An automatic disinfection machine for plant tissue culture explants

Abstract

The present invention discloses an automatic disinfection machine for plant tissue culture explants, comprising: a water tank, a disinfection basket, solution bottles, pumps and valves; there are multiple solution bottles, and at least contain disinfectant solution and sterile water respectively; a water inlet and a water outlet are arranged on the water tank, the disinfection basket is detachably placed inside the water tank, and the disinfection basket is used for placing plant tissues; each solution bottle is connected to the water inlet through a pump, and the valve is installed at the water outlet, and the valve is used to open after a specified time after the pump is started. The pumps and valves are opened and closed by a controller to automatically pour disinfectant solution or sterile water into the inside of the water tank, and automatically discharge the disinfectant solution or sterile water inside the water tank, so as to automatically perform the disinfection and rinsing processes, without manual operation during this period, and the plant tissues are placed inside the cleaning basket and will not be washed away with the discharge of the solution.

Description

Technical Field

[0001] The present invention relates to the field of plant tissue disinfection equipment, and particularly to an automatic disinfection machine for explants in plant tissue culture. Background Art

[0002] The related technologies of the disinfection of explants in plant tissue culture disclosed by the present invention are published in:

[0003] A method for disinfecting explants in plant tissue culture disclosed in Chinese Patent Publication No. CN104106466A;

[0004] A plant tissue culture explant disinfection device disclosed in Chinese Patent Publication No. CN206658761U;

[0005] A large number of microorganisms adhere to the surface of the explants. Before inoculation and culture, strict disinfection treatment must be carried out. Generally, culture bottles or Erlenmeyer flasks are used to hold the explants. In the disinfection process, the explants are first rinsed with tap water, then quickly disinfected with 75% alcohol, rinsed with sterile water multiple times, then disinfected with 0.1% mercuric chloride or 10% sodium hypochlorite for a long time, shaken and oscillated multiple times during this period, and then rinsed with sterile water multiple times.

[0006] This operation method is basically manual and has many disadvantages. On the one hand, both the operation process and the preparation work of the preliminary supplies are relatively cumbersome; secondly, during the process of pouring the solution, the explants are easily washed away with the solution, resulting in the loss of scientific research materials; finally, due to the time required for manual operation, the explants are soaked in the sterile water that has not been fully poured for a long time before inoculation, resulting in a long water immersion time of the explants and being prone to browning in the later stage of culture. Summary of the Invention

[0007] The purpose of the present invention is to provide an automatic disinfection machine for explants in plant tissue culture to solve the problems that during the process of manually using bottles to hold, rinse, and disinfect explants, the explants are easily washed away with the solution and the explants are easily browned when soaked in the solution for a long time.

[0008] To solve the above technical problems, the present invention specifically provides the following technical solutions:

[0009] An automatic disinfection machine for plant tissue culture explants, comprising: a water tank, a disinfection basket, solution bottles, pumps and valves; there are multiple of the solution bottles, and at least contain disinfectant solution and sterile water respectively; the water tank is provided with a water inlet and a water outlet, the disinfection basket is detachably placed inside the water tank, and the disinfection basket is used for placing plant tissues; each of the solution bottles is connected to the water inlet through one of the pumps, and the pump is used to transfer the disinfectant solution or sterile water inside the solution bottle to the inside of the water tank to soak or rinse the plant tissues inside the disinfection basket; the valve is installed at the water outlet, and the valve is used to open after a specified time after the pump is started to drain the disinfectant solution or sterile water inside the water tank.

[0010] Further, the automatic disinfection machine further includes a recovery bottle, the valve is a three-way valve, the recovery bottle is connected to the water outlet through the valve, and the remaining port of the valve is connected to a drainage ditch.

[0011] Further, a first roller and a second roller are installed inside the water tank, a water tank cover is arranged on the top of the water tank, the water tank cover is detachably connected to the water tank, a third roller and a motor are installed on the water tank cover, and the motor is used to drive the third roller to rotate; the disinfection basket is in the shape of a round barrel, the disinfection basket can enclose the plant tissues inside it during rotation, when the disinfection basket is placed inside the water tank, it is supported by the first roller and the second roller, and when the water tank cover is closed, the top of the disinfection basket is pressed by the third roller; the axes of the disinfection basket, the first roller, the second roller and the third roller are parallel, and the outer circumferential surface of the disinfection basket is in frictional connection with the outer circumferential surface of the third roller, and when the motor is started, the disinfection basket rotates around its own axis in the water tank.

