Anti-hair loss and hair growth essence and preparation method thereof

Abstract

The present application relates to the technical field of daily necessities and specifically discloses an anti-hair loss and growth serum and its preparation method. The anti-hair loss and growth serum of the present application comprises, by weight percentage, the following components: a modified caffeine mixture 0.1-5.0%; a polypeptide mixture 0.1-3.0%; a plant essence 0.1-20.0%; methylpropanediol 0.1-30.0%; PCA zinc 0.1-1.0%; niacinamide 0.05-2.0%; trehalose 0.5-2.0%; betaine 0.1-3.0%; a daily fragrance 0.1-0.6%; PEG-40 hydrogenated castor oil 0.1-1.0%; a preservative 0.2-1.0%; citric acid 0.05-0.2%; sodium citrate 0.05-0.5%; and the balance deionized water. The anti-hair loss and hair growth serum produced with this composition ratio, thanks to its high caffeine content, effectively and consistently enhances its anti-hair loss and hair growth efficacy. Furthermore, the combination of the plant essence and peptide mixture nourishes hair follicles, improves the scalp environment, and promotes hair growth.

Description

Technical Field

[0001] The present application relates to the technical field of daily necessities, and in particular to an anti-hair loss and hair growth essence and a preparation method thereof. Background Art

[0002] Hair loss refers to the phenomenon of hair loss. Physiological hair loss occurs during the normal metabolic process. Normally, the hair that falls out is in the catagen and telogen phases. After the hair falls out, the hair follicles will continue to grow new hair, thus maintaining a dynamic balance in the number of hairs.

[0003] Pathological hair loss refers to abnormal or excessive hair loss, which is mainly divided into neurotic alopecia, endocrine alopecia, nutritional alopecia, etc. Currently, seborrheic alopecia is more common. The main reason is that the level of testosterone (T) in the body is too high, which leads to the degeneration and atrophy of hair follicles, causing the hair roots to enter the resting phase early, thus causing hair loss. In recent years, studies have shown that mixing caffeine with shampoo can effectively prevent hair loss, and caffeine can also stimulate hair growth while increasing the probability of hair follicles in the growth stage. Caffeine has been clinically proven to prevent hair loss by controlling testosterone loss (AGA), specifically by inhibiting the process of type II 5α reductase catalyzing the conversion of testosterone to dihydrotestosterone (DHT).

[0004] Regarding the above technology, the inventor believes that although caffeine is a natural anti-hair loss substance, due to its extremely low water solubility (about 2%) and the precipitation of crystals at low temperatures, the amount of caffeine added to related hair care products is also reduced. Currently, the amount of caffeine used in shampoo is difficult to exceed 0.5%, and the amount of caffeine used in ethanol-based hair growth tonics cannot exceed 0.1%. In addition, the anti-hair loss and hair growth effects produced by the combination of other existing chemical components and caffeine are poor. Summary of the Invention

[0005] In order to improve the anti-hair loss and hair growth efficacy of caffeine in hair care products, the present application provides an anti-hair loss and hair growth essence and a preparation method thereof.

[0006] In the first aspect, the present application provides an anti-hair loss and hair growth essence, which adopts the following technical solution:

[0007] An anti-hair loss and hair growth essence, comprising the following components by weight percentage:

[0008] Modified caffeine mixture 0.1-5.0%;

[0009] Peptide mixture 0.1-3.0%;

[0010] Plant essence 0.1-20.0%;

[0011] Methylpropanediol 0.1-30.0%;

[0012] PCA zinc 0.1-1.0%;

[0013] Niacinamide 0.05-2.0%;

[0014] Trehalose 0.5-2.0%;

[0015] Betaine 0.1-3.0%;

[0016] Daily fragrance 0.1-0.6%;

[0017] PEG-40 hydrogenated castor oil 0.1-1.0%;

[0018] Preservatives 0.2-1.0%;

[0019] Citric acid 0.05-0.2%;

[0020] Sodium citrate 0.05-0.5%;

[0021] The balance was deionized water.

[0022] By adopting the above-mentioned technical solution, a modified caffeine mixture, a peptide mixture, and a plant essence are selected as the main active ingredients for a synergistic combination to enhance the product's anti-hair loss and hair growth effects. Caffeine is hydrogenated to significantly increase its water solubility and improve its storage stability, allowing it to remain dissolved at low temperatures without crystallization. Caffeine, as a functional ingredient, can stimulate hair growth and increase the frequency of hair follicles in the growth phase. The peptide mixture improves the scalp environment, leveraging its targeted effects to more accurately promote the division of hair papilla cells in hair follicles, promoting hair regeneration. It also inhibits and prevents apoptosis of hair follicle cells caused by external factors, maintaining the cells in the active anagen phase for a long time. Plant essences, as natural ingredients, nourish and promote hair regeneration, while also improving hair health. Various plant extracts, as a complex multi-faceted system, can work synergistically with multiple active ingredients to target different hair loss sites to exert their anti-hair loss, anti-hair loss, and hair growth effects. Plant essences also have minimal toxic side effects, are generally non-mutagenic, and are gentle and non-irritating to the scalp, making them suitable for long-term use. Various other additives, such as the moisturizing properties of trehalose and the antioxidant properties of betaine, are used to enhance the overall performance of the essence.

