Almond polypeptide sunscreen and moisturizing skin care product and preparation method thereof

Abstract

The invention discloses an almond polypeptide sunscreen moisturizing skin care product and a preparation method thereof. The content of almond antioxidant peptide YLSF in the almond polypeptide sunscreen moisturizing skin care product is 2-8 mg / mL. During the preparation, water is used as a solvent, and almond antioxidant peptide YLSF is added to prepare the almond polypeptide sunscreen moisturizing skin care product. The almond polypeptide sunscreen moisturizing skin care product of the invention is added with bitter almond antioxidant peptide YLSF, which can effectively avoid the damage to the skin caused by ultraviolet radiation and has a good sunscreen moisturizing effect.

Description

Technical Field

[0001] The present invention relates to the technical field of sunscreen and skin care, in particular to an almond polypeptide sunscreen and moisturizing skin care product and a preparation method thereof. Background Art

[0002] With the aging of the population and the improvement of living standards, people have higher and higher requirements for health and beauty, and the demand for youthful skin has naturally become the focus. Therefore, anti-skin aging active ingredients are the best way to solve this problem. The key to delaying skin aging lies in the prevention and treatment of photoaging. Studies have shown that the main cause of photoaging is ultraviolet rays. Therefore, the main measure to avoid photoaging is to avoid excessive ultraviolet radiation while taking sun protection and post-sun repair. Skin photoaging starts with the degradation of collagen fibers, followed by skin relaxation, wrinkles, dehydration, hyaluronic acid loss, and pigment deposition. In response to these phenomena, the most important measures at present are sunscreens and antioxidants. Sunscreens are divided into physical sunscreens and chemical sunscreens. Physical sunscreens can block or scatter ultraviolet light to absorb ultraviolet rays of a specific wavelength or dissipate ultraviolet energy with other lower energy, such as dioxypeptides, chromium oxide, cobalt oxide, etc. Chemical sunscreens can block the EGF-phosphorylation pathway, scavenge free radicals, and reduce the content and activity of ROS, such as aminobenzoic acid derivatives and cinnamic acids. The skin cannot absorb two sunscreens, and due to different skin types, it is easy to cause skin sensitivity defects. Therefore, it is becoming increasingly important to develop natural products that have both the ability to absorb ultraviolet rays and the ability to resist oxidation.

[0003] Peptides are highly safe, have strong physiological activity, and good biocompatibility. They are very effective in improving photo-aged skin. Many studies have shown that small molecule peptides (200-1000KDa, 65%) are more effective than high molecular weight peptides (>1000KDa, 72%) and gelatin (>120KDa) in improving photo-aged skin. The molecular weight of collagen peptides affects their photo-aging protection. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to provide an almond polypeptide sunscreen and moisturizing skin care product with sunscreen and moisturizing functions and a preparation method thereof.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] Almond polypeptide sunscreen and moisturizing skin care product, the content of almond antioxidant peptide YLSF is 2-8 mg / mL.

[0007] The above-mentioned almond polypeptide sunscreen and moisturizing skin care product is composed of almond antioxidant peptides, moisturizers and water.

[0008] The almond polypeptide sunscreen and moisturizing skin care product consists of almond antioxidant peptide, moisturizer, emulsifier, co-emulsifier, moisturizer, thickener, preservative, neutralizer, skin conditioner, fragrance, whitening agent and water.

[0009] The almond polypeptide sunscreen and moisturizing skin care product, the moisturizer is one or a mixture of two or more of glycerin, 1,2-propylene glycol and cherry blossom extract.

[0010] In the almond polypeptide sunscreen and moisturizing skin care product, the amount of glycerin used is 2-5wt%, the amount of 1,2-propylene glycol used is 2-5wt%, and the amount of cherry blossom extract used is 0.5-1.5wt%.

[0011] In the almond polypeptide sunscreen and moisturizing skin care product, the cherry blossom extract consists of water, butylene glycol and Tokyo cherry blossom leaf extract, and the content of Tokyo cherry blossom leaf extract in the cherry blossom extract is 5-25wt%.

