An oral liquid for treating purpura

Abstract

The application discloses an oral liquid for treating purpura, which is prepared by high-temperature extraction of red tea with yellow rice wine and clarification of the extracted liquid. The oral liquid is prepared by taking red tea and yellow rice wine as raw materials, can effectively treat thrombocytopenic purpura, allergic purpura and capillary bleeding caused by thrombocytopenia due to primary diseases, has small toxic and side effects and high efficiency, and is in the form of stable oral liquid, so that the oral liquid is convenient to take, carry and store.

Description

Technical Field

[0001] This invention relates to the field of oral liquid preparation technology, and in particular to an oral liquid for treating purpura. Background Technology

[0002] Purpura, also known as petechiae, is characterized by petechiae and ecchymoses caused by bleeding under the skin and mucous membranes. These petechiae do not blanch upon pressure and are a common hemorrhagic disease in children. Purpura is generally divided into allergic purpura and thrombocytopenic purpura. Allergic purpura is usually treated with steroid immunosuppressants and anti-allergy medications; thrombocytopenic purpura is generally treated with immunosuppressants, such as corticosteroids. However, steroid treatment has significant side effects. Long-term use can cause immunosuppression, leading to bone marrow suppression, liver and kidney damage, femoral head necrosis, and immune system disorders, all of which are detrimental to the patient's health.

[0003] Currently, there are some traditional Chinese medicine formulas for treating purpura. For example, the "Traditional Chinese Medicine Preparation for Treating Thrombocytopenic Purpura" disclosed in Chinese patent literature, publication number CN104547554B, includes ingredients such as Phytolacca acinosa, Agrimonia pilosa, Indigo naturalis, Lithospermum erythrorhizon, Sanguisorba officinalis, Rheum palmatum, Codonopsis pilosula, Colla corii asini, Carthamus tinctorius, Ilex chinensis, Eclipta prostrata, Polygonatum sibiricum, Rubia cordifolia, Lycium chinense root bark, Cimicifuga foetida, Cicadae periostracum, Cnidium monnieri, and Bombyx mori. However, these traditional Chinese medicine preparations contain a large number of drug components, making it difficult to avoid toxic side effects on the human body. Summary of the Invention

[0004] This invention aims to overcome the aforementioned problems of existing drugs for treating purpura and provides an oral liquid for treating purpura. The oral liquid is made from black tea and rice wine as raw materials and can effectively treat thrombocytopenic purpura, allergic purpura, and capillary bleeding caused by thrombocytopenia due to primary diseases. Using food as a substitute for medicine as raw materials results in fewer toxic side effects and a higher efficacy rate. Furthermore, the stable oral liquid form makes it convenient to take, carry, and store.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An oral liquid for treating purpura is made by extracting black tea at high temperature with rice wine and then clarifying the extract.

[0007] Black tea is warm in nature and has the effect of promoting blood circulation and removing blood stasis. It is also rich in tannins and other components with astringent and hemostatic effects, which can prevent capillary bleeding. Extracting black tea with rice wine helps promote blood circulation, allowing the components to enter the bloodstream and facilitating the distribution of the medicine. Therefore, this invention uses rice wine to extract black tea at high temperatures to produce an oral liquid, which can effectively treat thrombocytopenic purpura, allergic purpura, and capillary bleeding caused by thrombocytopenia due to primary diseases. It shows effects in three days and can cure the condition in two weeks, with a high efficacy rate. Furthermore, both the black tea and rice wine used as raw materials are food products, and using food as a substitute for medicine reduces toxicity and side effects.

[0008] Furthermore, this invention uses high-temperature extraction to produce an oral liquid, which is absorbed quickly, convenient to carry and take, and easy to store. However, when extracting black tea with rice wine, the rice wine contains various unstable substances such as proteins, polysaccharides, tannins, and iron ions. The black tea extract is also rich in polyphenols, caffeine, proteins, and metal ions. Polyphenols easily react with proteins, polysaccharides, and caffeine to produce precipitation. Therefore, the extract obtained by this invention is prone to precipitation after cooling over time, making the oral liquid unstable, easily becoming cloudy, and affecting its efficacy and shelf life. Therefore, this invention clarifies the extract after high-temperature extraction to prevent precipitation during storage after cooling, thus improving the quality stability of the oral liquid and extending its shelf life.

[0009] As a preferred method, the high-temperature extraction method is as follows: add black tea to rice wine, boil and extract for 1 to 3 hours, and then filter to obtain black tea residue and extract.