[0023] Preferably, the modified caffeine mixture comprises the following components in percentage by weight:

[0024] Caffeine 9.2-10.0%;

[0025] Niacinamide 9.0-10.0%;

[0026] Dipropylene glycol 4.0-5.0%;

[0027] Glycereth-26 3.8-4.0%;

[0028] 1,2-Hexanediol 1.0-2.0%;

[0029] C12-14 pareth-12 0.7-1.0%;

[0030] Polyglyceryl-10 diisostearate 0.6-1.0%;

[0031] Sodium naphthalenesulfonate 0.6-0.8%;

[0032] C12-13 pareth-9 0.3-0.5%;

[0033] The balance was deionized water.

[0034] Preferably, the method for preparing the modified caffeine mixture comprises the following steps:

[0035] S1. Evenly mix dipropylene glycol, 1,2-hexanediol, and deionized water, raise the temperature to 50-60° C., then add caffeine and nicotinamide, mix, and stir at a constant temperature for 50-90 minutes to prepare a reaction mixture;

[0036] S2. Preheat the initial reaction material in step S1 to 70-78° C., add glycerol polyether-26 and polyglycerol-10 diisostearate in sequence, mix and react for 2-3 hours, cool to 48-54° C., add sodium naphthalenesulfonate and continue stirring until completely dissolved to obtain a reaction intermediate; S3. Add C12-14 paraether-12 and C12-13 paraether-9 to the reaction intermediate in step S2, stir evenly, heat to 100-110° C., keep warm and react for 2-3 hours, and continue stirring until the material is uniform to obtain a modified caffeine mixture.

[0037] By adopting the above technical solution, caffeine, as a xanthine alkaloid compound, on the one hand, controls the loss of testosterone, specifically inhibits the process of testosterone conversion to dihydrotestosterone catalyzed by type II 5α reductase, thereby playing a role in preventing hair loss; on the other hand, it promotes the proliferation of hair keratinocytes to increase the frequency of hair follicles in the growth phase, thereby stimulating hair growth. Caffeine can also activate the capillary microcirculation of the head skin, continuously supplying nutrients to the hair through the blood, thereby promoting rapid hair growth; and caffeine regulates the relevant production factors in the human body, by promoting insulin growth factor (IGF-1) to prolong the growth phase of hair; by inducing transforming growth factor (TGF-β2) to inhibit the expression of hair in the anagen phase, thereby playing a role in preventing hair loss;

[0038] Because caffeine molecules are highly polar, dipropylene glycol and related polyether substances are added to improve their water solubility. On the one hand, they act as hydrogenating agents, providing hydrogen ions within the solvent system, causing hydrogenation reactions on the end groups of the caffeine molecules. This inhibits the formation of hydrogen bonds between the caffeine molecules and the oxygen and hydrogen moieties in water molecules, effectively reducing the recrystallization and condensation of caffeine molecules, making them less soluble in water. On the other hand, dipropylene glycol can also increase the scalp's ability to absorb active ingredients such as caffeine, promoting their penetration into the deeper layers of the skin. Furthermore, by adjusting the viscosity and fluidity of the product essence, it makes it easier to use and apply.

[0039] The addition of other additives enhances the overall performance of the modified mixture. For example, niacinamide, combined with caffeine, protects the scalp from UV damage while improving the skin environment, thereby strengthening the barrier and reducing scalp sensitivity. The addition of 1,2-hexanediol imparts broad-spectrum antimicrobial and preservative activity, particularly against bacteria, yeast, and mold. 1,2-hexanediol, an alkyl vicinal diol, exhibits excellent moisturizing properties, low skin irritation, and excellent safety.

[0040] Preferably, the polypeptide mixture comprises decapeptide-4 and oligopeptide-29, the mass ratio of decapeptide-4 to oligopeptide-29 is 1:(1.10-1.30), and the polypeptide mixture is externally coated with a phospholipid bilayer.

[0041] By adopting the above technical solution, decapeptide-4 and oligopeptide-29 are selected for synergistic cooperation, which has a clear targeting effect and can effectively promote the proliferation of hair papilla cells in hair follicles, achieving hair regeneration. It can also inhibit and prevent the apoptosis of keratinocytes in the hair matrix caused by stress and other factors, delaying their degeneration period, thereby keeping the cells in the active hair growth phase. At the same time, the peptide mixture replenishes nutrients to the scalp and hair roots by promoting angiogenesis in the scalp. By controlling the added mass ratio of decapeptide-4 and oligopeptide-29 and encapsulating them inside a biphospholipid membrane, it can be more easily penetrated into the dermis of the scalp, thereby stably exerting its anti-hair loss, hair growth, and hair cultivation effects.

[0042] Preferably, the plant essence is one or more of Platycladus orientalis leaf extract, Polygonum multiflorum extract and Eclalphia chinensis extract.

