Biochemistry electronic experiment device and experiment method

By designing electronic experimental devices and methods for biochemistry, the problem of insufficient staining in fluorescence microscopy was solved, enabling comprehensive observation of the structure and function of biological samples.

CN116840207BActive Publication Date: 2026-04-14NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-05
Publication Date
2026-04-14

AI Technical Summary

Technical Problem

In current biochemical experiments, fluorescence microscopy does not stain biological samples sufficiently, making it impossible to fully observe their structure and function.

Method used

An electronic experimental device for biochemistry was designed, including components such as a trough, grid column, rubber ring, and pressure strip. Through specific experimental methods, fluorescent dyes can be effectively stained to biological samples, and their fluorescence signals can be observed using a fluorescence microscope.

Benefits of technology

This method enables sufficient staining of biological samples with fluorescent dyes, ensuring comprehensive observation of the structure and function of biological samples.

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Abstract

The present application relates to the field of biochemistry electronic experiment, more particularly to a biochemistry electronic experiment device and experiment method. The electronic experiment device comprises a tank box, a plurality of grid columns are fixed on the lower side of the tank box from front to back, and a plurality of round holes are arranged on both sides of the tank box. The method comprises the following steps: S1: cutting a biological sample into multiple pieces and placing them in the tank box; S2: placing a pressing strip on the upper side of the tank box; S3: placing multiple biological samples in multiple tank boxes and arranging the multiple tank boxes between two horizontal strips; S4: driving the two horizontal strips away from each other and fixing the multiple tank boxes between the two horizontal strips; S5: placing fluorescent dye in a container with solvent, placing multiple tank boxes in the container, shaking the multiple tank boxes, and allowing the fluorescent dye to stain or label the biological sample; and S6: observing the fluorescence signal through a fluorescence microscope and observing the structure and function of the biological sample. The fluorescent dye can conveniently stain or label the biological sample.
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Description

Technical Field

[0001] This invention relates to the field of electronic experiments in biochemistry, and more specifically to an electronic experimental apparatus and method for biochemistry. Background Technology

[0002] Fluorescence microscopes are commonly used electronic experimental devices in biochemistry. They use fluorescent dyes or labels to stain or label biological samples, allowing observation of the fluorescence signal to study the structure and function of the samples. However, when observing biological samples under a fluorescence microscope, the staining may not be sufficient, making it impossible to fully observe all the structural functions of the biological sample. Summary of the Invention

[0003] To overcome the shortcomings of the prior art, the present invention provides an electronic experimental device and method for biochemistry, which has the advantage of facilitating the staining or labeling of biological samples with fluorescent dyes.

[0004] An electronic experimental device for biochemistry includes a tank, wherein multiple grid columns are fixed on the lower side of the tank from front to back, and multiple round holes are provided on both sides of the tank.

[0005] Rubber rings are glued to both the left and right sides of the slot box.

[0006] The upper cover of the slot box has a pressure strip with multiple square holes arranged from front to back.

[0007] A mounting plate is fixed to the front of the pressure strip, and the mounting plate is attached to the front side of the slot box.

[0008] An electronic experimental method for biochemistry, which uses an electronic experimental apparatus for biochemistry to conduct experiments, includes the following steps:

[0009] S1: Cut the biological sample into multiple pieces and place them in the tray;

[0010] S2: Place the pressure strip on the upper side of the slot box;

[0011] S3: Place multiple biological samples in multiple slots, and arrange the multiple slots between two horizontal bars;

[0012] S4: Drive the two crossbars away from each other, fixing multiple slot boxes between the two crossbars;

[0013] S5: Place the fluorescent dye in a container with solvent, place multiple slots in the container, and shake the multiple slots to allow the fluorescent dye to stain or label the biological sample.

[0014] S6: Observe the fluorescence signal through a fluorescence microscope to observe the structure and function of the biological sample. Attached Figure Description

[0015] The present invention will now be described in further detail with reference to the accompanying drawings and specific implementation methods.

[0016] Figure 1 A flowchart of an electronic experimental method for biochemistry;

[0017] Figure 2 A schematic diagram of the structure of an electronic experimental device for biochemistry. Figure 1 ;

[0018] Figure 3 A schematic diagram of the structure of an electronic experimental device for biochemistry. Figure 2 ;

[0019] Figure 4 A schematic diagram of the structure of an electronic experimental device for biochemistry. Figure 3 ;

[0020] Figure 5 Schematic diagram of the slot box structure Figure 1 ;

[0021] Figure 6 Schematic diagram of the slot box structure Figure 2 ;

[0022] Figure 7 Schematic diagram of the horizontal bar structure Figure 1 ;

[0023] Figure 8 Schematic diagram of the horizontal bar structure Figure 2 ;

[0024] Figure 9 Schematic diagram of the base plate and base. Figure 1 ;

[0025] Figure 10 Schematic diagram of the base plate and base. Figure 2 .

