A fungicide composition for preventing and controlling avocado anthracnose

Abstract

The present invention belongs to the technical field of fungicide research and development, and particularly relates to a fungicide composition for controlling avocado anthracnose. A fungicide composition for controlling avocado anthracnose, wherein the active ingredient of the fungicide composition is composed of allicin compounded with fluxapyroxad, dimethomorph or bromothalonil. After the active ingredient allicin of the fungicide composition of the present invention is compounded with fluxapyroxad, dimethomorph or bromothalonil, it shows excellent antibacterial activity against Colletotrichum gloeosporioides causing avocado anthracnose. When compounded in a certain mass ratio, the co-toxicity coefficient against Colletotrichum gloeosporioides is greater than 120, showing a synergistic effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of fungicide research and development, and particularly relates to a fungicide composition for controlling avocado anthracnose. Background Art

[0002] Allicin is a plant-derived antibiotic extracted from garlic. Its biological activity mainly comes from the interaction with sulfhydryl compounds and antioxidant capacity. The mechanism of its antibacterial action may be the inhibitory effect on acetyl coenzyme A. Research shows that allicin can inhibit the mycelial growth, spore production and spore germination of pathogenic fungi of pesticide fungal diseases.

[0003] fluxapyroxad is a succinate dehydrogenase inhibitor (SDHI) fungicide with a pyrazole amide structure discovered, developed and produced by Syngenta. As an SDHI fungicide, fluxapyroxad is also an inhibitor of the respiratory action of pathogenic bacteria. It inhibits the function of mitochondria by interfering with the tricarboxylic acid cycle on electron transport chain complex II, preventing it from generating energy, thereby inhibiting the growth of pathogenic bacteria and ultimately causing its death. Fluxapyroxad is highly efficient and broad-spectrum, with protective and therapeutic effects, and can be used to control various fungal diseases on various plants.

[0004] Dimethomorph is a broad-spectrum morpholine fungicide with a unique mode of action against fungi of the Peronosporaceae family of oomycetes and Phytophthora cinnamomi. It mainly causes the decomposition of the sporangial wall, thereby killing the mycelium. Except for the formation and swimming period of zoospores, it acts on all stages of the oomycete life cycle, especially more sensitive to the formation stage of sporangiophores and oospores. It is particularly effective against downy mildew, Phytophthora and Pythium fungi, and can be used to control fungal diseases on various plants.

[0005] Bromothalonil is a broad-spectrum, low-toxic, mildew-proof and algaecidal fungicide. It can inhibit and eradicate the growth of bacteria, fungi and algae, and is suitable for the anti-corrosion and mildew-proof of textiles and leather, etc., and the algaecide of industrial water. And it has a good control effect on crop diseases, especially has a special effect on anthracnose.

[0006] During the process of fruit tree planting, fungicides are often sprayed to control fungal and bacterial diseases on fruit trees. However, long-term single use of a certain fungicide will lead to the generation of drug resistance of pathogenic bacteria, and then cause a series of problems such as environmental pollution, pesticide residues and the aggravation of drug resistance. Scientific and reasonable compounding of bactericidal active ingredients can expand the bactericidal spectrum and improve the control effect, and can also delay the occurrence and development of drug resistance of pathogenic bacteria. At present, there is no relevant report on the compounding of allicin with fluxapyroxad, dimethomorph or bromothalonil.

[0007] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention

[0008] The object of the present invention is to provide a fungicide composition for preventing and controlling avocado anthracnose, so as to solve the problems caused by the long-term single use of a certain fungicide.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A fungicide composition, wherein the active ingredients of the fungicide composition are compounded by allicin and fluxapyroxad, dimethomorph or bromothalonil.

[0011] Preferably, the mass ratio of allicin to fluxapyroxad is 1-7:7-1.

[0012] Preferably, the mass ratio of allicin to dimethomorph is 1-15:20-1.

[0013] Preferably, the mass ratio of allicin to bromothalonil is 1:25-1.

[0014] The present invention also provides the use of the described fungicide composition in preventing and controlling avocado anthracnose.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) After the compounding of the active ingredient allicin of the fungicide composition of the present invention with fluxapyroxad, dimethomorph or bromothalonil, it shows excellent antibacterial activity against Colletotrichum gloeosporioides of avocado anthracnose. The co-toxicity coefficient of the compounding with a certain mass ratio against Colletotrichum gloeosporioides is greater than 120, showing a synergistic effect. Compared with a single active ingredient, the control effect can be improved, and the dosage and pesticide residue can be reduced.

[0017] (2) The fungicide composition of the present invention can delay the occurrence and development of pathogen resistance, extend the service life of the medicament, and provide support for preventing and controlling drug-resistant diseases. Detailed Embodiments

[0018] The technical solutions of the present invention will be clearly and completely described below. Obviously, the described embodiments are some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work belong to the scope of protection of the present invention.

[0019] Example : Screening of Control Agents

[0020] 1. Test Strains

[0021] Collect fruit disease samples of avocado anthracnose, isolate Colletotrichum gloeosporioides penz. in the laboratory, and propagate and culture it on PDA medium for 3 days for standby.