[0010] As a preferred option, the ratio of black tea to rice wine is 1g:20-30mL.

[0011] As a preferred method, the clarification of the extract is as follows: a clarifying agent is added to the extract, and the mixture is stirred and reacted at 40-50°C for 2-4 hours. After filtration, the oral liquid for treating purpura is obtained.

[0012] Preferably, the method for preparing the clarifying agent includes the following steps:

[0013] (1) The extracted black tea residue is dried and pulverized to obtain black tea residue powder;

[0014] (2) Add black tea residue powder and furfural to ethanol, stir and disperse evenly, then add hydrochloric acid solution, heat and stir to react, filter, wash and dry the product to obtain furfural modified black tea residue.

[0015] (3) Disperse furfural-modified black tea residue in deionized water to obtain an aqueous phase;

[0016] (4) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain the oil phase.

[0017] (5) The oil phase is added dropwise to the aqueous phase under stirring. After stirring and homogenizing, an emulsion is obtained. The emulsion is heated at 60-80℃ for 18-24 hours. The product is dried to obtain the carrier.

[0018] (6) Add tanninase to phosphate buffer solution with pH 4-6 to obtain tanninase solution; add carrier to tanninase solution, shake reaction, filter, wash and dry the product to obtain the clarifying agent.

[0019] This invention modifies the remaining black tea residue after extraction to create a carrier, which is then used to load tanninases, resulting in a tanninase immobilized on the modified black tea residue carrier as a clarifying agent. First, furfural is used to modify the black tea residue. Then, furfural-modified black tea residue is used as a stabilizer, along with glycidyl methacrylate and allyl glycidyl ether monomers, N,N-methylenebisacrylamide crosslinking agent, and azobisisobutyronitrile initiator to prepare a Pickering emulsion. A polymer porous material with a macroporous structure is obtained using the Pickering emulsion template method. This material is then used as a carrier to load tanninases, ultimately yielding the clarifying agent.

[0020] The reason for using furfural to modify black tea residue in this invention is twofold. First, furfural can undergo a phenolic condensation reaction with the polyphenols abundant in black tea residue. While retaining the active phenolic hydroxyl groups in the polyphenols, it can also immobilize the polyphenols in a clarifying agent. When the extract is clarified with the clarifying agent, the polyphenols in the black tea residue can adsorb and remove proteins, iron ions, etc., from the extract, which helps reduce the formation of precipitation in the oral liquid. Second, modifying black tea residue with furfural can improve the hydrophobic properties of the surface of the black tea residue, making it suitable as a stabilizer to prepare Pickering emulsions, thereby creating an immobilized carrier capable of loading tanninases. The loading of tanninases can cleave the ester bonds of gallic acid on catechins in the extract, releasing gallic acid. The gallic acid anion can compete with polyphenols for caffeine, forming water-soluble substances with smaller molecular weights, thus reducing precipitation. Furthermore, the dissociated gallic acid also has astringent and hemostatic effects and will not affect the efficacy of the oral liquid. Therefore, the oral liquid clarified by the clarifying agent in this invention is less prone to precipitation during storage, and has excellent storage stability while maintaining good therapeutic effects.

[0021] Preferably, the particle size of the black tea residue powder in step (1) is 0.5 to 3 μm.

[0022] Preferably, in step (2), the mass ratio of black tea residue powder to furfural is 10:0.5-1; the pH of the system after adding hydrochloric acid solution is 1-3; the heating temperature during stirring is 60-70℃, and the reaction time is 2-3h.

[0023] Preferably, in step (3), the mass-to-volume ratio of furfural-modified black tea residue to deionized water is 10-15 g: 30-40 mL; in step (4), the mass-to-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile, and liquid paraffin is 3-5 g: 3-5 g: 3-5 g: 0.1-0.3 g: 60-70 mL; and in step (5), the volume ratio of oil phase to water phase is 30-40: 60-70.

[0024] Preferably, in step (6), the mass concentration of the tanninase solution is 0.05-0.1%, the mass-volume ratio of the carrier to the tanninase solution is 0.3-0.5 g: 5 mL, the shaking reaction temperature is 30-45 °C, and the reaction time is 3-5 h.

[0025] Preferably, the amount of clarifying agent used in the extract is 5-10 g / L.

