A kind of extraction method of blueberry seed oil

Abstract

The invention discloses a method for extracting blueberry seed oil, and belongs to the technical field of material extraction. The extraction method of the invention comprises the following steps: putting blueberry seed powder into a supercritical CO2 extraction kettle, performing supercritical CO2 extraction, and obtaining the blueberry seed oil. The method improves the extraction rate of blueberry seed oil, and the method is safe and non-toxic.

Description

Technical Field

[0001] The invention relates to the technical field of material extraction, and in particular to a method for extracting blueberry seed oil. Background Art

[0002] Blueberry, belonging to the Ericaceae family and the genus Vaccinium, is rich in nutrients and has the functions of preventing brain nerve aging, protecting eyesight, strengthening the heart, fighting cancer, softening blood vessels, and enhancing the body's immunity. Blueberry seeds are a by-product of blueberry processing. Blueberry seeds are rich in oils, such as oleic acid, linoleic acid, linolenic acid, etc. These unsaturated fatty acids have great nutritional value.

[0003] There are currently three methods for extracting blueberry seed oil, namely, petroleum ether immersion ultrasonic extraction, organic reagent Soxhlet extraction, and supercritical CO2 extraction technology; the first two methods use organic reagents such as petroleum ether for extraction, which are very harmful to the human body. The extracted blueberry seed oil can only be used for a small amount of analytical and other experiments, and cannot be used for large-scale production, let alone food; supercritical CO2 extraction technology is to directly put blueberry seeds into the extraction kettle, and in a supercritical state, the supercritical fluid is contacted with the substance to be separated, so that the blueberry seed oil can be selectively extracted. The advantage of this method is that CO2 is an inactive gas, and no chemical reaction occurs during the extraction process. It is tasteless, odorless, non-toxic, and very safe. However, the extraction rates of these three types of blueberry seed oil are very low. Summary of the invention

[0004] The invention provides a method for extracting blueberry seed oil. The method improves the extraction rate of the blueberry seed oil and is safe and non-toxic.

[0005] The present invention first provides a method for extracting blueberry seed oil, comprising the following steps:

[0006] The blueberry seed powder is placed in a supercritical CO2 extraction kettle and subjected to supercritical CO2 extraction to obtain the blueberry seed oil.

[0007] In the above extraction method, the temperature of the supercritical CO2 extraction is 40-45°C; specifically, it can be 40 or 45°C.

[0008] In the above extraction method, the pressure of the supercritical CO2 extraction is 20-35 MPa; specifically, it can be 30-35 MPa, 30 or 35 MPa.

[0009] In the above extraction method, the extraction time of the supercritical CO2 extraction is 1 to 2 hours; specifically, it can be 1.5 to 2 hours, 1.5 or 2 hours.

[0010] In the above extraction method, in the supercritical CO2 extraction, the CO2 flow rate is 10 to 20 L / h; specifically, it can be 20 L / h.

[0011] The blueberry seed powder is powder obtained by sieving blueberry seeds through a 30-40 mesh sieve after the blueberry seeds are crushed.

[0012] The present invention further provides blueberry seed oil extracted by the above extraction method.

[0013] The blueberry seed oil extraction method of the present invention is a safe and non-toxic extraction method, and the extraction rate is higher than that of the previous extraction method; animal experiments show that the blueberry seed oil extracted by the present invention has a blood lipid lowering effect and a good effect on cardiac arteriosclerosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is the serum triglyceride content of mice in each group.

[0015] Figure 2 is the total cholesterol content in serum of mice in each group.

[0016] Figure 3 is the serum low-density lipoprotein content of mice in each group.

[0017] Figure 4 is the serum high-density lipoprotein content of mice in each group.

[0018] Figure 5 These are sections of atherosclerotic plaques in the aorta of mice in each group.

[0019] Figure 6 The figures are quantifications of atherosclerotic plaques in the aorta of each group of mice. DETAILED DESCRIPTION

[0020] The present invention is further described in detail below in conjunction with specific embodiments. The given examples are only for illustrating the present invention, but not for limiting the scope of the present invention.

[0021] The experimental methods in the following examples are all conventional methods unless otherwise specified.

[0022] The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0023] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0024] The blueberry seeds in the following examples were purchased from Zhejiang Lanmei Technology Co., Ltd.; the supercritical CO2 extraction instrument was HA120-50-02; and the CO2 was food grade.

[0025] The blueberry seed powder is obtained by crushing blueberry seeds with a powder grinder and passing through a 40-mesh sieve.

[0026] The normal feed used in the following examples is ordinary feed, name: Ordinary Mouse Complete Granular Feed No. 1, manufacturer: Beijing Keao Xieli Feed Co., Ltd.; Beijing Feed Certificate: (2018) 06073.

[0027] Example 1

[0028] 150g of blueberry seed powder was placed in a supercritical CO2 extraction kettle, and the extraction conditions were set as follows: temperature 45°C, extraction pressure 30MPa, CO2 flow rate 20L / h, and extraction time 1.5h. The amount of blueberry seed oil extracted was 22.9g, and the extraction rate was 15.27%.