[0026] In the diagram: 101 groove box; 102 rubber ring; 103 round hole; 104 hook; 105 vertical shaft; 106 pressure strip; 107 square hole; 108 panel; 109 grid column;

[0027] 201. Horizontal bar; 202. Retaining ring; 203. Round bar; 204. L-shaped rib; 205. Rear frame; 206. Side frame; 207. Fork; 208. Protruding column; 209. T-shaped frame;

[0028] Base plate 301; sliding seat 302; tension spring 303; solid column 304; front axle 305; wave rod 306; telescopic rod 307; L-shaped column 308; sphere 309; fixed shaft 310;

[0029] Base 401; Triangular edge 402; Elastic rod 403; Stand 404. Detailed Implementation

[0030] like Figure 5-6 As shown, this example demonstrates how fluorescent dyes can be conveniently used to stain biological samples.

[0031] The electronic experimental apparatus for biochemistry includes a tray 101. Multiple grid posts 109 are welded to the lower side of the tray 101 from front to back, and multiple round holes 103 are provided on both sides of the tray 101. After a biological sample is placed inside the tray 101, the multiple grid posts 109 can block the biological sample without affecting the entry of the fluorescent dye solution into the tray 101. The fluorescent dye solution can also enter the tray 101 through the multiple round holes 103. Shaking the tray 101 allows the fluorescent dye to stain the biological sample, facilitating the staining process.

[0032] like Figure 5-6 As shown, this example can prevent collisions when multiple slot boxes 101 are placed side by side.

[0033] Since rubber rings 102 are glued to both the left and right sides of the slot box 101, when multiple slot boxes 101 are placed side by side, they can be separated by the rubber rings 102 to prevent collisions when multiple slot boxes 101 are placed side by side.

[0034] like Figure 5-6 As shown, this example can prevent biological samples from escaping from the slot 101.

[0035] The upper cover of the tray 101 has a pressure strip 106, on which multiple square holes 107 are provided from front to back. The upper cover can cover the upper side of the tray 101, thereby preventing biological samples from escaping from the tray 101. The multiple square holes 107 can also allow fluorescent dye solution to enter the tray 101 without obstructing it.

[0036] like Figure 5-6 As shown, this example can achieve the effect of correctly positioning the pressure strip 106 and the slot box 101.

[0037] Since a plate 108 is welded to the front of the pressure strip 106, and the plate 108 is attached to the front side of the slot box 101, the relative position of the pressure strip 106 and the slot box 101 is correct when the plate 108 is attached to the front side of the slot box 101.

[0038] like Figure 5-6 As shown, this example can achieve the effect of pressing the strip 106 onto the slot box 101.

[0039] Since a vertical shaft 105 is welded to one end of the slot box 101, the end of the pressure strip 106 is inserted into the vertical shaft 105. A compression spring is welded to the upper part of the vertical shaft 105, and the lower end of the compression spring presses against the upper side of the pressure strip 106. The pressure strip 106 can rotate around the vertical shaft 105, thereby controlling the opening or closing of the vertical shaft 105. The pressure strip 106 can receive the downward pressure of the compression spring, so that the pressure strip 106 presses against the slot box 101.

[0040] like Figure 5-8 As shown, this example can achieve the effect of setting multiple slot boxes 101 from left to right between two L-shaped edges 204.

[0041] Since the electronic experimental device for biochemistry also includes two horizontal bars 201, which are arranged one in front of the other, and each horizontal bar 201 has an L-shaped ridge 204 integrally formed on its upper side, and hooks 104 are welded on both the front and back sides of the slot box 101, and the two hooks 104 are hooked onto the two L-shaped ridges 204 respectively, through the cooperation of the hooks 104 and the L-shaped ridges 204, multiple slot boxes 101 can be arranged from left to right between the two L-shaped ridges 204.

[0042] like Figure 7-8 As shown, this example can achieve the effect of fixing the slot box 101 between the two horizontal bars 201.

[0043] The electronic experimental apparatus for biochemistry also includes two round rods 203, which are arranged on the left and right sides. The front ends of the two round rods 203 are slidably connected to the horizontal bar 201 located on the front side, and the rear ends of the two round rods 203 are slidably connected to the horizontal bar 201 located on the rear side. Each round rod 203 has a retaining ring 202 welded to both ends. Each round rod 203 is fitted with a compression spring 2, which is located between the two horizontal bars 201. The two compression springs 2 exert a force on the two horizontal bars 201 to move them away from each other, so that the two horizontal bars 201 always tend to move away from each other. This causes the two horizontal bars 201 to move away from each other and press the two hooks 104 on the slot box 101 respectively, thereby fixing the slot box 101 between the two horizontal bars 201.

