Liquid chromatography system and cleaning method thereof, and computer readable storage medium

Abstract

The present invention provides a liquid chromatography system and a cleaning method thereof and a computer-readable storage medium. In the liquid chromatography system, a processor is configured to use a first flow path, which is a flow path for analysis including a first column, to analyze a sample, obtain at least one of an analysis result for quality management of the first flow path and information for determining an analysis target sample in the first flow path, determine a first combination of execution conditions corresponding to the analysis result for quality management and at least one of the information from two or more combinations of cleaning methods and cleaning execution conditions, and drive one or more cleaning pumps according to the method included in the first combination.

Description

Technical Field

[0001] The present disclosure relates to a liquid chromatography system and a cleaning method thereof, and a computer-readable storage medium. Background Art

[0002] Liquid chromatography is a technique for separating components contained in a sample by introducing a sample to be analyzed together with an eluent as a mobile phase into a column. The components of the sample separated by liquid chromatography are sometimes analyzed by a mass spectrometer based on the properties of the components.

[0003] International Publication No. 2017 / 216934 describes a chromatography mass spectrometer including a plurality of streams for liquid chromatography for the purpose of improving the processing capacity of analysis. The chromatography mass spectrometer described in International Publication No. 2017 / 216934 has three streams connected to a column. The chromatography mass spectrometer described in International Publication No. 2017 / 216934 connects any one of the three streams to the mass analyzer through a switching valve connected to the mass analyzer.

[0004] In the liquid chromatography system of the chromatography mass spectrometer, a part of the sample used in the analysis until the last time is sometimes accumulated as dirt in the needle in the automatic sampler or the valve, column, etc. in the flow path. The accumulation of such dirt is also called carryover, and the analysis until the last time will be carried over to this analysis, so that the peaks from the sample that was not originally the object of measurement will be detected in the mass spectrometer. Therefore, in the liquid chromatography system, the flow path is cleaned after each analysis. However, in the past, the conditions during cleaning were different according to the properties of the sample as the object of measurement or the type of column or piping used in the flow path, so it was difficult to avoid the generation of carryover or to reduce and eliminate it in a short time when carryover was generated only by specific cleaning conditions. Summary of the invention

[0005] An object of the present disclosure is to provide a technique for properly cleaning a flow path in a liquid chromatography system.

[0006] According to one aspect of the present disclosure, a liquid chromatography system includes: a first column for separating a sample into each component; a first flow path, which is a flow path for analysis including the first column; one or more cleaning pumps for supplying cleaning liquid to the first flow path; a memory for storing two or more combinations of cleaning methods and cleaning execution conditions; and a processor, the processor being configured to use the first flow path to analyze the sample, obtain an analysis result for quality management of the first flow path and determine at least one of the information of the analysis object sample in the first flow path, determine a first combination of execution conditions corresponding to at least one of the analysis result and information for quality management from the two or more combinations, and drive the one or more cleaning pumps according to the method contained in the first combination.

[0007] According to another aspect of the present disclosure, a liquid chromatography system includes: a first flow path, including a flow path for analysis; one or more cleaning pumps, supplying cleaning liquid to the first flow path; a processor; and a memory, storing one or more analysis conditions and one or more cleaning methods, in which the one or more analysis conditions are respectively combined with any one of the one or more cleaning methods, and the processor decides to use the first flow path for analysis of the sample, and after analysis based on any one of the one or more analysis conditions, drives one or more cleaning pumps based on a cleaning method of the one or more cleaning methods combined with an analysis condition.

[0008] A cleaning method according to one aspect of the present disclosure is a cleaning method for a liquid chromatography system, wherein the liquid chromatography system comprises: a first flow path, comprising an analysis flow path having a first analysis column; one or more pumps, supplying liquid to the first flow path; and a memory, storing two or more combinations of cleaning methods and cleaning execution conditions, the cleaning method comprising the following steps: determining to use the first flow path for sample analysis; corresponding to a situation in which it has been determined to use the first flow path for sample analysis, obtaining at least one of an analysis result for quality management of the first flow path and information determining the analysis object sample in the first flow path; determining, from the two or more combinations, a first combination of execution conditions corresponding to at least one of the analysis result for quality management and the information; and driving the one or more pumps according to the method contained in the first combination.

[0009] A computer-readable storage medium according to one aspect of the present disclosure is a non-temporary computer-readable storage medium having a program recorded thereon, wherein the program is executed by a processor of a controller to cause the controller to implement the following steps: in a liquid chromatography system, determining to use a first flow path including an analysis flow path having a first analysis column for sample analysis; in response to the decision to use the first flow path for sample analysis, obtaining at least one of an analysis result for quality management of the first flow path and information identifying the sample to be analyzed in the first flow path; determining a first combination of execution conditions corresponding to at least one of the analysis result for quality management and the information from two or more combinations of cleaning methods and cleaning execution conditions; and driving one or more pumps for supplying liquid to the first flow path according to the method contained in the first combination.

[0010] According to the present disclosure, the flow path can be properly cleaned in a liquid chromatography system. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a schematic diagram of the structure of a liquid chromatography system.

[0012] Figure 2 It is a diagram showing the structure of a liquid chromatography system.

[0013] Figure 3 It is a diagram showing the structure of a liquid chromatography system.

[0014] Figure 4 is a block diagram showing the structure of a liquid chromatography system.

[0015] Figure 5 This is a diagram illustrating a state where a sample is sucked through a needle.

[0016] Figure 6 This is a diagram illustrating a state in which a sample sucked by a needle is injected into the injection port.

[0017] Figure 7 This is a diagram illustrating a state in which, after a sample is introduced into a column, an eluent is injected into the column.

[0018] Figure 8 This is a diagram illustrating a state in which a flow path to be analyzed is switched from a first flow path to a second flow path.

[0019] Fig. 9 It is a diagram showing a comparative example with respect to the liquid chromatography system according to the present embodiment.

[0020] Fig.10 It is a diagram for explaining the outline of the first cleaning mode to the fifth cleaning mode.

[0021] Fig.11 It is a diagram showing a specific configuration example of the first cleaning mode.

[0022] Fig.12 It is a diagram showing a specific configuration example of the second cleaning mode.

[0023] Fig.13 It is a diagram showing a specific configuration example of the third cleaning mode.

[0024] Fig.14 It is a diagram showing a specific configuration example of the fourth cleaning mode.

[0025] Fig.15 It is a diagram showing a specific configuration example of the fifth cleaning mode.

[0026] Fig.16 This is a diagram showing an example in which the flow channel is cleaned by the third cleaning mode and the fourth cleaning mode during the suction of the sample.

[0027] Fig.17 FIG. 1 is a diagram showing an example in which the flow channel is cleaned in the fourth cleaning mode during injection of a sample.

[0028] Fig.18 This is a diagram showing an example of cleaning the flow channel in the second cleaning mode during sample analysis.

[0029] Fig.19 This is a diagram showing an example of cleaning the flow channel by the fourth cleaning mode and the fifth cleaning mode during the analysis of the sample.

[0030] Fig. 20 It is a diagram showing the cleaning modes selectable in the first to fourth analysis flow paths.

[0031] Fig.21 This is a timing chart showing an example of the setting of the cleaning mode.

[0032] Fig. 22 2 is a timing chart showing an example of a driving pattern of the washer pump and the high-pressure pump.

[0033] Fig.23 This is a flowchart of a process for accepting input of settings related to analysis from a user in the liquid chromatography system 10 .

[0034] Fig.24 This is a diagram showing an example of a setting screen.

[0035] Fig.25 This is a diagram schematically showing an example of the data structure of the method file database.

[0036] Fig.26 1 is a flowchart of a process for analyzing a sample in the liquid chromatography system 10 .

[0037] Fig. 27This is a diagram showing an example of a screen displaying flow path information.

[0038] Fig.28 It is a diagram showing a first modified example of the method file database.

[0039] Fig.29 It is a diagram showing a second modified example of the method file database. DETAILED DESCRIPTION

[0040] Hereinafter, the embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. In addition, the same or corresponding parts in the drawings are denoted by the same reference numerals, and their description will not be repeated.

[0041] <General structure>

[0042] Figure 1 1 is a schematic diagram of the liquid chromatography system 10. In the liquid chromatography system 10, flow paths 291A to 291D used for sample analysis are configured. The flow paths 291A to 291D are configured to include high pressure valves 180A to 180D, respectively. The flow paths 291A to 291D are connected to a flow path 292 toward a detector 500.

[0043] A divert valve 90 is disposed between the flow paths 291A to 291D and the flow path 292 .

[0044] The flow paths 291A to 291D are respectively switched to a first flow path that goes to the diverter valve 90 via the sample injection device 100 and a second flow path that goes to the diverter valve 90 without going through the sample injection device 100. The sample injection device 100 includes a needle for injecting a sample.

[0045] The diverter valve 90 includes ports 91 to 97. Port 91 is connected to a flow path 291A. Port 92 is connected to a flow path 291B. Port 93 is connected to a flow path 291C. Port 94 is connected to a flow path 291D. Port 95 is connected to a detector 500. Ports 96 and 97 are connected to a drain pipe (not shown). Port 95 is equivalent to a main port. Ports 96 and 97 are equivalent to drain pipe ports.

[0046] The flow dividing valve 90 constitutes a switching valve that switches the connection destination of the port 95 to any one of the ports 91 to 94. The flow dividing valve 90 connects any one of the flow paths 291A to 291D to the flow path 292 that leads to the detector 500.

[0047] The structure of flow path 291A will be described in detail.

[0048] The flow path 291A includes the high pressure valve 180A and extends from the high pressure valve 180A toward the column 230A. The flow path 291A is switched by the high pressure valve 180A to a first flow path toward the column 230A via the sample injection device 100 and a second flow path toward the column 230A without passing through the sample injection device 100.

[0049] In the flow path 291A, at least a high pressure pump 220A, a cleaning pump 143A, a cleaning valve 18A, a high pressure valve 180A, and a column 230A are arranged. The high pressure valve 180A is connected to the column 230A. The column 230A is filled with a stationary phase for separating components of a sample.

[0050] The high-pressure valve 180A is connected to the high-pressure pump 220A and the cleaning pump 143A via the cleaning valve 18A. The high-pressure pump 220A supplies the eluent loaded in the container 210A to the high-pressure valve 180A. The cleaning pump 143A supplies the rinse liquid loaded in the container 250A to the high-pressure valve 180A. The cleaning valve 18A connects either the high-pressure pump 220A or the cleaning pump 143A to the high-pressure valve 180A. As a result, the eluent or the rinse liquid is supplied to the high-pressure valve 180A.