[0029] Example 2

[0030] 200g of blueberry seed powder was placed in a supercritical CO2 extraction kettle, and the extraction conditions were set as follows: temperature 40°C, extraction pressure 35MPa, CO2 flow rate 20L / h, and extraction time 2h. The obtained blueberry seed oil extraction amount was 32.1g, and the extraction rate was 16.05%.

[0031] Example 3, Activity of Blueberry Seed Oil

[0032] Instruments and materials: high-fat feed (Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd., XT310), positive drug Xuezhikang (Beijing Peking University Weixin Biotechnology Co., Ltd., Z10950029), triglyceride test kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total cholesterol test kit (Nanjing Jiancheng Bioengineering Institute, A111-1-1), high-density lipoprotein test kit (Nanjing Jiancheng Bioengineering Institute, A112-1-1), low-density lipoprotein test kit (Nanjing Jiancheng Bioengineering Institute, A113-1-1), physiological saline (Shijiazhuang Siyao Co., Ltd., H13023200), tissue fixative (Guangzhou Shuopu Biotechnology Co., Ltd., BL539A), centrifuge (Sigma Sigma3K15, USA), powder grinder (Xiaoxiong Electric Co., Ltd. FSJ-A03D1), microplate reader (BIO-TEKEIX800, Meigu Molecular Instrument (Shanghai) Co., Ltd.).

[0033] The mice were divided into groups: 40 Kunming mice weighing 18-22 g were randomly divided into five groups.

[0034] Blank group: fed with normal diet and gavaged with 0.2 mL of water every day;

[0035] Model group: fed with high-fat diet and gavaged with 0.2 mL of water every day;

[0036] High-dose administration group: fed with high-fat diet, gavaged with 0.2 mL of blueberry seed oil prepared in Example 2 every day;

[0037] Low-dose administration group: fed with high-fat diet, gavaged with 0.1 mL of blueberry seed oil prepared in Example 2 every day;

[0038] Positive group: The mice were fed with high-fat diet and intragastrically administered with 90 mg / kg of positive drug (Xuezhikang) every day.

[0039] Mice feeding conditions: Adaptive feeding was carried out 3 days before the start of the experiment. The feeding amount was 100 g of feed per day, and feed and medication were given at 9 am every day. The water bottle was replaced with clean tap water, and the corn cob bedding was changed every two days; the temperature of the feeding environment was controlled at (23±2℃), and the light-dark cycle was 12h.

[0040] Feed and administer medication as required, and stop medication and feeding after 32 days.

[0041] (1) Effect of blueberry seed oil on blood biochemistry in hyperlipidemic mice

[0042] After administration, the mice were anesthetized and blood was collected from their eyeballs. After centrifugation at 4°C and 3000 rpm for 15 min, the supernatant serum was collected for testing.

[0043] Serum was tested using triglyceride test kit, total cholesterol test kit, low-density lipoprotein test kit and high-density lipoprotein test kit.

[0044] (2) Effect of blueberry seed oil on coronary atherosclerotic plaques in hyperlipidemic mice

[0045] After administration, mice were killed and immediately perfused their hearts with ice saline for about 10 minutes. The hearts were then removed and placed in tissue fixative, sliced ​​and stained with O red to obtain aortic sinus slices. The atherosclerotic plaques in the slices were quantified using imageJ.

[0046] (3) Results and discussion

[0047] The results of triglyceride, total cholesterol, low-density lipoprotein and high-density lipoprotein in each group are shown in Figure 1-Figure 4 .Depend on Figure 1-4 It can be seen that there are significant differences between the blank group and the model group in triglycerides, total cholesterol, low-density lipoprotein and high-density lipoprotein, indicating that the modeling is successful. Triglycerides, total cholesterol and low-density lipoprotein, the drug administration group and the positive group have a decreasing trend compared with the model group, and high-density lipoprotein, the drug administration group and the positive group have an increasing trend compared with the model group; triglycerides, the high-dose drug administration group has a significant difference with the model group; low-density lipoprotein, the high-dose drug administration group and the positive group have a significant difference with the model group; high-density lipoprotein, each drug administration group and the positive group have a significant difference with the model group.

[0048] Effect of blueberry seed oil on coronary atherosclerotic plaques in hyperlipidemic mice; the results are shown in Figure 5 and Figure 6 .Depend on Figure 5 and Figure 6 It can be seen that there are significant differences between the blank group and the model group, and each medication group and the positive group have a decreasing trend compared with the model group. Each medication group and the positive group have significant differences compared with the model group.

Claims

1. A method for extracting blueberry seed oil, comprising the following steps: Putting blueberry seed powder into a supercritical CO2 extraction kettle and performing supercritical CO2 extraction to obtain the blueberry seed oil; The conditions of the supercritical CO2 extraction are any of the following: 1) Temperature is 45°C; The pressure is 30 MPa; CO2 flow rate is 20 L / h; Extraction time 1.5 h; 2) Temperature is 40°C; The pressure is 35 MPa; CO2 flow rate is 20 L / h; Extraction time: 2 h.

2. The extraction method according to claim 1, characterized in that: The blueberry seed powder is powder obtained by crushing blueberry seeds and passing through a 30-40 mesh sieve.

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