A microecological regulation composition, preparation method and application

Abstract

The present application relates to the field of cosmetic technology, and specifically discloses a microecological regulation composition, a preparation method and an application. A microecological regulation composition, comprising the following weight percentage components: lactobacillus fermentation lysate 0.1-5%; lactobacillus / soy milk fermentation product filtrate 0.05-2%; α-glucan oligosaccharide 0.1-10%; compound plant extract 0.2-20%; moisturizer 1-35%; solvent 0.1-10%; the remainder is deionized water; its preparation method is: lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water are stirred and mixed to obtain. The microecological regulation composition of the present application can effectively maintain the balance of skin microecology and has a prominent improvement effect on acne.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and more specifically, to a microecological regulation composition, a preparation method and an application thereof. Background Art

[0002] In recent years, affected by multiple factors such as environment and life pressure, skin problems such as acne have frequently appeared. "Acne" is the common name for acne, also known as "pimples", "acne" or "blackheads", which is a skin disease caused by the blockage and inflammation of hair follicles and sebaceous glands. Generally speaking, acne patients will have multiple types of skin lesions on their faces, some of which may be more numerous and others less numerous. Because cell renewal slows down with age, adult skin may take longer to heal than adolescent skin - this means that the marks and redness after acne may last longer, thereby increasing the phenomenon of premature skin aging.

[0003] According to the length of time that microorganisms colonize on the skin surface, they can be divided into resident flora, transient flora, and fungi, viruses, and mites. Sebaceous glands and hair follicles are the main habitats of resident flora, forming the first biological barrier of the skin, and play a role in maintaining balance and self-purification in the skin microecological environment. Propionibacterium acnes is a resident bacterium colonized in the sebaceous glands. The understanding of the role of Propionibacterium acnes in the pathophysiology of acne has changed in the past two years. It is not the over-proliferation of Propionibacterium acnes, but the loss of balance between different Propionibacterium acnes system types and the imbalance of the skin microbiome that lead to the development of acne. In fact, the skin microbial flora or skin ecological flora is a major factor in skin immune protection. Any imbalance will cause functional immune deficiency, which often leads to the occupation of skin areas by dormant pathogenic flora.

[0004] For the treatment of acne, especially moderate and severe acne, the main ingredients of medically recommended topical products are antibiotics, retinoic acid, benzoyl peroxide, acids, and traditional Chinese medicine. Currently, most cases of acne are treated with antibiotics, but the disadvantages of using antibiotics are also obvious, which may cause allergic reactions. At the same time, the flora develops resistance to drugs. For example, Propionibacterium acnes will increase its pathogenicity after becoming resistant to drugs. Long-term and excessive use will once again disrupt the balance of the normal flora system in the body and cause multiple infections.

[0005] Therefore, it is urgent to propose a solution to solve the above technical problems. Summary of the invention

[0006] In order to regulate the balance of skin microecology and effectively improve acne, the present application provides a microecology regulating composition, a preparation method and application.

[0007] In the first aspect, the present application provides a microecological regulation composition, which adopts the following technical solution:

[0008] A microecological regulation composition comprises the following components in percentage by weight:

[0009] Lactobacillus fermentation lysate 0.1-5%;

[0010] Lactobacillus / soy milk fermentation product filtrate 0.05-2%;

[0011] α-Glucan oligosaccharide 0.1-10%;

[0012] Compound plant extract 0.2-20%;

[0013] Humectant 1-35%;

[0014] Solvent 0.1-10%;

[0015] The balance was deionized water.

[0016] By adopting the above technical scheme, the lactobacillus fermentation lysate can not only inhibit the growth and reproduction of Candida albicans and Propionibacterium acnes, but also increase the relative abundance of beneficial bacteria such as Lactobacillus, Bifidobacterium, Staphylococcus, etc., and reduce the relative abundance of harmful bacteria such as Ralstonia and Pseudomonas, thereby effectively regulating the skin surface flora, inhibiting the growth of skin pathogens, maintaining the balance of skin microecology, and preventing the invasion of exogenous bacteria; the lactobacillus / soy milk fermentation product filtrate can improve the diversity of beneficial bacteria on the skin surface, maintain the healthy weak acidity of the skin, and strengthen the skin microbial barrier and chemical barrier; α-glucan oligosaccharide can competitively inhibit the proliferation of harmful bacteria through biological selectivity, regulate skin microorganisms The group maintains the diversity of beneficial bacteria and increases the relative abundance, balances the microbial flora on the skin surface, activates the expression of β-defensins, inhibits the growth of pathogens, thereby strengthening the skin's microecological barrier and making the skin healthy; and the present application uses a compound of lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide, and it is found that they can play an excellent synergistic role with each other, can inhibit the excessive proliferation of Propionibacterium acnes, participate in the information exchange between the skin and microorganisms and the skin's response reaction, improve the diversity of skin flora and the relative abundance of beneficial bacteria, promote the production of antimicrobial peptides, and then strengthen the skin's microbial barrier, which is beneficial to maintaining the balance of the skin's microecology and has a prominent improvement effect on acne.