[0012] Further, the cross-section of the water tank is in the shape of a pentagon, the bottom wall of the water tank is horizontal and connected to the water outlet, and the side wall of the water tank includes a vertical wall and an inclined wall; wherein, the vertical wall is connected to the top of the water tank, the inclined wall is connected to the bottom surface of the water tank, the first roller and the second roller are respectively installed on two of the inclined walls, and the water inlet is connected to one of the vertical walls.

[0013] Further, both the first roller and the second roller are connected to the two inclined walls through first bearing seats, and the third roller is connected to the bottom surface of the water tank cover through a second bearing seat.

[0014] Further, the motor is fixedly connected to the water tank cover, the output shaft of the motor is connected to the input shaft of a right-angle reducer, a gear is coaxially installed on the output shaft of the right-angle reducer, the third roller includes a friction wheel part and a gear part connected coaxially, and the gear meshes with the gear part.

[0015] Furthermore, a rotating shaft seat is installed on the outer wall of the water tank. A first rotating shaft and a second rotating shaft are arranged on the rotating shaft seat. A rotating arm is installed on the water tank cover. A third rotating shaft is arranged on the rotating arm. The first rotating shaft, the second rotating shaft and the third rotating shaft are parallel. The third rotating shaft is located at a position away from the water tank cover with respect to the second rotating shaft. The first rotating shaft is higher than the second rotating shaft. The rotating arm is connected to a rotating seat through the first rotating shaft. The second rotating shaft and the third rotating shaft are connected through a telescopic rod. When the telescopic rod performs telescopic movement, the water tank cover rotates relative to the water tank with the first rotating shaft as the axis.

[0016] Furthermore, the disinfection basket includes: a top cover, an intermediate plate, a bottom plate and fasteners; the top cover is connected to the bottom plate through at least one intermediate plate. The fasteners sequentially pass through the top cover, the intermediate plate and the bottom plate and fix the three into one body. The top cover, the intermediate plate and the bottom plate are all made of mesh materials so that the disinfection basket can penetrate the solution. The top cover, the intermediate plate and the bottom plate all include a disc portion and an annular portion. The annular portion is axially extended along the edge of the disc portion. The top cover and the intermediate plate, between two adjacent intermediate plates, and the intermediate plate and the bottom plate are all connected through the annular portion, so that a flat cylindrical cleaning chamber is formed between the top cover and the intermediate plate, between two adjacent intermediate plates, and between the intermediate plate and the bottom plate. The cleaning chamber is used for placing plant tissues.

[0017] Furthermore, a first annular flange extending radially outward is formed at the edge of the top cover. A second annular flange extending radially outward is formed at the edge of the bottom plate. The axial distance between the first annular flange and the second annular flange is greater than the axial lengths of the first roller, the second roller and the third roller.

[0018] Furthermore, a plurality of axially extending sleeves are uniformly arranged in the disc portion. Two sleeves coaxial with each other in two adjacent disc portions are inserted and connected. The fasteners include bolts and nuts. The bolts pass through the plurality of sleeves and are screwed with the nuts to fix the top cover, the intermediate plate and the bottom plate into one body. The number of sleeves on the same disc portion satisfies the following condition: it is difficult for plant tissues to move radially inside the cleaning chamber.

[0019] The present application has the following beneficial effects compared with the prior art:

[0020] Provided is an automatic disinfecting machine for plant tissue culture explants. The pump and valve are opened and closed by a controller to automatically pour disinfectant or sterile water into the interior of the water tank and automatically discharge the disinfectant or sterile water inside the water tank, thereby automatically performing the disinfection and rinsing processes without manual operation during this period. Moreover, the plant tissue is placed inside the cleaning basket and will not be washed away with the discharge of the solution. Description of the Drawings

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings described below are merely exemplary. For those of ordinary skill in the art, without creative efforts, other implementation drawings can also be obtained by extending based on the provided drawings.