[0026] Therefore, the present invention has the following beneficial effects:

[0027] (1) Using rice wine to extract black tea at high temperature to make oral liquid can effectively treat thrombocytopenic purpura, allergic purpura and capillary bleeding caused by thrombocytopenia caused by primary diseases; it can be effective in three days and can be cured in two weeks, with a high efficacy rate; (2) Black tea and rice wine, which are raw materials, are both food. Using food to replace medicine as raw materials has less toxic side effects.

[0028] (3) The oral liquid is made by high-temperature extraction and clarification, which is absorbed quickly, has stable quality, is convenient to carry and take, and is easy to store.

[0029] (4) Modify the remaining black tea residue after extraction to make a carrier to load tannins, and make tannins immobilized by the modified black tea residue carrier as a clarifying agent to clarify the extract. The resulting oral liquid is not prone to precipitation during storage and has good efficacy and excellent storage stability. Detailed Implementation

[0030] The present invention will now be further described in conjunction with specific embodiments.

[0031] In this invention, unless otherwise specified, the equipment and raw materials used are commercially available or commonly used in the field. The methods in the following embodiments, unless otherwise specified, are conventional methods in the field.

[0032] The black tea used in the various embodiments of the present invention is commercially available Yunnan Zhenjiang premium black tea, and the yellow wine is Shaoxing yellow wine.

[0033] General Implementation Examples:

[0034] An oral liquid for treating purpura, the preparation method of which is as follows:

[0035] (1) High temperature extraction: Add black tea to rice wine, with a ratio of 1g black tea to 20-30mL rice wine. Boil and extract for 1-3 hours. After filtration, obtain black tea residue and extract.

[0036] (2) Preparation of clarifying agent:

[0037] A) The extracted black tea residue is dried and then pulverized using an ultra-micro pulverizer to obtain black tea residue powder with a particle size of 0.5–3 μm;

[0038] B) Add black tea residue powder and furfural to ethanol, with a mass ratio of black tea residue powder to furfural of 10:0.5-1. After stirring and dispersing evenly, add hydrochloric acid solution to adjust the pH of the system to 1-3. Stir and react at 60-70℃ for 2-3 hours. Then filter, wash and dry the product to obtain furfural modified black tea residue.

[0039] C) Disperse furfural-modified black tea residue in deionized water to obtain an aqueous phase; the mass-to-volume ratio of furfural-modified black tea residue to deionized water in the aqueous phase is 10-15 g: 30-40 mL.

[0040] D) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain an oil phase; the mass-to-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile, and liquid paraffin in the oil phase was 3-5 g: 3-5 g: 3-5 g: 0.1-0.3 g: 60-70 mL;

[0041] E) The oil phase is added dropwise to the aqueous phase under stirring, with a volume ratio of oil phase to aqueous phase of 30-40:60-70. After stirring and homogenizing, an emulsion is obtained. The emulsion is heated at 60-80℃ for 18-24 hours. The product is dried to obtain the carrier.

[0042] F) Add tanninase to phosphate buffer solution with pH 4-6 to obtain tanninase solution with a mass concentration of 0.05-0.1%; add carrier to tanninase solution, the mass-to-volume ratio of carrier to tanninase solution is 0.3-0.5 g: 5 mL, shake and react at 30-45℃ for 3-5 h, then filter, wash and dry the product to obtain the clarifying agent;

[0043] (3) Clarification of the extract: Add a clarifying agent to the extract at a concentration of 5-10 g / L, stir and react at 40-50°C for 2-4 hours, and then filter to obtain the oral liquid for treating purpura.

[0044] Example 1:

[0045] An oral liquid for treating purpura, the preparation method of which is as follows:

[0046] (1) High temperature extraction: Add black tea to rice wine, the ratio of black tea to rice wine is 1g:25mL, boil and extract for 2 hours, filter to obtain black tea residue and extract;

[0047] (2) Preparation of clarifying agent:

[0048] A) The extracted black tea residue was dried and pulverized using an ultra-micro pulverizer to obtain black tea residue powder with a particle size of 1.3 μm;

[0049] B) Add black tea residue powder and furfural to ethanol, with a mass ratio of black tea residue powder to furfural of 10:0.8. After stirring and dispersing evenly, add 1 mol / L hydrochloric acid solution to adjust the pH of the system to 1.8. Stir the reaction at 65℃ for 2.5 h, then filter, wash and dry the product to obtain furfural modified black tea residue.

[0050] C) Disperse furfural-modified black tea residue in deionized water to obtain an aqueous phase; the mass-to-volume ratio of furfural-modified black tea residue to deionized water in the aqueous phase is 12g:35mL.