[0017] Preferably, the compound plant extract consists of flaxseed extract and Bordeaux leaf extract, and the weight ratio of flaxseed extract to Bordeaux leaf extract is (1.3-1.9):1.

[0018] By adopting the above technical scheme, flaxseed extract has a soothing effect on inflammatory erythema caused by Propionibacterium acnes, regulates the normalization of the inherent microbial community and ecosystem of the skin, promotes the synthesis of antimicrobial peptides AMPs, optimizes the physiological function of the skin and the microbial and chemical barrier function, and better resists external invasion; Bordeaux leaf extract can specifically activate the expression of natural antimicrobial peptide HBD-3 in the skin, inhibit the excessive proliferation of Propionibacterium acnes and Staphylococcus aureus, inhibit the expression of inflammatory factors IL-8 and MIP3-α, and soothe acne redness and swelling; and the use of flaxseed extract and Bordeaux leaf extract in a specific weight range to form a composite plant extract can, on the one hand, regulate the balance of skin microecology and effectively improve acne, and on the other hand, it can also well support the compounding of lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide, and exert excellent and outstanding skin repair effects on each other.

[0019] Preferably, the weight ratio of the flaxseed extract to the Bordeaux leaf extract is 1.5:1.

[0020] By adopting the above technical scheme, the flaxseed extract and Bordeaux leaf extract in the above weight ratio can cooperate with each other to exert a relatively excellent effect when used, and can form a stable and excellent microecological regulation system with lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate, and α-glucan oligosaccharide, thereby effectively improving acne.

[0021] Preferably, the moisturizing agent is one or a combination of butylene glycol, glycerin, propylene glycol, salicylic acid and hyaluronic acid;

[0022] The solvent is one or a combination of 1,2-pentanediol, polyethylene glycol, dipropylene glycol, propylene glycol ether and dipropylene glycol oxide.

[0023] By adopting the above technical scheme, the above types of moisturizers and solvents have good dispersibility and suspension properties, and can be stably combined with other component raw materials to form an excellent mixed system, thereby being able to fully play their role to obtain a microecological regulation composition with excellent quality.

[0024] Preferably, the components of the microecological regulation composition are further added with 2-5% by weight of auxiliary additives, the auxiliary additives include silica, nano-graphene and beeswax, and the weight ratio of silica, nano-graphene and beeswax is (0.2-0.7):(0.1-0.3):1.

[0025] By adopting the above technical scheme, silica has an excellent protective effect on the skin, which is mainly reflected in physical protection, and can effectively block allergenic substances; nano-graphene has a significant antibacterial effect on harmful bacteria, and has a good preventive and improvement effect on acne skin; beeswax has a certain anti-inflammatory effect and has good product stability; and silica, nano-graphene and beeswax are composed of auxiliary additives in a specific proportion, through the micro-conduction effect of silica and nano-graphene, and using beeswax to make lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide combined with silica and nano-graphene, and then can be quickly and deeply conducted to the damaged skin, which has a significant synergistic effect on skin microecological regulation, and greatly improves the effect of the microecological regulation composition when used.

[0026] Preferably, the weight ratio of the silica, nano-graphene and beeswax is 0.4:0.2:1.

[0027] By adopting the above technical scheme, the silica, nano-graphene and beeswax in the above weight ratio have a relatively stable and excellent coordination effect with each other when used, and can form a relatively uniform coordination system with the lactobacillus fermentation lysate, the lactobacillus / soymilk fermentation product filtrate and the α-glucan oligosaccharide, thereby obtaining a microecological regulation composition with relatively excellent overall quality.

