A sex attractant composition for preventing or monitoring mealybugs

Abstract

The invention provides a sex attractant composition for preventing, controlling or monitoring hibiscus mealybugs. By using the sex pheromone of the hibiscus mealybugs in combination with a synergist and selecting the appropriate type and proportion of the synergist, a sex attractant for the hibiscus mealybugs with high attraction, long-lasting effect, strong specificity and low cost is developed, which solves the problems of prediction, forecasting and prevention and control of the hibiscus mealybugs, improves the comprehensive prevention and control effect, saves the use cost, and can be applied to the prediction, forecasting, mass trapping and mating interference of the hibiscus mealybugs, which is of great significance for the comprehensive control of the hibiscus mealybugs.

Description

Technical Field

[0001] The invention belongs to the field of plant disease and insect pest control, and in particular, relates to a sex attractant composition for controlling or monitoring hibiscus mealybugs. Background Art

[0002] Phenacoccus solenopsis Tinsley, also known as cotton mealybug, belongs to the order Hemiptera, family Pseudococcidae, genus Phenacoccus. Phenacoccus was originally native to North America and invaded my country in 2008. So far, it has invaded dozens of provinces and cities in my country, with distribution in both the north and south. It has strong adaptability and has become an important invasive pest in agriculture. Phenacoccus solenopsis Tinsley is polyphagous and has a wide range of hosts, including food crops, vegetable crops, ornamental flowers, garden plants and cash crops, causing serious economic losses every year. Due to the characteristics of small individuals, strong secretiveness, high reproductive capacity, overlapping generations, strong stress resistance and wax shell on the back of the body, Phenacoccus solenopsis Tinsley has obvious ecological advantages, and chemical control is difficult to achieve ideal results. Therefore, it is urgent to develop "green control" technology for hibiscus mealybugs, and sex pheromone technology has become an ideal choice.

[0003] Long-term and large-scale use of pesticides not only causes environmental pollution, but also causes insects to develop resistance to pesticides. Compared with chemical control, sex pheromone technology is increasingly used in integrated control such as insect monitoring and large-scale trapping. Therefore, it is particularly urgent to develop sex attractant technology for Phenacoccus hibiscus.

[0004] At present, the main component of the sex pheromone of the hibiscus mealybug is (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, which has a strong attraction to the male hibiscus mealybug. However, due to its low persistence, it has a high replacement cost and is difficult to promote on a large scale. Therefore, it is urgent to find a sex attractant for the hibiscus mealybug that has good luring effect, long persistence, low cost, and is suitable for large-scale promotion. Summary of the invention

[0005] To solve the above problems, the present invention provides a sex attractant composition for controlling or monitoring hibiscus mealybug. By combining the sex pheromone of hibiscus mealybug with a synergist, a sex attractant for hibiscus mealybug with high attraction and long lasting effect is developed, which solves the problems of prediction, forecasting and prevention and control of hibiscus mealybug, improves the comprehensive prevention and control effect, and saves the use cost.

[0006] On the one hand, the present invention provides a sex attractant composition for Phenacoccus sempervirens, comprising a sex pheromone and a synergist, wherein the sex pheromone is (R)-isoprene acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, and the synergist is any one or more of myrcene, α-pinene, β-pinene, cis-3-hexenol or linalool.

[0007] Furthermore, the weight ratio of the sex pheromone to the synergist is 1:0.01-2.0.

[0008] Through a large number of experiments, the present invention finds that the hibiscus mealybug sex pheromone (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester can significantly improve the control effect of the hibiscus mealybug after being combined with a synergist in a certain manner.

[0009] Among them, the combination of sex pheromones and synergists has a very important influence on the effect of attracting hibiscus mealybugs. It not only involves the selection of synergist types, but also involves the matching of the proportional relationship between sex pheromones and various synergists. A slight adjustment in the proportional relationship will directly affect the prevention and control effect and reduce the synergistic effect. Therefore, the types of synergists and their mutual proportional relationships must be strictly controlled to obtain good synergistic effects.

[0010] Studies have shown that when the sex pheromone (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester is used in combination with only one synergist, it has no effect on the effect of attracting the hibiscus mealybug. However, the combination of sex pheromone with multiple synergists and in a specific proportion can significantly improve the effect of attracting the hibiscus mealybug.