[0051] D) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain the oil phase; the mass-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile and liquid paraffin in the oil phase was 4 g:4 g:4 g:0.2 g:65 mL;

[0052] E) The oil phase is added dropwise to the aqueous phase under stirring, with a volume ratio of oil phase to aqueous phase of 35:65. After stirring and homogenizing, an emulsion is obtained. The emulsion is heated at 70°C for 24 hours. The product is dried to obtain the carrier.

[0053] F) Add tanninase (Fluka, 239 U / g) to phosphate buffer at pH 5 to obtain a tanninase solution with a mass concentration of 0.08%; add the carrier to the tanninase solution at a mass-volume ratio of 0.4 g: 5 mL, shake and react at 40 °C for 4 h, then filter, wash and dry the product to obtain the clarifying agent;

[0054] (3) Clarification of the extract: A clarifying agent is added to the extract at a concentration of 8 g / L. The mixture is stirred at 45°C for 3 hours and then filtered to obtain the oral liquid used to treat purpura.

[0055] Example 2:

[0056] An oral liquid for treating purpura, the preparation method of which is as follows:

[0057] (1) High temperature extraction: Add black tea to rice wine, the ratio of black tea to rice wine is 1g:25mL, boil and extract for 2 hours, filter to obtain black tea residue and extract;

[0058] (2) Clarification of the extract: The clarifying agent prepared in Example 1 was added to the extract. The amount of the clarifying agent in the extract was 10 g / L. The mixture was stirred at 45°C for 3 h. After filtration, the oral liquid for treating purpura was obtained.

[0059] Example 3:

[0060] An oral liquid for treating purpura, the preparation method of which is as follows:

[0061] (1) High temperature extraction: Add black tea to rice wine, the ratio of black tea to rice wine is 1g:25mL, boil and extract for 2 hours, filter to obtain black tea residue and extract;

[0062] (2) Clarification of the extract: The clarifying agent prepared in Example 1 was added to the extract. The amount of the clarifying agent in the extract was 5 g / L. The mixture was stirred at 45°C for 3 h. After filtration, the oral liquid for treating purpura was obtained.

[0063] Comparative Example 1 (Extraction of black tea with water):

[0064] The difference between Comparative Example 1 and Example 1 is that the high-temperature extraction method in step (1) is as follows: black tea is added to water, the ratio of black tea to water is 1g:25mL, boiled and extracted for 3h, and then filtered to obtain black tea residue and extract; the rest is the same as in Example 1.

[0065] Comparative Example 2 (without clarification of the extract):

[0066] An oral liquid for treating purpura is prepared by adding black tea to rice wine at a ratio of 1g:25mL, boiling and extracting for 3 hours, and then filtering to obtain the oral liquid for treating purpura.

[0067] Comparative Example 3 (modification of black tea residue without furfural):

[0068] The difference between Comparative Example 3 and Example 1 is that the clarifying agent used is prepared by the following method:

[0069] A) The extracted black tea residue was dried and pulverized using an ultra-micro pulverizer to obtain black tea residue powder with a particle size of 1.3 μm;

[0070] B) Disperse the black tea residue powder in deionized water to obtain an aqueous phase; the mass-to-volume ratio of the black tea residue powder to the deionized water in the aqueous phase is 12g:35mL.

[0071] C) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain the oil phase; the mass-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile and liquid paraffin in the oil phase was 4 g:4 g:4 g:0.2 g:65 mL;

[0072] D) The oil phase is added dropwise to the aqueous phase under stirring, with a volume ratio of oil phase to aqueous phase of 35:65. After stirring and homogenizing, an emulsion is obtained. The emulsion is heated at 70°C for 24 hours. The product is dried to obtain the carrier.

[0073] E) Add tanninase to phosphate buffer at pH 5 to obtain a tanninase solution with a mass concentration of 0.08%; add the carrier to the tanninase solution at a mass-to-volume ratio of 0.4 g: 5 mL, shake and react at 35 °C for 4 h, then filter, wash and dry the product to obtain the clarifying agent.

[0074] Everything else is the same as in Example 1.

