A control method, device and storage medium for a laying medium

Abstract

The present application discloses a control method, device and storage medium for laying a medium. A suction control signal is received, and the sucker is controlled to suck the upper air column and the medium to be laid in sequence according to the suction control signal, wherein the volume of the upper air column is positively correlated with the viscosity of the medium to be laid and negatively correlated with the volume of the medium to be laid. A release control signal is then received, and the sucker is controlled to release the medium to be laid according to the release control signal. The method described in the present application sucks an air column into the sucker in advance, and when released, a certain pressure is provided by the air column to push out the medium to be laid, thereby controlling the generation of bubbles in the medium to be laid, so that the cell reproduction in the culture medium can be clearly observed when no bubbles are generated.

Description

Technical Field

[0001] The present application relates to the field of automation technology, and in particular to a control method, device and storage medium for laying a medium. Background Art

[0002] The soft agar colony formation assay, also known as the anchorage-independent growth assay, utilizes soft agar as a culture medium to grow cells in suspension. Because some cells can proliferate not only in an adherent state but also in suspension, monitoring their proliferation in soft agar can reflect their proliferation capacity, which is used in basic research on cell differentiation and clinical efficacy testing of tumor treatments. The specific process involves first preparing a lower agar layer and laying it on the bottom of the culture dish. Then, a top layer of culture medium containing cells is added to culture the cells.

[0003] In the prior art, for example, when laying the lower layer of agar, a discharge gun is generally used. During this process, bubbles are inevitably generated, which adds resistance to observing the cell growth in the culture medium. Therefore, how to reduce the bubbles generated during the release has become an urgent problem to be solved. Summary of the Invention

[0004] Based on the above problems, the present application provides a control method, device and storage medium for laying medium to reduce bubbles generated during release.

[0005] The present application discloses a method for controlling laying of a medium, the method comprising:

[0006] receiving an absorption control signal;

[0007] The suction device is controlled to sequentially suck the upper air column and the medium to be laid according to the suction control signal; the volume of the upper air column is positively correlated with the viscosity of the medium to be laid and negatively correlated with the volume of the medium to be laid;

[0008] receiving a release control signal;

[0009] The sucker is controlled to release the medium to be laid according to the release control signal.

[0010] Optionally, before receiving the absorption signal, the method further includes:

[0011] receiving an ambient temperature and a preset volume of the medium to be laid;

[0012] According to the ambient temperature and the preset volume, the volume of the upper air column is determined to be the first volume; and the viscosity of the medium to be laid is related to the ambient temperature.

[0013] Optionally, determining the volume of the upper air column as the first volume based on the ambient temperature and the preset volume includes:

[0014] When the ambient temperature is within a preset temperature range, the relationship between the first volume, the preset volume, and the ambient temperature is:

[0015] V1=-1 / 4*V-2*T+c;

[0016] Wherein, V1 represents the first volume, V represents the preset volume, and both are in μL; T represents the ambient temperature, and its unit is °C; and c is a constant parameter.

[0017] Optionally, the preset volume has a value range of less than 160 μL, the preset temperature range is 23° C. to 27° C., and the constant parameter has a value range of 95 to 120.

[0018] Optionally, after controlling the absorber to sequentially absorb the upper air column of the first volume and the medium to be laid according to the absorption control signal, the method further includes: controlling the absorber to also absorb the lower air column.

[0019] Optionally, the medium to be laid includes agar.

[0020] Optionally, the speed at which the absorber absorbs the upper air column, the medium to be laid and the lower air column is the same preset speed.

[0021] Optionally, the preset speed ranges from 70 μL / s to 100 μL / s.

[0022] Optionally, the volume of the lower air column ranges from 2 μL to 4 μL.

[0023] Based on the above-mentioned control method for laying a medium, the present application also discloses a control device for laying a medium, including: a suction signal receiving module, a suction control module, a release signal receiving module, and a release control module;

[0024] The suction signal receiving module is used to receive the suction control signal;

[0025] The suction control module is used to control the sucker to suck the upper air column and the medium to be laid in sequence according to the suction control signal; the volume of the upper air column is positively correlated with the viscosity of the medium to be laid, and negatively correlated with the volume of the medium to be laid;

[0026] The release signal receiving module is used to receive a release control signal;

[0027] The release control module is used to control the sucker to release the medium to be laid according to the release control signal.

