An insect toxicity determination device and method

Abstract

The present invention discloses an insect toxicity determination device and method, which relates to the technical field of insect toxicity determination. The device comprises a leaf preservation and drying cover, a spray leaf immersion chamber and a drug storage bin; the leaf preservation and drying cover is connected to the spray leaf immersion chamber via a matching piece directly above the spray leaf immersion chamber; the drug storage bin is connected to the spray leaf immersion chamber via a matching piece directly below the spray leaf immersion chamber. The present invention dries the immersed leaves through the leaf preservation and drying cover, avoids the disadvantage of having to carry a large amount of filter paper when working in the field by using the leaf immersion method, and improves the efficiency of insect toxicity determination.

Description

Technical Field

[0001] The present invention relates to the technical field of insect toxicity determination equipment, and in particular to an insect toxicity determination device and method. Background Art

[0002] Pest control includes chemical control, biological control and agricultural control. Chemical control is quick and has low cost, so it has a wide range of applications. Before chemical control, it is necessary to test the toxicity of insects to determine the concentration of pesticides to be used.

[0003] The leaf dipping method can be used to determine the toxicity of Lepidoptera insects such as the diamondback moth, beet armyworm, fall armyworm, and tomato leafminer. According to relevant regulations, when using the leaf dipping method to determine the toxicity of insects in the field, it is necessary to pre-prepare a large amount of insecticides with equal or equal concentrations and carry them in multiple beakers; it is also necessary to carry a large amount of filter paper to dry the leaves after dipping, which makes it inconvenient for the staff to carry and has low work efficiency. Therefore, how to propose an insect toxicity determination device and method to achieve efficient and quick application of the leaf dipping method to complete the toxicity determination of insects, improve the work efficiency of scientific researchers and reduce the workload of scientific researchers has become a difficult problem that needs to be solved urgently by technical personnel in this field. Summary of the invention

[0004] The object of the present invention is to provide an insect toxicity determination device and method, so as to at least partially solve the problem that the existing insect toxicity determination device is inconvenient to carry and has low determination efficiency.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] An insect toxicity determination device comprises a leaf preservation and drying cover, a spray leaf dipping chamber and a drug storage bin;

[0007] The leaf fresh-keeping and drying cover and the medicine storage bin are respectively connected to the upper and lower sides of the spray leaf immersion chamber.

[0008] The leaf preservation and drying cover and the medicine storage bin are connected to the upper and lower sides of the spray leaf immersion chamber through thread matching, a clamping structure or a buckle.

[0009] The leaf fresh-keeping and drying cover comprises a fan, a leaf moisturizing tube, a hollow cylindrical body and a top cover plate;

[0010] The fan is hingedly connected to the upper side of the hollow cylindrical body through a fan hinge;

[0011] The top cover is connected to the other upper side of the hollow cylinder body through a top cover hinge;

[0012] A breathable gauze is fixed to the hollow portion of the hollow cylindrical body;

[0013] The blade moisturizing tube is fixed to the main body of the hollow cylindrical body, the blade moisturizing hole is arranged above the main body of the hollow cylindrical body corresponding to the blade moisturizing tube, and the blade moisturizing hole is connected to the blade moisturizing tube;

[0014] The bottom of the hollow cylindrical body is connected to the top of the spray leaf immersion chamber.

[0015] The spray leaf-immersing chamber comprises a bin wall, a bin wall cover, a reagent reflux device, a timing controller, an electric spray pipe and a bottom gauze;

[0016] A window portion is provided on the warehouse wall, and the warehouse wall cover is connected to a side edge of the window portion through a warehouse wall cover hinge;

[0017] The timing controller is fixed on the outer wall of the warehouse;

[0018] The electric spray pipe is arranged inside the warehouse wall; the electric spray pipe is connected to the timing controller through a wire;

[0019] The bottom gauze is fixed to the hollow portion of the bottom of the warehouse wall; the medicine reflux device is also fixed to the hollow portion of the bottom of the warehouse wall; the medicine reflux device is located below the bottom gauze.