[0051] When the flow path 291A is set as the first flow path through the sample injection device 100, the eluent supplied from the high pressure pump 220A to the high pressure valve 180A flows to the column 230A through the sample injection device 100. The sample held by the sample injection device 100 is sent to the column 230A via the eluent.

[0052] When the flow path 291A is set as the second flow path that does not pass through the sample injection device 100, the eluent supplied from the high-pressure pump 220A to the high-pressure valve 180A flows to the column 230A without passing through the sample injection device 100. When the injection of the sample into the column 230A is completed, the eluent is sent from the high-pressure valve 180A to the column 230A through the second flow path. Thus, the sample is separated in the column 230A.

[0053] The column 230A is connected to the port 91 of the diverter valve 90. When the diverter valve 90 connects the port 91 and the port 95, the components of the sample separated in the column 230A flow to the detector 500 through the diverter valve 90. As a result, the components of the sample separated in the column 230A are analyzed by the detector 500 including a mass analyzer and the like.

[0054] When the cleaning pump 143A and the high-pressure valve 180A are connected via the cleaning valve 18A, the cleaning liquid is supplied to the high-pressure valve 180A. The high-pressure valve 180A allows the cleaning liquid to flow to the column 230A via the sample injection device 100 or without passing through the sample injection device 100. Thus, both the first flow path to the column 230A via the sample injection device 100 and the second flow path to the column 230A without passing through the sample injection device 100 can be cleaned.

[0055] When the port 91 of the diverter valve 90 is connected to the port 95, the flushing liquid flows from the column 230A through the port 91 and the port 95 of the diverter valve 90 toward the detector 500. As a result, the flow path 292 from the diverter valve 90 to the detector 500 is also cleaned. When the port 91 of the diverter valve 90 is connected to the port 96 and the port 97, the port 91 of the diverter valve and the ports 96 and the port 97 are cleaned.

[0056] The structure of the flow path 291A has been described in detail above. Next, the structures of the flow paths 291B to 291D will be described.

[0057] The flow path 291B includes the high pressure valve 180B and extends from the high pressure valve 180B to the column 230B. The flow path 291B is switched by the high pressure valve 180B to a first flow path extending to the column 230B via the sample injection device 100 and a second flow path extending to the column 230B without passing through the sample injection device 100.

[0058] In the flow path 291B, at least a high-pressure pump 220B for sucking an eluent from the container 210B, a cleaning pump 143B for sucking a rinse liquid from the container 250B, a cleaning valve 18B, a high-pressure valve 180B, and a column 230B are arranged.

[0059] The flow path 291C includes the high pressure valve 180C and extends from the high pressure valve 180C toward the column 230C. The flow path 291C is switched by the high pressure valve 180C into a first flow path toward the column 230C via the sample injection device 100 and a second flow path toward the column 230C without passing through the sample injection device 100.

[0060] In the flow path 291C, at least a high-pressure pump 220C for sucking up an eluent from the container 210C, a cleaning pump 143C for sucking up a rinse liquid from the container 250C, a cleaning valve 18C, a high-pressure valve 180C, and a column 230C are arranged.

[0061] The flow path 291D includes the high pressure valve 180D and extends from the high pressure valve 180D toward the column 230D. The flow path 291D is switched by the high pressure valve 180D into a first flow path toward the column 230D via the sample injection device 100 and a second flow path toward the column 230D without passing through the sample injection device 100.

[0062] In the flow path 291D, at least a high-pressure pump 220D for sucking an eluent from the container 210D, a cleaning pump 143D for sucking a rinse liquid from the container 250D, a cleaning valve 18D, a high-pressure valve 180D, and a column 230D are arranged.

[0063] Thus, the configurations of the flow paths 291B to 291D are the same as that of the flow path 291A. Therefore, the detailed description of the flow path 291A will be used as the detailed description of the flow paths 291B to 291D.

[0064] Hereinafter, the flow path 291A, the flow path 291B, the flow path 291C, and the flow path 291D are also referred to as the first analysis flow path 291A, the second analysis flow path 291B, the third analysis flow path 291C, and the fourth analysis flow path 291D, respectively. The first analysis flow path 291A, the second analysis flow path 291B, the third analysis flow path 291C, and the fourth analysis flow path 291D are respectively switched to a first flow path toward the diverter valve 90 via the sample injection device 100 and a second flow path toward the diverter valve 90 without passing through the sample injection device 100.

[0065] The liquid chromatography system 10 can switch the flow path used for analysis between the first analysis flow path 291A, the second analysis flow path 291B, the third analysis flow path 291C, and the fourth analysis flow path 291D. Therefore, according to the liquid chromatography system 10, various samples can be continuously analyzed in the detector 500. As a result, according to the liquid chromatography system 10, the analysis efficiency can be improved.

[0066] Furthermore, the liquid chromatography system 10 includes a cleaning pump 143A corresponding to the first analysis channel 291A, a cleaning pump 143B corresponding to the second analysis channel 291B, a cleaning pump 143C corresponding to the third analysis channel 291C, and a cleaning pump 143D corresponding to the fourth analysis channel 291D. These structures can clean the channels in various modes in the liquid chromatography system 10.

[0067] For example, when the first analysis channel 291A is used for sample analysis, a desired channel among the second analysis channel 291B, the third analysis channel 291C, and the fourth analysis channel 291D can be cleaned.

[0068] <Structure of liquid chromatography system 10>

[0069] Figure 2 as well as Figure 3 is a diagram showing the structure of the liquid chromatography system 10. In particular, Figure 3 2 shows the structure of the diverter valve 90 included in the liquid chromatography system 10.

[0070] If used Figure 1 As illustrated, the liquid chromatography system 10 includes four high pressure valves 180A-180D. Figure 2 In the Figure 1 A diagram showing the structure associated with the high-pressure valve 180C among the four high-pressure valves 180A to 180D shown.

[0071] The high-pressure valves 180A to 180D are connected to the first switching valve 150 and the second switching valve 160. The first switching valve 150 and the second switching valve 160 have a function of selecting a high-pressure valve related to the suction and injection of the sample among the high-pressure valves 180A to 180D. The first switching valve 150 and the second switching valve 160 include, for example, multi-way switching valves.

[0072] The first switching valve 150 is connected to a needle valve 260. The needle valve 260 is connected to a needle 191 via a sample loop 192. The needle 191 is a needle-shaped part for sucking a sample. The sample loop 192 holds the sample sucked by the needle 191. The needle moving mechanism 190 moves the needle 191 in three orthogonal axial directions.

[0073] The liquid chromatography system 10 includes injection ports 198A to 198D. The injection port 198A is provided corresponding to the high pressure valve 180A. The injection port 198B is provided corresponding to the high pressure valve 180B. The injection port 198C is provided corresponding to the high pressure valve 180C. The injection port 198D is provided corresponding to the high pressure valve 180D.

[0074] Containers 302A to 302C containing samples are placed on the sample stage 300. The needle moving mechanism 190 moves the needle 191 to suck the sample from any one of the containers 302A to 302C. The needle moving mechanism 190 moves the needle 191 to inject the sucked sample into any one of the injection ports 198A to 198D.

[0075] The needle valve 260 is further connected to the needle cleaning pump 20 .

[0076] The second switching valve 160 is connected to the low pressure valve 170. The low pressure valve 170 is connected to the metering pump 130. The metering pump 130 is used to draw a predetermined amount of a sample using the needle 191.

[0077] The high pressure valve 180A includes ports 181A to 186A. Port 181A is connected to a drain pipe not shown. That is, port 181A is a drain pipe port. Port 182A is connected to the injection port 198A. Port 183A is connected to the column 230A. Port 184A is connected to the high pressure pump 220A and the cleaning pump 143A via the cleaning valve 18A. Port 185A is connected to the first switching valve 150. Port 186A is connected to the second switching valve 160.

[0078] The high pressure valve 180A includes connecting portions 187A to 189A. The connecting portions 187A to 189A switch the connection state of the ports 181A to 186A between the first state and the second state.

[0079] The first state is Figure 2 That is, the first state is a state in which the port 181A is connected to the port 182A, the port 183A is connected to the port 184A, and the port 185A is connected to the port 186A.

[0080] In the first state, the first switching valve 150 and the second switching valve 160 are connected via the high pressure valve 180A. In the first state, the column 230A and the high pressure pump 220A or the cleaning pump 143A are connected via the high pressure valve 180A. In the first state, the injection port 198A is connected to the port 181A which is the discharge port of the high pressure valve 180A.

[0081] The second state is Figure 1 The connection parts 187A to 189A shown in the figure are rotated 30 degrees with the center of the high pressure valve 180A as the axis. That is, the second state is a state in which the port 182A is connected to the port 183A, the port 184A is connected to the port 185A, and the port 186A is connected to the port 181A. The second state is, for example, Figure 6 shown.

[0082] The high pressure valves 180B to 180D each include the same structure as the high pressure valve 180A. The high pressure valves 180B to 180D each switch between the first state and the second state in the same manner as the high pressure valve 180A. Further descriptions related to the high pressure valves 180B to 180D will substantially repeat the structural description of the high pressure valve 180A. Therefore, further descriptions of the high pressure valves 180A to 180D will not be repeated here.

[0083] The first switching valve 150 includes ports 151 to 155 . A high pressure valve 180A is connected to the port 151 . A high pressure valve 180B is connected to the port 152 . A high pressure valve 180C is connected to the port 153 . A high pressure valve 180D is connected to the port 154 . A needle valve 260 is connected to the port 155 .

[0084] The first switching valve 150 includes a connection portion 158. The connection portion 158 switches a connection destination to the port 155 among the ports 151 to 154.

[0085] The needle valve 260 includes ports 261 to 266 and connecting portions 267 to 269. The first switching valve 150 is connected to the port 261. The sample loop 192 is connected to the port 262. The needle cleaning pump 20 is connected to the port 263.

[0086] The needle valve 260 switches the state of the connection parts 267 to 269 to Figure 2 The state shown, and the connection parts 267 to 269 from Figure 2 The state shown is a state in which the needle valve 260 is rotated 30 degrees about the center of the needle valve 260.

[0087] Figure 2 In the state shown, the needle 191 is connected to the needle valve 260 via the sample loop 192, the needle valve 260 is connected to the first switching valve 150, and the first switching valve 150 is connected to the high-pressure valve 180A. Furthermore, the high-pressure valve 180A is connected to the second switching valve 160, and the second switching valve 160 is connected to the metering pump 130 via the low-pressure valve 170. Therefore, after the needle 191 is moved to any one of the containers 302A to 302C, the metering pump 130 is driven, thereby sucking the sample through the needle 191.