[0022] Figure 1 System block diagram of Embodiment 1 of the present invention;

[0023] Figure 2 Stereogram of the water tank part of Embodiment 2 of the present invention in the first working condition. In the figure, the water tank and the water tank cover are in a perspective state. The first working condition refers to the working condition where the disinfection basket is located outside the water tank and the water tank cover is opened;

[0024] Figure 3 Stereogram of the water tank part of Embodiment 2 of the present invention in the second working condition. In the figure, the water tank and the water tank cover are in a perspective state. The second working condition refers to the working condition where the disinfection basket is located inside the water tank and the water tank cover is opened;

[0025] Figure 4 Stereogram of the water tank part of Embodiment 2 of the present invention in the third working condition. The second working condition refers to the working condition where the disinfection basket is located inside the water tank and the water tank cover is closed;

[0026] Figure 5 Top view of the water tank part of Embodiment 2 of the present invention in the third working condition;

[0027] Figure 6 For Figure 5 Cross-sectional view in the A-A direction;

[0028] Figure 7 For Figure 5 Cross-sectional view in the B-B direction;

[0029] Figure 8 Stereogram of one perspective of the water tank cover of Embodiment 2 of the present invention;

[0030] Figure 9 Axial view of the cleaning basket of Embodiment 2 of the present invention, and enlarged view of its partial structure;

[0031] Figure 10 is Figure 9 a cross-sectional view in the C-C direction;

[0032] Figure 11 is the assembly drawing of the cleaning basket of Embodiment 2 of the present invention;

[0033] Figure 12 is the perspective view of the intermediate plate of Embodiment 2 of the present invention;

[0034] The reference numerals in the figure are respectively represented as follows:

[0035] 1 - water tank; 11 - water inlet; 12 - water outlet; 13 - first roller; 14 - second roller; 15 - bottom wall; 16 - side wall; 161 - vertical wall; 162 - inclined wall; 17 - rotating shaft seat; 171 - first rotating shaft; 172 - second rotating shaft; 2 - water tank cover; 21 - third roller; 211 - friction wheel part; 212 - gear part; 22 - motor; 23 - right-angle reducer; 24 - gear; 25 - rotating arm; 251 - third rotating shaft; 26 - telescopic rod; 3 - disinfection basket; 31 - top cover; 311 - first annular flange; 32 - intermediate plate; 33 - chassis; 331 - second annular flange; 34 - fastener; 341 - bolt; 342 - nut; 35 - disc part; 351 - sleeve; 36 - ring part; 37 - cleaning chamber; 38 - annular groove; 4 - solution bottle; 5 - pump; 6 - valve; 7 - recovery bottle. Embodiment

[0036] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0037] During the process of manually using a bottle to hold, rinse, and disinfect explants, the explants are likely to be washed away with the solution, and the explants are likely to turn brown when soaked in the solution for a long time.

[0038] In order to solve the above technical problems, an automatic disinfection machine for plant tissue culture explants is provided.

[0039] Embodiment 1, please refer to Figure 1 .

[0040] The automatic disinfection machine includes: a water tank 1, a disinfection basket 3, a solution bottle 4, a pump 5, and a valve 6;

[0041] There are multiple solution bottles 4, and at least they are respectively filled with disinfectant and sterile water;

[0042] The water tank 1 is provided with a water inlet 11 and a water outlet 12. The disinfection basket 3 is detachably placed inside the water tank 1, and the disinfection basket 3 is used for placing plant tissues.

[0043] Each solution bottle 4 is connected to the water inlet 11 through a pump 5. The pump 5 is used to transfer the disinfectant or sterile water inside the solution bottle 4 into the water tank 1 to soak or rinse the plant tissues inside the disinfection basket 3.

[0044] The valve 6 is installed at the water outlet 12. The valve 6 is used to open after a specified time after the pump 5 is started to drain the disinfectant or sterile water inside the water tank 1.