[0044] like Figure 7-8 As shown, this example can achieve the effect of having two horizontal bars 201 tighten and fix multiple slot boxes 101.

[0045] Since the rear ends of the two round rods 203 are welded to the rear frame 205, and the left end of the rear frame 205 is welded to the side frame 206, the T-shaped frame 209 is slidably connected to the side frame 206. The T-shaped frame 209 is driven to slide by a hydraulic cylinder. Forks 207 are welded to both the front and rear ends of the T-shaped frame 209. Two protrusions 208 are welded to the left end of each crossbar 201, located in the two forks 207 respectively. The two forks 207 are arranged in a V-shape. When the T-shaped frame 209 moves to the right, it drives the two forks 207 to move to the right, so that the two forks 207 press against the two protrusions 208 respectively, thereby driving the two crossbars 201 to move closer to each other. When the T-shaped frame 209 moves to the left, it drives the two forks 207 to move to the left, so that the two forks 207 press against the two protrusions 208 respectively, thereby driving the two crossbars 201 to move away from each other. This causes the two horizontal bars 201 to tighten and fix the multiple slot boxes 101, thereby setting multiple slot boxes 101 between the two horizontal bars 201.

[0046] like Figure 5-6 As shown, this example allows for convenient control of opening or closing the pressure bar 106.

[0047] Since a handle is provided on the upper side of the pressure strip 106, the pressure strip 106 can be easily opened or closed by using the handle.

[0048] An electronic experimental method for biochemistry, which uses an electronic experimental apparatus for biochemistry to conduct experiments, includes the following steps:

[0049] S1: Cut the biological sample into multiple pieces and place them in the slot 101;

[0050] S2: Place the pressure strip 106 on the upper side of the slot box 101;

[0051] S3: Place multiple biological samples in multiple slots 101 and arrange the multiple slots 101 between two horizontal bars 201;

[0052] S4: Drive the two horizontal bars 201 away from each other, and fix the multiple slot boxes 101 between the two horizontal bars 201;

[0053] S5: Place the fluorescent dye in a container with solvent, place multiple slots 101 in the container, and shake the multiple slots 101 to stain or label the biological sample with the fluorescent dye.

[0054] S6: Observe the fluorescence signal through a fluorescence microscope to observe the structure and function of the biological sample.

[0055] An electronic experimental device for biochemistry also includes a base plate 301, a translation seat 302 slidably connected to the base plate 301 in the left-right direction, a solid column 304 slidably connected to the translation seat 302 in the vertical direction, two ends of a tension spring 303 being fixed to the solid column 304 and the translation seat 302 respectively, a front shaft 305 being welded to the front side of the solid column 304, the right end of a wave rod 306 being welded to the right end of the base plate 301, the front shaft 305 being placed on the upper side of the wave rod 306, a telescopic rod 307 being connected to the right end of the base plate 301 by screws, the movable end of the telescopic rod 307 being fixed to the translation seat 302, and the solid column 304 being connected to the side frame 206 by screws.

[0056] like Figure 9-10 As shown, this example demonstrates how to achieve thorough staining of biological samples with a convenient fluorescent dye solution.

[0057] When the telescopic rod 307 extends or retracts, it can drive the translation seat 302 to slide left and right on the base plate 301, thereby driving the solid column 304 and the front axle 305 to move left and right. The tension spring 303 always pulls the solid column 304 downward, so that the solid column 304 always has a tendency to slide downward on the translation seat 302, thereby making the front axle 305 always press on the wave rod 306. As the front axle 305 moves left and right along the wave rod 306, the front axle 305 will move up and down continuously, thereby making the solid column 304 vibrate up and down continuously, thereby driving the side frame 206, the rear frame 205 and the two crossbars 201 to vibrate up and down, thereby driving the multiple slots 101 and the biological samples inside the multiple slots 101 to vibrate up and down in the fluorescent dye solution, thereby facilitating the full staining of the biological samples by the fluorescent dye solution.

[0058] A fixed shaft 310 is welded to the lower left end of the base plate 301, and a stand 404 is welded to the left side of the base 401. The fixed shaft 310 is rotatably connected to the upper part of the stand 404 through a bearing. Multiple triangular ridges 402 are welded to the upper side of the base 401 from left to right. A ball 309 is connected to the rear side of the translation seat 302 through an L-shaped column 308. The ball 309 presses on the upper side of the multiple triangular ridges 402. The upper and lower ends of the elastic rod 403 are welded to the base plate 301 and the base 401 respectively.

[0059] like Figure 9-10 As shown, this example can further enable the fluorescent dye solution to fully stain biological samples.

