Microfluidic detection chip

Abstract

The present invention provides a microfluidic detection chip, which includes a sheet-like body and a plurality of detection units arranged on the body, wherein the plurality of detection units are radially arranged on the body, and the detection units include a sample area, a reagent area, a detection area, and a waste liquid storage area. The sample area is at least used for injecting samples; the reagent area is used for injecting functional reagents, and the reagent area includes a reagent tank, which is connected to the sample area through a first microchannel; the detection area is used for performing a mixing reaction of the functional reagent and the sample, and the detection area and the reagent area are connected through a second microchannel; the waste liquid storage area is used to hold the functional reagent and sample after the reaction, and the waste liquid storage area is connected to the detection area through a third microchannel. The microfluidic detection chip of the present invention can realize multi-person multi-project detection, has a simple structure, and can reduce the production and manufacturing costs and detection costs of the microfluidic detection chip.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro microfluidic detection, and in particular to a microfluidic detection chip. Background Art

[0002] Microfluidic detection chip technology integrates the basic operational units of biological, chemical, and medical analysis processes, such as sample preparation, reaction, separation, and detection, onto a micron-scale chip, automating the entire analysis process. It holds enormous potential in fields such as biology, chemistry, and medicine.

[0003] However, the existing microfluidic detection chip technology still has some defects in practical applications, such as complex structure, large sample usage, inaccurate detection results, single detection items and test samples, and high production costs. Summary of the Invention

[0004] The main purpose of the present invention is to provide a microfluidic detection chip to solve the problem of single detection items and single detection samples in existing microfluidic detection chips.

[0005] To achieve the above-mentioned object, the present invention provides a microfluidic detection chip, comprising a sheet-like body and a plurality of detection units arranged on the body, wherein the plurality of detection units are radially arranged on the body, and the detection units include:

[0006] a sample area, the sample area being at least used for sample injection;

[0007] A reagent area, the reagent area is used for injecting functional reagents, the reagent area includes a reagent tank, and the reagent tank is connected to the sample area through a first microfluidic channel;

[0008] a detection zone, the detection zone being used for performing a mixing reaction of a functional reagent and a sample, the detection zone being connected to the reagent zone via a second microfluidic channel; and

[0009] The waste liquid storage area is used to hold functional reagents and samples after the reaction, and the waste liquid storage area is connected to the detection area through a third microchannel.

[0010] Furthermore, the sample area includes a sample separation unit, and the sample separation unit area includes:

[0011] An injection slot, used for sample injection;

[0012] a plasma / serum tank, the plasma / serum tank being connected to the injection tank and being used to temporarily store the separated plasma / serum;

[0013] a blood cell tank, the blood cell tank being located on a side of the plasma / serum tank away from the injection tank and being connected to the plasma / serum tank, and being used for temporarily storing the separated blood cells; and

[0014] A storage tank is connected to the plasma / serum tank and is used to quantify the sample injection amount.

[0015] Furthermore, a connection area is provided on a side of the plasma / serum tank away from the injection tank, and the first microchannel is connected to the plasma / serum tank through the connection area;

[0016] The sample separation unit further includes a first connecting channel, the blood cell tank is connected to the connecting area via the first connecting channel, and the depths of the first connecting channel and the first microfluidic channel are both smaller than the depth of the connecting area.

[0017] Furthermore, a first microfluidic valve is provided on the first microfluidic channel, and a depth of the first microfluidic valve is at least 1 mm deeper than a depth of the first microfluidic channel; and / or,

[0018] A second microfluidic valve is provided on the third microfluidic channel, and a depth of the second microfluidic valve is at least 1 mm deeper than a depth of the second microfluidic channel.

[0019] Furthermore, the detection unit further includes a buffer zone, the third microchannel is connected between the detection zone and the buffer zone, and the buffer zone is connected to the waste liquid storage zone through a second connecting channel.

[0020] Furthermore, the buffer area is connected to the waste liquid storage area through at least two of the second connecting channels.

