Seed dressing and spreading method of combining biostimulants and flutriafol

Abstract

The present invention relates to a method for applying a mixture of a biostimulant and flutriafol for seed dressing or broadcasting, wherein the biostimulant and flutriafol are first prepared into a mixture according to an active ingredient mass ratio of 1-50:1-10, and then the mixture is used for seed dressing or broadcasting; the seed dressing refers to preparing the mixture of the biostimulant and flutriafol into a seed dressing agent or other dosage forms suitable for seed dressing, and then applying the seed dressing; the broadcasting refers to preparing the mixture of the biostimulant and flutriafol into granules or other dosage forms suitable for mixing with fertilizers and soil for broadcasting, and then broadcasting. Compared with the prior art, the present invention can achieve the ultimate purpose of jointly improving the effect of preventing and controlling crop diseases, reducing the amount of medicine used, extending the duration of effect, delaying the generation of drug resistance, reducing the cost of disease prevention and control, and reducing the damage of pesticides to the environment.

Description

Technical Field

[0001] The invention relates to the technical field of application of pesticides or fertilizers, and in particular to an application method for combining a mixture of a biostimulant and flutriafol for seed dressing or spreading. Background Art

[0002] The application of agrochemicals can at least avoid 10-40% loss of agricultural output. However, there are still problems of excessive and improper use of agrochemicals in the agricultural application process.

[0003] As a broad-spectrum systemic fungicide, flutriafol has good protective and therapeutic effects on a variety of diseases caused by basidiomycetes and ascomycetes, and is often used to prevent and control powdery mildew, rust, smut, etc. Although flutriafol currently has a good control effect, it is well known that if flutriafol is used continuously and exclusively, the sensitivity of pathogens to flutriafol will decrease, and its resistance will increase, resulting in a gradual decrease in control effect; and if it is improperly used or overused, it will not only have a negative impact on the growth and yield of crops, but also increase the toxicity of flutriafol to the environment, and ultimately affect human health. Summary of the invention

[0004] The present invention aims to provide a seed dressing and spreading method using a biostimulant and flutriafol in combination, so as to solve at least one of the technical problems existing in the prior art.

[0005] In order to solve the above-mentioned technical problems, the technical solution adopted by the present invention is: a method for seed dressing and spreading using a biostimulant and flutriafol in combination, first, the biostimulant and flutriafol are prepared into a mixture according to the mass ratio of active ingredients of 1-50:1-10, and then the mixture is used for seed dressing or spreading; the seed dressing refers to preparing the mixture of the biostimulant and flutriafol into a seed dressing agent or other dosage forms suitable for seed dressing, and then applying the mixture as a seed dressing; the spreading refers to preparing the mixture of the biostimulant and flutriafol into granules or other dosage forms suitable for mixing with fertilizers and soil for spreading, and then spreading it.

[0006] The present invention does not limit the specific components of the aforementioned biostimulants. As long as the biostimulants and flutriafol are mixed to achieve a combined effect of improving the prevention and control of crop diseases, it is within the scope of the present invention. At the same time, the present invention does not limit the auxiliary components and dosage forms of the aforementioned mixture to make a specific preparation. As long as it can be made into a dosage form suitable for seed dressing or broadcasting, it is within the scope of the present invention.

[0007] The following is only a technical solution for further optimization and is not intended to limit the aforementioned independent solutions.

[0008] Furthermore, the spreading includes making the mixture of biostimulant and flutriafol into granules, mixing it thoroughly with wheat seeds and then sowing it in rows; or mixing it thoroughly with corn base fertilizer and topdressing fertilizer and then sowing it in holes; or mixing it thoroughly with rice topdressing fertilizer and then sowing it; or mixing it thoroughly with peanut base fertilizer and then sowing it in rows, holes or sowing it.

[0009] Furthermore, the seed dressing and spreading method is used to prevent and control wheat stem base rot, sheath blight, powdery mildew, rust, and fusarium head blight; or to prevent and control corn stem base rot, sheath blight, rust, large spot disease, and small spot disease; or to prevent and control rice sheath blight and rice blast; or to prevent and control peanut stem base rot and brown spot disease.