[0045] In Example 1, there are at least 3 solvent bottles, which are respectively filled with 75% alcohol, disinfectant (0.1% mercuric chloride or 10% sodium hypochlorite), and sterile water.

[0046] The pump 5 and the valve 6 are opened and closed by a controller. The controller is not shown in the figure. The steps of rinsing and disinfection are as follows:

[0047] Step 1: Add 75% alcohol into the water tank 1, shake the disinfection basket 3 for 30 seconds, and then empty the water tank 1.

[0048] Step 2: Add sterile water into the water tank 1, shake the disinfection basket 3 for 30 seconds, and then empty the water tank 1.

[0049] Step 3: Add disinfectant into the water tank 1, shake the disinfection basket 3 for 10 minutes, and then empty the water tank 1.

[0050] Step 4: Add sterile water into the water tank 1, shake the disinfection basket 3 for 30 seconds, and then empty the water tank 1.

[0051] In the above steps, the disinfection basket 3 can be shaken manually, or shaken by an oscillator. It is also possible not to shake the disinfection basket 3, but to use an ultrasonic cleaning device to release ultrasonic waves into the solution so that the solution and the plant tissues are in full contact.

[0052] Furthermore, since the disinfectant is likely to pollute the environment and cannot be directly discharged, to solve this problem.

[0053] The automatic disinfection machine further includes a recycling bottle 7. The valve 6 is a three-way valve. The recycling bottle 7 is connected to the water outlet 12 through the valve 6, and the remaining port of the valve 6 is connected to the drainage ditch.

[0054] In Steps 1, 2, and 4, the 75% alcohol and sterile water are directly discharged into the drainage ditch.

[0055] In Step 3, the valve 6 switches the passage, and the disinfectant is discharged into the recycling bottle 7 for recycling.

[0056] Furthermore, in Embodiment 1, the positions of the water inlet 11 and the water outlet 12 are fixed. Therefore, the water flow direction in the water tank 1 is also fixed, which causes the plant tissues inside the disinfection basket 3 to always be washed to a corner of the disinfection basket 3 by the water flow and pile up with each other. Even if the disinfection basket 3 is shaken to disperse the plant tissues inside, it is not easy to evenly distribute the plant tissues inside the solution, thus affecting the flushing and disinfection effects.

[0057] To solve this technical problem, in Embodiment 2, please refer to Figures 2 - 6 .

[0058] A first roller 13 and a second roller 14 are installed inside the water tank 1. A water tank cover 2 is provided on the top of the water tank 1. The water tank cover 2 is detachably connected to the water tank 1. A third roller 21 and a motor 22 are installed on the water tank cover 2. The motor 22 is used to drive the third roller 21 to rotate;

[0059] The disinfection basket 3 is in the shape of a round barrel. The disinfection basket 3 can enclose the plant tissues inside during rotation. When the disinfection basket 3 is placed inside the water tank 1, it is supported by the first roller 13 and the second roller 14. When the water tank cover 2 is closed, the top of the disinfection basket 3 is pressed by the third roller 21;

[0060] The axes of the disinfection basket 3, the first roller 13, the second roller 14, and the third roller 21 are parallel. The outer circumferential surface of the disinfection basket 3 is in frictional connection with the outer circumferential surface of the third roller 21. When the motor 22 is started, the disinfection basket 3 rotates around its own axis in the water tank 1.

[0061] When the plant tissues inside the disinfection basket 3 are impacted by the water flow, they always pile up below the disinfection basket 3. Along with the rotation of the disinfection basket 3, the plant tissues piled up below the disinfection basket 3 move to the top of the disinfection basket 3, then suspend in the solution and slowly fall, so that the plant tissues can be evenly distributed inside the solution.

[0062] After disinfection, just open the water tank cover 2 and take out the disinfection basket 3.

[0063] Optionally:

[0064] The cross-section of the water tank 1 is in the shape of a pentagon. The bottom wall 15 of the water tank 1 is horizontal and connected to the water outlet 12. The side wall 16 of the water tank 1 includes a vertical wall 161 and an inclined wall 162. Among them, the vertical wall 161 is connected to the top of the water tank 1, the inclined wall 162 is connected to the bottom surface of the water tank 1. The first roller 13 and the second roller 14 are respectively installed on two inclined walls 162. The water inlet 11 is connected to a vertical wall 161.

