A solid-liquid mixed veterinary drug preparation and preparation method thereof

Abstract

The present invention relates to the technical field of Chinese veterinary drug preparations, and proposes a solid-liquid mixed veterinary drug preparation and a preparation method thereof, wherein the raw materials of the veterinary drug preparation include plant-derived Chinese medicinal materials, cellulase, acid protease, and pectinase. Through the above technical scheme, the main components of the plant-derived Chinese medicinal materials are extracted, and the original medicinal materials are retained. The prepared solid-liquid mixed preparation is easier to absorb, and the problem that the preparation process of the Chinese veterinary drug preparation in the prior art is complicated and the effect on the prevention and treatment of chicken synoviae Mycoplasma is poor is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese veterinary drug preparations, and in particular to a solid-liquid mixed veterinary drug preparation and a preparation method thereof. Background Art

[0002] With the serious situation of antibiotic use, antibiotic-free and effective Chinese veterinary medicine has become one of the main means to solve animal-derived food safety and public health safety issues. Chinese veterinary medicine preparations can replace the role of antibiotics, will not produce drug resistance, will not have drug residues, and are safe.

[0003] However, Chinese veterinary medicine preparations not only have complex production processes, but also high energy consumption and production costs, and are insufficient in treating intestinal diseases and promoting growth in broilers. For example, powders are made by directly crushing Chinese medicinal materials. Its characteristic is that it retains the medicinal properties of the original Chinese medicine, but after use, it reaches the intestines of poultry animals and has a short residence time, cannot be fully absorbed, and has poor effects on preventing and treating poultry viral diseases, bacterial diseases, and mycoplasma diseases; granules are made by extracting Chinese medicinal materials and making the extract into preparations. Its characteristic is that the effective ingredients of Chinese medicine are extracted, but the preparation process is cumbersome and increases costs. Summary of the invention

[0004] The present invention provides a solid-liquid mixed veterinary drug preparation and a preparation method thereof, which solves the problems in the related art that the preparation process of the veterinary drug preparation is complicated and the effect on preventing and treating Mycoplasma synoviae is poor.

[0005] The technical solution of the present invention is as follows:

[0006] A solid-liquid mixed veterinary drug preparation, the raw materials of which include plant-derived Chinese medicinal materials, cellulase, acid protease and pectinase.

[0007] As a further technical solution, the raw materials of the veterinary preparation include the following components by weight: 50-60 parts of plant-derived Chinese medicinal materials, 20-30 parts of cellulase, 20-30 parts of acid protease, and 20-30 parts of pectinase.

[0008] As a further technical solution, the plant-derived Chinese medicinal materials include one or more of purslane, psoralea corylifolia, drynaria, honeysuckle, cornus officinalis, schisandra chinensis, and perilla leaves.

[0009] The present invention also includes a method for preparing a solid-liquid mixed veterinary drug preparation, comprising the following steps:

[0010] S1, breaking the wall of the plant-derived Chinese medicinal materials to obtain pre-treated plant-derived Chinese medicinal materials;

[0011] S2, enzymatically hydrolyzing the pretreated plant-derived Chinese medicinal materials to obtain an enzymatic hydrolysis mixture;

[0012] S3. Extracting the enzymatic hydrolysis mixture with an organic solvent to obtain a solid-liquid mixed veterinary drug preparation.

[0013] As a further technical solution, the wall breaking in S1 is to grind the plant-derived Chinese medicinal materials at a speed of 2000-5000 r / min for 5-10 minutes, add water to control the grinding temperature to 75-85°C, and continue to grind at a speed of 3000-5500 r / min for 1-2 hours.

[0014] As a further technical solution, the mass ratio of the plant-derived Chinese medicinal materials to water in S1 is 1:1-2.

[0015] As a further technical solution, the enzymatic hydrolysis in S2 is to hydrolyze the pretreated plant-derived Chinese medicinal materials with cellulase, acid protease and pectinase in sequence.

[0016] As a further technical solution, the mass concentration of the cellulase is 0.1-0.9%.

