A high-quality and stable roasted slice dispersion method and device

Abstract

The present invention discloses a high-quality, stable dispersing method and device for baking wafers. During baking, the indoor temperature is 25-30°C and the indoor humidity is 45-65%. The baking temperature is constant and the baking time is 40-60 seconds. The baking is performed by placing a titration glass slide on a water-absorbing material. The titration glass slide is tilted on the water-absorbing material, i.e., one end of the titration glass slide on the water-absorbing material is higher than the other end. By controlling the indoor temperature and humidity, as well as the baking temperature and duration, the present invention performs open, batch baking. This solves the problems of limited space, high workload, and long baking times caused by having to bake the slides one by one in a small space.

Description

Technical Field

[0001] The invention belongs to the technical field of chromosome roasted slice dispersion, and in particular relates to a high-quality and stable roasted slice dispersion method and device. Background Art

[0002] Chromosome dispersion technology involves chemically treating cells, applying a method to eliminate the cell membrane and intracellular material, leaving only the chromosomes within the nucleus. The chromosomes must be evenly dispersed. Existing chromosome dispersion devices primarily create a constant temperature and humidity environment within the device, allowing the methanol in the chromosome cell suspension dropped onto a glass slide to evaporate, thereby hydrolyzing the cells' proteins, causing the outer layer of the cells to disappear and releasing the chromosomes. Once evenly dispersed, the slide is dried under the influence of temperature, fixing the chromosomes on the slide. However, the methanol volatilization process and the process of cellular protein hydrolysis to release and disperse the chromosomes are relatively slow. Existing technology bakes the slides in about five minutes at most. Patent application number CN201420406046.9 discloses a timed humidification and air-blowing chromosome dispersion device. By providing a timed humidification and air-blowing device that blows humidified air vertically onto the slide at a predetermined time, the chromosome dispersion time is greatly shortened, ensuring both effective chromosome dispersion and significantly increasing work efficiency. The baking time is within 30 seconds. However, when processing glass slides, the device has complicated operations of loading and unloading slides, and can only process one slide at a time, and the number of slides processed is very limited. In addition, the temperature and humidity of the device are difficult to control as a whole, and the quality of chromosome dispersion has extremely high requirements for temperature and humidity. Therefore, the quality of the baked slides and the overall efficiency cannot be guaranteed. Summary of the Invention

[0003] In view of the shortcomings of the existing technology, the first purpose of the present invention is to provide a high-quality and stable roasted slice dispersion method, and the second purpose is to provide a roasted slice device using the above-mentioned roasted slice dispersion method to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above first purpose, the present invention provides the following technical solutions:

[0005] The invention discloses a high-quality and stable baking sheet dispersion method. The indoor temperature is 25-30 DEG C and the indoor humidity is 45-65%. The baking temperature is constant and the baking time is 40-60 seconds. The baking is carried out by placing a titration glass slide on a water-absorbing material.

[0006] Furthermore, the baking temperature is 36-38°C.

[0007] Furthermore, the temperature of the baking sheet is 37°C.

[0008] Furthermore, the titration glass slide is in an inclined shape on the water-absorbing material, that is, one end of the titration glass slide on the water-absorbing material is higher and the other end is lower.

[0009] Furthermore, the water-absorbing material is filled with water, and the specific water content is determined by the water content not directly overflowing when the water-absorbing material is left standing.

[0010] Furthermore, the water content of the water absorbing material is 40%-80%.

[0011] Furthermore, the water absorbing material is preheated to 36-38°C.

[0012] Furthermore, the preheating time is 30-90s.

[0013] Furthermore, the titration slide is an overnight drop slide, and the preparation of the overnight drop slide is as follows: the harvested specimen is fixed with a fixative for a period of time, and then centrifuged to obtain a certain amount of cell sediment, the cell sediment is mixed with the fixative to prepare a certain amount of cell suspension, and the cell suspension is titrated onto a water-washed slide to obtain an overnight drop slide.

[0014] Furthermore, the time is generally one night, specifically 5-12 hours.

