A mine heat-stable microbial dust suppressant and method of use thereof

Abstract

The present application belongs to the technical field of dust control in underground mines and open-pit mines, and particularly relates to a mine-used heat-stable microbial dust suppressant and a use method thereof, which is compounded by crude glycerol, precipitated ionic liquid and BM mixed solution in a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2, respectively. The BM mixed solution is a mixed solution of industrial strains and coal sample extracted strains, which is mixed with crude glycerol and precipitated ionic liquid in a certain proportion after being cultured for 24-48 hours under the optimal mold-producing condition to obtain the heat-stable microbial dust suppressant. The heat-stable microbial dust suppressant is uniformly sprayed to the dust on the open-pit slope, and the spraying amount is 6-10 L / m 3 per time, and the spraying is performed for 3-6 times. The results show that the crude glycerol extracted from waste biodiesel is more environmentally friendly than pure glycerol, and has a significant effect of improving the heat stability of urease and strengthening the dust suppression effect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of dust control in underground mines and open-pit mines, and particularly relates to a mine-used thermally stable microbial dust suppressant and a use method thereof. BACKGROUND

[0002] The "three-dusts" (blasting pile, coal pile, and dump) in open-pit coal mines and the dust in the roadway of underground mines will be affected by induced wind flow and release fine dust particles into the space environment, which will cause extremely serious harm to the surrounding environment and operating personnel. In order to effectively suppress the diffusion of mine dust, the traditional coal mine yard often adopts the way of dust suppression by windproof net and water spraying, but the actual dust suppression effect will be affected by environmental factors, resulting in unsatisfactory actual dust control effect.

[0003] For coal dust control in mines, a microbial dust suppression method relying on the decomposition of urea by mold to generate calcium carbonate precipitation to solidify dust (microbial induced calcite precipitation, MICP) has entered the field of vision. It not only can suppress the diffusion of dust into the atmosphere, but also can achieve the solidification of coal dust. Compared with traditional organic sand stabilizers, it has the characteristics of strong anti-aging, green environmental protection, and convenient construction. Although the patent with publication number CN103820123A discloses "a microbial sand stabilizing dust suppressant and a preparation method thereof", the patent with publication number CN110305629A discloses "a mine-used surfactant-microbial compound dust suppressant and a use method thereof", and the patent with publication number CN115029107A discloses "a microbial dust suppressant based on coal storage and transportation and a use method thereof", etc., but these microbial dust suppressants sprayed on the surface of coal dust have insufficient performance in terms of thermal stability, dust suppressant permeability, coal dust wettability, and wind erosion resistance. In particular, the activity of urease generated by the microbial dust suppressant is greatly affected by the environmental temperature, and the storage time of the microorganism is short. The microbial dust suppressant must be used within 3-5 days after preparation, otherwise the activity of urease will be greatly reduced, and the dust suppression effect will be reduced. In addition, the microbial dust suppressant can generate calcium carbonate between the pores of the dust particles, which has a solidification effect on the dust, but due to the instability of the dust suppressant, less calcium carbonate is generated, and the solidification effect on the dust is limited. The present application extracts crude glycerol from waste biodiesel based on the technology of microbial induced calcium carbonate precipitation, which not only meets the current pursuit of a green and low-carbon lifestyle, but also effectively increases the storage time of the microorganism, so that the storage time of the microbial dust suppressant can be increased to 30 days, and the thermal stability of urease is improved, thereby accelerating the cementation of coal particles and quickly forming a precipitate, which can achieve good dust suppression effect. SUMMARY

[0004] The application aims to provide a mine thermal stability microbial dust suppressant and a use method thereof, and by adding crude glycerol into a BM mixed solution, the thermal stability of the strain can be obviously improved, the high-activity storage time of the microbial solution is prolonged, the coal dust solidification and cementation efficiency and strength are improved, and the current problems such as slow reaction and low yield in the microbial carbonization process are effectively solved. In addition, the application of calcium magnesium acetate can reduce the freezing point of the microbial dust suppressant to below 0 DEG C, and the microbial dust suppressant can be applied to dust prevention and control work in a low-temperature environment. In addition, the method is green, environment-friendly, economical and practical, the carbonization deposit has high firmness coefficient, good anti-evaporation property and strong anti-wind erosion performance, and can effectively solve the problems of heap dust in open-pit coal mines and underground mines.