[0021] Furthermore, the volume of the waste liquid storage area is greater than the volume of the buffer area, wherein:

[0022] The waste liquid storage area is located on a side of the detection area away from the reagent area, and the waste liquid storage area includes a first edge arranged close to the buffer zone, the first edge includes a first end area, a middle area and a second end area, the first end area, the middle area and the second end area are arranged in sequence along the circumference of the body, and the first end area is connected to the middle area by a first recessed area, and the middle area is connected to the second end area by a second recessed area, the first recessed area and the second recessed area are recessed in a direction away from the buffer zone, and the first end area and the second end area are protruding in a direction close to the buffer zone, and the connection position of the second connecting channel and the waste liquid storage area is located in the middle area.

[0023] Furthermore, the detection unit further includes a stop groove, and the stop groove is arranged on a side of the waste liquid storage area away from the detection area.

[0024] Furthermore, the stop groove extends along the circumference of the body, and the length of the stop groove is not less than the length of the waste liquid storage area along the circumference of the body.

[0025] Furthermore, the third microchannel has a protrusion protruding toward the center of the body, and the distance from the vertex of the protrusion to the center of the body is not less than the distance from one end of the detection area close to the center of the body to the center of the body.

[0026] The present invention utilizes a microfluidic detection chip with multiple detection units, each with a simple structure. Simply controlling the rotational speed and direction of the detection instrument allows for the sequential release of functional reagents and samples. Furthermore, the microfluidic detection chip can perform multi-person, multi-item testing, and its simple structure reduces both manufacturing and testing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are intended to explain the present invention and do not constitute an undue limitation of the present invention. In the accompanying drawings:

[0028] Figure 1 is a top view of the microfluidic detection chip disclosed in an embodiment of the present invention;

[0029] Figure 2 is a top view of a detection unit of a microfluidic detection chip disclosed in an embodiment of the present invention;

[0030] Figure 3 This is a sample separation flow chart of the microfluidic detection chip disclosed in an embodiment of the present invention;

[0031] Figure 4 This is a cleaning flow chart of the microfluidic detection chip disclosed in an embodiment of the present invention;

[0032] Figure 5 This is a flow chart of the flow channel emptying of the microfluidic detection chip disclosed in an embodiment of the present invention.

[0033] The above drawings include the following reference numerals:

[0034] 10. Main body; 20. Detection unit; 21. Sample area; 211. Injection slot; 212. Plasma / serum slot; 213. Blood cell slot; 214. Storage slot; 215. Connection area; 216. First connection channel; 217. First vent hole; 218. First vent slot; 22. Reagent area; 221. Reagent slot; 222. Reagent injection position; 23. Detection area; 24. Waste liquid storage area; 241. First edge; 2411. First An end region; 2412, a middle region; 2413, a second end region; 2414, a first recessed region; 2415, a second recessed region; 242, a second edge; 25, a first microfluidic channel; 26, a second microfluidic channel; 27, a third microfluidic channel; 271, a raised portion; 28, a second microfluidic valve; 29, a buffer zone; 201, a second connecting channel; 202, a stop groove; 203, a second air hole; 204, a second air groove. DETAILED DESCRIPTION

[0035] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0036] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0037] Unless otherwise specifically stated, the relative arrangement of the parts and steps, the numerical expressions and the numerical values set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific values should be interpreted as being merely exemplary and not as limiting. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0038] See also Figure 1 and Figure 2 As shown, Figure 1 is a top view of the microfluidic detection chip disclosed in an embodiment of the present invention, Figure 2 It is a top view of the detection unit of the microfluidic detection chip of the present invention. According to an embodiment of the present invention, a microfluidic detection chip is provided, which includes an upper layer of a chip (not shown in the figure), an intermediate layer (not shown in the figure) and a main body 10. Among them, the upper layer of the chip serves as the shell part of the microfluidic detection chip and protects the main body 10. The intermediate layer covers the main body 10 and can seal the main body 10. The upper layer and the intermediate layer of the chip are provided with injection holes for samples or reagents to pass through. During actual assembly, the upper layer of the chip, the intermediate layer and the main body 10 can be fixed together by bonding, clamping, screwing or welding.

