Application of hematoporphyrin monomethyl ether and growth regulator containing hematoporphyrin monomethyl ether

Abstract

The invention discloses an application of hematoporphyrin monomethyl ether and a growth regulator containing hematoporphyrin monomethyl ether, wherein the growth regulator comprises hematoporphyrin monomethyl ether, and the mass fraction of hematoporphyrin monomethyl ether in the growth regulator is 6-13%. The growth regulator in the invention can promote seed germination and inhibit seed germination at the same time, and it mainly inhibits or promotes seed germination by regulating the level of endogenous hormones in plants; in addition, the growth regulator can also enhance or weaken the photosynthesis of leaves by regulating the degradation and synthesis of chlorophyll in plants, thereby controlling the plant height and fresh weight of plant seedlings.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulators, and in particular to an application of hematoporphyrin monomethyl ether and a growth regulator containing the hematoporphyrin monomethyl ether. Background Art

[0002] At present, although the use of biological pesticides is advocated, their shortcomings such as long cycle, slow effect, and easy inactivation still make their market share inferior to chemical pesticides. As we know, chemical pesticides are highly toxic and have high residues, which have 3R problems. Therefore, constructing compounds with clear targets and environmental safety is a key point in the development of chemical pesticides.

[0003] APDT (anti-microbial photodynamic therapy) is based on the concept of photodynamic therapy (PDT). It uses the preferential aggregation characteristics of photosensitizers on bacteria. Under the excitation of low-dose visible light of appropriate wavelength, photosensitizers absorb energy and produce reactive oxygen species such as singlet oxygen and free radicals. Reactive oxygen kills target cells through oxidation without harming surrounding tissues and cells. Bertoloni et al. discovered in 1984 that photodynamic therapy (PDT) can kill bacteria. Photodynamic inactivation technology (PDI) is a new type of non-thermal sterilization technology. It is a sterilization technology that uses reactive oxygen species produced by light-activated photosensitizers to cause microbial death. Studies have shown that PDI can effectively kill bacteria such as Staphylococcus aureus, Escherichia coli and Salmonella, and has significant sterilization effects on fungi such as Aspergillus niger, Aspergillus flavus, Penicillium griseo-yellow and Candida albicans. It can also kill Duran virus, H5N8 avian influenza virus, etc. In addition, its biggest advantage is that it is not easy to make harmful microorganisms tolerant.

[0004] Hematoporyrin monomethyl ether (HMME) is a new type of photosensitizer independently developed in my country. Its chemical name is 3-(or 8-)(1-methoxyethyl)-8-(or -3-)(1-hydroxyethyl)-porphyrin IX or hematoporphyrin 3- or 8-monomethyl ether. It is an isomer mixture of two monomeric porphyrins that are positional isomers of each other. It has a stable chemical composition and has the advantages of short light avoidance time, high singlet oxygen production, long absorption wavelength, and low relative content of porphyrins that cause phototoxic reactions in normal tissues. Luan Xiaomin et al. found that HMME-mediated photodynamic antimicrobial therapy can effectively kill Porphyromonas gingivalis; Wu Qiuju et al. observed the clinical reactions of patients after taking the medicine and concluded that HMME-PDT treatment for port wine stains has the advantages of high efficiency, safety, and low incidence of adverse reactions. It has been clinically used in the treatment of port wine stains, tumors and other diseases; Jin Hua et al. observed the changes in the morphology of bacteria before and after the action of HMME by plate colony counting method and atomic force microscopy (AFM) and found that when the HMME concentration was 50 / mL and visible light was irradiated for 30 minutes, more than 90% of Staphylococcus aureus could be killed, specifically acting on the cell membrane on the surface of the bacteria.

[0005] However, there is currently no record of hematoporphyrin monomethyl ether being used as a plant growth regulator. Summary of the invention

[0006] In order to solve the problem of high residue of existing chemical pesticides, one of the purposes of the present invention is to provide an application of hematoporphyrin monomethyl ether in regulating plant growth.

[0007] The technical solution of the present invention to solve the above technical problems is as follows:

[0008] Application of hematoporphyrin monomethyl ether in regulating plant growth.