[0028] In a second aspect, the present application provides a method for preparing a microecological regulation composition, using the following technical scheme: A method for preparing a microecological regulation composition, comprising the following steps:

[0029] (1) preparing raw materials including lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water according to a ratio;

[0030] (2) The lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water in step (1) are stirred and mixed uniformly at a stirring temperature of 25-35° C., a stirring time of 5-20 min, and a stirring speed of 300-500 r / min to obtain a microecological regulation composition.

[0031] By adopting the above technical scheme, the above preparation method is simple to operate and convenient for large-scale production, and mixing the raw materials is conducive to forming a stable microecological regulation system before the composition is applied to the product, thereby being able to produce a more excellent effect.

[0032] In a third aspect, the present application provides an application of a microecological regulation composition, using the following technical solution: an application of a microecological regulation composition, including a skin care product obtained by applying the microecological regulation composition.

[0033] By adopting the above technical scheme, before existing medicine has mature microbiome modification alternative therapies for external application of acne, the ecological regulation composition of the present application can be applied to skin care products, which can serve as a supplementary means for treating acne in the medical field, and also fill this gap in the field of skin care products.

[0034] Preferably, the skin care product comprises the following components in percentage by weight:

[0035] Poloxamer 407 5-30%;

[0036] 1,3-Butanediol 1-5%;

[0037] Glycerol 0.5-10%;

[0038] Panthenol 0.1-1%;

[0039] β-Glucan 0.2-1%;

[0040] Niacinamide 1-10%;

[0041] Polyacrylate cross-linked polymer 0.2-3%;

[0042] Sodium hydroxide 0.01-0.5%;

[0043] Microecological regulation composition 0.1-10%;

[0044] p-Hydroxyacetophenone 0.5-0.8%;

[0045] 1,2-Hexanediol 0.5-0.8%;

[0046] The balance was deionized water.

[0047] By adopting the above technical scheme, the microecological regulation composition is applied to the above skin care system, which can be stably combined with other component raw materials to form a uniform mixed system, which is relatively gentle on the skin. When the above skin care products are used, they can regulate and balance the skin microbial barrier, inhibit pathogenic bacteria in the skin microecology, and effectively improve acne.

[0048] In a fourth aspect, the present application provides a method for preparing a skin care product, using the following technical solution:

[0049] A method for preparing a skin care product comprises the following steps:

[0050] (1) Prepare the raw materials of phase A, phase B, phase C and phase D according to the proportion, wherein phase A includes poloxamer 407, 1,3-butylene glycol and glycerol; phase B includes deionized water, panthenol, β-glucan, niacinamide and polyacrylate cross-linked polymer; phase C includes sodium hydroxide and microecological regulation composition; phase D includes p-hydroxyacetophenone and 1,2-hexanediol:

[0051] (2) Mix the raw materials of phase A in step (1), heat to 75-80°C, and stir evenly; mix the raw materials of phase B, heat to 75-80°C, and stir evenly; then add phase B to phase A and stir evenly;

[0052] (3) Cool the mixture of phase A and phase B in step (2) to 35-45°C, add the raw materials of phase C and phase D, stir and mix evenly, and obtain the skin care product.

[0053] By adopting the above technical scheme, the preparation process of skin care products is simple and suitable for industrial production. At the same time, in the above operation, each component raw material is added in a graded manner, which facilitates quality control during the process, thereby facilitating the acquisition of skin care products with excellent and stable quality.

[0054] In summary, this application has the following beneficial effects:

[0055] 1. The present application adopts the compound use of lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide, which can inhibit the excessive proliferation of Propionibacterium acnes, participate in the information exchange between the skin and microorganisms and the skin response reaction, improve the diversity of skin flora and the relative abundance of beneficial bacteria, promote the production of antimicrobial peptides, and then strengthen the skin microbial barrier, which is beneficial to maintain the balance of skin microecology and has a prominent improvement effect on acne;

[0056] 2. The present application preferably uses a compound plant extract composed of flaxseed extract and Bordeaux leaf extract in a specific weight range ratio, which can, on the one hand, regulate the balance of skin microecology and effectively improve acne, and on the other hand, can well support the compounding of lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide, and exert excellent and outstanding skin repair effects on each other;

[0057] 3. The present application adds auxiliary adjuvants composed of silica, nano-graphene and beeswax in a specific proportion, which can enable lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide to be quickly and deeply transmitted to damaged skin areas, which has a significant synergistic effect on skin microecological regulation and greatly improves the effect of the microecological regulation composition when applied. DETAILED DESCRIPTION

[0058] The present application is further described in detail below in conjunction with embodiments.