[0088] like Figure 3 As shown, the pipe columns 230A to 230D are connected to the diverter valve 90 . Figure 3 9 shows a state where port 95 formed in the center of diverter valve 90 is connected to port 91 corresponding to column 230A. At this time, ports 92 to 94 of diverter valve 90 are connected to ports 96 and 97 which are drain ports of diverter valve 90.

[0089] In this state, the flow path including the column 230A is connected to the detector 500. The sample of the column 230A can be analyzed in the detector 500. The flow path including the column 230B passes through the ports 96 and 97 of the diverter valve 90 and is directed to a drain pipe (not shown). The flow path including the column 230C and the flow path including the column 230D also pass through the ports 96 and 97 of the diverter valve 90 and are directed to a drain pipe (not shown).

[0090] As described above, the liquid chromatography system 10 includes a plurality of valves. In relation to the first switching valve 150 and the second switching valve 160, the diverter valve 90 may also be referred to as a third switching valve, and the needle valve 260 may also be referred to as a fourth switching valve.

[0091] <Block Diagram of Liquid Chromatography System 10>

[0092] Figure 4 2 is a block diagram showing the configuration of the liquid chromatography system 10. As described so far, the liquid chromatography system 10 includes a plurality of valves and a plurality of pumps.

[0093] The valves included in the liquid chromatography system 10 include a low-pressure valve 170 , high-pressure valves 180A to 180D, cleaning valves 18A to 18D, a needle valve 260 , a first switching valve 150 , a second switching valve 160 , and a diverter valve 90 .

[0094] Regarding the specific structure of these valves, Figure 1 to Figure 3 has been explained, so the description will not be repeated here.

[0095] The pumps included in the liquid chromatography system 10 include high pressure pumps 220A to 220D, cleaning pumps 143A to 143D, a needle cleaning pump 20, and a metering pump 130. The high pressure pumps 220A to 220D pump eluents from containers 210A to 210D, respectively. The cleaning pumps 143A to 143D pump flushing liquids from containers 250A to 250D, respectively.

[0096] The containers 210A to 210D may be filled with the same eluent or different eluents. The containers 250A to 250D may be filled with the same rinse solution or different rinse solutions.

[0097] The needle cleaning pump 20 draws the washing liquid from the container 200. The container 200 may contain the same washing liquid as the washing liquid contained in the containers 250A to 250D, or a washing liquid of a different type from the washing liquid contained in the containers 250A to 250D.

[0098] The sample injection device 100 includes a first switching valve 150 , a needle valve 260 , a needle 191 , and a sample loop 192 .

[0099] The liquid chromatography system 10 further includes a control device 110, an input device 120, a display device 125, and a needle moving mechanism 190. The needle moving mechanism 190 is described in detail. Figure 2 has been explained, so the description will not be repeated here.

[0100] The control device 110 includes a processor 111 and a memory 112. Typically, the processor 111 is a computing unit such as a central processing unit (CPU) or a multi-processing unit (MPU). The processor 111 reads out a program stored in the memory 112 and executes it, thereby realizing the processing of the liquid chromatography system 10.

[0101] The memory 112 is implemented by a non-volatile memory such as a random access memory (RAM), a read only memory (ROM), and a flash memory. As long as the memory 112 can non-temporarily record a program in a format readable by the processor 111, it may include a compact disc-read only memory (CD-ROM), a digital versatile disc-read only memory (DVD-ROM), a universal serial bus (USB) memory, a memory card, a flexible disk (FD), a hard disk, a solid state drive (SSD), a magnetic tape, a cassette tape, a magnetic optical disc (MO), a mini disc (MD), an integrated circuit (IC) card (except a memory card), an optical card, a mask ROM, or an electrical programmable read only memory (EPROM).

[0102] The input device 120 includes, for example, a keyboard and a mouse. The user can input various instructions to the control device 110 by operating the input device 120. The display device 125 displays an image corresponding to a video signal output by the control device 110.

[0103] The display device 125 displays the first to fourth analysis channels 291A to 291D (see FIG. 1 ) provided in the liquid chromatography system 10. Figure 1 ) setting information. The user can set the analysis schedule using the first analysis flow path 291A to the fourth analysis flow path 291D while viewing the screen of the display device 125. The control device 110 performs the analysis based on the input schedule and cleans the first analysis flow path 291A to the fourth analysis flow path 291D.

[0104] <Suction of sample>

[0105] Figure 5 1 and 2 are diagrams illustrating a state in which a sample is sucked by the needle 191. Here, an example in which the needle 191 sucks the sample injected into the injection port 198A from the container 302A will be described.

[0106] The injection port 198A corresponds to the high pressure valve 180A among the high pressure valves 180A to 180D. Therefore, the first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180A. As shown in the figure, the first switching valve 150 is connected to the needle 191 via the needle valve 260 and the sample loop 192. The needle moving mechanism 190 guides the needle 191 to the container 302A. The second switching valve 160 is connected to the metering pump 130 via the low pressure valve 170.

[0107] The metering pump 130 applies a predetermined negative pressure to the needle 191 via the low pressure valve 170, the second switching valve 160, the first switching valve 150 and the needle valve 260. Thus, the needle 191 draws a predetermined amount of sample from the container 302A. The sample drawn by the needle 191 is held near the sample loop 192, for example.

[0108] Figure 5 , an example of sucking a sample through the high pressure valve 180A is shown. By switching the connection destinations of the first switching valve 150 and the second switching valve 160 between the high pressure valves 180B to 180D, the sample is sucked through each of the high pressure valves 180B to 180D.

[0109] The first switching valve 150 and the second switching valve 160 constitute a switching device that switches the high-pressure valves passing through the flow path from the metering pump 130 to the needle 191 among the high-pressure valves 180A to 180D.

[0110] <Injection of sample>

[0111] Figure 6 This is a diagram illustrating a state in which a sample sucked by the needle 191 is injected into the injection port 198A.

[0112] When the sample is injected into the injection port 198A, the connection parts 187A to 189A are connected from Figure 5 The state shown starts with a 30 degree rotation about the center of the high pressure valve 180A. The cleaning valve 18A connects the high pressure valve 180A and the high pressure pump 220A. Furthermore, the needle moving mechanism 190 moves the needle 191 to the injection port 198A.

[0113] As a result, a flow path is formed from the high pressure pump 220A to the column 230A via the high pressure valve 180A, the first switching valve 150, the needle 191, the injection port 198A, and the high pressure valve 180A. Figure 1 As described above, it corresponds to the first flow path in the first analysis flow path 291A, which passes through the sample injection device 100. At this time, the column 230A is connected to the detector 500 via the diverter valve 90. Figure 6 In the figure, the connection state between the column 230A and the diverter valve 90 is omitted. The connection state is, for example, Figure 3 shown.

[0114] By driving the high-pressure pump 220A in the state where the flow path is formed as described above, the eluent is supplied to the high-pressure valve 180A. The eluent supplied to the high-pressure valve 180A flows toward the needle 191 via the first switching valve 150 and the like. Thus, the sample held near the sample loop 192 is injected into the injection port 198A from the front end of the needle 191 together with the eluent. The injected sample is directed toward the column 230A together with the eluent.

[0115] Figure 6 , an example of injecting a sample into the injection port 198A corresponding to the high pressure valve 180A is shown. By switching the connection destination of the first switching valve 150 between the high pressure valves 180B to 180D, the sample is injected into the injection ports 198B to 198D corresponding to the high pressure valves 180B to 180D, respectively.

[0116] <Injection of eluent>

[0117] Figure 7 This is a diagram illustrating a state in which, after the sample is introduced into the column 230A, an eluent is injected into the column 230A.

[0118] After the sample is introduced into the column 230A, the connection parts 187A to 189A are connected from Figure 6 The state shown starts with a 30 degree rotation about the center of the high pressure valve 180A. As a result, the high pressure pump 220A is connected to the column 230A via the port 184A and the port 183A of the high pressure valve 180A.

[0119] If this flow path is used Figure 1 As described above, it corresponds to the second flow path in the first analysis flow path 291A that does not pass through the sample injection device 100. At this time, the column 230A is connected to the detector 500 via the diverter valve 90. This connection state is, for example, Figure 3 By supplying the eluent from the high pressure pump 220A to the high pressure valve 180A, the sample is separated in the column 230A.

[0120] At this time, the injection port 198A is connected to the port 181A which is the discharge port of the high pressure valve 180A. Figure 6 In the state shown, the needle valve 260 is rotated 30 degrees with the center thereof as the axis. As a result, the needle cleaning pump 20 is connected to the needle 191 via the needle valve 260 and the sample loop 192 .

[0121] Figure 7 , an example of injecting the eluent into the column 230A is shown. By driving the high-pressure pumps 220B to 220D corresponding to the high-pressure valves 180B to 180D, respectively, the eluent is similarly injected into the columns 230B to 230D.

[0122] <Switching the analysis flow path>

[0123] Figure 8 291A is a diagram illustrating a state in which the flow path used for analyzing a sample is switched from the first analysis flow path 291A to the second analysis flow path 291B. The concepts of the first analysis flow path 291A and the second analysis flow path 291B are as described using Figure 1 As described.

[0124] When the flow path used for analyzing the sample is switched from the first analysis flow path 291A to the second analysis flow path 291B, the states of the first switching valve 150 and the second switching valve 160 are changed. That is, the connection portion 158 of the first switching valve 150 switches the connection destination of the port 155 from the port 151 to the port 152. The connection portion 168 of the second switching valve 160 switches the connection destination of the port 167 from the port 161 to the port 162.

[0125] Thus, the first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180B. As shown in the figure, the first switching valve 150 is connected to the needle 191 via the needle valve 260 and the sample loop 192. The second switching valve 160 is connected to the metering pump 130 via the low pressure valve 170.

[0126] For example, after the needle 191 is moved to any one of the containers 302A to 302C containing the sample, the control device 110 drives the metering pump 130. Thus, the sample can be sucked through the high-pressure valve 180B by the needle 191. The injection port 198B corresponds to the high-pressure valve 180B among the high-pressure valves 180A to 180D. Therefore, by injecting the sample sucked by the needle 191 into the injection port 198B, the sample can be guided to the column 230B corresponding to the second analysis flow path 291B.

[0127] <Structure of Comparative Example>

[0128] Fig. 9 1 is a diagram showing a comparative example with respect to the liquid chromatography system 10 according to the present embodiment. The comparative example includes a plurality of high-pressure valves 1800A to 1800F, a first switching valve 1500 , a second switching valve 1600 , and a low-pressure valve 1700 .