[0015] Furthermore, the prepared volume of the cell suspension is 2-8 ml.

[0016] Furthermore, the titration amount of the cell suspension is 0.4-0.6 ml.

[0017] Furthermore, the water-washed glass slide is a water-washed glass slide that has been pre-cooled to 4°C.

[0018] Furthermore, after the cell suspension is dripped onto the water-washed glass slide, the water on the glass slide is wiped dry.

[0019] By adopting the above technical solution to bake the slides, the open and batch baking can be carried out by controlling the temperature, humidity, temperature and duration of the baking in the room, thus solving the problem of limited space, large workload and long baking time caused by placing the slides in a small space and baking them one by one. After the temperature, humidity and temperature of the baking slides are set in the room, the titration slides can be placed in batches at an angle on the water-absorbing material at 37°C. After the slides are baked for tens of seconds, the baked slides can be collected one by one. The use of the water-absorbing material allows the slides to contact the water-absorbing material. The water-absorbing material can make the temperature inside and the temperature and humidity around it constant and lasting through the moisture contained in it, which facilitates further control of the actual baking temperature and humidity around the slides when baking. Since the baking time of each slide is tens of seconds and they are placed and collected in sequence, the baking time of each slide mostly overlaps. Therefore, according to the number of baked slides, the time to complete the batch baking is only a few minutes, which greatly improves the number and time of baking slides. Furthermore, in the present method, the harvested specimen is fixed overnight with a fixative and then centrifuged the next day to obtain overnight slides. The overnight slides serve three purposes: first, to stabilize the morphological structure of the chromosomes; second, to remove the effects of the extrachromosomal material on chromosome unfolding on the slide; and third, to remove red blood cell debris, resulting in a clearer background on the specimen slide. Using pre-cooled, 4°C washed slides for slide placement facilitates even dispersion of cells. After slide placement, the slides are promptly wiped dry to prevent the slightest movement of the slides from washing away the chromosomes. During drying, the slides are tilted on a water-absorbing material. This allows for the evaporation of water vapor from the absorbent material to control the humidity and temperature around the slides, facilitating chromosome dispersion. The tilted placement facilitates the diffusion of humidity from the slides, increasing the degree of chromosome dispersion. The presence and contact of the absorbent material prevents excessive drying of the slides and absorbs moisture from the surrounding area. The drying time is controlled to approximately 50 seconds. Once the slides are dry, the backs of the slides are wiped dry, ensuring a clear background, even cell distribution, and good dispersion. The chromosome unfolding process during baking is as follows: 0-10 seconds, the suspension is placed on the slide, the cells drift, and then become fixed to the slide; 10-25 seconds, no visible chromosome unfolding; 25-50 seconds, chromosome unfolding, and the last 5 seconds, the fastest unfolding. After baking is complete, chromosomes become visible 50-90 seconds later.

[0020] The second object of the present invention is to provide a slice baking device using the above-mentioned slice baking dispersion method, and the specific technical solution is as follows:

[0021] A slice baking device comprises a heating platform and a water absorbing material placed on the heating platform.

[0022] Furthermore, the water-absorbing material is L-shaped or concave-shaped.

[0023] Furthermore, the water-absorbing material is concave, and a partition plate is provided in the concave groove.

[0024] Furthermore, the water-absorbing material is a flexible water-absorbing material.

[0025] Furthermore, the heating platform is heated by water bath or electricity, and can be a sheet baking device or a water bath.

[0026] Furthermore, it also includes a blowing device. The blowing device can be a fan. There can be multiple blowing devices, evenly arranged on one side of the heating platform, so that it can blow air to the other side of the platform.

[0027] Furthermore, a timer is also included. The timer can be a stopwatch.

[0028] Furthermore, a thermometer is included. The thermometer can be a constant temperature thermometer. There can be multiple constant temperature thermometers, evenly distributed on the heating platform to monitor the temperature of the platform and the surrounding area.

[0029] Furthermore, the device further comprises a hygrometer. There can be multiple hygrometers evenly distributed on the heating platform to monitor the humidity of the platform and its surroundings.