[0011] In some embodiments, the weight ratio of the sex pheromone to the synergist is 1:0.1-0.5.

[0012] In some embodiments, the synergist is α-pinene and β-pinene.

[0013] Furthermore, the synergist is α-pinene and β-pinene, and the weight ratio of (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, α-pinene and β-pinene is (5-10):(1-21):1.

[0014] Furthermore, the weight ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, α-pinene and β-pinene is 10:21:1 or 5:1:1.

[0015] In some embodiments, the synergist is myrcene, α-pinene and β-pinene.

[0016] Furthermore, the weight ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene and β-pinene is 20:(2-4):1:(1-2).

[0017] Furthermore, the weight ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene and β-pinene is 20:4:1:1 or 20:2:1:2.

[0018] In some embodiments, the synergist is myrcene, α-pinene, β-pinene and cis-3-hexenol.

[0019] Furthermore, the weight ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene and β-pinene is 20:(2-4):1:(1-2).

[0020] Furthermore, the weight ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is 40:12:2:1:1 or 20:2:2:1:1.

[0021] In some embodiments, the synergist is myrcene, α-pinene, β-pinene and linalool.

[0022] Furthermore, the weight ratio of (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is (20-40):(2-12):2:1:1.

[0023] Furthermore, the weight ratio of (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and linalool is 20:2:1:2:1.

[0024] In some embodiments, the synergist is myrcene, α-pinene, β-pinene, cis-3-hexenol and linalool.

[0025] Furthermore, the synergist is myrcene, α-pinene, β-pinene, cis-3-hexenol and linalool, wherein, by mass fraction, myrcene is 1 to 99 parts, α-pinene is 10 to 40 parts, β-pinene is 5 to 90 parts, cis-3-hexenol is 1 to 95 parts and linalool is 1 to 9 parts.

[0026] In some embodiments, the synergist comprises 20 to 40 parts of myrcene, 10 to 20 parts of α-pinene, 20 to 40 parts of β-pinene, 20 to 60 parts of cis-3-hexenol and 2 to 6 parts of linalool.

[0027] In some embodiments, the weight percentage of the hibiscus mealybug sex pheromone is 100 parts.

[0028] Furthermore, the weight ratio of (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is (20-40):(2-12):2:1:1.

[0029] Furthermore, the weight ratio of (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and linalool is 40:8:3:6:9:1 or 40:8:2:4:2:1.

[0030] In some embodiments, the hibiscus mealybug sex attractant composition includes, by mass fraction, 200 parts of hibiscus mealybug sex pheromone, 40 parts of myrcene, 15 parts of α-pinene, 30 parts of β-pinene, 45 parts of cis-3-hexenol and 5 parts of linalool.

[0031] In some embodiments, the (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester is the sex pheromone of Phenacoccus hibiscus L., which has a chemical purity of 90% and an optical purity of 92%, and is artificially synthesized.

[0032] On the other hand, the present invention provides an attractant core for controlling Phenacoccus hibiscus, wherein the attractant core contains a Phenacoccus hibiscus sex attractant composition.

[0033] In some embodiments, the attractant is prepared by the following method: including applying a synergist to an attractant containing the sex pheromone of the hibiscus mealybug; or mixing the synergist with the sex pheromone of the hibiscus mealybug and then adding the mixture to a blank attractant; or applying the synergist to a blank attractant, and then using the attractant containing the synergist in combination with the sex pheromone of the hibiscus mealybug.

[0034] In another aspect, the present invention provides a method for controlling or monitoring Phenacoccus sibiricus, comprising the following steps:

[0035] 1) preparing an attractant core containing the above-mentioned Phenacoccus hibiscus sexual attractant composition;

[0036] 2) Place the bait core in the trap and place it in the place where the hibiscus mealybug needs to be controlled or monitored.

[0037] In some embodiments, the place where the hibiscus mealybugs need to be controlled or monitored is a field.

[0038] In some methods, the synergist and the sex pheromone of the hibiscus mealybug can be separately prepared into attractants, and then the synergist attractant and the sex pheromone attractant of the hibiscus mealybug can be used together.

[0039] In some methods, the synergist and the sex pheromone can be first mixed according to a specific gravity and then added to the bait core to make a highly effective sex attractant bait core.

[0040] Preferably, the synergist can also be added to the bait core that already contains the sex pheromone of Phenacoccus hibiscus.