[0005] To achieve the above-mentioned purposes, the technical scheme adopted by the application is:

[0006] A mine thermal stability microbial dust suppressant, the dust suppressant is prepared by adding a mixed solution of crude glycerol, precipitated ionic liquid and BM mixed solution in a proportion of 0.8-1.2:1.8-2.2:1.8-2.2 by volume ratio into a culture medium, the crude glycerol is extracted from waste biodiesel; the precipitated ionic liquid is composed of a mixed solution of 0.8-1.2 mol / L urea and 1.3-1.7 mol / L calcium magnesium acetate, and is used to provide an alkaline environment and calcium ions for microbial growth; the BM mixed solution is prepared by mixing industrial strains and coal sample extraction strains, and the mass ratio between the two is 6:4, and the BM mixed solution concentration OD 600 =1.2.

[0007] Further, the crude glycerol is obtained by clarifying the waste biodiesel liquid, concentrating the glycerol to 40%, clarifying again, desalting and decolorizing, and finally distilling to obtain glycerol containing 85%, 2% NaOH, 5% water and 8% impurities, the impurities include 20% basic catalyst (KOH residue after reaction), 50% methanol, 20% ethanol, 10% incomplete reaction product glycerol monostearate glycerol distearate, 10% incomplete reaction product glycerol monostearate, glycerol distearate does not hinder the growth of microorganisms; other impurities have a certain promoting effect on the growth of microorganisms, and the basic catalyst contained therein can provide an alkaline environment for the growth of microorganisms; the alcohol substances such as methanol and ethanol can improve the decomposition amount of urea by the bacterial solution.

[0008] Further, the mass ratio of the crude glycerol and the culture medium solution is 1:3.5, and the culture medium solution is prepared by mixing 1.25 g of soybean peptone, 3.75 g of casein peptone, 1.25 g of NaCl, 5 g of urea and 250 ml of pure water, and is used to culture the BM mixed solution.

[0009] Further, the calcium magnesium acetate is obtained by mixing calcium acetate and magnesium acetate according to a mass ratio of 1:1, in addition, as a snow-melting agent, the calcium magnesium acetate can reduce the freezing point of water to below 0 DEG C after being dissolved in water, so that the dust prevention and control needs in a low-temperature environment are met.

[0010] Further, the industrial strain component is Bacillus pasteurii freeze-dried powder and Bacillus mucilaginosus, lysine bacillus, and the coal sample separated strain is one or more of bacillus, staphylococcus and aspergillus. The BM mixed strain is activated in a proteose peptone culture medium for 24-48 hours to obtain a bacterial liquid, and the concentration of the bacterial liquid is 7*10 7 ~14*10 7 The urease activity in the bacterial liquid is at a peak value.

[0011] A use method of the mine heat-stable microbial dust suppressant comprises the following steps: crude glycerol, BM mixed bacterial liquid and precipitated ionic liquid are configured according to a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2, and after being uniformly mixed, 6-10 L / m 3 The heat-stable microbial dust suppressant is sprayed 3-6 times, and after 7 days, the wind erosion resistance index, the penetration speed, the calcium carbonate generation amount and the firmness coefficient of the calcified solidification under natural conditions are respectively increased by 73%, 80%, 30%, 9% and 67%.

[0012] The solidification dust suppression mechanism of the heat-stable microbial dust suppressant is as follows: the BM mixed bacterial liquid is adsorbed on the surface of coal particles, the urease secreted by the BM mixed bacterial liquid can accelerate its own metabolism by using the nitrogen source of urea, and then a large amount of urease degradation products are generated, so that a large amount of ammonia gas (NH3) and carbamic acid (NH2COOH) are generated in water, the carbamic acid is further decomposed into NH4 + and CO3 2- , CO3 2- is combined with soluble calcium salt to form calcium carbonate crystals, and with the continuous increase of the calcium carbonate crystals, the coal particles are wrapped and a bridge is formed in the coal particles, so that the coal particles are cemented and the pores are filled.