[0009] The beneficial effects of the present invention are as follows: the hematoporphyrin monomethyl ether in the present invention is sensitive to light, easily degraded, and avoids the problem of high residue, so it is environmentally safe; in addition, the hematoporphyrin monomethyl ether in the present invention has the function of regulating plant seed germination, plant seedling growth and seedling fresh weight.

[0010] On the basis of the above technical solution, the present invention can also be improved as follows:

[0011] Furthermore, the plant is a cereal, a leguminous plant or a solanaceous plant.

[0012] Furthermore, the cereal plant is wheat, the leguminous plant is soybean, and the nightshade plant is eggplant.

[0013] Further, the plant seeds are immersed in a solution of hematoporphyrin monomethyl ether at a concentration of 1 to 5×10 -5mg / L solution for 10 to 24 hours; wherein the concentration of hematoporphyrin monomethyl ether is measured as the concentration of hematoporphyrin monomethyl ether.

[0014] Furthermore, the plant seeds were immersed in a solution of hematoporphyrin monomethyl ether at a concentration of 1×10 -3 ~2×10 -4 mg / L solution; wherein the concentration of hematoporphyrin monomethyl ether is measured as the concentration of hematoporphyrin monomethyl ether.

[0015] The beneficial effect of adopting the above further technical solution is that the solution containing hematoporphyrin monomethyl ether in this concentration range can promote seed germination and thus improve the germination rate.

[0016] Furthermore, the concentration of hematoporphyrin monomethyl ether applied to the plants at the seedling stage is 1 to 5×10 -5 mg / L solution 3 to 5 times, and the application amount of the solution per plant each time is 0.2 to 0.5L; wherein the concentration of hematoporphyrin monomethyl ether is measured as hematoporphyrin monomethyl ether concentration.

[0017] Furthermore, hematoporphyrin monomethyl ether was applied to the plants at the seedling stage at a concentration of 1×10 -3 ~2×10 -4 mg / L solution; wherein the concentration of hematoporphyrin monomethyl ether is measured as hematoporphyrin monomethyl ether concentration.

[0018] The beneficial effect of adopting the above further technical solution is that the solution containing hematoporphyrin monomethyl ether in this concentration range can promote the elongation of plant seedlings and increase the fresh weight of seedlings.

[0019] The second object of the present invention is to provide a growth regulator containing the hematoporphyrin monomethyl ether in the first object.

[0020] Furthermore, the mass fraction of hematoporphyrin monomethyl ether in the growth regulator is 6-13%.

[0021] Furthermore, the mass fraction of hematoporphyrin monomethyl ether in the growth regulator is 10%.

[0022] The present invention has the following beneficial effects:

[0023] The growth regulator in the present invention can both promote seed germination and inhibit seed germination, and it mainly inhibits or promotes seed germination by regulating the level of endogenous hormones in plants; in addition, hematoporphyrin monomethyl ether regulates the degradation and synthesis of chlorophyll in plants, enhances or weakens the photosynthesis of leaves, thereby controlling the plant height and fresh weight of plant seedlings. DETAILED DESCRIPTION

[0024] The application of hematoporphyrin monomethyl ether and the growth regulator containing hematoporphyrin monomethyl ether in the present invention will be described below in conjunction with test examples.

[0025] However, the present invention can be exemplified in many different forms and should not be construed as limited to the specific experimental examples set forth herein. Rather, these experimental examples are provided so that the present invention will be thorough and complete and will fully convey the scope of the present invention to those skilled in the art.

[0026] An embodiment of the first aspect of the present invention provides a use of hematoporphyrin monomethyl ether in regulating plant growth.

[0027] In the present invention, hematoporphyrin monomethyl ether can both promote seed germination and inhibit seed germination, and it mainly inhibits or promotes seed germination by regulating the level of endogenous hormones in plants; in addition, hematoporphyrin monomethyl ether regulates the degradation and synthesis of chlorophyll in plants, enhances or weakens the photosynthesis of leaves, thereby controlling the plant height and fresh weight of plant seedlings.