[0028] Optionally, the device further comprises: a volume receiving module and a first volume determining module;

[0029] The volume receiving module is used to receive the ambient temperature and the preset volume of the medium to be laid;

[0030] The first volume determination module is used to determine the volume of the upper air column as a first volume according to the ambient temperature and the preset volume; the viscosity of the medium to be laid is related to the ambient temperature.

[0031] Optionally, the first volume determination module includes:

[0032] The relationship determination submodule is configured to determine that when the ambient temperature is within a preset temperature range, the relationship between the first volume, the preset volume, and the ambient temperature is:

[0033] V1=-1 / 4*V-2*T+c;

[0034] Wherein, V1 represents the first volume, V represents the preset volume, and both are in μL; T represents the ambient temperature, and its unit is °C; and c is a constant parameter.

[0035] Optionally, the preset volume ranges from 50 μL to 200 μL, the preset temperature ranges from 22° C. to 30° C., and the constant parameter ranges from 95 to 120.

[0036] Optionally, the device is further used to control the absorber to absorb the lower air column.

[0037] Optionally, the medium to be laid includes agar.

[0038] Optionally, the speed at which the absorber absorbs the upper air column, the medium to be laid and the lower air column is the same preset speed.

[0039] Optionally, the preset speed ranges from 70 μL / s to 100 μL / s.

[0040] Optionally, the volume of the lower air column ranges from 2 μL to 4 μL.

[0041] Based on the above-mentioned control method for laying a medium, the present application also discloses a storage medium on which a computer program is stored, characterized in that when the program is executed by a processor, one or more steps of the above-mentioned control method for laying a medium are implemented.

[0042] The present application discloses a control method, device and storage medium for laying a medium. A suction control signal is received, and the sucker is controlled to suck the upper air column and the medium to be laid in sequence according to the suction control signal, wherein the volume of the upper air column is positively correlated with the viscosity of the medium to be laid and negatively correlated with the volume of the medium to be laid. A release control signal is then received, and the sucker is controlled to release the medium to be laid according to the release control signal. The method described in the present application sucks an air column into the sucker in advance, and when released, a certain pressure is provided by the air column to push out the medium to be laid, thereby controlling the generation of bubbles in the medium to be laid, so that the cell reproduction in the culture medium can be clearly observed when no bubbles are generated. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0044] Figure 1 A schematic flow chart of a method for controlling laying media disclosed in an embodiment of the present application;

[0045] Figure 2 A schematic diagram of the structure of the extractor disclosed in an embodiment of the present application;

[0046] Figure 3 This is a flow chart of another method for controlling laying media disclosed in an embodiment of the present application;

[0047] Figure 4a This is a graph showing the experimental results when the ambient temperature is 27°C as disclosed in the examples of this application;

[0048] Figure 4b This is a graph showing the experimental results when the ambient temperature is 23°C as disclosed in the examples of this application;

[0049] Figure 5 This is a schematic structural diagram of a control device for laying media disclosed in an embodiment of the present application. DETAILED DESCRIPTION

[0050] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0051] Example 1: This application discloses a control method for laying a medium.

[0052] For details, please refer to Figure 1 A method for controlling the laying of a medium disclosed in this embodiment includes the following steps:

[0053] Step 101: Receive a suction control signal.

[0054] In the method described in this embodiment, a set ambient temperature and a preset volume of the medium to be laid are first received. Based on the ambient temperature and the preset volume of the medium to be laid, the volume of the upper air column is determined as a first volume. Alternatively, the suction control signal may include the volume of the upper air column to be sucked, the volume of the medium to be laid, and the suction speed.

[0055] In the method described in this embodiment, the medium to be laid can be agar. The volume of the upper air column is positively correlated with the viscosity of the medium to be laid and negatively correlated with the volume of the medium to be laid. The viscosity of the medium to be laid is also related to the ambient temperature, that is, the volume of the upper air column decreases as the ambient temperature rises and decreases as the volume of the medium to be laid increases. Therefore, when the ambient temperature is within a preset temperature range, the relationship between the first volume, the preset volume, and the ambient temperature is:

[0056] V1=-1 / 4*V-2*T+c;

[0057] Wherein, V1 represents the first volume, V represents the preset volume, both in μL. T represents the ambient temperature in °C. c is a constant parameter. The preset temperature range may be 22°C to 30°C, the preset volume range may be 50 μL to 200 μL, and the constant parameter range may be between 95 and 120.