[0041] In some embodiments, the hibiscus mealybug sex attractant composition can be prepared by dissolving the sex pheromone and the synergist in n-hexane in a certain ratio to prepare a 1-100 μg / μL n-hexane solution.

[0042] In some embodiments, in step 2), the lure core containing the sex attractant composition is placed in a trap, each lure core contains 50 to 2000 μg of a highly effective sex attractant for the hibiscus mealybug, and is placed in a plot where the hibiscus mealybug is infested.

[0043] In some embodiments, the lure core may be a rubber head lure core or a PVC tube, and the sex attractant composition may volatilize naturally in the lure core.

[0044] In some embodiments, the lure is a commonly used lure on the market, and both lures (rubber head lures or PVC tubes) have a sustained release effect on the release of sex pheromones.

[0045] In some embodiments, the decoy core is purchased from Ningbo Newcom Biotechnology Co., Ltd.

[0046] In some embodiments, the trap is any one of a sticky insect board trap, a boat-shaped trap, a triangular trap, a barrel-shaped trap, a basin-shaped trap, etc., as long as the hibiscus mealybug can be lured through the bait core and captured.

[0047] Furthermore, the dosage of the composition for controlling Phenacoccus hibiscus is 0.1-15 μg / m 2 The dosage of the composition for monitoring Phenacoccus hibiscus was 0.1-5 μg / m 2 .

[0048] In another aspect, the present invention provides use of the above-mentioned Phenacoccus hibiscus sex attractant composition for preparing a preparation or attractant core for controlling or monitoring Phenacoccus hibiscus.

[0049] The high-efficiency sex attractant for Phenacoccus hibiscus provided by the present invention can be applied in various forms, such as being prepared into a preparation form, or being prepared into a attractant core form, etc. As long as the sex pheromone of Phenacoccus hibiscus and its synergist can produce a synergistic effect, the trapping activity can be improved and the use cost can be reduced.

[0050] The present invention also provides a method for preserving a high-efficiency sex attractant composition for hibiscus mealybugs. The prepared sex attractant core is placed in an aluminum foil bag and refrigerated (-20° C.). The validity period of the sex attractant core for hibiscus mealybugs is about 60 days, and the mixture can be prepared and used immediately during application.

[0051] The high-efficiency attractant composition for Phenacoccus sangifera provided by the present invention and the application method thereof have the following beneficial effects:

[0052] 1. The raw materials of the synergist are easily available and the preparation of the composition is simple;

[0053] 2. Greatly reduce the application amount of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester, reduce costs, and have a longer lasting effect and better stability;

[0054] 3. It has strong trapping ability and specificity, and is highly effective against Phenacoccus hibiscus;

[0055] 4. Environmentally friendly and has no impact on non-target organisms;

[0056] 5. It can be used for prediction and forecast of the hibiscus mealybug pest, mass trapping, and mating interference, which is of great significance for the comprehensive management of the hibiscus mealybug pest. DETAILED DESCRIPTION

[0057] The present invention will be further described in detail below in conjunction with the examples. It should be pointed out that the examples described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on the present invention.

[0058] In the following examples, the hibiscus mealybug sex pheromone (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester is the hibiscus mealybug sex pheromone, which has a chemical purity of 90% and an optical purity of 92%. It is synthesized in the laboratory, and the synthesis method is referenced to the synthesis method of the hibiscus mealybug sex pheromone in Zhang A, Nie J (2005) Enantioselective synthesis of the female sexpheromone of the pink hibiscus mealybug, Maconellicoccus hirsutus. J Agric Food Chem 53: 2451–2455; myrcene was purchased from Aladdin Reagent Co., Ltd.; α-pinene was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; β-pinene was purchased from Aladdin Reagent Co., Ltd.; cis-3-hexenol was purchased from Sinopharm Chemical Reagent Co., Ltd.; and linalool was purchased from Aladdin Reagent Co., Ltd.