[0028] In addition, in some embodiments, the plant seeds are soaked in a solution of hematoporphyrin monomethyl ether at a concentration of 1 to 5×10 -5 Preferably, the plant seeds are immersed in a solution with a concentration of 1×10 -3 ~2×10 -4 In this embodiment, the solution in this concentration range can promote seed germination, thereby increasing the germination rate. In addition, the concentration of hematoporphyrin monomethyl ether in this embodiment is measured in terms of hematoporphyrin monomethyl ether concentration.

[0029] In addition, in some embodiments, the concentration of hematoporphyrin monomethyl ether applied to the plant at the seedling stage is 1-5×10 - 5 mg / L solution 3 to 5 times, and the amount of solution applied to each plant each time is 0.2 to 0.5 L. Preferably, the concentration of hematoporphyrin monomethyl ether applied to plants at the seedling stage is 1×10 -3 ~2×10 -4 In this embodiment, the solution in this concentration range can promote the elongation of plant seedlings and increase the fresh weight of seedlings. In addition, the concentration of hematoporphyrin monomethyl ether in this embodiment is measured as hematoporphyrin monomethyl ether concentration.

[0030] In addition, the hematoporphyrin monomethyl ether in this embodiment is a product that can be purchased on the market, and can be the hematoporphyrin monomethyl ether sold by Shanghai Standard Standard Technology Service Co., Ltd.

[0031] In addition, in some embodiments, the plants are cereals, legumes and solanaceous plants; preferably, the cereal plants are wheat, the legumes are soybeans, and the solanaceous plants are eggplants. In addition, in practice, hematoporphyrin monomethyl ether can also act on plants of the same family or close family to wheat, soybeans and eggplants.

[0032] The embodiment of the second aspect of the present invention provides a growth regulator containing hematoporphyrin monomethyl ether, wherein the growth regulator includes hematoporphyrin monomethyl ether, and the mass fraction of hematoporphyrin monomethyl ether in the growth regulator is 6-13%; preferably, the mass fraction of hematoporphyrin monomethyl ether in the growth regulator is 10%.

[0033] In addition, in practice, the dosage form of the growth regulator in this embodiment is an aqueous solution, a suspension, a wettable powder or a granule.

[0034] In this embodiment, when the growth regulator is an aqueous solution, the growth regulator also includes a solvent with a mass fraction of 0.1%, a stabilizer with a mass fraction of 2%, an antifreeze with a mass fraction of 5%, and a preservative with a mass fraction of 0.1-0.5%; wherein the solvent is xylene, toluene or cyclohexanone, the stabilizer is triphenyl phosphite or epichlorohydrin, the antifreeze is ethylene glycol, propylene glycol, glycerin or urea, and the preservative is benzoic acid or sodium benzoate.

[0035] When the growth regulator is a suspending agent, the growth regulator also includes 3% of a dispersant by mass, 5-7% of a wetting agent, 2% of a thickener, 0.1-0.5% of a preservative, 0.1-0.5% of a defoaming agent and 4% of an antifreeze agent; wherein the dispersant is alkyl naphthalene sulfonate, polycarboxylate or lignin sulfonate, the wetting agent is alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate or naphthalene sulfonate, the thickener is xanthan gum, magnesium aluminum silicate or bentonite, the preservative is benzoic acid or sodium benzoate, the defoaming agent is an organosilicon, such as polydimethylsiloxane; and the antifreeze agent is glycerol, urea, ethylene glycol or propylene glycol.

[0036] When the growth regulator is a wettable powder, the growth regulator also includes 6% by mass of a dispersant, 3% of a wetting agent and 4-5% of a filler; wherein the dispersant is a polycarboxylate, a lignin sulfonate or an alkyl naphthalene sulfonate, the wetting agent is an alkyl sulfonate, an alkyl sulfate or a naphthalene sulfonate, and the filler is light calcium carbonate, talc, diatomaceous earth, kaolin or attapulgite.

[0037] When the growth regulator is a granule, the growth regulator also includes 4% of a dispersant, 5% of a wetting agent, 5% of a disintegrant, 0.3% of a binder and 28-37% of a filler in a mass fraction; wherein the dispersant is a polycarboxylate, a lignin sulfonate or an alkyl naphthalene sulfonate, the wetting agent is an alkyl sulfate, polyoxyethylene alcohol, an alkyl sulfonate or a naphthalene sulfonate, the disintegrant is citric acid, ammonium sulfate, glucose, urea or sodium bicarbonate, the binder is corn starch, microcrystalline cellulose or diatomaceous earth, and the filler is light calcium carbonate, talc, diatomaceous earth, kaolin or attapulgite.