[0012] The above-mentioned almond polypeptide sunscreen and moisturizing skin care product, the emulsifier is cetearyl alcohol polyether-6 olive oil ester; the co-emulsifier is cetearyl alcohol; the emollient is one or two or a mixture of more than two of caprylic / capric triglyceride, ethylhexyl palmitate, tocopheryl acetate and almond oil; the thickener is carbomer; the preservative is one or two or a mixture of more than two of methylparaben, phenoxyethanol and ethylhexylglycerin; the neutralizer is triethanolamine; the skin conditioning agent is one or two or a mixture of more than two of bisabolol, ginger root extract, ophiopogon extract, opuntia cochinchinensis stem extract, sophora flavescens root extract and angelica extract; the fragrance is essence; the whitening agent is titanium dioxide and / or α-arbutin.

[0013] The above almond polypeptide sunscreen moisturizing skin care product, the amount of cetearyl alcohol polyether-6 olive oil ester is 1.5-2.5wt%; the amount of cetearyl alcohol is 0.05-0.15wt%; the amount of caprylic / capric triglyceride is 1.5-2.5wt%, the amount of ethylhexyl palmitate is 1.5-2.5wt%, the amount of tocopherol acetate is 0.25-0.35wt%, the amount of almond oil is 0.4-0.55wt%; the amount of carbomer is 0.05-0.15wt%; the amount of methylparaben is 0.05-0.1wt%, the amount of phenoxyethanol is 0.05-0.1wt%; the amount of ethylhexylglycerin is The amount of triethanolamine is 0.05-0.1wt%; the amount of bisabolol is 0.05-0.1wt%, the amount of ginger root extract is 0.1-0.15wt%, the amount of ophiopogon extract is 0.1-0.15wt%, the amount of twisted cactus stem extract is 0.1-0.15wt%, the amount of sophora flavescens root extract is 0.1-0.15wt%, and the amount of angelica extract is 0.2-0.3wt%; the amount of essence is 0.01-0.15wt%, the amount of titanium dioxide is 0.5-1.0wt%, and the amount of α-arbutin is 0.3-0.5wt%.

[0014] A method for preparing an almond polypeptide sunscreen and moisturizing skin care product comprises taking water as a solvent and adding almond antioxidant peptide YLSF to prepare the almond polypeptide sunscreen and moisturizing skin care product.

[0015] The preparation method of the almond polypeptide sunscreen and moisturizing skin care product comprises the following steps:

[0016] (1) adding an emulsifier, a co-emulsifier and an emollient into an emulsifying pot, heating to 75° C., mixing and melting to obtain an emulsion;

[0017] (2) adding a thickener, a humectant and water into a water phase pot, heating to 75° C. and mixing until uniformly dispersed to obtain a water phase liquid;

[0018] (3) The aqueous phase liquid is pumped into an emulsifying pot, vacuum homogenized for 3 min and cooled to obtain a mixed dispersion A;

[0019] (4) When the temperature drops to 60° C., add a preservative, a neutralizer and a skin conditioner to the mixed dispersion A, and stir to mix evenly to obtain a mixed dispersion B;

[0020] (5) When the temperature drops to 45° C., a fragrance and a whitening agent are added to the mixed dispersion B, and after mixing evenly, a mixed dispersion C is obtained;

[0021] (6) Add almond antioxidant peptide YLSF to the mixed dispersion C and mix well, then cool to 40°C and discharge.

[0022] The technical solution of the present invention achieves the following beneficial technical effects:

[0023] The almond polypeptide sunscreen moisturizing skin care product of the present invention is added with bitter almond antioxidant peptide YLSF, and by adding a specific proportion of emollients, skin conditioners, etc., it can effectively avoid the damage to the skin caused by ultraviolet radiation. The almond polypeptide sunscreen moisturizing skin care product is used for mouse skin protection. The results show that when the dosage of bitter almond antioxidant peptide in the almond polypeptide sunscreen moisturizing skin care product reaches 8 mg / mL, small focal scabs are occasionally seen in the mouse skin tissue, the epidermis is intact and thickened, and keratinization repair can be seen; scattered granulocyte infiltration can be seen from the dermis to the subcutaneous tissue, and a large number of hair follicles are generated and scattered. It shows that the almond polypeptide sunscreen moisturizing skin care product added with bitter almond antioxidant peptide has a good sunscreen and moisturizing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1a Actual photos of the skin appearance of mice in the blank group A in the embodiment of the present invention;