[0043] By adopting the above technical solution, Platycladus orientalis leaf extract, Polygonum multiflorum extract, and Eclipta prostrata extract are selected as natural plant essences. Platycladus orientalis leaf extract has a vasodilating effect, which can inhibit excessive sebum secretion on the scalp while improving microcirculation and strengthening hair follicles to achieve the effects of preventing and stopping hair loss and promoting hair growth. Furthermore, Platycladus orientalis leaf extract contains flavonoids and phenolic compounds such as cypress biflavonoids and kaempferol, which have strong antibacterial properties, showing significant antibacterial effects against Candida albicans and Staphylococcus aureus. On the other hand, the alcohols, phenols, and steroids contained in Platycladus orientalis leaf extract also have strong antioxidant properties, scavenging free radicals within pigment cells and thus inhibiting the regeneration of skin pigmentation. Polygonum multiflorum extract, the dried root of the Polygonaceae plant, has been known to blacken hair since ancient times and has long been an important treatment for gray hair and hair loss. It scavenges DPPH free radicals, thus acting as an antioxidant. Decoctions of different processed Polygonum multiflorum products have varying degrees of inhibitory activity against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Shigella sonnei, Salmonella typhi 901, Salmonella paratyphi B, Corynebacterium diphtheriae, beta-hemolytic Streptococcus, and Neisseria catarrhalis. Alcohol extracts of Eclipta prostrata effectively extract active ingredients such as polysaccharides and flavonoids. Applying them to the scalp can effectively promote hair growth and make hair follicles appear rigid and located deep in the subcutaneous tissue layer, thereby reducing hair loss. Furthermore, Eclipta prostrata extract can induce the transition of hair follicles from the resting phase to the anagen phase.

[0044] Preferably, the daily flavor is one or more of rose flavor, green tea flavor, lemon flavor and orange blossom flavor.

[0045] By adopting the above technical solution, various daily-use fragrances are added to the product essence system. While not affecting the efficacy of other anti-hair loss and hair growth components, the product essence can be provided with a unique fragrance, thereby helping users improve their mood, relieve fatigue and anxiety, and eliminate odor on the scalp surface, further making the product essence have a wider audience market.

[0046] Preferably, the preservative is one or more of phenoxyethanol and benzyl alcohol.

[0047] By adopting the above technical solution, phenoxyethanol and benzyl alcohol are selected as preservatives. Phenoxyethanol and benzyl alcohol can act on bacterial cell membranes, increasing their permeability to potassium ions, thereby inhibiting the enzyme activity required for bacterial growth. They have a particularly good antibacterial effect on both Gram-positive and Gram-negative bacteria. At the same time, phenoxyethanol can also cooperate with substances with similar surfactant structures such as glycerol polyether within the system without obvious antagonism. It can affect the interfacial tension of bacterial cell membranes and the osmotic pressure inside bacteria, thereby allowing the preservatives to penetrate more effectively, significantly improving the antiseptic and antibacterial effects of the preservatives and ensuring the quality and safety of the product essence.

[0048] In a second aspect, the present application provides an anti-hair loss and growth essence and a preparation method thereof, comprising the following steps:

[0049] S1. Evenly mix niacinamide, methylpropylene glycol, PCA zinc, trehalose, betaine, citric acid, sodium citrate, and 40-50% of deionized water, heat to 70-75° C., stir and emulsify at a speed of 100-120 r / min, and react for 40-60 min to obtain material A;

[0050] S2. Cooling material A in step S1 to 45-50°C, adding the plant essence, stirring at a speed of 150-200 r / min, and mixing thoroughly; then adding the modified caffeine mixture and mixing for 20-40 minutes to obtain material B;

[0051] S3, cooling the material B in step S2 to 35-40°C, adding the polypeptide mixture, homogenizing and reacting for 30-40 minutes, then sequentially adding the daily fragrance pre-dissolved with PEG-40 hydrogenated castor oil, preservatives and the remaining deionized water, mixing and stirring for 1-2 hours, and degassing to obtain the anti-hair loss and hair growth essence.

[0052] By adopting the above technical solution, niacinamide, methylpropanediol, PCA zinc, trehalose, betaine, citric acid, sodium citrate, and a portion of deionized water are first mixed to form a reaction base liquid. The active reactive groups are activated by emulsification through heating. Various plant ingredients, such as plant essences, are then added. The appropriate temperature and rotation speed are maintained to enhance the reaction efficiency within the system without affecting the activity of the plant extracts. After further cooling, the modified caffeine mixture is added and homogenized to enhance its anti-hair loss and hair growth effects. Subsequently, various additives such as daily fragrances and preservatives are added, and deionized water is supplemented to improve water evaporation caused by the previous heating step. Finally, the temperature is adjusted and the polypeptide mixture is added to complete the functional modification of the product essence. Through homogenization and degassing, a transparent and stable product essence is obtained.

[0053] In summary, this application has the following beneficial effects:

[0054] 1. In this application, by adding a modified caffeine mixture to the essence system, on the one hand, caffeine can prevent hair loss by controlling testosterone loss, specifically by inhibiting the process of testosterone conversion to dihydrotestosterone catalyzed by type II 5α reductase; on the other hand, by promoting the proliferation of hair keratinocytes, the frequency of hair follicles in the growth phase is increased, thereby stimulating hair growth. Caffeine can also activate the capillary microcirculation of the head skin, continuously supplying nutrients to the hair through the blood, thereby promoting rapid hair growth; and caffeine regulates the relevant production factors in the human body, by promoting insulin growth factor (IGF-1) to prolong the growth phase of hair; by inducing transforming growth factor (TGF-β2) to inhibit the expression of hair in the anagen phase, thereby preventing hair loss.

[0055] 2. In this application, dipropylene glycol and related polyether substances are added to improve their water solubility. On the one hand, they act as hydrogenating agents, providing hydrogen ions within the solvent system, causing hydrogenation reactions on the end groups of caffeine molecules. This inhibits the formation of hydrogen bonds between caffeine molecules and the oxygen and hydrogen moieties of water molecules, thereby effectively reducing the phenomenon of caffeine molecules recrystallizing and condensing, making them less soluble in water. On the other hand, dipropylene glycol can also increase the scalp's absorption capacity for active ingredients such as caffeine, promoting their penetration into the deeper layers of the skin. Furthermore, by adjusting the viscosity and fluidity of the product essence, it makes it easier to use and apply.