[0020] The window portion of the warehouse wall is also fixed with a warehouse wall gauze.

[0021] The warehouse wall is made of transparent plastic material.

[0022] The medicine reflux device is designed to be funnel-shaped.

[0023] There are multiple electric spray pipes, and the multiple electric spray pipes are evenly distributed in a circle inside the warehouse wall.

[0024] A method applied to the above insect toxicity determination device comprises the following steps:

[0025] S1: Adding the agent, connecting the drug storage bin containing the agent of preset concentration to the bottom of the spray leaf immersion chamber;

[0026] S2: Positioning the leaves, fixing the leaves to be tested on the leaf preservation and drying cover;

[0027] S3: connecting a leaf preservation and drying cover, wherein the leaf preservation and drying cover on which the leaves to be tested are fixed is connected above the spray leaf immersion chamber;

[0028] S4: spraying of the agent, the spray leaf immersion chamber converts the liquid agent in the drug storage bin into mist through the electric spray pipe, and sprays it onto the leaves to be tested fixed on the leaf preservation and drying cover;

[0029] S5: the leaves are dried, the spraying chamber stops spraying, and after the leaves to be tested fixed on the leaf preservation and drying cover are dried, the leaf preservation and drying cover is removed from the spraying chamber;

[0030] S6: placing insects to be tested into the spray leaf immersion chamber, and reconnecting the leaf fresh-keeping and drying cover with the dried leaves fixed thereon to the top of the spray leaf immersion chamber;

[0031] S7: Observation and recording, removing the drug storage bin from the spray leaf immersion chamber, and observing and recording the number of dead and alive insects to be tested after a preset time a;

[0032] S8: modifying the concentration of the drug, modifying the concentration of the drug in the drug storage bin;

[0033] S9: repeating steps S1 to S8, and calculating the use concentration of the test agent according to the multiple recorded numbers of dead and alive insects.

[0034] Compared with the prior art, the beneficial technical effects of the present invention are:

[0035] The present invention provides an insect toxicity determination device and method, wherein the device includes a leaf preservation and drying cover, a spray leaf immersion chamber, and a drug storage bin; the leaf preservation and drying cover is connected to the spray leaf immersion chamber via a connector directly above the spray leaf immersion chamber; the drug storage bin is connected to the spray leaf immersion chamber via a connector directly below the spray leaf immersion chamber. Compared with the traditional device that requires a large amount of filter paper to dry the leaf immersion leaves, the present invention only requires a leaf preservation and drying cover to complete the leaf drying work, avoiding the disadvantage of carrying a large amount of filter paper when conducting leaf immersion experiments in the field. This makes the device of the present invention easy to carry, thereby improving the determination efficiency of the device.

[0036] A fan is arranged on the leaf preservation and drying cover of the device, and the fan can be used to speed up the drying of the immersed leaf blades, thereby further improving the measuring efficiency of the device.

[0037] In addition, the device is provided with a pesticide reflux device to facilitate the recycling of the pesticide and save pesticides.

[0038] During the experimental measurement process, the experimenters do not directly contact the drugs, thus ensuring the safety of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The present invention will be further described below in conjunction with the accompanying drawings.

[0040] Figure 1 This is a schematic diagram of the overall structure of the insect toxicity determination device of the present invention;

[0041] Figure 2 This is a schematic diagram of the structure of the leaf preservation and drying cover of the present invention;

[0042] Figure 3 It is a structural schematic diagram of the spray leaf immersion chamber of the present invention;

[0043] Figure 4 It is a structural schematic diagram of the medicine storage bin of the present invention.