[0025] Figure 1b Actual photos of the skin appearance of mice irradiated with UV light in the control group B of the present invention;

[0026] Figure 1c Actual photos of the skin appearance of mice irradiated with UV light in the low-dose group C of the present invention;

[0027] Figure 1d Actual photos of the skin appearance of mice irradiated with UV light in the medium dose group D in the embodiment of the present invention;

[0028] Figure 1e Actual photos of the skin appearance of mice irradiated with UV light in the high-dose group E in the embodiment of the present invention;

[0029] Figure 2a HE staining section of mouse skin in the blank group A in the present invention (2.0X);

[0030] Figure 2b HE staining section of mouse skin in blank group A in the present invention (20.0X);

[0031] Figure 3a HE staining section of mouse skin in control group B in the present invention (2.0X);

[0032] Figure 3b HE staining section of mouse skin in control group B in the present invention (20.0X);

[0033] Figure 4a HE staining section of mouse skin in low-dose group C in the present invention (2.0X);

[0034] Figure 4b HE staining section of mouse skin in low-dose group C in the present invention (20.0X);

[0035] Figure 5a HE staining section of mouse skin in the medium dose group D in the present invention (2.0X);

[0036] Figure 5b HE staining section of mouse skin in the medium dose group D in the present invention (20.0X);

[0037] Figure 6 HE staining section of mouse skin in the E high-dose group in the present invention (2.0X);

[0038] Figure 7 HE staining section of mouse skin in the high-dose group E in the example of the present invention (20.0X). DETAILED DESCRIPTION

[0039] 1. Almond polypeptide sunscreen moisturizing skin care products

[0040] The other components of the almond polypeptide sunscreen moisturizing skin care product in this embodiment except the bitter almond antioxidant peptide are shown in Table 1. "%" represents the mass percentage of the component in the almond polypeptide sunscreen moisturizing skin care product, and "mg / mL" represents the mass volume content of the component in the almond polypeptide sunscreen moisturizing skin care product, that is, the mass concentration.

[0041] Table 1

[0042]

[0043] In Table 1: OLIVEM 800, cetostearyl alcohol, GTCC, 2-EHP and VE acetate were purchased from Shanghai Shangde Chemical Co., Ltd.; Carbopol 941 was purchased from Shanghai Zhaoheng Industrial Co., Ltd.; glycerin and propylene glycol were purchased from Shanghai Dike Fine Chemical Co., Ltd.; methylparaben was purchased from Shanghai Qianyi Chemical Co., Ltd.; triethanolamine was purchased from Nanjing Gutian Chemical Co., Ltd.; PE9010 was purchased from Shanghai Saifu Chemical Development Co., Ltd.; Xinminshu was purchased from Shanghai Yuanshang Science and Technology Trade Co., Ltd.; plant allergy-relieving agent was purchased from Miaosen International Co., Ltd., and the content of Ophiopogon japonicus extract in the plant allergy-relieving agent was 3-6wt%; cherry blossom extract was purchased from Xianglin Enterprise Co., Ltd., and the content of Tokyo cherry blossom leaf extract in the cherry blossom extract was 5-25wt%; angelica extract was purchased from Xier Cosmetics Co., Ltd., and the content of angelica extract in the angelica extract was 10-35wt%.

[0044] The production process of the almond polypeptide sunscreen and moisturizing skin care product in this embodiment is as follows:

[0045] (1) adding emulsifier No. 1 raw material, co-emulsifier No. 2 raw material, and emollient No. 3-6 raw materials into an emulsifying pot, heating to 75° C., and stirring until completely melted to obtain an emulsion;

[0046] (2) Adding solvent No. 7, thickener No. 8, humectants 9-10 and No. 17 into a water phase pot, heating to 75° C., stirring until the mixture is uniform and completely dissolved, to obtain a water phase liquid;

[0047] (3) The aqueous phase liquid in the aqueous phase pot is pumped into the emulsification pot to mix with the emulsion, and the vacuum is maintained. After homogenization for 3 minutes, the temperature is lowered;

