Foliar fertilizer containing protein polypeptide and preparation method thereof

Abstract

The present invention relates to a foliar fertilizer containing protein polypeptide and a preparation method thereof, and relates to the technical field of fertilizers. The raw materials of the foliar fertilizer of the present invention include the following components by mass: 8-12.8 parts of protein polypeptide, 25-30 parts of mineral source potassium humate, 15-20 parts of urea, 2-12 parts of potassium dihydrogen phosphate, 2-4 parts of zinc sulfate, 1-5 parts of magnesium sulfate, 2-6 parts of ferrous sulfate, 1-1.5 parts of copper sulfate, 5-8 parts of gamma-aminobutyric acid, 2-3 parts of sodium citrate and 55-75 parts of water. The formula of the present invention is scientific and reasonable, contains a variety of nutrient elements required for crop growth, fertilizer nutrients are well absorbed and have high utilization rate, can provide suitable nutritional conditions for crop growth, the foliar fertilizer can improve crop stress resistance, ensure the yield-increasing effect, and has the characteristics of simple preparation process, low cost, high fertilizer efficiency, and green environmental protection, and is suitable for large-scale promotion and application in the agricultural field.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, in particular to a foliar fertilizer containing protein polypeptides and a preparation method thereof. Background Art

[0002] The problem of decreasing cultivated land area and slow growth of agricultural output has become increasingly prominent. In order to increase crop yields and economic benefits, farmers apply a large amount of chemical fertilizers, but long-term unreasonable fertilization has caused great damage to soil structure and ecological environment. The problems of soil compaction and decreased fertility are prominent, which seriously restricts the increase of crop yields.

[0003] Normally, plants mainly absorb nutrients from the soil through their roots to supply crop growth and development. In addition to the roots, the stems and leaves of plants can also absorb various nutrients. Compared with the traditional rhizosphere fertilization method, spraying foliar fertilizer can avoid the loss and conversion of nutrients in the soil, directly provide nutrition for plants, and improve the problems such as soil compaction caused by the application of rhizosphere fertilizer. As a liquid fertilizer that can enhance plant nutrition, foliar fertilizer has the characteristics of small dosage, high utilization rate, and fast absorption speed, and has extremely high promotion and application value. However, the high cost and complex process of foliar fertilizer preparation limit its promotion and application. At the same time, there are problems in the absorption and transfer of some nutrients in the fertilizer during the fertilization process, resulting in unsatisfactory spraying effect. Summary of the invention

[0004] The purpose of the present invention is to provide a foliar fertilizer containing protein polypeptide and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art and ensure a good yield-increasing effect of foliar fertilization on crops.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention is to provide a foliar fertilizer containing protein polypeptide, the raw materials comprising the following components in parts by weight:

[0007] 8-12.8 parts of protein polypeptide, 25-30 parts of mineral potassium humate, 15-20 parts of urea, 2-12 parts of potassium dihydrogen phosphate, 2-4 parts of zinc sulfate, 1-5 parts of magnesium sulfate, 2-6 parts of ferrous sulfate, 1-1.5 parts of copper sulfate, 5-8 parts of gamma-aminobutyric acid, 2-3 parts of sodium citrate and 55-75 parts of water.

[0008] Furthermore, the mass ratio of the protein polypeptide to γ-aminobutyric acid is 1.6:1.

[0009] As a further preferred embodiment of the present invention, the foliar fertilizer raw material containing protein polypeptides comprises the following components in parts by weight:

[0010] 8 parts of protein polypeptide, 25 parts of mineral potassium humate, 18 parts of urea, 8 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 2 parts of magnesium sulfate, 5 parts of ferrous sulfate, 1.3 parts of copper sulfate, 5 parts of gamma-aminobutyric acid, 2.5 parts of sodium citrate and 65 parts of water.

[0011] Furthermore, the protein polypeptide is prepared by the following steps:

[0012] The fish meat is ground and then papain is added for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, the supernatant is taken by centrifugation, and filtered to obtain the protein polypeptide.

[0013] Furthermore, the temperature of the enzymatic hydrolysis is 25-28°C, and the time is 1 to 1.5 hours.

[0014] Furthermore, the added amount of papain is 1.5-2‰ of the fish meat mass.

[0015] Furthermore, the mineral source potassium humate has a potassium oxide content of ≥10wt%, a humic acid content of ≥55wt%, and a fulvic acid content of ≥50wt%.