[0059] Unless otherwise specified, the raw materials used in the preparation examples and embodiments of the present application are commercially available:

[0060] Flaxseed extract purchased from Ashland, trade name: Lipigenine;

[0061] Product supplier of Bordeaux leaf extract: BASF, trade name: A00067 Betapur;

[0062] Product supplier of polyacrylate cross-linked polymer: Seppic, trade name: SEPIMAZEN;

[0063] The particle size of nanographene is 300nm;

[0064] Beeswax product supplier: CRODA, product name: SUPER REFINED BEESWAX-PA-(JP).

[0065] Example

[0066] Example 1

[0067] A microecological regulation composition, the raw materials of each component and their corresponding weights are shown in Table 1, and is prepared by the following steps:

[0068] (1) preparing raw materials including lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water according to a ratio;

[0069] (2) The lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water in step (1) are stirred and mixed uniformly at a stirring temperature of 25-35° C., a stirring time of 5-20 min, and a stirring speed of 300-500 r / min to obtain a microecological regulation composition.

[0070] Note: In the above steps, the stirring temperature of this embodiment is preferably 30°C, the stirring time is preferably 15 minutes, and the stirring speed is preferably 300 r / min; the compound plant extract is composed of flaxseed extract and Bordeaux leaf extract in a weight ratio of 1.5:1; the moisturizer is butanediol; and the solvent is 1,2-pentanediol.

[0071] Example 2-3

[0072] A microecological regulation composition, which is different from Example 1 in that the raw materials of its components and their corresponding weights are shown in Table 1.

[0073] Table 1 The raw materials and weight percentages of each component in Examples 1-3

[0074]

[0075] Example 4

[0076] A microecological regulation composition, which is different from Example 1 in that the compound plant extract consists of flaxseed extract and Bordeaux leaf extract in a weight ratio of 1.3:1.

[0077] Example 5

[0078] A microecological regulation composition, which is different from Example 1 in that the compound plant extract consists of flaxseed extract and Bordeaux leaf extract in a weight ratio of 1.9:1.

[0079] Example 6

[0080] A microecological regulation composition, which is different from Example 1 in that the compound plant extract consists of flaxseed extract and Bordeaux leaf extract in a weight ratio of 1.6:1.

[0081] Example 7

[0082] A microecological regulation composition, which is different from Example 1 in that the mass of flaxseed extract is replaced by deionized water.

[0083] Example 8

[0084] A microecological regulation composition, which is different from Example 1 in that the Bordeaux leaf extract is replaced by deionized water.

[0085] Example 9

[0086] A microecological regulation composition, which is different from Example 1 in that the compound plant extract consists of flaxseed extract and Bordeaux leaf extract in a weight ratio of 1.2:1.

[0087] Example 10

[0088] A microecological regulation composition, which is different from Example 1 in that the compound plant extract consists of flaxseed extract and Bordeaux leaf extract in a weight ratio of 2.0:1.

[0089] Embodiment 11

[0090] A microecological regulation composition, which is different from Example 1 in that the moisturizer is a composition of glycerol and hyaluronic acid in a weight ratio of 1:1.

[0091] Example 12

[0092] A microecological regulation composition, which is different from Example 1 in that the solvent is a composition of dipropylene glycol and dipropylene glycol oxide in a weight ratio of 1:1.

[0093] Example 13

[0094] A microecological regulation composition, which is different from Example 1 in that the raw materials in step (1) also contain 2-5% by weight of an auxiliary agent, the auxiliary agent is composed of silica, nanographene and beeswax in a weight ratio of 0.4:0.2:1, and is mixed with other component raw materials in step (1) when used. In this embodiment, 3% of the auxiliary agent is preferred.

[0095] Embodiment 14

[0096] A microecological regulation composition, which is different from Example 13 in that the auxiliary agent is composed of silica, nano-graphene and beeswax in a weight ratio of 0.2:0.1:1.

[0097] Embodiment 15

[0098] A microecological regulation composition, which is different from Example 13 in that the auxiliary agent is composed of silica, nano-graphene and beeswax in a weight ratio of 0.7:0.3:1.