[0129] The first switching valve 1500 and the second switching valve 1600 are linked to one of the high pressure valves 1800A to 1800F. The low pressure valve 1700 is connected to one of the high pressure valves 1800A to 1800F via the second switching valve 1600.

[0130] In the comparative example, the cleaning pumps corresponding to the high-pressure valves 1800A to 1800F are not provided, but the cleaning pump 1400 is provided corresponding to the low-pressure valve 1700. By driving the cleaning pump 1400, the flushing liquid is supplied to the second switching valve 1600 via the low-pressure valve 1700. In the comparative example, when the first switching valve 1500 and the second switching valve 1600 are connected to the high-pressure valve 1800A, the flow path formed by the high-pressure valve 1800A can be cleaned by driving the cleaning pump 1400.

[0131] However, when the first switching valve 1500 and the second switching valve 1600 are connected to the high-pressure valve 1800A, the flow path formed by the high-pressure valve 1800B cannot be cleaned. Similarly, when the first switching valve 1500 and the second switching valve 1600 are connected to the high-pressure valve 1800A, the flow paths formed by the high-pressure valves 1800C to 1800F cannot be cleaned.

[0132] When the first switching valve 1500 and the second switching valve 1600 are connected to the high-pressure valve 1800A, the flow path formed by the high-pressure valve 1800A may be used to analyze the sample. At this time, the various flow paths formed by the high-pressure valves 1800B to 1800F are not used for the analysis of the sample. However, in the comparative example, the supply target of the flushing liquid from the cleaning pump 1400 is limited to the connection target of the second switching valve 1600. Therefore, in the comparative example, when the second switching valve 1600 is connected to the high-pressure valve 1800A, the various flow paths formed by the high-pressure valves 1800B to 1800F cannot be cleaned.

[0133] In contrast, the liquid chromatography system 10 according to the present embodiment includes the cleaning pumps 143A to 143D corresponding to the high-pressure valves 180A to 180D, respectively. Therefore, according to the liquid chromatography system 10, regardless of which of the high-pressure valves 180A to 180D the second switching valve 160 is connected to, any flow path formed by including the high-pressure valves 180A to 180D can be cleaned with the flushing liquid.

[0134] <Overview of the First to Fifth Cleaning Modes>

[0135] Fig.10 1 is a diagram for explaining the outline of the first cleaning mode to the fifth cleaning mode. Fig.10 The cleaning mode will be described with reference to the flow path including the high pressure valve 180A. Fig.10 The first to fifth cleaning modes shown are used to clean the flow path including the high-pressure valve 180A.

[0136] In the first cleaning mode and the second cleaning mode, the flow path including the high pressure valve 180A is set as in the upper left frame. In addition, the solid arrow in the diverter valve 90 is the flow path set in the first cleaning mode, and the dotted arrow in the diverter valve 90 is the flow path set in the second cleaning mode.

[0137] In the first cleaning mode and the second cleaning mode, the flushing liquid supplied from the cleaning pump 143A to the high-pressure valve 180A flows in the order of the high-pressure valve 180A, the first switching valve 150, the needle valve 260, the sample loop 192, the needle 191, the high-pressure valve 180A, the column 230A and the diverter valve 90.

[0138] The cleaning flow path set in the first cleaning mode and the second cleaning mode corresponds to the first flow path passing through the needle valve 260, the sample loop 192, and the needle 191. The first flow path is, for example, one form of the first analysis flow path 291A.

[0139] In the first cleaning mode, port 91 of the diverter valve 90 is connected to port 95, so the flushing liquid flowing into the diverter valve 90 flows through ports 91 and 95, and cleans the flow path from the diverter valve 90 to the detector 500. In the second cleaning mode, port 91 of the diverter valve 90 is connected to ports 96 and 97, so the flushing liquid flowing into the diverter valve 90 cleans port 91 and is discharged from ports 96 and 97.

[0140] In the third cleaning mode and the fourth cleaning mode, the flow path including the high pressure valve 180A is set as in the lower left frame. In addition, the solid arrow in the diverter valve 90 is the flow path set in the third cleaning mode, and the dotted arrow in the diverter valve 90 is the flow path set in the fourth cleaning mode.

[0141] In the third cleaning mode and the fourth cleaning mode, the flushing liquid supplied from the cleaning pump 143A to the high-pressure valve 180A flows in the order of the high-pressure valve 180A, the column 230A, and the diverter valve 90 .

[0142] The cleaning flow path set in the third cleaning mode and the fourth cleaning mode corresponds to a second flow path that does not pass through the needle valve 260, the sample loop 192, and the needle 191. The second flow path is, for example, one form of the first analysis flow path 291A.

[0143] In the third cleaning mode, port 91 of the diverter valve 90 is connected to port 95, so the flushing liquid flowing into the diverter valve 90 flows through ports 91 and 95, and cleans the flow path from the diverter valve 90 to the detector 500. In the fourth cleaning mode, port 91 of the diverter valve 90 is connected to ports 96 and 97, so the flushing liquid flowing into the diverter valve 90 cleans port 91 and is discharged from ports 96 and 97.

[0144] In the fifth cleaning mode, the flow path including the high pressure valve 180A is set as shown in the right frame. In the fifth cleaning mode, the flushing liquid supplied from the needle cleaning pump 20 to the needle valve 260 flows in the order of the needle valve 260, the sample loop 192, the needle 191, and the high pressure valve 180A. The flushing liquid flowing into the high pressure valve 180A is discharged from the port 181A of the high pressure valve 180A.

[0145] Next, the specific configurations of the first to fifth cleaning modes will be described. Hereinafter, the configuration of a flow path including the high-pressure valve 180A will be described as a representative example.

[0146] <First cleaning mode and second cleaning mode>

[0147] Fig.11 It is a diagram showing a specific configuration example of the first cleaning mode. Fig.12 It is a diagram showing a specific configuration example of the second cleaning mode. Fig.11 as well as Fig.12 In the figure, part of the structure is omitted and the structure related to the diverter valve 90 is surrounded by a frame. Figures 13 to 19 Same here.

[0148] In the first cleaning mode, for example, Fig.11 The flow path shown. That is, the port 151 of the first switching valve 150 is connected to the port 155. The needle 191 is connected to the injection port 198A. In the high pressure valve 180A, the port 182A is connected to the port 183A, the port 184A is connected to the port 185A, and the port 186A is connected to the port 181A. In the diverter valve 90, the port 91 is connected to the port 95.

[0149] When the flushing liquid is supplied from the cleaning pump 143A to the high pressure valve 180A, the flow path including the high pressure valve 180A, the first switching valve 150, the needle valve 260, the sample loop 192, the needle 191, the injection port 198A, the high pressure valve 180A, the column 230A, and the diverter valve 90 is cleaned by the flushing liquid. Furthermore, the flow path toward the detector 500 is cleaned by the flushing liquid. At this time, a container of a blank sample such as a flushing liquid or an eluent may be prepared in advance on the sample stage 300, and the flushing liquid may be sucked by the needle 191 to perform cleaning in the first cleaning mode.

[0150] In the second cleaning mode, for example, Fig.12 In the second cleaning mode, the setting of the flow path of the diverter valve 90 is different from that in the first cleaning mode. That is, in the second cleaning mode, the port 91 of the diverter valve 90 is connected to the port 96 and the port 97. Therefore, in the second cleaning mode, the flow path from the port 91 of the diverter valve 90 toward the port 96 and the port 97 is cleaned. At this time, a container of a blank sample such as a flushing liquid or an eluent may be prepared in advance on the sample table 300, and the sample may be cleaned in the second cleaning mode after being sucked out by the needle 191.

[0151] <Third cleaning mode and fourth cleaning mode>

[0152] Fig.13 It is a diagram showing a specific configuration example of the third cleaning mode. Fig.14 It is a diagram showing a specific configuration example of the fourth cleaning mode.

[0153] In the third cleaning mode, for example, Fig.13 That is, port 181A of high pressure valve 180A is connected to port 182A, port 183A is connected to port 184A, and port 185A is connected to port 186A. In diverter valve 90, port 91 is connected to port 95.

[0154] The flushing liquid supplied from the flushing pump 143A to the high pressure valve 180A flows toward the column 230A instead of the needle 191. As a result, the diverter valve 90 and the flow path from the diverter valve 90 to the detector 500 are cleaned by the flushing liquid.

[0155] In the fourth cleaning mode, for example, Fig.14 In the fourth cleaning mode, the setting of the flow path of the diverter valve 90 is different from that in the third cleaning mode. That is, in the fourth cleaning mode, the port 91 of the diverter valve 90 is connected to the port 96 and the port 97. Therefore, in the fourth cleaning mode, the flow path from the port 91 of the diverter valve 90 to the port 96 and the port 97 is cleaned.

[0156] <Fifth cleaning mode>

[0157] Fig.15 It is a diagram showing a specific configuration example of the fifth cleaning mode.

[0158] In the fifth cleaning mode, for example, Fig.15 That is, port 262 of needle valve 260 is connected to port 263, port 264 is connected to port 265, and port 266 is connected to port 261. Port 181A of high-pressure valve 180A is connected to port 182A, port 183A is connected to port 184A, and port 185A is connected to port 186A.

[0159] When the flushing liquid is supplied from the needle cleaning pump 20 to the needle valve 260, the flow path including the sample loop 192, the needle 191, the injection port 198A and the high pressure valve 180A is cleaned by the flushing liquid. At this time, a container of a blank sample such as a flushing liquid or an eluent may be prepared in advance on the sample stage 300, and the flushing liquid may be sucked by the needle 191 to perform cleaning in the fifth cleaning mode.

[0160] As described above, according to the first cleaning mode and the second cleaning mode, not only the flow path from the column 230A to the diverter valve 90 but also the flow path including the needle 191 and the sample loop 192 can be cleaned.

[0161] In the third cleaning mode and the fourth cleaning mode, the range that can be cleaned is smaller than that in the first cleaning mode and the second cleaning mode. However, the third cleaning mode and the fourth cleaning mode do not include the needle 191 and the sample loop 192, which has the effect of increasing the variation of the cleaning method. That is, by making use of the third cleaning mode and the fourth cleaning mode, the flow path can be cleaned at the time of aspirating the sample using the needle 191 and the sample loop 192.