[0039] Specifically, the body 10 of the microfluidic detection chip in this embodiment is arranged in a thin sheet shape. The thin sheet-shaped body 10 can be a circular sheet structure, a polygonal sheet structure or other special-shaped sheet structures. The drawings in this embodiment show the situation when the body 10 is arranged in a circular sheet structure. The surface of the body 10 is provided with a plurality of detection units 20. The plurality of detection units 20 are radially arranged on the body 10. The plurality of detection units 20 here can be two, three, four, five or more than five. The appendix in this embodiment Figure 1 , which shows the case where there are 12 detection units 20. It is understood that the shape of each detection unit 20 in this embodiment is generally fan-shaped (the outer contour of the detection unit 20 is generally fan-shaped). In this case, the multiple detection units 20 are radially arranged on the body 10, which means that the center side of each detection unit 20 is close to the center position of the body 10, the radial direction is consistent with the radial direction of the body 10, and the multiple detection units 20 are arranged in sequence along the circumference of the body 10.

[0040] Furthermore, the detection unit 20 includes a sample area 21, a reagent area 22, a detection area 23, and a waste liquid storage area 24. The sample area 21 is at least used for injecting a sample, and the sample mentioned here can be, for example, a blood sample. Of course, in other embodiments of the present invention, the sample can also be urine, etc. The reagent area 22 is used for injecting a functional reagent, and the reagent area 22 includes a reagent tank 221, which is connected to the sample area 21 through a first microchannel 25. The detection area 23 is used for performing a mixing reaction of the functional reagent and the sample, and the detection area 23 and the reagent area 22 are connected through a second microchannel 26. The waste liquid storage area 24 is used to hold the functional reagent and sample after the reaction, and the waste liquid storage area 24 is connected to the detection area 23 through a third microchannel 27.

[0041] In actual use, the microfluidic detection chip of this embodiment is placed in a matching detection instrument. At this time, the sampling needle and reagent needle on the detection instrument can be used to add samples and functional reagents to the sample area 21 and reagent area 22, respectively. After the sample enters the sample area 21, the detection instrument can drive the microfluidic detection chip to rotate, transporting the sample from the first microchannel 25 to the reagent area 22, and then through the second microchannel 26 to the detection area 23. Similarly, the functional reagent that enters the reagent area 22 is affected by centrifugal force during the rotation of the microfluidic detection chip and can be transported to the detection area 23 through the second microchannel 26. During this process, the testing personnel can control the rotation speed of the testing instrument according to the actual testing needs and testing items to realize the sequential release of functional reagents and samples. When the functional reagents and samples enter the detection area 23, a mixing reaction can be carried out in this area. Thereafter, the detection area 23 can be incubated, cleaned, and other operations can be performed. The cleaning liquid can be transported to the waste liquid storage area 24 through the third microchannel 27 under the action of centrifugal force. Finally, the detection area 23 is optically detected by the optical path detection device on the detection instrument, and the signal obtained by the detection is transmitted to the control unit on the detection instrument. The corresponding detection result can be obtained by calculation by the control unit.

[0042] Compared to existing microfluidic detection chips, the microfluidic detection chip in this embodiment is equipped with multiple detection units 20. Each detection unit 20 has a simple structure, and the functional reagents and samples can be released sequentially by simply controlling the rotation speed and direction of the detection instrument. Furthermore, this microfluidic detection chip can perform multi-person, multi-project testing, and its simple structure can reduce the production and testing costs of the microfluidic detection chip.

[0043] The various areas of the microfluidic detection chip will be described in detail below with reference to the accompanying drawings.

[0044] See also Figure 2 As shown, the sample area 21 in this embodiment includes a sample separation unit, which can be used to separate and inject samples. The sample separation unit here is particularly suitable for separating blood samples. Specifically, the sample separation unit includes an injection groove 211, a plasma / serum groove 212, a blood cell groove 213 and a storage groove 214. Among them, the injection groove 211 is used for sample injection; the plasma / serum groove 212 is connected to the injection groove 211, and the plasma / serum groove 212 is used to temporarily store the separated plasma / serum; the blood cell groove 213 is located on the side of the plasma / serum groove 212 away from the injection groove 211 and is connected to the plasma / serum groove 212, and the blood cell groove 213 is used to temporarily store the separated blood cells; the storage groove 214 is connected to the plasma / serum groove 212, and the storage groove 214 is used to quantify the sample injection amount.

