Fractional release liquid detection device

Abstract

The application provides a graded release liquid detection device, and relates to the technical field of detection equipment. The graded release liquid detection device comprises a detection plate and a power source; the detection plate is provided with a dispersion flow channel, a primary chamber, a pressure stabilizing flow channel and a secondary chamber; the power source is connected with one end of the dispersion flow channel; the other end of the dispersion flow channel is a sample inlet; the dispersion flow channel is connected with a plurality of primary chambers; the plurality of primary chambers are connected with the secondary chamber through communication flow channels; the communication flow channels are provided with three-way chambers; the primary chambers are connected with the three-way chambers through the pressure stabilizing flow channels. The detection equipment comprises the graded release liquid detection device. The technical effect that the valve does not affect the detection result is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection equipment, in particular to a hierarchical release liquid detection device. BACKGROUND

[0002] The upgrading of detection and analysis requirements promotes the updating of existing medical diagnosis and treatment, biological detection, food and drug testing and analysis, etc. Higher requirements are put forward for detection and analysis methods and instruments and equipment, such as rapidness, refinement, miniaturization, portability and integration.

[0003] In the field of sample detection, large-scale automated analysis equipment and medium-sized semi-automatic equipment occupy the market. However, due to their large size and high price, they need to be operated by professional personnel after professional training, and they are usually installed in large hospitals and operated by professional personnel. In addition, they need to be regularly maintained and maintained, which is difficult to meet the needs of on-site detection, rapid detection, home self-detection and other scenarios.

[0004] POCT products gradually entered the public eye because they can perform bedside detection, shorten the sample transmission process and reduce the reporting time. POCT products gradually entered drugstores, homes, hospitals, communities, research institutions and other scenarios, and gradually cultivated people's habit of consuming POCT self-detection equipment. POCT equipment also faces innovation needs such as integration, refinement and portability.

[0005] POCT equipment has different detection items and principles, and its related products and equipment also have different forms. Microfluidic POCT products have become a research hotspot due to their ability to meet rapid, refined and integrated characteristics. In microfluidic products, the sample detection reaction system is not only composed of a single component, but often composed of multiple reaction components. At this time, some special structures or valves need to be set to realize hierarchical release of liquid. Common valves such as wax valves, hydrophobic valves and diaphragm valves. The wax valve needs to be pre-packaged with wax on the flow channel, and in addition, infrared heating or local heating technology is needed to melt the wax valve without affecting other structures, while the wax also has problems such as interfering with the system reaction and detection. The diaphragm valve is often used in three-dimensional structures, and the liquid can continue to move along the flow channel by physically or chemically destroying the membrane, but the membrane destruction is not complete, the membrane composition interferes with the system, and the membrane packaging cost is also high, which limits the popularization and application of the diaphragm valve.

[0006] Therefore, it is an important technical problem for those skilled in the art to provide a hierarchical release liquid detection device that does not affect the detection result. SUMMARY

[0007] The purpose of the present application is to provide a hierarchical release liquid detection device to alleviate the technical problem that the valve affects the detection result in the prior art.

[0008] In a first aspect, an embodiment of the present application provides a hierarchical release liquid detection device, comprising a detection plate and a power source;

[0009] The detection plate is provided with a dispersion flow channel, a primary chamber, a pressure stabilizing flow channel and a secondary chamber, one end of the dispersion flow channel is connected with the power source, and the other end of the dispersion flow channel is provided with a sample inlet;

[0010] The dispersion flow channel is connected with a plurality of primary chambers, the plurality of primary chambers are connected with the secondary chamber through a communication flow channel, and the communication flow channel is provided with a three-way chamber, and the primary chamber is connected with the three-way chamber through the pressure stabilizing flow channel.

[0011] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the dispersion flow channel comprises a shunt flow channel and an inlet flow channel;

[0012] One end of the shunt flow channel is connected with the power source, and the other end of the shunt flow channel is provided with the sample inlet;

[0013] The plurality of primary chambers are connected with the shunt flow channel through the inlet flow channel.

[0014] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the dispersion flow channel further comprises a semicircular chamber, the semicircular chamber is arranged on the shunt flow channel, and the inlet flow channel is connected with the shunt flow channel through the semicircular chamber.

[0015] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the primary chamber comprises a first primary chamber, a second primary chamber and a third primary chamber;

[0016] The first primary chamber, the second primary chamber and the third primary chamber are connected with the shunt flow channel through respective inlet flow channels.