[0059] Example 1 Combination of Phenacoccus sibiricus Sex Pheromone and a Synergist

[0060] The sex pheromone of Phenacoccus hibiscus was dissolved in n-hexane, and the required amount was added to the lure (rubber head lure or PVC tube. After the solvent evaporated (one hour), the required amount of synergist (dissolved in n-hexane) was added to the lure (purchased from Ningbo Newcom Biotechnology Co., Ltd.). The formula and content of the sex attractant composition contained in each lure are shown in Table 1. During field use, the traps (sticky insect boards) were placed at intervals of 5 to 10 meters per trap. The plots where Phenacoccus hibiscus was more serious were selected as the test plots, and 6 replicates were set up in each plot, and sex attractants of different formulations were randomly placed. The composition is used, and the number of male insects trapped is continuously investigated and counted for 30 days, wherein the first 7 days are the peak period of trapping, so the average daily number of Psoralea hibisci trapped by each trap in the first 7 days is detected and calculated, and the total number of Psoralea hibisci trapped by each trap for 30 days is detected and calculated, and the specificity is investigated at the same time, and the specificity is calculated as: Psoralea hibisci trapped / the number of all insects trapped*100%. The formula and trapping effect of the sex attractant composition are shown in Table 1, and the synergist is selected from any one of myrcene, α-pinene, β-pinene, cis-3-hexenol, and linalool.

[0061] Table 1. Formula of high-efficiency attractant for Phenacoccus hibiscus and its trapping effect

[0062]

[0063]

[0064] As shown in Table 1, when a single synergist is used for trapping, it is difficult to trap Pseudococcus hibiscus no matter which synergist is used; when sex pheromone is used for trapping alone, the trapping amount in 7 days reaches 17.2±0.49 heads / trap / day; when sex pheromone is used in combination with a single synergist for trapping, the average trapping amount in the first 7 days and the total trapping amount in 30 days show a significant decrease, regardless of which synergist is myrcene, α-pinene, β-pinene, cis-3-hexenol, or linalool; at the same time, by comparing Group 1 and Group 2, it can be seen that with the increase of the content of the synergist myrcene, there is still no synergistic effect when combined with sex pheromone. It can be seen that when the sex pheromone of Pseudococcus hibiscus is combined with a single synergist, there is no synergistic effect, and there may even be an inhibitory effect.

[0065] Example 2 Combination of Phenacoccus sangifera sex pheromone and two synergists

[0066] According to the method provided in Example 1, a sex attractant composition for Phenacoccus hibiscus was prepared to prepare a lure core and a trap, and the average daily capture amount of Phenacoccus hibiscus by each trap in the first 7 days and the total number of Phenacoccus hibiscus captured by each trap were detected and calculated, and the specificity was investigated at the same time. The specificity was calculated as follows: Phenacoccus hibiscus captured / the total number of insects captured*100%. The formula of the sex attractant composition and the trapping effect are shown in Table 2.

[0067] Table 2. Formula of high-efficiency attractant for Phenacoccus hibiscus and its trapping effect

[0068]

[0069]

[0070] As can be seen from Table 2, when only one synergist is used for trapping, no matter which synergist is used, it is difficult to trap the hibiscus mealybug; when sex pheromone is used alone for trapping, the 7-day trapping amount reaches 17.2±0.49 heads / trap / day; when two synergists are used but no sex pheromone (Group 2 and Group 4), it is also difficult to catch the hibiscus mealybug.

[0071] When sex pheromone is used in combination with two synergists, and the two synergists are α-pinene and β-pinene, the trapping effect on Phenacoccus hibiscus appears to be significantly enhanced, and the average trapping amount in the first 7 days and the total trapping amount in 30 days show a significant increase, especially when the content of sex pheromone is 200 parts, α-pinene 40 parts, and β-pinene 40 parts (Group 3), the synergistic effect is more significant. However, when sex pheromone is combined with myrcene and cis-3-hexenol (Group 5), or when sex pheromone is combined with myrcene and linalool (Group 6), or when sex pheromone is combined with cis-3-hexenol and linalool (Group 6), there is no synergistic effect, and even an inhibitory effect may occur.

[0072] It can be seen that when sex pheromone is used in combination with two synergists, a synergistic effect can only be achieved when the two synergists are α-pinene and β-pinene, and the degree of the synergistic effect is greatly affected by the mass fractions of sex pheromone, α-pinene and β-pinene. When the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, α-pinene and β-pinene is (5-10):(1-21):1, there is obvious synergism, especially when the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, α-pinene and β-pinene is 5:1:1, that is, 200 parts of sex pheromone, 40 parts of α-pinene and 40 parts of β-pinene, the synergistic effect is more obvious.