[0065] The inclined wall 162 is used to reduce the distance between the inner wall of the water tank 1 and the disinfection basket 3, thereby saving the usage amount of the disinfectant and the sterile water.

[0066] Meanwhile, the inclined wall 162 can also reduce the area of the bottom wall 15 of the water tank 1, thus facilitating the acceleration of the drainage speed.

[0067] Optional:

[0068] Both the first roller 13 and the second roller 14 are connected to the two inclined walls 162 through the first bearing seats, and the third roller 21 is connected to the bottom surface of the water tank cover 2 through the second bearing seat.

[0069] Optional:

[0070] The motor 22 is fixedly connected to the water tank cover 2. The output shaft of the motor 22 is connected to the input shaft of the right-angle reducer 23. A gear 24 is coaxially and fixedly installed on the output shaft of the right-angle reducer 23. The third roller 21 includes a friction wheel part 211 and a gear part 212 connected coaxially. The gear 24 meshes with the gear part 212.

[0071] The right-angle reducer 23 adopts worm and worm wheel transmission. Both the gear 24 and the gear part 212 adopt spur gears 24. There are two friction wheel parts 211 and they are respectively arranged at both ends of the gear part 212.

[0072] Furthermore, in Embodiment 2, the motor 22, the right-angle reducer 23, the gear 24 and the third roller 21 are all installed on the water tank cover 2, resulting in a relatively heavy weight of the water tank cover 2. Repeatedly moving the water tank cover 2 will bring a heavy physical burden to the staff.

[0073] To solve this problem, please refer to Figures 6 - 8 .

[0074] A rotating shaft seat 17 is installed on the outer wall of the water tank 1. A first rotating shaft 171 and a second rotating shaft 172 are arranged on the rotating shaft seat 17. A rotating arm 25 is installed on the water tank cover 2. A third rotating shaft 251 is arranged on the rotating arm 25. The first rotating shaft 171, the second rotating shaft 172 and the third rotating shaft 251 are parallel. The third rotating shaft 251 is located at a position away from the water tank cover 2 of the second rotating shaft 172. The first rotating shaft 171 is higher than the second rotating shaft 172;

[0075] The rotating arm 25 is connected to the rotating seat through the first rotating shaft 171. The second rotating shaft 172 and the third rotating shaft 251 are connected through a telescopic rod 26. When the telescopic rod 26 performs telescopic movement, the water tank cover 2 rotates relative to the water tank 1 with the first rotating shaft 171 as the axis.

[0076] The telescopic rod 26 adopts an electric servo push rod or a cylinder, so as to realize the automatic opening and closing of the water tank cover 2, reduce the physical consumption of the staff, and when using a cylinder to close the water tank cover 2, it can also provide an elastic force to push the third roller 21 to make it press against the outer peripheral surface of the disinfection basket 3, thereby increasing the friction between the third roller 21 and the disinfection basket 3.

[0077] Further, in Embodiment 2, a part of the plant tissue is impacted by the water flow and accumulates at the bottom of the disinfection basket 3, and is entangled or knotted with each other. At this time, even if the disinfection basket 3 is rotated, it is difficult to disperse the entangled plant tissues from each other.

[0078] To solve this problem, please refer to Figures 9 - 12 .

[0079] The disinfection basket 3 includes: a top cover 31, an intermediate disk 32, a bottom plate 33, and a fastener 34;

[0080] The top cover 31 is connected to the bottom plate 33 through at least one intermediate disk 32. The fastener 34 sequentially passes through the top cover 31, the intermediate disk 32, and the bottom plate 33 and fixes the three into one body. The top cover 31, the intermediate disk 32, and the bottom plate 33 are all made of a mesh material so that the disinfection basket 3 can pass through the solution;

[0081] The top cover 31, the intermediate disk 32, and the bottom plate 33 all include a disk portion 35 and a ring portion 36. The ring portion 36 is axially extended along the edge of the disk portion 35;

[0082] The top cover 31 and the intermediate disk 32, between two adjacent intermediate disks 32, and the intermediate disk 32 and the bottom plate 33 are all connected through the ring portion 36, so that a flat cylindrical cleaning chamber 37 is formed between the top cover 31 and the intermediate disk 32, between two adjacent intermediate disks 32, and between the intermediate disk 32 and the bottom plate 33. The cleaning chamber 37 is used to place plant tissues.