[0030] Furthermore, the system further comprises an exhaust device. The exhaust device may be a fume hood. The exhaust port of the exhaust device is located on one side of the heating platform, allowing it to draw air away from the other side of the platform. Preferably, the blowing device and the exhaust port of the exhaust device are symmetrically arranged, that is, the blowing device is located on one side of the heating platform, and the exhaust port of the exhaust device is located on the opposite side of the heating platform from where the blowing device is located.

[0031] By adopting the above technical solution, the glass slide is placed on the water-absorbing material instead of being placed directly on the heating platform for baking, so that the temperature, humidity and time of baking the slide are easier to control. The water-absorbing material is set to have a protrusion, that is, a high platform, so that when baking the slide, there is a gap between the glass slide and the water-absorbing material, which can prevent the glass slide from being over-dried and absorb moisture around the glass slide. The heating platform, the blowing device and the timer are combined, firstly, to keep the surrounding environment at the required temperature and humidity, secondly, to recycle the surrounding water vapor, and thirdly, to control the time the glass slide is placed for baking. Preferably, a display screen can be set to display the data of various thermometers, hygrometers, timers, etc., to monitor the temperature, humidity and time in real time, to prevent problems such as uneven heating or humidity or time on the surface of the heating platform, which will lead to uneven distribution of cells on the glass slide, affect the dispersion of cells on the glass slide, and over-baking.

[0032] The present invention has the following beneficial effects:

[0033] 1. The baking and dispersing method of the present invention has easy-to-control environmental conditions, simple operation, high efficiency, greatly saves time, speeds up work efficiency by more than 30%, improves the quality of slice preparation, reduces the specimen return rate, and lays the foundation for providing higher quality test reports for clinical practice.

[0034] 2. The slice baking device of the present invention occupies a small area, saves space, and is easy to control the local environment. It is cheaper than existing similar equipment (about 1 million of the same type). BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of a baking sheet according to Example 1 of the present invention;

[0036] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0037] Figure 3 This is a schematic diagram of the placement of the baking sheet in Example 2 of the present invention;

[0038] Figure 4 This is a schematic diagram of the placement of the baking sheet in Example 3 of the present invention;

[0039] In the figure: 1. Heating platform; 2. Water-absorbing material; 3. Partition plate; 4. Blowing device; 5. Timer; 6. Thermometer; 7. Hygrometer; 8. Exhaust device; 801. Exhaust vent. DETAILED DESCRIPTION

[0040] The present invention will be described in detail below with reference to the accompanying drawings and embodiments.

[0041] A high-quality and stable baking slide dispersion method is provided. The indoor temperature is 25-30°C and the indoor humidity is 45-65%. The baking temperature is constant and the baking time is 40-60s. The baking is performed by placing a titration glass slide on a water-absorbing material 2.

[0042] In some embodiments, the baking temperature is 36-38°C, preferably 37°C.

[0043] In some embodiments, the titration glass slide is placed on the water-absorbing material 2 in an inclined shape, that is, one end of the titration glass slide on the water-absorbing material 2 is higher and the other end is lower.

[0044] In some embodiments, the water-absorbing material 2 is filled with water, and the specific water content is determined by whether the water contained in the water-absorbing material 2 does not directly overflow when the water-absorbing material 2 is left stationary.

[0045] In some embodiments, the moisture content of the water-absorbing material 2 is 40%-80%.

[0046] In some embodiments, the water absorbing material 2 is preheated to 36-38°C, preferably 37°C.

[0047] In some embodiments, the preheating time is 30-90 seconds.

[0048] In some embodiments, the titration slide is an overnight drop slide. The preparation of the overnight drop slide is as follows: the harvested specimen is fixed with a fixative for a period of time, and then centrifuged to obtain a certain amount of cell sediment, the cell sediment is mixed with the fixative to prepare a certain amount of cell suspension, and the cell suspension is titrated onto a water-washed slide to obtain an overnight drop slide.