[0044] Explanation of the reference numerals: 1. Leaf preservation and drying cover; 1.1. Fan; 1.2. Fan hinge; 1.3. Leaf moisturizing hole; 1.4. Leaf moisturizing tube; 1.5. Hollow cylinder body; 1.6. Top cover; 1.7. Top cover hinge; 1.8 Breathable gauze; 2. Spray leaf immersion chamber; 2.1. Silo wall; 2.2 Silo wall gauze; 2.3. Silo wall cover hinge; 2.4. Silo wall cover; 2.5. Drug reflux device; 2.6. Timing controller; 2.7. Wire; 2.8. Electric spray tube; 2.9. Bottom gauze; 3. Drug storage bin. DETAILED DESCRIPTION

[0045] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0046] The object of the present invention is to provide an insect toxicity determination device and method, so as to solve the problems that the existing insect toxicity determination devices are inconvenient to carry and have low determination efficiency.

[0047] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0048] Example 1

[0049] like Figure 1-4 As shown, this embodiment discloses an insect toxicity determination device, including a leaf preservation and drying cover 1, a spray leaf dipping chamber 2 and a drug storage bin 3;

[0050] The leaf preservation and drying cover 1 and the medicine storage bin 3 are respectively connected to the upper and lower sides of the spray leaf immersion chamber 2.

[0051] When working in the field, the traditional leaf dipping method device is not convenient to carry. The traditional leaf dipping method device mainly uses a beaker and a large amount of filter paper, wherein the beaker is used to hold various concentrations of pesticides, and the filter paper is used to spread the leaves after dipping to assist in the leaf drying operation. The present invention uses the leaf preservation and drying cover 1 to dry the leaves after dipping, thereby avoiding the disadvantage of carrying a large amount of filter paper, and is more convenient to carry than the traditional leaf dipping method device, thereby improving the efficiency of the leaf dipping method determination.

[0052] It should be noted that although the traditional leaf dipping method can also be used for laboratory operations, laboratory operations require the transportation of the insects to be tested, which will reduce the biological activity of the insects to be tested, thereby affecting the accuracy of the experimental results. The insect toxicity determination device provided by the present invention can be conveniently used for field operations, avoiding the disadvantage of affecting the experimental results due to the transportation of the insects to be tested.

[0053] Specifically, the leaf preservation and drying cover 1 and the medicine storage bin 3 are connected to the upper and lower sides of the spray leaf immersion chamber 2 by threaded matching, a clamping structure or a buckle, so as to facilitate the connection and separation operation of the spray leaf immersion chamber 2 with the leaf preservation and drying cover 1 and the medicine storage bin 3 respectively.

[0054] like Figure 2 As shown, this embodiment also discloses a specific structure of a leaf preservation and drying cover 1, wherein the leaf preservation and drying cover 1 comprises a fan 1.1, a leaf moisturizing tube 1.4, a hollow cylindrical body 1.5 and a top cover plate 1.6;

[0055] The fan 1.1 is connected to the upper side of the hollow cylindrical body 1.5 via a fan hinge 1.2;

[0056] The top cover plate 1.6 is connected to the other upper side of the hollow cylindrical body 1.5 through the top cover plate hinge 1.7;

[0057] A breathable gauze 1.8 is fixed to the hollow portion of the hollow cylindrical body 1.5;

[0058] The blade moisturizing tube 1.4 is fixed to the main body of the hollow cylindrical body 1.5, and the blade moisturizing hole 1.3 is arranged above the main body of the hollow cylindrical body 1.5 corresponding to the blade moisturizing tube 1.4, and the blade moisturizing hole 1.3 is connected to the blade moisturizing tube 1.4;

[0059] The bottom of the hollow cylindrical body (1.5) is connected to the top of the spray leaf immersion chamber (2).

[0060] In the specific implementation, the petiole of the leaf to be tested is inserted into the leaf moisturizing tube 1.4 for fixing. In order to prevent the leaf to be tested from drying out due to lack of water in subsequent operations, the leaf moisturizing tube 1.4 is filled with water through the leaf moisturizing hole 1.3 after the leaf to be tested is fixed.