[0083] A plurality of intermediate disks 32 divide the disinfection basket 3 into a plurality of cleaning chambers 37, so that the plant tissues can be separated from each other, thereby avoiding the entanglement of the plant tissues with each other under the impact of the water flow.

[0084] Further:

[0085] A first annular flange 311 extending radially outward is formed at the edge of the top cover 31, and a second annular flange 331 extending radially outward is formed at the edge of the bottom plate 33. The outer walls of the first annular flange 311, the second annular flange 331, and the ring portion 36 constitute an annular groove 38. The axial distance between the first annular flange 311 and the second annular flange 331 is greater than the axial lengths of the first roller 13, the second roller 14, and the third roller 21.

[0086] The annular groove 38 is engaged with the first roller 13, the second roller 14, and the third roller 21, so that the first annular flange 311 and the second annular flange 331 limit the axial movement of the disinfection basket 3, so as to prevent the disinfection basket 3 from moving out of the working areas of the first roller 13, the second roller 14, and the third roller 21 during rotation.

[0087] Further:

[0088] A plurality of axially extending sleeves 351 are uniformly arranged in the disc part 35. Two sleeves 351 that are coaxial with each other in two adjacent disc parts 35 are inserted and connected. The fastener 34 includes a bolt 341 and a nut 342. The bolt 341 passes through the plurality of sleeves 351 and is screwed with the nut 342 so that the top cover 31, the middle disc 32, and the chassis 33 are fixed into one body.

[0089] The number of sleeves 351 on the same disc part 35 satisfies the following condition: it is difficult for plant tissues to move radially inside the cleaning chamber 37.

[0090] The staff can avoid the sleeves 351 and place the plant tissues on the disc part 35, and then stack the middle discs 32 layer by layer. After the disinfection basket 3 is assembled, the sleeves 351 are equivalent to partition columns inserted inside each cleaning chamber 37. During the rotation of the disinfection basket 3, it is difficult for the plant tissues inside the same cleaning chamber 37 to contact each other.

[0091] For the convenience of operation, the nut 342 is fixed to the chassis 33 with glue, and a knob is provided on the head of the bolt 341.

[0092] The above embodiments are only exemplary embodiments of the present invention and are not used to limit the present invention. The protection scope of the present invention is defined by the claims. Those skilled in the art can make various modifications or equivalent replacements within the essence and protection scope of the present invention, and such modifications or equivalent replacements should also be regarded as falling within the protection scope of the embodiments of the present invention.