[0022] 2. Test agents

[0023] 98% allicin technical (CAS: 539-86-6), 95% fluxapyroxad technical, 98% dimethomorph technical, 95% bromothalonil technical. All of the above agents are commercially available.

[0024] Dissolve the test agents and dilute them with 0.1% Tween-80 aqueous solution into single-agent stock solutions, and then set multiple groups of ratios. Five mass concentration gradients are set for each single-agent stock solution and the mixed agents of the ratios according to the equal ratio method.

[0025] 3. Test methods

[0026] The mycelial growth rate method is adopted. Pour 90 mL of melted PDA medium cooled to about 55 °C into a sterile conical flask, then pipette 10 mL of the liquid medicine into the above conical flask, shake well and pour it equally into 10 petri dishes with a diameter of 9 cm to make drug-containing plates with corresponding concentrations; and set a treatment without the agent as a blank control, and 10 plates are set for each mass concentration of the liquid medicine. Use a puncher to cut a mycelial disc with a diameter of 5 mm at the edge of the colony of the tested strain after propagation, and inoculate the mycelial disc in the center of the drug-containing plate and the blank control plate. Cover the petri dish lid and place it in an incubator at 25 °C for cultivation. After 3 days, measure the colony diameter by the cross method and calculate the inhibition rate of mycelial growth for different treatments.

[0027]

[0028] 4. Data analysis

[0029] Use DPS software for data statistical analysis to obtain the virulence regression equation and the virulence EC 50 value of the agent against the target pathogen, and calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method to evaluate the synergistic effect of the agent. The results are shown in Tables 1-3.

[0030] Table 1 Toxicity of the combination of allicin and fluxapyroxad against Colletotrichum gloeosporioides causing avocado anthracnose

[0031] Name and ratio of medicaments EC50 (mg / L) ATI TTI CTC Allitridum 126.04 100.00 -- -- Fluxapyroxad 1.17 10772.65 -- -- Allitridum 1: Fluxapyroxad 7 0.78 16158.97 9438.57 171.20 Allitridum 1: Fluxapyroxad 3 1.05 12003.81 8104.49 148.11 Allitridum 1: Fluxapyroxad 1 1.87 6740.11 5436.32 123.98 Allitridum 3: Fluxapyroxad 1 3.63 3472.18 2768.16 125.43 Allitridum 7: Fluxapyroxad 1 6.59 1912.59 1434.08 133.37

[0032] As can be seen from Table 1, the combination of allicin and fluxapyroxad showed excellent antibacterial activity against Colletotrichum gloeosporioides causing avocado anthracnose. The co-toxicity coefficients of all test combinations against Colletotrichum gloeosporioides were greater than 120, showing a synergistic effect.

[0033] Table 2 Toxicity of the combination of allicin and dimethomorph against Colletotrichum gloeosporioides causing avocado anthracnose

[0034]

[0035]

[0036] As can be seen from Table 2, the combination of allicin and dimethomorph showed excellent antibacterial activity against Colletotrichum gloeosporioides causing avocado anthracnose. The co-toxicity coefficients of all test combinations against Colletotrichum gloeosporioides were greater than 120, showing a synergistic effect.

[0037] Table 3 Toxicity of the combination of allicin and bromothalonil against Colletotrichum gloeosporioides causing avocado anthracnose

[0038] Name and ratio of medicaments EC50 (mg / L) ATI TTI CTC Allitridum 126.04 100.00 -- -- Bromothalonil 3.25 3878.15 -- -- Allitridum 1: Bromothalonil 25 2.55 4942.75 3732.84 132.41 Allitridum 1: Bromothalonil 15 0.74 17032.43 3642.02 467.66 Allitridum 1: Bromothalonil 7 1.26 10003.17 3405.88 293.70 Allitridum 1: Bromothalonil 3 3.15 4001.27 2933.62 136.39 Allitridum 1: Bromothalonil 1 5.21 2419.19 1989.08 121.62

[0039] As can be seen from Table 3, the combination of allicin and bromothalonil showed excellent antibacterial activity against Colletotrichum gloeosporioides causing avocado anthracnose. The co-toxicity coefficients of all test combinations against Colletotrichum gloeosporioides were greater than 120, showing a synergistic effect.

[0040] In summary, the combination of allicin and fluxapyroxad, dimethomorph or bromothalonil showed excellent antibacterial activity against Colletotrichum gloeosporioides causing avocado anthracnose. The co-toxicity coefficients of all combinations at a certain mass ratio against Colletotrichum gloeosporioides were greater than 120, showing a synergistic effect. Compared with a single active ingredient, it can improve the control effect, reduce the dosage and pesticide residues.

[0041] The foregoing description of specific exemplary embodiments of the invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and the purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical applications, so that those skilled in the art can implement and utilize various different exemplary embodiments of the invention as well as various different selections and changes. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A fungicide composition, characterized in that, The active ingredients of the fungicide composition are compounded by allicin and fluxapyroxad, and the mass ratio of allicin to fluxapyroxad is 1-7:7-1.

2. Use of the fungicide composition according to claim 1 in controlling avocado anthracnose.

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