[0045] See also Figure 3 As shown, Figure 3 The sample separation flow chart of the microfluidic detection chip in this embodiment is shown. Specifically, a whole blood sample is injected into injection slot 211 via a sample needle, etc. Afterwards, the detection instrument drives the microfluidic detection chip to rotate in a first direction to achieve centrifugal separation of the sample. Blood cells have a greater mass than plasma / serum. After centrifugation, blood cells enter blood cell slot 213, which is farther from injection slot 211. After centrifugation, plasma / serum enters plasma / serum slot 212, which is closer to injection slot 211. Excess whole blood enters storage slot 214. The sample separation unit of the present invention can process whole blood samples with a high hematocrit ratio (plasma / serum: 30%, blood cells: 70%).

[0046] Optionally, in this embodiment, a first air hole 217 is further provided on the sample area 21. The first air hole 217 is connected to the storage tank 214 through a first air groove 218. The sample area 21 can be emptied through the first air hole 217 and the first air groove 218.

[0047] In the specific design, the injection groove 211, the plasma / serum groove 212 and the blood cell groove 213 are arranged in a straight line and are arranged in sequence from the inner side of the body 10 (the side close to the center of the body 10) to the outer side (the side close to the outer edge of the body 10). The storage groove 214 is located on the first side of the straight line formed by the injection groove 211, the plasma / serum groove 212 and the blood cell groove 213 ( Figure 2 The first microchannel 25 is provided on the second side of the straight line formed by the injection tank 211, the plasma / serum tank 212 and the blood cell tank 213 ( Figure 2 Correspondingly, the reagent area 22 is located on the second side of the straight line formed by the injection groove 211, the plasma / serum groove 212 and the blood cell groove 213 to be connected with the first microchannel 25. Such an arrangement can make the layout of the entire detection unit 20 more compact. When the surface size of the main body 10 is the same, more detection units 20 can be set, which can further improve the detection throughput of the microfluidic detection chip in this embodiment.

[0048] Furthermore, a connection area 215 is provided on the side of the plasma / serum groove 212 away from the injection groove 211. The connection area 215 is a recessed area extending from the side of the plasma / serum groove 212 away from the injection groove 211 and having a width smaller than that of the plasma / serum groove 212. The recessed area is located on the side of the plasma / serum groove 212 close to the detection area 23. The first microchannel 25 is connected to the plasma / serum groove 212 through the connection area 215. With such a configuration, when the microfluidic detection chip rotates along the second direction (the direction opposite to the first direction), the serum or plasma in the plasma / serum groove 212 is gathered in the connection area 215, which is more suitable for fully, comprehensively and quickly transporting the plasma or serum in the plasma / serum groove 212 to the reagent area 22, thereby reducing the amount of sample used to a certain extent.

[0049] Optionally, the sample separation unit also includes a first connecting channel 216, and the blood cell groove 213 is connected to the connecting area 215 through the first connecting channel 216. The depth of the first connecting channel 216 (the depth along the thickness direction of the main body 10) and the depth of the first microchannel 25 (the depth along the thickness direction of the main body 10) are less than the depth of the connecting area 215 (the depth along the thickness direction of the main body 10). Specifically, the depth of the connecting area 215 is at least 0.5 mm deeper than the depth of the first microchannel 25 and the first connecting channel 216, for example, 0.5 mm, 1 mm, etc. With such an arrangement, a partition area can be formed between the first microchannel 25 and the connecting area 215, and between the first connecting channel 216 and the connecting area 215, thereby avoiding multiple releases of the sample and improving the detection accuracy of the microfluidic detection chip in this embodiment.

[0050] In some embodiments of the present invention, a first microfluidic valve (not shown in the figure) is provided on the first microfluidic channel 25. The depth of the first microfluidic valve is deeper than that of the first microfluidic channel 25. Specifically, the depth of the first microfluidic valve is at least 1 mm deeper than that of the first microfluidic channel 25, for example, 1 mm, 1.2 mm, 1.4 mm, 1.6 mm, etc. By providing the first microfluidic valve on the first microfluidic channel 25, it is possible to further prevent the sample in the plasma / serum tank 212 from being released into the reagent area 22 multiple times, and it is also possible to prevent the liquid in the reagent area 22 from flowing back into the sample area 21, thereby further improving the detection accuracy of the microfluidic detection chip.