[0162] According to the first cleaning mode and the third cleaning mode, the flow path toward the detector 500 can be cleaned including the port 95 of the diverter valve 90. This cleaning mode is effective, for example, when a high-concentration sample is used for analysis, or in a structure that can switch multiple analysis flow paths (first analysis flow path 291A to fourth analysis flow path 291D) to continuously analyze, as in the liquid chromatography system 10 involved in this embodiment.

[0163] When multiple analysis channels are switched for continuous analysis, components of the sample may accumulate in the diverter valve 90 for switching the analysis channels. In particular, components of the sample may repeatedly accumulate in the port 95 of the diverter valve 90 connected to the detector 500, thereby generating carryover. Alternatively, since the sample is continuously fed to the interface portion of the detector 500 through the diverter valve 90, carryover may be generated in the interface portion.

[0164] According to the first cleaning mode and the third cleaning mode, the port 95 of the diverter valve 90 and the interface part of the detector 500 can be included in the cleaning, so that efficient analysis using multiple analysis flow paths can be performed and the parts that become the cause of the carryover can be fully cleaned.

[0165] In use Figure 10 to Figure 15 In the description, an example of using a flushing liquid to clean the flow path is described. However, in the first cleaning mode to the fifth cleaning mode, an eluent (blank liquid) can also be used to clean the flow path. For example, in the first cleaning mode to the fourth cleaning mode, by replacing the cleaning pump 143A with the high-pressure pump 220A, cleaning using an eluent can also be achieved, and in the fifth cleaning mode, by connecting the needle cleaning pump 20 to a container filled with an eluent, cleaning using an eluent can also be achieved. Furthermore, in the first cleaning mode to the fifth cleaning mode, cleaning by combining a flushing liquid and an eluent can also be achieved. For example, after the flow path is cleaned with a flushing liquid, the flow path can be cleaned with an eluent.

[0166] Here, the first cleaning mode to the fifth cleaning mode are described by taking the flow path including the high-pressure valve 180A as an example. However, the liquid chromatography system 10 can also perform cleaning based on the first cleaning mode to the fifth cleaning mode for the flow paths including the high-pressure valves 180B to 180D, respectively. The above description is also applicable to the flow paths including the high-pressure valves 180B to 180D, respectively.

[0167] Next, refer to Figure 16 to Figure 19 An example will be described in which the flow channels configured in the liquid chromatography system 10 are cleaned in various cleaning modes when a sample is analyzed or prepared for analysis using one of the first to fourth analysis flow channels 291A to 291D.

[0168] <Example of cleaning during sample suction>

[0169] Fig.16 1 is a diagram showing an example of cleaning the flow path by the third cleaning mode and the fourth cleaning mode during the aspiration of the sample. In particular, an example is described here in which the first analysis flow path 291A is cleaned by the third cleaning mode and the second analysis flow path 291B is cleaned by the fourth cleaning mode during the aspiration of the sample.

[0170] Fig.16 In the embodiment, the first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180A. In the diverter valve 90, the port 91 toward the column 230A is connected to the port 95 toward the detector 500. Therefore, in the first analysis flow path 291A including the high pressure valve 180A, the sample can be analyzed.

[0171] The metering pump 130 is connected to the needle 191 via the low pressure valve 170, the second switching valve 160, the high pressure valve 180A, the first switching valve 150, and the needle valve 260. The needle 191 is guided to the container 302A containing the sample. The needle 191 sucks the sample from the container 302A by the negative pressure given by the metering pump 130.

[0172] In the high-pressure valve 180B, the port 183B and the port 184B are connected.

[0173] In this state, the liquid chromatography system 10 can execute cleaning in the third cleaning mode for the first analysis channel 291A including the high pressure valve 180A and cleaning in the fourth cleaning mode for the second analysis channel 291B including the high pressure valve 180B.

[0174] The rinse liquid supplied from the cleaning pump 143A to the high-pressure valve 180A flows through the high-pressure valve 180A, the column 230A, and the diverter valve 90 to clean these components and the flow path toward the detector 500 (third cleaning mode).

[0175] The flushing liquid supplied from the cleaning pump 143B to the high-pressure valve 180B flows through the high-pressure valve 180B, the column 230B, and the diverter valve 90 to clean the flow path including these components (fourth cleaning mode).

[0176] In this way, the liquid chromatography system 10 can clean the first analysis flow path 291A while continuing the operation of sucking the sample in order to analyze the sample in the first analysis flow path 291A. Furthermore, the liquid chromatography system 10 can clean the second analysis flow path 291B. In addition, the liquid chromatography system 10 can of course clean the third analysis flow path 291C including the high-pressure valve 180C and the fourth analysis flow path 291D including the high-pressure valve 180D at the same time.

[0177] <Example of cleaning during sample injection>

[0178] Fig.17 1 is a diagram showing an example of cleaning the flow path in the fourth cleaning mode during sample injection. In particular, an example is described here in which the second analysis flow path 291B is cleaned in the fourth cleaning mode when the sample is injected into the column 230A of the first analysis flow path 291A.

[0179] Fig.17 In the flow divider 90 , the first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180A. In the flow divider 90 , the port 91 facing the column 230A and the port 95 facing the detector 500 are connected.

[0180] The high-pressure pump 220A is connected to the needle 191 via the high-pressure valve 180A, the first switching valve 150, and the needle valve 260. The needle 191 is connected to the injection port 198A. The sample loop 192 holds the sample. The needle 191 injects the sample in the sample loop 192 into the injection port 198A together with the eluent supplied from the high-pressure pump 220A. Thus, the sample is injected into the column 230A via the high-pressure valve 180A.

[0181] In the high-pressure valve 180B, the port 183B and the port 184B are connected.

[0182] In this state, the liquid chromatography system 10 can perform cleaning based on the fourth cleaning mode with the second analysis flow path 291B including the high-pressure valve 180B as the object. That is, by supplying a flushing liquid from the cleaning pump 143B to the high-pressure valve 180B, the flow path including the high-pressure valve 180B, the column 230B and the diverter valve 90 can be cleaned (the fourth cleaning mode).

[0183] Thus, the liquid chromatography system 10 can clean the second analysis channel 291B while the sample is continuously injected into the column 230A in the first analysis channel 291A. In addition, the liquid chromatography system 10 can also clean the third analysis channel 291C including the high pressure valve 180C and the fourth analysis channel 291D including the high pressure valve 180D.

[0184] <First example of cleaning during sample analysis>

[0185] Fig.18 2 is a diagram showing an example of cleaning the flow path in the second cleaning mode during sample analysis. In particular, an example is described here in which the second analysis flow path 291B is cleaned in the second cleaning mode when the sample is analyzed using the first analysis flow path 291A.

[0186] Fig.18 In the embodiment, the high pressure pump 220A is connected to the column 230A via the high pressure valve 180A. The column 230A is loaded with a sample. In the diverter valve 90, the port 91 toward the column 230A is connected to the port 95 toward the detector 500. The eluent supplied from the high pressure pump 220A is injected into the column 230A loaded with the sample via the high pressure valve 180A. In the detector 500, the sample is analyzed.

[0187] The first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180B. The cleaning pump 143B is connected to the needle 191 via the high pressure valve 180B, the first switching valve 150, and the needle valve 260. The needle 191 is guided to the injection port 198B.

[0188] In this state, the liquid chromatography system 10 can perform cleaning in the second cleaning mode with the second analysis flow path 291B including the high pressure valve 180B as the target. That is, by supplying the cleaning liquid from the cleaning pump 143B to the high pressure valve 180B, the cleaning liquid flows in the order of the high pressure valve 180B, the first switching valve 150, the needle valve 260, the sample loop 192, the needle 191, the injection port 198B, the high pressure valve 180B, the column 230B and the diverter valve 90, and the flow path including the above parts is cleaned (second cleaning mode).

[0189] Thus, the liquid chromatography system 10 can clean the second analysis channel 291B in the second cleaning mode when analyzing the sample in the first analysis channel 291A. In addition, the liquid chromatography system 10 can of course clean the third analysis channel 291C including the high pressure valve 180C or the fourth analysis channel 291D including the high pressure valve 180D in the second cleaning mode instead of the second analysis channel 291B.

[0190] Furthermore, the liquid chromatography system 10 can also clean the second analysis channel 291B in the fourth mode when analyzing a sample in the first analysis channel 291A. Furthermore, the liquid chromatography system 10 can also clean the second analysis channel 291B in the second cleaning mode and clean the third analysis channel 291C in the fourth cleaning mode when analyzing a sample in the first analysis channel 291A.

[0191] <Second example of cleaning during sample analysis>

[0192] Fig.19 1 is a diagram showing an example of cleaning the flow path by the fourth cleaning mode and the fifth cleaning mode in the analysis of the sample. In particular, the following example is described here, that is, when the first analysis flow path 291A is used to analyze the sample, the second analysis flow path 291B is cleaned by the fourth cleaning mode, and the flow path including the high-pressure valve 180A is cleaned by the fifth cleaning mode.

[0193] Fig.19 In the detector 500, the first switching valve 150 and the second switching valve 160 are connected to the high pressure valve 180A. In the diverter valve 90, the port 91 toward the column 230A is connected to the port 95 toward the detector 500. The eluent supplied from the high pressure pump 220A is injected into the column 230A loaded with the sample through the high pressure valve 180A. In the detector 500, the sample is analyzed.

[0194] In the needle valve 260, the port 262 is connected to the port 263. In the high-pressure valve 180B, the port 183B is connected to the port 184B.

[0195] In this state, the liquid chromatography system 10 can execute cleaning in the fifth cleaning mode for the channel including the high-pressure valve 180A and cleaning in the fourth cleaning mode for the second analysis channel 291B including the high-pressure valve 180B.

[0196] The flushing liquid supplied from the needle cleaning pump 20 to the needle valve 260 flows through the needle valve 260, the sample loop 192, the needle 191, and the high-pressure valve 180A, and the flow path including these parts is cleaned (fifth cleaning mode).

[0197] The flushing liquid supplied from the cleaning pump 143B to the high-pressure valve 180B flows through the high-pressure valve 180B, the column 230B, and the diverter valve 90, and the flow path including these parts is cleaned (fourth cleaning mode).

[0198] In this way, while the liquid chromatography system 10 continues to analyze the sample using the first analysis channel 291A, it is possible to perform cleaning based on the fifth cleaning mode for the channel including the high-pressure valve 180A and cleaning based on the fourth cleaning mode for the second analysis channel 291B including the high-pressure valve 180B.

[0199] In addition, the liquid chromatography system 10 can of course clean both the third analysis channel 291C including the high-pressure valve 180C and the fourth analysis channel 291D including the high-pressure valve 180D in the fourth cleaning mode.