[0038] In addition, in practice, the various adjuvants in the above-mentioned different dosage forms of growth regulators (adjuvants are any of emulsifiers, wetting agents, stabilizers, thickeners, preservatives, antifreeze agents, dispersants, fillers, disintegrants and binders) can be used as adjuvants in the growth regulators in this embodiment as long as they can improve the physical and chemical properties of the agent, enhance the efficacy, and facilitate transportation and storage during the processing or use of the growth regulator.

[0039] In addition, "%" in the present examples refers to mass percentage.

[0040] Example

[0041] Example 1: Study on the effect of hematoporphyrin monomethyl ether on seed germination

[0042] Take wheat (variety Chuanmai 601), soybean (variety Zhonghuang 57), and eggplant (variety Zirong 2) seeds.

[0043] Prepare the experimental solution: dilute 80% hematoporphyrin monomethyl ether to 1×10 with distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80. 3 , 5×10 3 , 5×10 4 , 5×10 5 , 1×10 6 , 5×10 6 , 1×10 7 , 2×10 7 The solution containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 in distilled water was used as the control group.

[0044] The soaking germination test was conducted on wheat, soybean and eggplant seeds. Specifically, 50 seeds were placed in each culture dish, each concentration was repeated 3 times, and the seeds were cultured at 25°C for 48 hours. The germination of wheat and soybean was observed. After the eggplant was cultured for 148 hours under the same conditions, the germination of the eggplant was observed, and the germination rate was calculated according to the following formula:

[0045] Germination rate = number of germinated seeds / total number of seeds × 100%.

[0046] Table 1. Germination rate of wheat, soybean and eggplant seeds under different concentrations of hematoporphyrin monomethyl ether

[0047]

[0048]

[0049] Note: DPS 6.0 data processing software was used for statistical analysis, and Duncan's new multiple range method was used for analysis of significance of differences; different lowercase letters after the data in the same column indicate significant differences (P<0.05).

[0050] As can be seen from Table 1, hematoporphyrin monomethyl ether has an effect on the germination of wheat, soybean and eggplant seeds, specifically:

[0051] For wheat, hematoporphyrin monomethyl ether at a concentration of 1-2×10 -2 mg / L, it inhibited seed germination. -3 ~5×10 -5 mg / L, the germination of wheat was close to that of the control group, and there was no significant difference in germination rate. The effect of the agent on the germination rate of soybean seeds was as follows: -2 ~5×10 -5 mg / L has no significant effect on seed germination. For eggplant seeds, the concentration is 1-2×10 -2 The germination rate of seeds soaked with the agent in the mg / L range was lower than that of the control. -3 ~5×10 -5 Treating seeds with low concentration of pesticide at 1000 mg / L had no effect on seed germination.

[0052] Example 2: Study on the effect of hematoporphyrin monomethyl ether on plant seedling growth

[0053] Healthy wheat seedlings, soybean seedlings, and eggplant seedlings with similar growth conditions were selected and transplanted into pots, with 5 plants per pot, 10 pots per group, and 4 replicates per group.

[0054] The same experimental solution as in Example 1 was prepared, with distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as a control group.

[0055] Each group of potted plants was sprayed, with a single spraying volume of 0.3 L per plant per day, a spraying cycle of 5 days, and a total of 5 sprayings. After spraying, the plants were placed in a 25°C environment for 5 days, and the plant heights of each plant before and after cultivation were recorded.

[0056] Table 2. Plant height of wheat, soybean and eggplant under different concentrations of hematoporphyrin monomethyl ether

[0057]

[0058]

[0059] Note: DPS 6.0 data processing software was used for statistical analysis, and Duncan's new multiple range method was used for analysis of significance of differences; different lowercase letters after the data in the same column indicate significant differences (P<0.05).