[0017] As a further technical solution, the mass concentration of the acidic protease is 0.015-0.05%.

[0018] As a further technical solution, the enzymatic hydrolysis in S2 is to add cellulase to the pretreated plant-derived Chinese medicinal materials, and perform enzymatic hydrolysis at pH 4-5 and temperature of 55-60°C for 0.5-1.5h; then add acid protease, perform enzymatic hydrolysis at 50-52°C for 0.5-1.5h, and finally add pectinase, perform enzymatic hydrolysis at 46-49°C for 0.5-1.5h to obtain an enzymatic hydrolysis mixture.

[0019] The working principle and beneficial effects of the present invention are:

[0020] 1. The newly developed solid-liquid mixed preparation of the present invention is mainly composed of plant-derived Chinese medicinal materials and cellulase, acid protease, and pectinase. The prepared veterinary preparation not only extracts the main components of plant-derived Chinese medicinal materials but also retains the original medicinal materials. Due to the particularity of the intestines of livestock and poultry, the solid-liquid mixed preparation is absorbed faster than powders and granules, can resist viruses, improve immunity, relieve fever and relieve pain, and enhance intestinal immunity, while reducing the problems of antibiotic residues, drug resistance and side effects, and the effective rate against chicken synoviae Mycoplasma is as high as 96.7%.

[0021] 2. In the present invention, after the plant-derived Chinese medicinal materials are broken, cellulase, acid protease, and pectinase are added for enzymolysis, which can enhance the release of effective ingredients, degrade macromolecular substances, promote digestion and absorption, and improve the taste of the preparation. After the enzymolysis mixture is extracted with an organic solvent, the extraction rate is increased by 50%, the intestinal absorption is good, and the volatilization of volatile components is reduced (enzymolysis + low-boiling point solvent immersion extraction), thereby resulting in a higher curative effect of the obtained solid-liquid mixed preparation. After the organic solvent extraction, the organic solvent is removed to obtain a veterinary preparation, and the preparation process is simple, the process conditions are mild, and it is easy to control. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] S1, crushing 500g of purslane at a speed of 2000r / min for 5min, then adding 500g of water and controlling the temperature to 79-81°C and continuing to crush at a speed of 3000r / min for 1h to obtain pretreated purslane;

[0025] S2, adding 200g 0.9% cellulase to the pretreated purslane, and performing constant temperature enzymolysis at pH 4 and temperature 55°C for 1.5h to obtain enzymolysate I; adding 200g 0.05% acid protease to enzymolysate I, and performing constant temperature enzymolysis at temperature 50°C for 1.5h to obtain enzymolysate II; adding 200g 0.015% pectinase to enzymolysate II, and performing constant temperature enzymolysis at 46°C for 1.5h to obtain an enzymolysis mixture;

[0026] S3. Add 500 g of ethanol and 200 g of methanol to the enzymatic hydrolysis mixture and soak for 20 min. After the soaking, remove the ethanol and methanol by vacuum distillation to obtain a solid-liquid mixed veterinary drug preparation.

[0027] Example 2

[0028] S1. Grind 550 g of purslane at 3000 r / min for 7 min, then add 1100 g of water to control the temperature to 75-78 ° C, and continue to grind at 4000 r / min for 1.5 h;

[0029] S2, adding 250g of 0.5% cellulase to the pretreated purslane, and performing constant temperature enzymolysis at pH 5 and temperature 60°C for 1.5h to obtain enzymolysate I; adding 250g of 0.25% acid protease to enzymolysate I, and performing constant temperature enzymolysis at temperature 52°C for 0.5h to obtain enzymolysate II; adding 250g of 0.015% pectinase to enzymolysate II, and performing constant temperature enzymolysis at 49°C for 0.5h to obtain an enzymolysis mixture;

[0030] S3. Add 550 g of ethanol and 250 g of methanol to the enzymatic hydrolysis mixture and soak for 20 min. After soaking, remove the ethanol and methanol by vacuum distillation to obtain a solid-liquid mixed veterinary drug preparation.