[0056] 3. In this application, by adding Platycladus orientalis leaf extract, Polygonum multiflorum extract, and Eclipta prostrata extract as natural plant essences, Platycladus orientalis leaf extract has a vasodilatory effect, which can inhibit excessive sebum secretion on the scalp while improving microcirculation and strengthening hair follicles to achieve the effects of preventing, stopping, and promoting hair loss. On the one hand, Platycladus orientalis leaf extract contains flavonoids and phenolic components such as cypress biflavonoids and kaempferol, and has good antibacterial effects, with significant antibacterial effects against Candida albicans and Staphylococcus aureus. On the other hand, the alcohols, phenols, and steroids contained in Platycladus orientalis leaf extract also have strong antioxidant properties, scavenging free radicals within pigment cells and thus inhibiting the regeneration of skin pigmentation. Polygonum multiflorum extract, the dried root of the Polygonaceae plant, has been known to blacken hair since ancient times and has long been an important treatment for gray hair and hair loss. It scavenges DPPH free radicals, thus acting as an antioxidant. Decoctions of different processed Polygonum multiflorum products have varying degrees of inhibitory activity against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Shigella sonnei, Salmonella typhi 901, Salmonella paratyphi B, Corynebacterium diphtheriae, beta-hemolytic Streptococcus, and Neisseria catarrhalis. Alcohol extracts of Eclipta prostrata effectively extract active ingredients such as polysaccharides and flavonoids. Applying them to the scalp can effectively promote hair growth and make hair follicles appear rigid and located deep in the subcutaneous tissue layer, thereby reducing hair loss. Furthermore, Eclipta prostrata extract can induce the transition of hair follicles from the resting phase to the anagen phase.

[0057] 4. In the present application, by selecting decapeptide-4 and oligopeptide-29 for the polypeptide mixture to cooperate with each other, it has a clear targeting effect, can effectively promote the proliferation of hair papilla cells in hair follicles, achieve hair regeneration, and can also inhibit and prevent the apoptosis of keratinocytes in the hair matrix caused by pressure and other factors, delay its degeneration period, and thus keep the cells in the active hair growth period. At the same time, the polypeptide mixture replenishes nutrients to the scalp and hair roots by promoting angiogenesis in the scalp. By controlling the added mass ratio of decapeptide-4 and oligopeptide-29 and wrapping them inside the biphospholipid membrane, it can be made easier to penetrate into the dermis of the scalp, thereby stably exerting its anti-hair loss, hair growth, and hair growth effects. DETAILED DESCRIPTION

[0058] The present application is further described in detail below with reference to the embodiments.

[0059] The raw materials used in the examples and comparative examples can all be obtained commercially.

[0060] Preparation Example

[0061] Preparation of modified caffeine mixture

[0062] The components and weights of Preparation Examples 1-1, 1-2 and 1-3 are shown in Table 1

[0063] Table 1

[0064]

[0065]

[0066] Preparation Example 1-1, a method for preparing a modified caffeine mixture, is prepared by the following method:

[0067] (1) Dipropylene glycol, 1,2-hexanediol, and deionized water were mixed uniformly, heated to 55°C, and then caffeine and nicotinamide were added and stirred at constant temperature for 70 minutes to prepare a reaction mixture;

[0068] (2) preheating the reaction material in step (1) to 74° C., adding glycerol polyether-26 and polyglycerol-10 diisostearate in sequence, mixing and reacting for 2.5 hours, cooling to 51° C., adding sodium naphthalenesulfonate and continuously stirring until completely dissolved, thereby obtaining a reaction intermediate; (3) adding C12-14 paraffin polyether-12 and C12-13 paraffin polyether-9 to the reaction intermediate in step (2), stirring evenly, heating to 105° C., keeping the temperature for reaction for 2.5 hours, and continuously stirring until the material is uniform, thereby obtaining a modified caffeine mixture.

[0069] Preparation Example 1-2, a method for preparing a modified caffeine mixture, is prepared by the following method:

[0070] (1) Dipropylene glycol, 1,2-hexanediol, and deionized water were mixed uniformly, heated to 60°C, and then caffeine and nicotinamide were added and stirred at constant temperature for 90 minutes to prepare a reaction mixture;

[0071] (2) preheating the reaction mixture in step (1) to 78° C., adding glycerol polyether-26 and polyglycerol-10 diisostearate in sequence, mixing and reacting for 3 hours, cooling to 54° C., adding sodium naphthalenesulfonate and stirring continuously until completely dissolved, to obtain a reaction intermediate;

[0072] (3) Add C12-14 paraffinum ether-12 and C12-13 paraffinum ether-9 to the reaction intermediate in step (2), stir evenly, heat to 110° C., keep warm for 3 hours, and continue stirring until the material is uniform to obtain a modified caffeine mixture.