[0010] Furthermore, the biostimulant includes any one of humic acid, fulvic acid, crude protein, amino acid, alginate, chitosan, and amino oligosaccharide.

[0011] Furthermore, the mixture is made of humic acid and flutriafol in an active ingredient mass ratio of 1-50:1-5; or made of amino acid and flutriafol in an active ingredient mass ratio of 1-5:1-5; or made of alginic acid and flutriafol in an active ingredient mass ratio of 1-10:1-5; or made of amino oligosaccharides and flutriafol in an active ingredient mass ratio of 1-20:1-10.

[0012] Furthermore, the mixture is made of humic acid and flutriafol in an active ingredient mass ratio of 20-30:1; or made of amino acid and flutriafol in an active ingredient mass ratio of 1-5:1; or made of alginic acid and flutriafol in an active ingredient mass ratio of 1-5:1; or made of amino oligosaccharides and flutriafol in an active ingredient mass ratio of 1:1 or 1:10.

[0013] Compared with the prior art, the present invention can achieve the ultimate goal of jointly improving the effect of preventing and controlling crop diseases, reducing the amount of pesticides used, extending the effective period, delaying the development of drug resistance, reducing the cost of disease prevention and control, and reducing the damage of pesticides to the environment. DETAILED DESCRIPTION

[0014] The original intention of developing the present invention is that the inventor accidentally discovered that there is a significant difference in the prevention and control effect between the granules containing biostimulants and those not containing biostimulants when the granules were used for spreading 1% fluazifop granules to prevent and control rice sheath blight. In order to further verify this, the following experiment was specially designed.

[0015] 1. To verify whether the biostimulant mixed with flutriafol has a synergistic effect when applied by broadcasting to control rice sheath blight.

[0016] 1.1 Sample formulation

[0017] Serial number Flutriafol content Other ingredients and content Sample 1 1％ Kaolin 99% Sample 2 1％ Humic acid 0.1%, kaolin to make up 100% Sample 3 1％ Humic acid 1%, kaolin to make up 100% Sample 4 1％ Humic acid 10%, kaolin clay 100% Sample 5 1％ Humic acid 20%, kaolin to make up 100% Sample 6 1％ Humic acid 30%, kaolin to make up 100% Sample 7 1％ Humic acid 50%, kaolin clay 100% Sample 8 1％ Humic acid 60%, kaolin clay 100% Sample 9 1％ Amino acid 0.1%, kaolin supplement 100% Sample 10 1％ Amino Acid 1%, Kaolin Complement 100% Sample 11 1％ Amino acid 5%, kaolin supplement 100% Sample 12 1％ Amino Acid 10%, Kaolin Complement 100% Sample 13 1％ Amino acid 20%, kaolin supplement 100% Sample 14 1％ Aminooligosaccharides 0.1%, Kaolin 100% Sample 15 1％ Aminooligosaccharides 1%, Kaolin Complement 100% Sample 16 1％ Oligosaccharides 5%, Kaolin 100% Sample 17 1％ Oligosaccharides 10%, Kaolin 100% Sample 18 1％ Aminooligosaccharides 20%, Kaolin 100% Sample 19 1％ Oligosaccharides 30%, Kaolin 100% Sample 20 1％ Oligosaccharides 50%, Kaolin 100% Sample 21 1％ Alginic acid 0.1%, kaolin to make up 100% Sample 22 1％ Alginic acid 1%, kaolin to make up 100% Sample 23 1％ Alginic acid 5%, kaolin to make up 100% Sample 24 1％ Alginic acid 10%, kaolin to make up 100% Sample 25 1％ Alginic acid 20%, kaolin to make up 100% Sample 26 0％ Humic acid 20%, kaolin to make up 100% Sample 27 0％ Amino acid 5%, kaolin supplement 100% Sample 28 0％ Oligosaccharides 10%, Kaolin 100% Sample 29 0％ Alginic acid 5%, kaolin to make up 100%

[0018] Note: Humic acid of the present invention was purchased from humic acid powder type 1 (humic acid content 55%) from Shanxi Xinjinxiang Agricultural Technology Co., Ltd. Amino acids were purchased from amino acid powder (amino acid content 25%) from Henan Kangfen Biotechnology Co., Ltd. Amino oligosaccharides were purchased from Guangzhou Guanshi Biotechnology Co., Ltd. Alginate was purchased from seaweed powder (alginate content 22.8%) from Obeisi Agricultural Technology Development (Jiangsu) Co., Ltd. The contents of biostimulants in the above table have been converted.