[0016] The second technical solution of the present invention is to provide a method for preparing the above-mentioned foliar fertilizer, comprising the following steps:

[0017] The raw materials are mixed according to a mass ratio, and stirred at room temperature (0.5-1h) to obtain the foliar fertilizer containing protein polypeptides.

[0018] The third technical solution of the present invention is to provide the application of the above-mentioned foliar fertilizer in leafy vegetable crops and melon and fruit crops.

[0019] The leafy vegetable crops include lettuce and cabbage; the melon and fruit crops include cucumber.

[0020] Furthermore, the application method comprises the following steps: diluting the foliar fertilizer containing the protein polypeptide by 200-300 times with water, and spraying the fertilizer on the leaves 1-3 times during the crop growth period at an application rate of 250-500 g / mu.

[0021] The animal-derived protein polypeptide in the fertilizer of the present invention has obvious physiological activity on crops, can improve the stress resistance of crops, avoid the problem of reduced production caused by pests and diseases during crop growth, and ensure the effect of increasing crop production.

[0022] The γ-aminobutyric acid and protein polypeptide in the fertilizer can ensure the stability of fertilizer effect, ensure the absorption and utilization rate of trace elements, and promote crop growth. At the same time, the compounding of γ-aminobutyric acid and sodium citrate can further enhance the absorption and conversion of nutrients by crops, effectively improving the yield-increasing effect. The present invention has found through research that when the protein polypeptide and γ-aminobutyric acid are compounded at a mass ratio of 1.6:1, the yield-increasing effect is most significant.

[0023] The present invention discloses the following technical effects:

[0024] The foliar fertilizer of the present invention has a scientific and reasonable formula, contains multiple nutrient elements required for crop growth, has good absorption of fertilizer nutrients and high utilization rate, and can provide suitable nutritional conditions for crop growth; the foliar fertilizer of the present invention can improve crop stress resistance and ensure the yield-increasing effect, and at the same time has the characteristics of simple preparation process, low cost, high fertilizer efficiency, and green environmental protection, and is suitable for large-scale promotion and application in the agricultural field. DETAILED DESCRIPTION

[0025] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0026] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0027] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0028] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0029] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0030] The room temperature in the present invention is 25° C. unless otherwise specified.

[0031] The mineral source potassium fulvic acid used in the embodiment of the present invention has a potassium oxide content of ≥10wt%, a humic acid content of ≥55wt%, and a fulvic acid content of ≥50wt%.

[0032] Example 1

[0033] A foliar fertilizer containing protein polypeptide, the raw materials are the following components in parts by weight:

[0034] 8 parts of protein polypeptide, 25 parts of mineral potassium humate, 18 parts of urea, 8 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 2 parts of magnesium sulfate, 5 parts of ferrous sulfate, 1.3 parts of copper sulfate, 5 parts of gamma-aminobutyric acid, 2.5 parts of sodium citrate and 65 parts of water.

[0035] The steps for preparing the protein polypeptide are as follows:

[0036] After the fish meat is ground, 1.5‰ of papain by weight of the fish meat is added, and enzymolysis is carried out at 26°C for 1.5 hours. After the enzymolysis is completed, the enzyme is inactivated at 95°C for 15 minutes, and the supernatant is collected by centrifugation and filtered to obtain protein polypeptides.

[0037] Preparation of foliar fertilizer containing protein peptides:

[0038] The above raw materials are mixed according to the mass ratio, and stirred at room temperature for 0.6 h to obtain the foliar fertilizer containing protein polypeptide.

[0039] Example 2

[0040] A foliar fertilizer containing protein polypeptide, the raw materials are the following components in parts by weight:

[0041] 8 parts of protein polypeptide, 25 parts of mineral potassium humate, 20 parts of urea, 3 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 1 part of magnesium sulfate, 5 parts of ferrous sulfate, 1.2 parts of copper sulfate, 6 parts of gamma-aminobutyric acid, 2 parts of sodium citrate and 75 parts of water.

[0042] The steps for preparing the protein polypeptide are as follows:

[0043] After the fish meat is ground, 2‰ of papain by weight of the fish meat is added, and enzymolysis is carried out at 25°C for 1.2 hours. After the enzymolysis is completed, the enzyme is inactivated at 95°C for 20 minutes, and the supernatant is collected by centrifugation and filtered to obtain protein polypeptides.