[0073] Preparation Example 1-3, a method for preparing a modified caffeine mixture, is prepared by the following method:

[0074] (1) Dipropylene glycol, 1,2-hexanediol, and deionized water were mixed uniformly, heated to 50°C, and then caffeine and nicotinamide were added and stirred at constant temperature for 50 minutes to prepare a reaction mixture;

[0075] (2) preheating the reaction mixture in step (1) to 70° C., adding glycerol polyether-26 and polyglycerol-10 diisostearate in sequence, mixing and reacting for 2 h, cooling to 48° C., adding sodium naphthalenesulfonate and stirring continuously until completely dissolved, to obtain a reaction intermediate;

[0076] (3) Add C12-14 paraffinum ether-12 and C12-13 paraffinum ether-9 to the reaction intermediate in step (2) and stir evenly, heat to 100° C., keep warm for 2 hours, and continue stirring until the material is uniform to obtain a modified caffeine mixture.

[0077] Preparation Example 1-4, a method for preparing a modified caffeine mixture, differs from Preparation Example 1-1 in that the dipropylene glycol in step (1) is replaced by an equal amount of water.

[0078] Preparation Example 1-5 is a method for preparing a modified caffeine mixture, which differs from Preparation Example 1-1 in that nicotinamide in step (1) is replaced by an equal amount of water.

[0079] Preparation Example 1-6, a method for preparing a modified caffeine mixture, differs from Preparation Example 1-1 in that an equal amount of water is added instead of C12-14 paraxene-12 and C12-13 paraxene-9 in step (3).

[0080] Preparation Example 2-1, a method for preparing a polypeptide mixture, is prepared by the following method:

[0081] 1. Preparation of phospholipid membrane: 800 g of soybean lecithin and 200 g of cholesterol were added to 220 mL of a mixture of methanol and chloroform (methanol:chloroform = 1:2) and mixed thoroughly. The mixture was maintained in a water bath at 60°C and shaken at 2000 rpm for 1.5 h to prepare a phospholipid solution.

[0082] 2. Dissolve the mixed peptides: according to the mass ratio of decapeptide-4 to oligopeptide-29 of 1:1.2, add 100g of decapeptide-4 and 120g of oligopeptide-29 into 700mL of phosphate buffer, control the water bath temperature to be maintained at 50°C and the pH value to be maintained at 7.4, stir and dissolve at a constant speed for 40 minutes to obtain a peptide mixture;

[0083] 3. Mixing and film formation: The phospholipid solution in step (1) was vacuum-extracted at a vacuum degree of 80 kPa for 100 minutes, and then the polypeptide mixture in step (2) was added and shaken and mixed at a speed of 1200 r / min for 1 hour to fully hydrate, and then emulsified and sheared at a speed of 4400 r / min for 50 minutes to obtain an emulsion;

[0084] 4. Post-treatment: The emulsion in step (3) was extruded 8 times on a liposome extruder using a 400 nm polycarbonate filter membrane. The residue was separated by centrifugation to obtain a polypeptide mixture, which was then stored at 4°C in the dark for later use.

[0085] Preparation Example 2-2, a method for preparing a polypeptide mixture, differs from Preparation Example 2-1 in that the mass ratio of decapeptide-4 and oligopeptide-29 in step (2) is 1:1.3, that is, 100g of decapeptide-4 and 130g of oligopeptide-29 are added.

[0086] Preparation Example 2-3, a method for preparing a polypeptide mixture, differs from Preparation Example 2-1 in that the mass ratio of decapeptide-4 and oligopeptide-29 in step (2) is 1:1.1, that is, 100g of decapeptide-4 and 110g of oligopeptide-29 are added.

[0087] Example

[0088] The components and weights of Examples 1-1, 1-2 and 1-3 are shown in Table 2

[0089] Table 2

[0090]

[0091]

[0092] Example 1-1, an anti-hair loss and hair growth essence, the preparation method of the anti-hair loss and hair growth essence comprises the following steps:

[0093] (1) Nicotinamide, methylpropylene glycol, PCA zinc, trehalose, betaine, citric acid, sodium citrate and 29.205 kg of deionized water were mixed uniformly, heated to 72° C., stirred and emulsified at a speed of 110 r / min, and reacted for 50 min to obtain material A;

[0094] (2) Cooling the material A in step (1) to 47° C., adding the Platycladus orientalis leaf extract, stirring at a speed of 180 r / min, and mixing thoroughly; then adding the modified caffeine mixture and mixing for 30 minutes to obtain material B;

[0095] (3) Controlling the temperature at 37° C., adding the polypeptide mixture to material B in step (2), and homogenizing for 35 minutes; then sequentially adding lemon essence pre-dissolved in PEG-40 hydrogenated castor oil, phenoxyethanol, and 35.695 kg of deionized water, and mixing and stirring for 1.5 hours. After degassing, an anti-hair loss and hair growth essence was obtained.

[0096] The modified caffeine mixture is derived from Preparation Example 1-1, and the polypeptide mixture is derived from Preparation Example 2-1.

[0097] Example 1-2, an anti-hair loss and hair growth essence, the preparation method of the anti-hair loss and hair growth essence comprises the following steps:

[0098] (1) Nicotinamide, methylpropylene glycol, PCA zinc, trehalose, betaine, citric acid, sodium citrate and 16.1 kg of deionized water were mixed uniformly, heated to 75° C., stirred and emulsified at a speed of 120 r / min, and reacted for 60 min to obtain material A;

[0099] (2) cooling the material A in step (1) to 50° C., adding the Platycladus orientalis leaf extract, stirring at a speed of 200 r / min, mixing thoroughly, and then adding the modified caffeine mixture and mixing for 40 minutes to obtain material B;

[0100] (3) Controlling the temperature at 37° C., adding the polypeptide mixture to material B in step (2), and homogenizing for 35 minutes; then sequentially adding lemon essence pre-dissolved in PEG-40 hydrogenated castor oil, phenoxyethanol, and 16.1 kg of deionized water, and mixing and stirring for 2 hours. After degassing, an anti-hair loss and hair growth essence was obtained.