[0048] (4) Cooling to 60° C., adding preservatives No. 11 and No. 13, neutralizer No. 12, skin conditioners No. 14-15 and No. 18, and stirring to mix well;

[0049] (5) Continue to cool to 45° C., add the aromatic raw material No. 16 and the whitening agent raw materials No. 19-20, and stir evenly to obtain a mixed dispersion; add the bitter almond antioxidant peptide YLSF No. 21 to the mixed dispersion, the amino acid sequence of the bitter almond antioxidant peptide YLSF can be found in the polypeptide named YLSF in Table 1 of the embodiment of patent CN115124594A, and stir to mix evenly;

[0050] (6) Cool down to 40℃ and discharge.

[0051] The above-mentioned raw material mixing method and mixing order can retain the effectiveness of each component to the maximum extent compared with other mixing methods and mixing orders, and prepare an almond polypeptide sunscreen and moisturizing skin care product with good stability and good sunscreen and moisturizing effects.

[0052] The almond polypeptide sunscreen moisturizing skin care product in this embodiment has a milky white appearance and a pH value of 6.3. The almond polypeptide sunscreen moisturizing skin care product is recorded as ZXR-P1, and the content of bitter almond antioxidant peptide in ZXR-P1 is 2 mg / mL.

[0053] Almond peptide sunscreen and moisturizing skin care products ZXR-P2 and ZXR-P3 were prepared according to the formula in Table 1 and the above method; the only difference between ZXR-P2 and ZXR-P3 and ZXR-P1 was that the content of bitter almond antioxidant peptide was different, which was 4 mg / mL and 8 mg / mL, respectively.

[0054] 2. Evaluation of the effect of almond polypeptide sunscreen and moisturizing skin care products

[0055] 2.1 Protective effect of ZXR-P on UV radiation mouse skin damage model

[0056] Grouping and hair removal: 30 C57BL / 6 mice were randomly divided into 5 groups: Group A (blank control group), Group B (UVB radiation group), Group C (2mg / mL low-dose group, i.e., applied with ZXR-P1), Group D (4mg / mL medium-dose group, i.e., applied with ZXR-P2), and Group E (8mg / mL high-dose group, i.e., applied with ZXR-P3), and ear tags were tagged. After one week of feeding, the biological clock of the mice was adjusted (active during the day and rest at night). Draw the curtains during the day and turn on the lights at night. Change the water every two days and the cage every four days. Remove the hair in the middle of the mouse's back. The hair removal range: 3*2.5cm on the back. 2 .

[0057] Irradiation dose: Mice in group A were blank controls and received no treatment. Mice in group B were irradiated with 180 mJ / cm 2 The mice in groups C, D and E were irradiated for 30 minutes (400 μL each) and then treated as in group B.

[0058] Histomorphological evaluation: The mice were sacrificed, and the dorsal skin was removed and trimmed into 0.5×0.5 cm tissue blocks, which were fixed with 4% paraformaldehyde for 24 h.

[0059] HE staining: Dewaxing xylene I and II for 10 minutes each. Then soak in 100% (I, II), 90%, 80%, 70% alcohol by volume for 5 minutes each, rinse with running water for 5 minutes; then stain with hematoxylin for 5 minutes, rinse with running water; then add 5% acetic acid for differentiation for 1 minute, until the tissue is fully covered, rinse with running water; then stain with eosin for 1 minute, rinse with running water; then soak in 70%, 80%, 90%, 100% alcohol by volume for 10 seconds each, and finally soak in xylene for 1 minute, dry naturally. Add neutral gum (one drop is enough, the tissue should be completely covered after pressing, and avoid the generation of bubbles), and seal the slide.

[0060] 2.2 Preparation and reading of pathological slides

[0061] 4% paraformaldehyde can be used to fix the samples. After the fixation state is at a good level, it is necessary to further test the qualified samples of trimming, dehydration, embedding, staining, slicing, sealing and microscopic examination according to the SOP procedure of the unit's pathological experiment. Carefully browse the slices and digital slices under a microscope, carefully observe the specific conditions of the tissue slices at the corresponding magnification, and complete a detailed description of the basic pathological changes in the slices in text form, mainly including descriptions of various conditions such as congestion, hemorrhage, edema, necrosis, degeneration, hyperplasia, fibrosis, organization, inflammatory changes, etc., and reflect the differences between the slices. Image the typical lesions and mark them with arrows.