[0200] <Selectable cleaning modes>

[0201] Fig. 20 It is a diagram showing the cleaning modes selectable in the first to fourth analysis flow paths 291A to 291D. Fig. 20 In FIG. 1 , the types of cleaning modes selectable at each stage of three processes performed using the first analysis channel 291A are shown corresponding to each of the first to fourth analysis channels 291A to 291D.

[0202] If you have used Figure 10 to Figure 19 By organizing the various cleaning modes described above, for example, when the first analysis flow path 291A is used for sample analysis, the types of cleaning modes for cleaning the first analysis flow path 291A to the fourth analysis flow path 291D can be selected as follows: Fig. 20 shown.

[0203] The flow path cleaned by the fifth cleaning mode is a flow path where the cleaning liquid flows toward the drain pipe ports 181A to 184A of the high-pressure valves 180A to 180D. Fig. 20In FIG. 1 , the fifth cleaning mode is described in association with the first to fourth analysis channels 291A to 291D using the position notation of the cleaning modes associated with the first to fourth analysis channels 291A to 291D.

[0204] Fig. 20 The stages of sample aspiration, sample injection and eluent injection shown respectively refer to: the stage of aspirating the sample through the needle 191, the stage of injecting the aspirated sample from the needle 191 into the column 230A via the injection port 198A and the high-pressure valve 180A, and the stage of injecting the eluent supplied from the high-pressure pump 220A to the high-pressure valve 180A into the column 230A.

[0205] When the sample is sucked through the needle 191, the first to fourth analysis channels 291A to 291D can be cleaned in the third cleaning mode or the fourth cleaning mode. For example, the first analysis channel 291A can be cleaned in the third cleaning mode, and the second to fourth analysis channels 291B to 291D can be cleaned in the third cleaning mode.

[0206] When the sample sucked from the needle 191 is injected into the column 230A via the injection port 198A and the high pressure valve 180A, the second to fourth analysis channels 291B to 291D can be cleaned in the third cleaning mode or the fourth cleaning mode. For example, the second analysis channel 291B can be cleaned in the third cleaning mode, and the third and fourth analysis channels 291C and 291D can be cleaned in the fourth cleaning mode.

[0207] When the eluent supplied from the high pressure pump 220A to the high pressure valve 180A is injected into the column 230A, the first analysis flow path 291A can be cleaned in the fifth cleaning mode. The parts cleaned at this time are the needle valve, the sample loop 192, the needle 191, the injection port 198A, the port 182A of the high pressure valve 180A, and the port 181A of the high pressure valve 180A.

[0208] When the eluent supplied from the high pressure pump 220A to the high pressure valve 180A is injected into the column 230A, the second to fourth analysis flow paths 291B to 291D can be cleaned in any one of the second cleaning mode, the fourth cleaning mode, and the fifth cleaning mode. For example, the second analysis flow path 291B can be cleaned in the second cleaning mode, and the third analysis flow path 291C and the fourth analysis flow path 291D can be cleaned in the fourth cleaning mode.

[0209] In this way, the liquid chromatography system 10 can clean the first to fourth analytical channels 291A to 291D in various cleaning patterns. The liquid chromatography system 10 receives input of a cleaning pattern and a cleaning timing for cleaning each analytical channel.

[0210] The user uses the input device 120 (see Figure 4 ) to set the cleaning mode and cleaning timing for cleaning each analytical flow path. The set contents are displayed on the display device 125 (refer to Figure 4 ). Control device 110 (refer to Figure 4 ) According to user instructions input to the input device 120, the cleaning mode and cleaning timing for cleaning each analysis flow path are set.

[0211] <An example of cleaning mode setting>

[0212] Fig.21 This is a timing chart showing an example of the setting of the cleaning mode. Fig.21 In the example, (1) to (5) represent the first cleaning mode to the fifth cleaning mode, respectively. Fig.21 The flow of processing performed by the liquid chromatography system 10 according to the cleaning mode and cleaning timing set according to the user's instruction will be described.

[0213] The analysis using the sample is performed using the first analysis flow path 291A to the fourth analysis flow path 291D in sequence. First, the first analysis flow path 291A is used as the object for cleaning based on the first cleaning mode. As a result, the flow path including the high-pressure valve 180A, the first switching valve 150, the needle valve 260, the sample loop 192, the needle 191, the injection port 198A, the high-pressure valve 180A, the column 230A and the diverter valve 90 is cleaned by the flushing liquid. Furthermore, the flow path from the diverter valve 90 to the detector 500 is cleaned by the flushing liquid.

[0214] Next, the sample is sucked from the first analysis flow path 291A through the needle 191. While the sample is sucked from the needle 191, cleaning in the fourth cleaning mode is performed on the second to fourth analysis flow paths 291B to 291D. Thus, for example, in the second analysis flow path 291B, the flow path from the high pressure valve 180B to the column 230B and the flow path from the column 230B to the ports 96 and 97 of the diverter valve 90 are cleaned.

[0215] When the aspiration of the sample for the first analysis flow path 291A is completed, the sample is injected into the column 230A together with the eluent. When all the samples are injected from the needle 191, the cleaning based on the fifth cleaning mode is performed. Thus, the flow path including the needle valve 260, the sample loop 192, the needle 191, the injection port 198A and the high pressure valve 180A is cleaned.

[0216] When all the sample is injected from the needle 191 in the first analysis flow path 291A, the connection state of the high pressure valve 180A is switched, and a process of flowing the eluent to the sample injected into the column 230A is started.

[0217] While analysis is being performed in the first analysis channel 291A, cleaning in the second cleaning mode is performed in the order of the second to fourth analysis channels 291B to 291D. When analysis is completed in the first analysis channel 291A, cleaning in the second cleaning mode and cleaning in the third cleaning mode are performed on the first analysis channel 291A.

[0218] Next, the process for analyzing the sample using the second analysis channel 291B is started. That is, the connection destination of the first switching valve 150 and the second switching valve 160 is switched from the high pressure valve 180A to the high pressure valve 180B. Then, cleaning in the first cleaning mode is performed on the second analysis channel 291B.

[0219] The following, such as Fig.21 As shown, the process of analyzing the sample using the second to fourth analysis channels 291B to 291D and the process of cleaning the first to fourth analysis channels 291A to 291D are repeatedly performed according to the same flow.

[0220] <Cleaning using a combination of rinse solution and eluent>

[0221] Fig. 22 The timing chart shows an example of the driving pattern of the cleaning pumps 143A to 143D and the high-pressure pumps 220A to 220D. When the liquid chromatography system 10 cleans the flow path in the first to fourth cleaning modes, the flushing liquid and the eluent (blank liquid) can be used as the cleaning liquid.

[0222] For example, when cleaning the first analysis flow path 291A, the cleaning pump 143A is first driven. Thus, the first analysis flow path is cleaned with the flushing liquid. At a time point after a time T1 has passed since the driving of the cleaning pump 143A, the high-pressure pump 220A is driven instead of the cleaning pump 143A. Thus, the first analysis flow path is cleaned with the eluent. At a time point after a time T2 has passed since the driving of the high-pressure pump 220A, the driving of the high-pressure pump 220A is stopped.

[0223] According to this driving mode, after washing with the flushing liquid, the eluent is allowed to flow. Therefore, in the columns 230A to 230D, the mobile phase containing the eluent can be set to a balanced state. This driving mode can also be adopted in all the first washing mode to the fourth washing mode. For example, Fig.21In the cleaning mode settings shown, you can also Fig. 22 The wash solutions are combined with the eluents as shown.

[0224] Here, the driving of high-pressure pump 220A is stopped at the time point when time T2 has passed since the driving of high-pressure pump 220A. However, the driving of high-pressure pump 220A may be kept constant and may be driven at all times except when the rinse liquid is supplied from washing pump 143A.

[0225] <Processing flow (settings)>

[0226] Fig.23 FIG. 1 is a flowchart of a process for accepting input of analysis-related settings from a user in the liquid chromatography system 10. In one implementation example, Fig.23 The illustrated processing is realized by the processor 111 executing a given program.

[0227] In step S100, the liquid chromatography system 10 displays a setting screen on the display device 125. The setting screen accepts input of information (eg, compound name) for specifying an analysis target. Fig.24 This is an example of a setting screen. Fig.24 As shown, the setting screen 2400 includes an input field 2401 for accepting input of information specified as an analysis target.

[0228] return Fig.23 In step S102, the liquid chromatography system 10 obtains the information input on the setting screen to determine the analysis object and writes it to the memory 112. Then, the liquid chromatography system 10 ends. Fig.23 processing.

[0229] <Method File Database>

[0230] Fig.25 2 is a diagram schematically showing an example of the data structure of the method file database. The method file database includes two or more cleaning method files (hereinafter referred to as method files). Each method file defines the content of the analysis and cleaning performed by the liquid chromatography system 10.

[0231] More specifically, Fig.25 Four method files (1) to (4) are shown in FIG. Method files (1) to (4) respectively include an analysis method and a cleaning method. That is, in the method file database, one or more analysis methods (analysis conditions) are respectively combined with one or more cleaning methods.

[0232] Fig.25In the example of , the analysis method contained in the method file (1) includes a value R1 as a set value of the eluent flow rate. According to the method file (1), the liquid chromatography system 10 controls the high-pressure pump (high-pressure pump 220A, etc.) during the analysis to send the eluent to the column (column 230A, etc.) at a flow rate of R1.

[0233] The cleaning method included in the method file (1) has set values ​​for "execution conditions", "target sample" and "cleaning contents".

[0234] "Execution conditions" refers to the conditions for selecting each method file. In method file (1), the setting value of "Execution conditions" includes "QC value ≥ V1". QC value refers to the analysis result used for quality management of the flow path, which indicates the residual amount of the compound in the flow path. The larger the QC value, the greater the residual amount. For a specific example of the calculation method of QC value, please refer to Fig.26 The setting value of the execution condition "QC value ≥ V1" means that when the value of the analysis result is greater than V1, the method file (1) is selected.

[0235] The "target sample" refers to an analysis target in the liquid chromatography system 10. In one implementation example, the liquid chromatography system 10 determines the analysis target (target sample) based on the information input on the setting screen in step S102.

[0236] In the method file (1), the setting value of "target sample" includes "K1". The setting value of the target sample is "K1", which means that the method file (1) is selected when the target sample is "K1".

[0237] "Cleaning content" refers to the content implemented in the cleaning of the flow path. In the method file (1), the value of "Cleaning content" includes "First cleaning mode [10min]" and "Second cleaning mode [10min]". This means: in order to clean the flow path, the first cleaning mode ( Fig.11 ) for ten minutes, and then, a second cleaning mode ( Fig.12 ) for ten minutes of cleaning.