Claims

1. An automatic disinfection machine for plant tissue culture explants, characterized in that it includes: a water tank, a disinfection basket, solution bottles, a pump and a valve; There are multiple of the solution bottles, and at least contain disinfectant solution and sterile water respectively; The water tank is provided with a water inlet and a water outlet. The disinfection basket is detachably placed inside the water tank, and the disinfection basket is used for placing plant tissues; Each of the solution bottles is connected to the water inlet through a pump, and the pump is used to transfer the disinfectant solution or sterile water inside the solution bottle to the inside of the water tank to soak or rinse the plant tissues inside the disinfection basket; The valve is installed at the water outlet, and the valve is used to open after a specified time after the pump is started to drain the disinfectant solution or sterile water inside the water tank; A first roller and a second roller are installed inside the water tank. A water tank cover is arranged on the top of the water tank. The water tank cover is detachably connected to the water tank. A third roller and a motor are installed on the water tank cover, and the motor is used to drive the third roller to rotate; The disinfection basket is in the shape of a round barrel. The disinfection basket can enclose the plant tissues inside it during rotation. When the disinfection basket is placed inside the water tank, it is supported by the first roller and the second roller. When the water tank cover is closed, the top of the disinfection basket is pressed by the third roller; The axes of the disinfection basket, the first roller, the second roller and the third roller are parallel. The outer circumferential surface of the disinfection basket is in frictional connection with the outer circumferential surface of the third roller. When the motor is started, the disinfection basket rotates around its own axis in the water tank; The motor is fixedly connected to the water tank cover. The output shaft of the motor is connected to the input shaft of a right-angle reducer. A gear is coaxially installed on the output shaft of the right-angle reducer. The third roller includes a friction wheel part and a gear part connected coaxially. The gear meshes with the gear part; A rotating shaft seat is installed on the outer wall of the water tank. A first rotating shaft and a second rotating shaft are arranged on the rotating shaft seat. A rotating arm is installed on the water tank cover. A third rotating shaft is arranged on the rotating arm. The first rotating shaft, the second rotating shaft and the third rotating shaft are parallel. The third rotating shaft is located at a position away from the water tank cover of the second rotating shaft, and the first rotating shaft is higher than the second rotating shaft; The rotating arm is connected to a rotating seat through the first rotating shaft. The second rotating shaft and the third rotating shaft are connected through a telescopic rod. When the telescopic rod performs a telescopic movement, the water tank cover rotates relative to the water tank with the first rotating shaft as the axis. The telescopic rod is an electric servo push rod or a cylinder.

2. The automatic disinfection machine for plant tissue culture explants according to claim 1, characterized in that the automatic disinfection machine further includes a recovery bottle. The valve is a three-way valve. The recovery bottle is connected to the water outlet through the valve, and the remaining port of the valve is connected to a drainage channel.

3. The automatic disinfection machine for plant tissue culture explants according to claim 1, characterized in that the cross-section of the water tank is in the shape of a pentagon. The bottom wall of the water tank is horizontal and connected to the water outlet. The side wall of the water tank includes a vertical wall and an inclined wall; Wherein, the vertical wall is connected to the top of the water tank, the inclined wall is connected to the bottom surface of the water tank, the first roller and the second roller are respectively installed on the two inclined walls, and the water inlet is connected to one of the vertical walls.

4. The automatic disinfection machine for plant tissue culture explants according to claim 3, wherein both the first roller and the second roller are connected to the two inclined walls through first bearing seats, and the third roller is connected to the bottom surface of the water tank cover through a second bearing seat.

5. The automatic disinfection machine for plant tissue culture explants according to claim 1, wherein the disinfection basket comprises: a top cover, an intermediate disc, a bottom plate and fasteners; the top cover is connected to the bottom plate through at least one intermediate disc, the fasteners sequentially pass through the top cover, the intermediate disc and the bottom plate and fix the three into one body, and the top cover, the intermediate disc and the bottom plate are all made of mesh materials so that the disinfection basket can penetrate the solution; the top cover, the intermediate disc and the bottom plate all comprise a disc part and an annular part, and the annular part is axially extended along the edge of the disc part; the top cover and the intermediate disc, between two adjacent intermediate discs, and the intermediate disc and the bottom plate are all connected through the annular part, so that a flat cylindrical cleaning chamber is formed between the top cover and the intermediate disc, between two adjacent intermediate discs, and between the intermediate disc and the bottom plate, and the cleaning chamber is used for placing plant tissues; a plurality of axially extended sleeves are uniformly arranged in the disc part, and two sleeves coaxial with each other in two adjacent disc parts are inserted and connected to each other. The fasteners comprise bolts and nuts, and the bolts pass through the plurality of sleeves and are spirally connected with the nuts so that the top cover, the intermediate disc and the bottom plate are fixed into one body; the number of the sleeves on the same disc part meets the following condition: it is difficult for plant tissues to radially move inside the cleaning chamber.

6. The automatic disinfection machine for plant tissue culture explants according to claim 5, wherein a first annular flange extending radially outward is formed at the edge of the top cover, a second annular flange extending radially outward is formed at the edge of the bottom plate, and the axial distance between the first annular flange and the second annular flange is greater than the axial lengths of the first roller, the second roller and the third roller.

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