[0017] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the first primary chamber, the second primary chamber and the third primary chamber are respectively connected with the pressure stabilizing flow channel.

[0018] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the secondary chamber is provided with an exhaust passage.

[0019] In combination with the first aspect, an embodiment of the present application provides a possible implementation manner of the first aspect, wherein the hierarchical release liquid detection device further comprises an optical detection module and a light emitting member;

[0020] The light emitting member is located above the secondary chamber, and the optical detection module is located below the secondary chamber.

[0021] With reference to the first aspect, in a possible implementation of the first aspect, the light emitting member includes a fluorescent module, a natural light module, and / or a glow module.

[0022] With reference to the first aspect, in a possible implementation of the first aspect, the power source is a gas pump.

[0023] In a second aspect, an embodiment of the present application provides a detection device including the hierarchical release liquid detection apparatus.

[0024] Advantages:

[0025] The present application provides a hierarchical release liquid detection apparatus, which includes a detection plate and a power source; the detection plate is provided with a dispersion flow channel, a primary chamber, a pressure stabilizing flow channel, and a secondary chamber; the power source is connected to one end of the dispersion flow channel, and the other end of the dispersion flow channel is an injection port; the dispersion flow channel is connected to a plurality of primary chambers, and the plurality of primary chambers are connected to the secondary chamber through a communication flow channel; the communication flow channel is provided with a three-way chamber; and the primary chamber is connected to the three-way chamber through the pressure stabilizing flow channel.

[0026] Specifically, during the detection work, the primer probe lyophilized powder, the PH buffer, and the enzyme mixture are mixed in the primary chamber and the secondary chamber, and a sealing film is covered on the detection plate, so that each flow channel and chamber on the detection plate is in a sealed state; the worker injects the pretreated nucleic acid sample into the dispersion flow channel from the injection port, then seals the injection port, and then starts the power source; the nucleic acid sample is pushed into the primary chamber along the dispersion flow channel by the power source; then the power source is controlled in a positive and negative manner, and the nucleic acid sample in the primary chamber is uniformly mixed with the primer probe lyophilized powder, the PH buffer, and the enzyme mixture by the positive and negative pressure output by the power source; the pressure stabilizing flow channel is arranged to balance the air pressure before and after the primary chamber, so as to avoid driving the mixed nucleic acid sample in the primary chamber downward when the power source outputs constantly; when the power source outputs pressurized or pulsed, the mixed nucleic acid sample is pushed to move downward; then the power source increases the output, and the mixed nucleic acid sample in the primary chamber flows into the secondary chamber along the communication channel; then the hierarchical release liquid detection apparatus is placed in a constant temperature incubator for incubation; and then the reaction positive and negative is judged according to the color change or turbidity change of the reaction system.

[0027] The present application provides a detection device including the hierarchical release liquid detection apparatus. The detection device has the advantages described above compared with the prior art, which will not be repeated here. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to make the technical solutions in the specific embodiments or prior art of the present application clearer, the accompanying drawings needed in the specific embodiments or prior art description will be briefly introduced as follows. Obviously, the accompanying drawings in the following description are some embodiments of the present application, and all other embodiments obtained by those of ordinary skill in the art without any creative work on the premise of the accompanying drawings also belong to the protection scope of the present application.

[0029] Figure 1 The first embodiment schematic diagram of the hierarchical release liquid detection device provided by the embodiment of the present application is shown in the figure.

[0030] Figure 2 The second embodiment schematic diagram of the hierarchical release liquid detection device provided by the embodiment of the present application is shown in the figure.

[0031] Icon:

[0032] 100 - detection plate; 110 - through hole;

[0033] 200 - power source;

[0034] 300 - dispersion flow channel; 301 - sample inlet; 310 - shunt flow channel; 320 - inlet flow channel; 330 - semicircular chamber;

[0035] 410 - first primary chamber; 420 - second primary chamber; 430 - third primary chamber;

[0036] 500 - pressure stabilizing flow channel;

[0037] 600 - secondary chamber; 610 - exhaust passage;

[0038] 710 - communication flow channel; 720 - three-way chamber. DETAILED DESCRIPTION

[0039] The technical solutions of the present application will be described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without any creative work on the premise belong to the protection scope of the present application.

[0040] In the description of the present application, it needs to be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0041] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise explicitly specified and limited.

[0042] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral; it can be mechanical connection, or electrical connection; it can be direct connection, or indirect connection through intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0043] The present application will be further described in detail below by specific embodiments and in conjunction with the drawings.