[0061] In addition, the fan 1.1 is used to speed up the drying of the leaves to be tested after dipping, which can further improve the testing efficiency of the insect toxicity testing device of the present invention. Among them, the breathable gauze 1.8 is used to assist the fan 1.1 in completing the accelerated drying of the leaves to be tested after dipping, and is also used to prevent the insects to be tested from escaping. When the device of the present invention sprays and dips the leaves to be tested, the top cover plate 1.6 participates in closing the spray and dipping chamber 2 to prevent the leakage of the drug mist and improve the safety of the device operation.

[0062] like Figure 3 As shown, this embodiment also discloses a specific structure of a spray leaf immersion chamber 2, wherein the spray leaf immersion chamber 2 includes a bin wall 2.1, a bin wall cover plate 2.4, a reagent reflux device 2.5, a timing controller 2.6, an electric spray pipe 2.8 and a bottom gauze 2.9;

[0063] The warehouse wall 2.1 is provided with a window portion, and the warehouse wall cover 2.4 is connected to one side of the window portion through a warehouse wall cover hinge 2.3;

[0064] The timing controller 2.6 is fixed on the outer wall of the warehouse wall 2.1;

[0065] The electric spray pipe 2.8 is arranged inside the warehouse wall 2.1; the electric spray pipe 2.8 is connected to the timing controller 2.6 through a wire 2.7;

[0066] The bottom gauze 2.9 is fixed to the hollow portion at the bottom of the warehouse wall 2.1; the medicine reflux device 2.5 is also fixed to the hollow portion at the bottom of the warehouse wall; the medicine reflux device 2.5 is located below the bottom gauze 2.9.

[0067] Specifically, the window portion of the warehouse wall 2.1 is also fixed with a warehouse wall gauze 2.2;

[0068] Among them, the gauze 2.2 of the warehouse wall participates in the drying of the leaves to be tested after leaf dipping, and is also used for ventilation of the insects to be tested. The bottom gauze 2.9 participates in the ventilation of the insects to be tested, which is used to prevent the insects to be tested from falling into the medicine storage bin 3 at the bottom, and is also used to assist the spray of the medicine to flow back to the medicine storage bin 3 through the medicine reflux device 2.5. When performing leaf dipping spraying operations, the warehouse wall cover 2.4 participates in closing the spray dipping chamber 2 to improve the safety of the device operation.

[0069] Specifically, in this embodiment, the medicine reflux device 2.5 is designed to be funnel-shaped, but in specific implementation, the medicine reflux device 2.5 can also be designed to be groove-shaped, and the present invention does not make specific limitations on this.

[0070] Specifically, the warehouse wall 2.1 is made of transparent plastic material, and specifically, the warehouse wall 2.1 can be made of polymethyl methacrylate, polystyrene or polycarbonate.

[0071] Specifically, there are multiple electric spray pipes 2.8, which are evenly distributed in a circle inside the warehouse wall 2.1. In this embodiment, there are five electric spray pipes 2.8, four of which are distributed around the spray leaf immersion chamber 2, and one is distributed in the center of the spray leaf immersion chamber 2. The timing controller 2.6 is provided with a stopwatch and an adjustable time button, the adjustable time button is used to accurately set the spray time, and the stopwatch is used to display the spray time. When performing the spraying operation of the agent, the electric spray pipe 2.8 is controlled by the timing controller 2.6 to perform the spraying operation, and the agent spray is evenly sprayed onto the leaves to be tested.

[0072] Specifically, the overall specifications of the insect toxicity determination device are set to a diameter of 10 cm and a height of 15 cm. Among them, the leaf moisturizing tube 1.4 is set to a diameter of 1.5 cm and a height of 5 cm. Among them, the electric spray tube 2.8 is set to 7 cm, and the medicine storage bin is set to a height of 4 cm. The insect toxicity determination device is small and compact and easy to carry. The leaf preservation and drying cover 1 is used to dry the leaves to be tested after dipping, avoiding the disadvantage that the staff carry a large amount of filter paper to assist in drying the leaves, and is convenient for the staff to carry; at the same time, the fan 1.1 is used to accelerate the drying of the leaves to be tested after dipping, thereby improving the determination efficiency of the device when using the dipping method for insect toxicity determination.