[0058] For example, as an optional method, if the ambient temperature is set to 27°C and the medium to be laid is 100 μL of agar, the volume of the upper air column (the first volume) is 16 μL to 41 μL. The formula described in this embodiment can calculate the volume of the upper air column when the ambient temperature and the preset volume of the medium to be laid are known, making it easier to adjust the volume of the upper air column accordingly under different circumstances, accurately laying the medium, and ensuring the accuracy of subsequent experiments.

[0059] Step 102: Control the sucker to suck the upper air column and the medium to be laid in sequence according to the sucking control signal.

[0060] In the method described in this embodiment, the aspirator may include an automated suction head. The aspiration speed of the aspirator may be maintained at a constant preset speed, that is, the aspiration speed when aspirating the air column or the medium to be laid is the same, and its value range may be 70 μL / s to 100 μL / s. After controlling the aspirator to sequentially aspirate the upper air column and the medium to be laid according to the aspiration control signal, the aspirator may also be controlled to aspirate the lower air column to prevent leakage of the medium to be laid. As an optional method, its volume value range may be 2 μL to 4 μL.

[0061] Step 103: Receive a release control signal.

[0062] In the method described in this embodiment, the release control signal may include a release volume and a release speed. As an optional method, the release volume may be equal to the sum of the volume of the upper air column, the volume of the medium to be laid, and the volume of the lower air column, that is, the medium to be laid is completely released. Correspondingly, it may also be greater than the volume of the lower air column and less than the sum of the volume of the upper air column and the volume of the medium to be laid, that is, the medium to be laid is not completely released. The release speed may be equal to the suction speed or may not be equal to the suction speed, as long as the medium to be laid is released without affecting the experimental results. No specific limitation is made to the release speed here.

[0063] Step 104: Control the sucker to release the medium to be laid according to the release control signal.

[0064] In the method described in this embodiment, during the release process, as an optional method, the medium to be laid can be completely released. Alternatively, the medium to be laid can be partially released. In this case, there is residual medium to be laid in the aspirator, and the lower air column needs to be re-absorbed after release to prevent contamination of the medium to be laid during reabsorption. When the medium to be laid is completely released, the upper air column is used to provide a certain pressure to impact the medium to be laid, thereby controlling the generation of bubbles in the medium to be laid, thereby reducing bubbles in the culture medium.

[0065] The method described in this embodiment can calculate the volume of the upper air column according to a formula when the ambient temperature and the volume of the medium to be laid are known. The method controls the extractor to sequentially draw in the upper air column and the medium to be laid according to a received suction control signal, and then controls the extractor to release the medium to be laid according to a release control signal, completing the medium laying process. The method employs a method in which the air column is drawn into the extractor, and upon release, the air column strikes the medium to be laid, controlling the bubbles generated by the medium to be laid, thereby enabling clear observation of cell proliferation in the culture medium. Furthermore, the volume of the upper air column can be accurately calculated and adjusted based on changes in parameters such as ambient temperature, facilitating experiments in a variety of environments.

[0066] This application discloses a schematic diagram of the structure of the extractor during the extraction process. Figure 2 This is a schematic diagram of the structure of the extractor disclosed in the embodiment of this application, as shown in FIG. Figure 2 As shown:

[0067] 201 is a pipette, which may include an automated pipette head.

[0068] 202 is the upper air column sucked by the sucker, which is used to knock out the medium to be laid.

[0069] 203 is the medium to be laid sucked by the sucker.

[0070] 204 is the lower air column sucked by the sucker.

[0071] Whether the extractor described in this application is upright or inverted, the air column farther from the extractor outlet (tip) is the upper air column, and the air column closer to the extractor outlet is the lower air column. During extraction, the upper air column, the medium to be laid, and the lower air column are drawn in from the extractor outlet in sequence. During release, the lower air column, the medium to be laid, and the upper air column are discharged from the extractor outlet in sequence.

