod (54); the baffle II (54) is fixedly connected to the center rod (5 ... Plate I (52) is located inside the outer cylinder (1), connecting rod (53) passes through the liquid inlet (15), and baffle II (56) is located below the liquid inlet (15); the bottom of the connecting rod (53) is provided with a liquid inlet cavity (55), and the middle of the side of the connecting rod (53) is provided with a connecting hole (54) so ​​that the liquid inlet cavity (55) is connected to the liquid inlet (15); the bottom of the inner side of the outer cylinder (1) is provided with a groove (13), and baffle I (52) slides with the groove (13).

4. The sampling device for a constant temperature incubator for edible fungi according to claim 3, characterized in that: The diameters of the baffle I (52) and baffle II (56) are greater than the inner diameter of the inlet (15), and the diameter of the connecting rod (53) is smaller than the inner diameter of the inlet (15).

5. A sampling device for a constant temperature incubator for edible fungi according to claim 4, characterized in that: There is friction between the slide bar (51) and the inner wall of the slide rail I (35) of the center rod (34).

6. The sampling device for an edible fungi constant temperature incubator according to claim 3, characterized in that: The rubber stopper (4) includes an annular rubber plate I (41), an inner rubber ring (42), an annular rubber plate II (43), an outer rubber ring (44), and multiple movable blocks (45); the annular rubber plate I (41) and the annular rubber plate II (43) are fixedly connected by the inner rubber ring (42) and the outer rubber ring (44), and the annular rubber plate I (41) is fixedly connected to the lower end of the inner cylinder body (31); the sliding rod (51) passes through the inner rubber ring (42); the side of the outer rubber ring (44) is provided with multiple perforations, and each perforation contains a movable block (45) that slides with the perforation. The movable block (45) slides with the corresponding cylinder limiting groove (12); the friction between the annular rubber plate II (43) and the inner wall of the outer cylinder (1) is greater than the friction between the annular rubber plate I (41) and the inner wall of the outer cylinder (1).

7. The method of using the sampling device for a constant temperature incubator for edible fungi according to claim 6, characterized in that: The method of use includes the following steps: Step 1: Pull the push plate (62) to draw the culture medium into the outer cylinder (1); Step 2: Push the push plate (62) to drive the rubber stopper (4) and push the culture medium into the inner cylinder (3); Step 3: Pull the pull ring (61) upwards to seal the culture medium at the lower end of the horizontal plate (32).

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