[0073] Example 3 Combination of Phenacoccus sibiricus Sex Pheromone and Three Synergists

[0074] According to the method provided in Example 1, a sex attractant composition for Phenacoccus hibiscus was prepared to prepare a lure core and a trap, and the average daily capture amount of Phenacoccus hibiscus by each trap in the first 7 days and the total number of Phenacoccus hibiscus captured by each trap were detected and calculated, and the specificity was investigated at the same time. The specificity was calculated as follows: Phenacoccus hibiscus captured / the number of all insects captured*100%. The formula of the sex attractant composition and the trapping effect are shown in Table 3.

[0075] Table 3. Formula of high-efficiency attractant for Phenacoccus hibiscus and its trapping effect

[0076]

[0077] As can be seen from Table 3, when sex pheromones were used alone for trapping, the capture amount in 7 days reached 17.2±0.49 heads / trap / day; when the three synergists were used alone for trapping without sex pheromones (Group 2 and Group 4), it was difficult to trap the hibiscus mealybug; and when sex pheromones were used in combination with three specific synergists (Group 1 and Group 3), the three specific synergists were myrcene, α-pinene and β-pinene, which showed a significant synergistic effect on the trapping effect of the hibiscus mealybug. The average capture amount in the first 7 days and the total capture amount in 30 days showed a significant increase, especially in Group 1, where the synergistic effect was very significant.

[0078] However, when sex pheromones were combined with the other three synergists (Group 5), there was no synergistic effect and even an inhibitory effect.

[0079] In addition, compared with the combination of sex pheromone and two synergists (Group 6 and Group 7), the synergistic effect was more obvious when sex pheromone was combined with three synergists (Group 1), and the effect of attracting hibiscus mealybugs was also better.

[0080] It can be seen that only when sex pheromones are used in combination with three specific synergists, and the three specific synergists are myrcene, α-pinene and β-pinene, can the synergistic effect be achieved, and the degree of synergistic effect is also affected by the ratio of sex pheromones, myrcene, α-pinene and β-pinene. When ((R)-isopentylenic acid-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester, myrcene, α-pinene and β-pinene are used in combination, the synergistic effect can be achieved. When the weight ratio of myrcene and β-pinene is 20:(2-4):1:(1-2), there is obvious synergism, especially when the weight ratio of (R)-isoprene acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, myrcene, α-pinene and β-pinene is 20:4:1:1, that is, 200 parts of sex pheromone, 40 parts of myrcene, 10 parts of α-pinene and 10 parts of β-pinene, the synergistic effect is more obvious.

[0081] Example 4 Combination of Phenacoccus sibiricus Sex Pheromone and Multiple Synergists

[0082] According to the method provided in Example 1, a sex attractant composition for Phenacoccus hibiscus was prepared to prepare a lure core and a trap, and the average daily capture amount of Phenacoccus hibiscus by each trap in the first 7 days and the total number of Phenacoccus hibiscus captured by each trap were detected and calculated, and the specificity was investigated at the same time. The specificity was calculated as follows: Phenacoccus hibiscus captured / the number of all insects captured*100%. The formula of the sex attractant composition and the trapping effect are shown in Table 4.

[0083] Table 4. Formula of high-efficiency attractant for Phenacoccus hibiscus and its trapping effect

[0084]

[0085]

[0086] The ratio of sex pheromones to synergists has a great influence on the control of hibiscus mealybugs. A slight adjustment in the ratio may affect the control effect. The ratio of various ingredients must be strictly controlled to obtain a very good synergistic effect.

[0087] As can be seen from Table 4, when sex pheromones were used alone for trapping, the capture amount in 7 days reached 17.2±0.49 heads / trap / day; when one or more synergists were used alone for trapping without sex pheromones (Groups 2, 4, 12-16), it was difficult to trap the hibiscus mealybug; and when sex pheromones were used in combination with a variety of specific synergists (Groups 1, 3, 5, 6, and 7), the trapping effect on the hibiscus mealybug was significantly synergistic, with a significant increase in the average capture amount in the first 7 days and the total capture amount in 30 days, especially in Group 3, where the synergistic effect was most significant.

[0088] However, when sex pheromones were combined with four synergists, namely α-pinene, β-pinene, cis-3-hexenol and linalool (Group 8), there was no synergistic effect and even an inhibitory effect was observed.