[0101] The modified caffeine mixture is derived from Preparation Example 1-1, and the polypeptide mixture is derived from Preparation Example 2-1.

[0102] Example 1-3, an anti-hair loss and hair growth essence, the preparation method of the anti-hair loss and hair growth essence comprises the following steps:

[0103] (1) Nicotinamide, methylpropylene glycol, PCA zinc, trehalose, betaine, citric acid, sodium citrate and 39.34 kg of deionized water were mixed uniformly, heated to 70° C., stirred and emulsified at a speed of 100 r / min, and reacted for 50 min to obtain material A;

[0104] (2) cooling the material A in step (1) to 45° C., adding the Platycladus orientalis leaf extract, stirring at a speed of 150 r / min, mixing thoroughly, and then adding the modified caffeine mixture and mixing for 20 minutes to obtain material B;

[0105] (3) Controlling the temperature at 35° C., adding the polypeptide mixture to material B in step (2), and homogenizing for 30 minutes; then sequentially adding lemon essence pre-dissolved in PEG-40 hydrogenated castor oil, phenoxyethanol, and 59.01 kg of deionized water, and mixing and stirring for 1 hour. After degassing, an anti-hair loss and hair growth essence was obtained.

[0106] The modified caffeine mixture is derived from Preparation Example 1-1, and the polypeptide mixture is derived from Preparation Example 2-1.

[0107] Example 1-4 is an anti-hair loss and hair growth essence, which differs from Example 1-1 in that the modified caffeine mixture is derived from Preparation Example 1-2, and the polypeptide mixture is derived from 2-2.

[0108] Example 1-5 is an anti-hair loss and hair growth essence, which differs from Example 1-1 in that the modified caffeine mixture is derived from Preparation Example 1-3, and the polypeptide mixture is derived from 2-3.

[0109] Example 1-6 is an anti-hair loss and hair growth essence, which is different from Example 1-1 in that the modified caffeine mixture is derived from Preparation Example 1-4.

[0110] Example 1-7 is an anti-hair loss and hair growth essence, which is different from Example 1-1 in that the modified caffeine mixture is derived from Preparation Example 1-5.

[0111] Example 1-8 is an anti-hair loss and hair growth essence, which differs from Example 1-1 in that the modified caffeine mixture is derived from Preparation Example 1-6.

[0112] Example 1-9, an anti-hair loss and hair growth essence, differs from Example 1-1 in that the niacinamide in step (1) is replaced by an equal amount of water.

[0113] Example 1-10, an anti-hair loss and hair growth essence, is different from Example 1-1 in that the Eclipta prostrata extract in step (2) is replaced by an equal amount of water.

[0114] Example 1-11, an anti-hair loss and hair growth essence, differs from Example 1-1 in that the modified caffeine in step (2) is replaced by an equal amount of water.

[0115] Example 1-12, an anti-hair loss and hair growth essence, differs from Example 1-1 in that an equal amount of water is added instead of the polypeptide mixture in step (3).

[0116] Comparative Example

[0117] Comparative Example 1-1, an anti-hair loss and hair growth essence, differs from Example 1-1 in that, in the preparation method of the modified caffeine mixture, C12-14 pareth-12 and C12-13 pareth-9 are replaced by an equal amount of C11-12 isoparaffin.

[0118] Comparative Example 1-2 is an anti-hair loss and growth essence, which differs from Example 1-1 in that, in the preparation method of the modified caffeine mixture, dipropylene glycol is replaced by an equal amount of propylene carbonate.

[0119] Comparative Example 1-3 is an anti-hair loss and hair growth essence, which is different from Example 1-1 in that, in the preparation method of the anti-hair loss and hair growth essence, the Eclipta prostrata extract is replaced by an equal amount of Salvia miltiorrhiza root extract.

[0120] Comparative Example 1-4 is an anti-hair loss and hair growth essence, which is different from Example 1-1 in that in the preparation method of the anti-hair loss and hair growth essence, the modified caffeine mixture is replaced by an equal amount of gingerol (a hair growth hormone substance).

[0121] Performance testing

[0122] An anti-hair loss serum was prepared according to the methods described in each of the Examples and Comparative Examples. The resulting serum samples were then tested on a sample of voluntary volunteers. A total of 60 subjects were randomly selected and divided into 12 groups of 5 participants each. The subjects were male volunteers aged 40-50 years with moderate hair loss. During the test, each group member applied a sample of the anti-hair loss serum once daily, morning and evening, applying 3 mL of the sample evenly to the scalp in the area of hair loss and massaging for 5-10 minutes. No other products of the same type were used during the trial.

[0123] The test records the hair follicle density and the anti-hair loss effect. The test data are recorded in Table 3 and Table 4 respectively. The detection method is as follows:

[0124] 1. Hair follicle density: A 4cm*4cm testing area was selected at the site of hair loss on the subject's head. A hair follicle analyzer was used to capture the effective hair follicle density of the test area at 0, 2, 4, and 8 weeks.