[0099] Example 16

[0100] A microecological regulation composition, which is different from Example 13 in that the auxiliary agent is composed of silica, nano-graphene and beeswax in a weight ratio of 0.45:0.2:1.

[0101] Embodiment 17

[0102] A microecological regulating composition, which differs from Example 13 in that the mass of silica is replaced by deionized water.

[0103] Embodiment 18

[0104] A microecological regulating composition, which is different from Example 13 in that the mass of nanographene is replaced by deionized water.

[0105] Embodiment 19

[0106] A microecological regulation composition, which is different from Example 13 in that the mass of beeswax is replaced by deionized water.

[0107] Embodiment 20

[0108] A microecological regulation composition, which is different from Example 13 in that the silica, nanographene and other materials are replaced by deionized water.

[0109] Embodiment 21

[0110] A microecological regulation composition, which is different from Example 13 in that silica, beeswax and other materials are replaced by deionized water.

[0111] Embodiment 22

[0112] A microecological regulation composition, which is different from Example 13 in that nanographene, beeswax and other materials are replaced by deionized water.

[0113] Embodiment 23

[0114] A skin care product, the raw materials of each component and their corresponding weights are shown in Table 1, and is prepared by the following steps:

[0115] (1) Prepare the raw materials of phase A, phase B, phase C and phase D according to the proportion, wherein phase A includes poloxamer 407, 1,3-butylene glycol and glycerol; phase B includes deionized water, panthenol, β-glucan, niacinamide and polyacrylate cross-linked polymer; phase C includes sodium hydroxide and microecological regulation composition; phase D includes p-hydroxyacetophenone and 1,2-hexanediol:

[0116] (2) Mix the raw materials of phase A in step (1), heat to 75-80°C, stir evenly, and stir at 300 r / min for 10 min; mix the raw materials of phase B, heat to 75-80°C, stir evenly, and stir at 300 r / min for 10 min; then add phase B to phase A, stir evenly, and stir at 300 r / min for 5 min;

[0117] (3) Cooling the mixture of phase A and phase B in step (2) to 35-45° C., adding the raw materials of phase C and phase D, stirring and mixing evenly, stirring at 300 r / min for 15 min, and obtaining a skin care product.

[0118] Note: In the above operation, both phase A and phase B are heated to 80°C; the mixture of phase A and phase B is preferably cooled to 40°C; the microecological regulation composition is obtained in Preparation Example 1.

[0119] Examples 24-25

[0120] A skin care product, which is different from Example 23 in that its component raw materials and their corresponding weights are as shown in Table 2.

[0121] Table 2 Raw materials and weight percentages of components in Examples 23-25

[0122]

[0123] Embodiment 26

[0124] A skin care product, which is different from Example 23 in that the microecological regulation composition is obtained according to Example 11, and part of the deionized water is removed to ensure that the percentage content of lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate and α-glucan oligosaccharide is the same as that of Example 21.

[0125] Comparative Example

[0126] Comparative Example 1

[0127] A microecological regulation composition, which is different from Example 1 in that an equal mass of lactobacillus fermentation lysate is replaced by deionized water.

[0128] Comparative Example 2

[0129] A microecological regulation composition, which is different from Example 1 in that an equal mass of the lactobacillus / soymilk fermentation product filtrate is replaced with deionized water.

[0130] Comparative Example 3

[0131] A microecological regulation composition, which is different from Example 1 in that the mass of α-glucan oligosaccharide is replaced by deionized water.

[0132] Comparative Example 4

[0133] A microecological regulation composition, which is different from Example 1 in that the lactobacillus fermentation lysate, the lactobacillus / soymilk fermentation product filtrate and α-glucan oligosaccharide are replaced by deionized water in equal amounts.

[0134] Comparative Example 5

[0135] A microecological regulation composition, which is different from Example 1 in that the mass of the compound plant extract is replaced by deionized water.

[0136] Performance detection test samples: the microecological regulation composition obtained in Examples 1-22 was used as test samples 1-22, the skin care products obtained in Examples 22-26 were used as test samples 22-26, and the microecological regulation composition obtained in Comparative Examples 1-5 was used as control samples 1-5.