[0051] For example, in this embodiment, the first microchannel 25 is arranged in a U-shape or a V-shape, and the connection area 215 is closer to the center of the main body 10 than the main area of the reagent area 22 (i.e., the reagent tank 221). Such an arrangement is more suitable for establishing a siphon structure to transport the sample in the plasma / serum tank 212 to the reagent area 22 through the first microchannel 25.

[0052] Recombination Figure 1 and Figure 2As shown, the reagent area 22 in this embodiment includes a reagent tank 221 and at least two reagent injection positions 222, and the reagent injection positions 222 are connected to the reagent tank 221. Specifically, the reagent injection positions 222 in this embodiment can be set to two, three, four or more than four, and are designed and processed according to actual detection requirements. By setting at least two reagent injection positions 222 in the reagent area 22, different reagents can be injected from different reagent injection positions 222 during the actual use of the microfluidic detection chip, which can reduce the contamination rate during the reagent addition process and improve the detection accuracy of the microfluidic detection chip.

[0053] The accompanying drawings in this embodiment show the situation when there are two reagent injection positions 222. One of the two reagent injection positions 222 is located on the side of the injection groove 211 close to the center of the main body 10, and is connected to the reagent groove 221 through a fine channel. The other reagent injection position 222 is located on the inner side of the first microchannel 25, that is, the first microchannel 25 with a U-shaped or V-shaped arrangement is surrounded to form an inner area. In this way, the setting position of the reagent injection position 222 just fills the blank area of the detection unit 20. The structure of the entire detection unit 20 is compact, which is more suitable for setting multiple detection units 20 on the main body 10, so as to facilitate the realization of multi-person and multi-project detection of the microfluidic detection chip.

[0054] Furthermore, the detection area 23 is located on the side of the reagent tank 221 away from the center of the main body 10 and is connected to the reagent tank 221 through the second microchannel 26. When the microfluidic detection chip rotates, the functional reagents and samples in the reagent tank 221 can flow into the detection area 23 through the second microchannel 26. The functional reagents and samples mix and react in the detection area 23 and the detection area 23 can be optically detected by the optical path detection device on the detection instrument.

[0055] During the immunoassay of the sample, the magnetic bead antibody and the enzyme-labeled antibody can be added to the reagent tank 221 in sequence through the reagent injection position 222. The microfluidic detection chip is driven by the detection instrument to rotate and centrifuge, and the liquid in the reagent tank 221 is centrifugally transported to the detection area 23 for incubation reaction with the sample. After the reaction is completed, the magnetic beads in the detection area are cleaned. At this time, the detection instrument can add a magnetic field to the detection area 23 to adsorb the magnetic beads in the detection area 23, and then the cleaning liquid is injected into the detection area 23 through the reagent injection position 222 (see Figure 4As shown), the motor on the detection instrument is started to drive the microfluidic detection chip to rotate. At this time, the rotation speed of the microfluidic detection chip is less than 1500 rpm. After the cleaning liquid enters the detection area 23, the detection instrument adds upper and lower magnetic fields to the detection area 23 to move the magnetic beads, so that the magnetic beads and the cleaning liquid are fully mixed in the detection area 23 to achieve the cleaning effect. Then, part of the magnetic field is removed (only the magnetic field that fixes the magnetic beads is retained), and the motor continues to rotate, so that the cleaning liquid in the detection area 23 passes through the third microchannel 27 and enters the waste liquid storage area 24.

[0056] However, in the actual cleaning process, sometimes the waste liquid is not completely discharged into the waste liquid storage area 24. In order to completely discharge the liquid in the detection area 23 into the waste liquid storage area 24, the microfluidic detection chip can be emptied, such as Figure 5 As shown, during emptying, the microfluidic detection chip is first rotated at a low speed (less than 1500 rpm) by the motor of the detection instrument, and then rotated at a high speed (not less than 3500 rpm) by the motor of the detection instrument. In this way, high-speed centrifugation can be performed to transfer the liquid in the detection area 23 to the waste liquid storage area 24 for emptying. When the volume of liquid in the detection area 23 is small, the liquid in the detection area 23 will not flow from the third microchannel 27 to the waste liquid storage area 24 during centrifugation. When the volume of liquid in the detection area 23 is sufficient, the liquid in the detection area 23 will flow from the third microchannel 27 to the waste liquid storage area 24 during centrifugation. Optionally, a second microfluidic valve 28 is provided on the third microfluidic channel 27 in this embodiment, and the depth of the second microfluidic valve 28 is deeper than the depth of the third microfluidic channel 27. Specifically, the depth of the second microfluidic valve 28 is at least 1 mm deeper than the depth of the third microfluidic channel 27. Such a setting can prevent the backflow of liquid in the waste liquid storage area 24 to a certain extent.