[0238] Method file (2) has different setting values ​​of execution conditions and cleaning contents from method file (1). In method file (2), the setting value of "execution conditions" includes "QC value < V1".

[0239] In method file (2), the value of "cleaning content" includes "first cleaning mode [5 min]" and "second cleaning mode [5 min]". This means that in order to clean the flow path, the first cleaning mode ( Fig.11 ) for five minutes, followed by a second cleaning mode ( Fig.12) for five minutes of cleaning.

[0240] Fig.25 In the example, when the target sample is "K1", if the QC value is ≥ V1, method file (1) is selected. On the other hand, when the target sample is "K1", if the QC value is < V1, method file (2) is selected. In method file (1), the time for implementing each first cleaning mode and second cleaning mode is longer than that in method file (2). The QC value indicates the amount of residual compound in the flow path. That is, in this embodiment, the more the residual compound in the flow path, the longer the cleaning method with the longer cleaning time is selected.

[0241] The method file (3) has different setting values ​​for the target sample and the cleaning content from the method file (1). In the method file (3), the setting value for "target sample" includes "other than K1".

[0242] In the method file (3), the value of "cleaning content" includes "first cleaning mode [9 min]" and "second cleaning mode [9 min]". This means that in order to clean the flow path, the first cleaning mode ( Fig.11 ) for nine minutes, followed by a second cleaning mode ( Fig.12 ) for nine minutes of cleaning.

[0243] Fig.25 In the example of , when the target sample is "K1", if the QC value is ≥ V1, method file (1) is selected. On the other hand, when the target sample is "other than K1", if the QC value is ≥ V1, method file (3) is selected.

[0244] The method file (4) has different setting values ​​for the target sample and the cleaning content from the method file (2). In the method file (4), the setting value for "target sample" includes "other than K1".

[0245] In the method file (4), the value of "cleaning content" includes "first cleaning mode [4min]" and "second cleaning mode [4min]". This means that in order to clean the flow path, the first cleaning mode ( Fig.11 ) for four minutes, followed by a second cleaning mode ( Fig.12 ) for four minutes of cleaning.

[0246] Fig.25 In the example of , when the target sample is "K1", if the QC value is <V1, method file (2) is selected. On the other hand, when the target sample is "other than K1", if the QC value is <V1, method file (4) is selected.

[0247] <Processing Flow (Analysis)>

[0248] Fig.26 is a flow chart of a process for analyzing a sample in a liquid chromatography system 10. In one implementation example, Fig.26 The illustrated processing is realized by the processor 111 executing a given program.

[0249] In step S200, the liquid chromatography system 10 is set to "1" as Fig.26 The value of variable N used in the processing. Variable N identifies the flow path used for analysis among the four flow paths. If the value of variable N is "1", the first flow path (first analysis flow path 291A) is used for analysis. If the value of variable N is "2", the second flow path (second analysis flow path 291B) is used for analysis. If the value of variable N is "3", the third flow path (third analysis flow path 291C) is used for analysis. If the value of variable N is "4", the fourth flow path (fourth analysis flow path 291D) is used for analysis.

[0250] In step S202, the liquid chromatography system 10 causes the high-pressure pump (any one of the high-pressure pumps 220A to 220D) in the flow path to send the eluent to the flow path used for analysis, thereby injecting the eluent toward the detector 500. The control of step S202 corresponds to so-called "blank injection".

[0251] In step S204, the liquid chromatography system 10 instructs the detector 500 to perform analysis. Accordingly, the detector 500 performs analysis of the eluate injected by the "blank injection".

[0252] In step S206 , the liquid chromatography system 10 obtains the analysis result of the eluate injected through the “blank injection” from the detector 500 .

[0253] In step S208, the liquid chromatography system 10 calculates the QC value based on the analysis result obtained in step S206. In one implementation example, the liquid chromatography system 10 obtains MS (mass spectrometry) data as the analysis result, and calculates the QC value as the height of the peak other than the peak caused by the eluent in the MS data.

[0254] In step S210 , the liquid chromatography system 10 reads the target sample acquired in step S102 from the memory 112 .

[0255] In step S212, the liquid chromatography system 10 determines the method file to be referred to in the current analysis from the plurality of method files included in the method file database based on the QC value calculated in step S208 and the target sample read in step S210. That is, the QC value calculated in step S208 satisfies the "execution condition" of the cleaning method of the determined method file. Furthermore, the target sample read in step S210 is included in the "target sample" of the cleaning method of the determined method file.

[0256] In step S214 , the liquid chromatography system 10 performs cleaning of the flow path using the cleaning method of the method file determined in step S212 .

[0257] In step S216 , the liquid chromatography system 10 displays the flow path information on the display device 125 . Fig. 27 This is a diagram showing an example of a screen displaying flow path information.

[0258] Fig. 27 The screen 2600 includes a graph 2601. The graph 2601 shows the time changes of the high-pressure pump 220A of the first flow path and the high-pressure pump 220B of the second flow path. The vertical axis on the left side of the graph 2601 shows the pressure value. The horizontal axis of the graph 2601 shows time.

[0259] Fig. 27 In the example of FIG. 1 , the pressure values ​​of the high-pressure pumps of the first flow path and the second flow path are shown. The pressure value of the high-pressure pump refers to the pressure of the liquid ejected by the high-pressure pump.

[0260] As long as the flow path information includes the pressure value of the high-pressure pump of the flow path used for analysis, it may include the pressure value of the high-pressure pump of all flow paths or only the pressure value of the high-pressure pump of a part of the flow paths. As the flow path information, the QC value calculated for each flow path may be further displayed. In one implementation example, the liquid chromatography system 10 continuously detects the pressure value of the high-pressure pump and continues to display the flow path information until the analysis method described later as step S218 is completed.

[0261] Refer again Fig.26 In step S218, the liquid chromatography system 10 performs analysis of the sample using the analysis method of the method file determined in step S212.

[0262] In step S220, the liquid chromatography system 10 updates the value of the variable N. More specifically, if the value of the variable N is "1" to "3", the liquid chromatography system 10 adds 1 to the value of the variable N and updates it in step S220. If the value of the variable N is "4", the value of the variable N is updated to "1". Thus, the value of the variable N cycles between "1" and "4". Subsequently, the liquid chromatography system 10 returns control to step S202.

[0263] According to the processing described above, the liquid chromatography system 10 obtains the analysis results for quality management of the flow paths at the beginning of the analysis using each flow path. In addition, the liquid chromatography system 10 determines the method file used for the analysis based on the analysis results for quality management. By determining the method file, the cleaning method used for the analysis is determined. The cleaning method specifies the method for controlling one or more pumps during cleaning by specifying the cleaning mode. The one or more pumps include at least one of the high-pressure pumps 220A to 220D and the cleaning pumps 143A to 143D. By determining the cleaning method for each flow path in this way, each flow path is cleaned according to the state of each flow path. Therefore, according to the present disclosure, a technology for properly cleaning each flow path is provided.

[0264] In the liquid chromatography system 10, a plurality of types of cleaning liquids may be connected to a cleaning pump. In the method file database, the cleaning method may also specify the type of cleaning liquid used for cleaning. The liquid chromatography system 10 determines a cleaning method by determining a cleaning method file in step S212. By determining a cleaning method, the type of cleaning liquid used for cleaning is determined. In step S214, the liquid chromatography system 10 may also control the connection form in the liquid chromatography system 10 so that only the cleaning liquid of the type determined to be used for cleaning among the plurality of cleaning liquids is connected to the cleaning pump.

[0265] The QC value obtained as the analysis result for quality management is not limited to the value based on MS data. The analysis result can also be calculated by using the analysis result based on the analysis method other than mass analysis. For example, the liquid chromatogram of the sample after the "blank injection" can also be used. In this case, the QC value can also be calculated based on the peak value of the peak caused by the compound other than the eluent according to the liquid chromatogram.

[0266] The cleaning mode includes the selection of which of the first to fifth cleaning modes and / or the length of time for executing the cleaning mode. Each of the high-pressure pumps 220A to 220D is an example of a driving pump that supplies a mobile phase to a flow channel.

[0267] In the liquid chromatography system 10, four flow paths (a first analysis flow path 291A, a second analysis flow path 291B, a third analysis flow path 291C, and a fourth analysis flow path 291D) are arranged in parallel. When the liquid chromatography system 10 performs cleaning according to the cleaning method on a certain flow path in step S214, cleaning according to the same cleaning method can also be performed on other flow paths. Thus, control in the liquid chromatography system 10 including a plurality of flow paths can be facilitated.

[0268] Furthermore, the liquid chromatography system 10 may also execute the cleaning method contained in the same cleaning method file as the cleaning method file containing the analysis method in the method file database after the analysis method is implemented in step S218. That is, the liquid chromatography system 10 may also drive the cleaning pump by the cleaning method combined with the analysis method in the method file database after the analysis according to certain analysis conditions.

[0269] In addition, when cleaning the other flow paths according to the cleaning method in step S214 , if the sample is being injected into the other flow paths, the liquid chromatography system 10 may clean the other flow paths after the sample injection is completed.

[0270] Furthermore, in the liquid chromatography system 10, the method file may be determined by using only the target sample. Fig.26 In the example of FIG. 2 , in step S212, the method file to be referred to in the current analysis is determined based on both the QC value calculated in step S208 and the target sample read in step S210. However, the method file to be referred to in the current analysis may be determined based only on the QC value calculated in step S208 or based only on the target sample read in step S210.

[0271] Fig.28 It is a diagram showing a first modified example of the method file database. Fig.28 In the method file database shown, no target sample is associated with the cleaning method. In this example, in step S212, the liquid chromatography system 10 determines the method file including the QC value calculated in step S208 as an execution condition as the method file to be referred to in the analysis.

[0272] Fig.29 It is a diagram showing a second modified example of the method file database. Fig.29 The method file database shown includes the target sample as the execution condition of the cleaning method. In this example, the liquid chromatography system 10, in step S212, determines the method file including the target sample read in step S210 as the execution condition as the method file referenced in the analysis. In this example, the "target sample" is an example of information that specifies the analysis target sample in the flow path used for analysis.

[0273] [form]

[0274] Those skilled in the art will appreciate that the multiple exemplary embodiments described are specific examples of the following forms.