[0013] The application has the following advantages:

[0014] 1. In the application, the calcium source of the mineralized substrate is positively charged, the surface of the BM mixed strain is negatively charged, the heat stability of the urease produced by the bacteria can be increased by adding the crude glycerol extracted from waste biodiesel, the storage time of the microorganism is prolonged, and the dust suppression time efficiency is further improved; in addition, as a snow-melting agent, the calcium magnesium acetate can reduce the freezing point of water to below 0 DEG C after being dissolved in water, so that the dust prevention and control needs in a low-temperature environment are met;

[0015] 2. The addition of crude glycerol to the heat-stable microbial environmentally friendly dust suppressant improves the heat stability of the BM mixed bacterial strain, reduces the loss of bacterial solution, improves the enzyme activity time in the bacterial solution, and prolongs the storage time of the carbonate mineralization bacteria;

[0016] 3. The crude glycerol is extracted from waste biodiesel, which can improve the heat stability of the bacterial solution and is more green and environmentally friendly without pollution;

[0017] 4. The heat-stable microbial environmentally friendly dust suppressant provided by the application is more simple and convenient to use, and can quickly achieve the dust suppression purpose;

[0018] 5. The bacterial mineralization is used for slope coal dust consolidation without secondary pollution, has the advantages of low price, simple operation and good dust suppression effect, has good prospects in open-pit mine dust control, and can be widely promoted;

[0019] 6. Compared with the chemical dust suppressant, the heat-stable microbial environmentally friendly dust suppressant has the characteristics of green environmental protection, convenient construction and strong permeability; compared with other microbial dust suppressants, the bacterial solution activity retention time is longer, the carbonate mineralization bacteria can be stored for 30 days, the substrate decomposition amount can reach 95% of the initial bacterial solution, and the test results of the coal dust sample consolidated by the heat-stable microbial dust suppressant show that the heat stability, wind erosion resistance index, calcium carbonate generation amount and firmness coefficient are improved by 73%, 80%, 30%, 9% and 67%. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 The influence of crude glycerol before and after addition on the heat stability of urease of the BM bacterial strain mixed solution at a temperature of 15-40 DEG C;

[0021] Figure 2 The influence of crude glycerol before and after addition on the heat stability of urease of the BM bacterial strain mixed solution at a temperature of 15-40 DEG C;

[0022] Figure 3 The influence of the heat-stable microbial dust suppressant before and after addition of crude glycerol on the wind erosion resistance intensity, penetration speed, calcium carbonate generation amount and firmness coefficient of coal dust;

[0023] Figure 4 The scanning electron microscope images of coal dust before and after cementation of the heat-stable microbial dust suppressant (a: before cementation; b: after cementation). DETAILED DESCRIPTION

[0024] Example 1

[0025] The application discloses a mine-used heat-stable microbial dust suppressant, which is prepared by adding a mixed solution of crude glycerol, precipitated ionic liquid and BM mixed solution into a culture medium at a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2, wherein the crude glycerol is extracted from waste biodiesel; the precipitated ionic liquid is composed of a mixed solution of 0.8-1.2 mol / L urea and 1.3-1.7 mol / L calcium-magnesium acetate, and is used for providing an alkaline environment and calcium ions for microbial growth; the BM mixed solution is prepared by mixing industrial strains and coal sample extraction strains, and the mass ratio of the industrial strains to the coal sample extraction strains is 6:4; the BM mixed strain solution has a concentration OD 600 =1.2; the crude glycerol is obtained by clarifying treatment of waste biodiesel liquid, concentrating the waste biodiesel liquid to 40% glycerol, performing second clarifying treatment, desalting and decoloring, and finally distilling to obtain glycerol containing 85%, NaOH containing 2%, water containing 5% and impurities containing 8%; the impurities include 20% alkaline catalyst (residual KOH in the reaction), 50% methanol, 20% ethanol and 10% incomplete reactants glycerol monostearate and glycerol distearate, which do not hinder the growth of microorganisms; other impurities have certain promotion effect on the growth of microorganisms, and the alkaline catalyst contained in the other impurities can provide an alkaline environment for the growth of microorganisms; the alcohol substances such as methanol and ethanol can improve the decomposition amount of urea by the strain solution; the mass ratio of the crude glycerol to the culture medium solution is 1:3.5; the culture medium solution is prepared by mixing 1.25 g of soybean peptone, 3.75 g of casein peptone, 1.25 g of NaCl, 5 g of urea and 250 ml of pure water, and is used for culturing the BM mixed solution.