[0057] In some embodiments, the third microchannel 27 has a raised portion 271 that protrudes toward the center of the main body 10, and the distance from the vertex of the raised portion 271 to the center of the main body 10 is not less than the distance from the end of the detection area 23 close to the center of the main body 10 to the center of the main body 10. That is to say, the top of the raised portion 271 is relatively close to the center of the main body 10, and the detection area 23 is relatively far from the center of the main body 10. In this way, when the magnetic beads in the detection area 23 are cleaned, the detection area 23 can be effectively maintained in the detection area 23 during the movement of the microfluidic detection chip, and is not easily affected by centrifugal force and flows from the third microchannel 27 into the waste liquid storage area 24. That is, the cleaning liquid can be well mixed with the magnetic beads in the detection area 23 to clean the magnetic beads, which can reduce the amount of cleaning liquid used during the magnetic bead cleaning process.

[0058] See again Figure 1 and Figure 2As shown, in some embodiments, the detection unit 20 further includes a buffer zone 29, and the third microfluidic channel 27 is connected between the detection area 23 and the buffer zone 29. The buffer zone 29 is connected to the waste liquid storage area 24 through the second connecting channel 201. By providing the buffer zone 29 between the waste liquid storage area 24 and the detection area 23, the liquid in the waste liquid storage area 24 can be prevented from flowing back into the detection area 23. It should be noted that the second connecting channel 201 here is a small channel, and the depth of the second connecting channel 201 is less than the depth of the waste liquid storage area 24 (the depth along the thickness direction of the body 10). Specifically, the depth of the second connecting channel 201 is at least 0.5 mm shallower than that of the waste liquid storage area 24. In this way, the liquid in the waste liquid storage area 24 can be prevented from flowing back.

[0059] Furthermore, the buffer zone 29 is connected to the waste liquid storage area 24 via at least two second connecting channels 201. For example, the buffer zone 29 and the waste liquid storage area 24 can be connected via two, three, or more second connecting channels 201. The accompanying drawings of this embodiment illustrate a situation where the buffer zone 29 and the waste liquid storage area 24 are connected via three second connecting channels 201. During actual use of the microfluidic detection chip, the second connecting channels 201 may occasionally become blocked. Since the buffer zone 29 and the waste liquid storage area 24 are connected via at least two second connecting channels 201 in this embodiment, even if a portion of the second connecting channels 201 is blocked, the liquid in the buffer zone 29 can be transported to the waste liquid storage area 24 via the other second connecting channels 201, resulting in a stable and reliable structure.

[0060] Furthermore, the detection unit 20 in this embodiment also includes a second air hole 203 and a second air groove 204. The second air hole 203 is connected to the buffer zone 29 through the second air groove 204. Through the second air hole 203 and the second air groove 204, the microfluidic detection chip can be emptied to avoid the formation of a high-pressure space on the microfluidic detection chip.

[0061] In order to effectively store waste liquids such as cleaning liquid, in this embodiment, the volume of the waste liquid storage area 24 is set to be larger than the volume of the buffer area 29, wherein the waste liquid storage area 24 is located on the side of the detection area 23 away from the reagent area 22, and the waste liquid storage area 24 includes a first edge 241 set close to the buffer area 29, and the first edge 241 includes a first end area 2411, a middle area 2412 and a second end area 2413. The first end area 2411, the middle area 2412 and the second end area 2413 are arranged along the periphery of the body 10. They are arranged in sequence, and the first end area 2411 is connected to the middle area 2412 by a first recessed area 2414, and the middle area 2412 is connected to the second end area 2413 by a second recessed area 2415. The first recessed area 2414 and the second recessed area 2415 are grooved in the direction away from the buffer area 29, and the first end area 2411 and the second end area 2413 are protruded in the direction close to the buffer area 29. The connecting position of the second connecting channel 201 and the waste liquid storage area 24 is located in the middle area 2412. In this way, when the microfluidic detection chip rotates, the first end area 2411 and the second end area 2413 that are protruded toward the buffer zone 29 can guide the liquid in the waste liquid storage area 24, and can guide the liquid in the waste liquid storage area 24 toward the inside of the waste liquid storage area 24. At the same time, the first recessed area 2414 and the second recessed area 2415 located at both ends of the middle area 2412 can respectively block the liquid flowing from the first end area 2411 and the second end area 2413, thereby preventing the liquid in the waste liquid storage area 24 from flowing toward the middle area 2412, and further preventing the liquid in the waste liquid storage area 24 from flowing to the second connecting channel 201, and further preventing the liquid in the waste liquid storage area 24 from flowing back into the buffer zone 29.