[0019] 1.2 Test method (pot method)

[0020] The pot test was carried out in the applicant's light greenhouse. The cultivated rice seedlings were transplanted into plastic pots, and the cultivated rice sheath blight bacteria cake was inoculated into the leaf sheath at the base of the rice using an inoculation loop. The temperature in the greenhouse was maintained at 29-32°C during the day and 21-24°C at night, and the humidity was above 80%, so that the rice in each pot was evenly diseased. Seven days after inoculation, 300 pots of grade 1 diseased plants with uniform disease were selected, and 10 pots of rice were treated with each sample, and 10 pots of blank controls were set up. Each sample was sprayed at a dosage of 3kg / mu (converted to the area of ​​the plastic pot, and the height of the water in the pot was kept at about 3cm). After 10 and 20 days, the occurrence of rice sheath blight in each treatment was investigated and recorded.

[0021] The disease classification standards, disease index and prevention efficacy calculation are as follows:

[0022] The grading standards for rice sheath blight are as follows: Grade 0 - no symptoms on the leaf sheaths and leaves of the plant; Grade 1 - a few sporadic lesions at the base of the rice plant; Grade 3 - lesions extend to the third leaf from the bottom; Grade 5 - lesions extend to the second leaf from the bottom; Grade 7 - lesions extend to the first leaf from the bottom; Grade 9 - lesions extend to the sword leaf or the entire plant dies.

[0023] Calculation method: disease index (%) = ∑ (number of diseased plants at each level × representative value at each level) / (total number of plants surveyed × highest representative value) × 100; control effect (%) = (blank control disease index - drug treatment disease index) / blank control disease index × 100.

[0024] 1.3 Experimental results

[0025] The pot test results of each sample on rice sheath blight are shown in the table below. It can be seen from the table that flutriafol and humic acid 1:1-1:50, flutriafol and amino acid 1:1-1:5, flutriafol and amino oligosaccharides 10:1-1:20, flutriafol and alginate 1:1-1:10 all showed obvious synergistic effects, while other formulations did not show synergistic effects, and were even worse than the effects of a single agent.

[0026]

[0027] 2. To verify whether the biostimulant mixed with fluazifop has a synergistic effect when sprayed to control rice sheath blight.

[0028] 2.1 Sample formulation

[0029] Serial number Flutriafol content Other ingredients and content Sample 1 1％ Bentonite clay make up 100% Sample 2 1％ Humic acid 20%, bentonite to make up 100% Sample 3 1％ Amino acid 5%, bentonite supplement 100% Sample 4 1％ Oligosaccharides 10%, Bentonite 100% Sample 5 1％ Alginic acid 5%, bentonite to make up 100% Sample 6 0％ Humic acid 20%, bentonite to make up 100% Sample 7 0％ Amino acid 5%, bentonite supplement 100% Sample 8 0％ Oligosaccharides 10%, Bentonite 100% Sample 9 0％ Alginic acid 5%, bentonite to make up 100%

[0030] 2.2 Test method (pot method)

[0031] The difference from 1.2 is the application method: each sample is diluted with 100 times water and sprayed on rice plants. The rest is the same as the test method described in 1.2.

[0032] 2.3 Experimental results

[0033] The pot test results of each sample spraying on rice sheath blight are shown in the following table. It can be seen from the following table that only the mixture of flutriafol and amino oligosaccharide at a ratio of 1:10 showed obvious synergistic effect, while other formulations did not show synergistic effect. Compared with experiment 1, whether other biostimulants can only promote systemic synergy still needs to be demonstrated by more subsequent experiments, and the present invention will not be further studied for the time being.