[0137] Test method:

[0138] Selection of testers: ① Inclusion criteria: 62 acne patients aged 18 to 50 years old, the test site is within 1 cm extending outward from the skin where the acne is located, and those who can strictly abide by the experimental requirements and sign the informed consent form of volunteers. ② Exclusion criteria: pregnant or breastfeeding women; those who are allergic or highly sensitive to toiletries; those with immune deficiencies or other skin diseases in the test site. ③ Exclusion criteria: those who actively apply to withdraw during the experiment, poor compliance, pregnancy, adverse reactions, loss of follow-up, or other special circumstances require withdrawal from the experiment.

[0139] (1) Inhibitory test of Propionibacterium acnes, 62 people were divided into two groups, with one male and one female in each group; 31 groups of people, each group of people took 0.1mL of tissue fluid from the test site, added 1mL of normal saline to dilute 10 times, mixed and inoculated into 5 agar culture media. After 7 days of culture, the number of colonies was counted and the average value was recorded as the initial value of each person. The initial values ​​of the two people in each group were added and averaged, and the corresponding samples were recorded as A1; then the 31 groups of people smeared the test samples 1-26 and the control samples 1-5 once at 8 am and 8 pm respectively, with the application amount of 2 mg / cm 2 After continuous application for 15 days, the initial value of the colony count for each person was determined by the same method. The initial values ​​of the two people in each group were added together and averaged, and the corresponding samples were recorded as A2; and the Propionibacterium acnes inhibition rate (%) = (A1-A2) / A1, recorded in Table 3.

[0140] (2) hBD-2 test: hBD-2 is mainly expressed in epithelial tissues and is synthesized and secreted by keratinocytes. It is a cationic small molecule antimicrobial peptide that protects the skin from invasion by foreign pathogens. The excellence of the skin microecology is closely related to the expression of hBD-2. 31 groups of people, each group of people took 0.1mL of tissue fluid from the test site, added 1mL of normal saline to dilute 10 times, and the hBD-2 expression level was detected by Elisa method and fluorescent quantitative PCR detection method. The hBD-2 expression levels of two people in each group were added and averaged, and the corresponding samples were recorded as B1. Then, the 31 groups of people applied test samples 1-26 and control samples 1-5 once at 8 am and 8 pm respectively, with an application amount of 2 mg / cm 2 After 17 days of continuous application, the hBD-2 expression level of each person was determined by the same method, and the average value of each group was taken as B2 for each sample; the hBD-2 expression level change rate (%) = (B2-B1) / B1, recorded in Table 3.

[0141] Table 3 Test results of test samples 1-26 and control samples 1-5

[0142]

[0143]

[0144] It can be seen from Example 1 and Comparative Examples 1-4 and Table 3 that the lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate and α-glucan oligosaccharide can play an excellent composite synergistic effect, which can not only inhibit and reduce Propionibacterium acnes, effectively improve acne, but also regulate the skin microecology, making the skin microecology more excellent, so that the hBD-2 expression amount is more prominent. While using any one of the lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate and α-glucan oligosaccharide alone, compared with the case where none of the three raw materials are used, although the inhibition rate of Propionibacterium acnes and the change rate of hBD-2 expression can be improved, the improvement effect is limited, far less than the excellent effect brought by the composite use of the three raw materials.

[0145] In combination with Example 1, Example 4-6 and Table 3, it can be seen that the use of flaxseed extract and Bordeaux leaf extract in a specific weight range ratio to form a composite plant extract can make the obtained microecological regulation composition maintain an excellent and stable application effect, wherein, when the weight ratio of flaxseed extract and Bordeaux leaf extract is 1.5: 1, the effect is the most excellent. In combination with Example 7-8, Comparative Example 5 and Table 3, it can be seen that the combination of flaxseed extract and Bordeaux leaf extract can play a role in compounding and enhancing. In combination with Example 9-10 and Table 3, it can be seen that when the ratio between flaxseed extract and Bordeaux leaf extract is lower than or higher than (1.3-1.9): 1, the application effect of the microecological regulation composition is significantly lost, indicating that the ratio between the two is of high importance. Combined with Comparative Example 4, Comparative Example 5 and Table 3, it can be seen that when the lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate and α-glucan oligosaccharide are not used, the effect of using the composite plant extract alone is far less than the effect brought about by the application of the composite plant extract in Example 1, indicating that the use of the composite plant extract and the cooperation between the lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate and α-glucan oligosaccharide have excellent and outstanding effects.