[0060] As can be seen from Table 2, hematoporphyrin monomethyl ether has a certain effect on the plant height of wheat, soybean and eggplant, specifically:

[0061] Effect of the agent on wheat: When the concentration of the agent is 1×10 -3 ~1×10 -4 mg / L, the elongation of wheat was significantly improved compared with the control group, while at 1-2×10 -2 mg / L concentration showed inhibition on wheat plant height; for soybean, the concentration of the agent was 1-2×10 -1 mg / L treatment, the soybean plants in the treatment group were significantly lower than those in the control group, while the concentration of 2×10 -4 ~5×10 -5 mg / L of the agent has a promoting effect on plant elongation; the plant height of all treatment groups has no significant difference with the control, but the concentration of the agent is 1×10 -3 The eggplant plant height in the treatment group was the largest.

[0062] Example 3: Study on the effect of hematoporphyrin monomethyl ether on fresh weight and dry weight of plants

[0063] Healthy wheat seedlings, soybean seedlings, and eggplant seedlings with similar growth conditions were selected and transplanted into pots, with 5 plants per pot, 10 pots per group, and 4 replicates per group.

[0064] The same experimental solution as in Example 1 was prepared, with distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as a control group.

[0065] The potted plants were sprayed in each group. Each spraying cycle was 5 days. Each plant was sprayed once a day, and the spraying volume was 0.3 L each time. The fresh weight of the plants was counted 5 days after the fifth spraying. The plants were then placed in an oven at 60°C and dried to constant weight. The dry weight of the plants was recorded.

[0066] Table 3. Fresh weight and dry weight of wheat, soybean and eggplant under different concentrations of hematoporphyrin monomethyl ether

[0067]

[0068] Note: DPS 6.0 data processing software was used for statistical analysis, and Duncan's new multiple range method was used for analysis of significance of differences; different lowercase letters after the data in the same column indicate significant differences (P<0.05).

[0069] As can be seen from Table 3, hematoporphyrin monomethyl ether has a certain impact on the dry weight and fresh weight of wheat, soybean and eggplant. Specifically:

[0070] For wheat, the concentration of the agent is 1-2×10 -1 mg / L, the fresh weight of crops in the treatment group was lower than that in the control group, and the agent inhibited the accumulation of crop fresh weight; the concentration of the agent was 2×10 -3 ~5×10-5 mg / L showed a promoting effect on the fresh weight of plant seedlings, and the treatment group showed a significant difference from the control group; in terms of wheat dry weight, except for 1-2×10 -1 The dry weight of wheat in the other treatment groups except the treatment group with the concentration of 2×10 -4 The dry weight of the soybean treated group was the largest when the concentration of the agent was 1×10 -3 ~5×10 -5 The fresh weight and dry weight of the treatment group were significantly higher than those of the control group, and the fresh weight and dry weight were both within 2×10 -4 mg / L concentration of the drug reached the maximum after treatment. For eggplant, the drug concentration was 2×10 -2 ~5×10 -5 The fresh weight of crops in the treatment group was higher than that in the control group. -3 ~2×10 - 4 The dry weight of crops in the treatment group with 2×10 -4 mg / L treatment of crops can better promote the accumulation of crop dry weight and fresh weight.

[0071] The above description is only a preferred test example of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Application of hematoporphyrin monomethyl ether in regulating plant growth.

2. The use according to claim 1, characterized in that: The plants are cereals, leguminous plants and solanaceous plants.

3. The use according to claim 2, characterized in that: The cereal plant is wheat, the leguminous plant is soybean, and the solanaceous plant is eggplant.

4. The use according to claim 3, characterized in that: Plant seeds were soaked in hematoporphyrin monomethyl ether at a concentration of 1-5×10 -5 mg / L solution for 10~24h.

5. The use according to claim 4, characterized in that: Plant seeds were soaked in hematoporphyrin monomethyl ether at a concentration of 1×10 -3 ~2×10 -4 mg / L solution.

6. The use according to claim 3, characterized in that: When the concentration of hematoporphyrin monomethyl ether was 1~5×10 -5 Apply 0.2~0.5L of solution per plant 3~5 times.

7. The use according to claim 6, characterized in that: When the concentration of hematoporphyrin monomethyl ether was 1×10 -3 ~2×10 -4 mg / L solution.

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