[0026] The calcium-magnesium acetate is obtained by mixing calcium acetate and magnesium acetate at a mass ratio of 1:1; in addition, the calcium-magnesium acetate can reduce the freezing point of water to below 0 DEG C after being dissolved in water, so as to meet the dust control requirement in a low-temperature environment; the industrial strain components are freeze-dried powder of bacillus pasteurii and bacillus mucilaginosus, bacillus licheniformis and lysine bacillus; the coal sample separation strain is one or more of bacillus, staphylococcus and aspergillus; the BM mixed strain solution is prepared by activating the BM mixed strain solution in a peptone medium for 24-48 hours, and the strain solution has a concentration of 7*10 7 ~14*10 7 cell / mL, and the urease activity in the strain solution is a peak value.

[0027] The application further discloses a use method of the mine-used heat-stable microbial dust suppressant, which comprises the following steps: configuring crude glycerol, BM mixed strain solution and precipitated ionic liquid according to a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2, uniformly mixing the three, and then adding 6-10 L / m 3Spray 3~6 times, spray hot stable microbial environment-friendly dust suppressant on coal dust for 7d, and then measure the wind erosion resistance index, penetration rate, calcium carbonate generation amount, and firmness coefficient of the calcified consolidated substance under natural conditions, which are increased by 73%, 80%, 30%, 9%, and 67% respectively

[0028] Application Comparative Example 1

[0029] A mixed solution with a concentration of 0.8mol / L urea and 1.3mol / L calcium magnesium acetate, BM bacterial liquid with OD600=1.2, and a solution with a concentration of 8g / L crude glycerol are prepared, and then 50g of coal powder sample is weighed, the coal powder sample is sieved through a 160-mesh sieve, and then the three prepared solutions are mixed and placed in a spraying device (denoted as treatment 1), and the volume ratio is 0.8:1.8:1.8.

[0030] As a control, the same concentration of urea, calcium magnesium acetate mixed solution and bacterial liquid are mixed uniformly and sprayed into the coal powder (denoted as treatment 2).

[0031] Finally, the coal powder sprayed with the biological dust suppressant is placed at room temperature for natural air drying, and after 3d, the biological dust suppressant is sprayed again in the above two ways, and the similarly treated coal powder is placed at room temperature for natural air drying for 3d.

[0032] After the treatment is completed, the treated coal powder is subjected to wind erosion resistance experiment, penetration rate test experiment, calcium carbonate generation amount test experiment and firmness coefficient test experiment, wherein the wind speed is 10m / s, and the results are shown in Table 1.

[0033] Table 1 Performance test results of coal powder under different treatment modes

[0034]

[0035] Application Comparative Example 2

[0036] A mixed solution with a concentration of 1.0mol / L urea and 1.5mol / L calcium magnesium acetate, BM bacterial liquid with OD600=1.2, and a solution with a concentration of 10g / L crude glycerol are prepared, and then 50g of coal powder sample is sieved through a 180-mesh sieve, and then the three prepared solutions are mixed and placed in a spraying device (denoted as treatment 1), and the volume ratio is 1:2:2.

[0037] As a control, the same concentration of urea, calcium magnesium acetate mixed solution and bacterial liquid are mixed uniformly and sprayed into the coal powder (denoted as treatment 2).

[0038] Finally, the coal powder sprayed with the biological dust suppressant is placed at room temperature for natural air drying, and after 3d, the biological dust suppressant is sprayed again in the above two ways, and the similarly treated coal powder is placed at room temperature for natural air drying for 3d.

[0039] After the treatment, the treated coal powder is subjected to wind erosion resistance experiment, permeation speed test experiment, calcium carbonate generation amount test experiment and firmness coefficient test experiment, wherein the wind speed is 12 m / s, and the results are shown in Table 2.

[0040] Table 2: Performance test results of coal powder under different treatment modes

[0041]

[0042] Application Comparative Example 3

[0043] A mixed solution with a concentration of 1.2 mol / L urea and 1.7 mol / L calcium magnesium acetate, BM bacterial liquid with OD600 = 1.2 and a solution with a concentration of 12 g / L crude glycerol are prepared, 50 g of coal powder sample is weighed and sieved through a 200 mesh sieve, and then the three prepared solutions are mixed and placed in a spraying device (referred to as treatment 1), and the volume ratio is 1.2:2.2:2.2.

[0044] As a control, the same concentration of urea, calcium magnesium acetate mixed solution and bacterial liquid are mixed uniformly and sprayed into the coal powder (referred to as treatment 2).

[0045] Finally, the coal powder sprayed with the biological dust suppressant is placed at room temperature for natural air drying, and after 3 days, the biological dust suppressant is sprayed again according to the above two-week method, and the similarly treated coal powder is placed at room temperature for natural air drying for 3 days.