[0049] In some embodiments, the time period is generally one night, specifically 5-12 hours.

[0050] In some embodiments, the volume of the cell suspension is 2-8 ml.

[0051] In some embodiments, the titer of the cell suspension is 0.4-0.6 ml.

[0052] In some embodiments, the water-washed glass slides are water-washed glass slides that have been pre-cooled at 4°C.

[0053] In some embodiments, after the cell suspension is dripped onto the water-washed glass slide, the water on the glass slide is wiped dry.

[0054] The present invention also provides a slice baking device using the above-mentioned slice baking dispersion method, comprising a heating platform 1 and a water-absorbing material 2 placed on the heating platform 1.

[0055] In some embodiments, the water-absorbing material 2 is L-shaped.

[0056] In some embodiments, the water-absorbing material 2 is concave.

[0057] In some embodiments, a partition plate 3 is provided in the concave groove of the water-absorbing material 2 .

[0058] In some embodiments, the water-absorbing material 2 is a flexible water-absorbing material.

[0059] In some embodiments, the heating platform 1 is heated by water bath or electricity, and can be a sheet baking machine or a water bath.

[0060] In some embodiments, a blowing device 4 is further included. The blowing device 4 can be a fan.

[0061] In some embodiments, there may be multiple blowing devices 4, which are evenly arranged on one side of the heating platform 1 so as to be able to blow air toward the other side of the platform.

[0062] In some embodiments, a timer 5 is further included. The timer 5 may be a stopwatch.

[0063] In some embodiments, a thermometer 6 is further included.

[0064] In some embodiments, the thermometer 6 may be a constant temperature thermometer.

[0065] In some embodiments, there may be multiple constant temperature thermometers evenly distributed on the heating platform 1 to monitor the temperature of the platform and the surrounding area.

[0066] In some embodiments, a hygrometer 7 is further included.

[0067] In some embodiments, there may be multiple hygrometers 7 evenly distributed on the heating platform 1 to monitor the humidity of the platform and its surroundings.

[0068] In some embodiments, an exhaust device 8 is further included. The exhaust device 8 can be a fume hood.

[0069] In some embodiments, the exhaust port 801 of the exhaust device 8 is located on one side of the heating platform 1 so that it can draw air from the other side of the platform. Preferably, the blowing device 4 and the exhaust port of the exhaust device 8 are symmetrically arranged, that is, the blowing device 4 is located on one side of the heating platform 1, and the exhaust port 801 of the exhaust device 8 is located on the opposite side of the heating platform 1 from where the blowing device 4 is located.

[0070] Example 1

[0071] A high quality and stable baking device, such as Figure 1 、 2 As shown, the apparatus comprises a heating platform 1 and a water-absorbing material 2 placed on the heating platform 1. The water-absorbing material 2 is concave, with a partition 3 positioned within the concave groove. The water-absorbing material is flexible. The heating platform 2 is heated in a water bath, specifically a water bath. The water-absorbing material 2 is placed on the top plate of the water bath, and an opening is provided on the top plate to facilitate the escape of water vapor.

[0072] Specifically, Figure 1 As shown, the heating platform 1 further comprises a blowing device 4. The blowing device 4 can be a fan. There can be multiple blowing devices 4, which are evenly arranged on one side of the heating platform 1 so that they can blow air to the other side of the platform.

[0073] Specifically, Figure 1 As shown, a timer 5 is also included. The timer 5 can be a stopwatch.

[0074] Specifically, Figure 1 As shown, the heating platform 1 further comprises a thermometer 6. The thermometer 6 can be a constant temperature thermometer. There can be multiple constant temperature thermometers, evenly distributed on the heating platform 1, to monitor the platform and the surrounding temperature.

[0075] Specifically, Figure 1 As shown, it also includes a hygrometer 7. There can be multiple hygrometers 7, which are evenly distributed on the heating platform 1 to monitor the humidity of the platform and the surrounding area.