[0075] Comparative Example 4 (using furfural-modified black tea residue as a clarifying agent):

[0076] The difference between Comparative Example 4 and Example 1 is that the clarifying agent used is prepared by the following method:

[0077] A) The extracted black tea residue was dried and pulverized using an ultra-micro pulverizer to obtain black tea residue powder with a particle size of 1.3 μm;

[0078] B) Add black tea residue powder and furfural to ethanol, with a mass ratio of black tea residue powder to furfural of 10:0.8. After stirring and dispersing evenly, add 1 mol / L hydrochloric acid solution to adjust the pH of the system to 1.8. Stir and react at 65°C for 2.5 h. Then filter, wash and dry the product to obtain the clarifying agent.

[0079] Everything else is the same as in Example 1.

[0080] Comparative Example 5 (using tanninase directly as a clarifying agent):

[0081] The difference between Comparative Example 5 and Example 1 is that tanninase was used directly as the clarifying agent, while the rest were the same as in Example 1.

[0082] Comparative Example 6 (without tanninase loaded on the porous carrier):

[0083] The difference between Comparative Example 6 and Example 1 is that the clarifying agent used is prepared by the following method:

[0084] A) The extracted black tea residue was dried and pulverized using an ultra-micro pulverizer to obtain black tea residue powder with a particle size of 1.3 μm;

[0085] B) Add black tea residue powder and furfural to ethanol, with a mass ratio of black tea residue powder to furfural of 10:0.8. After stirring and dispersing evenly, add 1 mol / L hydrochloric acid solution to adjust the pH of the system to 1.8. Stir the reaction at 65℃ for 2.5 h, then filter, wash and dry the product to obtain furfural modified black tea residue.

[0086] C) Disperse furfural-modified black tea residue in deionized water to obtain an aqueous phase; the mass-to-volume ratio of furfural-modified black tea residue to deionized water in the aqueous phase is 12g:35mL.

[0087] D) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain the oil phase; the mass-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile and liquid paraffin in the oil phase was 4 g:4 g:4 g:0.2 g:65 mL;

[0088] E) The oil phase is added dropwise to the aqueous phase under stirring, with a volume ratio of oil phase to aqueous phase of 35:65. After stirring and homogenizing, an emulsion is obtained. The emulsion is heated at 70°C for 24 hours. The product is dried to obtain the clarifying agent.

[0089] Everything else is the same as in Example 1.

[0090] The therapeutic effects of the oral solutions prepared in the above embodiments and comparative examples were observed, and the results are shown in Table 1; the observation method was as follows:

[0091] Referring to the diagnostic criteria for allergic purpura and thrombocytopenic purpura in the "Standards for Diagnosis and Efficacy of Traditional Chinese Medicine Diseases", 100 patients meeting the criteria for acute and chronic allergic purpura and thrombocytopenic purpura were selected and randomly divided into two groups of 50 each. The two groups were administered the oral liquid prepared in Example 1 and Comparative Example 1, respectively, and their efficacy was observed. The administration method was: 20 mL twice daily for two weeks. The efficacy criteria were: cured: complete disappearance of ecchymosis and petechiae, no bleeding points, and platelet count increased to over 100,000; significantly effective: most of the ecchymosis and petechiae disappeared, with occasional bleeding points, and platelet count increased to 70,000-90,000; effective: partial disappearance of ecchymosis and petechiae, and an increase in platelet count; ineffective: no disappearance of ecchymosis and petechiae, and no increase in platelet count.

[0092] Table 1: Observation results of the efficacy of oral liquid in treating purpura.

[0093] Cured (case) Significant effect (example) Valid (example) Invalid (example) Effectiveness (%) Example 1 24 16 7 3 94 Comparative Example 1 3 12 18 17 66

[0094] As can be seen from Table 1, the oral liquid prepared by the method of the present invention in Example 1 has good efficacy for allergic purpura and thrombocytopenic purpura, with a high efficacy rate; while in Comparative Example 1, water was used instead of rice wine to extract black tea, and the efficacy rate was significantly lower than that in Example 1. This indicates that the formula of the present invention can effectively treat thrombocytopenic purpura, allergic purpura, and capillary bleeding caused by thrombocytopenia due to primary diseases.

[0095] The storage stability of the oral liquids prepared in the above embodiments and comparative examples was tested. The test method was as follows: the oral liquids prepared in each embodiment and comparative example were stored at room temperature for 3 months, and samples were taken every 15 days to test their turbidity. The results are shown in Table 2.

[0096] Table 2: Results of oral liquid storage stability test.