[0044] Referring to Figure 1 As shown in the drawings, the present embodiment provides a hierarchical release liquid detection device, which comprises a detection plate 100 and a power source 200; the detection plate 100 is provided with a dispersion flow channel 300, a primary chamber, a pressure stabilizing flow channel 500 and a secondary chamber 600; the power source 200 is connected with one end of the dispersion flow channel 300, and the other end of the dispersion flow channel 300 is a sample inlet 301; the dispersion flow channel 300 is connected with a plurality of primary chambers, and the plurality of primary chambers are connected with the secondary chamber 600 through a communication flow channel 710; the communication flow channel 710 is provided with a three-way chamber 720, and the primary chamber is connected with the three-way chamber 720 through the pressure stabilizing flow channel 500.

[0045] Specifically, when performing the detection work, the primer probe freeze-dried powder, the PH buffer and the enzyme mixture are mixed in the first chamber and the second chamber, and the sealing film is covered on the detection plate 100, so that each flow channel and the chamber on the detection plate 100 are in a sealed state. Then, the worker injects the pretreated nucleic acid sample into the dispersion flow channel 300 from the sample inlet 301, and then seals the sample inlet 301. Then, the power source 200 is started, and the nucleic acid sample is pushed into the first chamber along the dispersion flow channel 300 by the power source 200. Then, the power source 200 is controlled in positive and negative directions, and the nucleic acid sample in the first chamber is mixed uniformly with the primer probe freeze-dried powder, the PH buffer and the enzyme mixture and the like by the positive and negative pressure output by the power source 200. By arranging the pressure stabilizing flow channel 500, the air pressure before and after the first chamber can be balanced, so that the mixed nucleic acid sample in the first chamber is not driven downward when the power source 200 outputs constantly. When the power source 200 outputs pressurized or pulsed, the mixed nucleic acid sample can be pushed downward. Then, the mixed nucleic acid sample in the first chamber is flowed into the second chamber 600 along the communication channel by the increased output of the power source 200. Then, the hierarchical release liquid detection device is placed in the constant temperature incubator for incubation. Then, the reaction positive and negative results are judged according to the color change or turbidity change of the reaction system.

[0046] The second chamber 600 is provided with an exhaust channel 610.

[0047] The arrangement of the pressure stabilizing flow channel 500 can make the gas in the first chamber and the second chamber enter the second chamber 600 along the pressure stabilizing flow channel 500, and then be discharged from the exhaust channel 610 of the second chamber 600, so as to avoid that the gas pushes the mixed nucleic acid sample in the first chamber and the second chamber to move.

[0048] The power source 200 can adopt an air pump. In addition, those skilled in the art can also select the type of the power source 200 according to actual needs, which will not be described here.

[0049] Referring to Figure 1 In the optional solution of the embodiment, the dispersion flow channel 300 includes a branch flow channel 310 and an inlet flow channel 320. One end of the branch flow channel 310 is connected with the power source 200, and the other end is provided with the sample inlet 301. The plurality of first chambers are connected with the branch flow channel 310 through the inlet flow channel 320.

[0050] The dispersion flow channel 300 further includes a semicircular chamber 330, which is arranged on the branch flow channel 310 and connected with the branch flow channel 310 through the inlet flow channel 320.

[0051] The first-stage chambers include a first first-stage chamber 410, a second first-stage chamber 420, and a third first-stage chamber 430; the first first-stage chamber 410, the second first-stage chamber 420, and the third first-stage chamber 430 are connected with the shunt flow channel 310 through respective inlet flow channels 320. The first first-stage chamber 410, the second first-stage chamber 420, and the third first-stage chamber 430 are respectively connected with a pressure stabilizing flow channel 500.

[0052] Referring to Figure 1 In an optional embodiment of the present embodiment, the hierarchical release liquid detection device further includes an optical detection module and a light-emitting member; the light-emitting member is located above the second-stage chamber 600, and the optical detection module is located below the second-stage chamber 600.

[0053] The light-emitting member includes a fluorescent module, a natural light module, and / or a glow module.

[0054] Embodiment 1: Isothermal amplification reaction driven by a pressure pump

[0055] Referring to Figure 1 As shown in the hierarchical release liquid detection device provided in the present embodiment, a surface film is provided to tightly fit the chambers and flow channels on the surface of the detection plate 100. The power source 200 is a pressure pump or an air pump; the first first-stage chamber 410 is preloaded with primer probe freeze-dried powder, the second first-stage chamber 420 is preloaded with pH buffer, and the third first-stage chamber 430 is preloaded with enzyme mixture.