[0089] In addition, compared with the combination of sex pheromones with two synergists (Group 10) or three synergists (Group 9), the synergistic effect was more obvious when sex pheromones were combined with four synergists (Group 1) or five synergists (Group 3), and the effect of attracting hibiscus mealybugs was also better.

[0090] It can be seen that only when sex pheromones are used in combination with synergists of specific types and specific proportions can a synergistic effect be achieved, and the degree of the synergistic effect is affected to a certain extent by the number of synergistic agent types and the proportion relationship. The more synergistic agent types there are, the stronger the synergistic effect will be.

[0091] When the synergistic agent combination is four combinations and used together with sex pheromone, when the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is (20-40):(2-12):2:1:1, there is obvious synergism, especially when the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is 40:12:2:1:1, that is, 200 parts of sex pheromone, 60 parts of myrcene, 10 parts of α-pinene, 5 parts of β-pinene and 5 parts of cis-3-hexenol (Group 1), the synergistic effect is more obvious.

[0092] When the synergistic agent combination is five kinds of combinations and is used together with sex pheromones, as shown in Group 3 and Group 5, significant synergy can be obtained. Therefore, when the synergistic agent combination is five kinds of combinations, by mass fraction, 100 parts of R)-isopentynoic acid-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester, 1-99 parts of myrcene, 10-40 parts of α-pinene, 5-90 parts of β-pinene, 1-95 parts of cis-3-hexenol and 1-9 parts of linalool will all have significant synergy.

[0093] Among them, when the weight ratio of (R)-isopentenyl-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester, myrcene, α-pinene, β-pinene, cis-3-hexenol and linalool is 40:8:(2-3):(4-6):(2-9):1, there is an obvious synergistic effect, especially when the weight ratio of (R)-isopentenyl-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is 40:8:3:6:9:1, that is, 200 parts of sex pheromone, 40 parts of myrcene, 15 parts of α-pinene, 30 parts of β-pinene, 45 parts of cis-3-hexenol and 5 parts of linalool (Group 3), the synergistic effect is most significant, and the effect of attracting Hibiscus mealybug reaches the highest level.

[0094] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined by the claims.

Claims

1. A sex attractant composition for Phenacoccus hibiscus, characterized in that: The invention comprises sex pheromone and synergist, wherein the sex pheromone is (R)-isopentylenic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, the synergist is α-pinene and β-pinene, the weight ratio of (R)-isopentylenic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, α-pinene and β-pinene is (5-10):(1-21):1; the weight ratio of the sex pheromone to the synergist is 1:0.01-2.

0.

2. A sex attractant composition for Phenacoccus hibiscus, characterized in that: The invention comprises sex pheromone and synergist, wherein the sex pheromone is (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, the synergist is myrcene, α-pinene and β-pinene, the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, myrcene, α-pinene and β-pinene is 20:(2-4):1:(1-2); the weight ratio of the sex pheromone to the synergist is 1:0.01-2.

0.

3. A sex attractant composition for mealybugs, characterized in that: The invention comprises sex pheromone and synergist, wherein the sex pheromone is (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidene cyclobutyl) methyl ester, the synergist is myrcene, α-pinene, β-pinene and cis-3-hexenol, the weight ratio of (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylidene cyclobutyl) methyl ester, myrcene, α-pinene, β-pinene and cis-3-hexenol is (20-40):(2-12):2:1:1; the weight ratio of the sex pheromone to the synergist is 1:0.01-2.

0.

4. A sex attractant composition for Phenacoccus hibiscus, characterized in that: The invention comprises a sex pheromone and a synergist, wherein the sex pheromone is (R)-isopentylenic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, the synergist is myrcene, α-pinene, β-pinene and linalool, the weight ratio of (R)-isopentylenic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl) methyl ester, myrcene, α-pinene, β-pinene and linalool is 20:2:1:2:1; the weight ratio of the sex pheromone to the synergist is 1:0.01-2.

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5. A method for controlling or monitoring Phenacoccus hibiscus, characterized in that: The following steps are involved: 1) preparing an attractant core containing the composition according to any one of claims 1 to 4; 2) Place the bait core in the trap and place it in the place where the hibiscus mealybug needs to be controlled or monitored.

6. Use of the composition according to any one of claims 1 to 4 for preparing a preparation or bait for controlling or monitoring Phenacoccus hibiscus.

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