[0097]

[0098]

[0099] As shown in Table 2, the oral liquids prepared by clarifying the extracts using the clarifying agent of this invention in Examples 1-3 exhibit good storage stability, with minimal turbidity change and only trace amounts of precipitation after 3 months of storage. In contrast, Comparative Example 2, which did not use a clarifying agent to clarify the extracts, showed a significantly lower storage stability compared to the examples. After 3 months of storage, more precipitation occurred, and the turbidity increased significantly, affecting the quality of the oral liquid. In Comparative Example 3, the clarifying agent was not modified with furfural during preparation, resulting in a lack of furfural's surface hydrophobic modification effect. Consequently, the clarifying agent did not effectively stabilize the Pickering emulsion, making it difficult to form a stable emulsion. This affected the formation of the porous carrier, leading to poor loading of tannins and reduced adsorption of components such as proteins, polysaccharides, and metal ions in the extract. This resulted in poor clarification, and the storage stability of the clarified oral liquid decreased compared to the examples, with more precipitation occurring after 3 months of storage. In Comparative Example 4, furfural-modified black tea residue was used as a clarifying agent, but it was not made into a porous carrier and loaded with tannins. This lack of porous carrier adsorption and tannins' effect on catechins resulted in a decrease in the clarifying effect compared to the examples, with a significant increase in turbidity in the oral liquid after 3 months of storage. In Comparative Example 5, only tannins were used as a clarifying agent, but they were not loaded onto the porous carrier of this invention. This lack of porous carrier adsorption led to poor clarification and difficulty in stable storage of the oral liquid. In Comparative Example 6, tannins were not loaded onto the porous carrier, thus lacking the tannins' effect on catechins. The clarification effect of the clarifying agent was also poor, with significant precipitation occurring in the oral liquid after storage.

Claims

1. A method for preparing an oral liquid for treating purpura, characterized by the following steps: extracting black tea with rice wine at high temperature, filtering to obtain black tea residue and extract; adding a clarifying agent to the extract for clarification to obtain the final product; The preparation method of the clarifying agent is as follows: (1) Dry and pulverize the black tea residue into black tea residue powder; add it and furfural to ethanol, stir to disperse, add hydrochloric acid solution, heat and stir to react, filter, wash and dry the product and disperse it in deionized water to obtain an aqueous phase; (2) Glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide and azobisisobutyronitrile were added to liquid paraffin and stirred until homogeneous to obtain the oil phase; (3) The oil phase is added dropwise to the aqueous phase under stirring. After stirring and homogenizing, an emulsion is obtained. The reaction is heated at 60~80℃ for 18~24h. The product is dried to obtain the carrier. (4) Add tanninase to phosphate buffer solution with pH 4-6 to obtain tanninase solution; add carrier to tanninase solution, shake reaction, filter, wash and dry the product to obtain clarifying agent.

2. The method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, The high-temperature extraction method is as follows: add black tea to rice wine, boil and extract for 1-3 hours, and then filter to obtain black tea residue and extract.

3. The method for preparing an oral liquid for treating purpura according to claim 2, characterized in that, The ratio of black tea to rice wine is 1g: 20~30mL.

4. The method for preparing an oral liquid for treating purpura according to claim 2, characterized in that the extract... The clarification method is as follows: add a clarifying agent to the extract, stir and react at 40~50℃ for 2~4 hours, and then filter to obtain the oral liquid for treating purpura.

5. The method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, The particle size of the black tea residue powder in step (1) is 0.5~3μm.

6. The method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, In step (1), the mass ratio of black tea residue powder to furfural is 10:0.5~1; the pH of the system after adding hydrochloric acid solution is 1~3; the heating temperature during stirring is 60~70℃, and the reaction time is 2~3h.

7. The method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, In step (1), the mass-to-volume ratio of furfural-modified black tea residue to deionized water is 10-15g:30-40mL; In step (2), the mass-to-volume ratio of glycidyl methacrylate, allyl glycidyl ether, N,N-methylenebisacrylamide, azobisisobutyronitrile and liquid paraffin is 3~5g:3~5g:3~5g:0.1~0.3g:60~70mL; In step (3), the volume ratio of the oil phase to the water phase is 30~40:60~70.

8. The method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, In step (4), the mass concentration of the tanninase solution is 0.05~0.1%, the mass-volume ratio of the carrier to the tanninase solution is 0.3~0.5g:5mL, the shaking reaction temperature is 30~45℃, and the reaction time is 3~5h.

9. A method for preparing an oral liquid for treating purpura according to claim 1, characterized in that, The amount of clarifying agent used in the extract is 5~10g / L.

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