[0076] Specifically, Figure 1As shown, the heating platform further includes an exhaust device 8. The exhaust device 8 can be a fume hood. The exhaust port 801 of the exhaust device is arranged on one side of the heating platform so that it can extract the wind on the other side of the platform. Preferably, the blowing device 4 and the exhaust port 801 of the exhaust device 8 are arranged symmetrically.

[0077] Example 2

[0078] A high-quality and stable baking device, which is different from embodiment 1 in that Figure 3 As shown, the water-absorbing material 2 is concave, and no partition plate 3 is provided in the concave groove. The heating platform 2 adopts electric heating, and can be a baking device. Its configuration is the same as that of embodiment 1.

[0079] Example 3

[0080] A high-quality and stable baking device, which is different from embodiment 1 in that Figure 4 As shown, the water-absorbing material 2 is L-shaped. Its configuration is the same as that of embodiment 1.

[0081] Example 4

[0082] The invention discloses a high-quality and stable baking slice dispersion method. When baking the slices, the indoor temperature is 27° C. and the indoor humidity is 55%. The baking slice temperature is kept constant at 37° C. and the baking time is 50 seconds.

[0083] Specifically, the baking step involves placing a titration glass slide on the water-absorbing material 2 for baking.

[0084] Specifically, the titration glass slide is placed on the water-absorbing material 2 in an inclined shape, that is, one end of the titration glass slide on the water-absorbing material 2 is higher and the other end is lower.

[0085] Specifically, the water absorbing material 2 is filled with water, and the specific water content is determined by the water absorbing material 2 not overflowing directly when the water absorbing material 2 is left standing.

[0086] Specifically, the moisture content in the water-absorbing material 2 is 60%.

[0087] Specifically, the water absorbing material 2 is preheated to 37°C.

[0088] Specifically, the preheating time is 60s.

[0089] Specifically, the titration slide is an overnight drop slide. The preparation of the overnight drop slide is as follows: the harvested specimen is fixed with a fixative for a period of time, and then centrifuged to obtain a certain amount of cell sediment, the cell sediment is mixed with the fixative to form a certain amount of cell suspension, and the cell suspension is titrated onto a water-washed slide to obtain an overnight drop slide.

[0090] Specifically, the time is generally one night, specifically 8 hours.

[0091] Specifically, the prepared volume of the cell suspension is 6 ml.

[0092] Specifically, the titration amount of the cell suspension was 0.5 ml.

[0093] Specifically, the water-washed slides are water-washed slides that have been pre-cooled to 4°C.

[0094] Specifically, after the cell suspension is dripped onto the water-washed glass slide, the water on the glass slide is wiped dry.

[0095] The method of this embodiment can be used to perform the slice baking operation using any of the devices in Examples 1-3.

[0096] Example 5

[0097] The invention discloses a high-quality and stable baking slice dispersion method. When baking the slices, the indoor temperature is 25°C and the indoor humidity is 45%. The baking slice temperature is kept constant at 36°C and the baking time is 60s.

[0098] Specifically, the baking step involves placing a titration glass slide on the water-absorbing material 2 for baking.

[0099] Specifically, the titration glass slide is placed on the water-absorbing material 2 in an inclined shape, that is, one end of the titration glass slide on the water-absorbing material 2 is higher and the other end is lower.

[0100] Specifically, the water absorbing material 2 is filled with water, and the specific water content is determined by the water absorbing material 2 not overflowing directly when the water absorbing material 2 is left standing.

[0101] Specifically, the moisture content in the water-absorbing material 2 is 40%.

[0102] Specifically, the water absorbing material 2 is preheated to 36°C.

[0103] Specifically, the preheating time is 90s.

[0104] Specifically, the titration slide is an overnight drop slide. The preparation of the overnight drop slide is as follows: the harvested specimen is fixed with a fixative for a period of time, and then centrifuged to obtain a certain amount of cell sediment, the cell sediment is mixed with the fixative to form a certain amount of cell suspension, and the cell suspension is titrated onto a water-washed slide to obtain an overnight drop slide.

[0105] Specifically, the time is generally one night, specifically 5 hours.

