Antibacterial peptide and its antibacterial use

Abstract

The present application relates to the field of molecular biology, and particularly relates to an antibacterial peptide and application thereof.The antibacterial peptide is shown in the amino acid sequence in the sequence table SEQ ID No.1.The antibacterial peptide is used for preparing a bactericidal preparation.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of molecular biology, and particularly relates to an antibacterial peptide and application thereof. BACKGROUND

[0002] Antibacterial peptide is a kind of polypeptide with broad-spectrum antibacterial, antiviral and even antiparasitic activity. Antibacterial peptide can act on bacterial cell membrane, destroy the integrity of the cell membrane, thereby playing a role in killing bacteria. Unlike traditional antibiotic drugs, the unique bactericidal mechanism of antibacterial peptide will not make the target bacteria resistant, so antibacterial peptide is attracting more and more attention. Antibacterial peptides exist widely in microorganisms and animals and plants, and so far, more than 2600 antibacterial peptides have been found, and more than 70 antibacterial peptides have been identified in fish. Antibacterial peptides are an important part of the non-specific immune system and play an important role in resisting pathogenic infection. Vibrio harveyi is a gram-negative bacterium and is one of the main pathogens of aquatic animals, which can infect a variety of marine and freshwater animals, including aquaculture animals. Micrococcus luteus belongs to gram-positive bacteria and can infect hosts such as humans, causing bacteremia and the like. The research and application of antibacterial peptides against these pathogenic bacteria will help the prevention and control of related diseases. SUMMARY

[0003] The present application aims to provide an antibacterial peptide and antibacterial application thereof.

[0004] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0005] An antibacterial peptide, characterized in that the antibacterial peptide is shown in the amino acid sequence in the sequence table SEQ ID No. 1.

[0006] The sequence table SEQ ID No. 1 is as follows:

[0007] KKRRRGLSRFRMGRGY

[0008] An application of the antibacterial peptide, the antibacterial peptide is used for preparing an inhibitor against bacterial infection.

[0009] The bacteria are Vibrio harveyi or Micrococcus luteus.

[0010] An inhibitor against bacterial infection, containing an antibacterial peptide shown in the amino acid sequence in SEQ ID No. 1. BRIEF DESCRIPTION OF DRAWINGS

[0011] Figure 1The antibacterial peptide provided by the embodiment of the present application has a killing effect on bacteria. DETAILED DESCRIPTION

[0012] The present application is further described below in conjunction with examples. The examples are intended to exemplarily describe the present application, but not to limit the present application in any form.

[0013] Example 1

[0014] The antibacterial peptide of the present application (named PoP16) is the amino acid sequence in the sequence listing SEQ ID No. 1.

[0015] The sequence listing SEQ ID No. 1 is:

[0016] KKRRRGLSRFRMGRGY

[0017] (a) Sequence characteristics:

[0018] • Length: 16

[0019] • Type: Amino acid sequence

[0020] • Chain type: Single chain

[0021] • Topology: Linear

[0022] (b) Molecular type: Protein

[0023] (c) Hypothesis: No

[0024] (d) Antisense: No

[0025] (e) Original source: Artificial synthesis

[0026] Example 2

[0027] Application of the antibacterial peptide

[0028] Step 1) Preparation of bacteria.

[0029] Vibrio harveyi and Micrococcus luteus were cultured in LB medium to OD 600 0.8, and then centrifuged (5000g, 4℃) for 10 min. The bacterial bodies were collected and suspended in PBS to a final concentration of 5x10 3 cfu / ml.

[0030] The composition of the LB is as follows in terms of weight percentage: 1.0% proteose peptone, 0.5% yeast powder, 1.0% sodium chloride, 97.5% distilled water. The composition of the PBS is as follows in terms of weight percentage: 0.8% NaCl, 0.02% KCl, 0.358% Na2HPO4·12H2O, 0.024% NaH2PO4.

[0031] The Vibrio harveyi was preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No. 1985. The Micrococcus luteus was purchased from China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC 1.191 (https: / / cgmcc.net / directory / detail?cgmccl=1.191&number=&genus=&species=&yiming=%E8%97%A4%E9%BB%84%E8%8F%8C&page=1).

[0032] Step 2) The antibacterial effect of PoP16.

[0033] 100 ul of the Vibrio harveyi and Micrococcus luteus bacterial solution in step 1) above were mixed with antibacterial peptide PoP16 (final concentration of 20 μΜ) or PBS (control) respectively, and incubated at 22 °C for 4 h. Then the mixture was spread on LB solid plate, and the growth of Vibrio harveyi (A) and Micrococcus luteus (B) was observed after 32-48 h culture. Figure 1 A left) and Micrococcus luteus (B left) were observed. At the same time, colony counting was performed. The results showed that compared with the PBS treatment group, there were almost no Vibrio harveyi (A right) and Micrococcus luteus (B right) survived in the PoP16 treatment group, i.e. the bactericidal rate of PoP16 was about 100%. Figure 1 B right) were observed. At the same time, colony counting was performed. The results showed that compared with the PBS treatment group, there were almost no Vibrio harveyi (A right) and Micrococcus luteus (B right) survived in the PoP16 treatment group, i.e. the bactericidal rate of PoP16 was about 100%. Figure 1 B right) were observed. At the same time, colony counting was performed. The results showed that compared with the PBS treatment group, there were almost no Vibrio harveyi (A right) and Micrococcus luteus (B right) survived in the PoP16 treatment group, i.e. the bactericidal rate of PoP16 was about 100%. Figure 1 B right) were observed. At the same time, colony counting was performed. The results showed that compared with the PBS treatment group, there were almost no Vibrio harveyi (A right) and Micrococcus luteus (B right) survived in the PoP16 treatment group, i.e. the bactericidal rate of PoP16 was about 100%.

[0034] These results showed that PoP16 had high antibacterial effect, and could be applied to the prevention and treatment of bacterial diseases / infections.

Claims

1. An antimicrobial peptide, characterized in that: The sequence of the antimicrobial peptide is shown in SEQ ID No.

1.

2. An application of the antimicrobial peptide according to claim 1, characterized in that: The antimicrobial peptide is used to prepare inhibitors against bacterial infections; The bacteria is Vibrio harzianum ( Vibrio harveyi ) or Micrococcus luteus ( Micrococcus luteus ).

3. An inhibitor of antibacterial infection, characterized in that: Contains the antimicrobial peptide as described in claim 1; The bacteria is Vibrio harzianum ( Vibrio harveyi ) or Micrococcus luteus ( Micrococcus luteus ).

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