[0062] Specifically, the waste liquid storage area 24 in this embodiment also includes a second edge 242, which is located on the side of the waste liquid storage area 24 close to the outer edge of the main body 10. The second edge 242 is an arc-shaped edge, which is parallel to the outer edge of the main body 10, which facilitates the miniaturization design of the main body 10 and can reduce the production cost of the microfluidic detection chip.

[0063] During actual use, under the action of centrifugal force, the magnetic beads in the detection area 23 will occasionally be transported to the waste liquid storage area 24 or even thrown out in the direction close to the outer edge of the main body 10. When the magnetic beads are thrown out of the waste liquid storage area 24 under the action of centrifugal force, the magnetic beads will be clamped between the middle layer and the main body 10. When the centrifugal force is large enough, the magnetic beads will move between the middle layer and the main body 10 and lift the middle layer from the main body 10, causing leakage. To this end, the detection unit 20 in this embodiment also includes a stop groove 202, which is arranged on the side of the waste liquid storage area 24 away from the detection area 23. When the magnetic beads are thrown out of the waste liquid storage area 24 under the action of centrifugal force, they can be accommodated in the stop groove 202 located outside the waste liquid storage area 24, thereby preventing the magnetic beads from continuing to move toward the outer edge of the main body 10 and lifting the middle layer, ultimately achieving the effect of preventing leakage.

[0064] Optionally, the stop groove 202 in this embodiment extends along the circumference of the main body 10, and the length of the stop groove 202 is not less than the length of the waste liquid storage area 24 along the circumference of the main body 10. That is to say, the length of the stop groove 202 along the circumference of the main body 10 is equal to or greater than the length of the waste liquid storage area 24 along the circumference of the main body 10, which is convenient for stopping the magnetic beads flowing out of the waste liquid storage area 24 and accommodating them in the stop groove 202. The magnetic beads will not lift the middle layer from the main body 10, and the microfluidic detection chip is not prone to leakage.

[0065] From the above description, it can be seen that the above-mentioned embodiments of the present invention achieve the following technical effects: Since the microfluidic detection chip of the present invention is provided with a plurality of detection units, the structure of each detection unit is simple, and it is only necessary to control the rotation speed and direction of the detection instrument to achieve sample separation, achieve whole blood sampling, and achieve the sequential release of functional reagents, samples, etc. At the same time, the microfluidic detection chip can realize multi-person multi-item detection, has a simple structure, and can reduce the production and manufacturing costs and detection costs of the microfluidic detection chip. In addition, the microfluidic detection chip of the present invention can save the amount of cleaning fluid used and is not prone to leakage problems.

[0066] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used herein to describe the spatial positional relationship of a device or feature to other devices or features as shown in the figures. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figures. For example, if the device in the drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0067] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the present invention.

[0068] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A microfluidic detection chip, characterized in that: The invention comprises a sheet-like body (10) and a plurality of detection units (20) arranged on the body (10), wherein the plurality of detection units (20) are radially arranged on the body (10), and the detection units (20) comprise: A sample area (21), the sample area (21) is at least used for injecting a sample; a reagent area (22), the reagent area (22) is used for injecting a functional reagent, the reagent area (22) includes a reagent tank (221), and the reagent tank (221) is connected to the sample area (21) through a first microchannel (25); a detection zone (23), wherein the detection zone (23) is used for performing a mixing reaction of a functional reagent and a sample, and the detection zone (23) is connected to the reagent zone (22) via a second microfluidic channel (26); and A waste liquid storage area (24), the waste liquid storage area (24) is used to hold functional reagents and samples after the reaction, and the waste liquid storage area (24) is connected to the detection area (23) through a third microchannel (27); The first microchannel (25), the second microchannel (26) and the third microchannel (27) are at least used to realize the sequential release of functional reagents and samples in the microfluidic detection chip.