[0106] Specifically, the prepared volume of the cell suspension is 2 ml.

[0107] Specifically, the titration amount of the cell suspension was 0.4 ml.

[0108] Specifically, the water-washed slides are water-washed slides that have been pre-cooled to 4°C.

[0109] Specifically, after the cell suspension is dripped onto the water-washed glass slide, the water on the glass slide is wiped dry.

[0110] The method of this embodiment can be used to perform the slice baking operation using any of the devices in Examples 1-3.

[0111] Example 6

[0112] The invention discloses a high-quality and stable baking slice dispersion method. When baking the slices, the indoor temperature is 30°C and the indoor humidity is 65%. The baking slice temperature is kept constant at 38°C and the baking time is 40 seconds.

[0113] Specifically, the baking step involves placing a titration glass slide on the water-absorbing material 2 for baking.

[0114] Specifically, the titration glass slide is placed on the water-absorbing material 2 in an inclined shape, that is, one end of the titration glass slide on the water-absorbing material 2 is higher and the other end is lower.

[0115] Specifically, the water absorbing material 2 is filled with water, and the specific water content is determined by the water absorbing material 2 not overflowing directly when the water absorbing material 2 is left standing.

[0116] Specifically, the water content in the water-absorbing material 2 is 80%.

[0117] Specifically, the water absorbing material 2 is preheated to 38°C.

[0118] Specifically, the preheating time is 30 seconds.

[0119] Specifically, the titration slide is an overnight drop slide. The preparation of the overnight drop slide is as follows: the harvested specimen is fixed with a fixative for a period of time, and then centrifuged to obtain a certain amount of cell sediment, the cell sediment is mixed with the fixative to form a certain amount of cell suspension, and the cell suspension is titrated onto a water-washed slide to obtain an overnight drop slide.

[0120] Specifically, the time is generally one night, specifically 12 hours.

[0121] Specifically, the prepared volume of the cell suspension is 8 ml.

[0122] Specifically, the titration amount of the cell suspension was 0.6 ml.

[0123] Specifically, the water-washed slides are water-washed slides that have been pre-cooled to 4°C.

[0124] Specifically, after the cell suspension is dripped onto the water-washed glass slide, the water on the glass slide is wiped dry.

[0125] The method of this embodiment can be used to perform the slice baking operation using any of the devices in Examples 1-3.

[0126] In summary, the above preferred embodiments or other unlisted embodiments of the device or method of the present invention are used to bake the slides, specifically by controlling the temperature, humidity, and baking temperature and time in the room to perform open and batch baking, which solves the problem of limited space, large workload, and long baking time caused by the need to place the slides in a small space for baking one by one. After the method of the present invention sets the temperature, humidity, and baking temperature of the room, the titration slides can be placed in batches at an angle on a water-absorbing material at a constant temperature, preferably 37°C, and the baked slides can be collected one by one after baking for tens of seconds. Since the baking time for each slide is tens of seconds, and they are placed and collected in sequence, the baking time for each slide mostly overlaps. Therefore, based on the number of baked slides, the time to complete batch baking is only a few minutes, which greatly improves the number and time of baking slides. For example, the DB-B2 slide baker (Guohua (Changzhou) Instrument Manufacturing Co., Ltd.) currently used in laboratories has a baking area of 640×200 cm. After placing the absorbent material, it can bake approximately 25 slides in two to three minutes. Compared to the baking device disclosed in patent CN201420406046.9, which bakes one slide in 30 seconds, this greatly improves work efficiency because it takes 750 seconds to bake 25 slides, or approximately twelve minutes. Furthermore, in the method of the present invention, the harvested specimen is fixed with a fixative overnight and then centrifuged and sliced the next day to obtain overnight slides. The purpose of overnight slides is to stabilize the morphological structure of chromosomes, remove the effects of the extrachromosomal material on chromosome unfolding on the slide, and remove red blood cell debris, making the background of the specimen slide clearer. Using 4°C pre-cooled water to wash the slides facilitates even dispersion of cells. After dripping, wipe the water off the slides promptly to prevent slight flow on the slides that could wash away the chromosomes. During drying, slides are placed at an angle on a moisture-absorbing material. This allows for the evaporation of moisture from the absorbent material to control the humidity and temperature around the slide, promoting chromosome dispersion. The tilted position also promotes the diffusion of moisture from the slide, increasing chromosome dispersion. The presence and contact of the moisture-absorbing material prevents excessive drying of the slide and absorbs moisture from the surrounding area. The drying time is controlled to approximately 50 seconds. After the slide is dry, wipe the back of the slide dry. This ensures a clear background and evenly distributes and disperses cells. The chromosome spreading process during baking is as follows: 0-10 seconds, the suspension is placed on the slide, cells drift, and then become fixed to the slide; 10-25 seconds, no visible chromosome spreading; 25-50 seconds, chromosome spreading, and the final 5 seconds, when spreading is most rapid. Chromosomes become visible after baking is complete, 50-90 seconds.