[0073] Example 2

[0074] This embodiment also provides a method for applying the insect toxicity determination device provided in Embodiment 1, comprising the following steps:

[0075] S1: Remember to add, connect the medicine storage bin 3 filled with medicine of preset concentration to the bottom of the spray leaf immersion chamber 2;

[0076] Specifically, the medicine storage bin 3 is screwed under the spray leaf immersion chamber 2 through threads.

[0077] S2: Positioning the leaves, fixing the leaves to be tested on the leaf preservation and drying cover 1;

[0078] In the specific implementation, the petiole of the leaf to be tested is inserted into the leaf moisturizing tube 1.4 for fixation; at the same time, water is injected through the leaf moisturizing hole 1.3 to moisturize the leaf to be tested.

[0079] S3: connecting the leaf preservation and drying cover, connecting the leaf preservation and drying cover 1 fixed with the leaves to be tested above the spray leaf immersion chamber 2;

[0080] Specifically, the leaf preservation and drying cover 1 can be screwed onto the top of the spray leaf immersion chamber 2 through a thread.

[0081] S4: spraying of the agent, the spraying and leaf soaking chamber 2 converts the liquid agent in the drug storage bin 3 into mist through the electric spray pipe 2.8, and sprays it onto the leaves to be tested fixed on the leaf preservation and drying cover 1;

[0082] Specifically, the electric spray pipe 2.8 is controlled by the timing controller 2.6 to perform the spraying operation, and the specific spraying time is controlled by the timing controller 2.6. Before the blade to be tested is sprayed, the top cover 1.6 and the bin wall cover 2.4 are closed in advance to prevent the spray of the agent from leaking out and improve the safety of the device operation.

[0083] S5: The leaves are dried, the spraying chamber 2 stops spraying, and after the leaves to be tested fixed on the leaf preservation and drying cover 1 are dried, the leaf preservation and drying cover 1 is removed from the spraying chamber 2;

[0084] In this step, the operator can accelerate the drying of the blade to be tested by means of the fan 1.1.

[0085] S6: placing insects to be tested into the spray leaf immersion chamber 2, and reconnecting the leaf preservation and drying cover 1 fixed with dried leaves to the top of the spray leaf immersion chamber 2;

[0086] S7: Observation and recording: remove the drug storage bin 3 from the spray leaf immersion chamber 2, and observe and record the number of dead and alive insects after a preset time a;

[0087] Specifically, the preset time a is set to 48 hours.

[0088] S8: changing the concentration of the drug, modifying the concentration of the drug in the drug storage bin 3;

[0089] S9: repeating steps S1 to S8, and calculating the use concentration of the test agent according to the multiple recorded numbers of dead and alive insects.

[0090] Specifically, by modifying the concentration of the drug in the drug storage bin 3 six times and recording the number of dead and alive insects to be tested six times, the concentration of the drug to be tested can be calculated more accurately.

[0091] The tomato seedling leaves are used as the test leaves, the 3rd instar larvae of tomato leafminers are used as the test insects, 25g / L deltamethrin emulsifiable concentrate is used as the test agent, and water is diluted to 0.0375g / L, 0.375g / L, 0.75g / L, 1.875g / L, 3.75g / L5 concentrations, and clean water is used as the control. Each concentration of deltamethrin emulsifiable concentrate is tested 4 times with the device of the present invention and the traditional device. Each test uses 15 3rd instar larvae of tomato leafminers, and the number of deaths is observed and counted after 48 hours. The time required for the operation of the device of the present invention and the traditional device is counted during the test. The following results are obtained:

[0092] Table 1 Comparative test of the present invention and the traditional leaf dipping device using deltamethrin as insecticide

[0093]

[0094] Among them, LC 50 Lethal Concentration 50 The abbreviation of , refers to the concentration of poison that kills half of the test animals in the animal acute toxicity test. The chi-square value is used to measure the degree of difference between the observed value and the expected value. The larger the chi-square value, the greater the difference between the observed value and the expected value, and the more significant the difference between the two. Table 1 shows that after 48 hours, the LC of the third instar larvae of the tomato leafminer measured by the device of the present invention is 50 The LC value of the second-instar larvae of tomato leafminer measured by the traditional leaf dipping method was 3.796 g / L. 50 The time required to complete all operations is 10.283 min for the device of the present invention, which is significantly lower than the 17.450 min of the conventional leaf dipping device; in a single repeated experiment, the insecticide dosage required for the device of the present invention is 30 ml, which is also significantly lower than the 80 ml of the conventional leaf dipping device.

[0095] The results show that the device of the present invention has a shorter operating time, is simpler, requires less pesticide than the traditional device, and produces the same results as the traditional device, and is capable of determining insect toxicity by the leaf dipping method.

[0096] Under laboratory conditions, the leaf dipping method was used with corn seedling leaves as the test leaves and the third-instar larvae of the fall armyworm as the test insects. Gradient concentrations of avermectin benzoate, spinetoram, and chlorantraniliprole were used to determine the insect toxicity of the third-instar larvae of the fall armyworm, and the following results were obtained:

[0097] Table 2 The results of the toxicity test of the three insecticides of the device of the present invention on the third instar larvae of the fall armyworm

[0098]

[0099] The test results showed that the third-instar larvae of the fall armyworm were most sensitive to avermectin benzoate, and LC 50 is 0.056mg / L; it is least sensitive to chlorantraniliprole, and its LC 50 0.315 mg / L; moderate sensitivity to spinetoram, LC 50 The sensitivity of the third instar larvae of fall armyworm to different pesticides is from high to low: avermectin benzoate, spinetoram, and chlorantraniliprole.

[0100] Under laboratory conditions, the leaf dipping method was used to test tomato seedling leaves and the 3rd instar larvae of tomato leafminer as the test insects. Gradient concentrations of avermectin benzoate, spinetoram, and chlorantraniliprole were used to determine the insect toxicity of 3rd instar larvae of tomato leafminer. The following results were obtained:

[0101] Table 3 The results of the toxicity test of the three insecticides of the present invention on the 3rd instar larvae of the tomato leafminer

[0102]

[0103] The results showed that the third-instar larvae of tomato leafminer were most sensitive to avermectin benzoate. 50 is 0.059 mg / L; the sensitivity to ethyl spinetoram is the lowest, and its LC 50 is 1.102 mg / L; the sensitivity to chlorantraniliprole is moderate, LC 50 The sensitivity of the third-instar larvae of tomato leafminer to different pesticides is from high to low: avermectin benzoate, chlorantraniliprole, and spinetoram. The experimental results show that this device is capable of determining the toxicity of insects.