[0034]

[0035] 3. To verify whether the mixture of biostimulants and flutriafol used as seed dressing has a synergistic effect in controlling wheat powdery mildew.

[0036] 3.1 Sample formulation

[0037] Serial number Flutriafol content Other ingredients and content Sample 1 1％ Kaolin clay supplement 100% Sample 2 1％ Humic acid 20%, kaolin to make up 100% Sample 3 1％ Amino acid 5%, kaolin supplement 100% Sample 4 1％ Oligosaccharides 10%, Kaolin 100% Sample 5 1％ Alginic acid 5%, kaolin to make up 100% Sample 6 0％ Humic acid 20%, kaolin to make up 100% Sample 7 0％ Amino acid 5%, kaolin supplement 100% Sample 8 0％ Oligosaccharides 10%, Kaolin 100% Sample 9 0％ Alginic acid 5%, kaolin to make up 100%

[0038] 3.2 Test method (pot method)

[0039] The above 9 samples were mixed with wheat seeds at a dosage of 60g per kg of wheat seeds, and then sown into potted plants. Each treatment had 10 pots, 3 wheat seedlings per pot, and 10 pots were set as blank controls. After the wheat seedlings grew 3 leaves, the fresh spores of powdery mildew fungi were evenly shaken off on each pot of wheat plants. After 10 days, the 10 pots of blank controls had stable disease. At this time, the control effect of each treatment on wheat powdery mildew was investigated. According to the wheat leaf powdery mildew grading standard, the disease index of the whole plant was investigated, and the efficacy was evaluated with the blank control. Disease grading standard: 0-leaf no lesions; 1-lesion area accounts for less than 5% of the total leaf area; 3-lesion area accounts for 6-10% of the total leaf area; 5-lesion area accounts for 11-25% of the total leaf area; 7-lesion area accounts for 26-50% of the total leaf area; 9-lesion area accounts for more than 50% of the total leaf area. Control effect (%) = (blank control disease index - drug treatment disease index) / blank control disease index × 100.

[0040] 3.3 Experimental results

[0041] The results of the pot test of various samples on wheat powdery mildew are shown in the following table.

[0042] As can be seen from the table below, humic acid, amino acids, amino oligosaccharides and alginate are mixed with trifloxystrobin in specific proportions of 20:1, 5:1, 10:1 and 5:1 and applied as seed dressing, which can also play a good synergistic role in the prevention and control of wheat powdery mildew.

[0043]

[0044] 4. Field experiment on prevention and control of wheat diseases

[0045] Test group: 1% flutriafol, 1% amino oligosaccharide, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, 10% kaolin. The raw materials are mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extrusion granulator, dried and sieved to obtain 2% flutriafol·amino oligosaccharide granules.

[0046] Control group 1: 1% flutriafol, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, and kaolin is added to 100%. The raw materials are mixed evenly, crushed, moistened with water, and stirred evenly, then granulated with a screw extruder granulator, dried and sieved to obtain 1% flutriafol granules.

[0047] Control group 2: 1% amino oligosaccharide, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, kaolin to 100%. The raw materials were mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extruder, dried and sieved to obtain 1% amino oligosaccharide granules.

[0048] Mix the three granule samples mentioned above with wheat seeds at a dosage of 2 kg per mu and then sow them in rows. Set up a blank control without any agent seed treatment. Other management levels remain the same. Track the occurrence of each disease and keep statistical records.

[0049] The control effect of each treatment on wheat diseases:

[0050] deal with powdery mildew Sheath blight Stem rot Rust Fusarium head blight Experimental Group 83.5％ 81.3％ 75.8％ 85.5％ 74.9％ Control group 1 62.6％ 60.8％ 58.5％ 65.3％ 68.4％ Control group 2 20.0％ 18.5％ 16.4％ 22.3％ 19.7％

[0051] 5. Field test on prevention and control of corn diseases

[0052] Test group: 1% flutriafol, 20% humic acid, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, 100% kaolin. The raw materials were mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extruder, dried and sieved to obtain 21% flutriafol·humic acid granules.