[0127] In addition, the method or device of the present invention is to place the glass slide on the water-absorbing material 2 instead of directly placing it on the heating platform 1 for baking, so that the temperature, humidity and time of baking the slide are easier to control. The water-absorbing material 2 is set to have a convex shape, that is, a high platform, so that when baking the slide, there is a gap between the glass slide and the water-absorbing material 2, which can prevent the glass slide from being excessively dried and absorb moisture around the glass slide. The heating platform 1, the blowing device 4 and the timer 5 are combined, firstly, to keep the surrounding environment at the required temperature and humidity, secondly, to recycle the surrounding water vapor, and thirdly, to control the time the glass slide is placed for baking. Preferably, a display screen can be set to display the data of each thermometer 6, hygrometer 7, timer 6, etc., to monitor the temperature, humidity and time in real time, to prevent problems such as uneven heating or humidity or time on the surface of the heating platform 1, which will lead to uneven distribution of cells on the glass slide, affect the dispersion of cells on the glass slide, and over-baking the slide.

[0128] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the embodiments of the present invention rather than to limit them. Although the embodiments of the present invention are described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the embodiments of the present invention can still be modified or replaced by equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for dispersing high-quality and stable roasted slices, characterized in that: The indoor temperature is 25-30°C and the indoor humidity is 45-65%. The temperature of the baking slide is constant and the baking time is 40-60 seconds. The baking is performed by placing the titration glass slide on a water-absorbing material. The temperature of the baking sheet is 36-38°C; Place the titration slide tilted on the absorbent material, which has been preheated to 36-38°C for 30-90 seconds. The titration glass slide is tilted on the water-absorbing material, that is, one end of the titration glass slide on the water-absorbing material is higher and the other end is lower; The titration slide is an overnight titration slide.

2. A high-quality and stable roasted slice dispersion method according to claim 1, characterized in that: The water-absorbing material is filled with water, and the specific water content is determined by whether the water contained in the water-absorbing material does not directly overflow when the water-absorbing material is left stationary.

3. A high-quality and stable roasted slice dispersion method according to claim 1, characterized in that: The overnight drop slide is prepared by adding a fixative to the harvested specimen for a period of time, then centrifuging to obtain a certain amount of cell sediment, mixing the cell sediment with the fixative to prepare a certain amount of cell suspension, and then titrating the cell suspension onto a water-washed glass slide to obtain the overnight drop slide.

4. The method for dispersing high-quality and stable roasted slices according to claim 3, wherein: The prepared volume of the cell suspension is 2-8 ml.

5. The method for dispersing high-quality and stable roasted slices according to claim 3, wherein: The titration amount of the cell suspension is 0.4-0.6 ml.

6. The method for dispersing high-quality and stable roasted slices according to claim 3, characterized in that: The washed glass slides are pre-cooled to 4°C.

7. A wafer baking device using the high-quality and stable wafer baking dispersion method according to any one of claims 1 to 6, characterized in that: The invention comprises a heating platform (1) and a water-absorbing material (2) placed on the heating platform (1).

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