[0062] 3. Results and analysis

[0063] 3.1 Mouse skin pathological sections

[0064] 3.1.1 Effects on mouse skin appearance

[0065] Figures 1a to 1e The following are the actual photos of the skin appearance of mice in A blank group, B control group, C low-dose group, D medium-dose group and E high-dose group. Figures 1a to 1e It can be seen that under the same UV power and radiation conditions, with the increase of ZXR-P dosage, the degree of mouse skin damage gradually decreased. The blank group was pure white, and the skin of the mice in the control group without ZXR-P was completely burnt yellow, with obvious burns and severe UV damage; the skin of the mice in the low-dose group was yellow and completely burned; the color of the medium-dose group became lighter, and some white areas appeared, and the high-dose group had a small amount of burns, which had a significant effect in inhibiting UV damage.

[0066] 3.1.2 Evaluation of HE staining

[0067] A blank group mice ( Figure 2a and Figure 2b ) The epidermis of the skin tissue is intact, with clear structure and visible keratinized layer; the collagen fibers in the dermis are neatly arranged, and the skin appendages, hair follicles and sebaceous glands are scattered; the subcutaneous tissue is located below the dermis, and is composed of loose connective tissue, adipose tissue and muscle layer, with no obvious abnormalities.

[0068] B control group mice ( Figure 3a and Figure 3b ) In the skin tissue, large areas of epidermal necrosis, structural disappearance, nuclear fragmentation, and a large number of inflammatory cell infiltration (black arrow) can be seen; in the superficial dermis, a large amount of connective tissue necrosis and nuclear fragmentation (blue arrow) can be seen, accompanied by a large number of granulocyte infiltration (green arrow), and a large number of hair follicles are necrotic and the structure disappears; in the subcutaneous tissue, a large amount of connective tissue necrosis can be seen, accompanied by a large number of inflammatory cell infiltration (yellow arrow), and the muscle layer is intact, with no obvious abnormalities.

[0069] 2mg / mL low dose group C mice ( Figure 4a to Figure 4b ) In the skin tissue, large areas of epidermal necrosis, structural disappearance, nuclear fragmentation, and a large number of inflammatory cell infiltration (black arrow) can be seen; in the dermis, a large amount of connective tissue necrosis and nuclear fragmentation (blue arrow) can be seen, accompanied by a large number of granulocyte infiltration (green arrow), and a large number of hair follicles are necrotic and the structure disappears; in the subcutaneous tissue, a large amount of connective tissue necrosis can be seen, accompanied by a large number of inflammatory cell infiltration (yellow arrow), and the muscle layer is intact, with no obvious abnormalities.

[0070] 4mg / mL medium dose group D ( Figure 5a to Figure 5b) In the skin tissue, a small area of ​​epidermal necrosis and shedding, structural disappearance, nuclear fragmentation, and a small amount of inflammatory cell infiltration (black arrow) can be seen; scattered granulocyte infiltration (red arrow) can be seen in the superficial dermis, and a small amount of hair follicle necrosis with structural disappearance; a large amount of connective tissue necrosis can be seen in the subcutaneous tissue, accompanied by a large amount of inflammatory cell infiltration (yellow arrow), and the muscle layer is intact, with no obvious abnormalities.

[0071] 8mg / mL high dose E group ( Figure 6 to Figure 7 ) Occasionally, small focal scabs (black arrows) are seen in the skin tissue, the epidermis is intact and thickened, and keratinization repair (blue arrows) can be seen; scattered granulocyte infiltration (yellow arrows) can be seen from the dermis to the subcutaneous tissue, and a large number of hair follicles are generated and scattered; the muscle layer is intact, and no obvious abnormalities are seen. It can be seen that the almond polypeptide sunscreen and moisturizing skin care product in this embodiment has a good sunscreen and moisturizing effect, and the sunscreen and moisturizing skin care effect is better as the dosage increases.