[0031] Example 3

[0032] S1. Crush 600g of purslane at 5000r / min for 10min, then add 900g of water and control the temperature to 80-85°C, and continue to crush at 5500r / min for 2h;

[0033] S2, adding 300g of 0.1% cellulase to the pretreated purslane, and performing constant enzymolysis at a pH of 4.5 and a temperature of 58°C for 1h to obtain enzymolysate I; adding 300g of 0.015% acid protease to the enzymolysate I, and performing constant enzymolysis at a temperature of 51°C for 1h to obtain enzymolysate II; adding 300g of 0.015% pectinase to the enzymolysate II, and performing constant enzymolysis at 47°C for 1h to obtain an enzymolysis mixture;

[0034] S3. Add 600 g of ethanol and 300 g of methanol to the enzymatic hydrolysis mixture and soak for 20 min. After the soaking, remove the ethanol and methanol by vacuum distillation to obtain a solid-liquid mixed veterinary drug preparation.

[0035] Example 4

[0036] Compared with Example 1, in Example 4, Purslane is replaced with an equal amount of Psoralea corylifolia, and the rest is the same as in Example 1.

[0037] Example 5

[0038] Compared with Example 1, in Example 5, Purslane is replaced with an equal amount of Rhizoma Drynariae, and the rest is the same as Example 1.

[0039] Example 6

[0040] Compared with Example 1, in Example 6, Purslane is replaced with an equal amount of Honeysuckle, and the rest is the same as Example 1.

[0041] Comparative Example 1

[0042] Compared with Example 1, the difference of Comparative Example 1 lies in S3. In Comparative Example 1, S3 is: adding 500 g ethanol and 200 g methanol to the enzymatic hydrolysis mixture and soaking for 20 minutes. After the soaking, the ethanol and methanol are removed by reduced pressure distillation, and then filtered to obtain the liquid to obtain a liquid veterinary preparation.

[0043] Comparative Example 2

[0044] Compared with Example 1, the difference of Comparative Example 2 lies in S3. In Comparative Example 2, S3 is: adding 500 g of ethanol and 200 g of methanol to the enzymatic hydrolysis mixture and soaking for 20 minutes. After the soaking, the ethanol and methanol are removed by reduced pressure distillation, and then filtered to obtain a solid to obtain a solid veterinary preparation.

[0045] Comparative Example 3

[0046] Compared with Example 1, the difference of Comparative Example 3 lies in S2. In Comparative Example 3, S2 is: adding 200 g of 0.9% cellulase to the pretreated purslane, and performing constant temperature enzymolysis at pH 4 and temperature 55°C for 1.5 hours to obtain enzymolysate I; adding 200 g of 0.05% acid protease to the enzymolysate I, and performing constant temperature enzymolysis at temperature 50°C for 1.5 hours to obtain an enzymolysis mixture.

[0047] Test example

[0048] 600 sick chickens diagnosed with Mycoplasma synoviae were collected from farmers in Zhao County, Shijiazhuang City, and randomly divided into 10 groups, 60 in each group, and the veterinary preparations prepared in Examples 1-6 and Comparative Examples 1-3 were respectively used to gavage the sick chickens for 3 days, the dosage of the veterinary preparation was 5 g / time (the veterinary preparation was stirred evenly before use), 2 times / d, and a blank control group (no drug was used to treat the sick chickens) was set, and the cured sick chickens and effective sick chickens were judged according to the following efficacy judgment, and the number of cured sick chickens and effective sick chickens was counted;

[0049] Efficacy judgment:

[0050] Cured: Leg joints returned to normal, other symptoms disappeared, and diet became normal;

[0051] Effective: In addition to being cured, symptoms are alleviated, the legs are improved, and the diet is restored.

[0052] The statistical results are shown in Table 1.