2. The microfluidic detection chip according to claim 1, characterized in that: The sample area (21) includes a sample separation unit, and the sample separation unit includes: An injection slot (211), the injection slot (211) is used for sample injection; a plasma / serum tank (212), the plasma / serum tank (212) being connected to the injection tank (211), and the plasma / serum tank (212) being used for temporarily storing the separated plasma / serum; a blood cell tank (213), the blood cell tank (213) being located on a side of the plasma / serum tank (212) away from the injection tank (211) and being in communication with the plasma / serum tank (212), the blood cell tank (213) being used for temporarily storing the separated blood cells; and A storage tank (214), the storage tank (214) is connected to the plasma / serum tank (212), and the storage tank (214) is used to quantify the sample injection amount.

3. The microfluidic detection chip according to claim 2, characterized in that: A connection area (215) is provided on a side of the plasma / serum tank (212) away from the injection tank (211), and the first microchannel (25) is connected to the plasma / serum tank (212) through the connection area (215); The sample separation unit further includes a first connecting channel (216), the blood cell tank (213) and the connecting area (215) are connected via the first connecting channel (216), and the depths of the first connecting channel (216) and the first microchannel (25) are both less than the depth of the connecting area (215).

4. The microfluidic detection chip according to claim 1, characterized in that: A first microfluidic valve is provided on the first microfluidic channel (25), and the depth of the first microfluidic valve is at least 1 mm deeper than the depth of the first microfluidic channel (25); and / or, A second microfluidic valve (28) is provided on the third microfluidic channel (27), and the depth of the second microfluidic valve (28) is at least 1 mm deeper than the depth of the second microfluidic channel (26).

5. The microfluidic detection chip according to claim 1, characterized in that: The detection unit (20) further includes a buffer zone (29), the third microchannel (27) is connected between the detection zone (23) and the buffer zone (29), and the buffer zone (29) is connected to the waste liquid storage zone (24) via a second connecting channel (201).

6. The microfluidic detection chip according to claim 5, characterized in that: The buffer area (29) is connected to the waste liquid storage area (24) through at least two second connecting channels (201).

7. The microfluidic detection chip according to claim 5, characterized in that: The volume of the waste liquid storage area (24) is greater than the volume of the buffer area (29), wherein The waste liquid storage area (24) is located on a side of the detection area (23) away from the reagent area (22), and the waste liquid storage area (24) includes a first edge (241) arranged near the buffer area (29), and the first edge (241) includes a first end area (2411), a middle area (2412) and a second end area (2413), and the first end area (2411), the middle area (2412) and the second end area (2413) are arranged in sequence along the circumference of the body (10), and the first end area (2411) and the middle area (2412) are arranged in a circumferential direction of the body (10). 412) are connected by a first recessed area (2414), the middle area (2412) and the second end area (2413) are connected by a second recessed area (2415), the first recessed area (2414) and the second recessed area (2415) are recessed in a direction away from the buffer area (29), the first end area (2411) and the second end area (2413) are protruded in a direction close to the buffer area (29), and the connection position of the second connecting channel (201) and the waste liquid storage area (24) is located in the middle area (2412).

8. The microfluidic detection chip according to any one of claims 1 to 7, characterized in that: The detection unit (20) further comprises a stop groove (202), wherein the stop groove (202) is arranged on a side of the waste liquid storage area (24) away from the detection area (23).

9. The microfluidic detection chip according to claim 8, characterized in that: The stopping groove (202) extends along the circumference of the body (10), and the length of the stopping groove (202) is not less than the length of the waste liquid storage area (24) along the circumference of the body (10).

10. The microfluidic detection chip according to any one of claims 1 to 7, characterized in that: The third microchannel (27) has a protrusion (271) protruding toward the center of the body (10), and the distance from the vertex of the protrusion (271) to the center of the body (10) is not less than the distance from one end of the detection area (23) close to the center of the body (10) to the center of the body (10).

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