[0046] After the treatment, the treated coal powder is subjected to wind erosion resistance experiment, permeation speed test experiment, calcium carbonate generation amount test experiment and firmness coefficient test experiment, wherein the wind speed is 14 m / s, and the results are shown in Table 3.

[0047] Table 3: Performance test results of coal powder under different treatment modes

[0048]

[0049] As can be seen from Tables 1, 2 and 3, the addition of crude glycerol has the most significant effect on the wind resistance index and the permeation speed, and the influence degree of both is more than 50%. Moreover, the reaction time of calcium carbonate generation is accelerated, and the yield is also significantly improved, with an average yield increase of 27%, and the calcium carbonate generation amount tends to be a fixed value at the 5th hour, while the product without crude glycerol needs 7-8 hours to react to stop. In addition, the product firmness coefficient is also improved, and from the SEM electron microscope image, it can be seen that the product crystallization state is more dense after the addition of crude glycerol. The penetration speed of the dust suppressant into the coal powder is also improved by about 30%, and the overall dust suppression effect is good, and the product generation time and speed are also relatively obviously improved.

[0050] Verification Test

[0051] In order to determine the influence of the crude glycerol prepared from waste biodiesel on the thermal stability of urease of the BM strain mixture, six experimental groups are set, after being placed for 7 days, the substrate decomposition amount of the initial bacterial liquid under the conditions of -5, 0, 4, 15, 30 and 40 DEG C after the action of crude glycerol is respectively measured, and the substrate decomposition amount under the condition of not adding crude glycerol is compared, so as to determine whether the crude glycerol can improve the thermal stability of urease of the BM strain mixture.

[0052] The coal powder is injected in the following manner: A: the mixed solution of mineralized substrate and BM bacterial liquid is injected into the coal powder; B: the mixed solution of crude glycerol, mineralized substrate and BM bacterial liquid is injected into the coal powder. The mixed solution is injected into the coal powder, the mineralized substrate decomposition amount before and after the injection of crude glycerol is measured by using the colorimetric method, and the mineralized substrate decomposition amount before and after the injection is taken as the measurement index of the thermal stability of urease. The influence of crude glycerol on the thermal stability of urease of the BM strain mixture is shown in the following table. Figure 1

[0053] From the table, it can be seen that under the condition of the initial BM bacterial liquid amount being 8.2 g, after the bacterial liquid is stored for 10 days at three kinds of storage temperatures after the addition of crude glycerol, the BM bacterial liquid decomposition amount respectively reaches 73%, 63% and 44% of the initial content. This indicates that the addition of crude glycerol can improve the enzyme activity, but under the condition of the temperature being higher than 20 DEG C, the increase range of the initial BM bacterial liquid decomposition amount after the addition of crude glycerol is not obvious, which indicates that the effect of crude glycerol is limited under the condition of higher temperature. Considering the actual use requirement, under the condition of normal temperature (10-20 DEG C), the present application selects the crude glycerol with a volume fraction of 20% as the reagent for improving the enzyme activity. Figure 1 From the table, it can be seen that under the condition of the initial BM bacterial liquid amount being 8.2 g and the temperature environment being (-5-4 DEG C), after the addition of the crude glycerol reagent and being stored for 20 days, the difference of the BM bacterial liquid decomposition amount is not large, the maximum decomposition amount of the BM bacterial liquid is 7.94 g under the condition of the crude glycerol reagent with a volume fraction of 10% at 4 DEG C, which reaches 97% of the initial BM bacterial liquid amount, which indicates that the BM bacterial liquid is in a dormant state under the condition of low temperature environment, the life activity stops, the enzyme activity is stable, and the low temperature environment can be used as the storage temperature for long-term storage of the bacterial liquid.

[0054] Figure 2 The addition of the crude glycerol extract of waste biodiesel can protect the stable enzyme activity, the hydroxyl group of the crude glycerol and the amide group of the enzyme interact with each other in the form of hydrogen bond, so as to improve the thermal stability of the enzyme. The crude glycerol molecule is small, can be combined in the gap of the peptide chain of the enzyme molecule, enhances the intramolecular hydrophobicity, and reduces the interaction of the enzyme molecules. In addition, the crude glycerol can prevent part of the substances leading to the inactivation of the enzyme from approaching the active center, and further improves the thermal stability of the enzyme.