[0072] Example 2: This application discloses another method for controlling the laying of media. Figure 3 , the method described in this embodiment is introduced with respect to the specific process of laying the medium.

[0073] Step 301: Receive an aspiration control signal and set the aspiration speed to 70 μL / s.

[0074] In the method described in this embodiment, an ambient temperature of 27°C and a 100 μL volume of agar are first assumed for the medium to be laid. The volume of the upper air column is calculated to be between 16 μL and 41 μL according to the formula. Alternatively, the volume of the upper air column can be set to 30 μL. Therefore, the aspiration control signal includes aspirating 30 μL of the upper air column and 100 μL of agar.

[0075] Step 302: Control the aspirator to sequentially aspirate the upper air column and agar according to the aspiration control signal.

[0076] In the method described in this embodiment, the aspirator is controlled to sequentially aspirate the upper air column with a volume of 30 μL and the agar with a volume of 100 μL.

[0077] Step 303: Control the aspirator to absorb the lower air column with a volume of 3 μL.

[0078] Step 304: Receive a release control signal and set the release rate to 80 μL / s.

[0079] In the method described in this embodiment, the release control signal includes completely releasing the lower air column, the upper air column and the agar with a total volume of 133 μL.

[0080] Step 305: Control the aspirator to completely release the agar according to the release control signal.

[0081] The method described in this embodiment uses a known ambient temperature and the volume of the medium to be laid. The volume of the upper air column is calculated using a formula. The method then controls the extractor to sequentially draw in the upper air column and the medium to be laid in response to a received suction control signal. The extractor then releases the medium to be laid in response to a release control signal, completing the laying process. The volume of the upper air column can be accurately calculated and adjusted based on changes in parameters such as ambient temperature, resulting in more accurate results.

[0082] This application discloses the experimental result table and experimental result graph of the plaque subagar verification experiment. Among them, Table 1 is the experimental result table when the ambient temperature is 27°C, and the experimental results are shown in the following table:

[0083] Table 1 Media laying conditions at 27℃

[0084]

[0085]

[0086] Figure 4a The experimental results at 27℃ are shown in the figure. Figure 4a As shown in the figure, when the ambient temperature is 27°C and the volume of the medium to be laid is 100μL, 120μL, 140μL and 160μL respectively, different experimental results can be obtained by changing the volume of the upper air column. Among them, the experimental result with no bubbles and no vacancies is the optimal result, that is, the volume of the upper air column at this time is the optimal volume. The cause of the vacancies may be related to the state of the agar, such as the solidification of the agar, or to the aspirator, such as the failure of the aspirator resulting in the agar in the aspirator not being pumped out. It can be concluded that, under the same ambient temperature, the optimal volume of the upper air column decreases as the volume of the medium to be laid increases.

[0087] Table 2 is the experimental results when the ambient temperature is 23°C. The experimental results are shown in the following table:

[0088] Table 2 Media laying conditions at 23°C

[0089]

[0090]

[0091] Figure 4b The experimental results at 23℃ are shown in the figure. Figure 4b As shown in the figure, when the ambient temperature is 23℃ and the volume of the medium to be laid is 100μL, 120μL, 140μL and 160μL respectively, different experimental results can be obtained by changing the volume of the upper air column. Figure 4a Combined with Figure a4, it can be concluded that under different ambient temperatures, the optimal volume of the upper air column decreases with increasing temperature.

[0092] Based on the control method for laying a medium disclosed in the above embodiment, this embodiment correspondingly discloses a control device for laying a medium, such as Figure 5 As shown, it includes: an absorption signal receiving module 501, an absorption control module 502, a release signal receiving module 503, and a release control module 504;

[0093] The suction signal receiving module 501 is used to receive the suction control signal;

[0094] The suction control module 502 is used to control the sucker to suck the upper air column and the medium to be laid in sequence according to the suction control signal; the volume of the upper air column is positively correlated with the viscosity of the medium to be laid, and negatively correlated with the volume of the medium to be laid;

[0095] The release signal receiving module 503 is used to receive a release control signal;

[0096] The release control module 504 is configured to control the sucker to release the medium to be laid according to the release control signal.