[0146] It can be seen from Example 1, Example 13-22 and Table 3 that the addition of an auxiliary agent composed of silica, nanographene and beeswax in a specific ratio can further improve the application effect of the microecological regulation composition, and can significantly improve the inhibition rate of Propionibacterium acnes and the hBD-2 expression change rate obtained by the test, wherein when the weight ratio of silica, nanographene and beeswax is 0.4:0.2:1, the improvement effect is better. However, adding any one or any two of silica, nanographene and beeswax for use, first, the improvement effect is limited, and second, it can only play a superposition of the improvement effect. Only when the three raw materials are compounded can the technical effect of 1+1>2 be brought.

[0147] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A microecological regulation composition, characterized in that: The microecological regulation composition comprises the following components in percentage by weight: Lactobacillus fermentation lysate 0.1-5%; Lactobacillus / soy milk fermentation product filtrate 0.05-2%; α-Glucan oligosaccharide 0.1-10%; Compound plant extract 0.2-20%; Moisturizer 1-35%; Solvent 0.1-10%; The balance is deionized water; The compound plant extract consists of flax seed extract and Bordeaux leaf extract, and the weight ratio of the flax seed extract to the Bordeaux leaf extract is (1.3-1.9):

1.

2. The microecological regulation composition according to claim 1, characterized in that: The weight ratio of the flaxseed extract to the Bordeaux leaf extract is 1.5:

1.

3. The microecological regulation composition according to claim 1, characterized in that: The moisturizing agent is one or a combination of butylene glycol, glycerin, propylene glycol, salicylic acid and hyaluronic acid; The solvent is one of 1,2-pentanediol and dipropylene glycol or a combination of the two.

4. The microecological regulation composition according to claim 1, characterized in that: The components of the microecological regulation composition are also added with 2-5% by weight of auxiliary additives, wherein the auxiliary additives include silica, nano-graphene and beeswax, and the weight ratio of silica, nano-graphene and beeswax is (0.2-0.7): (0.1-0.3):

1.

5. The microecological regulation composition according to claim 4, characterized in that: The weight ratio of the silica, nano-graphene and beeswax is 0.4:0.2:

1.

6. The method for preparing the microecological regulation composition according to claim 1, characterized in that: The following steps are involved: (1) preparing raw materials including lactobacillus fermentation lysate, lactobacillus / soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water according to the ratio; (2) The lactobacillus fermentation lysate, lactobacillus / soy milk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water in step (1) are stirred and mixed uniformly at a stirring temperature of 25-35° C., a stirring time of 5-20 min, and a stirring speed of 300-500 r / min to obtain a microecological regulation composition.

7. The use of the microecological regulation composition according to any one of claims 1 to 5, characterized in that: The invention comprises skin care products obtained by applying the microecological regulating composition.

8. The use of the microecological regulation composition according to claim 7, characterized in that: The skin care product comprises the following components in weight percentage: Poloxamer 407 5-30%; 1,3-Butanediol 1-5%; Glycerol 0.5-10%; Panthenol 0.1-1%; β-Glucan 0.2-1%; Niacinamide 1-10%; Polyacrylate cross-linked polymer 0.2-3%; Sodium hydroxide 0.01-0.5%; Microecological regulation composition 0.1-10%; p-Hydroxyacetophenone 0.5-0.8%; 1,2-Hexanediol 0.5-0.8%; The balance was deionized water.

9. The method for preparing the skin care product according to claim 8, characterized in that: The following steps are involved: (1) Prepare the raw materials of phase A, phase B, phase C and phase D according to the proportion, wherein phase A includes poloxamer 407, 1,3-butylene glycol and glycerol; phase B includes deionized water, panthenol, β-glucan, niacinamide and polyacrylate cross-linked polymer; phase C includes sodium hydroxide and microecological regulation composition; phase D includes p-hydroxyacetophenone and 1,2-hexanediol: (2) Mix the raw materials of phase A in step (1), heat to 75-80°C, and stir evenly; mix the raw materials of phase B, heat to 75-80°C, and stir evenly; then add phase B to phase A and stir evenly; (3) Cooling the mixture of phase A and phase B in step (2) to 35-45° C., adding the raw materials of phase C and phase D, stirring and mixing evenly, and obtaining a skin care product.

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