[0125] Table 3

[0126]

[0127]

[0128] 2. Anti-hair loss effect: The number of hairs lost by the subjects when combing their hair at week 0 and week 8 was collected. Specifically, the subjects combed their hair on a 1m*1m double-layer gauze, and the number of hairs attached to the gauze was collected and counted.

[0129] Table 4

[0130]

[0131] In conjunction with Examples 1-1, 1-2, and 1-3, Comparative Example 1-1, and Tables 3 and 4, the anti-hair loss and growth essences prepared from Examples 1-1 and 1-2 increased the hair follicle density of the subjects compared to the anti-hair loss and growth essence prepared from Comparative Example 1-1. Furthermore, the anti-hair loss and growth essences prepared from Examples 1-1 and 1-2 resulted in significantly fewer hair loss strands measured by the subjects compared to the anti-hair loss and growth essence prepared from Comparative Example 1-1. A higher hair follicle density indicates a greater hair growth effect of the product essence, while a lower number of hair loss strands indicates a greater anti-hair loss effect of the product essence.

[0132] The test results show that C11-12 isoparaffins have difficulty disposing of hydrogen atoms in the mixed system and reacting with caffeine molecules. This makes it difficult to improve caffeine aggregation within the system, resulting in poor water solubility and a poor anti-hair loss and hair growth effect on the scalp. The anti-hair loss and hair growth essence prepared in Example 1-1 exhibits the best overall performance. However, the active ingredient contents in Examples 1-3 are relatively low, resulting in poor overall anti-hair loss and hair growth efficacy.

[0133] Combining Examples 1-1 and 1-2, Comparative Example 1-2, and Tables 3 and 4, the anti-hair loss and growth serums prepared from Examples 1-1 and 1-2 increased the hair follicle density in the test subjects compared to the anti-hair loss and growth serum prepared from Comparative Example 1-2. Furthermore, the anti-hair loss and growth serums prepared from Examples 1-1 and 1-2 decreased the number of hair lost in the test subjects compared to the anti-hair loss and growth serum prepared from Comparative Example 1-2. The test results indicate that propylene carbonate also struggles to contribute hydrogen atoms for effective reaction in the mixed system, resulting in poor anti-hair loss and growth efficacy of the product serums.

[0134] In combination with Examples 1-1 and 1-2, Comparative Example 1-3, and Tables 3 and 4, the anti-hair loss and hair growth essences prepared from Examples 1-1 and 1-2 increased the hair follicle density of the subjects slightly higher than that of the anti-hair loss and hair growth essences prepared from Comparative Example 1-3, and the anti-hair loss and hair growth essences prepared from Examples 1-1 and 1-2 decreased the number of hair loss measured by the subjects slightly less than that of the anti-hair loss and hair growth essences prepared from Comparative Example 1-3. The test results show that the Salvia miltiorrhiza root extract has a certain anti-hair loss effect, mainly due to its ability to promote blood circulation, dilate blood vessels, increase blood flow, and thus improve the scalp environment. However, compared to the Herba Echeveriae extract used in this application, its hair growth efficacy is slightly inferior.

[0135] In combination with Examples 1-1 and 1-2, Comparative Example 1-4, and Tables 3 and 4, the anti-hair loss and growth essences prepared from Examples 1-1 and 1-2 significantly increased the hair follicle density of the subjects compared to the anti-hair loss and growth essence prepared from Comparative Example 1-4, and the anti-hair loss and growth essences prepared from Examples 1-1 and 1-2 significantly decreased the number of hair loss measured by the subjects compared to the anti-hair loss and growth essence prepared from Comparative Example 1-3. The test results indicate that gingerol, as a hair growth hormone, has a certain hair growth effect, but its compatibility with the other components in the mixed system is poor, resulting in poor anti-hair loss and growth effects of the prepared essences.

[0136] Combining Examples 1-1, 1-4, and 1-5, as well as Tables 3 and 4, the anti-hair loss and growth serum prepared in Example 1-1 increased the hair follicle density of the subjects compared to the anti-hair loss and growth serums prepared in Examples 1-4 and 1-5, and the anti-hair loss and growth serum prepared in Example 1-1 significantly reduced the number of hair loss measured in the subjects compared to the anti-hair loss and growth serums prepared in Examples 1-4 and 1-5. The test results indicate that the amounts of each raw material added and the reaction conditions in the preparation of the modified caffeine mixture all exhibit peak values within the specified ranges. Changing the reaction conditions or approaching the end values of the added materials can affect both the yield and the efficacy of the product.

[0137] Combining Examples 1-1, 1-6, 1-7, and 1-8, as well as Tables 3 and 4, the anti-hair loss and growth serum prepared in Example 1-1 increased the hair follicle density of the subjects compared to the anti-hair loss and growth serums prepared in Examples 1-6, 1-7, and 1-8. Furthermore, the anti-hair loss and growth serum prepared in Example 1-1 significantly reduced the number of hair loss measured in the subjects compared to the anti-hair loss and growth serums prepared in Examples 1-6, 1-7, and 1-8. The test results indicate that the lack of relevant raw materials, particularly functional reagents, during the preparation of the modified caffeine mixture significantly impacts its overall anti-hair loss and growth performance, particularly the effect of the hydrogenating agent on the caffeine molecule.