[0044] Preparation of foliar fertilizer containing protein peptides:

[0045] The above raw materials are mixed according to the mass ratio, and stirred at room temperature for 0.5 h to obtain the foliar fertilizer containing protein polypeptide.

[0046] Example 3

[0047] A foliar fertilizer containing protein polypeptide, the raw materials are the following components in parts by weight:

[0048] 10 parts of protein polypeptide, 28 parts of mineral potassium humate, 18 parts of urea, 12 parts of potassium dihydrogen phosphate, 2 parts of zinc sulfate, 5 parts of magnesium sulfate, 2 parts of ferrous sulfate, 1 part of copper sulfate, 8 parts of gamma-aminobutyric acid, 3 parts of sodium citrate and 55 parts of water.

[0049] The steps for preparing the protein polypeptide are as follows:

[0050] The fish meat was ground and 1.8‰ of papain by weight of the fish meat was added, and enzymolysis was performed at 26°C for 1 hour. After the enzymolysis was completed, the enzyme was inactivated at 95°C for 20 minutes, and the supernatant was collected by centrifugation and filtered to obtain protein polypeptides.

[0051] Preparation of foliar fertilizer containing protein peptides:

[0052] The above raw materials are mixed according to the mass ratio, and stirred at room temperature for 1 hour to obtain the foliar fertilizer containing protein polypeptide.

[0053] Example 4

[0054] A foliar fertilizer containing protein polypeptide, the raw materials are the following components in parts by weight:

[0055] 12.8 parts of protein polypeptide, 25 parts of mineral potassium humate, 20 parts of urea, 3 parts of potassium dihydrogen phosphate, 4 parts of zinc sulfate, 2 parts of magnesium sulfate, 6 parts of ferrous sulfate, 1.3 parts of copper sulfate, 6 parts of gamma-aminobutyric acid, 2.5 parts of sodium citrate and 65 parts of water.

[0056] The steps for preparing the protein polypeptide are as follows:

[0057] After the fish meat is ground, 2‰ of papain by weight of the fish meat is added, and enzymolysis is carried out at 25°C for 1.2 hours. After the enzymolysis is completed, the enzyme is inactivated at 95°C for 20 minutes, and the supernatant is collected by centrifugation and filtered to obtain protein polypeptides.

[0058] Preparation of foliar fertilizer containing protein peptides:

[0059] The above raw materials are mixed according to the mass ratio, and stirred at room temperature for 1 hour to obtain the foliar fertilizer containing protein polypeptide.

[0060] Comparative Example 1

[0061] The only difference from Example 1 is that no protein polypeptide is added, and the protein polypeptide is replaced by an equal mass portion of mineral source potassium humate.

[0062] Comparative Example 2

[0063] The only difference from Example 1 is that the added amounts of protein polypeptide and γ-aminobutyric acid are adjusted.

[0064] The quality ratio of raw material components is as follows:

[0065] 9 parts of protein polypeptide, 25 parts of mineral potassium humate, 18 parts of urea, 8 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 2 parts of magnesium sulfate, 5 parts of ferrous sulfate, 1.3 parts of copper sulfate, 4 parts of gamma-aminobutyric acid, 2.5 parts of sodium citrate and 65 parts of water.

[0066] Comparative Example 3

[0067] The only difference from Example 1 is that no sodium citrate is added.

[0068] Effect verification example:

[0069] The foliar fertilizer prepared in Example 1-4 and Comparative Example 1-3 was diluted 300 times with water, and then sprayed once on the leaves of lettuce (head lettuce) at the seedling stage at an application rate of 250 g per mu. The appearance and yield of the lettuce are shown in Table 1:

[0070] Table 1

[0071] Appearance Yield (kg / mu) Example 1 Green leaves, vigorous growth 3165 Example 2 Green leaves, vigorous growth 3115 Example 3 Green leaves, vigorous growth 3128 Example 4 Green leaves, vigorous growth 3106 Comparative Example 1 The leaves are relatively green and grow well. 2858 Comparative Example 2 The leaves are relatively green and grow well. 3054 Comparative Example 3 The leaves are relatively green and grow well. 2955

[0072] The foliar fertilizer prepared in Examples 1-4 and Comparative Examples 1-3 was diluted 250 times with water, and sprayed once on the leaves of Chinese cabbage at the seedling stage, middle stage, and late stage according to the application amount of 350 g of foliar fertilizer per mu. The appearance and yield of Chinese cabbage are shown in Table 2:

[0073] Table 2

[0074] Appearance Yield (kg / mu) Example 1 Green leaves, vigorous growth 2658 Example 2 Green leaves, vigorous growth 2545 Example 3 Green leaves, vigorous growth 2586 Example 4 Green leaves, vigorous growth 2520 Comparative Example 1 The leaves are relatively green and grow well. 2005 Comparative Example 2 The leaves are relatively green and grow well. 2258 Comparative Example 3 The leaves are relatively green and grow well. 2106

[0075] The foliar fertilizer prepared in Examples 1-4 and Comparative Examples 1-3 was diluted 300 times with water, and 400 g of foliar fertilizer was applied per mu. The foliar fertilizer was sprayed once on the leaves of cucumbers (autumn cucumbers) at the seedling stage and young melon stage. The appearance and yield of the cucumbers are shown in Table 3:

[0076] Table 3

[0077] Appearance Yield (kg / mu) Example 1 The leaves are green and the fruits grow vigorously 7650 Example 2 The leaves are green and the fruits grow vigorously 7535 Example 3 The leaves are green and the fruits grow vigorously 7510 Example 4 The leaves are green and the fruits grow vigorously 7550 Comparative Example 1 The leaves are relatively green and the fruits grow well. 7106 Comparative Example 2 The leaves are relatively green and the fruits grow well. 7365 Comparative Example 3 The leaves are relatively green and the fruits grow well. 7250

[0078] The foliar fertilizer of the present invention can significantly promote the growth of leafy vegetables, and also has a growth-promoting effect on fruits and vegetables, and has important economic value in the agricultural field. The foliar fertilizer of the present invention has significant fertilizer effect, good growth trend of crops, excellent quality, and significant economic benefits.

[0079] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A foliar fertilizer containing protein polypeptide for leafy vegetables or melon and fruit crops, characterized in that: The raw materials include the following components in parts by weight: 8-12.8 parts of protein polypeptide, 25-30 parts of mineral potassium humate, 15-20 parts of urea, 2-12 parts of potassium dihydrogen phosphate, 2-4 parts of zinc sulfate, 1-5 parts of magnesium sulfate, 2-6 parts of ferrous sulfate, 1-1.5 parts of copper sulfate, 5-8 parts of gamma-aminobutyric acid, 2-3 parts of sodium citrate and 55-75 parts of water; The leafy vegetable crops are lettuce or cabbage, and the melon and fruit crops are cucumbers; The mass ratio of the protein polypeptide to γ-aminobutyric acid is 1.6:1; The protein polypeptide is prepared by the following steps: grinding the fish meat, adding papain for enzymolysis, killing the enzyme after the enzymolysis is completed, centrifuging to obtain the supernatant, filtering to obtain the protein polypeptide; The enzymatic hydrolysis temperature is 25-28°C and the time is 1-1.5h; The mineral source potassium humate has a potassium oxide content of ≥10wt%, a humic acid content of ≥55wt%, and a fulvic acid content of ≥50wt%.

2. Foliar fertilizer according to claim 1, characterized in that, The raw materials include the following components in parts by weight: 8 parts of protein polypeptide, 25 parts of mineral potassium humate, 18 parts of urea, 8 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 2 parts of magnesium sulfate, 5 parts of ferrous sulfate, 1.3 parts of copper sulfate, 5 parts of gamma-aminobutyric acid, 2.5 parts of sodium citrate and 65 parts of water.

3. Foliar fertilizer according to claim 1, characterized in that, The added amount of papain is 1.5-2‰ of the fish meat mass.

4. The method for preparing the foliar fertilizer according to any one of claims 1 to 3, wherein The method comprises the following steps: mixing the raw materials according to a mass ratio to obtain the foliar fertilizer containing the protein polypeptide.

5. The use of the foliar fertilizer according to any one of claims 1 to 3 in leafy vegetable crops and melon and fruit crops; characterized in that: The leafy vegetable crops are lettuce or cabbage, and the melon and fruit crops are cucumbers; The application method comprises the following steps: diluting the foliar fertilizer containing the protein polypeptide by 200-300 times with water, and spraying the fertilizer on the leaves 1-3 times during the crop growth period at an application rate of 250-500 g / mu.