[0056] The hierarchical release liquid detection device provided in the present embodiment is used to perform isothermal amplification reaction. A pretreated nucleic acid sample is injected into the shunt flow channel 310 through the sample inlet 301, and then the sample is sealed by plugging. The pressure pump is started, and the driving force drives the sample to flow into the first-stage chambers through the inlet flow channels 320. The sample liquid enters the first-stage chambers and mixes and dissolves with the pre-packaged reagents therein. By operating the power source 200, the positive and negative driving forces are reversed to mix and homogenize the liquid in each first-stage chamber. Further, the pressure pump drives the liquid in each first-stage chamber to flow through the communication flow channel 710 and the three-way chamber 720, and finally into the second-stage chamber 600. Then, the hierarchical release liquid detection device is placed in a constant-temperature incubator for incubation for 30 minutes. The reaction is judged by naked eye according to the color change or turbidity change of the reaction system.

[0057] Embodiment 2: Disc isothermal amplification reaction driven by centrifugal force

[0058] Referring to Figure 2As shown in the figure, a film is coated on the surface of the detection plate 100, tightly adhering to the surface of the detection plate 100, each chamber and the flow channel, and the detection plate 100 adopts a disc. A through hole 110 is arranged at the center of the disc, and the detection plate 100 is connected with the centrifugal motor through the through hole 110. The first primary chamber 410 is preloaded with primer probe freeze-dried powder, the second primary chamber 420 is preloaded with pH buffer, and the third primary chamber 430 is preloaded with enzyme mixture.

[0059] The isothermal amplification reaction is carried out using the hierarchical release liquid detection device. The pretreated nucleic acid sample is injected into the shunt flow channel 310 through the sample inlet 301, and then the sample inlet 301 is sealed by plugging. The centrifugal motor is started, and the centrifugal motor rotates to generate centrifugal force to drive the liquid to flow through the inlet flow channel 320 to the primary chamber. The sample liquid enters the primary chamber and mixes and dissolves with the reagent pre-packaged therein. By controlling the forward and reverse rotation of the centrifugal motor, the liquid in each primary chamber is uniformly mixed. Further, the centrifugal motor increases the speed to drive the liquid in each primary chamber to flow through the communication flow channel 710 and the three-way chamber 720, and finally enters the secondary chamber 600. Then the hierarchical release liquid detection device is placed in a constant temperature incubator for incubation for 30 minutes. The reaction positive and negative are judged by naked eye according to the color change or turbidity change of the reaction system.

[0060] Example 3: Pressure pump driven fluorescence isothermal amplification reaction

[0061] Referring to Figure 1 or Figure 2 As shown in the figure, a film is coated on the surface of the detection plate 100, tightly adhering to the surface of the detection plate 100, each chamber and the flow channel, and the detection plate 100 adopts a disc. A through hole 110 is arranged at the center of the disc, and the detection plate 100 is connected with the centrifugal motor through the through hole 110. The first primary chamber 410 is preloaded with primer probe freeze-dried powder, the second primary chamber 420 is preloaded with pH buffer, and the third primary chamber 430 is preloaded with enzyme mixture.

[0062] Using the structure for isothermal amplification reaction, taking the pretreated nucleic acid sample, injecting into the shunt flow channel 310 through the sample inlet 301, then plugging the sample inlet 301. Start the pressure pump, the driving force drives the sample to flow through the inlet flow channel 320 to the first chamber. The sample liquid enters the first chamber, mixes and dissolves with the reagent pre-packaged therein. By manipulating the power source 200, the positive and negative driving forces are mixed to uniformly mix the liquid in each first chamber. Further, start the optical detection module, read the absorbance of each liquid in the first chamber compared with the system preset value, judge whether the liquid is deteriorated. Then, the pressure pump drives the liquid in each first chamber to flow through the communication flow channel 710 and the three-way chamber 720, and finally enters the second chamber 600. Then put the hierarchical release liquid detection device into the incubator for incubation for 30 minutes. The optical module reports the fluorescence signal in the second chamber 60018, which can judge the positive and negative of the reaction structure.