[0060] The elastic rod 403 always provides an upward elastic force to the front of the base plate 301, causing the base plate 301 to always tend to rotate upward around the fixed axis 310. At this time, the ball 309 will press on the triangular edge 402. As the translation seat 302 moves left and right, the ball 309 will sweep across multiple triangular edges 402, thereby causing the base plate 301 to rotate up and down around the fixed axis 310. This drives multiple slots 101 and the biological samples inside the multiple slots 101 to shake up and down in the fluorescent dye solution, further enabling the fluorescent dye solution to fully stain the biological samples.

Claims

1. An electronic experimental apparatus for biochemistry, comprising a tank cassette (101), characterized in that: The lower side of the slot box (101) is fixed with multiple grid posts (109) from front to back, and multiple round holes (103) are provided on both sides of the slot box (101). The upper cover of the slot box (101) has a pressure strip (106), and the pressure strip (106) has multiple square holes (107) from front to back. A mounting plate (108) is fixed to the front of the pressure strip (106), and the mounting plate (108) is attached to the front side of the slot box (101); One end of the slot box (101) is fixed with a vertical shaft (105), the end of the pressure strip (106) is inserted into the vertical shaft (105), and a compression spring is fixed on the upper part of the vertical shaft (105), with the lower end of the compression spring pressing against the upper side of the pressure strip (106). It also includes two horizontal bars (201), which are arranged in front and behind. Each horizontal bar (201) has an L-shaped ridge (204) on its upper side. Hooks (104) are fixed on both the front and back sides of the slot box (101). The two hooks (104) are hooked onto the two L-shaped ridges (204) respectively. It also includes two round rods (203), which are arranged on the left and right sides. The front ends of the two round rods (203) are slidably connected to the horizontal bar (201) located on the front side, and the rear ends of the two round rods (203) are slidably connected to the horizontal bar (201) located on the rear side. Each round rod (203) has a retaining ring (202) fixed at both ends. Each round rod (203) is fitted with a compression spring II, which is located between the two horizontal bars (201). The rear ends of the two round rods (203) are fixed on the rear frame (205). The left end of the rear frame (205) is fixed with a side frame (206). The T-shaped frame (209) is slidably connected to the side frame (206). The T-shaped frame (209) is driven to slide by a hydraulic cylinder. The front and rear ends of the T-shaped frame (209) are fixed with forks (207). The left end of each crossbar (201) is fixed with a protruding post (208). The two protruding posts (208) are located in the two forks (207) respectively. It also includes a base plate (301), a sliding seat (302) that is slidably connected to the base plate (301) in the left and right directions, a solid column (304) that is slidably connected to the sliding seat (302) in the vertical direction, two ends of a tension spring (303) that are fixed to the solid column (304) and the sliding seat (302) respectively, a front axle (305) that is welded to the front side of the solid column (304), the right end of a wave rod (306) that is welded to the right end of the base plate (301), the front axle (305) that is placed on the upper side of the wave rod (306), a telescopic rod (307) that is connected to the right end of the base plate (301) by screws, the movable end of the telescopic rod (307) that is fixed to the sliding seat (302), and the solid column (304) that is connected to the side frame (206) by screws. A fixed shaft (310) is welded to the lower left end of the base plate (301), and a stand (404) is welded to the left side of the base (401). The fixed shaft (310) is rotatably connected to the upper part of the stand (404) through a bearing. Multiple triangular ridges (402) are welded to the upper side of the base (401) from left to right. A ball (309) is connected to the rear side of the translation seat (302) through an L-shaped column (308). The ball (309) presses on the upper side of the multiple triangular ridges (402). The upper and lower ends of the elastic rod (403) are welded to the base plate (301) and the base (401) respectively.

2. The electronic experimental apparatus for biochemistry according to claim 1, characterized in that: Rubber rings (102) are glued to both the left and right sides of the slot (101).

3. The electronic experimental apparatus for biochemistry according to claim 2, characterized in that: A handle is provided on the upper side of the pressure strip (106).

4. An electronic experimental method for biochemistry, characterized in that, This experimental method uses the electronic experimental apparatus for biochemistry as described in any one of claims 1-3, and includes the following steps: S1: Cut the biological sample into multiple pieces and place them in the slot (101); S2: Place the pressure strip (106) on the upper side of the slot box (101); S3: Place multiple biological samples in multiple slots (101) and set the multiple slots (101) between two horizontal bars (201); S4: Drive the two crossbars (201) away from each other, and fix the multiple slot boxes (101) between the two crossbars (201); S5: Place the fluorescent dye in a container with solvent, place multiple slots (101) in the container, and shake the multiple slots (101) to stain or label the biological sample with the fluorescent dye. S6: Observe the fluorescence signal through a fluorescence microscope to observe the structure and function of the biological sample.

Citation Information

Patent Citations

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