[0097] Optionally, the device further comprises: a volume receiving module and a first volume determining module;

[0098] The volume receiving module is used to receive the ambient temperature and the preset volume of the medium to be laid;

[0099] The first volume determination module is used to determine the volume of the upper air column as a first volume according to the ambient temperature and the preset volume; the viscosity of the medium to be laid is related to the ambient temperature.

[0100] Optionally, the first volume determination module includes:

[0101] The relationship determination submodule is configured to determine that when the ambient temperature is within a preset temperature range, the relationship between the first volume, the preset volume, and the ambient temperature is:

[0102] V1=-1 / 4*V-2*T+c;

[0103] Wherein, V1 represents the first volume, V represents the preset volume, and both are in μL; T represents the ambient temperature, and its unit is °C; and c is a constant parameter.

[0104] Optionally, the preset volume ranges from 50 μL to 200 μL, the preset temperature ranges from 22° C. to 30° C., and the constant parameter ranges from 95 to 120.

[0105] Optionally, the device is further used to control the absorber to absorb the lower air column.

[0106] Optionally, the medium to be laid includes agar.

[0107] Optionally, the speed at which the absorber absorbs the upper air column, the medium to be laid, and the lower air column is the same preset speed.

[0108] Optionally, the preset speed ranges from 70 μL / s to 100 μL / s.

[0109] Optionally, the volume of the lower air column ranges from 2 μL to 4 μL.

[0110] Based on the control method for laying a medium disclosed in the above embodiment, this embodiment correspondingly discloses a storage medium on which a computer program is stored. When the program is executed by a processor, one or more steps of the control method for laying a medium are performed.

[0111] The embodiments in this specification are described in a progressive manner. As for the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the description of the methods.

[0112] It should also be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article, or device comprising the element.

[0113] The steps of the methods or algorithms described in conjunction with the embodiments disclosed herein may be implemented directly using hardware, a software module executed by a processor, or a combination of the two. The software module may be placed in a random access memory (RAM), internal memory, read-only memory (ROM), electrically programmable ROM, electrically erasable programmable ROM, registers, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art.

[0114] The features described in the embodiments of this specification can be replaced with each other or combined to enable professional and technical personnel in this field to implement or use this application.

[0115] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present application. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application is not limited to the embodiments shown herein, but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for controlling laying of a medium, characterized in that: include: receiving an absorption control signal; The suction device is controlled to sequentially suck the upper air column and the medium to be laid according to the suction control signal; the volume of the upper air column is positively correlated with the viscosity of the medium to be laid and negatively correlated with the volume of the medium to be laid; receiving a release control signal; controlling the sucker to release the medium to be laid according to the release control signal; Before receiving the absorption signal, the method further includes: receiving an ambient temperature and a preset volume of the medium to be laid; According to the ambient temperature and the preset volume, the volume of the upper air column is determined to be a first volume; the viscosity of the medium to be laid is related to the ambient temperature; When the ambient temperature is within a preset temperature range, the relationship between the first volume, the preset volume, and the ambient temperature is: V1=-1 / 4*V-2*T+c; Among them, V1 represents the first volume, V represents the preset volume, and the unit is μL; T represents the ambient temperature, and its unit is ℃; c is a constant parameter; the value range of the preset volume is 50μL to 200μL, the preset temperature range is 22℃ to 30℃, and the value range of the constant parameter is 95 to 120.

2. The method according to claim 1, characterized in that After the sucker is controlled to suck the upper air column and the medium to be laid in sequence according to the suction control signal, the method further includes: controlling the sucker to suck the lower air column.

3. The method according to claim 1, characterized in that The medium to be laid includes agar.

4. The method according to claim 2, characterized in that The speed at which the absorber absorbs the upper air column, the medium to be laid and the lower air column is the same preset speed.

5. The method according to claim 4, characterized in that The preset speed ranges from 70 μL / s to 100 μL / s.

6. The method according to claim 2, characterized in that The volume of the lower air column ranges from 2 μL to 4 μL.

7. A control device for laying a medium, characterized in that: The device comprises a controller, wherein the controller is used to execute the control method for laying media according to any one of claims 1 to 6.

8. A storage medium having a computer program stored thereon, characterized in that: When the program is executed by a processor, the steps of the method according to any one of claims 1 to 6 are implemented.

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