[0055] The addition of the crude glycerol extract of waste biodiesel can protect the stable enzyme activity, the hydroxyl group of the crude glycerol and the amide group of the enzyme interact with each other in the form of hydrogen bond, so as to improve the thermal stability of the enzyme. The crude glycerol molecule is small, can be combined in the gap of the peptide chain of the enzyme molecule, enhances the intramolecular hydrophobicity, and reduces the interaction of the enzyme molecules. In addition, the crude glycerol can prevent part of the substances leading to the inactivation of the enzyme from approaching the active center, and further improves the thermal stability of the enzyme.

[0056] ​​In addition, to investigate the effects of different treatment methods on the wind erosion resistance, calcium carbonate formation, penetration rate, and robustness coefficient of pulverized coal, the following treatment methods were used: a) spraying a mixture of mineralized substrate and BM bacterial solution; b) spraying a mixture of crude glycerol, mineralized substrate, and BM bacterial solution. The volume of the sprayed solution was the same. After a certain treatment time, the pulverized coal was dried, and wind erosion resistance, calcium carbonate formation, penetration rate, and robustness coefficient were tested. The test results are attached. Figure 2 As shown.

[0057] From the appendix Figure 2 As can be seen, after adding crude glycerol, the wind erosion resistance, calcium carbonate formation, penetration rate, and robustness coefficient of the mineralized products increased by 80%, 30%, 9%, and 67%, respectively, indicating that the addition of crude glycerol can improve the characteristic structure of the mineralized substrate. This is actually because crude glycerol improves the thermal stability of the bacterial solution, thereby promoting a rapid reaction between the bacterial solution and coal powder, significantly accelerating the reaction time.

[0058] Appendix Figure 3 This is a scanning electron microscope (SEM) image after spraying crude glycerol, mineralized substrate, and BM bacterial solution. The image shows that the mineralized product produced by adding crude glycerol has a more compact structure between its fine crystals, generates more calcium carbonate, and can fill the voids in the coal powder particles, cementing them into a cohesive whole. This significantly improves the product's resistance to wind erosion and its robustness.

Claims

1. A mine dust suppressant of thermally stable microorganisms, characterized by: The dust suppressant is prepared by mixing crude glycerol, precipitated ionic liquid and BM mixed solution in a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2, and then adding the mixture to the culture medium, wherein the crude glycerol is extracted from waste biodiesel; the precipitated ionic liquid is composed of a mixed solution of 1.0 mol / L urea and 1.5 mol / L calcium magnesium acetate; the BM mixed solution is prepared by mixing industrial strains and coal sample extraction strains, and the mass ratio between the two is 6:4; the crude glycerol is obtained by clarifying the waste biodiesel liquid, concentrating the glycerol to 40%, clarifying again, desalting and decolorizing, and finally distilling to obtain glycerol containing 85%, NaOH 2%, water 5% and impurities 8%, wherein the impurities include 20% basic catalyst, 50% methanol, 20% ethanol and 10% incomplete reactants glycerol monostearate and glycerol distearate; the mass ratio of the crude glycerol and the culture medium solution is 1:3.5, and the culture medium solution is prepared by mixing 1.25 g of soybean peptone, 3.75 g of casein peptone, 1.25 g of NaCl, 5 g of urea and 250 ml of pure water; the industrial strain components are bacillus pasteurii freeze-dried powder, bacillus mucilaginosus and bacillus lysine, and the coal sample separation strain is one or more of bacillus, staphylococcus and aspergillus; after mixing the industrial strains and the coal sample separation strains, they are activated in the peptone medium for 24-48 h to obtain the bacterial solution, and the concentration of the bacterial solution is 7×10 7 ~14×10 7 cell / mL.

2. The thermally stable microbial dust suppressant for mining as claimed in claim 1, wherein: The calcium magnesium acetate is obtained by mixing calcium acetate and magnesium acetate in a mass ratio of 1:

1.

3. The method of using a thermophilic microbial dust suppressant for mining as claimed in any one of claims 1 to 2, wherein, comprising the steps of: The crude glycerol is mixed with the mixed bacteria liquid, the precipitated ionic liquid, and water in a volume ratio of 0.8-1.2:1.8-2.2:1.8-2.2:1.8-2.2, and after being mixed uniformly, 6-10 L / m 3 Spray 3-6 times at the dosage.

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