[0104] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0105] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. An insect toxicity determination device, characterized in that: It comprises a leaf preservation and drying cover (1), a spray leaf immersion chamber (2) and a medicine storage bin (3), wherein the leaf preservation and drying cover (1) and the medicine storage bin (3) are respectively connected to the upper and lower sides of the spray leaf immersion chamber (2); The leaf preservation and drying cover (1) comprises a fan (1.1), a leaf moisturizing tube (1.4), a hollow cylindrical body (1.5) and a top cover plate (1.6); The fan (1.1) is connected to an upper side of the hollow cylindrical body (1.5) via a fan hinge (1.2); The top cover plate (1.6) is connected to the other upper side of the hollow cylindrical body (1.5) via a top cover plate hinge (1.7); A breathable gauze (1.8) is fixed to the hollow portion of the hollow cylindrical body (1.5); The blade moisturizing tube (1.4) is fixed to the main body of the hollow cylindrical body (1.5); the blade moisturizing hole (1.3) is arranged above the main body of the hollow cylindrical body (1.5) corresponding to the blade moisturizing tube (1.4); and the blade moisturizing hole (1.3) is connected to the blade moisturizing tube (1.4); The bottom of the hollow cylindrical body (1.5) is connected to the top of the spray leaf immersion chamber (2); The spray leaf-immersion chamber (2) comprises a chamber wall (2.1), a chamber wall cover plate (2.4), a medicine reflux device (2.5), a timing controller (2.6), an electric spray pipe (2.8) and a bottom gauze (2.9); A window portion is provided on the warehouse wall cover plate (2.4); the warehouse wall cover plate (2.4) is connected to a side edge of the window portion via a warehouse wall cover plate hinge (2.3); The timing controller (2.6) is fixed on the outer wall of the warehouse wall (2.1); The electric spray pipe (2.8) is arranged inside the warehouse wall (2.1); the electric spray pipe (2.8) is connected to the timing controller (2.6) via a wire (2.7); The bottom gauze (2.9) is fixed to the hollow portion at the bottom of the warehouse wall (2.1); the medicine reflux device (2.5) is also fixed to the hollow portion at the bottom of the warehouse wall (2.1); the medicine reflux device (2.5) is located below the bottom gauze (2.9).

2. The insect toxicity measuring device according to claim 1, characterized in that: The leaf preservation and drying cover (1) and the medicine storage bin (3) are connected to the upper and lower sides of the spray leaf immersion chamber (2) through threaded matching, a snap-fit ​​structure or a buckle.

3. The insect toxicity measuring device according to claim 1, characterized in that: A warehouse wall gauze (2.2) is also fixed to the window portion of the warehouse wall (2.1).

4. The insect toxicity measuring device according to claim 1, characterized in that: The warehouse wall (2.1) is made of transparent plastic material.

5. The insect toxicity measuring device according to claim 1, characterized in that: The medicine reflux device (2.5) is designed to be funnel-shaped.

6. The insect toxicity measuring device according to claim 1, characterized in that: There are a plurality of electric spray pipes (2.8), and the plurality of electric spray pipes (2.8) are evenly distributed in a circle inside the bin wall (2.1).

7. A method for applying the insect toxicity determination device according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1: adding a medicine, connecting a medicine storage bin (3) containing a medicine of a preset concentration to the bottom of the spray leaf immersion chamber (2); S2: Positioning the leaves, fixing the leaves to be tested on the leaf preservation and drying cover (1); S3: connecting the leaf preservation and drying cover, connecting the leaf preservation and drying cover (1) on which the leaves to be tested are fixed to the top of the spray leaf immersion chamber (2); S4: spraying of the agent, the spray leaf immersion chamber (2) converts the liquid agent in the agent storage bin (3) into mist through the electric spray pipe (2.8), and sprays it onto the leaves to be tested fixed on the leaf preservation and drying cover (1); S5: the leaves are dried, the spraying of the leaf-immersing chamber (2) is stopped, and after the leaves to be tested fixed on the leaf-keeping and drying cover (1) are dried, the leaf-keeping and drying cover (1) is removed from the spraying and drying chamber (2); S6: placing insects to be tested into the spray leaf immersion chamber (2), and reconnecting the leaf preservation and drying cover (1) fixed with dried leaves to the top of the spray leaf immersion chamber (2); S7: Observation and recording: removing the drug storage bin (3) from the spray leaf immersion chamber (2), and observing and recording the number of dead and alive insects after a preset time a; S8: changing the concentration of the drug, modifying the concentration of the drug in the drug storage bin (3); S9: repeating steps S1 to S8, and calculating the use concentration of the test agent according to the multiple recorded numbers of dead and alive insects.

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