[0053] Table 1 Results of efficacy test on Mycoplasma synoviae

[0054]

[0055]

[0056] Compared with Example 1, the veterinary preparation obtained in Comparative Example 1 is the liquid part of the solid-liquid mixed veterinary preparation in Example 1, and the veterinary preparation obtained in Comparative Example 2 is the solid part of the solid-liquid mixed veterinary preparation in Example 1. As a result, the cure rate and effectiveness rate of Comparative Examples 1 and 2 for Mycoplasma synoviae were lower than those in Example 1, indicating that the therapeutic effect of preparing a solid-liquid mixed veterinary preparation by mixing solid and liquid on Mycoplasma synoviae is better than using only the liquid part or the solid part in the solid-liquid mixed veterinary preparation.

[0057] Compared with Example 1, Comparative Example 3 did not use pectinase for enzymolysis. As a result, the therapeutic effect of the solid-liquid mixed veterinary preparation prepared in Comparative Example 3 was lower than that in Example 1, indicating that the present invention uses cellulase, acid protease and pectinase to enzymolyze the pretreated plant-based Chinese medicinal materials, which can improve the cure rate and effectiveness of Mycoplasma synoviae.

[0058] Compared with Example 1, Example 4 replaces purslane with an equal amount of psoralea corylifolia, Example 5 replaces purslane with an equal amount of drynariae, and Example 6 replaces purslane with an equal amount of honeysuckle. As shown in Table 1, when the plant-derived Chinese medicinal material is purslane, the therapeutic effect on Mycoplasma synoviae is the best.

[0059] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A solid-liquid mixed veterinary preparation for treating Mycoplasma synoviae, characterized in that: The raw materials of the veterinary drug preparation include plant-derived Chinese medicinal materials, cellulase, acid protease, and pectinase; The plant-derived Chinese medicinal material is purslane; The preparation method of the solid-liquid mixed veterinary drug preparation for treating Mycoplasma synoviae comprises the following steps: S1, breaking the wall of the plant-derived Chinese medicinal materials to obtain pre-treated plant-derived Chinese medicinal materials; S2, enzymatically hydrolyzing the pretreated plant-derived Chinese medicinal materials to obtain an enzymatic hydrolysis mixture; S3, extracting the enzymatic hydrolysis mixture with an organic solvent to obtain a solid-liquid mixed veterinary drug preparation; The enzymatic hydrolysis in S2 is to hydrolyze the pretreated plant-derived Chinese medicinal materials with cellulase, acid protease and pectinase in sequence; The raw materials of the veterinary drug preparation include the following components by weight: 50-60 parts of plant-derived Chinese medicinal materials, 20-30 parts of cellulase, 20-30 parts of acid protease, and 20-30 parts of pectinase; The enzymatic hydrolysis in S2 is to add cellulase to the pretreated plant-derived Chinese medicinal materials, and perform enzymatic hydrolysis at pH 4-5 and temperature of 55-60° C. for 0.5-1.5 h; then add acid protease, perform enzymatic hydrolysis at 50-52° C. for 0.5-1.5 h, and finally add pectinase, perform enzymatic hydrolysis at 46-49° C. for 0.5-1.5 h to obtain an enzymatic hydrolysis mixture; The organic solvent in S3 consists of ethanol and methanol.

2. A solid-liquid mixed veterinary preparation for treating Mycoplasma synoviae according to claim 1, characterized in that: The wall breaking in S1 is to grind the plant-derived Chinese medicinal materials at a rotation speed of 2000-5000 r / min for 5-10 minutes, add water to control the grinding temperature to 75-85° C., and continue to grind at a rotation speed of 3000-5500 r / min for 1-2 hours.

3. A solid-liquid mixed veterinary preparation for treating Mycoplasma synoviae according to claim 2, characterized in that: The mass ratio of the plant-derived Chinese medicinal materials to water in S1 is 1:1-2.

4. The solid-liquid mixed veterinary preparation for treating Mycoplasma synoviae according to claim 1, characterized in that: The mass concentration of the cellulase is 0.1-0.9%.

5. A solid-liquid mixed veterinary preparation for treating Mycoplasma synoviae according to claim 1, characterized in that: The mass concentration of the acidic protease is 0.015-0.05%.

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