[0275] (Item 1) A liquid chromatography system of one form may also include: a first column for separating a sample into each component; a first flow path, which is a flow path for analysis including the first column; one or more cleaning pumps for supplying cleaning liquid to the first flow path; a memory for storing two or more combinations of cleaning methods and cleaning execution conditions; and a processor, the processor being configured to use the first flow path to analyze the sample, obtain at least one of an analysis result for quality management of the first flow path and information for determining the analysis object sample in the first flow path, determine a first combination of execution conditions corresponding to the analysis result for quality management and at least one of the information from the two or more combinations, and drive the one or more cleaning pumps according to the method contained in the first combination.

[0276] According to the liquid chromatography system described in the first item, the flow path can be properly cleaned.

[0277] (Item 2) In the liquid chromatography system described in Item 1, the processor may acquire the analysis result for quality management, and the analysis result for quality management may include an analysis result of a residual amount of the component that has passed through the first flow path.

[0278] According to the liquid chromatography system described in the second aspect, the contaminants accumulated in the first flow channel are reflected in the analysis results for quality control together with the eluent used as the mobile phase.

[0279] (Item 3) In the liquid chromatography system described in Item 2, the analysis result for quality control may include an analysis result obtained by a mass spectrometer.

[0280] According to the liquid chromatography system described in the third aspect, the result of mass analysis of the foulants accumulated in the first flow path can be obtained.

[0281] (Item 4) In the liquid chromatography system described in any one of Items 1 to 3, the one or more cleaning pumps may include a driving pump for supplying the mobile phase to the first flow path, and the processor may display the discharge pressure of the driving pump and the time change of the analysis result for quality management.

[0282] According to the liquid chromatography system described in the fourth aspect, the user can view the analysis results for driving the pump and quality control.

[0283] (Item 5) The liquid chromatography system according to any one of Items 1 to 4 may further include: an input device for receiving input of the information.

[0284] According to the liquid chromatography system described in the fifth aspect, the user only needs to input the type of the next analysis target sample, and the flow path cleaning method can be set according to the type of the sample.

[0285] (Item 6) The liquid chromatography system described in any one of Items 1 to 5 may also include: a second flow path, arranged in parallel with the first flow path, and the processor executes control for cleaning the second flow path based on the first combination in response to the situation where the first combination has been determined for the first flow path.

[0286] According to the liquid chromatography system described in the sixth aspect, control in the liquid chromatography system including a plurality of flow paths can be facilitated.

[0287] (Item 7) In the liquid chromatography system described in Item 6, the processor may clean the second flow path in the same method as the first combination in response to the first combination being determined for the first flow path.

[0288] According to the liquid chromatography system described in the seventh item, a plurality of flow paths can be cleaned using the same method.

[0289] (Item 8) In the liquid chromatography system described in Item 6, when a sample is injected into the second flow path, the processor may execute control for cleaning the second flow path according to the first combination after the injection.

[0290] According to the liquid chromatography system described in the eighth aspect, it is possible to prevent the injection of the sample being performed in the second flow path from being wasted due to cleaning.

[0291] (Item 9) In the liquid chromatography system described in any one of Items 1 to 8, in the above two or more combinations, the execution condition may be combined with the method having a longer cleaning time as the amount of residual compounds in the first flow path indicated by the corresponding analysis results for quality management increases.

[0292] According to the liquid chromatography system described in item 9, even if a large amount of the compound remains in the first flow channel, the compound can be reliably removed by washing.

[0293] (Item 10) Another form of a liquid chromatography system may also include: a first flow path, including a flow path for analysis; one or more cleaning pumps, supplying cleaning liquid to the first flow path; a processor; and a memory, storing one or more analysis conditions and one or more cleaning methods, wherein the one or more analysis conditions are respectively combined with any one of the one or more cleaning methods in the memory, and the processor determines to use the first flow path for analysis of the sample, and after analysis based on any one of the one or more analysis conditions, drives the one or more cleaning pumps based on a cleaning method of the one or more cleaning methods combined with the one analysis condition.

[0294] According to the liquid chromatography system described in the tenth item, the flow path can be properly cleaned.

[0295] (Item 11) In the liquid chromatography system described in any one of Items 1 to 10, at least one of the one or more cleaning pumps may be connected to a plurality of cleaning liquids, and the processor may select a cleaning liquid from the plurality of cleaning liquids according to the cleaning method, and connect the selected cleaning liquid to the one or more cleaning pumps.

[0296] According to the liquid chromatography system described in item 11, the flow path can be cleaned using a cleaning solution that is appropriate for the cleaning method to be performed.

[0297] (Item 12) A cleaning method in one form may also be a cleaning method for a liquid chromatography system, wherein the liquid chromatography system comprises: a first flow path, comprising an analysis flow path having a first analysis column; one or more pumps for supplying liquid to the first flow path; and a memory for storing two or more combinations of cleaning methods and cleaning execution conditions, the cleaning method comprising the following steps: determining to use the first flow path for sample analysis; in response to a decision to use the first flow path for sample analysis, obtaining at least one of an analysis result for quality management of the first flow path and information for determining the analysis object sample in the first flow path; determining, from the two or more combinations, a first combination of execution conditions corresponding to the analysis result for quality management and at least one of the information; and driving the one or more pumps according to the method contained in the first combination.

[0298] According to the cleaning method described in the twelfth item, the flow path can be properly cleaned in the liquid chromatography system.

[0299] (Item 13) A computer-readable storage medium in one form may also be a non-temporary computer-readable storage medium having a program recorded thereon, wherein the program is executed by a processor of a controller to cause the controller to implement the following steps: in a liquid chromatography system, determining to use a first flow path including an analysis flow path having a first analysis column for sample analysis; in response to the determination to use the first flow path for sample analysis, obtaining at least one of an analysis result for quality management of the first flow path and information identifying the sample to be analyzed in the first flow path; determining a first combination of execution conditions corresponding to the analysis result for quality management and at least one of the information from two or more combinations of cleaning methods and cleaning execution conditions; and driving one or more pumps for supplying liquid to the first flow path in accordance with a method included in the first combination.

[0300] According to the computer-readable storage medium described in the thirteenth item, in the liquid chromatography system, the flow path can be properly cleaned.

[0301] The embodiments disclosed this time should be considered as illustrative in all aspects and not restrictive. The scope of the present invention is indicated by the claims rather than the description of the embodiments described above, and is intended to include all modifications within the meaning and scope equivalent to the claims.

Claims

1. A liquid chromatography system, characterized in that: include: The first column separates the sample into each component; The first flow path is a flow path for analysis including the first column; one or more cleaning pumps for supplying cleaning fluid to the first flow path; A memory storing two or more cleaning methods, wherein cleaning contents are associated with at least one of an analysis result for quality management and information identifying a sample to be analyzed; as well as processor, The processor is configured as follows: When analyzing a sample using the first flow channel, obtaining at least one of an analysis result for quality control of the first flow channel and information identifying the sample to be analyzed in the first flow channel, determining the cleaning content corresponding to at least one of the acquired analysis result for quality management of the first flow path and information identifying a sample to be analyzed in the first flow path, with reference to the two or more cleaning methods stored in the memory; The one or more cleaning pumps are driven according to the determined cleaning content.

2. The liquid chromatography system according to claim 1, characterized in that: The processor obtains the analysis result for quality management, The analysis result for quality control includes an analysis result of a residual amount of a component that has passed through the first flow path.

3. The liquid chromatography system according to claim 2, characterized in that: The analysis results for quality control include analysis results obtained by a mass analyzer.

4. The liquid chromatography system according to any one of claims 1 to 3, characterized in that The one or more cleaning pumps include a driving pump for supplying a mobile phase to the first flow path. The processor displays the time changes of the discharge pressure of the driving pump and the analysis result for quality management.

5. The liquid chromatography system according to any one of claims 1 to 3, characterized in that: Also includes: The input device receives input of the information.

6. The liquid chromatography system according to any one of claims 1 to 3, characterized in that: Also includes: A second flow path is provided in parallel with the first flow path, The processor executes control for cleaning the second flow path in accordance with the cleaning content in response to the fact that the cleaning content has been determined for the first flow path.

7. The liquid chromatography system according to claim 6, characterized in that: In response to the fact that the cleaning content has been determined for the first flow path, the processor cleans the second flow path using the same method as the cleaning content.

8. The liquid chromatography system according to claim 6, characterized in that: When a sample is injected into the second flow channel, the processor executes control for cleaning the second flow channel according to the cleaning content after the injection.

9. The liquid chromatography system according to any one of claims 1 to 3, characterized in that: Among the two or more cleaning methods, a cleaning method having a longer cleaning time is selected as the residual amount of the compound in the first flow channel indicated by the corresponding analysis result for quality control is larger.

10. The liquid chromatography system according to any one of claims 1 to 3, characterized in that: At least one of the more than one cleaning pumps is connected to a plurality of cleaning fluids, The processor selects a cleaning liquid from the plurality of cleaning liquids according to the cleaning method, and connects the selected cleaning liquid to the one or more cleaning pumps.

11. A cleaning method is a cleaning method for a liquid chromatography system, characterized in that: The liquid chromatography system comprises: a first flow path, comprising an analytical flow path having a first analytical column; one or more pumps, supplying liquid to the first flow path; and a memory storing two or more cleaning methods, wherein the cleaning contents are associated with at least one of the analysis results for quality management and the information identifying the sample to be analyzed, The cleaning method comprises the following steps: determining to use the first flow path for sample analysis; In response to a decision to use the first flow channel for sample analysis, obtaining at least one of an analysis result for quality management of the first flow channel and information identifying an analysis target sample in the first flow channel; determining the cleaning content corresponding to at least one of the acquired analysis result for quality management of the first flow path and information identifying a sample to be analyzed in the first flow path, with reference to the two or more cleaning methods stored in the memory; and The one or more pumps are driven according to the determined cleaning content.

12. A computer-readable storage medium is a non-transitory computer-readable storage medium having a program recorded thereon, characterized in that: The program is executed by a processor of the controller to cause the controller to implement the following steps: In a liquid chromatography system, a memory is used to store two or more cleaning methods, wherein cleaning contents are associated with at least one of an analysis result for quality control and information identifying a sample to be analyzed, and a first flow path including an analysis flow path having a first analysis column is determined to be used for analysis of the sample; In response to a decision to use the first flow channel for sample analysis, obtaining at least one of an analysis result for quality management of the first flow channel and information identifying an analysis target sample in the first flow channel; determining the cleaning content corresponding to at least one of the acquired analysis result for quality management of the first flow path and information identifying a sample to be analyzed in the first flow path, with reference to the two or more cleaning methods stored in the memory; and One or more pumps that supply liquid to the first flow path are driven according to the determined cleaning content.

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