[0138] Combining Examples 1-1 and 1-9, as well as Tables 3 and 4, the anti-hair loss and growth serum prepared in Example 1-1 increased the hair follicle density of the subjects compared to the anti-hair loss and growth serum prepared in Example 1-9, and the anti-hair loss and growth serum prepared in Example 1-1 significantly reduced the number of hair loss measured by the subjects compared to the anti-hair loss and growth serum prepared in Example 1-9. The test results indicate that niacinamide, whether in the modified caffeine mixture or in the main essence, can cooperate with the caffeine component to protect the scalp from UV damage, while also improving the head skin environment, thereby strengthening the barrier function and reducing scalp sensitivity.

[0139] In combination with Examples 1-1, 1-10, 1-11 and 1-12, as well as Tables 3 and 4, the anti-hair loss and hair growth essence prepared by Example 1-1 makes the hair follicle density of the subject higher than that of the anti-hair loss and hair growth essence prepared by Examples 1-10, 1-11 and 1-12, and the anti-hair loss and hair growth essence prepared by Example 1-1 makes the number of hair loss roots measured by the subject significantly less than that of the anti-hair loss and hair growth essence prepared by Examples 1-10, 1-11 and 1-12. It can be seen from the test results that the modified caffeine mixture, polypeptide mixture and plant essence in this application play a relatively important role as the three main anti-hair loss and hair growth ingredients (among which the modified caffeine mixture has the most significant effect on the effect of the product essence). The lack of any one of them will have an adverse effect on the product essence. The specific reason is that the modified caffeine mixture with a higher content of caffeine component can efficiently and stably enhance the anti-hair loss and hair growth efficacy of the product essence. At the same time, the combination of plant essence and polypeptide mixture can also nourish hair follicles, improve the scalp environment, and thus promote hair growth.

[0140] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. An anti-hair loss and hair growth essence, characterized in that: The invention is composed of the following components by weight percentage: modified caffeine mixture 0.1-5.0%; polypeptide mixture 0.1-3.0%; plant essence 0.1-20.0%; methylpropanediol 0.1-30.0%; PCA zinc 0.1-1.0%; niacinamide 0.05-2.0%; trehalose 0.5-2.0%; betaine 0.1-3.0%; daily fragrance 0.1-0.6%; PEG-40 hydrogenated castor oil 0.1-1.0%; preservative 0.2-1.0%; citric acid 0.05-0.2%; sodium citrate 0.05-0.5%; the balance is deionized water. The modified caffeine mixture comprises the following components in weight percentage: 9.2-10.0% caffeine; 9.0-10.0% nicotinamide; 4.0-5.0% dipropylene glycol; 3.8-4.0% glycerol polyether-26; 1.0-2.0% 1,2-hexanediol; 0.7-1.0% C12-14 pareth-12; 0.6-1.0% polyglycerol-10 diisostearate; 0.6-0.8% sodium naphthalenesulfonate; 0.3-0.5% C12-13 pareth-9; and the balance is deionized water. The polypeptide mixture comprises decapeptide-4 and oligopeptide-29, the mass ratio of the decapeptide-4 to the oligopeptide-29 is 1:(1.10-1.30), and the polypeptide mixture is externally coated with a phospholipid bilayer; The plant essence is one or more of Platycladus orientalis leaf extract, Polygonum multiflorum extract and Eclipta prostrata extract; The preparation method of the modified caffeine mixture comprises the following steps: S1. Evenly mix dipropylene glycol, 1,2-hexanediol, and deionized water, raise the temperature to 50-60° C., then add caffeine and nicotinamide, mix, and stir at a constant temperature for 50-90 minutes to prepare a reaction mixture; S2, preheating the reaction mixture in step S1 to 70-78 ° C, adding glycerol polyether-26 and polyglycerol-10 diisostearate in sequence, mixing and reacting for 2-3 hours, cooling to 48-54 ° C, adding sodium naphthalenesulfonate and stirring continuously until completely dissolved to obtain a reaction intermediate; S3. Add C12-14 pareth-12 and C12-13 pareth-9 to the reaction intermediate in step S2 and stir evenly. Raise the temperature to 100-110° C., keep the temperature for reaction for 2-3 hours, and continue stirring until the material is uniform to obtain a modified caffeine mixture.

2. The anti-hair loss and hair growth essence according to claim 1, characterized in that: The daily flavor is one or more of rose flavor, green tea flavor, lemon flavor and orange blossom flavor.

3. The anti-hair loss and hair growth essence according to claim 1, characterized in that: The preservative is one or more of phenoxyethanol and benzyl alcohol.

4. A method for preparing the anti-hair loss and hair growth essence according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Evenly mix niacinamide, methylpropylene glycol, PCA zinc, trehalose, betaine, citric acid, sodium citrate, and 40-50% of deionized water, heat to 70-75°C, stir and emulsify at a speed of 100-120 r / min, and react for 40-60 min to obtain material A; S2. Cooling material A in step S1 to 45-50°C, adding the plant essence, stirring at a speed of 150-200 r / min, and mixing thoroughly; then adding the modified caffeine mixture and mixing for 20-40 minutes to obtain material B; S3. Cooling material B in step S2 to 35-40° C., adding the polypeptide mixture, homogenizing and reacting for 30-40 minutes, then sequentially adding daily fragrance pre-dissolved in PEG-40 hydrogenated castor oil, preservatives, and the remaining amount of deionized water, mixing and stirring for 1-2 hours, and degassing to obtain an anti-hair loss and hair growth essence.

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