[0063] Example 4: pressure pump driven multiple fluorescence isothermal amplification reaction

[0064] Referring to Figure 1 or Figure 2 As shown, the surface of the detection plate 100 is covered with a film, which tightly detects the surface of the detection plate 100, each chamber and flow channel, to form a sealed environment. The power source 200 uses a pressure pump or an air pump; the first first chamber 410 is preloaded with deoxyribonucleotide freeze-dried powder, the second first chamber 420 is preloaded with pH buffer, and the third first chamber 430 is preloaded with enzyme mixture; further comprising a third chamber, the third chamber is connected with the second chamber 600, the first chamber and the third chamber are provided with a fluorescence detection module above, the fluorescence detection module includes an excitation light module and an emission light receiving module. The third chamber includes a third first chamber, a third second chamber and a third third chamber.

[0065] Using the structure to carry out isothermal amplification reaction, take the pretreatment of nucleic acid sample, through the injection port 301 injection shunt flow channel 310, then the injection port 301 plug seal. Start pressure pump, driving force drives the sample through the inlet flow channel 320 to the first chamber flow. Sample liquid into the first chamber, mixed with the reagent pre packaged in it and dissolved. By manipulating the power source 200, positive and negative driving force mixed evenly each first chamber liquid. Further, start the optical detection module, read the first chamber liquid absorbance compared with the system preset value, determine whether the liquid is deteriorated. Then, the pressure pump drives the liquid in each first chamber flow through the communication flow channel 710 and three way chamber 720, finally into the second chamber 600 mixed evenly. Finally, start the pressure pump again, drive the liquid through the flow channel into the third chamber, mixed liquid into each third chamber to fully dissolve the various primer probe freeze-dried powder in it. The hierarchical release liquid detection device is put into the incubator for incubation for 30 minutes. The optical module reports the different fluorescence signals in each chamber of the third chamber, realizes multi target detection, that is, judges the positive and negative of multiple targets.

[0066] The embodiment provides a detection device, comprising the hierarchical release liquid detection device.

[0067] Specifically, the detection device provided by the embodiment has the advantages of the hierarchical release liquid detection device compared with the prior art, and details are not described herein.

[0068] Finally, it should be noted that: the above examples are used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A staged release liquid detection device, characterized in that, include: Detection plate (100) and power source (200); The detection plate (100) is provided with a dispersion channel (300), a primary chamber, a pressure stabilizing channel (500) and a secondary chamber (600). The power source (200) is connected to one end of the dispersion channel (300), and the other end of the dispersion channel (300) is the sample inlet (301). The dispersion channel (300) is connected to multiple primary chambers, and the multiple primary chambers are connected to the secondary chamber (600) through a connecting channel (710). A three-way chamber (720) is provided on the connecting channel (710), and the primary chambers are connected to the three-way chamber (720) through a pressure stabilizing channel (500). By setting up the pressure stabilizing channel (500), the air pressure before and after the primary chamber can be balanced, preventing the sample in the primary chamber from being driven downward when the power source (200) is outputting at a constant speed.

2. The staged release liquid detection device according to claim 1, characterized in that, The dispersion channel (300) includes a branch channel (310) and an inlet channel (320); One end of the diversion channel (310) is connected to the power source (200), and the other end is provided with the sample inlet (301). The multiple primary chambers are connected to the branch flow channel (310) via the inlet flow channel (320).

3. The staged release liquid detection device according to claim 2, characterized in that, The dispersion channel (300) further includes a semi-circular chamber (330), which is disposed on the diversion channel (310), and the inlet channel (320) is connected to the diversion channel (310) through the semi-circular chamber (330).

4. The staged release liquid detection device according to claim 3, characterized in that, The primary chamber includes a first primary chamber (410), a second primary chamber (420), and a third primary chamber (430). The first-stage chamber (410), the second-stage chamber (420) and the third-stage chamber (430) are all connected to the branch flow channel (310) through their respective inlet flow channels (320).

5. The staged release liquid detection device according to claim 4, characterized in that, The first-stage chamber (410), the second-stage chamber (420) and the third-stage chamber (430) are all connected to the pressure-stabilizing flow channel (500).

6. The staged release liquid detection device according to claim 5, characterized in that, The secondary chamber (600) is provided with an exhaust channel (610).

7. The staged release liquid detection device according to any one of claims 1-6, characterized in that, It also includes an optical detection module and a light-emitting component; The light-emitting element is located above the secondary chamber (600), and the optical detection module is located below the secondary chamber (600).

8. The staged release liquid detection device according to claim 7, characterized in that, The light-emitting element includes a fluorescent module, a natural light module, and / or a glow module.

9. The staged release liquid detection device according to any one of claims 1-6, characterized in that, The power source (200) is an air pump.

10. A testing device, characterized in that, Includes the graded release liquid detection device according to any one of claims 1-9.

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