[0053] Control group 1: 1% flutriafol, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, and kaolin is added to 100%. The raw materials are mixed evenly, crushed, moistened with water, and stirred evenly, then granulated with a screw extruder granulator, dried and sieved to obtain 1% flutriafol granules.

[0054] Control group 2: 20% humic acid, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, kaolin to 100%. The raw materials were mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extruder, dried and sieved to obtain 20% humic acid granules.

[0055] The three granule samples were mixed with corn base fertilizer at a dosage of 2 kg per mu and then sown in holes; a blank control was set up without any pesticide treatment. Other management levels remained the same. Track the occurrence of each disease and keep statistical records.

[0056] The control effect of each treatment on corn diseases:

[0057]

[0058]

[0059] 6. Field experiment on controlling peanut diseases

[0060] Test group: 1% flutriafol, 1% alginic acid, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, kaolin to 100%. The raw materials were mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extruder, dried and sieved to obtain 2% flutriafol·alginic acid granules.

[0061] Control group 1: 1% flutriafol, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, and kaolin is added to 100%. The raw materials are mixed evenly, crushed, moistened with water, and stirred evenly, then granulated with a screw extruder granulator, dried and sieved to obtain 1% flutriafol granules.

[0062] Control group 2: 1% alginate, 5% fatty amide N-methyl sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% calcium dodecylbenzene sulfonate, 3% polyvinyl alcohol, 10% bentonite, kaolin to 100%. The raw materials were mixed evenly, crushed, moistened with water and stirred evenly, then granulated with a screw extruder, dried and sieved to obtain 1% alginate granules.

[0063] Mix the three granule samples mentioned above with peanut base fertilizer at a dosage of 2 kg per mu and spread them. Set up a blank control without any pesticide treatment. Other management levels remain the same. Track the occurrence of each disease and keep statistical records.

[0064] The control effect of each treatment on peanut diseases:

[0065] deal with Stem rot Brown spot Experimental Group 85.2％ 78.8％ Control group 1 52.9％ 55.3％ Control group 2 23.6％ 16.1％

Claims

1. A seed dressing and spreading method using a biostimulant and flutriafol in combination, characterized in that: First, a biostimulant and flutriafol are made into a mixture, and then the mixture is used for seed dressing or broadcasting; the seed dressing refers to making the mixture of the biostimulant and flutriafol into a seed dressing agent or other dosage forms suitable for seed dressing, and then applying the mixture for seed dressing; the broadcasting refers to making the mixture of the biostimulant and flutriafol into a granule or other dosage forms suitable for mixing with fertilizers and soil for broadcasting, and then broadcasting; The mixture is prepared from humic acid and flutriafol in an active ingredient mass ratio of 20-30:1; or from amino acid and flutriafol in an active ingredient mass ratio of 1-5:1; or from alginic acid and flutriafol in an active ingredient mass ratio of 1-5:1; or from amino oligosaccharides and flutriafol in an active ingredient mass ratio of 1:

1.

2. The seed dressing and spreading method of the combined use of biostimulants and flutriafol according to claim 1, characterized in that: The spreading includes making a mixture of biostimulant and flutriafol into granules, mixing it with wheat seeds and then sowing it in rows; or mixing it with corn base fertilizer and topdressing fertilizer and then sowing it in holes; or mixing it with rice topdressing fertilizer and then sowing it; or mixing it with peanut base fertilizer and then sowing it in rows, holes or sowing it.

3. The seed dressing and spreading method of the combined use of biostimulants and flutriafol according to claim 1, characterized in that: The seed dressing and spreading method is used for preventing and controlling wheat stem base rot, sheath blight, powdery mildew, rust, and fusarium head blight; or for preventing and controlling corn stem base rot, sheath blight, rust, large spot disease, and small spot disease; or for preventing and controlling rice sheath blight and rice blast; or for preventing and controlling peanut stem base rot and brown spot disease.

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