Claims

1. Almond polypeptide sunscreen moisturizing skin care product, characterized in that: The content of almond antioxidant peptide YLSF is 2-8 mg / mL; it is composed of almond antioxidant peptide, moisturizer, emulsifier, auxiliary emulsifier, emollient, thickener, preservative, neutralizer, skin conditioner, fragrance, whitening agent and water; The moisturizer is one or a mixture of two or more of glycerin, 1,2-propylene glycol and cherry blossom extract; The emulsifier is ceteareth-6 olive oil; the co-emulsifier is cetearyl alcohol; the emollient is one or a mixture of two or more of caprylic / capric triglyceride, ethylhexyl palmitate, tocopheryl acetate and almond oil; the thickener is carbomer; the preservative is one or a mixture of two or more of methylparaben, phenoxyethanol and ethylhexylglycerin; the neutralizer is triethanolamine; the skin conditioning agent is one or a mixture of two or more of bisabolol, ginger root extract, ophiopogon extract, opuntia cochinchinensis stem extract, sophora flavescens root extract and angelica extract; the fragrance is essence; the whitening agent is titanium dioxide and / or α-arbutin.

2. The almond polypeptide sunscreen and moisturizing skin care product according to claim 1, characterized in that: The amount of glycerol used is 2-5wt%, the amount of 1,2-propylene glycol used is 2-5wt%, and the amount of cherry blossom extract used is 0.5-1.5wt%.

3. The almond polypeptide sunscreen and moisturizing skin care product according to claim 1, characterized in that: The cherry blossom extract consists of water, butylene glycol and Tokyo cherry blossom leaf extract, and the content of Tokyo cherry blossom leaf extract in the cherry blossom extract is 5-25wt%.

4. The almond polypeptide sunscreen and moisturizing skin care product according to claim 1, characterized in that: The dosage of cetearyl alcohol polyether-6 olive oil ester is 1.5-2.5wt%; the dosage of cetearyl alcohol is 0.05-0.15wt%; the dosage of caprylic / capric triglyceride is 1.5-2.5wt%, the dosage of ethylhexyl palmitate is 1.5-2.5wt%, the dosage of tocopherol acetate is 0.25-0.35wt%, the dosage of almond oil is 0.4-0.55wt%; the dosage of carbomer is 0.05-0.15wt%; the dosage of methylparaben is 0.05-0.1wt%, the dosage of phenoxyethanol is 0.05-0.1wt%; the dosage of ethylhexylglycerol is 0.05- 0.1wt%; the amount of triethanolamine is 0.05-0.1wt%; the amount of bisabolol is 0.05-0.1wt%, the amount of ginger root extract is 0.1-0.15wt%, the amount of ophiopogon extract is 0.1-0.15wt%, the amount of twisted prickly cactus stem extract is 0.1-0.15wt%, the amount of sophora flavescens root extract is 0.1-0.15wt%, and the amount of angelica extract is 0.2-0.3wt%; the amount of essence is 0.01-0.15wt%, the amount of titanium dioxide is 0.5-1.0wt%, and the amount of α-arbutin is 0.3-0.5wt%.

5. A method for preparing an almond polypeptide sunscreen and moisturizing skin care product, characterized in that: The almond polypeptide sunscreen and moisturizing skin care product as claimed in claim 1 is prepared by using water as a solvent and adding almond antioxidant peptide YLSF.

6. The method for preparing the almond polypeptide sunscreen and moisturizing skin care product according to claim 5, characterized in that: The steps include: (1) adding an emulsifier, a co-emulsifier and an emollient into an emulsifying pot, heating to 75° C., mixing and melting to obtain an emulsion; (2) adding a thickener, a humectant and water into a water phase pot, heating to 75° C. and mixing until uniformly dispersed to obtain a water phase liquid; (3) The aqueous phase liquid is pumped into an emulsifying pot, vacuum homogenized for 3 min and cooled to obtain a mixed dispersion A; (4) When the temperature drops to 60° C., add a preservative, a neutralizer and a skin conditioner to the mixed dispersion A, and stir to mix evenly to obtain a mixed dispersion B; (5) When the temperature drops to 45° C., a fragrance and a whitening agent are added to the mixed dispersion B, and after mixing evenly, a mixed dispersion C is obtained; (6) Add almond antioxidant peptide YLSF to the mixed dispersion C and mix well, then cool to 40°C and discharge.

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